Microbiology Sampling, processing and cultivation

Transcription

1 Microbiology Sampling, processing and cultivation

2 Sicherheit Petri Dishes... Sicherheit DishRack... Air Sampler Petri Dishes for Bacteriology Petri dishes from Sarstedt are made from highly transparent polystyrene and are heat-resistant to approximately 80 C for applications using hot agar. They are available in 35, 60, 92 and 150 mm diameter options. Their dimensional stability ensures optimal suitability for use in automated dish pourers as well as easy stacking. While versions with ventilation cams provide enhanced gas exchange, those without ventilation cams enable extended incubation times. Coloured dishes provide a variety of coding options. A Petri dish subdivided into two compartments is designed for parallel analyses under comparable conditions or the application of two different agar systems. In addition to round options, our range includes a square Petri dish for more efficient storage. Highly transparent polystyrene Stackable With and without ventilation cams Gamma irradiated options available Petri dishes for bacteriology From highly transparent polystyrene, heat-resistant to approx. 80 C ø/height Ventilation cams /bag /20 gamma-sterile with 100/ /16 without 480/ /16 with 480/ /16 gamma-sterile without 480/ /16 gamma-sterile with 480/ /15 gamma-sterile with / /10 gamma-sterile with /20 DishRack - The stacking solution for Petri dishes Convenient handling Stop knocking over stacks of dishes. Secure up to 88 dishes single-handedly with the DishRack. Each DishRack features retaining elements to accommodate 4 stacks of standard Ø 92/100 mm Petri dishes. Dishes can be removed from the rack at random, alleviating the laborious task of rearranging an entire stack. The DishRack is a valuable tool for multiuse at workstations, in incubators, or for sample storage. Durable and safe transport Elastic silicone strips securely hold dishes in the rack even if exposed to heavy shaking and movement during transport. The high-grade temperatureresistant plastic of the DishRack is largely resistant to most acidic and alkaline solutions. DishRack 50 for up to 52 plates Organization The DishRack is perfectly suited to accommodate, transport, incubate and store dishes in logistic systems. DishRack facilitates and helps to organise individual work processes. Our range includes 5 different colours and exchangeable writing strips for customisation. For example, the green DishRack might be used to store all urine samples and the yellow to store stool cultures. Convenient handling Secure transport Organization Available in 2 sizes and 5 colours Colour Height in cm neutral red yellow blue green 24 DishRack 80 for up to 88 plates Colour Height in cm neutral red yellow blue green 36 Coloured Petri dishes with ventilation cams ø/height Colour Petri dish, round, divided ø/height Ventilation cams /bag /16 with 480/20 Petri dish, square L x W x H 100 x 100 x 20 gamma-sterile Ventilation cams /bag /16 red 480/ /16 yellow 480/ /16 blue 480/ /16 green 480/20 /bag without 160/4 Air Sampler The GS 100 Air Sampler monitors ambient air quality for microbiological analyses in laboratories, hospitals and production facilities. The instrument operates according to the Anderson Air Sampler principle, aspirating ambient air through a jet plate while a micro processor controls and checks sampling. A thermal mass flow meter also precisely regulates the flow. While the air flow has been pre-set to 100 l/min, the inlet opening allows an aspiration speed of approximately 0.4 m/sec. Particles and microorganisms are transferred onto a standard Petri dish located underneath, without passing filters or nutrient solutions. Subsequently, the colonyforming units can grow directly on these dishes. Microbial count can then be carried out according to standard procedures. 100 l/min flow rate Autoclavable collection head Mains power pack or rechargeable battery Air Sampler Air Sampler GS 100, 230V Collection head GS Carrier case GS

