EXPERIMENT 4. This lab will meet on Monday October 22 nd and Tuesday October 23 rd in Science Center 210.

Size: px
Start display at page:

Download "EXPERIMENT 4. This lab will meet on Monday October 22 nd and Tuesday October 23 rd in Science Center 210."

Transcription

1 EXPERIMENT 4 Synthesis and Analysis of CdSe Quantum Dot Nanocrystals This lab will meet on Monday October 22 nd and Tuesday October 23 rd in Science Center 210. Before You Come to Lab: Read the entire lab, paying careful attention to the safety precautions. Print a copy and bring it with you to lab. Complete the pre-lab, which is the last page of the lab report, and give the pre-lab to your TF as you enter the lab. On Your Assigned Lab Day Arrive on time! You will not be admitted to lab if the Head TF has already started the safety training. Wear proper attire and bring your goggles (see safety manual). Listen carefully to the pre-lab talk given by your TF. Before You Leave the Lab Have your TF check your lab bench for cleanup. Submit your data and lab report to your TF. Wash your hands thoroughly before leaving the lab. Grading: Prelab: / 10 Lab Report: / 20 Safety: / 3 Cleanup: / 2 Total: / 35 1

2 EXPERIMENT 4 Synthesis and Analysis of CdSe Quantum Dot Nanocrystals This experiment is adapted from E. M. Boatman, G. C. Lisensky, and K. J. Nordell, "A Safer, Easier, Faster Synthesis for CdSe Quantum Dot Nanocrystals," J. Chem. Educ., 82, (2005) Introduction Quantum dots are semiconducting nanocrystals whose properties vary with size. CdSe nanocrystals have a crystal lattice of alternating cadmium and selenium atoms that exhibits the same structure as bulk CdSe. When a photon is absorbed by the bulk crystal, an electron-hole pair is created and the particles maintain a characteristic distance apart. However, when the size of the crystal is small enough (less than 112 Å in diameter for CdSe), the electron-hole pair can no longer achieve the desired distance and the particles become confined to a box. As a result, these nanocrystals demonstrate quantum mechanical behavior and their transition energies can be explained using the particle in a box model, where the electron is the particle and the nanocrystal is the box. The particle in a box model, modified for the case of a sphere, is shown below, E n hc max h 2 (n f 2 n i 2 ) 8m e * R 2 where h = Planck s constant R = radius of the nanocrystal n f = the final energy level n i = the initial energy level m e * = effective mass of an electron. The effective mass of an electron must be used, because conduction electrons have a smaller mass than regular electrons. Effective mass varies by material and is 0.13m 0 (m 0 = mass of an electron) for CdSe. In this experiment, you will synthesize CdSe quantum dots, varying in diameter from approximately 1.8 nm to 4.0 nm. You will then measure the absorbance and emission spectra of the quantum dots and use max to calculate their size. 2

3 Safety Precautions Proper PPE Wear proper attire You must wear a lab coat, chemical splash goggles, and gloves while in the lab. Wear proper heat-resistant gloves for transferring QD solution Chemicals Several of the chemicals used in this experiment can be hazardous if handled improperly. It is extremely important that all work be carried out in the hood and that the sash be kept as low as possible while you are working. Take your time to do the experiment and always think carefully about what you are doing. If you have any questions, ask your TF. Octadecene - Octadecene vapor should not be inhaled, and contact with skin and eyes should be avoided. Trioctylphosphine - Trioctylphosphine is corrosive and causes burns. Cadmium oxide - Cadmium oxide is highly toxic and cadmium compounds are known to be human carcinogens if inhaled or swallowed. Oleic acid - Oleic acid is air, light, and heat sensitive and irritating to eyes, the respiratory system, and the skin. Elemental selenium is toxic by inhalation, by absorption through the skin, and the effects of ingestion are cumulative. Heat You will heat your reaction mixture to 225C (more than double the boiling point of water!) and transfer hot solution from one vessel to another. You should wear nitrile gloves and heat resistant gloves during this step and focus on transferring the solution in a slow and controlled manner to avoid spilling any hot solution on yourself or others. 3

4 Procedure Practice using a Pasteur Pipet In this experiment, you will be using a Pasteur pipet to transfer your reaction mixture (at very high temperature!) from a round bottom flask to a test tube. It is important to have the flask clamped at a height that makes it comfortable and safe to transfer liquid from the flask to the test tube. Set up your apparatus as shown below using a clean 25 ml round bottom flask. 25 ml round bottom flask, clamped to rod. Heating mantle Hot-plate stirrer Only use for stirring Lab Jack Fill the flask with ~ 10 ml of distilled water. Place a test tube holder and one test tube next to the set up. Using a Pasteur pipet, practice pipetting from the flask to the test tube. Adjust the height of the flask until it is at the most comfortable height for pipetting. Add a clamp above the rbf for holding a thermometer. When you are done practicing, remove the test flask by lowering the lab jack (Do not change the height of the clamp!). Dump out the water and put the flask on a rack to dry. Preparation of CdSe Quantum Dots Obtain from your TF a 25 ml round bottom flask containing approximately 13 mg of CdO. Clamp this flask above the heating mantle. Using a 1 ml syringe, add 0.6 ml oleic acid to the flask. Then add 10 ml octadecene using a graduated cylinder. Insert a thermometer capable of measuring 225 C and turn the variac to 70 to begin heating the flask. While you are waiting for the reaction mixture to heat up, get a test tube rack and fill it with ten test tubes. Obtain a clean Pasteur pipet. 4

