Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer

Size: px
Start display at page:

Download "Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer"

Transcription

1 Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer Matt Berube, Joe Dibussolo, Catherine Lafontaine, Yang Shi, and Francois Espourteille Thermo Fisher Scientific, Franklin, MA

2 Overview Purpose: A high throughput method for the quantitation of vitamin D using online sample preparation and high resolution, accurate mass (HRAM) quantitation with an Exactive Plus orbitrap mass spectrometer for clinical research use. Methods: Solvent precipitated serum is injected onto an extraction column under turbulent flow conditions. A 2 microliter loop containing organic solvent is used to transfer the Vitamin D to a solid core HPLC column for separation and analysis using an Exactive Plus Orbitrap MS. Results: The method provided reliable quantitation of 25-hydroxyvitamin D 2 and 25- hydroxyvitamin D 3 levels in serum with a lower limit of quantitation of 2 ng/ml. Introduction Blood levels of 25-hydroxyvitamin D 2 and 25-hydroxyvitamin D 3 are commonly tested by clinical researchers. In the last decade, liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) has become a popular technique for vitamin D testing. Due to their higher resolving power over triple stage quadrupole mass spectrometers, high-resolution benchtop orbitrap mass spectrometers are better able to resolve analytes from sample matrix. In addition, the ease of initial method set up and daily use provide an advantage over triple quadrupole mass spectrometers for clinical research. A method has been created that allows the precipitated serum to be injected onto an HPLC system with minimal sample preparation and analyzed by an Exactive Plus benchtop Orbitrap mass spectrometer. Total method time is 7.75 minutes on a Transcend TLX-1 system. Throughput can be increased to a sample every 3.7 minutes by using a Transcend TLX-2 multiplexed UHPLC system or 1.9 minutes for a Transcend TLX-4 system. FIGURE 1. Liquid Chromatography M FIGURE 2. Plumbing Diagram for onli Methods Standards Standard solutions of 25-hydroxyvitamin D 2, 25-hydroxyvitamin D 3, and deuterated 25- hydroxyvitamin D 3 internal standard were obtained from Cerilliant, Inc. Six calibrators at 2, 5 1, 25, 5 and1ng/ml and three QCs at 5ng/mL, 4 ng/ml and 8 ng/ml were prepared by fortifying bovine serum albumin diluent with 2 ng/ml 25- hydryoxyvitamin D 2 and D 3 standard mix. Precipitating reagent was prepared by adding deuterated D 6-25-hydroxyvitamin D 3 to acetonitrile for a final concentration of 75 ng/ml. In addition, pooled patient serum samples were crashed 2 to 1 with acetonitrile and spiked with analytes for a final concentration of 2 ng/ml for 25-hydroxyvitamin D 2 and 25-hydroxyvitamin D 3, and 5ng/mL of D 6 25-hydroxyvitamin D 3 internal standard. Sample Preparation 1. Aliquot 1 µl of calibrators and controls into centrifuge tubes. 2. Add 2 µl of precipitating reagent containing internal standard to each tube 3. Vortex each tube for 3 seconds. 3. Centrifuge tubes at 5, RCF for 1 minutes. 4. Aliquot supernatants into autosampler vials for analysis. The supernatant should contain internal standard at a concentration of 5 ng/ml. Liquid Chromatography Transcend TLX-2 system in focus mode configuration (Figure 2). Extraction column: Thermo Scientific TurboFlow TM XL C-18P.5x 5 mm Separation column: Thermo Scientific Accucore TM C18 3 x 5 mm 2.6uM Injection Volume: 5µL Mobile Phases: Loading A, Eluting A: Water.1% Formic Acid Loading B, Eluting B: Acetonitrile.1% Formic Acid Loading C: 4% Acetonitrile, 4% Isopropanol, 2% Acetone Total method time: 7.75 minutes Cycle time when multiplexed: 3.7 minutes Mass Spectrometry The Thermo Scientific Exactive Plus be with an APCI source in positive ionizati 35 to 425 m/z, using a lock mass of 3 was added in order to maintain mass a Mass Spectrometer Parameters: Scan Mode: Full Scan Range: m/z Fragmentation: None Resolution: 7, Polarity: Positive Microscans: 1 AGC Target: 3e6 Maximum Inject Time: 2 Data Analysis Calibration curves and QCs were run i addition, 8 pooled serum sample rep hydroxyvitamin D 2 and 25-hydroxyvitam D 3 internal standard were injected in or Scientific Xcalibur software was use 2 Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer

