Conversion of Agilent EI GC/MSD Systems To Hydrogen Carrier Gas. Agilent Technologies

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Conversion of Agilent EI GC/MSD Systems To Hydrogen Carrier Gas. Agilent Technologies"

Transcription

1 Conversion of Agilent EI GC/MSD Systems To Hydrogen Carrier Gas Agilent Technologies 1

2 Introduction: Converting From He To H 2 Many GC/MS users are considering changing from helium to hydrogen carrier gas due to price/availability problems with helium. This talk describes the steps recommended for converting EI GC/MS methods. It is important to recognize the differences with using hydrogen carrier. Time should be allotted for adapting the method, optimization, and resolving potential problems. Areas that will need attention include: choice of supply of H 2 Remove/bypass carrier gas filter (may be re-installed later if needed) GC/MSD hardware changes choosing new chromatographic conditions a 2-5 fold reduction in signal-to-noise ratio changes in spectra and abundance ratios for some compounds activity and reactivity with some analytes Page 2

3 Introduction: Converting From He To H 2 Methods that will generally require less optimization include analytes that: are durable compounds are at higher concentrations are analyzed with split injections are derivatized Methods that will generally require more optimization include analytes that: are fragile compounds are at trace concentrations Allow time for necessary updates to SOPs and validation. Page 3

4 4 H 2 Safety

5 Letter From Agilent Safety Engineer. Describes H 2 Safety Features of Agilent GCs 5

6 Designed for Reliability H 2 Safety Safety Shutdown When gas pressure set points are not met, the valve and heater are shut off to prevent explosion Flow Limiting Frit If valve fails in open position, inlet frit limits the flow Oven ON/OFF Sequence Fan purges the oven before turning on heater to remove any collected H 2 Explosion Test GC designed to contain parts in case of explosion Page 6

7 Safety First: Read This Before You Start G Page 7

8 Source of H 2 Carrier and Plumbing 8

9 Source of Hydrogen: Use A Hydrogen Generator Higher initial expense than cylinders, much lower cost over time Very clean H 2, > % available More consistant purity Safety considerations H 2 is only generated at needed pressure (like 40 psig) Flow is limited (like 250 ml/min) Auto-shutdown if setpoint pressure cannot be maintained Minimal stored gas (like 50 ml at 40 psig) of H 2 at any one time Make sure to buy a good one with a low spec for water and oxygen 9

10 10 This is an example of the type of H 2 generator to use. Look for generators with the > % specification and low individual specs on water and oxygen.

11 11

12 Plumbing The Instrument Chromatographic quality stainless steel tubing is often recommended for H 2 plumbing and is probably the best choice if available. Users may have to follow local codes or internal company guidelines. We have also used new 1/8 th copper that has been cleaned for GC use. Dirty tubing will cause huge contamination problems, as H 2 appears to carry dirt out of metal more than He does. Don t use really old copper tubing, as it becomes brittle and can break. Note that MSD leak checks will not always find big outgoing leaks. Leak check when complete with electronic leak detector. When plumbing a H 2 generator, start out with no traps. Only add traps if needed. Make sure the water and oxygen levels are low enough. 12 Confidentiality Label September 6, 2012

13 Split Vent and Septum Purge Vent Should Be Connected to Exhaust 13

14 MS Components Needed Before Conversion: Magnet and Drawout Lens 14

15 Check The Magnet In The 5975 If the magnet in the 5975 does NOT have a serial number along the left edge (as viewed through the source window), it is suitable for use with Helium, but not Hydrogen. You should contact your Agilent service engineer to change it to a Helium and Hydrogen compatible magnet before converting the system to H 2 carrier. Note: all 5973 magnets are compatible with both He and H2 15

16 Replace The Standard 3 mm Drawout Lens With The Optional 6 mm When Using H 2 Carrier G mm Drawout Plate, Inert Standard 3 mm Drawout Plate, Inert 16 Confidentiality Label September 6, 2012

17 Choosing A Column And Conditions 17 Confidentiality Label September 6, 2012

18 MSD Pumping Capacity For H 2 Is Less Than For He This limits column choice Complete info on H 2 pumping specs are not yet available These are approximate values for the Max flow of H 2 to maintain reasonable source pressure: Performance turbo: 2 ml/min Standard turbo: 1 ml/min Diffusion pump: 0.75 ml/min Pressure pulsing: turbos <= 3 ml/min, diffusion <= 2.5 ml/min It is very helpful to have an ion gauge on the MS to monitor the vacuum vs column flow. Try to avoid flows that produce pressures higher than 5 x 10-5 torr. You can get useful data above this pressure, but performance starts to degrade rapidly 18

19 Guidelines For Choosing Column Dimensions And Flows For Method Converted From He To H 2 Determine max flow of H 2 into MS that will give source pressure of 5 x 10-5 torr or less source pressure. This is your max column flow. Choose column dimensions at initial oven temp of method to give: A flow <= max column flow for vacuum pump A flow >= min column flow for efficiency An inlet pressure of at least 5 psig Keeping a temperature ramp of the same number of C/void time will give similar elution order. Use the method translator for this. These are only approximate guidelines. Sometimes you have to deviate from them 19

20 Van Deemter Curves If the flow of He or H 2 is too low, you loose efficiency MUCH FASTER than if it is too high. This is why operating at or above minimum linear velocity is important. Use 35 cm/sec as minimum for H 2 20

21 Column Choice When in doubt (or a hurry), use: Use 20m x 180 um column and set constant flow at ml/min. Try to get a linear velocity of at least 35 cm/sec Try to use the same phase and phase ratio to get similar elution order. Smaller bore columns have lower capacity. You may have overloading on high level cals or samples You may need to use pulsed injection to load sample into small bore column with low flow rate. The flow range for your H 2 setup is limited on the low side by the flow to get 35 cm/sec and on the high side by the vacuum pump capacity 21