5 A M selenium stock solution has been prepared for you by dissolving elemental selenium in octadecene and trioctylphosphine. As soon as the temperature of your reaction mixture reaches 225 C, notify your TF. Using a syringe, your TF will transfer 1 ml of the room temperature selenium stock solution to the 225 C cadmium solution. Because the size of the nanoparticles depends on reaction time, start timing when the selenium solution is added. Use a 9-inch Pasteur pipet to carefully remove approximately 1 ml samples at frequent intervals and quench by squirting into separate test tubes. You should obtain nine or ten samples within two to three minutes. Observing Fluorescence Place your test tubes in the UV box and record the color of the emitted light for each of your samples. Note the intensity of the light. Measuring Absorbance and Emission Obtain a cuvette holder and 5 cuvettes and caps. Choose your five most distinctly colored samples and, using clean pipettes, transfer your quantum dots to the cuvettes and cap them. Record a set of absorbance spectra and emission spectra for your samples using a Vernier spectrometer (your TF will demonstrate) and note the maximum wavelength. Print a copy of each set of spectra and attach it to your lab report. Sample Absorbance max Emission max 5

6 Clean-up and Waste Disposal Dump all waste into the designated waste container in the hood. Thoroughly clean all glassware with soapy water over a waste beaker. Rinse with distilled water. When you are done, dump the contents of waste beaker into the designated waste container. Place washed glassware into the blue bins. Thoroughly clean the surface of your bench using paper towels. Dispose of all solid waste (contaminated gloves and paper towels, empty cuvettes and caps) in the solid waste container. 6

7 Lab Report 25 points 1. What colors did you observe using visible light? Using ultraviolet light? 2. Do the wavelengths get longer or shorter as the particles grow? Why is this the case? 3. What relationship do you observe between the absorption wavelength and the emission wavelength for each sample? Briefly explain. 4. How can solutions containing particles with identical chemical composition (all of the samples are CdSe) have different colors? 7

8 5. Use the particle in a box model and the wavelength of maximum absorbance to calculate the diameter of your quantum dots. Do the calculations correspond to the expected size range of the particles? Sample Particle Size 6. A major goal in synthesizing nanocrystals is the production of monodisperse particles (monodisperse particles exhibit a relatively narrow size distribution.). Were your samples monodisperse? How do you know? 8

9 Prelab 1. Briefly describe one application of quantum dots that you learned about in class or on your own. 2. Using the particle in a box model, calculate the size of a CdS quantum dot that has max = 500 nm. (m e * = 0.19 for CdS) 3. Name three safety hazards in this experiment and the precautions you will take to minimize the risk of an accident. 9

Synthesis and Characterization of Gold Nanoparticles

Synthesis and Characterization of Gold Nanoparticles Collect Synthesis and Characterization of Gold Nanoparticles Apparatus Amount Apparatus Amount Wash with aqua regia in hood: 50 ml round-bottomed flask (2016/03/14 revised) 1 Cuvettes 2 Condenser 1 Stir

More information

DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included.

DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included. DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included. Dyeing of textiles has been practiced for thousands of years with the first

More information

Spectroscopic Determination of the Concentration of Manganese

Spectroscopic Determination of the Concentration of Manganese Spectroscopic Determination of the Concentration of Manganese Objectives The objective of this laboratory is to use absorption spectroscopy to determine the amount of Mn 2+ in an aqueous solution. Background

More information

Qualitative Analysis of Group 1 Cations

Qualitative Analysis of Group 1 Cations Qualitative Analysis of Group 1 Cations Objectives The objectives of this laboratory are to follow a classic analytical scheme to separate and identify the ions in a known mixture of Group 1 cations, and

More information

How are drugs developed?

How are drugs developed? How are drugs developed? Take part in the research of a drug Experiment workshop Student guide 1 Introduction Many stages are involved in the discovery and development of medicines. One of the most important

More information

Exploring Energy Changes: Endothermic and Exothermic Reactions. Introduction

Exploring Energy Changes: Endothermic and Exothermic Reactions. Introduction Exploring Energy Changes: Endothermic and Exothermic Reactions Introduction The story of chemistry is the story of change physical change, chemical change, and energy change. Energy in the form of heat

More information

THE IRON(III) THIOCYANATE REACTION SYSTEM

THE IRON(III) THIOCYANATE REACTION SYSTEM Experiment 7 THE IRON(III) THIOCYANATE REACTION SYSTEM Prepared by Ross S. Nord, Chemistry Department, Eastern Michigan University PURPOSE To investigate a novel reaction system by utilizing a spectrophotometer.

More information

How Can the Percent Composition of a Salt, Sand and Iron Mixture be Determined?

