Growth Kinetics of Human Mesenchymal Stem Cells from Bone Marrow and Umbilical Cord Blood
|
|
- Alison Ferguson
- 7 years ago
- Views:
Transcription
1 Brief Communication Acta Haematol 2004;112: DOI: / Received: December 16, 2002 Accepted after revision: May 12, 2004 Growth Kinetics of Human Mesenchymal Stem Cells from Bone Marrow and Umbilical Cord Blood Tae Jin Kang a Jung-Eun Yeom a Hye Jung Lee a Seung Hye Rho b Hoon Han c Gue-Tae Chae a Departments of a Pathology and b Microbiology, College of Medicine, The Catholic University of Korea, c Research Institute of Biotechnology, HistoStem Corporation, Seoul, Republic of Korea Bone marrow (BM) is a rich source of human mesenchymal stem cells (MSCs). MSCs were first identified by Friedenstein et al. [1], who demonstrated that when BM is plated in fetal bovine serum (FBS)-containing medium, colonies of adherent fibroblast-like cells develop that differentiate into bone and adipocytes. The cells were referred to as MSCs or mesenchymal progenitor cells, because of their ability to differentiate into a variety of nonhematopoietic cells. Since then, several investigators have shown that these cells can also differentiate into chondrocytes, adipocytes, osteocytes and myocytes [2 5]. MSCs have recently been shown to have the potential to serve as effective vehicles for cell therapy, gene therapy and immunotherapy [6, 7]. Umbilical cord blood (UCB) has been utilized for human hematopoietic stem cell transplantation as an alternative source to BM [8]. MSCs are adult stem cells, present in most tissues. Although the presence of hematopoietic stem cells in UCB is well known, that of MSCs has not been fully evaluated. However, recent research has shown that UCB is also a source of human MSCs [9]. Nevertheless, the presence of MSCs in UCB is a subject of debate among researchers [10, 11]. The goal of the current study was to determine the growth kinetics of purified human MSCs during subcultivation, and to compare the growth kinetics in cells from BM and UCB. All samples were obtained with the written, informed consent of the donors in accordance with the requirements of the hospital ethics committee. Cord blood harvests (n = 67) were a gift by HistoStem Corp. (Seoul, Korea), and BM samples (n = 7) were from the Catholic Hematopoietic Stem Cell Bank (Catholic Medical Center, Seoul, Korea). The age of cord blood donors ranged between 22 and 35 years, and the median gestational age was 29 years. Cord blood and BM were separated into a low-density fraction (Histo-Paque-1077, Amersham Pharmacia), and mononuclear cells were washed, suspended in culture medium [Dulbecco s modified Eagle s medium containing 20% FBS (Hyclone) and 10% streptomycin-penicillin (Gibco BRL, N.Y., USA)] and seeded at a concentration of 1! 10 8 cells/cm 2. Cultures were maintained at 37 C in a humidified atmosphere containing 5% CO 2 with a change of culture medium every 7 days until the fibroblast-like cells at the base of the flask reached confluence. On reaching confluence, the adherent cells were resuspended using 0.25% trypsin-edta and reseeded at 1! 10 6 cells/flask (first passage). These cells were incubated again until confluence, and were once again trypsinized and reseeded at 1! 10 6 cells/flask (second passage). At the end of the second passage, when the cells reached confluence, they were trypsinized and either cryopreserved or used immediately. ABC Fax karger@karger.ch S. Karger AG, Basel /04/ $21.00/0 Accessible online at: Dr. Gue-Tae Chae Department of Pathology, College of Medicine, The Catholic University of Korea 505 Banpo-Dong, Seocho-Gu Seoul, (Republic of Korea) Tel , Fax , guetae@catholic.ac.kr
2 Fig. 1. A Growth curves of human MSC cultures from UCB at passages 3, 4 and 5 (P3, P4 and P5, respectively). At each passage, cells were plated in 6-well plates at 4! 10 3 cells/well, and medium was changed twice weekly. Triplicate cultures were harvested for calculation of cell number. The results represent the mean cell number B SD of 3 preparations. * p! 0.05 compared to passage 3 culture. B Comparison of growth curves of human MSC cultures from UCB (n = 3) and BM (n = 3) at passage 3. MSCs from UCB and BM, respectively, were plated in 6-well plates at 4! 10 3 cells/well. Triplicate cultures were harvested for calculation of cell number. The results represent the mean cell number B SD from one representative experiment. * p! 0.05 compared to MSCs from UCB. Statistical analyses were performed using Student s t test. To determine growth kinetics of MSCs from UCB and BM, MSCs were seeded in 6-well plates at 4! 10 3 cells/ well. Every 2 days for 12 days, triplicate cultures were trypsinized and counted on a hemocytometer. For the measurement of growth kinetics of serially passaged cultures, at each passage, MSCs were harvested for calculation of cell number, by the same method. Growth kinetics of MSCs from UCB were measured at passages 3, 4 and 5, and compared with those of MSCs from BM. Cells were allowed to divide for 12 days, with medium changes occurring twice weekly. Figure 1 shows the growth curves obtained at passages 3, 4 and 5 and illustrates the similarities and differences of MSC growth characteristics between UCB and BM. At each passage, MSC growth curves show an initial lag phase of 2 4 days. This was followed by a log phase in which the MSCs divided at exponential rates for 6 10 days, depending on the passage from which the cells were derived. With