22 Using The Flow Calculator. Set Linear Velocity to 35 For H 2 Carrier. If InletPressure Too Low, Raise Flow Inlet pressure is too low Set inlet pressure to 5 psig 22

23 Example: 30m x 0.25 mm id x 0.25 um Checkout Column With Perf Turbo Meets flow requirements for pump and column efficiency with 2 x speed gain 23

24 Example: 20m x 0.18 mm id x 0.18 um Column With Perf Turbo and Std Turbo Meets flow requirements for std turbo pump. Flow here is chosen to give a 2.5 x speed gain. 24

25 Status Of Calculators For Column Selection HP FlowCalc 2.0 and HP Method Translator used in earlier slides DO NOT WORK on Win 7 Newer versions of these can also be found in: GC/GCMS Instrument Utilities Kit. This CD ships with every Agilent GC, GC/MS, and autosampler. It also has manuals for GC and a firmware updating tool. 25

26 Initial Startup With Hydrogen 26

27 Initial Problems With Switching GC/MS Methods to H 2 High background that looks like hydrocarbons Reduced signal to noise (worse MDL) Significant tailing for many compounds Page 27

28 Switch to H 2. Early problem- High Background You will see this throughout work with H 2. It will get much smaller with time and use. It used to takes days or weeks for this to fall to acceptable levels. Agilent has a new conditioning protocol to clean up the background much faster (see later slide). Page 28

29 Tox Checkout Sample Abundance Time--> Immediately after conversion to H 2, peakshapes on TIC are poor and tailing badly. 20m x 0.18mm id x 0.18 um DB-5MSUI Hydrogen Abundance Time--> Helium Page 29

30 GC/MS Toxicology Checkout Mixture Amphetamine 2 Phentermine 3 Methamphetamine 4 Nicotine 5 Methylenedioxyamphetamine(MDA) 6 Methylenedioxymethamphetamine(MDMA) 7 Methylenedioxyethylamphetamine 8 Meperidine 9 Phencyclidine 10 Methadone 11 Cocaine 12 SKF-525a (RTL Compound) 13 Oxazepam 9 14 Codeine 5 ng/ul each compound Lorazepam 16 Diazepam 17 Hydrocodone 18 Tetrahydrocannabinol 19 Oxycodone 20 Temazepam 21 Flunitrazepam 22 Diacetylmorphine 23 Nitrazepam 24 Clonazepam 25 Alprazolam 26 Verapamil 27 Strychnine 28 Trazodone

31 Tailing Peaks: There is More Going On Here Than Simple Tailing Cocaine Abundance Peak Spectrum Abundance Tail Spectrum Average of to min.: ToxCKO_H2_4.D\data.ms Average of to min.: ToxCKO_H2_4.D\data.ms Cocaine Not Cocaine m/z--> m/z--> Page 31

32 Main Ions Of Cocaine Do Not Tail. Tail Made Up of Other Ions Tail is different than peak. May be formed from catalytically shredded main component. This problem is reduced with time Cocaine Tail is not cocaine ions Page 32

33 Minimizing The Time For Chromatography and Background To Stabilize Have a good source of clean hydrogen like a > % purity hydrogen generator. Keep H 2 flow limited to value suitable for your pumping system Use 6 mm drawout lens After setup, purging and pumpdown: Set the source to max temp for your source (check to see what your s is) Reduce the EMV to <= 800V Leave the FILAMENT ON overnight. This cleans up background rapidly. Peakshape will be much better and background will be much lower in the morning. Lower the source temp to method value, retune, and run some samples It is a good idea to have an extra set of filaments on hand in case one burns out. This hasn t been a problem, but a good idea. Page 33

34 H 2 Method With 6 mm Drawout And Overnite Cleaned Source. Peakshape Much Better and Background is Lower. Abundance Time--> Page 34

35 Performance Expectations Signal-to-noise ratio is usually worse by about 2-5 x. This obviously varies from compound to compound While most spectra remain same, there are always exceptions. Users should check the reference spectra for important targets to make sure they have not changed Same comment for target/qualifier ratios Some compounds may disappear at low levels. Examples include: some nitro and oxygen containing compounds (alcohols, aldehydes, ketones) like those found in flavor samples 35

36 Other Considerations: H 2 is not an inert gas, and therefore inertness problems will still exist or be worse. Some things to consider: Use the lowest inlet temp that works (to reduce reactions with H 2 ) Use pulsed injection, especially with small bore columns Consider using an MMI in cold splitless mode for fragile compounds Using a deactivated S/SL weldments might help (but don t ever scrub it) PN:G (bottom) PN:G (top) Avoid using methylene chloride as a solvent (especially wet). At higher inlet temps (like 300C), HCl is formed and causes problems. It can remove the gold from the seal and corrode the weldment. If DCM must be used, use lowest inlet temp and maybe a deactivated S/SL weldment (or MMI) Also avoid carbon dissulfide as a solvent Use liners with a taper at bottom to minimize sample contact with gold seal Use Agilent Ultra Inert Liners. 36

37 Hydrogen Carrier Summary 1. Get a GOOD hydrogen generator and plumb it properly 2. Pick a column that meets the flow needs of method and MSD pump 3. Do magnet update (if necessary) and install 6 mm drawout lens 4. After purging and pumpdown, set source to max temp and quad to 200C. Leave filaments on all night. 5. Reset source temp, retune, and run samples. Run practice samples for a few days to let system stabilize

Addressing the World Helium Shortage For Gas Chromatography

Addressing the World Helium Shortage For Gas Chromatography Addressing the World Helium Shortage For Gas Chromatography Helium Conservation and Converting GC Methods to Nitrogen and Hydrogen Carrier Gas James D. McCurry, Ph.D. Senior Scientist Agilent Technologies