3 Sicherheit POS PTS Automation Sicherheit... for Microbiology POS 720 Petri Dish Organization System PTS Petri Dish Transfer System The POS 720 and PTS represent important milestones in automation of microbiological laboratories with average to high sample throughput. In a fully automated process, more than 700 Petri dishes are labelled, stacked in sets and transported to individual workstations per hour. Labelling errors in microbiology laboratories are reduced and processing procedures are made simple and consistent, improving quality and competitiveness. Labour saving and easy to operate Reliable provision of all required Petri dishes Consistent barcode labelling and clear alphanumeric printing Dishes tracked throughout the process Additional labels for special media and agar available at the workstation POS 720 Petri Dish Organization System POS 720/PTS Power Mains supply Environment Adm. ambient temperature Max. relative humidity Dimensions Width x depth x height Weight Accessories Label printer Labels Capacity 230 V ± 10% / Hz / 400 VA +15 C to +35 C 80%, non-condensing 1,700 mm x 1,100 mm x 1,800 mm (height with assembled signal system) 200 kg, without Petri dishes Thermo direct printer with label dispenser and automatic spooling of excess paper Adhesive labels on rolls Supply: 10,000 pcs. / roll Format: 78 mm x 10 mm Material: Thermo Premium Top (other s are available on request) Adhesive: Permanent (other adhesives are available on request) The Petri Dish Transfer System transports the dish stacks pre-sorted by the POS 720 to the individual workstations. The free-standing system provides customised solutions and is adjustable in height within a defined range. Laboratory tables and benches can be conveniently positioned close to the PTS. A separating station with an integral scanner regulates the distance between the dish stacks. Exit points on the track ensure that stacks are correctly assigned to the relevant workstations. Dish stacks that cannot be identified by the scanner are transferred to an exit for error resolution. Signal lamp for status control and safe entry to POS 720 Layout example for PTS with three workstations (AP 1 AP 3) and exit for unidentifiable dishes PTS Petri Dish Transfer System PTS Power Mains supply Environment Adm. ambient temperature Max. relative humidity Dimensions Width x depth x height Weight AP 2 AP 1 AP V ± 10% / Hz / 322 VA +15 C to +35 C 80%, non-condensing Dependant upon customised design Dependant upon customised design Unidentified dishes Fehler Petri dishes Print Throughput All mm options (compatibility list available on request) Barcode and text symbols, customised layout Approximately 720 dishes/h Turntable with 15 magazines for 40 dishes each EDP link Network connection to laboratory LANRS232/V24 Culture media dishes are automatically Printer and applicator print and attach the labels to the dish base Separating station with scanner Sorted Labelled/printed Stacked by specimen type Transported to workstations Dish stacker creates one stack per specimen type Feed track adaptable to any room layout Dish with barcode and customised layout Dish stack on its way to the workstation WOUND 4 5

4 Sicherheit Inoculation... Loops Swabs Swabs Sicherheit... Inoculation Loops, Needles and Spreaders Sarstedt s inoculation loops, needles and spreaders for single use are easy to handle, provide enhanced safety, minimise the risk of cross contamination, and save time. Heat sterilising in a Bunsen burner flame is no longer necessary between two inoculation processes, eliminating the spread of pathogenic aerosols. Flexible inoculation loops facilitate smears or inoculation in liquid media. 1 and 10 μl loops are colour-coded for easy identification. Sarstedt s inoculation needle is ideal for the transfer of individual colonies while the inoculation spreader serves to spread larger volumes across culture media. Time-saving, particularly for extensive test series Easy to use Superior safety Gamma-irradiated Demeter pipette 1.1 ml with and without tip To produce dilutions for bacteriological tests, e.g. in food labs, plugged and sterile Order Number Total Volume/ Graduation ml without tip 25/bag, 1,000/case ml with tip 25/bag, 1,000/case Aspiration pipette, polystyrene For the aspiration of fluids using a vacuum pump Certified non-pyrogenic Order Number Total Volume/ Graduation ml / without graduation unplugged / w/o print / ind. wrapped, sterile 1,000/case Gamma-irradiated inoculation loops, needles and spreaders from polystyrene Colour μl loop blue 10/peel pack, 1,000/case μl loop blue 48/peel pack, 1,920/case μl loop white 10/peel pack, 1,000/case μl loop white 48/peel pack, 1,920/case needle