increasing passage number, the MSC growth rates were slower and the number of cells generated by the end of 12 days in culture was reduced (fig. 1A). MSCs from BM show the same results (data not shown). MSC cultures from BM grew at faster rates and generated significantly more cells by the end of the 12-day growth period as compared to cultures from UCB (fig. 1B). In contrast to our finding that MSCs could be isolated from UCB, it was reported recently that it was not possible to isolate MSCs from UCB [10, 11]. It is likely that this discrepancy is due to factors such as the difference in culture conditions and the very low frequency of MSCs in UCB, among others. It might be significant that the number of total mononuclear cells varies in different cord blood units. The total number of mononuclear cells in the cord blood units that were used in this study ranged from 0.5! 10 8 to 5! MSCs were isolated in the cord blood units that averaged a total of 4! 10 8 mononuclear cells. However, the cord blood units with 1.2! 10 8 mononuclear cells showed poor colony formation, so we could not find MSCs in Growth Kinetics of Human Mesenchymal Stem Cells Acta Haematol 2004;112:
3 Fig. 2. Photomicrographs showing MSCs from UCB. A Primary cultures of MSCs contain a heterogeneous population. B Confluent UCB-derived MSCs, displaying homogeneous fibroblastoid cells. C, D Adipogenic differentiation is visually apparent by the accumulation of lipid vacuoles (C) that stained with oil red O (D) after 14 days of induction. Original magnification! Acta Haematol 2004;112: Kang/Yeom/Lee/Rho/Han/Chae
4 them. Therefore, we hypothesize that the total number of mononuclear cells in a cord blood unit is one of the important factors for the successful isolation of MSCs. We sought to establish the morphological differences of MSCs derived from cord blood in a time-dependent manner. Five of 67 cultured UCB harvests had fibroblastlike cells morphologically distinguishable from other adherent cells. However, in contrast to UCB, all our BM samples (n = 7) produced MSCs. MSCs were cultured from the Ficoll fraction of mononuclear cells from UCB in an enriched medium containing 20% FBS. UCBderived mononuclear cells, when in culture, gave rise to adherent cells which exhibited fibroblast-like morphology and remained as a monolayer in vitro. Primary cultures of MSCs contained a heterogeneous population (fig. 2A). After 28 days, MSC culture appeared morphologically as a homogenous population of fibroblastoid cells (fig. 2B). UCB-derived adherent cells expressed CD13, CD29, CD44, CD59, CD90 and CD105 (SH2), but did not express CD14, CD34 and CD45, found on hematopoietic precursors. The lack of expression of CD14, CD34 and CD45 suggested that cultures were depleted of hematopoietic cells. To confirm that the isolated cells were MSCs, they were cultured in adipogenic media and differentiated into adipocytes. At the end of passage 3, when UCB-derived MSCs were treated with adipogenic media, cells were differentiated into adipocytes, which was apparent by the accumulation of lipid-containing vacuoles (fig. 2C) as detected by microscopy after staining with oil red O (fig. 2D). Cells containing lipid vacuoles were gradually observed after 1 week. Our results suggest that although it is difficult to isolate MSCs from UCB and while MSCs from UCB proliferate slowly in vitro in comparison to those from BM, UCB could be regarded as a source of MSCs for experimental and clinical needs. Further studies are needed to establish rapid and easy methods for isolation of MSCs from UCB. Acknowledgment This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (01- PJ10-PG8-01EC ). References 1 Friedenstein AJ, Gorskaja JF, Kalugina NN: Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 1976;4: Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP: Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 1997;64: Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science 1999;284: Reyes M, Lund T, Lenvik T, Aguiar D, Koodie L, Verfaillie CM: Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood 2001;98: Nakamura T, Shiojima S, Hirai Y, Iwama T, Tsuruzoe N, Hirasawa A, Katsuma S, Tsujimoto G: Temporal gene expression changes during adipogenesis in human mesenchymal stem cells. Biochem Biophys Res Commun 2003; 303: Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissue. Science 1997;276: Jorgensen C, Djouad F, Apparailly F, Noel D: Engineering mesenchymal stem cells for immunotherapy. Gene Ther 2003;10: Lewis ID, Almeida-Porada G, Du J, Lemischka IR, Moore KA, Zanjani ED, Verfaillie CM: Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood 2001;97: Erices A, Conget P, Minguell JJ: Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 2000;109: Mareschi K, Biasin E, Piacibello W, Aglietta M, Madon E, Fagioli F: Isolation of human mesenchymal stem cells: Bone marrow versus umbilical cord blood. Haematologica 2001;86: Wexler SA, Donaldson C, Denning-Kendall P, Rice C, Bradley B, Hows JM: Adult bone marrow is a rich source of human mesenchymal stem cells but umbilical cord and mobilized adult blood are not. Br J Haematol 2003;121: Growth Kinetics of Human Mesenchymal Stem Cells Acta Haematol 2004;112:
5
Basic Science in Medicine
Medical Journal of th e Islamic Republic of Iran Volume 18 Number 3 Fall 1383 November 2004 Basic Science in Medicine ] EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN
