L PS, M2EN K. , Con A N K. Acta Physiologica Si nica Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

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Bartholomew Simmons
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1 106, , 51 (1), Acta Physiologica Si nica 3 (, ) (, ) A (Con A) (N K cell), (Naloxone, NL X) : (1) 1 ( IL21) ( ) (lipopolysaccharide, L PS, 50 ng/ 1 l) (2) (M2EN K, : 10 g/ l) 1 l, L PS (3) (10 g/ 1 l) (4), : ; ; ; : R97716, R322181, R446163, [1, ],, M2EN K, 1 ( IL2 1 ), IL ( HPA [2,3 ) ], Wistar,, g, L PS, M2EN K Sigma 10 % 3 ml/ kg, 1 l : ; ; ; ; : M2EN K 10 g/ l, L PS 50 ng/ l, 10 g/ l 8 10 min, 30 min,,, Con A N K t (No )

R322181, R446163, [1, ],, M2EN K, 1 ( IL2 1 ), IL21 2 2 ( HPA [2,3 ) ], Wistar,, 200 250 g, L PS, M2EN K Sigma 10 % 3 ml/ kg,

2 1 : 107 Con A 30 min,,,, / ml, 96,, Con A 5 g/ ml Con A 10 % l 10 % % CO h, M TT Con A [2, 570 nm OD, ], Con A, Con A OD N K, / ml 95 % Yac21 Yac21, Hank s 10 % RPM I 1640, / ml,, 95 % LDH N K, 3, 1 96, 5 % CO h 10 % l,, 96, 32, LDH, 3 min, 1 % 30 l, (570 nm) OD OD - OD N K = OD - OD 100 % 1 N K Table 1 Detecting procedure of N K cells activity Well Yac21 cells Effective cells 1 % NP 40 New bovine serum Test Native release Maximum release 11 Con A, Con A (OD 570nm ) ( x s x,, n = 29) ; 50 ng, ( n = 29),, ( P < 0105), L PS L PS ( n = 29 ), L PS ( P < 0101),, L PS( 2, 4 ) ( n = 33), L PS ( 2, 3 ) ( P < 0101), 21 Con A, 10 l, 30 min Con A (OD 570nm ) ( n = 20),

cells activity Well Yac21 cells Effective cells 1 % NP 40 New bovine serum + 1640 Test Native release Maximum release 11 Con A, Con A (OD 570nm ) 01035 01013 ( x s x,, n = 29) ; 50 ng, 01024 01009

3 ( n = 20), ( P < 0105) 2 Con A Table 2 (OD 570nm ) Proliferation values of splenic lymophocytes stimulated by Con A (OD 570nm ) 3 N K Table 3 Activity of N K cells( %) Group Drugs Proliferation values 1 Saline L PS M2EN K + L PS NLX+ M2ENK+LPS P < 0105 compared with NS control group ; 3 3 P < 0101 compared with LPS group ; P < 0101 compared with M2 ENK+ LPS group ; P > 0105 between 4 and 2 groups. Group Drugs Activity of NK cells / % 1 Saline L PS M2EN K + L PS NLX+ M2ENK+LPS P < 0,01 compared with NS control group ; 3 3 P < 0101 compared with LPS group ; P < 0101 compared with M2 ENK+ LPS group ; P > 0105 between 4 and 2 groups. 31 NK, N K ( ) % ( n = 18) 50 ng, N K ( ) % ( n = 18),, ( P < 0101), L PS N K ; L PS ( ) % ( n = 18), L PS ( P < 0101), L PS N K ( 3, 4 ), L PS ( ) % ( n = 18), L PS ( 3, 3 ), ( P < 0101), L PS ( P > 0105), L PS,, Con A, [1,4 ] HPA, ACTH [5 ],,,, 1,2 2 2, L TP, L TP, 1,2,3 [6 ] [7 9 ],,,,,,, L PS 1 ( IL21) IL21, IL21, IL21 HPA, [2,10,11 ]

LPS group ; 3 3 3 P < 0101 compared with M2 ENK+ LPS group ; P > 0105 between 4 and 2 groups.

