Product Datasheet. DARC Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 5

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1 Product Datasheet DARC Antibody NB Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 5/19/2016 v.20.1

2 NB DARC Antibody Product Information Unit Size Concentration Storage Clonality Preservative Purity Buffer Target Molecular Weight 0.1 mg 0.5 mg/ml Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.02% Sodium Azide Immunogen affinity purified Tris saline, ph 7.3 with 0.5% BSA. 36 kda Page 1 of 4 v.20.1 Updated 5/19/2016 Product Description Host Goat Gene ID 2532 Gene Symbol Species Specificity/Sensitivity DARC Human This antibody is expected to recognize both reported isoforms (NP_ ; NP_ ). Immunogen Peptide with sequence HRAELSPSTENSSQLDFED-C corresponding to N- Terminus according to NP_ , NP_ Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA Western Blot ug/ml, Immunohistochemistry 3 ug/ml, Immunohistochemistry-Paraffin 3 ug/ml, Peptide ELISA Detection limit 1:16000 FLOW: An anonymous customer has reported successful staining of HEK293S- TetR transfected with DARC receptor at a dilution of 1/1000. WB: Approx. 35 kda band observed in human kidney and human liver lysates (calculated MW of 35.6 kda band according to NP_ ). IHC-P: Human kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. IHC successful usage on human Lung showing endothelial staining. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

3 Images Western Blot: DARC Antibody [NB ] - Human Liver lysate Page 2 of 4 v.20.1 Updated 5/19/2016 Immunohistochemistry: DARC Antibody [NB ] - Human Kidney. Publications Davis MB, Walens A, Hire R et al. Distinct Transcript Isoforms of the Atypical Chemokine Receptor 1 (ACKR1)/Duffy Antigen Receptor for Chemokines (DARC) Gene Are Expressed in Lymphoblasts and Altered Isoform Levels Are Associated with Genetic Ancestry and the Duffy-Null Allele. PLoS One 2015 [PMID: ] (IHC) Yu KD, Wang X, Yang C et al. Host genotype and tumor phenotype of chemokine decoy receptors integrally affect breast cancer relapse. Oncotarget 2015 Sep 22 [PMID: ] Bruhl H, Vielhauer V, Weiss M et al. Expression of DARC, CXCR3 and CCR5 in giant cell arteritis. Rheumatology (Oxford) 2005 Mar [PMID: ] Zeng XH, Ou ZL, Yu KD et al. Absence of multiple atypical chemokine binders (ACBs) and the presence of VEGF and MMP-9 predict axillary lymph node metastasis in early breast carcinomas Med. Oncol Sep 01 [PMID: ] Zeng XH, Ou ZL, Yu KD, Feng LY, Yin WJ, Li J, Shen ZZ, Shao ZM. Coexpression of atypical chemokine binders (ACBs) in breast cancer predicts better outcomes. Breast Cancer Res Treat;125(3): Feb. [PMID: ] (IHC, Human)

4 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB HAF109 HAF017 H P01-10ug Human Liver Whole Tissue Lysate (Whole Normal) Donkey anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-goat IgG Secondary Antibody [HRP (Horseradish Peroxidase)] DARC Recombinant Protein

5 Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

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