More informationHUMAN MESENCHYMAL STEM CELLS AS BASIC TOOLS FOR TISSUE ENGINEERING: ISOLATION AND CULTURE
HUMAN MESENCHYMAL STEM CELLS AS BASIC TOOLS FOR TISSUE ENGINEERING: ISOLATION AND CULTURE MONICA NEAGU*, ERIKA SUCIU**, VALENTIN ORDODI**, VIRGIL PĂUNESCU** *Department of Biophysics and Medical Informatics,
More informationMultipotent mesenchymal stromal cells. original research report
Parameters that influence the isolation of multipotent mesenchymal stromal cells from human umbilical cord blood Attiyeh Vasaghi a, Atefeh Dehghani a, Zeinab Khademalhosseini a, Mohsen Khosravi Maharlooei
More informationEquine Stem Cells. Sources, Processing, Expansion, Storage and Shipping
Equine Stem Cells Sources, Processing, Expansion, Storage and Shipping Sean Owens, DVM, Diplomate ACVP Medical Director, UC Davis Veterinary Regenerative Medicine Laboratory What is a Stem Cell and What
More information3D Cell Culture mimsys G
3D Cell Culture mimsys G xeno-free & nutrient permeable hydrogel for 3D cell culture mimsys G is a xeno-free and non-immunogenic, easy to handle hydrogel for cell encapsulation in 3D experiments in vitro
More informationChondrogenical Differentiation of Umbilical Cord Lining. Stem Cells
Chondrogenical Differentiation of Umbilical Cord Lining Stem Cells He Xi, Masilamani Jeyakumar, Phan Toan Thang Bioengineering, National University of Bioengineering Abstract Cartilage defects, such as
More informationOriginalArticle. Critical Parameters for the Isolation of Mesenchymal Stem Cells from Umbilical Cord Blood
OriginalArticle Critical Parameters for the Isolation of Mesenchymal Stem Cells from Umbilical Cord Blood KAREN BIEBACK, SUSANNE KERN, HARALD KLÜTER, HERMANN EICHLER Institute of Transfusion Medicine and
More informationComparison of Mesenchymal Stem Cells derived from Amniotic Fluid and Umbilical Cord
Academy of Sustainable Development and Technology. Comparison of Mesenchymal Stem Cells derived from Amniotic Fluid and Umbilical Cord Master of Science Thesis (30hp) Flemmingsberg, Sweden 2011 Åsa Ekblad
More informationMesenchymal Stem Cells
Mesenchymal Stem Cells Instruction Manual Product Size Catalog Number from Bone Marrow (hmsc-bm) from Umbilical Cord Matrix (hmsc-uc) from Adipose Tissue (hmsc-at) 500,000 cryopreserved cells 500,000 proliferating
More informationTECHNICAL MANUAL VERSION 2.2.0. Enumeration, Expansion, and Differentiation of Human Mesenchymal Progenitor Cells Using MesenCult
TECHNICAL MANUAL VERSION 2.2.0 Enumeration, Expansion, and Differentiation of Human Mesenchymal Progenitor Cells Using MesenCult Table of Contents 1.0 Introduction...1 2.0 Materials...2 2.1 Product Description...2
More informationRole Of Mitochondria In Mesenchymal Stem Cells
Role Of Mitochondria In Mesenchymal Stem Cells Roman Eliseev, MD, PhD, Tamara Raymond, Regis O'Keefe, MD, PhD. University of Rochester, Rochester, NY, USA. Disclosures: R. Eliseev: None. T. Raymond: None.
More informationHuman Umbilical Cord-derived Multipotent Mesenchymal Stromal Cells (huc-msc) Handling Instructions
Human Umbilical Cord-derived Multipotent Mesenchymal Stromal Cells (huc-msc) Order No.: 19401-005, 19401-010 Handling Instructions For preclinical ex vivo use. Not intended for therapeutic use. Table of
More informationHow To Expand Hematopoietic Stem Cells
Purification and Expansion of Hematopoietic Stem Cells Based on Proteins Expressed by a Novel Stromal Cell Population Our bodies are constantly killing old, nonfunctional, and unneeded cells and making
More informationProcessing & Utilization of Cord Blood for Transplant
Processing & Utilization of Cord Blood for Transplant 2010-Jan Jan-15 Nicole L. Prokopishyn, PhD HPC Processing Laboratory Director Calgary Laboratory Services Overview Cord Blood Processing Pre-Freeze
More informationBioResearch. Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research
BioResearch Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research BioResearch Hematopoietic and Immune Cell Products Essential Tools for Hematopoietic Research Working with
More information1 Materials and methods. 1.1 Materials
Vol. 44 No. 5 SCIENCE IN CHINA (Series C) October 2001 Expansion of CD34 + cells from human umbilical cord blood by FL and/or TPO gene transfected human marrow stromal cell lines ZHANG Yi (Æ Ê), TANG Peixian
More informationPoietics human mesenchymal stem cells Instructions for use
Poietics human mesenchymal stem cells Instructions for use www.lonza.com U.S. Scientific Support: 800-521-0390 scientific.support@lonza.com EU/ROW Scientific Support: +49-221-99199-400 scientific.support.eu@lonza.com
More informationNewborn Stem Cells from Cord Blood and the Brain: Repairing Injury and Improving Function
BACKROUNDER: Newborn Stem Cells from Cord Blood and the Brain: Repairing Injury and Improving Function Introduction WITH ONGOING DEVELOPMENTS IN RESEARCH, it is estimated that 1 in every 3 people may benefit
More informationCytotherapy (2005) Vol. 7, No. 4, 368/373
Cytotherapy (2005) Vol. 7, No. 4, 368/373 A 37-year-old spinal cord-inured female patient, transplanted of multipotent stem cells from human UC blood, with improved sensory perception and mobility, both
More informationThe Therapeutic Potential of Human Umbilical Cord Blood Transplantation for Neonatal Hypoxic-Ischemic Brain Injury and Ischemic Stroke
The Therapeutic Potential of Human Umbilical Cord Blood Transplantation for Neonatal Hypoxic-Ischemic Brain Injury and Ischemic Stroke a,b* b,c a a b b b b a b a b c 430 Wang et al. Acta Med. Okayama Vol.
