UltraBasic Series Meter
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1 UltraBasic eries Meter Operat Manual UP-5 UP-10 UP-25 /Temp /mv/temp /mv//temp UP-10 /mv Meter Rev. A
2 UP-10 /mv Meter UltraBasic Meter Quick Reference 1. Install a 9 volt battery in the battery compartment or connect a AC adapter to the power connect (optal). 2. Connect an electrode to the Input and ATC connectors (optal). 3. Press until the display indicates the appropriate measurement (, mv/relative mv or ). 4. tandardize the meter using up to three standards by immersing the electrode in a buffer/standard, stirring, then pressing to each standard. UP-10 /mv Meter The display shows the current reading in, mv, relative mv or units. 6. Press to review electrode calibrat and to clear or select buffers/standards.
3 Table Of Contents Quick Reference Inside Front Cover Getting tarted Front panel controls Digital display Rear panel connectors Battery Installat Installing and Maintaining Electrodes Installing electrodes toring electrodes tandardizing for Measurement Entering buffers Using etup Clearing Buffers Reviewing electrode standardizat electing buffer sets tandardizing for Millivolt Measurement (Relative Millivolts) Entering a millivolt offset Using etup in mv Mode Clearing a millivolt offset tandardizing to Ion Measurement Entering standards Using etup in Ion Mode etting the values of the standard Understanding Theory Defining Understanding Temperature Compensat U.. tandard Buffer Typical electrode response and temperature Measuring Measuring Ion Concentrat Troubleshooting Electrode testing Meter Operat testing Meter pecificats Accessories Technical Assistance Back Cover 1
4 Getting tarted Front Panel Controls UP-10 /mv Meter - press to clear standards, review electrode calibrat or select new auto recognized buffers and standard sets. - press to toggle between, mv or press to select opts. - press to autorecognize each buffer or standard. Measuring Icon Digital Display power - press to turn unit on or off Low Battery Indicat tandardizing Icon tability Icon Electrode Check Icons Buffer Icons al TD %DO m mg/l /cm K µ cm rel mv M OK et Error Clear Auto Man 1 %/ Note: Not all icons on display will be used. Temperature Mode Result Prompts tandard Icons 2
5 /mv Meter Rear Panel Connectors UP-10 BNC or Ion Electrode Connector ATC (Automatic Temperature Compensat) Probe Connector AC Power Connector AC Cover Note: AC cover must be in place for IP-67 protect Battery Installat 9V Battery Battery Compartment Cover 3
6 UP-10 /mv Meter Installing and Maintaining Electrodes This meter allows you to use: Combinat electrode with BNC connector 3-in-1 combinat electrode with BNC connector and 2.5 mm phone jack for ATC eparate 2.5 mm phone jack for ATC Ion selective or ORP electrode with BNC connector. 1. Remove the protective end cover from electrode. 2. Before using your electrode, or whenever the electrode is dry, soak over-night in an Electrode Filling olut, KCI solut or Electrode torage olut. 3. Remove the shorting cap on the BNC connector. Install the electrode by plugging it into the input connect (push on and twist to lock). An accessory ATC probe can be installed in the ATC port for the most accurate measurements. e 4
7 4. Rinse and blot-dry electrodes between each measurement (do not wipe). Rinse electrodes with distilled water or deized water, or part of the next solut to be measured. 5. tore electrodes in KCI solut or Electrode torage olut. Always leave the filling hole open when in use and closed when in storage. Refill with Filling olut when the internal solut level gets 1 below fill hole. Note: The electrode that comes with the UltraBasic kit is gel-filled. It has no fill solut or filling hole. 5
8 tandardizing for Measurement Because electrodes vary in their response, you must your meter and electrode to compensate for electrode variat. The more frequently you, the more accurate your measurements. tandardize daily, or more often, for accurate results. NOTE: To escape standardizing press tandardize again 1. Immerse electrode in a buffer solut. tir gently. Allow the electrode to reach a stable value. 2. Press and release the button until your digital display indicates Clear existing buffers when doing a new 2 or 3 point standardizat. Use the and buttons to clear existing buffers. 4. Press. The meter flashes the current buffer set and recognizes the flashing buffer. When the signal is stable, or when you press, the buffer is ed. 4 7 Clear