How Can the Percent Composition of a Salt, Sand and Iron Mixture be Determined? Lab 2 Name How Can the Percent Composition of a Salt, Sand and Iron Mixture be Determined? Pre-Lab Assignment This written pre-lab is worth 15% (3 points) of your lab report grade and must be turned in

More information

Synthesis, Isolation, and Purification of an Ester

Synthesis, Isolation, and Purification of an Ester Synthesis, Isolation, and Purification of an Ester AP Chemistry Laboratory Introduction An ester is a chemical compound that is formed when an organic acid reacts with an alcohol. Esters frequently have

More information

experiment5 Understanding and applying the concept of limiting reagents. Learning how to perform a vacuum filtration.

experiment5 Understanding and applying the concept of limiting reagents. Learning how to perform a vacuum filtration. 81 experiment5 LECTURE AND LAB SKILLS EMPHASIZED Synthesizing an organic substance. Understanding and applying the concept of limiting reagents. Determining percent yield. Learning how to perform a vacuum

More information

Dr. Caddell A Cycle of Copper Reactions Chemistry 101. The Law of Conservation of Mass: A Cycle of Copper Reactions

Dr. Caddell A Cycle of Copper Reactions Chemistry 101. The Law of Conservation of Mass: A Cycle of Copper Reactions The Law of Conservation of Mass: A Cycle of Copper Reactions EQUIPMENT You will need one 250 ml beaker, one hot plate, beaker tongs, a 50 ml graduated cylinder, a stirring rod, a wash bottle full of D.I.

More information

Standard Operating Procedures for Hazardous Chemicals

Standard Operating Procedures for Hazardous Chemicals Standard Operating Procedures for Hazardous Chemicals Title of Procedure: Nitric Acid Dilutions for Flame AA Date: 3/29/2012 Date of Last Review: Principal Investigator: Benny Freeman Lab Location: PRC

More information

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy

Determining the Quantity of Iron in a Vitamin Tablet. Evaluation copy Determining the Quantity of Iron in a Vitamin Tablet Computer 34 As biochemical research becomes more sophisticated, we are learning more about the role of metallic elements in the human body. For example,

More information

What is a Chemical Reaction?

What is a Chemical Reaction? Lab 8 Name What is a Chemical Reaction? Macroscopic Indications and Symbolic Representations Pre-Lab Assignment This written pre-lab is worth 25% (5 POINTS) of your lab report grade and must be turned

More information

Determination of Molecular Mass by Freezing Point Depression

Determination of Molecular Mass by Freezing Point Depression Determination of Molecular Mass by Freezing Point Depression Objectives: To determine the molecular mass of an unknown solid using the colligative property of freezing point depression. Background: When

More information

Dispose of all materials in the proper waste containers which are provided in the laboratory.

Dispose of all materials in the proper waste containers which are provided in the laboratory. THE EXTRACTION AND IDENTIFICATION OF ARTIFICIAL FOOD COLORS 2009, 2003, 2000, 1997, 1975 by David A. Katz. All Rights reserved. Reproduction permitted for education use provided original copyright is included.

More information

2 To use calorimetry results to calculate the specific heat of an unknown metal. 3 To determine heat of reaction ( H) from calorimetry measurements.

2 To use calorimetry results to calculate the specific heat of an unknown metal. 3 To determine heat of reaction ( H) from calorimetry measurements. Calorimetry PURPOSE To determine if a Styrofoam cup calorimeter provides adequate insulation for heat transfer measurements, to identify an unknown metal by means of its heat capacity and to determine

More information

Title: Training Lab 1 Chemistry 11100, Fall Use of a balance a balance is used to measure the mass of solids in the chemistry lab.

Title: Training Lab 1 Chemistry 11100, Fall Use of a balance a balance is used to measure the mass of solids in the chemistry lab. Title: Training Lab 1 Chemistry 11100, Fall 2012 Introduction The purpose of this lab is to introduce you to a series of techniques that you will use in chemistry lab. For this lab, you will be using the

More information

Purification by Recrystallization

Purification by Recrystallization Experiment 2 Purification by Recrystallization Objectives 1) To be able to select an appropriate recrystallizing solvent. 2) To separate and purify acetanilide by recrystallization. 3) To compare the melting

More information

In this experiment, we will use three properties to identify a liquid substance: solubility, density and boiling point..

In this experiment, we will use three properties to identify a liquid substance: solubility, density and boiling point.. Identification of a Substance by Physical Properties 2009 by David A. Katz. All rights reserved. Permission for academic use provided the original copyright is included Every substance has a unique set

More information

Physical and Chemical Properties

Physical and Chemical Properties Physical and Chemical Properties Introduction Matter can be classified in different ways using physical and chemical properties. Physical properties include color, odor, density, hardness, structure, solubility,

More information

Experiment 10B DETERMINING THE MOLAR MASS OF A GAS

Experiment 10B DETERMINING THE MOLAR MASS OF A GAS Experiment 10B DETERMINING THE MOLAR MASS OF A GAS FV 3-31-16 MATERIALS: Dry 250 ml Erlenmeyer flask, piece of foil (~3 x 3 ), 800 ml beaker, 500 ml graduated cylinder, iron ring, ring stand, wire gauze,

More information

Molar Mass by Freezing Point Depression AP Chemistry Laboratory #4

Molar Mass by Freezing Point Depression AP Chemistry Laboratory #4 Catalog No. AP6356 Publication No. 6356A Molar Mass by Freezing Point Depression AP Chemistry Laboratory #4 Introduction A procedure for determining the molar mass of a substance is very useful to chemists.

More information

Household Acids and Bases

Household Acids and Bases Household Acids and Bases Computer 28 Many common household solutions contain acids and bases. Acid-base indicators, such as litmus and red cabbage juice, turn different colors in acidic and basic solutions.