More informationMesenchymal progenitor cells in human umbilical cord blood
British Journal of Haematology 2000, 109, 235±242 Mesenchymal progenitor cells in human umbilical cord blood ALEJANDRO ERICES, 1 PAULETTE CONGET 1 and JOSEÂ J. MINGUELL 1,2 1 Unidad de BiologõÂa Celular,
More informationHuman Placenta MSC in Placental Analysis
Chang ARTICLE Dong LI et al Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation Chang Dong LI 1, Wei Yuan ZHANG 1,*, He Lian LI 2, XiaoXia
More informationOsteoblast Differentiation and Mineralization
Osteoblast Differentiation and Mineralization Application Note Background Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. They develop from mesenchymal precursors.
More informationCurrent Issues in Stem Cell Technologies. Lance D. Trainor, MD OneBlood, Inc.
Current Issues in Stem Cell Technologies Lance D. Trainor, MD OneBlood, Inc. Objective: The big picture of stem cell therapy Outline: Relevant definitions History of Stem Cell Therapy Hematopoietic Stem
More informationChapter 16 Reproductive Technology, Gene Therapy, and Stem Cells (modified)
Chapter 16 Reproductive Technology, Gene Therapy, and Stem Cells (modified) Assisted Reproductive Technologies (ART) Artificial insemination (AI) In vitro fertilization (IVF) Gamete intrafallopian transfer
More informationCord Blood Re-imagined The use of cord blood stem cells for clinical applications and medical innovations
GE Healthcare Proceedings of the Symposium Cord Blood Re-imagined The use of cord blood stem cells for clinical applications and medical innovations 12 th Annual Meeting of the International Society for
More informationIsolation of multipotent mesenchymal stem cells from umbilical cord blood
HEMATOPOIESIS Isolation of multipotent mesenchymal stem cells from umbilical cord blood Oscar K. Lee, Tom K. Kuo, Wei-Ming Chen, Kuan-Der Lee, Shie-Liang Hsieh, and Tain-Hsiung Chen It is well accepted
More informationSummary of Act for Appropriate Provision of Hematopoietic Stem Cells to be Used in Transplantations
Summary of Act for Appropriate Provision of Hematopoietic Stem Cells to be Used in Transplantations Basic principles are established and the responsibilities of the national government are clarified in
More informationStem cells possess 2 main characteristics: Sources of pluripotent stem cells: -Long-term self renewal. -The inner cell mass of the blastocyst.
Stem cells possess 2 main characteristics: -Long-term self renewal. - They give rise to all types of differentiate cells. Sources of pluripotent stem cells: -The inner cell mass of the blastocyst. - Fetal
More informationReview. Cord blood as an alternative source of haemopoietic stem cells. J. Hows, A. Nicol, P. Denning-Kendall, C. Donaldson, M. Nieda & B.