3 D using online uantitation with an. column under solvent is used to and analysis using itamin D 2 and 25- of 2 ng/ml. e commonly tested coupled with triple r technique for age quadrupole trometers are better of initial method set s spectrometers for pitated serum to be d analyzed by an d time is 7.75 sed to a sample system or 1.9 FIGURE 1. Liquid Chromatography Method FIGURE 2. Plumbing Diagram for online TurboFlow Extraction Results Summary The lower limit of quantitation (LLOQ) w in BSA as indicated in Figure 3. Limits o triplicate injections of the standard solut and the coefficient of variation (CV) valu ng/ml for both 25-hydroxyvitamin D 2 an improvement over the LLOQ of 1 ng/m Orbitrap MS. The correlation coefficien regression analysis of the standard curv (Figure 6). A relative standard deviation serum fortified with analytes at 2 ng/m for a total internal standard concentratio injections were less than 1 percent for performed using a neat standard of 2n hydroxyvitamin D 3 with 5 ng/ml D ten times on the TurboFlow column and analytical column only, and area counts 97 percent and 99 percent for 25-hydro respectively. Consistent results were ac of 25-Hydroxyvitamin D 2 and 25-hydrox hydroxyvitamin D 3 up to about 6 injec FIGURE 3. Chromatograms at LLOQ of RT: SM: 5G 1 RT: , and deuterated 25- Inc. ng/ml, 4 ng/ml and t with 2 ng/ml 25- s prepared by adding entration of 75 to 1 with acetonitrile 5-hydroxyvitamin D 2 3 internal standard. RT: RT: SM: 5G RT: RT: SM: 5G 1 bes. dard to each tube e supernatant L. ). 5 mm 2.6uM Mass Spectrometry The Thermo Scientific Exactive Plus benchtop orbitrap mass spectrometer was used with an APCI source in positive ionization mode. Full scan data was collected from 35 to 425 m/z, using a lock mass of (Di iso-octyl Phthalate). Lock mass was added in order to maintain mass accuracy during the batch runs. Mass Spectrometer Parameters: Scan Mode: Full Ion Source: APCI Scan Range: m/z Sheath Gas Flow Rate: 3 units Fragmentation: None Aux Gas Flow Rate: 5 units Resolution: 7, Sweep Gas Flow Rate: 1 unit Polarity: Positive Discharge Current: 3.5 µa Microscans: 1 Capillary Temperature: 25 C AGC Target: 3e6 S-Lens RF Level: 6 Maximum Inject Time: 2 Vaporizer Temp: 5 C Data Analysis Calibration curves and QCs were run in triplicate each day across four days. In addition, 8 pooled serum sample replicates containing 2 ng/ml 25- hydroxyvitamin D 2 and 25-hydroxyvitamin D 3 and 5ng/mL of D 6-25-hydroxyvitamin D 3 internal standard were injected in order to test robustness of the method. Thermo Scientific Xcalibur software was used to collect data and analyze the results FIGURE 4. Chromatograms of 4 ng/m RT: SM: 5G RT: Hyd RT: Hydroxyvita RT: d6 25-Hydroxyvita Thermo Scientific Poster Note PN6368_E 6/12S 3

4 Results Summary The lower limit of quantitation (LLOQ) was determined to be 2 ng/ml for both analytes in BSA as indicated in Figure 3. Limits of quantitation (LOQs) were estimated from the triplicate injections of the standard solutions. The signal to noise was greater than 1 and the coefficient of variation (CV) values were less than 5 percent at the LLOQ of 2 ng/ml for both 25-hydroxyvitamin D 2 and 25-hydroxyvitamin D 3. This is an improvement over the LLOQ of 1 ng/ml that was previously achieved on the Exactive Orbitrap MS. The correlation coefficients obtained using 1/X weighted linear regression analysis of the standard curves were greater than.99 for both analytes (Figure 6). A relative standard deviation (%RSD) test was performed in pooled human serum fortified with analytes at 2 ng/ml and crashed with internal standard solution for a total internal standard concentration of 5 ng/ml. The RSDs of ten replicate injections were less than 1 percent for both analytes. A recovery study was also performed using a neat standard of 2ng/mL 25-hydroxyvitamin D 2 and 25- hydroxyvitamin D 3 with 5 ng/ml D 6-25-hydroxyvitamin D 3. The standard was injected ten times on the TurboFlow column and analytical column, and ten times on the analytical column only, and area counts were compared. The relative recoveries were 97 percent and 99 percent for 25-hydroxyvitamin D 2 and 25-Hydroxyvitamin D 3, respectively. Consistent results were achieved with pooled serum containing 2 ng/ml of 25-Hydroxyvitamin D 2 and 25-hydroxyvitamin D 3 and 5 ng/ml D hydroxyvitamin D 3 up to about 6 injections as indicated in Figure 7. FIGURE 3. Chromatograms at LLOQ of 2 ng/ml with 5 ng/ml internal standard RT: SM: 5G RT: 1.67 NL: 1.1E5 25-Hydroxyvitamin D [ ] MS FIGURE 5. QC Replicate Injections. each day over four days and results c FIGURE 6. Calibration Curves Vit Vit RT: SM: 5G RT: 1.64 NL: 1.73E5 25-Hydroxyvitamin D [ ] MS.4 25-OH Y = ctrometer was used as collected from thalate). Lock mass uns RT: SM: 5G RT: 1.63 NL: 8.39E6 1 d6 25-Hydroxyvitamin D [ ] MS FIGURE 4. Chromatograms of 4 ng/ml QC injection : 3 units units : 1 unit µa 25 C s four days. In L hydroxyvitamin he method. Thermo yze the results. RT: 1.67 NL: 1.43E Hydroxyvitamin D [ ] MS RT: 1.64 NL: 2.13E Hydroxyvitamin D F: FTMS 8 + p APCI corona Full 7 l ock ms [ ] MS 6 QC RT: 1.63 NL: 7.96E6 1 d6 25-Hydroxyvitamin D [ ] MS OH Y = Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer

5 FIGURE 5. QC Replicate Injections. Three replicates of each QC level were run each day over four days and results compared. FIGURE 7. Serum injection replicat variability increased. Vitamin D 2 ml for both analytes estimated from the as greater than 1 nt at the LLOQ of 2 his is an eved on the Exactive hted linear for both analytes ed in pooled human l standard solution of ten replicate study was also 2 and 25- andard was injected times on the ive recoveries were xyvitamin D 3, containing 2 ng/ml D e 7. nternal standard NL: 1.1E Day Vitamin D Day Area [ ] MS NL: 1.73E [ ] MS FIGURE 6. Calibration Curves.4 25-OH_Vitamin_D2 Y = *X R^2 =.994 W: 1/X Variability increased during replicate injections. The system ran serum inj blanks or washing in between injectio mass spectrometer source becoming cleaning. Future experiments will se variability occurs, possibly by increas occurs during the HPLC method, as w during the sample run NL: 8.39E [ ] MS Conclusions Reliable detection of 25-hydrox by clinical researchers. Higher resolving power with Ex NL: 1.43E ng/ml Lower LLOQ with Exactive Plu Cycle time of 1.9 minutes when Method robustness of approxim changes. [ ] MS 25-OH_Vitamin_D3 Y = *X R^2 =.9989 W: 1/X Ease of method set up compar References NL: 2.13E F: FTMS + p APCI corona Full l ock ms [ ] MS QC NL: 7.96E Shah I, James R,Barker J, Pet in Vitamin D analysis: A nove isobars. Nutrition Journal 211 Acknowledgement We would like to thank Keeley Murph [ ] MS ng/ml All trademarks are the property of Thermo Fisher Sc This information is not intended to encourage use o intellectual property rights of others. For Research U Thermo Scientific Poster Note PN6368_E 6/12S 5

6 level were run FIGURE 7. Serum injection replicates. Approximately 6 injections before variability increased Area Vitamin D3 Vitamin D Injection Number W: 1/X Variability increased during replicate serum injections after approximately 6 injections. The system ran serum injections constantly during this time period with no blanks or washing in between injections. Increased variability could be due to the mass spectrometer source becoming dirty after so many serum injections with no cleaning. Future experiments will seek to extend the number of injections before variability occurs, possibly by increasing the amount of washing of the columns that occurs during the HPLC method, as well as steps to keep the MS source cleaner during the sample run. Conclusions Reliable detection of 25-hydroxyvitamin D 2 and 25-hydroxyvitamin D 3 in serum by clinical researchers. Higher resolving power with Exactive+ orbitrap mass spectrometer 1 Lower LLOQ with Exactive Plus Cycle time of 1.9 minutes when using a Transcend TLX-4 multiplexing system. Method robustness of approximately 6 serum injections between column changes. Ease of method set up compared to triple quadrupole MS methods. W: 1/X References 1. Shah I, James R,Barker J, Petroczi A, and Naughton D: Misleading measures in Vitamin D analysis: A novel LC-MS/MS assay to account for epimers and isobars. Nutrition Journal 211, 1:46. Acknowledgements We would like to thank Keeley Murphy for his advice and assistance. 1 All trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. For Research Use Only. Not for use in diagnostic procedures. 6 Analysis of 25-Hydroxyvitamin D In Serum Using Automated On Line Sample Preparation Technique With High Resolution Orbitrap Mass Spectrometer

7 212 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the International Standards Organization. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. This information is presented as an example of the capabilities of Thermo Fisher Scientific Inc. products. It is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. Thermo Fisher Scientific, San Jose, CA USA is ISO Certified. Africa-Other Australia Austria Belgium Canada China Denmark Europe-Other Finland/Norway/Sweden France Germany India Italy Japan Latin America Middle East Netherlands New Zealand Russia/CIS South Africa Spain Switzerland UK USA PN6368_E 6/12S

An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol

An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol An Automated Liquid Handling and Sample Preparation Workflow for LC-MS/MS Analysis of Vitamin D Metabolites, Testosterone, and Cortisol Sarah Fair, Dayana Argoti, Joseph Herman, Thermo Fisher Scientific,

More information

LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research

LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research J. Jones, J. Denbigh, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20581 Key Words SPE, SOLA,

More information

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method

Simultaneous Quantitation of 43 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Simultaneous Quantitation of 4 Drugs in Human Urine with a Dilute-and-Shoot LC-MS/MS Method Xiang He and Marta Kozak, Thermo Fisher Scientific, San Jose, CA Application Note 76 Key Words TSQ Quantum Access

More information

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs

Thermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs Thermo Scientific Prelude SPLC System FPO Making LC/MS accessible to clinical research and toxicology labs Specifically designed to make the power of LC/MS accessible Analysis of target compounds in complex

More information

Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS

Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS Application Note: 446 Selective Testosterone Analysis in Human Serum by LC-FAIMS-MS/MS Jonathan McNally and Michael Belford, Thermo Fisher Scientific, San Jose, CA James Kapron, Thermo Fisher Scientific,

More information

Fast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan

Fast, Reproducible LC-MS/MS Analysis of Dextromethorphan and Dextrorphan Fast, Reproducible LC-MS/MS Analysis of and Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 685 Key Words Accucore C18, dextromethorphan, dextrorphan, SOLA CX Abstract

More information

Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use. Confidence in Results With Data Integrity

Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use. Confidence in Results With Data Integrity Thermo Scientific ClinQuan MD Software For In Vitro Diagnostic Use Confidence in Results With Data Integrity 2 Make the World Healthier With the LC-MS Tests You Run Confidence in Test Results With Data

More information

Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS

Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS Analysis of Testosterone in Serum using LC/Triple Quadrupole MS/MS Application Note Authors M.P. George Agilent Technologies, Inc. Santa Clara, CA USA Andre Szczesniewski Agilent Technologies, Inc. Schaumburg,