More information

Analysis of Drugs by using the Ultra Inert Inlet Liners with Wool

Analysis of Drugs by using the Ultra Inert Inlet Liners with Wool nalysis of Drugs by using the Ultra Inert Inlet Liners with Wool pplication Note Forensic Toxicology uthors Limian Zhao, ruce Quimby gilent Technologies, Inc. 280 Centerville Road Wilmington, DE 19808

More information

The Use of Hydrogen Carrier Gas for GC/MS

The Use of Hydrogen Carrier Gas for GC/MS Technical Note The Use of Hydrogen Carrier Gas for GC/MS Gas Chromatography/ Mass Spectrometry Highlights Guidelines that can mitigate dangers and leverage benefits of using hydrogen Key safety factors

More information

Hydrogen Carrier Gas for Total Petroleum Hydrocarbon. By Ed Connor, GC Product Specialist, Peak Scientific Instruments Ltd

Hydrogen Carrier Gas for Total Petroleum Hydrocarbon. By Ed Connor, GC Product Specialist, Peak Scientific Instruments Ltd Hydrogen Carrier Gas for Total Petroleum Hydrocarbon By Ed Connor, GC Product Specialist, Peak Scientific Instruments Ltd Many labs are facing helium supply difficulties and rapid price increases as a

More information

23 The Thermal Conductivity Detector

23 The Thermal Conductivity Detector 23 The Thermal Conductivity Detector General Information TCD pneumatics Conditions that prevent the detector from operating Filament passivation Carrier, reference, and makeup gas Negative polarity Analyzing

More information

Tuning & Mass Calibration

Tuning & Mass Calibration Tuning & Mass Calibration 1 1 The Sample List Sample List Name Project Name 2 The sample list is the top level screen in the TurboMass Gold Software. Data storage is set up in PROJECT files and within

More information

A Comparison of GC-Inlets for Simulated Distillation Analyses

A Comparison of GC-Inlets for Simulated Distillation Analyses A Comparison of GC-Inlets for Simulated Distillation Analyses David Grudoski wemeasureit Albany,CA 1 A Comparison of GC-Inlets for Simulated Distillation Analyses Abstract: 2013 Gulf Coast Conference,Galveston

More information

Injection Techniques for Capillary GC

Injection Techniques for Capillary GC Injection Techniques for Capillary GC Updated: 28-May-2014 Agenda Overview Practical Considerations Split Injection Splitless Injection On-Column Injection Direct Injection sigma-aldrich.com/analytical

More information

5973 inert Mass Selective Detector

5973 inert Mass Selective Detector 5973 inert Mass Selective Detector Hardware Manual Agilent Technologies 5973 inert Mass Selective Detector Hardware Manual Notices Agilent Technologies, Inc. 2003 No part of this manual may be reproduced

More information

Waters Corporation. Waters 2690/5 USER & TROUBLESHOOTING GUIDE

Waters Corporation. Waters 2690/5 USER & TROUBLESHOOTING GUIDE Waters Corporation Waters 2690/5 USER & TROUBLESHOOTING GUIDE Contents 2690/5 Theory Setup procedures. Troubleshooting the 2690/5 User maintenance of the 2690/5 Spare Parts 2 2690/5 Theory 2690/5 Solvent

More information

We will start with establishing a logical approach to troubleshooting, how to organize your thoughts, if you will. Next, I will focus on some specific

We will start with establishing a logical approach to troubleshooting, how to organize your thoughts, if you will. Next, I will focus on some specific Hello everyone, and thank you for attending this webinar today. GC troubleshooting is a very broad topic, and we could easily devote an entire day to talking about it. In this webinar, I will be focusing

More information

DETECTORS Nitrogen/Phophorus Detector - NPD. Overview

DETECTORS Nitrogen/Phophorus Detector - NPD. Overview Overview The SRI Nitrogen-Phosphorus Detector (NPD) has a linear response selective to organic compounds containing nitrogen and/or phosphorus. The NPD also responds to normal hydrocarbons, but approximately

More information

Hydrogen as an alternative to Helium for gas chromatography.

Hydrogen as an alternative to Helium for gas chromatography. Application Note Gas Chromatography. Application Note for Specialty Gases. as an alternative to for gas chromatography. Author: Peter Adam Linde AG, Linde Gas Division Seitnerstrasse 70, 82049 Pullach,

More information

The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes and Styrene in Olive Oil Using a Turbomatrix HS and a Clarus SQ 8 GC/MS

The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes and Styrene in Olive Oil Using a Turbomatrix HS and a Clarus SQ 8 GC/MS application Note Gas Chromatography/ Mass Spectrometry Author A. Tipler, Senior Scientist PerkinElmer, Inc. Shelton, CT 06484 USA The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes

More information

The Characterization of Perfume Fragrances Using GC/MS, Headspace Trap and Olfactory Port

The Characterization of Perfume Fragrances Using GC/MS, Headspace Trap and Olfactory Port APPLICATION NOTE Gas Chromatography/ Mass Spectrometry Authors: Andrew Tipler Sheila Eletto PerkinElmer, Inc. Shelton, CT The Characterization of Perfume Fragrances Using GC/MS, Headspace Trap and Olfactory

More information

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403 Gas Chromatograph Mass Spectrometer No. GCMS-1403 Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection Introduction Determination of Blood Alcohol Content (BAC) has been a standard

More information

Carrier Gas Selection for Capillary Gas Chromatography

Carrier Gas Selection for Capillary Gas Chromatography Carrier Gas Selection for Capillary Gas Chromatography Len Sidisky, Greg Baney, Katherine Stenerson, and James L. Desorcie Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA T411126 www.sigma-aldrich.com

More information

Application Note. Abstract. Author. Forensic and Toxicology

Application Note. Abstract. Author. Forensic and Toxicology An Application Kit for the Screening of Samples for Analytes of Forensic and Toxicological Interest using LC/QQQ MS/MS with a Dynamic MRM Transition Database Application Note Forensic and Toxicology Author

More information

Expectations for GC-MS Lab

Expectations for GC-MS Lab Expectations for GC-MS Lab Since this is the first year for GC-MS to be used in Dr. Lamp s CHEM 322, the lab experiment is somewhat unstructured. As you move through the two weeks, I expect that you will

More information

Hydrogen as an alternative to Helium for gas chromatography.