Annals of Oncology 7 (Suppi 2): 47-51, 1996. O 1996 Kluwer Academic Publishers. Printed in the Netherlands. Review Cord blood as an alternative source of haemopoietic stem cells J. Hows, A. Nicol, P. Denning-Kendall,
More informationProtocols for Adipogenic Differentiation Assays for Characterization of Adipose Stromal Cells (ASC)
Protocols for Adipogenic Differentiation Assays for Characterization of Adipose Stromal Cells (ASC) Purpose: To characterize the adipogenic differentiation capabilities of adipose stromal cells. Overview:
More informationCord Blood, Cord Life
投 稿 類 別 : 英 文 寫 作 類 篇 名 : Cord Blood, Cord Life 作 者 : 詹 苡 廷 國 立 台 中 家 商 應 用 外 語 科 三 年 二 班 張 詠 婷 國 立 台 中 家 商 應 用 外 語 科 三 年 二 班 賴 雅 云 國 立 台 中 家 商 應 用 外 語 科 三 年 二 班 指 導 老 師 : 廖 明 珠 Abstract In the past few
More informationThe immunomodulatory activity of human umbilical cord bloodderived mesenchymal stem cells in vitro
IMMUNOLOGY ORIGINAL ARTICLE The immunomodulatory activity of human umbilical cord bloodderived mesenchymal stem cells in vitro Meng Wang, 1 Yuan Yang, 2 Dongming Yang, 1 Fei Luo, 1 Wenjie Liang, 1 Shuquan
More informationHuman Adult Mesothelial Cell Manual
Human Adult Mesothelial Cell Manual INSTRUCTION MANUAL ZBM0025.01 SHIPPING CONDITIONS Human Adult Mesothelial Cells Orders are delivered via Federal Express courier. All US and Canada orders are shipped
More informationHARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs)
HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) OBJECTIVE: can be cryopreserved in a liquid nitrogen (LN 2 ) freezer for long-term storage. This Standard Operating Procedure (SOP)
More informationBiochemical and Biophysical Research Communications 321 (2004) 102 108
Biochemical and Biophysical Research Communications 321 (2004) 102 108 BBRC www.elsevier.com/locate/ybbrc In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells Eun
More informationCultivation and Cryopreservation of Cord Tissue MSCs with Cord Blood AB Plasma
Cultivation and Cryopreservation of Cord Tissue MSCs with Cord Blood AB Plasma Manasi Talwadekar, Darshana Kadekar, Sonal Rangole, Nikhat Firdaus Khan, Vaijayanti Kale and Lalita Limaye* Stem Cell Laboratory,
More informationHuman Umbilical Cord Blood CD34 + Progenitor Cell Care Manual
Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.03 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+ Progenitor Cells, cryopreserved Cryopreserved human umbilical
More informationImproved Ex Vivo Expansion of Functional CD34 + Cells Using Stemline II Hematopoietic Stem Cell Expansion Medium
Improved Ex Vivo Expansion of Functional CD34 + Cells Using Stemline II Hematopoietic Stem Cell Expansion Medium Daniel W. Allison, 1 Stacy L. Leugers, 1 Barry J. Pronold, 2 Gary Van Zant, 2 Jenny A. Harrington,
More informationSTEM CELLS FROM THE UMBLICAL CORD BLOOD AND UMBLICAL CORD TISSUE
STEM CELLS FROM THE UMBLICAL CORD BLOOD AND UMBLICAL CORD TISSUE What are Stem Cells? Stem cells are the basic building blocks of all the cells, tissues and organs in the human body. The role of the stem
More informationCore Principles in Cellular Therapy, 3rd edition
Brochure More information from http://www.researchandmarkets.com/reports/3146051/ Core Principles in Cellular Therapy, 3rd edition Description: This handy text provides a focused look at hematopoietic
More informationThe Impact of Maternal, Neonatal and Collections factors on the TNC count of an Umbilical Cord Blood Donation
The Impact of Maternal, Neonatal and Collections factors on the TNC count of an Umbilical Cord Blood Donation By: NHS Cord Blood Bank (Colindale Laboratory) Date: 26 th September 2014 NHS insert Cord your
More informationHuman Mesenchymal Stem Cell Functional Identification Kit
Human Mesenchymal Stem Cell Functional Identification Kit Catalog Number SC006 Reagents for the identification of human bone marrow-derived stem cells (BMSCs)/mesenchymal stem cells (MSCs) by in vitro
More informationguides BIOLOGY OF AGING STEM CELLS An introduction to aging science brought to you by the American Federation for Aging Research
infoaging guides BIOLOGY OF AGING STEM CELLS An introduction to aging science brought to you by the American Federation for Aging Research WHAT ARE STEM CELLS? Stem cells are cells that, in cell cultures
More informationSaving umbilical blood from newborn infants: the clinical potential, economics and ethics
Saving umbilical blood from newborn infants: the clinical potential, economics and ethics Abstract: Umbilical cord blood is the blood remaining in the placenta and umbilical cord after childbirth. It had
More informationCD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors
CD34+ CD45- Cells Isolated from Human Blood Have Molecular and Functional Characteristics of Vascular Endothelial Progenitors Yajuan Jiang 1, Alexander M. Tsankov 2, Alexander Meissner 2 and David W. O
More informationTransmedcon 2014. Ahmedabad, Gujarat November 14-16, 2014
FACT Accreditation for Cord Blood Banks and Cellular Therapy Nadim Mahmud, MD, PhD Associate Professor of Medicine Division of Hematology/Oncology Director, Hospital Stem Cell Laboratory Blood & Marrow
More informationUmbilical Cord Blood Stem Cells Current Status & Future Potential
Umbilical Cord Blood Stem Cells Current Status & Future Potential Natasha Ali Assistant Professor Haematology Department of Pathology & Laboratory Medicine/Oncology The Aga Khan University Email: natasha.ali@aku.edu
More informationFollicle Dermal Papilla Cell
Follicle Dermal Papilla Cell Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product Description
More informationHuman Adult Stem Cell Manual Differentiation protocols for human adipose-derived adult stem cells