More information

Household Acids and Bases

Household Acids and Bases Household Acids and Bases GRADE LEVEL INDICATORS Experiment Demonstrate that the ph scale (0-14) is used to measure acidity and classify substances or solutions as acidic, basic, or neutral. 21 Develop

More information

PHYSICAL AND CHEMICAL CHANGE EXPERIMENT 1

PHYSICAL AND CHEMICAL CHANGE EXPERIMENT 1 PURPOSE To investigate the criteria used to distinguish between physical and chemical changes in matter. 1 DEFINITIONS Chemical property, physical property, chemical change, physical change, conservation

More information

Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP)

Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP) Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP) Formaldehyde, formalin, paraformaldehyde solutions, and paraformaldehyde solids Principal Investigator: Room & Building #: Department:

More information

What is the Percent Copper in a Compound?

What is the Percent Copper in a Compound? Lab 9 Name What is the Percent Copper in a Compound? Pre-Lab Assignment Complete this pre-lab on this sheet. This written pre-lab is worth 15% (3 points) of your lab report grade and must be initialed

More information

Beware that ordinary prescription glasses do not provide adequate protection. This is also true with poorly fitting safety glasses.

Beware that ordinary prescription glasses do not provide adequate protection. This is also true with poorly fitting safety glasses. Ethidium Bromide Introduction Ethidium bromide (EtBr) is widely used for visualization of nucleic acids in electrophoretic gels. EtBr forms fluorescent complexes, by intercalation of DNA, which are readily

More information

Enzyme Pre-Lab. Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab.

Enzyme Pre-Lab. Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab. Enzyme Pre-Lab Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab. Background: In this investigation, you will study several

More information

Clock Reaction Revised: 9/28/15 CLOCK REACTIONS. Adapted from J. Chem. Ed. 2007, 84, ELETRONIC LABORATORY NOTEBOOK (ELN) INSTRUCTIONS

Clock Reaction Revised: 9/28/15 CLOCK REACTIONS. Adapted from J. Chem. Ed. 2007, 84, ELETRONIC LABORATORY NOTEBOOK (ELN) INSTRUCTIONS CLOCK REACTIONS Adapted from J. Chem. Ed. 2007, 84, 797 800. ELETRONIC LABORATORY NOTEBOOK (ELN) INSTRUCTIONS If you have not already done so, go to the eresources webpage and click on LabArchives (ELN).

More information

Water Lab. Objective: To distill samples of water that contains volatile and nonvolatile components.

Water Lab. Objective: To distill samples of water that contains volatile and nonvolatile components. Water Lab I. Distillation Hypothesis: Water can be purified by distillation. Objective: To distill samples of water that contains volatile and nonvolatile components. Materials and Equipment: Sodium chloride,

More information

# 12 Condensation Polymerization: Preparation of Two Types of Polyesters

# 12 Condensation Polymerization: Preparation of Two Types of Polyesters # 12 Condensation Polymerization: Preparation of Two Types of Polyesters Submitted by: Arturo Contreras, Visiting Scholar, Center for Chemical Education, Miami University, Middletown, OH; 1996 1997. I.

More information

Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry

Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry Micro Mole Rockets Hydrogen and Oxygen Mole Ratio As adapted from Flinn ChemTopic- Labs - Molar Relationships & Stoichiometry Introduction The combustion reaction of hydrogen and oxygen is used to produce

More information

Percent Composition of Hydrates

Percent Composition of Hydrates Name Class Date Percent Composition of Hydrates You are a research chemist working for a company that is developing a new chemical moisture absorber and indicator. The company plans to seal the moisture

More information

Determination of the Molar Mass of an Unknown Solid by Freezing Point Depression

Determination of the Molar Mass of an Unknown Solid by Freezing Point Depression Determination of the Molar Mass of an Unknown Solid by Freezing Point Depression GOAL AND OVERVIEW In the first part of the lab, a series of solutions will be made in order to determine the freezing point

More information

Solubility of a Salt Minneapolis Community and Technical College C1152 v.5.10

Solubility of a Salt Minneapolis Community and Technical College C1152 v.5.10 Solubility of a Salt Minneapolis Community and Technical College C1152 v.5.10 Solutions are homogeneous mixtures made up of one or more solutes dissolved in a solvent. The solvent is the component present

More information

Analysis of Hydrogen Peroxide A Redox Titration

Analysis of Hydrogen Peroxide A Redox Titration Analysis of Hydrogen Peroxide A Redox Titration Introduction Hydrogen peroxide is regarded as an "environmentally friendly" alternative to chlorine for water purification and wastewater treatment. Because

More information

H H H O. Pre-Lab Exercises Lab 6: Organic Chemistry. Lab 6: Organic Chemistry Chemistry 100. 1. Define the following: a.