Hydrogen as an alternative to Helium for gas chromatography. HiQ Application Note Gas Chromatography Application Note for Specialty Gases. Hydrogen as an alternative to Helium for gas chromatography. Author Peter Adam, Linde AG, Linde Gases Division, Seitnerstrasse

More information

Background Information

Background Information 1 Gas Chromatography/Mass Spectroscopy (GC/MS/MS) Background Information Instructions for the Operation of the Varian CP-3800 Gas Chromatograph/ Varian Saturn 2200 GC/MS/MS See the Cary Eclipse Software

More information

Gas Chromatography Liner Selection Guide

Gas Chromatography Liner Selection Guide Gas Chromatography Liner Selection Guide Peter Morgan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20551 Key Words Liner, focus Abstract The liner serves an important function in allowing

More information

Fast Analysis of Natural Gas Using the Agilent 490 Micro GC Natural Gas Analyzer

Fast Analysis of Natural Gas Using the Agilent 490 Micro GC Natural Gas Analyzer Fast Analysis of Natural Gas Using the Agilent 90 Micro GC Natural Gas Analyzer Application Note Author Micro Gas Chromatography, Hydrocarbon Processing, Natural Gas Analysis Remko van Loon, Agilent Technologies,

More information

Analysis of Liquid Samples on the Agilent GC-MS

Analysis of Liquid Samples on the Agilent GC-MS Analysis of Liquid Samples on the Agilent GC-MS I. Sample Preparation A. Solvent selection. 1. Boiling point. Low boiling solvents (i.e. b.p. < 30 o C) may be problematic. High boiling solvents (b.p. >

More information

Agilent G1701DA MSD ChemStation

Agilent G1701DA MSD ChemStation Agilent G1701DA MSD ChemStation 5973 MSD / ChemStation Quick Reference Agilent Technologies What s New in Revision D.01.00 A new toolbar has been added to the Top view and an expanded set of toolbars have

More information

Monitoring Volatile Organic Compounds in Beer Production Using the Clarus SQ 8 GC/MS and TurboMatrix Headspace Trap Systems

Monitoring Volatile Organic Compounds in Beer Production Using the Clarus SQ 8 GC/MS and TurboMatrix Headspace Trap Systems application Note Gas Chromatography/ Mass Spectrometry Authors Lee Marotta Sr. Field Application Scientist Andrew Tipler Senior Scientist PerkinElmer, Inc. Shelton, CT 06484 USA Monitoring Volatile Organic

More information

Application. Gas Chromatography March Introduction

Application. Gas Chromatography March Introduction Large Volume Injection for Gas Chromatography Using a PTV Inlet Application Gas Chromatography March 1997 Authors Bill Wilson, Philip L. Wylie, and Matthew S. Klee Agilent Technologies, Inc. 285 Centerville

More information

Agilent 6890 Plus GC. Specifications: 6890 Plus Agilent GC, FID Single Capillary Inlet w/epc $15,500. Dimensions and Average Weight

Agilent 6890 Plus GC. Specifications: 6890 Plus Agilent GC, FID Single Capillary Inlet w/epc $15,500. Dimensions and Average Weight Agilent 6890 Plus GC >Home >Gas Chromatographs >Agilent 6890 Plus GC >More Info. $15,500 Specifications: 6890 Plus Agilent GC, FID Single Capillary Inlet w/epc Dimensions and Average Weight Height: 50

More information

Fractionation of Aliphatic and Aromatic Hydrocarbons in Petroleum Extracts

Fractionation of Aliphatic and Aromatic Hydrocarbons in Petroleum Extracts Fractionation of Aliphatic and Aromatic Hydrocarbons in Petroleum Extracts UCT Part Numbers XRSIHT15M25 ENVIRO-CLEAN EPH Fractionation 5mg/25mL (Gravity Flow) Capped at both ends to ensure silica integrity

More information

1.1 This test method covers the qualitative and quantitative determination of the content of benzene and toluene in hydrocarbon wax.

1.1 This test method covers the qualitative and quantitative determination of the content of benzene and toluene in hydrocarbon wax. Standard Method for Analysis of Benzene and Toluene Content in Hydrocarbon Waxes by Headspace Gas Chromatography EWF METHOD 002/03 (Version 1 Reviewed 2015) 1 Scope 1.1 This test method covers the qualitative

More information

Electrospray Ion Trap Mass Spectrometry. Introduction

Electrospray Ion Trap Mass Spectrometry. Introduction Electrospray Ion Source Electrospray Ion Trap Mass Spectrometry Introduction The key to using MS for solutions is the ability to transfer your analytes into the vacuum of the mass spectrometer as ionic

More information

THERMAL DESORPTION. Introduction and Principles. Focusing on Volatiles

THERMAL DESORPTION. Introduction and Principles. Focusing on Volatiles THERMAL DESORPTION Introduction and Principles Thermal Desorption Thermal desorption is a simple extension of the technique of gas chromatography (GC) It involves the use of heat and a flow of inert gas

More information

EPA Method RSK 175 Dissolved Gasses in Water Matrices using the Teledyne Tekmar HT3 Headspace Analyzer. HT3 Application Note.