More information

SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma

SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from Human Plasma SPE, LC-MS/MS Method for the Determination of Ethinyl Estradiol from uman Plasma Krishna Rao Dara, Dr. Tushar N. Mehta, Asia Pacific Center of Excellence, Thermo Fisher Scientific, Ahmedabad, India Application

More information

Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform

Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform Charles Yang, Leo Wang, Dipankar Ghosh, Jonathan Beck,

More information

Separation and Determination of Liposomal and Non-Liposomal (Free) Doxorubicin from Human Plasma by SPE and LC-MS/MS

Separation and Determination of Liposomal and Non-Liposomal (Free) Doxorubicin from Human Plasma by SPE and LC-MS/MS Separation and Determination of Liposomal and Non-Liposomal (Free) from Human Plasma by SPE and LC-MS/MS Krishna Rao Dara, Dr. Tushar N. Mehta, Tim Liddicoat, Asia Pacific Center of Excellence, Thermo

More information

UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs

UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Application Note: 439 UHPLC/MS: An Efficient Tool for Determination of Illicit Drugs Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela UHPLC System MSQ Plus MS Detector Drugs

More information

TargetQuan 3 Software. Leading the way in regulatory. POPs quantification. Bullet Bullet Bullet

TargetQuan 3 Software. Leading the way in regulatory. POPs quantification. Bullet Bullet Bullet TargetQuan 3 Software Leading the way in regulatory POPs quantification Bullet Bullet Bullet Leading the way in regulatory POPs quantification Analyse Samples Open Sequence Process Sequence Analyse Samples

More information

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz

Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische Chemie, Universitätsspital Zürich, CH-8091 Zürich, Schweiz Toxichem Krimtech 211;78(Special Issue):324 Online extraction LC-MS n method for the detection of drugs in urine, serum and heparinized plasma Daniel M. Mueller, Katharina M. Rentsch Institut für Klinische

More information

A fully quantitative research method for the analysis of lead in whole blood using the Thermo Scientific icap Q ICP-MS

A fully quantitative research method for the analysis of lead in whole blood using the Thermo Scientific icap Q ICP-MS A fully quantitative research method for the analysis of lead in whole blood using the Thermo Scientific icap Q ICP-MS Kent W. Warnken, Thermo Fisher Scientific, USA Patrick Messina, Quest Diagnostics,

More information

Thermo Scientific icap 7000 Series ICP-OES. Low cost ICP-OES analysis high quality data

Thermo Scientific icap 7000 Series ICP-OES. Low cost ICP-OES analysis high quality data Low cost ICP-OES analysis high quality data The NEW Thermo Scientific icap 7000 Series ICP-OES provides low cost multi-element analysis for measuring trace elements in a diverse sample range. The instrument

More information

Jennifer L. Simeone and Paul D. Rainville Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S INT RO DU C T ION

Jennifer L. Simeone and Paul D. Rainville Waters Corporation, Milford, MA, USA A P P L I C AT ION B E N E F I T S INT RO DU C T ION A Validated Liquid-Liquid Extraction Method with Direct Injection of Hexane for Clopidogrel in Human Plasma Using UltraPerformance Convergence Chromatography (UPC 2 ) and Xevo TQ-S Jennifer L. Simeone

More information

Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS

Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,

More information

Thermo Scientific SOLAµ SPE Plates Technical Guide. Consistent excellence. for bioanalysis

Thermo Scientific SOLAµ SPE Plates Technical Guide. Consistent excellence. for bioanalysis Thermo Scientific SOLAµ SPE Plates Technical Guide Consistent excellence for bioanalysis SOLAµ - delivering reproducible low volume extractions. Everytime! Thermo Scientific SOLAµ plates are designed for

More information

Mass and Resolution Calibration for New Triple-Stage Quadrupole Mass Spectrometers. Rob Grothe Thermo Fisher Scientific, San Jose, CA

Mass and Resolution Calibration for New Triple-Stage Quadrupole Mass Spectrometers. Rob Grothe Thermo Fisher Scientific, San Jose, CA Mass and Resolution Calibration for New Triple-Stage Quadrupole Mass Spectrometers Rob Grothe Thermo Fisher Scientific, San Jose, CA Overview Purpose: Robust and accurate calibration of mass positions

More information

Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection

Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection Determination of Pesticide Residues in Drinking Water Using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection Application Note 1097 Liu Qian, Zheng Hongguo and

More information

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping

Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small

More information

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology

Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging

More information

Ion Chromatography Assay for Chloride and Sulfate in Adenosine

Ion Chromatography Assay for Chloride and Sulfate in Adenosine Ion Chromatography Assay for and in Adenosine Lipika Basumallick and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Application ote 075 Key Words High-Pressure Ion Chromatography, Suppressed

More information

Development and Validation of Methods for Chemotherapy Drugs on the New Prelude SPLC LC-MS/MS System

Development and Validation of Methods for Chemotherapy Drugs on the New Prelude SPLC LC-MS/MS System Development and Validation of Methods for Chemotherapy Drugs on the New Prelude SPLC LC-/ System Kerry Hassell, Dayana Argoti, Sarah J. Fair and Joseph Herman Thermo Fisher Scientific, Franklin, MA, USA

More information

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY

CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in

More information

Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection

Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection Hydrogen Peroxide Detection by Ion Chromatography and Electrochemical Detection Sheetal Bhardwaj, Rong Lin, Kannan Srinivasan, and Christopher Pohl Thermo Fisher Scientific, Sunnyvale, CA, USA Overview

More information

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection

Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Rapid Separation of 25-OH- Vitamin D3 and 3-Epi-25- OH-Vitamin D3 in Human Serum using Tandem Mass Spectrometry Detection Jeff Layne, Michael McCoy, and Sky Countryman Phenomenex, Inc., 411 Madrid Ave.,

More information

Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation

Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation Craig Aurand 1, David Bell 1, Anders Fridstrom 2, and Klaus Buckendahl 2 1 Supelco, Div.