Human Adult Stem Cell Manual Differentiation protocols for human adipose-derived adult stem cells INSTRUCTION MANUAL ZBM0015.01 SHIPPING CONDITIONS Human Adult Stem Cells (ASC) Orders are delivered via
More informationCPT Codes for Bone Marrow Transplant January 2015 James L. Gajewski, MD
The blood and marrow transplant field has 15 dedicated CPT codes. These CPT codes can be categorized into three groups: 1. Collection Codes 2. Cell Processing Codes 3. Cell Infusion Codes Collection Codes
More informationCord derived MSC-Like Placenta derived Membranes- Matrix- Cells- MSC-like,
Bank Public/Pvt Bank other cells Ship and distribute Collect and process other samples Support development of novel uses One cord Blood Unit Non stem cells RBC s Plateletes T B Monocytes NK cells Stem
More information5 Frequently Asked Questions About Adult Stem Cell Research
5 Frequently Asked Questions About Adult Stem Cell Research Stem cells are often referred to in the sociopolitical realm with some level of controversy and beyond that, some level of confusion. Many researchers
More informationSaving Your Baby s Cord Blood
Saving Your Baby s Cord Blood An Important Pregnancy Decision 1-888-CORD BLOOD HEALTHY FUTURES ARE Born at CBR For every parent who wishes they could do more to protect their family s health, there s Cord
More informationAAGPs TM Anti-Aging Glyco Peptides. Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule
AAGPs TM Anti-Aging Glyco Peptides Enhancing Cell, Tissue and Organ Integrity Molecular and biological attributes of lead AAGP molecule 1 Acknowledgements This presentation was prepared by Dr. Samer Hussein
More informationStem cells and cellular therapies: from research to clinic. ns,, PhD Faculty of Biology University of Latvia
Stem cells and cellular therapies: from research to clinic Janis Ancans ns,, PhD Faculty of Biology University of Latvia Our cells Human body originates from one stem cell Conventional drugs Long history
More informationyour complete stem cell bank
your complete stem cell bank HYDERABAD - 88985 000 888, WARANGAL - 8297 256 777 VISAKHAPATNAM - 7799 990 774 VIJAYAWADA AND GUNTUR - 7799 990 771 NELLORE - 7799 990 772, KADAPA - 8297 256 700 RAJAHMUNDRY
More informationDo you have anything to add? If so, I d love to hear from you! Jessica Robinson Conference Manager Life Sciences @jessbiopharma
1 Who is the most influential figure in cord blood around the world? What is the biggest challenge to overcome in the use of cord blood as a source of stem cells? We asked 10 leading experts in the cord
More informationThe Prospect of Stem Cell Therapy in Multiple Sclerosis. Multiple sclerosis is a multifocal inflammatory disease of the central
The Prospect of Stem Cell Therapy in Multiple Sclerosis Multiple sclerosis is a multifocal inflammatory disease of the central nervous system that generally affects young individuals, causing paralysis
More informationAutomated and Standardized Counting of CFU Assays of Human Hematopoietic Cells
Automated and Standardized Counting of CFU Assays of Human Hematopoietic Cells 2 Automated and Standardized Colony Counting Table of Contents 4 Standardized Counting of Colony-Forming Unit Assays (CFU)
More informationPROTOCOL. Immunocytochemistry (ICC) MATERIALS AND EQUIPMENT REQUIRED
PROTOCOL Immunocytochemistry (ICC) 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 11-07 MATERIALS AND EQUIPMENT REQUIRED Materials: MitoSciences primary monoclonal antibody/antibodies Fluorophore-conjugated
More informationNewborn Stem Cells from Cord Blood and the Brain: Repairing Injury and Improving Function
BACKROUNDER: Newborn Stem Cells from Cord Blood and the Brain: Repairing Injury and Improving Function Introduction WITH ONGOING DEVELOPMENTS IN SCIENCE, researchers estimate that 1 in every 3 people may
More informationTecan Symposium Biologics-From Benchtop to Production
Tecan Symposium Biologics-From Benchtop to Production Cellular products to improve haemopoietic stem cell transplants David Haylock Australian Stem cell Centre Haemopoietic stem cell transplantation Bone
More informationUMBILICAL CORD BLOOD, STEM CELL BANKING
UMBILICAL CORD BLOOD, STEM CELL BANKING Dr.Sharad Jain MD Blood Transfusion officer, & I/C Transfusion Medicine NSCB Medical College. Jabalpur.MP. Introduction: Every parent during childbirth DREAMS the
More informationFuture Health Technologies. From Academia to Industry The Stem Cell Challenge. Roger J. Dainty MBE FIScT UK Managing Director
Future Health Technologies From Academia to Industry The Stem Cell Challenge Roger J. Dainty MBE FIScT UK Managing Director How Did I Get Here? Working as a Research Medical Technician (Birmingham University
More informationCell Phone Radiation Effects on Cancer. Tyler Barkich Central Catholic High School Grade 11
Cell Phone Radiation Effects on Cancer Tyler Barkich Central Catholic High School Grade 11 Cancer Overview Cancer cells are cells that grow and divide at an irregular, unregulated pace. Apoptosis does
More informationSTEMCELL Quality Control Kits
STEMCELL Quality Control Kits i Table of Contents 1.0 Introduction...1 2.0 Thawing Cells, Plating and Colony Enumeration...2 2.1 Supplies and reagents included in the QC kit...2 2.2 Additional reagents
More informationBioArchive Freezing Curve for Cord Blood
BioArchive Freezing Curve for Cord Blood Determination of the Specific Freezing in the BioArchive System to Provide the Highest Viability of Cord Blood MNC as Measured by -C Assays Introduction Freezing
More informationCellartis Protocol Culturing of hes Cells
Cellartis Protocol Culturing of hes Cells This material was cultured and frozen using Cellartis protocols. WiCell recommends that stem cells should be thawed and established in the conditions in which
More informationDonation of Umbilical Cord Blood. A precious life offer. for everyone!