EPA Method RSK 175 Dissolved Gasses in Water Matrices using the Teledyne Tekmar HT3 Headspace Analyzer. HT3 Application Note. EPA Method RSK 175 Dissolved Gasses in Water Matrices using the Teledyne Tekmar HT3 Headspace Analyzer HT3 Application Note Introduction The analysis of dissolved gases in ground water is important in

More information

GC Column Installation, Conditioning, Storage, and Maintenance

GC Column Installation, Conditioning, Storage, and Maintenance GC Column Installation, Conditioning, Storage, and Maintenance Peter Morgan, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20684 Key Words Column, installation, conditioning, storage,

More information

Agilent 7890B Gas Chromatograph

Agilent 7890B Gas Chromatograph Agilent 7890B Gas Chromatograph Operation Manual Agilent Technologies Notices Agilent Technologies, Inc. 2013 No part of this manual may be reproduced in any form or by any means (including electronic

More information

Comparison of BTEXS in Olive Oils by Static and Dynamic HT3 Headspace

Comparison of BTEXS in Olive Oils by Static and Dynamic HT3 Headspace Comparison of BTEXS in Olive Oils by Static and Dynamic HT3 Headspace Application Note Abstract The health benefits of consumption of olive oils as part of a healthy diet reaches back to the mid 1950 s.

More information

2 The Keyboard and Display

2 The Keyboard and Display 2 The Keyboard and Display The display The status board The keyboard Instant action keys [Start], [Stop], and [Prep Run] Function keys Short-cut keys [Temp], [Pres], [Flow], [Det Control], [Ramp #] [Temp],

More information

Extraction of Cannabinoids in Marijuana and Edibles by QuEChERS

Extraction of Cannabinoids in Marijuana and Edibles by QuEChERS Extraction of Cannabinoids in Marijuana and Edibles by QuEChERS UCT Part Numbers: ECQUEU750CT-MP - QuEChERS, Mylar packs containing 4 g magnesium sulfate, 1 g sodium chloride, 0.5 g sodium citrate dibasic

More information

Gas Chromatography Time of Flight Mass Spectrometry (GC-TOF MS) Northwestern University IMSERC Facilities Manual Version 1.0, A.

Gas Chromatography Time of Flight Mass Spectrometry (GC-TOF MS) Northwestern University IMSERC Facilities Manual Version 1.0, A. Gas Chromatography Time of Flight Mass Spectrometry (GC-TOF MS) Northwestern University IMSERC Facilities Manual Version 1.0, Gas Chromatography Time of Flight Mass Spectrometry (GC-TOF MS) is an electron

More information

The Electron Capture Detector (ECD)

The Electron Capture Detector (ECD) Theory of operation The ECD is based on the phenomenon that electronegative species can react with thermal electrons present to form negatively charged ions. The loss of such electrons is related to the

More information

Maintaining Your GC/MS System. Operate your Agilent GC/MS System with maximum efficiency. Diagnose tough MSD problems. Discover the best GC/MS columns

Maintaining Your GC/MS System. Operate your Agilent GC/MS System with maximum efficiency. Diagnose tough MSD problems. Discover the best GC/MS columns Diagnose tough MSD problems Discover the best GC/MS columns Access powerful support Maintaining Your GC/MS System Operate your Agilent GC/MS System with maximum efficiency. Maintaining Your GC/MS System

More information

Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine

Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine Comprehensive LC/MS Analysis of Illicit and Pain Management Drugs, Including Their Metabolites, in Urine Application Note Authors Peter JW Stone and Kevin McCann Agilent Technologies, Inc. Santa Clara,

More information

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Introduction The resolving power of chromatography combined with the sensitivity and selectivity

More information

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Simultaneous Quantitation of 4 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Xiang He and Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 76 Key Words TSQ Quantum Access

More information

CHROMOCTANE O N LINE ANALYZER

CHROMOCTANE O N LINE ANALYZER GECIL Process GT instruments CHROMOCTANE O N LINE ANALYZER ASTM D6733 DHA and RON and MON on line GC for Reformer unit Automatic Chromatographic system for analysis of naphta feeds and reforming gasolines

More information

XLR-11. [1-(5-fluoropentyl)-1H-indol-3-yl](2,2,3,3- tetramethylcyclopropyl)methanone. 5-Fluoro-UR-144. DEA Reference Material Collection.

XLR-11. [1-(5-fluoropentyl)-1H-indol-3-yl](2,2,3,3- tetramethylcyclopropyl)methanone. 5-Fluoro-UR-144. DEA Reference Material Collection. 1. GENERAL INFORMATION IUPAC Name: CAS #: 1364933-54-9 [1-(5-fluoropentyl)-1H-indol-3-yl](2,2,3,3- tetramethylcyclopropyl)methanone Synonyms: Source: Appearance: 5-Fluoro-UR-144 DEA Reference Material

More information

Clinical Chemistry (CHE221) Professor Hicks Lecture 12. Chromatography. chromatography. normal phase chromatography like a tug of war

Clinical Chemistry (CHE221) Professor Hicks Lecture 12. Chromatography. chromatography. normal phase chromatography like a tug of war Clinical Chemistry (CHE221) Professor Hicks Lecture 12 Chromatography chromatography technique to separate mixtures each peak a different substance stationery phase = material mixtures comes in contact

More information

The Determination of Benzene and Toluene in Finished Gasolines Containing Ethanol Using the PerkinElmer Clarus 680 GC with Swafer Technology

The Determination of Benzene and Toluene in Finished Gasolines Containing Ethanol Using the PerkinElmer Clarus 680 GC with Swafer Technology application Note Gas Chromatography Author A. Tipler PerkinElmer, Inc. Shelton, CT 06484 USA The Determination of and in Finished Gasolines Containing Using the PerkinElmer Clarus 680 GC with Swafer Technology