4 1 : 109, Con A N K, N K,,, T2,,,,,,,,, 3 3 3, [1 ] Sheela DR, Namasicayam A, Prabhakaran K. Modulation of nonspecific immunity by hippocampal stimula2 tion. J Neuroim m unol, 1993, 42 : [2 ] Wang AJ ( ), Yang YZ ( ), Wu YM ( ), et al. Effect of intrahippocampal microin2 jection of cellular immune function and brain IL21 gene experssion in rat. Acta Physiol Sin ( ), 1996, 48 : (in Chinese with English abstract). [ 3 ] Wu YM ( ), Gao N ( ), Xie H ( ), et al. Influence of enkephaline on interleukin21 gene expression in cultured hippocampal gliacytes of neonatal rat. Chin J Pharm acol Toxicol ( ), 1996, 10 : (in Chinese with English abstract). [4 ] Brooks WH, Cross RJ, Roszman TL, et al. Neuroimmunomodulation : neural anatomical basis for impair2 ment and facilitation. A nn Neurol,1982, 12 : [5 ] Reul J MHM, de Kloet ER. Two receptors systems for corticosterone in rat brain : Microdistribution and differential occupation. Endocrinology, 1985, 117 : [6 ] Rothwell NJ, Hopkins SJ. Cytokines and the nervous system : actions and mecanisms of action. TINS, 1995, 18 : [ 7 ] Miller GC, Murgo AJ, Plotnikoff NP. Eekephalins2enhancement of active T2cell rosettes from normal volun2 teers. Clin Im m unol Im m unopathol, 1984, 31 : [8 ] Plotnikoff NP, Murgo AJ, Miller GC, et al. Enkephalin : immunomodulators. Fed Proc, 1985, 44 : [9 ] Shen Y ( ), Zhang LM ( ), Jiang J W ( ), et al. Modulation of mouse splenic lympho2 cyte proliferation by 2endorphin via opiate receptor. S hanghai J Im m unol ( ), 1992, 12 : (in Chinese with English abstract). [10 ] Blalock J E. The syntax of immune2neuroendocrine communication. Im m unol Today, 1994, 15 : [11 ] Payne LC, Weigent DA, Blalock J E. Induction of pituitary sensitivity to interleukin21 : a new function for corticotropin2releasing hormone. Biochem Biophys Res Com m un, 1994, 198 :

Acta Physiol Sin ( ), 1996, 48 : 348 354 (in Chinese with English abstract). [ 3 ] Wu YM ( ), Gao N ( ), Xie H ( ), et al.

5 110 Acta Physiologica Sinica Feb. 1999, 51 (1), OPIOID RECEPTOR MED IATED MOD ULATION OF INTRA HIPPOCAMPAL ENKEP HAL IN IND UCED CELL ULAR IMMUNE FUNCTION 3 GAO ( Depart ment of Physiology, Capital U niversity of Medical Sciences, Beijing ) WAN G A2J IN G, YAN G YU2ZHEN, HU MOU2XIAN, XIE HON G ( Reserch Center f or Ex perimental Medicine, Tongji Medical U niversity, W uhan ) NA ABSTRACT In the present work, the effect of intrahippocampal microinjection of opioid re2 ceptor antagonist naloxone on t he enhancement of cellular immune responses induced by enkep halin was studied in rat. The results showed t hat (1) t he proliferation activi2 ty of splenic lymp hocytes stimulated by Con A and natural killer ( N K) cell activity were decreased with microinjection of 1 l lipopolysaccharide (L PS, 50 ng/ l) into bilateral hippocampus ; (2) t he decrease of cellular immune responses induced by L PS could be inhibited by a preceding intrahippocampal injection of 1 l met2enkep halin (10 g/ 1 l) ; (3) t he enhancement of cellular immune responses induced by met2 enkephalin could be blocked by an opioid receptor antagonist naloxon (10 g/ l) ; and (4) cellular immune responses were also inhibited when naloxon was injected intrahip2 pocampally alone. The above results suggest t hat t he enhancement of cellular immune responses induced by enkep halin was mediated by opioid receptors in hippocampus. Key words : neuroimmunomodulation ; hippocampus ; enkep halin ; opioid receptor 3 Project Supported by the National Natural Science Foundation of China (No )

Sciences, Beijing 100054) WAN G A2J IN G, YAN G YU2ZHEN, HU MOU2XIAN, XIE HON G ( Reserch Center f or Ex perimental Medicine, Tongji Medical U niversity, W uhan 430030) NA ABSTRACT In the present

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