REFERENS RANGES. MEDICAL LABORATORY RAMUS Ltd. HAEMATOLOGY Name of the test Method Reference ranges Hemoglobin Cian-methaemoglobin method

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1 MEDICAL LABORATORY RAMUS Ltd REFERENS RANGES HAEMATOLOGY Name of the test Method Reference ranges Hemoglobin Cian-methaemoglobin method male g/l female g/l RBC Conductometric method male T/l female T/l WBC Conductometric method G/l MCV Conductometric method fl MCH Calculated pg MCHC Calculated g/l HCT Indirect determination on base conductometric male L/L female L/L method RDW Calculated PLT Conductometric after G/L flotation MPV Conductometric after fl flotation PCT Calculated L/L PDW Conductometric after flotation ERS Westergreen < 25 mm/h Differential count- automatic Name method Neo% MAPSS 44-76% Eo% MAPSS % Mo% MAPSS % Ba% MAPSS % Ly% MAPSS % Neo-count MAPSS G/l Eo-count MAPSS G/l Mo-count MAPSS 0,20-1,50 G/l Ba-count MAPSS 0,00-0,20G/l

2 2 Ly-count MAPSS G/l RDW Conductometric method MPV Conductometric method Differential count manual Name Method Normal St visual-optical < 0.06 Sg visual-optical 0, Eo visual-optical < 0.04 Mo visual-optical < 0.10 Ba visual-optical < 0.01 Ly visual-optical Reticulocytes visual-optical G/l COAGULATION Prothrombin time INR Chronometric method % aptt Chronometric method sec. TT Chronometric method 8-14 sec. Fibrinogen Chronometric method g/l Bleeding time Chronometric method s. Clothing time Chronometric method s. D dimmer MEIA < 600 ng/ml CLINICAL CHEMISTRY Glucose GOD/PAP mmol/l Total protein Biuret g/l Albumin Bromcresol green g/l Creatinine Jaffe mod µmol/l Urea Enzymatic Urease/GLDH mmol/l Uric acid Enzymatic color. Uricase- PAP male µmol/l female µmol/l Cholesterol Enzymatic color.chod- 6.5 mmol/l PAP HDL-direct Direct method mmol/l

3 3 cholesterol LDL-cholesterol Direct method 4.00 mmol/l VLDLcholesterol Calculated 0.90 mmol/l Triglycerides Enzymatic colorymetric- 2.2 mmol/l GPO-PAP Microalbumin Immunoturbudimetry Fresh urine 20 mg/dl 24-h urine 30 mg/l Glicated MEIA % hemoglobine Total bilirubin Jendrassik-Grof 21.0 µmol/l Direct bilirubin Jendrassik-Grof 8.0 µmol/l ASAT(GOT) IFCC 44.0 U/l ALAT(GPT) IFCC 44.0 U/l GGT Szasz male U/l female U/l ALP DGKC U/l CPK IFCC/SCE male U/l female U/l CK-MB Immuno UV H.P.-kinetic < 24.0 U/l LDH SFBC U/l Amylase IFCC Liquid EPS-PNP <100.0 U/l за серум <480.0 U/l за урина Potassium Direct potenciometry ISE mmol/l Sodium Direct potenciometry ISE mmol/l Chloride Direct potenciometry ISE mmol/l Calcium orto-cresolphtalein mmol/l complexon Ionized calcium Calculated mmol/l Inorganic Molybdate UV mmol/l phosphorus Magnesium Xylidyl blow mmol/l Iron Colorimetric-Ferrene male µmol/l female µmol/l TIBC Determination after saturation of the male µmol/l female µmol/l transferring by an iron solution of execess iron on magnesium hydroxide carbonate TSAT Calculated after TIBC and iron determination male % female % Lipase Colorimetric-kinetic 30U/l

4 4 Cholinesterase Colorimetric-kinetic Serum U/l Plasma U/l Lp(a) Immunoturbidimetry 30 mg/dl Apo B Immunoturbidimetry male 120 mg/dl female 90 mg/dl Apo AI Immunoturbidimetry male>110 mg/dl female>128 mg/dl HCO3 Colorimetric-kinetic mmol/l VIROLOGY HBsAg ELISA/MEIA A sample<cut off neg(-) Anti-HBs ELISA/MEIA A sample<cut off neg(-) anti-hav-igm ELISA/MEIA A sample<cut off neg(-) Anti-HCV ELISA/MEIA A sample<cut off neg(-) HBc-IgM ELISA/MEIA A sample<cut off neg(-) HIV ½ ELISA/MEIA A sample<cut off neg(-) EBV IgM ELISA < 0,9 neg (-); 0,9-1,0intermediate > 1,0 positive(+) EBV IgG ELISA < 16 neg (-); intermediate > 22 positive(+) Rubeolla IgM ELISA/ECLIA A sample<cut off neg(-) Rubeolla IgG ELISA/ECLIA < 8 neg (-); 8-11 intermediate > 11 positive(+) Лаймска болест IgM Лаймска болест IgG ELISA < 0,8 neg (-); 0,8-1,1 intermediate > 1,1 positive(+) ELISA < 16 neg (-); intermediate > 22 positive(+)