orange 10/peel pack, 1,000/case needle orange 50/peel pack, 2,000/case inoculation spreader blue 1/bag, /case inoculation spreader blue 4/peel pack, /case Serological Pipettes Serological pipettes 1 ml, 2 ml, 5 ml, 10 ml, 25 ml, 50 ml Order Number Total Volume/ Graduation Sarstedt s serological pipettes are made from a clear polystyrene featuring reverse graduation for a variable mode of handling. An additional negative scale enables a larger pipetting volume. The optimised mouthpiece ensures a universal, drip-free fit in most common pipettors. The pipettes come with a printed international colour code for easy identification of Colour Code * 1 ml 1/100 ml plugged, ind. wrapped, sterile yellow 1, ml 1/100 ml plugged, sterile, 25 pcs. yellow 1, * 2 ml 1/100 ml plugged, ind. wrapped, sterile green 1, ml 1/100 ml plugged, sterile, 25 pcs. green 1, * 5 ml 1/10 ml plugged, ind. wrapped, sterile blue ml 1/10 ml plugged, sterile, 25 pcs. blue * 10 ml 1/10 ml plugged, ind. wrapped, sterile orange ml 1/10 ml plugged, sterile, 25 pcs. orange * 25 ml 2/10 ml plugged, ind. wrapped, sterile red * 25 ml 2/10 ml plugged, sterile, 20 pcs. red * 50 ml 1/2 ml plugged, ind. wrapped, sterile violet 100 *non-pyrogenic individual volumes. Non-pyrogenic options are individually wrapped and sterile. 5 and 10 ml Pipettes without tip, e.g. suitable for homogenised media Order Number Total Volume/Graduation ml 1/10 ml plugged, sterile,10/bag /case ml 1/10 ml plugged, sterile,10/bag /case Swabs Sarstedt s swabs are ideal for the collection and transport of bacteriological, serological and cytological specimens. They are intended for use on intact skin and in natural body openings. The swabs are also suitable for health care Neutral swabs, sterile ø/tube length monitoring in the food industry as well as for sampling from a variety of surfaces. In addition to short and long swab options, the range includes plastic or aluminium stems as well as versions with and without a transport medium. For extended Stem / length Swab transport distances or the transport of fastidious micro organisms we suggest using swabs with a transport medium. The addition of carbon to the medium of certain options serves to neutralise bacterial toxins and other inhibiting substances /101 plastic/85 cotton /case /130 wood/120 cotton /case /155 plastic/135 viscose 1,000/case /155 aluminium/145 viscose 1,000/case Swabs with Amies transport medium, sterile Transport tube and separate swab, individually wrapped in convenient peel pack, sterile Transport medium in accordance with DIN 58942, Part 4 TP-AL Suited for aerobic and anaerobic bacteria Swab with Amies transport medium plus charcoal, sterile ø/tube length ø/tube length Stem / length Stem / length in mm Swab Swab /155 plastic/145 viscose /case /155 aluminium/140 viscose /case /155 plastic/145 viscose /case /155 aluminium/140 viscose /case 6 7

5 Sicherheit Faeces... Urine Sicherheit Transport... System Faeces Diagnostics Urine Diagnostics Faeces tubes from Sarstedt enable a clean and easy collection of faeces samples. Various sizes and sampling scoops are available, including an option to collect a defined faeces volume of 1 ml, approx. 1 g. The range includes sterile, labelled or opaque options. On request, faeces tubes with customised label are available at a minimum ordering quantity of 15,000 pcs. Screw cap faeces tubes ø/tube length Tube /107 PP sterile /107 PP non-sterile /101 PP sterile /101 PP non-sterile /76 PP sterile /76 PP non-sterile /76 PP sterile, labelled Sarstedt s range of urine collection products includes two systems: The Urine Monovette works using the classical aspiration principle where urine is collected by placing the suction tip into a urine sample and withdrawing the piston (Fig. a). The V-Monovette Urine is a vacuum system for enclosed urine transfer. This system provides enhanced hygiene and convenient handling for patients and users. It is not necessary to open a urine cup or container to fill the tube so that contamination is practically excluded (Fig. b). Both systems are available with a boric acid preparation to stabilise the microorganisms contained in the urine for up to 48 hours at room temperature. (Fig. b) /76 PP /76 PP, white non-sterile, labelled non-sterile, labelled Urine culture Volume in ml (Fig. a) ø/length Faeces tube with flat base and push cap Urine-Monovette with stabiliser, ind. wrapped, sterile 10 15/102 ø/tube length Tube V-Monovette Urine with stabiliser, round base 4 13/ /75 PS sterile V-Monovette Urine with stabiliser, round base 10 15/ /75 PS non-sterile Screw cap tube for the collection of a defined faeces sample Accessories This faeces tube enables a clean and simple separation of a defined faeces sample of 1 ml, approx. 