9 5. The meter displays the percent slope of the electrode as 100.0% on the first buffer. On ing a second or third buffer, the meter performs a diagnostic check on the electrode and displays the slope. 6. To a second buffer, place the electrode in the second buffer solut, stir, allow time for the electrode to stabilize, and press again. The meter recognizes the buffer. OK Next, the meter performs a diagnostic test of the electrode. The display indicates electrode condit. The meter displays the % slope of the electrode. 8. lope Error indicates that your electrode is not working properly. The electrode response must be between 90 and 105% slope. Measurements causing lope Error are not accepted, used or stored by the meter. Press to continue. 4 7 OK 4 7 Error 7
10 9. To a third standard, place the electrode in the third buffer solut, stir, allow to stabilize, and press. The results will be the same as in steps 7 and After ing each buffer, the tandardizing icon goes off and the Measuring or table icon appears on the display to indicate that the meter returns to Measuring operat OK tandardize your meter and electrode using at least two buffers with values bracketing the expected of your samples. tirring with a magnetic stir bar and stirrer provides faster electrode response. NOTE: If an ATC probe is used, the meter continually adjusts for temperature. Therefore, buffers may vary slightly from the nominal values because of temperature. Default temperature is 25 oc NOTE: The temperature is only displayed when an ATC probe is attached. 8
11 Using etup in Mode The button lets you clear all the buffers/standards that you have ed, review calibrat informat, or select the buffer/standard set that you want. NOTE: Continuing to press etup will return you to measurement. 1. Press and the meter displays a flashing Clear icon. Use this step only when you wish to clear all buffers you have ed. To clear all existing buffers, press. The meter clears all buffers and returns to Measuring screen. 2. Press again to show electrode performance. If the meter has accepted two buffers, it will display Good Electrode, display the slope between the first and second buffers and display the two buffer icons. 4 7 Clear 4 7 OK 3. Pressing again shows the electrode slope between the second and third buffers (if three buffers have been ed) and shows the second and third buffer icons. OK Note: These screens will only be displayed if buffers are stored
12 4. Press etup again to display a flashing et Buffers icon and to display the first buffer set (U.. buffer set) icons. 5. Press Enter to select the set of buffers shown on the display or Press etup again to view the next set of buffers. Continue pressing etup to view the third buffer set (NIT buffer set). et et
13 tandardizing for Millivolt Measurement (Relative Millivolts) NOTE: UP-5 does not include mv. You use millivolt measurement for electrode diagnostics, titrat or measuring redox potential (also called ORP, oxidat reduct potential). ORP measurements indicate the oxidizing or reducing capability of a solut. You can use ORP values to monitor or control soluts requiring a set amount of oxidants or reductants. 1. Immerse electrode in a standard solut. 2. Press the button until your digital display indicates mv. mv 3. Press to a mv standard and read relative mv. 4. When the signal becomes stable, or when you press, the current absolute mv value becomes zero relative millivolts. mv rel mv 11
14 Using etup in mv Mode 1. To clear a mv offset and return to absolute millivolt, press. The meter displays a flashing Clear icon, and shows the current relative millivolt offset. 2. To clear the mv standard, press. You then return to absolute mv. rel mv mv Clear 12
15 tandardizing for Ion Measurement Because electrodes vary in their response, you must your meter and electrode to compensate for electrode variat. The more frequently you, the more accurate your measurements. tandardize daily, or more often, for accurate results. Note: You must first your standard values in. 1. Immerse electrode in standard solut. tir gently. Allow the electrode to reach a stable value. Ion elective Electrodes may take up to 10 minutes to reach a stable reading. 2. Press and release the button until your digital display indicates. Note: No measurement will display on screen if less than two standards are stored. 3. Clear existing buffers when doing a new 2 or 3 point standardizat. Use the and buttons to clear existing standards. 4. Press. The meter flashes 1. After 2 seconds 1 the will appear. When the signal is stable, or when you press, the standard is ed. Clear Note: If desiring to only 2 and 3, press the key when 1 flashes. The meter will at 2.