H H H O. Pre-Lab Exercises Lab 6: Organic Chemistry. Lab 6: Organic Chemistry Chemistry 100. 1. Define the following: a. Lab 6: Organic hemistry hemistry 100 1. Define the following: a. ydrocarbon Pre-Lab Exercises Lab 6: Organic hemistry Name Date Section b. Saturated hydrocarbon c. Unsaturated hydrocarbon 2. The formula

More information

Chemistry 1215 Experiment #9 Copper and its Compounds

Chemistry 1215 Experiment #9 Copper and its Compounds Chemistry 1215 Experiment #9 Copper and its Compounds Objective The objective of this experiment is to take a piece of copper as efficiently as possible through a series of chemical reactions. The final

More information

THE ACTIVITY OF LACTASE

THE ACTIVITY OF LACTASE THE ACTIVITY OF LACTASE Lab VIS-8 From Juniata College Science in Motion Enzymes are protein molecules which act to catalyze the chemical reactions in living things. These chemical reactions make up the

More information

Plant Indicators for Acids and Bases Carole R. Goshorn

Plant Indicators for Acids and Bases Carole R. Goshorn SCIENCE EXPERIMENTS ON FILE Revised Edition 5.28-1 Plant Indicators for Acids and Bases Carole R. Goshorn Topic Acid/base indicators Time Preparation by teacher 50 minutes; Part B, 45 minutes! Safety Adult

More information

Schools Analyst Competition

Schools Analyst Competition Royal Society of Chemistry Analytical Division North West Region Schools Analyst Competition 28 th March 2006 Experimental Handbook Adran Cemeg - Department of Chemistry Prifysgol Cymru - University of

More information

DENSITY OF AQUEOUS SODIUM CHLORIDE SOLUTIONS

DENSITY OF AQUEOUS SODIUM CHLORIDE SOLUTIONS Experiment 3 DENSITY OF AQUEOUS SODIUM CHLORIDE SOLUTIONS Prepared by Ross S. Nord and Stephen E. Schullery, Eastern Michigan University PURPOSE Determine the concentration of an unknown sodium chloride

More information

Good Hope School Integrated Science New S.1 Summer Work

Good Hope School Integrated Science New S.1 Summer Work Good Hope School Integrated Science New S.1 Summer Work Section 1: Basic vocabulary in Secondary Science The following lists some English terms used in Secondary Science. analyze demonstrate laboratory

More information

Educational Objectives The student will determine the concentration of an unknown.

Educational Objectives The student will determine the concentration of an unknown. Spectrophotometric Analysis 2011 by L. Dickerson and H. Patterson Lab Type Greener Lab. Quantitative wet lab. Students work in pairs. Educational Objectives The student will determine the concentration

More information

Mixtures and Pure Substances

Mixtures and Pure Substances Unit 2 Mixtures and Pure Substances Matter can be classified into two groups: mixtures and pure substances. Mixtures are the most common form of matter and consist of mixtures of pure substances. They

More information

Standard Operating Procedure

Standard Operating Procedure Standard Operating Procedure FLAMMABLE LIQUIDS This standard operating procedure (SOP) is intended to provide general guidance on how to safely work with flammable liquids. This SOP is generic in nature

More information

Solubility Curve of Sugar in Water

Solubility Curve of Sugar in Water Solubility Curve of Sugar in Water INTRODUCTION Solutions are homogeneous mixtures of solvents (the larger volume of the mixture) and solutes (the smaller volume of the mixture). For example, a hot chocolate

More information

HOW CAN WE DETERMINE THE ACTUAL PERCENTAGE OF H 2 O 2 IN A DRUGSTORE BOTTLE OF HYDROGEN PEROXIDE

HOW CAN WE DETERMINE THE ACTUAL PERCENTAGE OF H 2 O 2 IN A DRUGSTORE BOTTLE OF HYDROGEN PEROXIDE HOW CAN WE DETERMINE THE ACTUAL PERCENTAGE OF H 2 O 2 IN A DRUGSTORE BOTTLE OF HYDROGEN PEROXIDE Introduction Hydrogen peroxide is regarded as an "environmentally friendly" alternative to chlorine for

More information

Evaluation copy. Fractional Distillation. computer OBJECTIVES MATERIALS

Evaluation copy. Fractional Distillation. computer OBJECTIVES MATERIALS Fractional Distillation Computer 8 An example of a simple distillation is the separation of a solution of salt and into two separate pure substances. When the salt solution is heated to boiling, vapor

More information

Accurately Diluting a Solution

Accurately Diluting a Solution Accurately Diluting a Solution Correct Use of Volumetric Transfer Pipettes Dr. David C. Stone 2005-2006 Department of Chemistry, University of Toronto All rights reserved. This is a print version of the

More information

Expt 44 Syntheses of Nylon & Polystyrene

Expt 44 Syntheses of Nylon & Polystyrene Expt 44 Syntheses of Nylon & Polystyrene Procedure adapted from K. L. Williamson, R. D. Minard, K. M. Masters Macroscale and Microscale rganic Experiments, 5th Ed. 2007 PreLab Question: 1. Name two (2)

More information

Austin Peay State University Department of Chemistry CHEM 1111. Copper Cycle

Austin Peay State University Department of Chemistry CHEM 1111. Copper Cycle Cautions Nitric acid and sulfuric acid are toxic and oxidizers and may burn your skin. Nitrogen dioxide gas produced is hazardous if inhaled. Sodium hydroxide is toxic and corrosive and will cause burns

More information

Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS

Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS Lab #9 Chem 100 Lab Experiment #9 - ACID/BASE INDICATORS Name: Purpose: In this laboratory we will investigate how indicators can be used to test for the presence of acids or bases in a number of common