More information

Standard Test Method for Analysis of Hydrocarbon Waxes by Gas Chromatography EWF METHOD 001/03

Standard Test Method for Analysis of Hydrocarbon Waxes by Gas Chromatography EWF METHOD 001/03 1. Scope Standard Test Method for Analysis of Hydrocarbon Waxes by Gas Chromatography EWF METHOD 001/03 (Version 1 Reviewed 2015) 1. 1 This test method provides for the determination of the carbon number

More information

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications

Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications TECHNICAL NOTE Toxicology Making the Leap to LC/MS/MS: Enhancing and Accelerating Clinical Research and Forensic Toxicology Applications Introduction The resolving power of chromatography combined with

More information

GC Troubleshooting 10. How to use this Troubleshooting Help system... 10. GC Troubleshooting - Top Level... 10 Generic problem descriptions...

GC Troubleshooting 10. How to use this Troubleshooting Help system... 10. GC Troubleshooting - Top Level... 10 Generic problem descriptions... Table of Contents GC Troubleshooting 10 How to use this Troubleshooting Help system... 10 GC Troubleshooting - Top Level... 10 Generic problem descriptions... 11 Baseline related problems... 11 Peak related

More information

An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols.

An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. An Advanced Base Deactivated Capillary Column for analysis of Volatile amines Ammonia and Alcohols. Jaap de Zeeuw, Ron Stricek and Gary Stidsen Restek Corp Bellefonte, USA To analyze basic compounds at

More information

Split / Splitless Injection for Capillary GC

Split / Splitless Injection for Capillary GC Split / Splitless Injection for Capillary GC Speakers John V Hinshaw CHROMacademy GC Dean GC Connections editor Moderator Tony Taylor CHROMacademy Trainer Technical Director, Crawford Scientific Dave Walsh

More information

Gas Chromatograph GC Consumables. p/n: 220-94898-02

Gas Chromatograph GC Consumables. p/n: 220-94898-02 Gas Chromatograph GC Consumables p/n: 220-94898-02 Diag. # 1) Septum Nut 2) Needle Guide 3) Septum 4) Injection Port A Assy 5) O-ring for Glass Insert 6) Glass Insert 7) Gold Gasket 8) Capillary Adaptor

More information

Pesticide Analysis by Mass Spectrometry

Pesticide Analysis by Mass Spectrometry Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine

More information

Agilent 5977 Series MSD System

Agilent 5977 Series MSD System Agilent 5977 Series MSD System Concepts Guide Agilent Technologies Notices Agilent Technologies, Inc. 2013 No part of this manual may be reproduced in any form or by any means (including electronic storage

More information

Transformer Oil Gas Analysis

Transformer Oil Gas Analysis Get on the Analyzer FAST TRACK Transformer Oil Gas Analysis Helping customers protect the environment! 1 Why Analyzers Value to Customers Alleviates Resource Pressure Facilitates Configuration Use Advanced

More information

GenTech, Inc.

GenTech, Inc. GenTech Master GC offers a new approach to the world of gas chromatography by dramatically decreasing sample run times in a wide range of GC Applications. This GC is truly customizable to meet each user

More information

Agilent 7697A Headspace Sampler Data Sheet

Agilent 7697A Headspace Sampler Data Sheet Agilent 7697A Headspace Sampler Data Sheet Overview The Agilent 7697A Headspace Sampler is part of Agilent s line of gas chromtography products. The 7697A based on the architecture of the market-leading

More information

When your GC/MS is out of Breath

When your GC/MS is out of Breath When your GC/MS is out of Breath Helium and the use of alternative GC carrier gases Lee Marotta, Mike Baylerian, Alan Gallaspy, Andy Tipler, Miles Snow Outline Some background to Helium: where it comes

More information

Improved Method for the Analysis of 31 Drugs of Abuse in Oral Fluid samples using the Thomson extreme FV by LC-MS/MS

Improved Method for the Analysis of 31 Drugs of Abuse in Oral Fluid samples using the Thomson extreme FV by LC-MS/MS htslabs.com folks@htslabs.com 800 541.4792 760 757.8080 760 757.9367 Improved Method for the Analysis of 31 Drugs of Abuse in Oral Fluid samples using the Thomson extreme FV by LC-MS/MS Nadine Koenig 2,

More information

Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM. Technical Overview.

Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM. Technical Overview. Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM Technical Overview Introduction The 5975A and B series mass selective detectors (MSDs) provide

More information

Chemistry Instrumental Analysis Lecture 28. Chem 4631

Chemistry Instrumental Analysis Lecture 28. Chem 4631 Chemistry 4631 Instrumental Analysis Lecture 28 Gas Chromatography Detectors Gas Chromatography Monitors the column effluent and produces an electrical signal that is proportional to the amount of analyte

More information

Blood Alcohol Content Analysis using Nitrogen Carrier Gas

Blood Alcohol Content Analysis using Nitrogen Carrier Gas Blood Alcohol Content Analysis using Nitrogen Carrier Gas Alcohol consumption can seriously affect the ability of a driver to operate a vehicle and blood alcohol content (BAC) directly correlates with

More information

Results of Using Re-washed Vials and Closures

Results of Using Re-washed Vials and Closures Results of Using Rewashed Vials and Closures Loy Shick, Thermo Fisher Scientific, Rockwood, TN, USA Brian King, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20670 Key Words Autosampler,

More information

Micro GC Fusion Pre-Installation Checklist

Micro GC Fusion Pre-Installation Checklist Site Preparation Proper site preparation prior to installing is very important. Contact INFICON (www.inficon.com) with any questions regarding site preparation. NOTE: Checkboxes ( ) identify hardware that