5 5 IMMUNOLOGY IgA Immunoturbudimerty g/l IgM Immunoturbudimerty male: g/l female: g/l IgG Immunoturbudimerty g/l IgE MEIA IU/ml C3 Immunoturbudimerty g/l С4 Immunoturbudimerty g/l CRP Immunoturbudimerty < 0,5 mg/dl US CRP Immunoturbudimerty < 5 mg/l CD 4+ Tcells (%) flow cytometry % CD 4+ Tcells flow cytometry /μl (count) Waler Rose Latex aglutination 8.0 IU/ml ASO Latex aglutination IU/ml ANA-detect ELISA < 1.0 neg (-); intermediate > 1,2 positive(+) Chlamydia Trahomatis IgM ELISA A sample<cut off neg(-) Chlamydia ELISA <1:8 neg(-) TrahomatisIgA Chlamydia ELISA <1:16 neg(-) TrahomatisIgG Helicobacter pylori IgG ELISA < 20 U/l neg (-); Apl IgG ELISA < 10 UI/ml Apl IgM ELISA < 10 UI/ml HORMONES TSH ECLIA/MEIA µiu/ml ft3 ECLIA/MEIA pg/ml ft4 ECLIA/MEIA ng/dl TAT ECLIA/MEIA < 115 IU/ml MAT ECLIA/MEIA < 34 IU/ml

6 6 TRAC ELISA <1,5 neg(-) TG ECLIA/MEIA 0,83-68,0 ng/ml Prolactine ECLIA/MEIA female ng/ml; male ng/ml Progesteron ECLIA/MEIA female: follicular phase ng/ml luteal phase ng/ml midcycle ng/ml pregnant: first trimester second trimester thirt trimester LH ECLIA/MEIA female: follicular phase miu/ml midcycle miu/ml luteal phase miu/ml postmenopausal miu/ml male: miu/ml FSH ECLIA/MEIA female:follicular phase midcycle miu/ml luteal phas miu/ml postmenopausal male: miu/ml Estradiol ECLIA/MEIA female: follicular phase pg/ml midcycle pg/ml luteal phase pg/ml postmenopausal 54.7 pg/ml male: pg/ml Testosterone ECLIA/MEIA female:midcycle ng/ml; male ng/ml β HCG MEIA < 5.0 miu/ml Cortizol MEIA μg/dl DHEA SO4 ECLIA Children up to 17 years < 280 ug/dl male, 407 µg/ml; female, 492 µg/ml Insulin MEIA uui/ml ACTH ECLIA ng/ml 17OHпрогесте рон ELISA female: follicular phase luteal phase , postmenopausal , male , children 0,2-0,9 PTH ECLIA ng/ml Osteocalcin ECLIA ng/ml Calcitonin ECLIA female: pg/ml male: pg/ml SHBG ECLIA female: male: Androstendion ECLIA female: ng/ml male: ng/ml

7 7 TUMOR MARKERS Total PSA ECLIA < 4.5 ng/ml Free PSA ECLIA <0.9 ng/ml CEA EIA <5.2 ng/ml CA 125 ECLIA/MEIA <35.0 U/ml CA 15-3 ECLIA/MEIA <25.0 U/ml CA 19-9 ECLIA/MEIA <37.0 U/ml AFP ECLIA <10.9 ng/ml SCC MEIA <1.5 ng/ml β2-microglobuline MEIA <2164 ng/ml NSE ЕIA <16,3 ng/ml Cyfra 21-1 ЕIA <3,3 ng/ml CA 72-4 ЕIA < 6.9 U/ml S-100 ЕIA <0.105 ng/ml HE 4 ЕIA < 150 METABOLYTES Folic acid MEIA/ECLIA ng/ml Vitamine B12 MEIA/ECLIA pg/ml Ferritin MEIA/ECLIA Female (18-30years): ng/ml; Postmenopausal: ng/ml Male(18-30years): ng/ml Male (31-60years) ng/ml Insulin MEIA µU/l Homocysteine FPIA/ECLIA µmol/l BNP MEIA < 100 pg/ml C- peptide FPIA/ECLIA ng/ml Troponin I MEIA 0.4 ng/ml (cut off AMI) 25-OH Vitamine D3 EIA > 20 ng/ml betacrosslaps EIA Female: Premenopausal: ng/ml;

8 8 Postmenopausal: ng/ml URINE ANALYSE ph Specific gravity Bilirubin Strip test (-) neg. Protein Strip test (-) neg. Glucose Strip test (-) neg. Urobilinogen Strip test N Ketones Strip test (-) neg. Nitrites Strip test (-) neg. Blood Strip test (-) neg. Leucocytes Strip test (-) neg. Sedimen Per field of vision Er up to 1 Leu up to 4 Epitelia up to 15 Bacteria-neg. OTHER TESTS Saliva Alcohol test Alcoholoxsydaze/Peroxydaze (-) neg. colorimetric method Carbamazepine ECLIA 4-12 μgr/ml Valproic acid ECLIA μgr/ml Phenobarbital ECLIA μgr/ml toxic >40 μgr/ml Valid for 2011 Clinical laboratory director /D-r Rossen Mihailov/