Donation of Umbilical Cord Blood A precious life offer for everyone! Donation of Umbilical Cord Blood A precious life offer for everyone! Page 3 Hellenic Cord Blood Bank Page 4 Haematopoietic Progenitor
More informationTERUMO Corporation Business Strategy Conference
TERUMO Corporation Business Strategy Conference THERAPEUTIC SYSTEMS & CELL PROCESSING MARK FLOWER GLOBAL MARKETING December 5, 2011 Regenerative Medicine, Cell Therapy, & Cell Processing Regenerative Medicine
More informationEnumeration and Viability of Nucleated Cells from Bone Marrow, Cord Blood, and Mobilized Peripheral Blood
Application Note Primary Cell Analysis Enumeration and Viability of Nucleated Cells from Bone Marrow, Cord Blood, and Mobilized Peripheral Blood Cell Viability Introduction: Bone Marrow, Cord Blood, Stem
More informationStem Cell Treatment for you in 2015: A Reference for Physicians, Patients and a Review of International Stem Cell Treatment Centers
International Journal of Sciences: Basic and Applied Research (IJSBAR) ISSN 2307-4531 (Print & Online) http://gssrr.org/index.php?journal=journalofbasicandapplied ---------------------------------------------------------------------------------------------------------------------------
More information低 剂 量 辐 射 对 脐 血 T 淋 巴 细 胞 膜 分 子 表 达 的 影 响 EFFECT OF LOW DOSE IRRADIATION ON EXPRESSION OF MEMBRANE MOLECULES OF T LYMPHOCYTES IN CORD BLOOD
CNIC-01513 PUTH-0001 低 剂 量 辐 射 对 脐 血 T 淋 巴 细 胞 膜 分 子 表 达 的 影 响 EFFECT OF LOW DOSE IRRADIATION ON EXPRESSION OF MEMBRANE MOLECULES OF T LYMPHOCYTES IN CORD BLOOD 中 国 核 情 报 中 心 China Nuclear Information
More informationPreparation of cord blood for infusion: bedside thaw, dilute and wash, or somewhere in between
Preparation of cord blood for infusion: bedside thaw, dilute and wash, or somewhere in between Donna Wall, MD Director, Manitoba Blood and Marrow Transplant Program ISCT 2012 Disclosures: none The problem:
More informationTransfection reagent for visualizing lipofection with DNA. For ordering information, MSDS, publications and application notes see www.biontex.
METAFECTENE FluoR Transfection reagent for visualizing lipofection with DNA For ordering information, MSDS, publications and application notes see www.biontex.com Description Cat. No. Size METAFECTENE
More informationCell Therapeutics - Process Development and Manufacturing for Human Clinical Trials
Cell Therapeutics - Process Development and Manufacturing for Human Clinical Trials Derek Hei, Ph.D. Director, Waisman Biomanufacturing University of Wisconsin - Madison April 9, 2013 Challenges in the
More informationOne of the most important recent developments in cancer treatment has been the ability to harvest stem
BIOTECHNOLOGY A Patient-Friendly Approach to Human Cell Transplantation One of the most important recent developments in cancer treatment has been the ability to harvest stem cells from bone marrow or
More informationCORD BLOOD BANKING FAQ
CORD BLOOD BANKING FAQ Cord Blood & Stem Cells Q: What is umbilical cord blood (UCB)? A: Bone marrow, peripheral blood and UCB constitute the three primary sources of stem cells. Cord blood, which, until
More informationMesenchymal stem cell extraction from human umbilical cord tissue: processing to understand and minimise variability in cell yield
Loughborough University Institutional Repository Mesenchymal stem cell extraction from human umbilical cord tissue: processing to understand and minimise variability in cell yield This item was submitted
More informationPr Eliane Gluckman, MD, FRCP, Disclosure of Interest: Nothing to Disclose
Pr Eliane Gluckman, MD, FRCP, Hospital Saint Louis, University Paris- Diderot, France Should Haplo-identical transplantation be preferred to cord blood in patients without a matched donor? Disclosure of
More informationNEWS RELEASE. For Immediate Release. For more information, please contact:
NEWS RELEASE For Immediate Release For more information, please contact: Charlene Han Singapore Cord Blood Bank Tel: (65) 6394-5017 charlene.han.j.j@scbb.com.sg Singapore Cord Blood Bank records 21 st
More informationAddition of Adipose-Derived Stem Cells in Cord Blood Cultures Stimulates Their Pluripotent Differentiation
STEM CELLS Addition of Adipose-Derived Stem Cells in Cord Blood Cultures Stimulates Their Pluripotent Differentiation N. Tsagias, K. Kouzi-Koliakos, I. Koliakos, E. Kostidou, V. Karagiannis, A. Daniilidis,
More informationCHARACTERISTIC FEATURES OF STEM CELLS. CLONING TECHNOLOGIES
CHARACTERISTIC FEATURES OF STEM CELLS. CLONING TECHNOLOGIES 1 Science is discovering the unknown Stem cell field is still in its infancy Human embryonic stem cell research is a decade old, adult stem cell
More informationCell Migration, Chemotaxis and Invasion Assay Protocol
Cell Migration, Chemotaxis and Invasion Assay Protocol Hilary Sherman, Pilar Pardo and Todd Upton Corning Life Sciences 900 Chelmsford Street Lowell, MA 01851 Introduction Cell migration, the movement
More informationStem Cell Research: Adult or Somatic Stem Cells
Chiang 1 Stem Cell Research: Adult or Somatic Stem Cells Abstract Kelly Chiang Cluster 7 Dr. LeFebvre 07/26/10 Over the past few decades, stem cells have been a controversial topic in the scientific field.