16 5. The meter returns to measurement and is displayed 1 indicating the first standard is stored. Note: No measurement will display on screen if less than two standards are stored. 6. To a second standard, place the electrode in the second standard solut, stir, allow time for the electrode to stabilize, and press again The meter will flash 2 and 2 after two seconds the will appear. When the signal is stable, the meter recognizes the standard and 2 are both displayed along with the change in mv/decade. Typical mv change for a monovalent IE is 59 mv/decade and 27 mv/decade for a divalent IE. Refer to the IE instructs for acceptable mv change
17 9. lope Error indicates that the change in mv between the standards displayed is too small and accurate measurements cannot be taken. Press to continue. 10. To a third standard, place the electrode in the third standard solut, stir, allow to stabilize, and press. The results will be the same as in steps 7 and 8. Error After ing each standard, the tandardizing icon goes off and the Measuring or table icon appears on the display to indicate that the meter returns to Measuring operat. 12. tandardize your meter and electrode using at least two standards with concentrats bracketing the expected range of your samples. tirring with a magnetic stir bar and stirrer provides faster electrode response
18 Using etup in Ion Mode The button lets you clear all the standards that you have ed, review calibrat informat, or the standard values that you want. NOTE: You can escape at any time by pressing. 1. Press and the meter displays a flashing Clear icon. Use this step only when you wish to clear all standards you have ed. To clear all existing standards, press. The meter clears all standards and returns to Measuring screen. 2. Press again to show electrode performance. If the meter has accepted two standards, it will display OK, and display the mv /decade change between the first and second standards. Clear Press again to show mv/decade change from 2 and 3 (the second and third standards). 2 3 Note: These screens will only be displayed if standards are stored. 16
19 4. Press again and et and 1 will begin to flash. Press to set the value of the first standard. 5. The current value set for the first standard will be displayed (00000 is displayed if no value is set). The first digit is flashing and is ready to be set. et et 1 1 To increase the number press. To decrease the number press. To move to the next digit press. 17
20 6. Press until the correct digit is displayed. 7. Press the key to move to the second digit. 8. Repeat steps for all digits. After the last digit, press until the decimal place is in the proper locat. Note: To clear standard value setting press until clear is displayed and press. et et Press to accept the value. If the value needs to be changed, press key again. 10. Press and repeat steps 4-9 for 2 and 3. et et
21 Understanding Theory Defining The measurement of plays an important role in identifying and controlling acidity and alkalinity levels for industry and research. is a measure of the acidity or alkalinity of a solut and can be represented by this equat: = -log [H + ] with [H + ] representing the concentrat of hydrogen s in the solut. is sometimes referred to as the power of the hydrogen in a solut. By using a meter, you can determine exact levels of soluts. For example, rather than say that lemon juice is quite acidic, you can say that lemon juice has a of 2.4. An exact value can be used to control or measure acidity levels for manufacturing processes or for basic research. values generally range from 0 to 14, with a value of 7 being the neutral point, or the value of pure water. values greater than 7 represent increasing alkalinity, whereas values below 7 represent increasing acidity (Figure 1). Neutral trong Acid Lemon Juice Tomato Juice Coffee Pure water Baking oda Ammonia trong Base more acidic more basic Figure 1. cale showing the relative acidity or basicity of some common substances. 19