1g. The spoon has a capacity of 1 ml and any excess is removed with an enclosed spatula. The spoon is fixed to the cap and sits halfway down the tube when capped. This construction enables centrifugation and separation of a supernatant without disturbance upon opening Cup with assembled screw cap, sterile, with safety label Volume in ml ø/length /73 5/bag 200/case ø/tube length Tube /101 PP Faeces tube incl. spatula 1,000 Please refer to our brochures Urine Analysis and V-Monovette Urine with additional products for urine analysis Cup with integral transfer device, assembled lid, sterile, with safety label /73 5/bag 200/case Transport System 25 ml and 70 ml Faeces containers The 25 and 70 ml tubes are made from a durable white polypropylene. The brown screw cap includes an integral sampling scoop. While containers are primarily used for the collection of faeces samples for pathology, they are also very convenient for the collection of grain and soil samples. ø/length Container Volume in ml /54 PP /55 PP 70 White, with brown cap and label White, with brown cap and label Our range includes a complete packaging system of mailing containers/ tubes and boxes for the transport of urine and faeces samples. Tested by the Federal Institute for Material Research and Testing (BAM) in Germany, the system has been approved to comply with the requirements of P650 Instructions for Class UN 3373 of the ADR, RID, ICAO and IATA. These instructions require a transport packaging consisting of 3 components: the primary receptacle, the secondary packaging and a protective outer packaging. As established by ADR, many Sarstedt primary tubes receptacles (faeces tubes, Urine Monovette ) are designed to withstand, without leakage, an internal pressure difference of at least 95 kpa (0.95 bar) for the safe and optimal transportation of diagnostic specimens in accordance with relevant requirements. Please refer to our brochure No Transport and Mailing Systems for detailed information. 8 9

6 Sicherheit Disposal Bags... Sicherheit MegaBlock... Rack Series 20 Disposal Bags MegaBlock Sarstedt s disposal bags are ideal for the containment and disposal of contaminated laboratory and hospital consumables. A robust 50 μm thick polypropylene bag with a durable, wide base seam ensures a good resistance to tear and puncturing. However, in view of the inherent injury risk, sharp or pointed objects must never be placed into the bags. Sarstedt s disposal bags are suited for hot steam sterilisation in the autoclave from 121 C (1) to approx. 134 C (2). For a complete steam sterilisation, the disposal bags must always be open during autoclaving. The range includes a variety of sizes and colours as well as options with Bio Hazard print. Durable (50 μm) for improved safety Reduction of waste volume Suitable for autoclaving at 121 C (1) to approximately 134 C (2) Table rack for disposal bags Steel rack coated with epoxy resin 1 rack including dispenser box with bags ( ) The 96 well MegaBlock meets all important requirements for processing sample volumes from 1.2 to 2.2 ml in automated systems or for storing large quantities of backup samples Tape/mat covers are included in the range. Products are manufactured under stringent clean room conditions and are protected against contamination. Free from human DNA, DNase/ RNase, endotoxins MegaBlock 1.2/2.2 ml, PP Each well 100% leak-proof tested Ideal for backup storage of pharmaceutical substances Ideal for DNA isolation Solvent-resistant to DMSO Suited for enzyme assays and tissue culture applications Round bottom wells with alphanumeric coordination system Ideal for long-term sample storage Autoclavable* MegaBlock 1.2 ml, transparent, PS Each well 100% leak-proof tested Ideal for long-term storage of blood samples Pierceable mat cover Transparent for superior visual control Round bottom wells with alphanumeric coordination system MegaBlock Volume/Material round wells 1.2 ml PS transparent round wells 1.2 