NCCR Structural biology Structure per period
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1 First funding period: Clusters Project title (per 2005) Leader (name) Surname Institute Institution Structural biology of membrane proteins Unversität Basel assemblies/molecular interactions Recombinat protein Plückthun Biochemie technologies Structure determination technologies Winkler Fritz Paul Scherrer Institut Villigen PSI Computational biomolecular sciences Individual Research Individual Research Individual Research Individual Research Individual Research Individual Research Individual Research Individual Research Individual Research Individual Research Folding of soluble proteins and membrane proteins in situe Evolutionary and computeraided protein engineering Microscopic analyses of membrane proteins in situ Membrane transporters and proteins from pathogenic organismus Protein-DANN interactions and supramolecular assemblies Protein crystallography of integral membrane proteins NMR spectroscopy of biological macromolecules Computational approaches to protein folding and drug design Computational biomolecular sciences Structural studes of cotranslational protein translocation Glockshuber Rudolf Plückthun Biochemie Grütter Markus Biochemie Basel Winkler Fritz Paul Scherrer Institut Villigen PSI Calfisch Amedeo Biochemie NCCR Structural biology 1
2 Clusters Project title (per 2005) Leader (name) Surname Institute Institution Individual Research Solution structure of a protein-rna complex that regulates alternativesplicing: PTB and nptb- RNA complexes Individual Research Structure and function of proteins involved in transmembrane chloride transport: chloride channels and chloride transporters Automation of the protein crystal beamline at the swiss light source New Isotope labeling strategies of protein and RNA for NMR spectroscopy investigations Production of archaeal N- linked glycoproteins in Escherichia coli Calorimetric analysis of protein folding and protein-ligand binding Synthesis of aminoacylated and isotope-labeled RNAsequences for structural investigations Dutzler Raimund Biochemie Schulze-Briese Clemens Paul Scherrer Institut Villigen PSI Aebi Markus Mikrobiologie Jelezarov Ilian Biochemie Pitsch Stefan Institute of Chemical Science and Engineering EPF Lausanne NCCR Structural biology 2
3 Second funding period: Clusters Project title (per 2009) Leader (name) Surname Institute Institution Structural biology of membrane proteins Unversität Basel assemblies/ molecular interactions Technologies Plückthun Biochemie Folding and function of and membrane proteins Glockshuber Rudolf Synthetic protein libraries Plückthun Biochemie Assessing the structure and dynamics of membrane proteins by electron and atomic force microscopy X-ray crystallography of and membrane transporters assemblies: structures of chromatin and associated multiprotein complexes Signalling and transport through biological membranes Solution NMR with soluble and membrane proteins, including supramolecular structures Computer simulation of membrane proteins, supramolecular complexes and macromolecular folding X-ray crystallography and electron microscopy of Grütter Markus Biochemie Unversität Basel Winkler Fritz Paul Scherrer Institut Villigen PSI NCCR Structural biology 3
4 Clusters Project title (per 2009) Leader (name) Surname Institute Institution Structure determination of protein-rna complexes involved in alternativesplicing by NMR spectroscopy Mechanisms of ion transport across membranes: The structural biology of ion channels and ion transporters Structure and mechanism of bacterial drug and antibiotic transporters Single molecule spectroscopy of cotranslational protein folding, structure, and dynamics Automation of protein crystallography beamlines for challenging projects at the swiss light source New isotope labeling strategies of protein and RNA for NMR spectroscopy investigations Calorimetric and biophysical analysis of protein-ligand binding and protein folding Technical development for automated and highthroughput data collection and 3D reconstruction in electron microscopy Dutzler Raimund Biochemie Locher Kaspar Schuler Ben Biochemie Schulze-Briese Clemens Paul Scherrer Institut Villigen PSI Jelezarov Ilian Biochemie Ishikawa Takashi NCCR Structural biology 4
5 Third funding period: Clusters Project title (per 2012) Leader (name) Surname Institute Institution Structural biology of Locher Kaspar membrane proteins assemblies/ molecular interactions Insitut für Technologies Plückthun Insitut für Biochemie Folding and function of and membrane proteins Directed evolution of eukaryotic membrane proteins X-ray crystallography of membrane proteins and the use of chaperones of crystallization Chromatin structure: Genome organization in three-dimensions Macromolecular assemblies involved in central cellular processes; mitochondrial protein synthesis and fatty acid synthesis The structural basis for transmembrane ion transport Structure and mechanism of multidrug transporters Structure determination of protein-rna complexes involved in alternativesplicing by NMR spectroscopy Glockshuber Rudolf Plückthun Biochemie Grütter Markus Biochemie Dutzler Raimund Biochemie Locher Kaspar NCCR Structural biology 5
6 Clusters Project title (per 2012) Leader (name) Surname Institute Institution Single molecule spectroscopy of cotranslational protein Schuler Ben Biochemie folding and membrane protein dynamics Computational biomolecular science Cell-free expression and segmental stable isotope labelling of proteins and RNAs for NMR studies High throughput expression lab High throughput crystallization facility of the NCCR Plückthun Biochemie Grütter Markus Biochemie NCCR Structural biology 6
7 NCCR Structural biology Participating research groups Participating groups Leader (name) Surname Institution Period Aebi Markus Calfisch Amedeo 1 Dutzler Raimund Basel 1+2 Glockshuber Rudolf Grütter Markus Ishikawa Takashi 2 Jelezarov Ilian 1+2 Locher Kaspar 2+3 Pitsch Stefan EPF Lausanne 1 Plückthun Schuler Ben 2+3 Schulze-Briese Clemens Villigen PSI Winkler Fritz Villigen PSI NCCR Structural biology 7
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