More informationA Public Cord Blood Bank for South Africa? i
No. 42/2007 A Public Cord Blood Bank for South Africa? i By Dr Robert Crookes MBChB (Wits), Dip. Internal Medicine (American Board of Internal Medicine, USA) Transfusion Medicine Consultant. South African
More informationStem Cells and Inner Ear Cell Regeneration. Stefan Heller Stanford University School of Medicine
Stem Cells and Inner Ear Cell Regeneration Stefan Heller Stanford University School of Medicine Embryonic stem cells Induced pluripotent stem cells Somatic stem cells 50 µm Derived from the inner cell
More informationProstaglandin E2 Enhances Human Cord Blood Stem. Cell Xenotransplants and Shows Long-Term Safety. in Preclinical Nonhuman Primate Transplant Models
Cell Stem Cell, Volume 8 Supplemental Information Prostaglandin E2 Enhances Human Cord Blood Stem Cell Xenotransplants and Shows Long-Term Safety in Preclinical Nonhuman Primate Transplant Models Wolfram
More informationBiological Stability and Activity of sirna in Ionic Liquids**
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Supporting Information Biological Stability and Activity of sirna in Ionic Liquids** Romiza R.
More informationCIGNA HEALTHCARE COVERAGE POSITION
CIGNA HEALTHCARE COVERAGE POSITION Subject Umbilical Cord Blood Banking Table of Contents Coverage Position... 1 General Background... 1 Coding/Billing Information... 3 References... 3 Revised Date...
More informationAlexander Smolyaninov,
«German-Russian Forum Biotechnology and Life Sciences» September, 2006 St. Petersburg Center of Cell and Gene Therapy «CRYOCENTER SAINT PETERSBURG» Stem Cell Bank «POKROVSKI» Alexander Smolyaninov, M.D.,Ph.D.,
More informationAutologous Cellular Therapies. Adrian Gee Center for Cell & Gene Therapy Baylor College of Medicine Production Assistance for Cellular Therapy (PACT)
Autologous Cellular Therapies Adrian Gee Center for Cell & Gene Therapy Baylor College of Medicine Production Assistance for Cellular Therapy (PACT) Autologous Cellular Therapies Definition Advantages
More informationAutomated Cord Blood Processing:
TM Automated Cord Blood Processing: A Follow-up to the Pilar Solves Study Comparing the AXP vs the Sepax Cord Blood Processing Systems CHRISTY KIM, PETRA CRAVENS, MINDY WILKE-DOUGLAS AND MITCHEL SIVILOTTI
More informationFactors Influencing Propagation Of Cultured Cord Blood-Derived Mesenchymal Stem Cells
Factors Influencing Propagation Of Cultured Cord Blood-Derived Mesenchymal Stem Cells Thesis Submitted for Fulfillment of the Master Degree in Clinical & Chemical Pathology By Dina Samir Abd El-Hady M.B.B.CH
More informationHuman Hepatic Sinusoidal Endothelial Cell Manual
Human Hepatic Sinusoidal Endothelial Cell Manual INSTRUCTION MANUAL SHIPPING CONDITIONS ZBM0093.00 Human Hepatic Sinusoidal Cells. Orders are delivered via Federal Express courier. All US and Canada orders
More informationThe Infinite Potential of Stem Cell Japan s Cord Blood Bank and Transplant
The Infinite Potential of Stem Cell Japan s Cord Blood Bank and Transplant Speech by Dr. Tsuneo A. Takahashi Translated by Stella Wang Japan and the United States are the two most experienced countries
More informationCell Cycle Phase Determination Kit
Cell Cycle Phase Determination Kit Item No. 10009349 Customer Service 800.364.9897 * Technical Support 888.526.5351 www.caymanchem.com TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 3 Safety
More informationRadius 24-Well Cell Migration Assay (Laminin Coated)
Product Manual Radius 24-Well Cell Migration Assay (Laminin Coated) Catalog Number CBA-125-LN 24 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Cell migration is a highly
More information