Basic Laboratory Safety (BLS V15-1) February 13-20, 2015 Campus Solna Preliminary schedule

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1 Basic Laboratory Safety (BLS V15-1) February 13-20, 2015 Campus Solna Preliminary schedule Day 1. Friday, February 13th Lecture hall: Wretlind, Widerströmska huset, Tomtebodavägen 18A Group rooms: WH-1, WH-2, WH-3, Amartya, Widerströmska huset, Tomtebodavägen 18A 09:00 Introduction to laboratory safety including rules and regulations * Roland Möllby, MTC and Ingela Djupedal, Environment and Security Unit, KI 10:20 Coffee 10:40 Introduction to risk assessments and handling instructions * Jenny Karlsson, Environment and Security Unit 11:05 Break 11:15 Group discussion I: Own safety issues work * Group rooms Roland Möllby, Jenny Karlsson, Ulrika Olsson and Ingela Djupedal, Environment and Security Unit, KI Note! Preparation necessary 12:00 Lunch 13:00 Chemical hazards work (I) Ulrika Olsson Note! Preparation necessary 14:15 Film: Laboratory accident 14:30 Break 14:50 Allergens in the laboratory -15:30 Ann-Sophie Merritt, IMM

2 Day 2. Monday, February 16th Time for self-studies s and assignment are performed in Ping-Pong Please note, deadline for web-tutorials and assignment below is 9 am, Tuesday February 17 th. s: Chemical hazards work (II) Introduction to the chemical database KLARA Cancer risks Responsible teacher: Ingela Djupedal Cytostatic drugs (introduction) Ventilated workplaces Responsible teacher: Ingela Djupedal Personal protection: Gloves Responsible teacher: Marie-Louise Lind, IMM Assignment: Evaluation and improvement of a chemical risk assessment To be discussed on Group discussion II, Tuesday, February 17th, 1pm

3 Day 3. Tuesday, February 17th Rooms for practical sessions and group practice:, Berzelius väg 3, 4 th floor; course laboratory, s väg 2; group room 08:55 Rotations of practical sessions and group practice meeting point: Tomtebodavägen 6, second floor - Please be in time! * i) Gloves, Marie-Louise Lind and Ingela Djupedal ii) Handling and, Ulrika Olsson iii) Contamination risks in the laboratory, Helene Fredlund & Mats Spångberg iv) Incidents- Spill, Jenny Karlsson Group 1 Group 2 Group 3 Group 4 9:00-9:35 i) Gloves ii) Handling & iii) Cont. risks iv) Incidents- 9:45-10:20 ii) Handling & i) Gloves iv) Incidents- iii) Cont. risks 10:40-11:15 iii) Cont. risks iv) Incidents- i) Gloves ii) Handling & 11:25-12:00 iv) Incidents- iii) Cont. risks ii) Handling & i) Gloves 12:00 Lunch Lecture hall: Marie, Widertrömska huset, Tomtebodavägen 18A Group rooms: Amartya, Mary, Ruth, WH-3, Widertrömska huset, Tomtebodavägen 18A 13:00 Group discussion II: Evaluation and improvement of a chemical risk assessment * Ulrika Olsson, Jenny Karlsson Note! Preparation necessary, see assignment day 2, Monday February 16th 14:00 Coffee 14:20 Conclusions and questions from lectures and web-tutorials on chemical hazards including cytostatic drugs and KLARA Ulrika Olsson 15:10 Break 15:20 Biosafety: Infection routes in the laboratory and laboratory acquired infections -16:10 Roland Möllby

4 Day 4. Wednesday, February 18th Computer rooms: Enter and Space, Berzelius väg 3 09:00 Computer assignment: waste management -11:00 Jenny Karlsson Assignment done by Thursday, February 19th to be discussed at 1pm (The assignment is mandatory and shall be performed in groups of two or three, However, presence at this session is not mandatory, the assignment may be performed elsewhere) Time for self-studies s are performed in Ping-Pong Please note, deadline for web-tutorials below is 9 am, Thursday February 19th s: Definitions of biological specimens Risk assessment- biological agents Laboratory biosafety- equipment and measures Responsible teacher: Roland Möllby Biosafety level 3 laboratory (BSL3) Disinfection Responsible teacher: Roland Möllby Blood handling (introduction) Responsible teacher: Roland Möllby Genetically modified microorganisms (introduction) Transport Responsible teacher: Ingela Djupedal Responsible teacher: Ann Rämme Strömberg Assignment: Responsible teacher: Ann Rämme Strömberg To be discussed, Thursday, February 19th

5 Day 5. Thursday, February 19th Lecture hall: Wretlindsalen Group rooms in the morning: Wretlind, Amartya, Parker, Ruth Group rooms in the afternoon: Amartya, WH-1, WH-2, WH-3, Ruth 9:00 Biosafety: The biosafety level 2 laboratory (BSL2) Roland Möllby 9:50 Coffee Biosecurity and dual use Roland Möllby 10:10 Conclusions and questions from web-tutorials on biosafety Roland Möllby and Jenny Karlsson 11:00 Break 11:10 Focus group Select one focus group that interests you, you may choose between: i) Blood handling Roland Möllby ii) Laboratory safety in animal facilities Helene Fredlund & Mats Spångberg, AFL, KM iii) GMM-retro- and lentiviral vectors Alf Grandien, MedH, KI 12:00 Lunch 13:00 Follow-up of computer assignment: waste management Ingela Djupedal Note! Preparation necessary, see assignment day 4, Wednesday, February 18th 13:30 Fire safety in the laboratory Christer Matonog, Environment and Security Unit, KI 13:55 Coffee 14:15 Group discussion III * -15:45 Roland Möllby, Jenny Karlsson, Ralf Morgenstern, Ann Rämme Strömberg Note! Preparation necessary, see assignment day 4, Wednesday, Feb 18th Group 1 Group 2 Group 3 Group 4 14:15-15:00 Follow up: Own Amartya Follow up: Own WH-2 Follow up: WH-3 Follow up: WH-1 15:00-15:45 Follow up: WH-3 Follow up: WH-1 Follow up: Own Amartya Follow up: Own WH-2

6 Day 6. Friday, February 20th Lecture hall: Wretlindssalen Computer room: Enter and Space 09:30 Examination: Course content Approximately 2hours * Wretlind -11:30 Roland Möllby and Ingela Djupedal 12:15 Examination: Risk assessment Computer room Enter and Space -14:00 Jenny Karlsson and Ulrika Olsson (Presence at this session is not mandatory, but it is mandatory to perform a risk assessment. Performed in groups of 2-3, see further instructions in Ping-Pong) Deadline for handling in risk assessment is March 4th, 2015 Lecture hall: Wretlindsalen Group rooms: WH-1, 2, 3, Amartya, Mary, Parker, Ruth (Widerströmska Huset) Computer rooms Enter, Space Course lab Meeting point Tuesday at 8:55, Tomtebodavägen 6, second floor

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