ml PP square wells 2.2 ml PP 32 Mats and sealing tapes for MegaBlock Mat cover for 1.2 ml MegaBlock, pierceable, compatible with and Mat cover for 2.2 ml MegaBlock, compatible with pcs./inner box 10 pcs./bag pcs./inner box 10 pcs./bag Aluminium tape, opaque 100 tapes/inner box Acetate tape, transparent 100 tapes/inner box Rack System for Faeces and Urine Samples Disposal bags Opening dimension x length (mm) Bag colour Bag print Print neutral yellow yes no red blue * 200 x 300 (1) 100/1, x (2) 50/ x (1) 50/ Four different racks to accommodate a range of diameters from 17.2 to 26 mm are available for spacesaving and systematic backup storage of faeces and urine samples. The high-grade, break-resistant is durable and can be autoclaved at 121 C*. Racks can be easily Rack Series 20 disassembled for cleaning. A version with side pocket for documents is also available. A cover compatible with this rack protects open tubes against contamination. * Please refer to our general catalogue for information on additional rack series x (2) 50/ x 780 (2) 50/ x 780 (1) 50/ x 780 (2) 50/ x 780 (2) 50/ x 780 (1) 50/ x 780 (2) 50/ x (2) 50/ x (2) 50/150 *Disposal bag for table rack in convenient dispenser box Aperture diameter top centre bottom Outer dimensions WxHxL Application (examples) x 60 x 327 Tubes up to 25 mm Ø x 60 x 327 Tubes up to 21 mm Ø x 55 x 257 Tubes up to 17 mm Ø, all S-Monovettes x 55 x Cover in brown, translucent 62 x 72 x 256 Rack with side-pocket for documents, tubes to 17 mm Ø, all S-Monovettes Compatible with rack ; the cover protects open tubes against contamination * Important notes on autoclaving: Products made from PP can be autoclaved up to 121 C without significantly impairing their mechanical properties. Users are to test for themselves if autoclaving may have any effect on other characteristic product features so as to influence the individual application concerned

7 You may also be interested in the following subjects... POS 720/PTS Automation in Microbiology Urine Analysis Urine Monovette Urine Monovette with stabilizer UriSet 24 Urine Containers Urine Cups V-Monovette Urine The vacuum system for enclosed urine transfer NEW! : : : Transport and Mailing Systems For transportation of diagnostic samples and blood products PCR & Molecular Biology Certified High-Quality Products for PCR, Molecular Biology and Research Cell and Tissue Culture Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling The complete product range for mailing and transport requirements Liquid Handling Safe and compliant with transportation guidelines 537a This document may contain information on products that may not be available in particular countries Technical modifications reserved : CryoPure Storage System Energizing your Cryopreservation : SARSTEDT AG & Co. P.O. Box D Nümbrecht Phone Fax info@sarstedt.com Efficient and reliable cryopreservation : Screw cap micro tubes For transport, storage and sample preparation : For over years Reliable quality and versatility : Forensic Swab in a transport tube with ventilation membrane Maximum purity for optimal safety in preanalytics The new Forensic Swabs from Sarstedt are suited for the collection of both reference samples and evidence of DNA traces at crime scenes. Apart from Forensic Swabs with polystyrene stem, our range also includes a wooden stem option. The Forensic Swab L has an extended handle for the hygienic collection of reference samples. The Forensic Swab XL with extended stem is ideal for the collection of evidence in sexual assault cases. The ventilation membrane in the tube base enables self-drying of the swab within the tube. Drying of the swab in the open, i.e. outside the tube, as in standard systems is unnecessary, thereby excluding the risk of mix-up and contamination. Stringent purity conditions in the production process and a special EtO sterilisation method developed by Sarstedt to comply with the requirements of forensic analysis ensure the absence of DNA contamination. DNA-free EtO sterilised Self-drying within the tube Identification code* for correct matching Individually wrapped, sterile, or with tamper-proof closure * Global article ID number + serial number (SGTIN)/GS1 Germany. The code is printed on the label both in plain script and in a GS1 DataMatrix format on the tube and swab for a correct match (Art. Nos and only). : NEW!