Biotechnology. Biotechnology s Laboratories. Lab Name Location Person in Charge Programs Served Courses Served. Biotechnology Department

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1 Biotechnology s oratories Biotechnology Name Location Person in Charge Programs Served Courses Served General Biology W Zahra Yassin General Microbiology M Aisha Echtibi General (Basic Course) Tissue Culture & Hybridoma Technology Molecular Genetics Molecular Biology Plant Biotechnology Microbial Processing & Biotechnology Environmental Biotechnology Immunology & Serology W Aisha Echtibi Immunology & Serology Tissue Culture & Hybridoma Technology M Mona Ibrahim Tissue Culture & Hybridoma Technology Molecular Genetics W Mona Ibrahim Molecular Biology Molecular Biology W Zahra Yassin Molecular Biology Student Research Project General Biochemistry W Aisha Echtibi General Biochemistry Student Research Project Protein Biochemistry & Engineering W Zahra Yassin Protein Biochemistry & Engineering Student Research Project Plant Biotechnology W Mona Ibrahim Plant Biotechnology Student Research Project Microbial Processing & Biotechnology W Zahra Yassin Microbial Processing & Biotechnology Student Research Project Environmental Biotechnology M Mona Ibrahim Environmental Biotechnology Student Research Project 133

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3 General Microbiology Introduction The laboratory is designed to provide practical and hands-on experience of a variety of microbiological techniques. oratory work includes microscopy, staining, aseptic and sterile transfer techniques, assessment of morphological and biochemical properties of microorganisms and microbial identification. Experiments Safety Microcopy and Simple Staining Culturing and Isolation Techniques Selective and Differential Staining of Microbes Microbial Morphology Selective and Differential Media & Demonstration of Bacterial Culture Characteristics Control of Microorganisms by Physical & Chemical Factors Bacterial Growth Curve Microbial Control Biochemical Testing Identification of Unknown 135

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5 Tissue Culture and Hybridoma Technology Introduction The course is designed to study the basic principles of animal tissue culture. The course will also introduce the student to basic hybridoma technology concepts and monoclonal antibodies production techniques. Experiments Safety & Aseptic Techniques Introduction to Cell Culture: Media & Cell Types Subculturing, Cell Quantification & Viability Studies Cell Differentiation Detection & Control: Mycoplasma, Bacteria & Fungus Cryopreservation Isolation of Primary Cells Culturing Primary Cells Growing Hybridomas Isolation of Monoclonal Antibody 137

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7 Moelcular Biology LAB Introduction This course is designed to enhance the preparation of students for careers in research, biotechnology and science education. The laboratory experience will introduce students to the field of molecular biology where they will learn all the basic methodologies and instrumentation commonly used in biotechnology and molecular biology laboratories. Students will learn bacterial transformation, plasmid DNA purification, restriction digestion and ligation along with gel electrophoresis, polymerase chain reaction (PCR), cloning DNA fragments, and selection of recombinants. This course will give students who plan career in research or biotechnology practical experience that mimics the realities of the laboratory setting. Experiments DNA isolation, Concentration Calculations, Purity Restriction Enzyme Digestion and Ligation DNA Gel Electrophoresis DNA Gel Extraction DNA Basics: Bacterial Transformation Selection of Recombinants: Antibiotic Resistance and Blue White Screening Designing Primers for PCR Amplification Reverse Transcription PCR 139

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9 Protein Biochemistry & Engineering LAB Introduction This course covers basic biochemical principles of proteins along with a comprehensive survey of products currently available or under development by the protein biotechnology industry. Experiments Protein Purification Salting Out Dialysis Gel Filtration Expression and Characterization of a Recombinant Enzyme in E.Coli. SDS Gel Electrophoresis, Molecular Weight Measurements; Protein Gel Staining (including the whole cell homogenates); Gel Drying Western Blotting Concepts; SDS PAGE and Transfer Western Blot Analysis 141

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11 Microbial Processing & Biotechnology LAB Introduction The course focuses on the vast array of applications of microorganisms. It is aimed to highlight the potential of wild or genetically modified microorganisms as cell factories for the production of goods and services. It introduces the student to the new era of biotechnology & familiarizes the student with the several areas of industrial application of microorganisms: medical and pharmaceutical, protein production (enzymes, yeast, and single cell protein), production of biochemicals (primary/secondary metabolites), food and beverage technology, agriculture and environmental technologies and alternative energies (bioenergy). Experiments Isolation of Streptomycetes from Soil Samples and Testing their Potential to Produce Antibiotics Effect of Different Sweeteners on the Fermentation of Yeast Pickle Fermentation Using Chymosin to Make Cheese Fermentation Growth Curve of Saccharomyces Cerevisiae Field Visits Al-Rawabi Dairy Farm, Dubai Central Food Control and Consultancy oratory, Sharjah Municiplaity Julphar Pharmaceutical Industries, RAK 143

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