More information

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403

Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection. No. GCMS No. SSI-GCMS-1403 Gas Chromatograph Mass Spectrometer No. GCMS-1403 Analysis of Blood Alcohol by Headspace with Simultaneous GC-FID and MS Detection Introduction Determination of Blood Alcohol Content (BAC) has been a standard

More information

Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection

Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Rapid Screening Method for Illicit Drugs, Using an Advanced Solid Core UHPLC Column and UHPLC System with MS/MS Detection Derek Hillbeck, Thermo Fisher Scientific, Runcorn, UK Application Note 2999 Key

More information

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System

Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Quick and Sensitive Analysis of Multiclass Veterinary Drug Residues in Meat, Plasma, and Milk on a Q Exactive Focus LC-MS System Olaf Scheibner, Maciej Bromirski, Thermo Fisher Scientific, Bremen, Germany

More information

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water

# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system

More information

Introduction. Methods. Sample Processing

Introduction. Methods. Sample Processing PO-CON1656 High-sensitivity, high-throughput quantitation of catecholamines and metanephrine in plasma by automated WCX-SP coupled to LC/MS/MS for clinical research ASMS 2016 MP-080 Ichiro HIRANO 1, Atsuhiko

More information

Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples

Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed

More information

A Complete Solution for Method Linearity in HPLC and UHPLC

A Complete Solution for Method Linearity in HPLC and UHPLC Now sold under the Thermo Scientific brand A Complete Solution for Method Linearity in HPLC and UHPLC Frank Steiner, Fraser McLeod, Tobias Fehrenbach, and Andreas Brunner Dionex Corporation, Germering,

More information

Thermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance

Thermo Scientific SOLA SPE cartridges and plates Technical Guide. Join the revolution. unparalleled performance Thermo Scientific SOLA SPE cartridges and plates Technical Guide Join the revolution unparalleled performance Join the revolution next-generation SPE Thermo Scientific SOLA products revolutionize Solid

More information

Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS

Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS Application Note 242 Robust and Fast Analysis of Tobacco-Specific Nitrosamines by LC-MS/MS INTRODUCTION Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco products. They

More information

Determination of Tetracycline Antibiotics in Milk Using a Simple Strong Cation-Exchange SPE Cleanup Procedure and LC-MS/MS Analysis

Determination of Tetracycline Antibiotics in Milk Using a Simple Strong Cation-Exchange SPE Cleanup Procedure and LC-MS/MS Analysis Determination of Tetracycline Antibiotics in Milk Using a Simple Strong Cation-Exchange SPE Cleanup Procedure and LC-MS/MS Analysis UCT Product Numbers: CSDAU26: 2mg / 6mL SPE cartridge SLDA1ID21-3UM:

More information

Thermo Scientific SIEVE Software for Differential Expression Analysis

Thermo Scientific SIEVE Software for Differential Expression Analysis m a s s s p e c t r o m e t r y Thermo Scientific SIEVE Software for Differential Expression Analysis Automated, label-free, semi-quantitative analysis of proteins, peptides, and metabolites based on comparisons

More information

Thermo Scientific Compound Discoverer Software. A New Generation. of integrated solutions for small molecule structure ID

Thermo Scientific Compound Discoverer Software. A New Generation. of integrated solutions for small molecule structure ID Thermo Scientific Compound Discoverer Software A New Generation of integrated solutions for small molecule structure ID Transforming Small Molecule Research Solutions for Small Molecule Structural ID Thermo

More information

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS

Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS Summary: Determination of Anabolic Steroids in Horse Urine by SPE and LC-MS/MS UCT Part Numbers: CUNAX226 - Clean-Up C8+NAX, 2mg/6mL BETA-GLUC- ml Beta-Glucuronidase Enzyme, liquid form SLAQ1ID21-3UM -

More information

Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis

Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis Application Note AN844 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Page 1 Extraction of, and from Human Plasma Using EVOLUTE EXPRESS WCX Prior to LC-MS/MS Analysis Introduction Catecholamines

More information

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction

Overview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)

More information

ProteinChip Energy Absorbing Molecules (EAM)

ProteinChip Energy Absorbing Molecules (EAM) ProteinChip Energy Absorbing Molecules (EAM) Instruction Manual Catalog #C30-00001, #C30-00002, #C30-00003, #C30-00004 For technical support, call your local Bio-Rad office, or in the US, call 1-800-4BIORAD

More information

Application of Automated Data Collection to Surface-Enhanced Raman Scattering (SERS)

Application of Automated Data Collection to Surface-Enhanced Raman Scattering (SERS) Application Note: 52020 Application of Automated Data Collection to Surface-Enhanced Raman Scattering (SERS) Timothy O. Deschaines, Ph.D., Thermo Fisher Scientific, Madison, WI, USA Key Words Array Automation

More information

Improving the Cleanliness of DBS Extracts using the Ostro Pass-through 96-well Sample Preparation Plate and Single Step Method

Improving the Cleanliness of DBS Extracts using the Ostro Pass-through 96-well Sample Preparation Plate and Single Step Method Improving the Cleanliness of DBS Extracts using the stro Pass-through 96-well Sample Preparation Plate and Single Step Method Jessalynn P. Wheaton, Erin E. Chambers, and Kenneth J. Fountain APPLICATI BEEFITS

