A specialist course will be organized on CRYOPRESERVATION OF GAMETES, EMBRYOS AND STEM CELLS. Tuesday 20 Wednesday 21- Thursday 22 september 2016

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1 A specialist course will be organized on CRYOPRESERVATION OF GAMETES, EMBRYOS AND STEM CELLS Tuesday 20 Wednesday 21- Thursday 22 september 2016 Organized by : Doctoral Schools Ugent Rep-Biotech Ghent University : Prof. Ann Van Soom and Prof. E. Van den Abbeel University of Antwerp : Prof. P.E.J. Bols Free University of Brussels: Prof. J. Smitz and Prof. D. Stoop Location : Faculty of Veterinary Medicine Ghent University Department of Reproduction, Obstetrics and Herd Health Salisburylaan 133, 9820 Merelbeke

2 Practical information : HOW TO COME TO GENT FROM BRUSSELS (NATIONAL AIRPORT) Gent is easily reached by train (Gent Sint-Pieters-station) from Brussels airport (Zaventem). Direct trains run from Brussels airport, or you can change in Brussels. But be aware that if you change in Brussels some trains take much longer than others. At Brussels airport you go to the lowest level (by following the signs) and there you can take the train. Should you want to look for possible train connections: there is the official web-site from the Belgian Railways: From: BRUXELLES-NAT-AIRPORT to: GENT-SINT-PIETERS enter when you want to leave or arrive click on the "timetable and buy tickets" button. You can also select to see this web-site in Dutch, French or German (top of page at the right : NL, FR or DE). How to come from the station in Gent to the city centre The cheapest way to go from the railway station to the centre of Ghent is by tram. Several trams go to the centre directly (e.g. tram nr. 1). There is an information desk next to the railway station exit (at your left). By bus is another option, but this might be a bit more complicated. You can of course also go by taxi, but this is a lot more expensive.

3 Accomodation : We advise people to book their room on booking.com or trivago.be in the city of Ghent. Please book your rooms early, since September is a very popular period to visit the city. Transport will be organized each morning to the faculty from the Kiss & Ride zone at the Jules de Bruyckerdreef in Ghent -see map below. If you book a room for instance in the IBIS Cathedral hotel, it will be a 15 min walk to this zone.

4 TESTIS AND SEMEN CRYOPRESERVATON Day 1 : Tuesday 20 september u Registration u Introduction : Ann Van Soom u Key note lecture : Cryopreservation for mammalian embryologist. Henri Woelders Nederland u: Coffee break Session 1: Freezing of semen and testicular tissue : theory Cryopreservation and banking of dog semen Wenche Farstad Oslo Norway Dry-state preservation of mammalian sperm - Takehito Kaneko, Graduate School of Medicine, Kyoto University, Kyoto, Japan Cryopreservation of porcine testicular tissue -Kazuhiro Kikuchi; Japan Lunch Vitrification of semen from domestic and wild felids-bill Swanson- Cincinnati Zoo & Botanical Garden USA Session 2: Wetlab in freezing of animal semen and semen collection Demonstration of collection of stallion semen Jan Govaere Video on collection of felid semen- Bill Swanson Coffee break exhibition Allocation of students in three groups of 10 Parallel sessions of 30 minutes each u IVF lab Semen lab Int Semen lab Collection semen from bull testes (TESE/PESA) Petra/Isabel Demonstration of CASA. Eline/Feline Semen cryopreservation in straws and vitrification in pellets (Cyriel? Take Kaneko? ) 1700 u Transport back to the city of Gent Diner (on your own)

5 OVARY AND OOCYTE CRYOPRESERVATON Day 2 : Wednesday 21 september u Key note lecture : Vitrification of reproductive cells : as clear as a glass? Etienne Van den Abbeel (Ugent) u: Coffee break Session 3: Freezing of oocytes and ovarian tissue Cryopreservation of bovine preantral follicles Peter Bols UA Cryopreservation of human oocytes Dominic Stoop VUB Cryopreservation of human ovarian tissue Ingrid Segers VUB Cryopreservation of porcine ovarian tissue Dr. Kikuchi Japan Lunch Session 4: Wetlab in collection of oocytes/ovarian tissue and cryopreservation Practical introduction on how to cryopreserve oocytes, with demonstration of equipment necessary (Etienne?) u Coffee break exhibition Allocation of students in three groups of 10 Parallel sessions of 30 minutes each u IVF lab IVF lab Collection oocytes from bovine ovaries Petra/Isabel Collection of human ovarian strips. Annick Semen lab Demonstration of vitrification (Nerea/Etienne/??) 1700 u Transport back to the city of Gent Social programme VIZIT

6 EMBRYO AND STEM CELL CRYOPRESERVATON Day 23 : Thursday 22 september u Key note lecture : Cryopreservation of stem cells. Catharina De Schauwer u Coffee break Session 5: Practical aspects on cryopreservation of embryo, gametes and stem cells Cryopreservation of bovine embryos Erik Mullaart Cryopreservation of human embryos and blastocysts Liesbeth Van Landuyt Cryopreservation of porcine oocytes Tamas Somfai -Japan Oncofertility: female and male fertility preservation -Kelly Tilleman - Ugent Lunch Closing lecture and discussion: The importance of biobanking of gametes and stem cells for preservation of endangered species -Veerle Ronsse- iarks Discussion and feedback/evaluation of the course 1500u Transport back to the city of Gent Diner (on your own)