More information

USING THE SPECTROPHOTOMETER TO ANALYZE A MIXTURE INTRODUCTION

USING THE SPECTROPHOTOMETER TO ANALYZE A MIXTURE INTRODUCTION USING THE SPECTROPHOTOMETER TO ANALYZE A MIXTURE INTRODUCTION Description This experiment is designed to introduce students to the spectrophotometer and the decisions that must be made in using it as a

More information

In order for no one to be harmed when working in lab the following rules apply:

In order for no one to be harmed when working in lab the following rules apply: Lab safety In order for no one to be harmed when working in lab the following rules apply: 1. When working in the lab you must always use protective clothing. Protective clothing consists of a lab coat

More information

Calcium Analysis by Atomic Absorption

Calcium Analysis by Atomic Absorption Calcium Analysis by Atomic Absorption In this experiment, your unknown calcium solution from last week's experiment will be analyzed for calcium by atomic absorption analysis and the results compared with

More information

CHM 130LL: Heat and Energy

CHM 130LL: Heat and Energy CHM 130LL: Heat and Energy WARNING: This lab uses cashews. Please inform your instructor BEFORE lab if you are allergic. Energy is defined as the ability to do work, and one form of energy is heat. Heat

More information

Laboratory and Safety Equipment Complete the chart below. Equipment Name Function perform chemical tests and reactions beaker.

Laboratory and Safety Equipment Complete the chart below. Equipment Name Function perform chemical tests and reactions beaker. Laboratory and Safety Equipment Complete the chart below. perform chemical tests and reactions beaker heat liquids beaker tongs pick up and move hot beakers Erlenmeyer flask perform reactions filtering

More information

Methylene Blue Kit Instruction Manual

Methylene Blue Kit Instruction Manual Methylene Blue Kit Instruction Manual Manual No. 209860, Revision E Instrument No. 209679 & 209694 2013 Fann Instrument Company Houston, Texas, USA All rights reserved. No part of this work covered by

More information

Solution Stoichiometry Quantitative Preparation of Aqueous Solutions

Solution Stoichiometry Quantitative Preparation of Aqueous Solutions Solution Stoichiometry Quantitative Preparation of Aqueous Solutions Introduction Page 1 of 11 In this week s lab, you and your lab partner will apply your prelab calculations to the preparation of aqueous

More information

Experiment 3 Determination of an Equilibrium Constant for the Iron (III) thiocynate Reaction

Experiment 3 Determination of an Equilibrium Constant for the Iron (III) thiocynate Reaction Experiment 3 Determination of an Equilibrium Constant for the Iron (III) thiocynate Reaction Pre-lab Assignment Before coming to lab: Read the lab thoroughly. Answer the pre-lab questions that appear at

More information

Hydrogen Peroxide Analysis

Hydrogen Peroxide Analysis elearning 2009 Introduction Hydrogen Peroxide Analysis Consumer Chemistry Publication No. 91253 Hydrogen peroxide is regarded as an environmentally friendly alternative to chlorine for water purification

More information

Experiment 13H THE REACTION OF RED FOOD COLOR WITH BLEACH 1

Experiment 13H THE REACTION OF RED FOOD COLOR WITH BLEACH 1 Experiment 13H FV 1/25/2011(2-run) THE REACTION OF RED FOOD COLOR WITH BLEACH 1 PROBLEM: Determine the rate law for the chemical reaction between FD&C Red Dye #3 and sodium hypochlorite. LEARNING OBJECTIVES:

More information

Target Mole Lab. Mole Relationships and the Balanced Equation. For each student group Hydrochloric acid solution, HCl, 3 M, 30 ml

Target Mole Lab. Mole Relationships and the Balanced Equation. For each student group Hydrochloric acid solution, HCl, 3 M, 30 ml elearning 2009 Introduction Target Mole Lab Mole Relationships and the Balanced Equation Publication No. A common chemical reaction used in chemistry class is zinc and hydrochloric In this lab, students

More information

Experiment 16 The Solution is Dilution

Experiment 16 The Solution is Dilution Experiment 16 The is Dilution OUTCOMES Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions. prepare a solution of known concentration. prepare a dilute

More information

Kinetics mg of p-nitrophenyl acetate, 65 ml of methanol, 13.9 mg of p-nitrophenol, 6.7 g of KCl.

Kinetics mg of p-nitrophenyl acetate, 65 ml of methanol, 13.9 mg of p-nitrophenol, 6.7 g of KCl. Kinetics In this experiment you will determine the order of a reaction with respect to the two reactants, p-nitrophenyl acetate and hydroxide. You will also determine the rate constant for this reaction.

More information

Distillation Experiment

Distillation Experiment Distillation Experiment CHM226 Background The distillation process is a very important technique used to separate compounds based on their boiling points. A substance will boil only when the vapor pressure

More information

Experiment 8 Synthesis of Aspirin

Experiment 8 Synthesis of Aspirin Experiment 8 Synthesis of Aspirin Aspirin is an effective analgesic (pain reliever), antipyretic (fever reducer) and anti-inflammatory agent and is one of the most widely used non-prescription drugs. The

More information

Names of students present in your group

Names of students present in your group Your name Names of students present in your group Lab Instructor Lab & Table Number SCI 265 INVESTIGATION HOUSEHOLD CHEMISTY Many of the households are acid or bases. Acids and bases are important compounds

More information

Experiment 8: Synthesis and Analysis of an Inorganic Compound CH3500: Inorganic Chemistry, Plymouth State University