More information

AppNote 1/2014. Automated Hydrolysis, DPX Extraction and LC/MS/MS Analysis of Pain Management Drugs from Urine KEYWORDS ABSTRACT

AppNote 1/2014. Automated Hydrolysis, DPX Extraction and LC/MS/MS Analysis of Pain Management Drugs from Urine KEYWORDS ABSTRACT AppNote 1/2014 Automated Hydrolysis, DPX Extraction and LC/MS/MS Analysis of Pain Management Drugs from Urine Fred D. Foster, John R. Stuff, Edward A. Pfannkoch GERSTEL, Inc., 701 Digital Dr. Suite J,

More information

Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column

Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column Determination of Ephedra Alkaloids & Synephrine by Strong Cation Exchange SPE and LC MS/MS Detection Using a Pentafluorophenylpropyl HPLC Column UCT Part Numbers: CUBCX1HL56: high load benzenesulfonic

More information

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS

A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS A High Throughput Automated Sample Preparation and Analysis Workflow for Comprehensive Forensic Toxicology Screening using LC/MS/MS AB SCIEX QTRAP 4500 LC/MS/MS System and Gerstel, Inc. MultiPurpose Sampler

More information

Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography

Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography INTRODUCTION The analysis of soft drinks for caffeine was able to be performed using UV-Vis. The complex sample

More information

Analysis of Synthetic Cannabinoids (Spice) in Urine Utilizing HRAM

Analysis of Synthetic Cannabinoids (Spice) in Urine Utilizing HRAM Analysis of Synthetic Cannabinoids (Spice) in Urine Utilizing HRAM Kristine Van Natta Thermo Fisher Scientific History of Synthetic Cannabnoids riginally made for research in 1980s. Around 2008, they have

More information

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis

The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis The Use of Micro Flow LC Coupled to MS/MS in Veterinary Drug Residue Analysis Stephen Lock AB SCIEX Warrington (UK) Overview A rapid, robust, sensitive and specific LC-MS/MS method has been developed for

More information

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research

UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research UPLC-MS/MS Analysis of in Plasma for Clinical Research Dominic Foley and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity improves reproducibility through removal

More information

Enhancement of Linearity and Response in Charged Aerosol Detection

Enhancement of Linearity and Response in Charged Aerosol Detection Enhancement of Linearity and Response in Charged Aerosol Detection Christopher Crafts, Marc Plante, Bruce Bailey, Ian Acworth, Thermo Fisher Scientific, Chelmsford, MA, USA Overview Purpose: This work

More information

Thermo Scientific LIFECYCLE Asset & Service Management for Healthcare

Thermo Scientific LIFECYCLE Asset & Service Management for Healthcare Thermo Scientific LIFECYCLE Asset & Service Management for Healthcare HEALTHCARE SUPPLYSIDE SOLUTIONS Focused on your unique needs for managing assets, costs, and suppliers Part of Thermo Fisher Scientific

More information

Thermo Scientific Mass Spectrometric Immunoassay (MSIA)

Thermo Scientific Mass Spectrometric Immunoassay (MSIA) INSTRUCTIONS FOR USE OF PRODUCTS Thermo Scientific Mass Spectrometric Immunoassay (MSIA) Demonstration Protocol for Affinity Purification and Analysis of Human Insulin from Human Plasma Utilizing MSIA

More information

The Measurement of Vitamin D

The Measurement of Vitamin D The Measurement of Vitamin D Solving a challenging assay in the clinical research laboratory 2008 Waters Corporation Outline Introduction to Vitamin D Automated SPE approach to Vit D analysis 2008 Waters

More information

Q Exactive QuickStart Guide

Q Exactive QuickStart Guide Q Exactive QuickStart Guide About This Guide Q Exactive Tune This Q Exactive QuickStart Guide gives an introduction on setting up and using the Thermo Scientific Q Exactive mass spectrometer. This guide

More information

Simultaneous Qualitative and Quantitative Data Acquisition for Research of Diabetes Drugs

Simultaneous Qualitative and Quantitative Data Acquisition for Research of Diabetes Drugs Liquid Chromatography Mass Spectrometry SSI-LCMS-068 Simultaneous Qualitative and Quantitative Data Acquisition for Research of Diabetes Drugs LCMS-8050 Summary By utilizing the LCMS-8050 s ultrafast scan

More information

Thermo Scientific ExactFinder Software

Thermo Scientific ExactFinder Software mass spectrometry Thermo Scientific ExactFinder Software Workflow software for routine targeted and general unknown screening Unifies qualitative and quantitative high-resolution accurate mass workflows

More information

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay

Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching LC-HESI-MS/MS assay PO-CON1484E Quantitative analysis of anabolic steroids in control samples from food-producing animals using a column-switching ASMS 014 TP85 David R. Baker 1, John Warrander 1, Neil Loftus 1, Simon Hird

More information

AppNote 1/2012. Rapid Cleanup and Comprehensive Screening of Pain Management Drugs in Urine using Automated Disposable Pipette Extraction and LC-MS/MS

AppNote 1/2012. Rapid Cleanup and Comprehensive Screening of Pain Management Drugs in Urine using Automated Disposable Pipette Extraction and LC-MS/MS AppNote 1/2012 Rapid Cleanup and Comprehensive Screening of Pain Management Drugs in Urine using Automated Disposable Pipette Extraction and LC-MS/MS Oscar G. Cabrices, Fred D. Foster, John R. Stuff, Edward