22 Understanding Temperature Compensat NOTE: Temperature compensat only takes effect when an ATC probe is attached. There are two ways that temperature compensat affects measurement: 1. Buffer values change with temperature. Each buffer changes depending on the temperature of the solut. These values are typically found on the label of the buffer. The tables below are representative of most buffers. When standardizat is performed in, the value is adjusted to the current value for the current temperature. For example, if your 7 buffer is at 20 the meter will your buffer at 7.02 instead of 7.00 which is the value at The electrode efficiency changes with temperature. The standard mv change per unit is approximately at 25. However, as shown in the below graph, this mv per unit value changes as temperature changes. U.. tandard Buffer The meter compensates for this change by changing the reading as temperature changes. electrode potential (mv) (74mV/ unit) 50 (64mV/ unit) 0 (54mV/ unit) isopotential point Typical electrode response as a funct of temperature 20
23 Measuring To measure with a convental glass electrode, the meter uses a -sensing glass bulb that is sensitive to hydrogen s. The potential developed at the glass membrane is directly related to the of the solut. The glass electrode is paired with a reference electrode which completes the electrical measuring circuit and provides a stable reference point. These two electrodes are joined to create a combinat electrode. The combinat glass electrode is connected to the meter which reads the voltage, converts it to units, and displays the result. ATC Temperature ensor ensor Porous Junct Reference Electrode 21
24 Measuring Ion Concentrat The UP-25 not only has the capability of measuring and mv but also measures concentrat. In you can measure the concentrat of specific s using an selective electrode (IE). The concept is the same as in where the activity of hydrogen s is measured with a electrode. In the mv values of buffers are plotted against the buffer value to generate a standardizat curve. The same can be done for other s since selective electrodes follow a Nernstian electrode slope. By plotting log concentrat versus mv value of the standards a standardizat curve can be generated. This standardizat data is then used to take the mv reading, of unknown samples, and determine concentrat of those samples. A typical slope for a monovalent IE is 59 mv/decade and 27 mv/decade for a divalent IE. However at lower concentrats (typically less than 10 ppm) the response of the electrode starts to deviate from the Nernstian curve. This is due primarily to the low activity of the as well as the solubility of the membrane. It is very important to at several points if measurements are typically in this range. Choosing standardizat points You must at a minimum of two points for the UP-25 meter to begin taking measurements. You should choose standards that bracket the concentrat range of your samples. We would recommend using standards that are ten-fold apart. o if the expected concentrat of your samples were between 15 and 50 ppm, we would recommend that you at 10 ppm and 100 ppm. You can up to three points. o if your sample range is from 15 ppm to 1500 ppm you should at 10 ppm, 100 ppm and 1000 ppm. This meter can only take measurements from 0 to ppm. All standards values need to be below Preparing your standards Typically standard soluts are purchased at a higher concentrat than is needed for standardizat. Then diluts are made, using volumetric pipettes and flasks, to the required concentrats. Generally ic strength adjuster (IA) is added to the standards and samples to speed up electrode response time, eliminate background noise, and adjust the to the desired range. Typically 1 ml of IA should be added for every 50 ml of standard and sample. 22
25 tandardizing After setting the value of the standards (see Using etup in Ion Mode) it will be necessary to the electrode. Place the electrode in the lowest concentrat standard to be d. Typically the recommended storage solut for selective electrodes is the lowest concentrat standard regularly measured so it should not take too much time for the electrode to stabilize. You can press "Mode" until mv is displayed to view the stability of the result. Follow the steps in the "tandardizing for Ion Measurement" sect to complete standardizat taking care to wait for a stable result before pressing the tandardize key. Taking measurements After standardizat is complete, you are ready to take measurements. Add IA to your sample(s). As soon as you put the electrode in the sample, measurements begin. The reading is stable when both the "" icon appears and when the result is no longer changing. The meter displays three significant figures. Errors The most common errors in measurement are: Dilut errors Entering standards before the reading is stable Impurities in water and/or dirty glassware Improper care/storage of IE 23