Experiment 8: Synthesis and Analysis of an Inorganic Compound CH3500: Inorganic Chemistry, Plymouth State University Experiment 8: Synthesis and Analysis of an Inorganic Compound CH3500: Inorganic Chemistry, Plymouth State University Adapted from Johnson, R.C. "Convenient procedure for the preparation of potassium trioxalatoferrate(iii),"

More information

Continued on next page

Continued on next page 1 Breathalyzer Reaction Introduction A breathalyzer (breath analyzer) is a device for estimating blood alcohol content (BAC) from a breath sample. The invention of the breathalyzer provided law enforcement

More information

THE THERMODYNAMICS OF POTASSIUM NITRATE DISSOLVING IN WATER VERSION V121113

THE THERMODYNAMICS OF POTASSIUM NITRATE DISSOLVING IN WATER VERSION V121113 THE THERMODYNAMICS OF POTASSIUM NITRATE DISSOLVING IN WATER VERSION V121113 OBJECTIVE The ΔG, ΔH and ΔS of the potassium nitrate (KNO3) dissolving reaction will be determined by measuring the equilibrium

More information

Bellevue College CHEM& 121 Experiment: Stoichiometric Analysis of an Antacid 1

Bellevue College CHEM& 121 Experiment: Stoichiometric Analysis of an Antacid 1 Experiment: Stoichiometric Analysis of an Antacid 1 Introduction In this lab, you will use the concept of stoichiometry to solve two sequential problems. First, you will try to determine the products of

More information

Ascorbic Acid Titration of Vitamin C Tablets

Ascorbic Acid Titration of Vitamin C Tablets Ascorbic Acid Titration of Vitamin C Tablets Procedure Each Part of lab requires a separate data table. You might want to put each table on a separate page so you can leave room to show equations and calculations.

More information

Standard Operating Procedure for a Chemical Spill

Standard Operating Procedure for a Chemical Spill Standard Operating Procedure for a Chemical Spill Facility: Lab Director: Scope: ERC Clean Room C17 Engineering Research Complex Electrical and Computer Engineering Brian Wright 3234 Engineering 355-5233

More information

1 Spectrophotometric Analysis of Commercial Aspirin

1 Spectrophotometric Analysis of Commercial Aspirin 1 Spectrophotometric Analysis of Commercial Aspirin Name: _ Date: Lab Day/Time: Lab Partner: Objectives Learn about the absorption of light by molecules Learn the basic components of a spectrophotometer

More information

BCH 312. Biochemical Calculations [Practical] Nora Aljebrin

BCH 312. Biochemical Calculations [Practical] Nora Aljebrin + BCH 312 Biochemical Calculations [Practical] Nora Aljebrin Office: Building 5, 3 rd floor, 5T267 Website: www.fac.ksu.edu.sa/naljebrin E.mail: naljebrin@ksu.edu.sa + Mark Distribution Report [ 5 Marks

More information

Evaluation copy. Case File 9. A Killer Cup of Coffee? GlobalTech manager dies

Evaluation copy. Case File 9. A Killer Cup of Coffee? GlobalTech manager dies Case File 9 Killer Cup of Coffee: Using colorimetry to determine concentration of a poison Determine the concentration of cyanide in the solution. A Killer Cup of Coffee? SOUTH PAINTER, Tuesday: It was

More information

H 2 N NH 2. ethylenediamine (en) phenanthroline (phen) catechol. ethylenediaminetetraacetic acid (EDTA) Cobalt Complexes Spring 2005 NF Page 1 of 6

H 2 N NH 2. ethylenediamine (en) phenanthroline (phen) catechol. ethylenediaminetetraacetic acid (EDTA) Cobalt Complexes Spring 2005 NF Page 1 of 6 Synthesis and Characterization of Cobalt Complexes Adapted from Preparation and Analysis of Two Coordination Complexes of Cobalt, Carnegie Mellon University. For this experiment: 1. Complete the Prelab

More information

INTRODUCTION TO THE SPECTROMETER COLOR OF AN ACID-BASE INDICATOR

INTRODUCTION TO THE SPECTROMETER COLOR OF AN ACID-BASE INDICATOR INTRODUCTION TO THE SPECTROMETER COLOR OF AN ACID-BASE INDICATOR The main purpose of this experiment is to introduce you to one of the most fundamental and th important instruments in the chemical laboratory,

More information

Structure of caffeine: O CH 3 CH 3

Structure of caffeine: O CH 3 CH 3 1 Extraction of Caffeine Introduction Caffeine Caffeine occurs naturally in tea leaves and coffee beans. Cocoa beans, used to produce chocolate, contain a compound that is nearly identical in structure

More information

Partner: Jack 17 November 2011. Determination of the Molar Mass of Volatile Liquids

Partner: Jack 17 November 2011. Determination of the Molar Mass of Volatile Liquids Partner: Jack 17 November 2011 Determination of the Molar Mass of Volatile Liquids Purpose: The purpose of this experiment is to determine the molar mass of three volatile liquids. The liquid is vaporized

More information

Experiment #7: Esterification

Experiment #7: Esterification Experiment #7: Esterification Pre-lab: 1. Choose an ester to synthesize. Determine which alcohol and which carboxylic acid you will need to synthesize your ester. Write out the reaction for your specific