More information

Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays

Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Scheduled MRM HR Workflow on the TripleTOF Systems Jenny Albanese, Christie Hunter AB SCIEX, USA Targeted quantitative

More information

Results of Using Re-washed Vials and Closures

Results of Using Re-washed Vials and Closures Results of Using Rewashed Vials and Closures Loy Shick, Thermo Fisher Scientific, Rockwood, TN, USA Brian King, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20670 Key Words Autosampler,

More information

Overview. Purpose. Methods. Results

Overview. Purpose. Methods. Results A ovel Approach to Quantify Unbound Cisplatin, Carboplatin, and xaliplatin in Human Plasma Ultrafiltrate by Measuring Platinum-DDTC Complex Using LC/M/M Min Meng, Ryan Kuntz, Al Fontanet, and Patrick K.

More information

The Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood

The Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood The Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood UCT Part Numbers: ECQUUS1015CT- Enviro-Clean 15 ml centrifuge tube with 400 mg MgSO 4 and 100 mg NaCl CUMPS2CT - Enviro-Clean

More information

Optimized SPE for UPLC/MS/MS and GC/MS/MS Determination of THC and its Metabolites in Urine and Blood

Optimized SPE for UPLC/MS/MS and GC/MS/MS Determination of THC and its Metabolites in Urine and Blood Optimized SPE for UPLC/MS/MS and GC/MS/MS Determination of THC and its Metabolites in Urine and Blood Michael S. Young, John T. Martin, Waters Corporation, 34 Maple St., Milford, MA USA APPLICATION BENEFITS

More information

Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide

Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide chromatography Thermo Scientific HyperSep Solid Phase Extraction Method Development Guide The following guide provides considerations, tips and general guidelines for developing SPE methods using the Thermo

More information

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests

Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Application Note: 386 Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Rosa Viner, Terry Zhang, Scott Peterman, and Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA,

More information

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses

New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses Technical Overview Authors Abstract Peter Stone, Thomas Glauner, Frank Kuhlmann, Tim Schlabach and Ken Miller

More information

LC-MS/MS Solutions for the Pharmaceutical and CRO Industries TSQ Series -based Solutions for Drug Discovery and Development

LC-MS/MS Solutions for the Pharmaceutical and CRO Industries TSQ Series -based Solutions for Drug Discovery and Development m a s s s p e c t r o m e t r y LC-MS/MS Solutions for the Pharmaceutical and CRO Industries TSQ Series -based Solutions for Drug Discovery and Development Part of Thermo Fisher Scientific ACCELERATING

More information

FT-NIR for Online Analysis in Polyol Production

FT-NIR for Online Analysis in Polyol Production Application Note: 51594 FT-NIR for Online Analysis in Polyol Production Key Words Acid Number Ethylene Oxide FT-NIR Hydroxyl Value Polyester Polyols Abstract Hydroxyl value and other related parameters

More information

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System

Overview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS

More information

Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column

Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Sachiki Shimizu, FUJIFILM Fine Chemicals Co., Ltd., Kanagawa, Japan Kenneth J. Fountain, Kevin Jenkins, and Yoko Tsuda,

More information

[ Care and Use Manual ]

[ Care and Use Manual ] PREP Calibration Mix DIOS Low i. Introduction Pre-packaged PREP Calibration Mixtures eliminate the need to purchase and store large quantities of the component calibration reagents, simplifying sample

More information

METHOD OF ANALYSIS. N methyl 2-pyrrolidone

METHOD OF ANALYSIS. N methyl 2-pyrrolidone METHOD OF ANALYSIS N methyl 2-pyrrolidone Center for Veterinary Medicine U.S. Food and Drug Administration 7500 Standish Place, Rockville, Maryland 20855 September 26, 2011 This method of detection for

More information

EQuan MAX Getting Connected Field Service Guide

EQuan MAX Getting Connected Field Service Guide EQuan MAX Getting Connected Field Service Guide This guide provides procedures to connect the various components for the Thermo Scientific EQuan MAX automated high-throughput LC-MS system. Contents Hardware

More information

The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes and Styrene in Olive Oil Using a Turbomatrix HS and a Clarus SQ 8 GC/MS

The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes and Styrene in Olive Oil Using a Turbomatrix HS and a Clarus SQ 8 GC/MS application Note Gas Chromatography/ Mass Spectrometry Author A. Tipler, Senior Scientist PerkinElmer, Inc. Shelton, CT 06484 USA The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes

More information

MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples

MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples Fast and Efficient Data Review, with Automatic Flagging of Outlier Results Adrian M. Taylor and Michael

More information

HRMS in Clinical Research: from Targeted Quantification to Metabolomics

HRMS in Clinical Research: from Targeted Quantification to Metabolomics A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier

More information

Increasing the Multiplexing of Protein Quantitation from 6- to 10-Plex with Reporter Ion Isotopologues

Increasing the Multiplexing of Protein Quantitation from 6- to 10-Plex with Reporter Ion Isotopologues Increasing the Multiplexing of Protein Quantitation from 6- to 1-Plex with Reporter Ion Isotopologues Rosa Viner, 1 Ryan Bomgarden, 2 Michael Blank, 1 John Rogers 2 1 Thermo Fisher Scientific, San Jose,

More information

Thermo Scientific Dionex Chromeleon 7 Chromatography Data System Software

Thermo Scientific Dionex Chromeleon 7 Chromatography Data System Software Thermo Scientific Dionex Chromeleon 7 Chromatography Data System Software Frank Tontala, Thermo Fisher Scientific, Germering, Germany Technical Note 708 Key Words Chromeleon Chromatography Data System,