26 Troubleshooting 1. If the signal from the electrode is out of range, the display will show This may happen when the electrode is not in a solut. 2. The meter will display Electrode Error when it detects an error in electrode response. During standardizat, the message indicates that the electrode is less than 90% or more than 105% of the correct response in. The Electrode Error message can indicate either a bad electrode or bad buffer(s). 4 7 Error 3. If the meter detects an error in the temperature probe, the display shows If you do not use a temperature probe, the meter uses the default temperature of To test the electrode, place it in a good 7 buffer. Press to use the mv (UP-10 and UP-25 only), and note the millivolt reading. Make sure the meter is in mv and not relative mv. Repeat for either a 4 or 10 buffer. The electrode signal must be within the limits shown below (when temperature is near 25). Electrode Test 7 0 ± 30 mv to 186 mv more than to 186 mv less than 7 24
27 UP-10 /mv Meter 5. To test the meter for correct operat, install the BNC (input) shorting cap. Press to select the mv (UP-10 and UP-25 only), and note the mv reading. Make sure the meter is in mv and not relative mv.if the meter reads mv, it is measuring correctly. Note that a long term drift of 0.1mV/month since last calibrat is specified. 25
28 Accessories You can order the following accessories for your meter: Replacement UltraBasic electrode Plastic-body /ATC Electrode High-performance glass-body /ATC Electrode ATC Temperature Probe Free-standing Electrode Arm with Base Transformer, 115 V NA Other electrodes, such as selective electrodes and redox electrodes are also available. Call your sales representative. Meter pecificats Range to Resolut 0.01 Accuracy 0.01 tandardizat 1, 2 or 3 buffers Auto buffer recognit 16 buffers 2, 4, 7, 10, 12 1, 3, 6, 8, 10, , 4.01, 6.86, 9.18, mv Range to mv (UP-10 & Resolut 0.1 mv UP-25 only) Accuracy 0.2 mv or 0.05%, whichever is greater Ion Range to (UP-25 only) Resolut 3 sig figs Accuracy 0.34% tandardizat 2 or 3 standards Temperature Range -5.0 to Resolut 0.1 Accuracy 0.4 Auto Temperature Compensat with ATC probe Automatic electrode slope correct for % 26
29 Warranty Instructs 1. Please return the prepaid, pre-addressed Purchase Registrat Card to Denver Instrument Company promptly upon your purchase of the Denver Instrument product. The return of the card is not a condit precedent to warranty coverage. 2. If you have any quests about a Denver Instrument product, please contact the nearest Denver Instrument office as listed below. 3. If it becomes necessary to return your Denver Instrument product for service, you must obtain a Return Authorizat Number. Please pack the product securely in its original approved packing carton or an other suitable container. Include your Return Authorizat Number on the shipping label. hipping charges must be fully prepaid. Return to authorized distributor or : North and outh America: U.K. and Ireland: Europe, Asia and Australia: Denver Instrument Company ervice C 6542 Fig treet Arvada, Colorado Tel: Tel: Fax: Denver Instrument Company Denver House, overeign Way Trafalgar Business Park Downham Market Norfolk PE38 9W England Tel: Fax: Denver Instrument GmbH Robert-Bosch-Briete Gottingen Germany Tel: Fax: Disclaimers You have purchased a quality precis meter that requires handling with care. Read entire contents of this Operat Manual prior to operating your new Denver Instrument meter. Important Notes: IP-67 rating is valid only when AC cover is completely installed and securely in place. The operator shall be responsible for any modificats to Denver Instrument Company equipment and for any connects of cables or equipment not supplied by Denver Instrument and must check and if necessary, correct these modificats and connects. Changes or modificats not expressly approved by the manufacturer could void the product s warranty.
30 North and outh America: U.K. and Ireland: Europe, Asia and Australia: Denver Instrument Company 1855 Blake treet, uite 201 Denver, Colorado Tel: Tel: Fax: Denver Instrument Company Denver House, overeign Way Trafalgar Business Park Downham Market Norfolk PE38 9W England Tel: Fax: Denver Instrument GmbH Robert-Bosch-Briete Gottingen Germany Tel: Fax:
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