More information

Solid Phase Micro Extraction of Tea Flavor Components Application Note

Solid Phase Micro Extraction of Tea Flavor Components Application Note Solid Phase Micro Extraction of Tea Flavor Components Application Note Food and Flavor Author Anne Jurek Applications Chemist EST Analytical Cincinnati, OH Abstract Tea flavors can vary from spicy to flowery

More information

PLOT Columns. Top: Bob Langford, GC Column Manufacturing Technician Bottom: Stephanie Sunner, Customer Service Representative

PLOT Columns. Top: Bob Langford, GC Column Manufacturing Technician Bottom: Stephanie Sunner, Customer Service Representative PLOT Columns Top: Bob Langford, GC Column Manufacturing Technician Bottom: Stephanie Sunner, Customer Service Representative PLOT Column Selection 93 Features & Benefits of Restek PLOT Columns Features

More information

GC/MS Analysis of Phthalates in Children s Products

GC/MS Analysis of Phthalates in Children s Products GC/MS Analysis of Phthalates in Children s Products Introduction Plastic is a well suited material for the manufacture of a wide range of products. Children s products, from plates and cups to mattress

More information

Clarus 580 Gas Chromatograph

Clarus 580 Gas Chromatograph S P E C I F I C A T I O N S Gas Chromatography Clarus 580 Gas Chromatograph The PerkinElmer Clarus 580 Gas Chromatograph (GC) is a fully automated gas chromatograph. The system offers the capabilities

More information

SCION SQ Series GC-MS

SCION SQ Series GC-MS SCION SQ Series GC-MS The Gas Chromatographers Detector SCION SQ TM GC-MS The SCION SQ series GC-MS systems combine innovative engineering with detailed cutomer requirements to produce a truly innovative

More information

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System Kent Johnson Fortes lab, Wilsonville Oregon List of drug of abuse candidates for

More information

Thermal Conductivity Detector

Thermal Conductivity Detector Thermal Conductivity Detector Troubleshooting Tips The thermal conductivity detector (TCD) detects the difference in thermal conductivity between column effluent flow (carrier gas + sample components)

More information

Clarus 500 Gas Chromatograph

Clarus 500 Gas Chromatograph Clarus 500 Gas Chromatograph The PerkinElmer Clarus 500 Gas Chromatograph (GC) is a fully automated gas chromatograph. The system offers the capabilities required for laboratories performing large numbers

More information

Overview. TCD detector oven setpoint (trimpot) Color-coded TCD filament leads are connected to the bridge terminal block

Overview. TCD detector oven setpoint (trimpot) Color-coded TCD filament leads are connected to the bridge terminal block Overview The Thermal Conductivity Detector (TCD) is the most universal detector available. Depending on the compound, the TCD responds with a detection range of 0.01% to 100% (100-1,000,000ppm). The SRI

More information

The CHROMacademy Essential Guide Understanding GC-MS Analysis Part 1

The CHROMacademy Essential Guide Understanding GC-MS Analysis Part 1 The CHROMacademy Essential Guide Understanding GC-MS Analysis Part 1 Speakers John Hinshaw GC Dept. Dean CHROMacademy Tony Taylor Technical Director Crawford Scientific Moderator M ( g ) e M ( g ) 2e Peter

More information

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)

More information

Series. Gas Chromatographs

Series. Gas Chromatographs Series 4060 Gas Chromatographs Series 4060 Overview The Teledyne Analytical Instruments (TAI) Series 4060 speciates gases of interest by combining well-accepted gas chromatography techniques with TAI s

More information

Determination of Polycyclic Aromatic Hydrocarbons (PAH) in Food with GC-MS/MS

Determination of Polycyclic Aromatic Hydrocarbons (PAH) in Food with GC-MS/MS Determination of Polycyclic Aromatic Hydrocarbons (PAH) in Food with GC-MS/MS Thorsten Bernsmann, Peter Fürst Chemical and Veterinary Analytical Institute Münsterland-Emscher-Lippe Joseph-König-Str. 40,

More information

Agilent GC Solutions. Advantages of Using Low Thermal Mass Oven (LTM) in Gas Chromatography CAG. Kelly Beard GC Applications October 17, 2012

Agilent GC Solutions. Advantages of Using Low Thermal Mass Oven (LTM) in Gas Chromatography CAG. Kelly Beard GC Applications October 17, 2012 Agilent GC Solutions Advantages of Using Low Thermal Mass Oven (LTM) in Gas Chromatography CAG Kelly Beard GC Applications October 17, 2012 LTM Series II Key Points Control by 7890 GC and GC, GC/MS SW

More information

13C NMR Spectroscopy

13C NMR Spectroscopy 13 C NMR Spectroscopy Introduction Nuclear magnetic resonance spectroscopy (NMR) is the most powerful tool available for structural determination. A nucleus with an odd number of protons, an odd number

More information

Solid Phase Micro Extraction of Flavor Compounds in Beer

Solid Phase Micro Extraction of Flavor Compounds in Beer Solid Phase Micro Extraction of Compounds in Beer ANNE JUREK Solid Phase Micro Extraction of Compounds in Beer Application Note Food/ Author Anne Jurek Applications Chemist EST Analytical Cincinnati, OH

More information

Hydrogen & Nitrogen as Alternative GC Carrier Gases SEPTEMBER/OCTOBER The Technology and Application of Industrial, Specialty and Medical Gases

Hydrogen & Nitrogen as Alternative GC Carrier Gases SEPTEMBER/OCTOBER The Technology and Application of Industrial, Specialty and Medical Gases SEPTEMBER/OCTOBER 2014 VOLUME 8 / ISSUE 5 The Technology and Application of Industrial, Specialty and Medical Gases Hydrogen & Nitrogen as Alternative GC Carrier Gases The Practical Impact of the Use of

More information

Gas Chromatography. Let s begin with an example problem: SPME head space analysis of pesticides in tea and follow-up analysis by high speed GC.