More information

High-Throughput 3-D Chromatography Through Ion Exchange SPE

High-Throughput 3-D Chromatography Through Ion Exchange SPE High-Throughput 3-D Chromatography Through Ion Exchange SPE Application Note 205 Luke Roenneburg and Alan Hamstra (Gilson, Inc.) Introduction 2-dimensional (2-D) separation is the separation of a sample

More information

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System

A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System A Generic LC-MS Method for the Analysis of Multiple of Drug of Abuse Classes with the Thermo Scientific Exactive TM System Kent Johnson Fortes lab, Wilsonville Oregon List of drug of abuse candidates for

More information

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract

Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate

More information

ApplicationNOTE Introduction

ApplicationNOTE Introduction Introduction Honey, like other foods, is subject to strict quality control measures before it can be sold commercially. It is a natural product, produced solely by bees from flower and tree pollen. Human

More information

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS

Therapeutic Drug Monitoring of Antiretroviral Drugs with HPLC-MS Therapeutic Drug Monitoring of Antiretroviral Drugs with PLC-M Ursula Gutteck-Amsler, Katharina M. Rentsch Abstract Prospective and retrospective studies have provided some evidence of the clinical and

More information

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC

SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6- -NALTREXOL IN SERUM BY HPLC Katja SÄRKKÄ, Kari ARINIEMI, Pirjo LILLSUNDE Laboratory of Substance Abuse, National Public Health Institute Manerheimintie,

More information

WATERS QUANTITATIVE ANALYSIS solutions

WATERS QUANTITATIVE ANALYSIS solutions More sensitivity. More speed. What more can you ask for in a complete quantitative analysis solution? WATERS QUANTITATIVE ANALYSIS solutions THE CHALLENGE OF QUANTITATIVE ANALYSIS Tandem mass spectrometry,

More information

Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged Aerosol Detection

Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged Aerosol Detection Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged Aerosol Detection Mark Tracy, Xiaodong Liu, and Ian Acworth, Thermo Fisher Scientific, Sunnyvale, CA,

More information

Detection of Adulterant Seed Oils in Extra Virgin Olive Oils by LC-MS and Principal Components Analysis

Detection of Adulterant Seed Oils in Extra Virgin Olive Oils by LC-MS and Principal Components Analysis Detection of Adulterant Seed Oils in Extra Virgin Olive Oils by LC-MS and Principal Components Analysis Nadia Pace AB SCIEX Concord, Ontario (Canada) Overview Various oils were analyzed using LC-MS/MS

More information

Optimizing the Post-implementation Monitoring of a LC-MS/MS Method

Optimizing the Post-implementation Monitoring of a LC-MS/MS Method Optimizing the Post-implementation Monitoring of a LC-MS/MS Method Julianne Cook Botelho, PhD Centers for Disease Control and Prevention Atlanta, GA AACC- Mass Spectrometry in the Clinical Lab: Best Practice

More information

Method Development of LC-MS/MS Analysis of Aminoglycoside Drugs: Challenges and Solutions

Method Development of LC-MS/MS Analysis of Aminoglycoside Drugs: Challenges and Solutions Method Development of LC-MS/MS Analysis of Aminoglycoside Drugs: Challenges and Solutions authors Angela (Qi) Shen, Ling Morgan, Marcele L. Barroso, and Xin Zhang; Tandem Labs Tuyen Nguyen; Sepracor Inc.

More information

HPLC Analysis of Acetaminophen Tablets with Waters Alliance and Agilent Supplies

HPLC Analysis of Acetaminophen Tablets with Waters Alliance and Agilent Supplies HPLC Analysis of Acetaminophen Tablets with Waters Alliance and Agilent Supplies Application Note Small Molecule Pharmaceuticals Authors Jignesh Shah, Tiantian Li, and Anil Sharma Agilent Technologies,

More information

Comparison of Supercritical Fluid Extraction (SFE) and Pressurized Solvent Extraction (PSE) for the Recovery of gamma-tocopherols in Walnut Oil

Comparison of Supercritical Fluid Extraction (SFE) and Pressurized Solvent Extraction (PSE) for the Recovery of gamma-tocopherols in Walnut Oil Comparison of Supercritical Fluid Extraction (SFE) and Pressurized Solvent Extraction (PSE) for the Recovery of gamma-tocopherols in Walnut Oil Jeff Wright and Thomas DePhillipo Waters Corporation, Milford,

More information

Thermo Scientific Qtegra Intelligent Scientific Data Solution (ISDS) Software for 21 CFR Part 11 Compliant Laboratories

Thermo Scientific Qtegra Intelligent Scientific Data Solution (ISDS) Software for 21 CFR Part 11 Compliant Laboratories Thermo Scientific Qtegra Intelligent Scientific Data Solution (ISDS) Software for 21 CFR Part 11 Compliant Laboratories Technical Note 43106 Key Words Compliance, Electronic Records, 21 CFR Part 11 Goal

More information

Pesticide Analysis by Mass Spectrometry

Pesticide Analysis by Mass Spectrometry Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine

More information

Thermo Scientific ConFlo IV Universal Interface

Thermo Scientific ConFlo IV Universal Interface m a s s s p e c t r o m e t r y Thermo Scientific ConFlo IV Universal Interface The Universal Interface Part of Thermo Fisher Scientific ConFlo IV Universal Interface The Universal Interface The development

More information