Sources of E. coli in Lake Martin, Alabama Brian Burnes

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9 Dick Bronson: Lake Watch of Lake Martin Can we identify sources of E.coli in Lake Martin?

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13 Procedure:. Collect E.coli from water and other possible sources. Assay antibiotic susceptibility patterns from E.coli 3. Compare patterns to identify E.coli sources

14 TE3 TE3 S S G G T3 T3 N3 N3 NA3 NA3 GM GM E5 E5 CIP5 CIP5 C3 C3 AM AM AMC3 AMC

15 Second Factor Sources of E. coli in Lake Martin - Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

16 Second Factor Sources of E. coli in Lake Martin - Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

17 Second Factor Sources of E. coli in Lake Martin - Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

18 Second Factor Sources of E. coli in Lake Martin - Cat/Dog Horse/Deer Human Geese Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

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21 Proposed depth profile sampling sites Proposed sampling sites

22 Second Factor Sources of E. coli in Lake Martin - Cat/Dog Horse/Deer Human Geese Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

23 Second Factor Sources of E. coli in Lake Martin - Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

24 Second Factor Sources of E. coli in Lake Martin - Cat/Dog Horse/Deer Human Geese Sample A. Human (Sugar Creek WWTP), n= B. Human (Cahaba waste treatment), n=5 C. Goose (Lake Martin, ), n=9 D. Goose (Lake Martin, 4), n=47 E. Goose (Cahaba waste treatment), n= F. Goose (Salmon River), n=85 G. Deer, n= H. Horse, n= I. Cat, n= J. Dog, n= K. Coley Creek Effluent, n= L. Wind Creek Lagoon Effluent (3 meters), n=5 M. Wind Creek Beach, n= N. E.coli reference strain, n= First Factor

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