Gas Chromatography. Let s begin with an example problem: SPME head space analysis of pesticides in tea and follow-up analysis by high speed GC. Gas Chromatography Let s begin with an example problem: SPME head space analysis of pesticides in tea and follow-up analysis by high speed GC. Samples in 10mL sealed glass vials were placed in the MPS-2

More information

Hydrogen Isotope Separation Using Gas Chromatography

Hydrogen Isotope Separation Using Gas Chromatography 1 Hydrogen Isotope Separation Using Gas Chromatography Aaron Jo Victor Senior High School Advisors: Dr. Walter Shmayda, Neil Redden Dec 2012 2 Abstract A gas chromatograph (GC) system was commissioned

More information

Powerful Backup to Identification of Fatty Acid Methyl Esters

Powerful Backup to Identification of Fatty Acid Methyl Esters FAMEs GC/MS Library Fatty Acid Methyl Esters Library ver 1.0 For Shimadzu GCMS-QP2010 Series Powerful Backup to Identification of Fatty Acid Methyl Esters 240 Compounds: Mass Spectra and Retention Indices

More information

Fractional Distillation and Gas Chromatography

Fractional Distillation and Gas Chromatography Fractional Distillation and Gas Chromatography Background Distillation The previous lab used distillation to separate a mixture of hexane and toluene based on a difference in boiling points. Hexane boils

More information

Technical Overview. Author. Abstract. A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany

Technical Overview. Author. Abstract. A.G.Huesgen Agilent Technologies, Inc. Waldbronn, Germany Using the Agilent Instrument Control Framework to control the Agilent 1260 Infinity LC through Waters Empower software Instrument set up and performance Technical Overview Author A.G.Huesgen Agilent Technologies,

More information

Analyzing Small Molecules by EI and GC-MS. July 2014

Analyzing Small Molecules by EI and GC-MS. July 2014 Analyzing Small Molecules by EI and GC-MS July 2014 Samples Appropriate for GC-MS Volatile and semi-volatile organic compounds Rule of thumb,

More information

Lecture Chromo-3: Gas Chromatography. CHEM 5181 Fall 2004 Mass Spectrometry & Chromatography. Jessica Gilman and Prof. Jose-Luis Jimenez CU-Boulder

Lecture Chromo-3: Gas Chromatography. CHEM 5181 Fall 2004 Mass Spectrometry & Chromatography. Jessica Gilman and Prof. Jose-Luis Jimenez CU-Boulder Lecture Chromo-3: Gas Chromatography CHEM 5181 Fall 2004 Mass Spectrometry & Chromatography Jessica Gilman and Prof. Jose-Luis Jimenez CU-Boulder Outline Introduction Instrument overview Carrier gas Sample

More information

Appendix 5 Overview of requirements in English

Appendix 5 Overview of requirements in English Appendix 5 Overview of requirements in English This document is a translation of Appendix 4 (Bilag 4) section 2. This translation is meant as a service for the bidder and in case of any differences between

More information

Evaluation of Hydrogen as a Carrier Gas for Gas Chromatography / Mass Spectrometry. No. GCMS No. SSI-GCMS-1303

Evaluation of Hydrogen as a Carrier Gas for Gas Chromatography / Mass Spectrometry. No. GCMS No. SSI-GCMS-1303 Gas Chromatograph Mass Spectrometer No. GCMS-1303 Evaluation of Hydrogen as a Carrier Gas for Gas Chromatography / Mass Spectrometry Introduction Helium is the most commonly used carrier gas for gas chromatography/mass

More information

UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs

UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Application Note: 439 UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela UHPLC System MSQ Plus MS Detector Drugs

More information

Separation Science Application Note

Separation Science Application Note Separation Science Application Note Pegasus Characterization of Ballpoint Pen Inks by Solid-Phase Microextraction Gas Chromatography Time-of-Flight Mass Spectrometry Introduction Time-of-Flight Mass Spectrometry

More information

Opiates in Urine by SAMHSA GC/MS

Opiates in Urine by SAMHSA GC/MS application Note Gas Chromatography/ Mass Spectrometry Author Timothy D. Ruppel PerkinElmer, Inc. Shelton, CT 06484 USA Opiates in Urine by SAMHSA GC/MS Introduction The United States Department of Health

More information

Request for Quotes Goods/Trade Services RFQ for Gas Chromatograph Mass Spectrometer (GCMS) (PCS# 600000-1621-RFQ)

Request for Quotes Goods/Trade Services RFQ for Gas Chromatograph Mass Spectrometer (GCMS) (PCS# 600000-1621-RFQ) Request for Quotes Goods/Trade Services RFQ for Gas Chromatograph Mass Spectrometer (GCMS) Closing Date and Time: Wednesday, April 13, 2016 at 5:00 p.m. (Pacific Time) Contact: Aaron Galloway Fax: 541-888-3250

More information

Comparing US EPA and FDA Methods for 1,4-Dioxane in Consumer Products

Comparing US EPA and FDA Methods for 1,4-Dioxane in Consumer Products Comparing US EPA and FDA Methods for 1,4-Dioxane in Consumer Products Application Note Abstract 1,4-Dioxane routinely makes headlines when found in water or consumer products. A potential byproduct of

More information

Accessories for GC. Accessories for capillary columns. Ferrules for GC. Ordering information (packing unit 10 ferrules) 1/16" ferrules.

Accessories for GC. Accessories for capillary columns. Ferrules for GC. Ordering information (packing unit 10 ferrules) 1/16 ferrules. Ferrules for GC Graphite ferrules provide the highest temperature stability (up to 450 C). They are reusable, if handled with care. We also offer 1/16" graphite ferrules specially designed for Carlo Erba

More information