More information

Physical and Chemical Properties and Changes

Physical and Chemical Properties and Changes Physical and Chemical Properties and Changes An understanding of material things requires an understanding of the physical and chemical characteristics of matter. A few planned experiments can help you

More information

Wolfson Campus Natural Science Department. Chemical Laboratory. What did I spill? Student Safety Contract

Wolfson Campus Natural Science Department. Chemical Laboratory. What did I spill? Student Safety Contract Wolfson Campus Natural Science Department Chemical Laboratory What did I spill? Student Safety Contract April 2000 Wolfson Campus Student Safety Contract-Chemistry Laboratory The Chemistry Laboratory courses

More information

**Lab Notebook** Directly above or below the data table, label a space to record the exact concentrations of KI and Pb(NO 3) 2

**Lab Notebook** Directly above or below the data table, label a space to record the exact concentrations of KI and Pb(NO 3) 2 Limiting Reagent This laboratory experiment has a formal report. Consult the guidelines for writing a formal laboratory report prior to doing this laboratory experiment so that you know what will be expected

More information

Measurement Lab. Background Information. I. Transferring Chemicals in the Laboratory

Measurement Lab. Background Information. I. Transferring Chemicals in the Laboratory Purpose of the Experiment Practicing techniques for weighing objects and measuring the volumes will allow you to be more adept in the lab environment. Determining the number of significant figures that

More information

Experiment 12- Classification of Matter Experiment

Experiment 12- Classification of Matter Experiment Experiment 12- Classification of Matter Experiment Matter can be classified into two groups: mixtures and pure substances. Mixtures are the most common form of matter and consist of mixtures of pure substances.

More information

Using Paper Chromatography to Separate Ink Virgil A. Sestini

Using Paper Chromatography to Separate Ink Virgil A. Sestini SCIENCE EXPERIMENTS ON FILE Revised Edition 5.31-1 Using Paper Chromatography to Separate Ink Virgil A. Sestini Topic Paper chromatography Time 1 hour! Safety Please click on the safety icon to view the

More information

COMMON LABORATORY APPARATUS

COMMON LABORATORY APPARATUS COMMON LABORATORY APPARATUS Beakers are useful as a reaction container or to hold liquid or solid samples. They are also used to catch liquids from titrations and filtrates from filtering operations. Bunsen

More information

UNIVERSITY OF MINNESOTA DULUTH DEPARTMENT OF CHEMICAL ENGINEERING ChE 3211-4211 ABSORPTION OF CARBON DIOXIDE INTO WATER

UNIVERSITY OF MINNESOTA DULUTH DEPARTMENT OF CHEMICAL ENGINEERING ChE 3211-4211 ABSORPTION OF CARBON DIOXIDE INTO WATER UNIVERSITY OF MINNESOTA DULUTH DEPARTMENT OF CHEMICAL ENGINEERING ChE 3211-4211 ABSORPTION OF CARBON DIOXIDE INTO WATER OBJECTIVE The objective of this experiment is to determine the equilibrium line,

More information

2 To identify ions present in unknown solutions using separation methods.

2 To identify ions present in unknown solutions using separation methods. Qualitative Analysis PURPOSE To develop a separation scheme and confirmatory tests for Fe 3+, Ba 2+, and Ag + cations, and to use it to identify the ions in a sample of unknown composition. GOALS 1 To

More information

Volumetric Glassware

Volumetric Glassware There are several types of glassware used to measure the volume of a liquid accurately. These include graduated cylinders, volumetric flasks, burets, and pipets. Graduated cylinders and volumetric flasks

More information

#14 Experimenting with Copper(II) Solutions Patricia Schroeder, Johnson County Community College, Overland Park, KS 66210

#14 Experimenting with Copper(II) Solutions Patricia Schroeder, Johnson County Community College, Overland Park, KS 66210 #14 Experimenting with Copper(II) Solutions Patricia Schroeder, Johnson County Community College, Overland Park, KS 66210 INTRODUCTION To close the yellow note, click once to select it and then click the

More information

THREE CHEMICAL REACTIONS

THREE CHEMICAL REACTIONS THREE CHEMICAL REACTIONS 1 NOTE: You are required to view the podcast entitled Decanting and Suction Filtration before coming to lab this week. Go to http://podcast.montgomerycollege.edu/podcast.php?rcdid=172

More information

ENVR 1401 LAB EXERCISE Lab : Toxicity Testing and Determining the LC 50

ENVR 1401 LAB EXERCISE Lab : Toxicity Testing and Determining the LC 50 SAFETY CONCERNS: ENVR 1401 LAB EXERCISE Lab : Toxicity Testing and Determining the LC 50 Chemical splash goggles must be worn by everyone in the lab while chemicals are out and in use. Goggles and a sterilizing

More information

Experiment 1F-1 MEASUREMENTS IN THE CHEMISTRY LABORATORY I

Experiment 1F-1 MEASUREMENTS IN THE CHEMISTRY LABORATORY I Experiment 1F-1 MEASUREMENTS IN THE CHEMISTRY LABORATORY I FV 1-15-15 MATERIALS: room-temperature distilled water, 2 100 ml beakers, 150 ml beaker, plastic dropper, small plastic (for both weeks) bottle

More information

Acid Base Titrations

Acid Base Titrations Acid Base Titrations Introduction A common question chemists have to answer is how much of something is present in a sample or a product. If the product contains an acid or base, this question is usually

More information