Sleva 25% při nákupu celého balení (násobku 12ks) následujících produktů Lonza Akce platí od

Size: px
Start display at page:

Download "Sleva 25% při nákupu celého balení (násobku 12ks) následujících produktů Lonza Akce platí od 9.2.2015-31.3.2015"

Transcription

1 Sleva 25% při nákupu celého balení (násobku 12ks) následujících produktů Lonza Akce platí od East Port Praha s.r.o., Možného 10, tel.: Kat. číslo Popis zboží Skladovací teplota HL-1 supplement 10 ml 2-8 C PC-1 Liquid 2 X 500 ml 2-8 C FBM-CD, 500 ml 2-8 C FGM-CD Single Quots Kit -20 C FGM-CD Bulletkit ( , ) mixed t, check components Q Hanks' BSS, w/o Ca++,Mg++ or Phenol Red, 1 L C Q Schneider's Drosophila Medium, Modified, 1 L 2-8 C F Grace's Insect Medium w/o FBS, Gent. Lah, 500 ml 2-8 C Q DPBS-1X w/ Ca++ & Mg++ Glucose and Pyr, 500 ml C F Grace's Insect Medium w/o FBS, 500 ml 2-8 C Q ProCHO 4 serum free media 1 L 2-8 C F Pen/Strep stock, 20K/20K 500 ml -20 C E RPMI 1640 w/ HEPES, Pen- Strep, and L-Gln, 100 ml 2-8 C F RPMI 1640 w/ HEPES, Pen- Strep, and L-Gln, 500 ml 2-8 C Q Hanks' BSS, with Phenol Red, 1 L C B Dextrose Gelatin Veronal Buffer, 500 ml C Q Hanks' BSS, w/o Ca++,Mg++ w/ phenol red, 1 L C E ACK Lysing Buffer 100 ml C Q RPMI 1640 with HEPES and L-glutamine, 1 L C Q Medium199 w/ebss, L-Gln, HEPES 2.2g/L NaHCO3 1 L 2-8 C Q MEM Eagle EBSS w/o L-Gln 1 L C A Freezing Medium 100 ml C F MEM Eagle HBSS w/o L-Gln w/hepes, 500 ml C F/24 RPMI 1640 without L-glutamine, 24 x 500 ml C Q RPMI 1640 w/o L-Glutamine 1 L C Q MEM Eagle with Earle's BSS, with L-glutamine, 1 L 2-8 C Q DMEM 4.5 g/l glucose without L-glutamine, 1 L 2-8 C E Veronal Buffer (5X) 100 ml C Q MEM Eagle EBSS w/ NEAA, Pyr, w/o L-Gln, 1 L 2-8 C E MEM Eagle EBSS (2x), w/o L-Gln, Phenol Red, 100 ml C E L-15 (2x) w/o L-Glutamine or Phenol Red, 100 ml 2-8 C Q L-15 (Leibovitz), without L-Gln, 1 L 2-8 C Q RPMI 1640 with L-glutamine, 1 L C Q DMEM:F12 1:1Mixture with HEPES, L-Gln, 1 L 2-8 C Q IMDM with HEPES and L-Gln, 1 L 2-8 C B UltraDOMA serum-free hybridoma medium 500 ml 2-8 C Q UltraCHO w/l-glutamine 1 L 2-8 C Q IMDM with HEPES w/o L-Gln, 1 L 2-8 C F UltraDOMA-PF Hybridoma Medium, 500 ml 2-8 C Q DMEM 4.5 g/l Glucose w/o L-Gln or Pyr, 1 L 2-8 C

2 12-736E MEM Eagle EBSS, Maint. 100 ml 2-8 C F MEM Eagle EBSS, Maint. 500 ml 2-8 C F UltraSaline A without Phenol Red, 500 ml 2-8 C Q Pro293S-CDM, for suspension cells, 1 L 2-8 C E ProFreeze NAO/CD Freeze Media Medium, 2X, 100 ml 2-8 C Q PowerCHO-1 serum-free medium 1L 2-8 C Q ProNS0-1, 1 L 2-8 C Q ProNS0-2, 1 L 2-8 C J ProNS0 Lipid CD Supplement, with cholesterol -20 C E NCTC-109 w/ EBSS, & L-Gln hybridoma tested, 100 ml 2-8 C C MEM Eagle Vitamin mix (100x), 50 ml -20 C L Gentamicin 50 mg/ml 10 x 10 ml screw cap vial C Z Gentamicin 50 mg/ml 1 x 10 ml screw cap vial C L Gentamicin 10 mg/ml 10 x 10 ml screw cap vial C Z Gentamicin 10 mg/ml 1 x 10 ml screw cap vial C Z Gentamicin 50 mg/ml 1 x 10 ml crimp-top vial C C L-Glutamine 200mM 50ml -20 C R Pen/Strep/L-Gln mixture 25K/25K/200mM, 25x4.5 ml -20 C F HEPES Buffer, 1M stock in normal saline, 500 ml C E Pen/Strep/Fungizone 10K/10K/25ug, 100 ml -20 C H Pen/Strep/Fungizone 10K/10K/25ug, 20 ml -20 C E Lymphocyte Separ. Med. 100 ml C E/24 Lymphocyte Separation Medium 1.077, 24 x 100 ml C F Lymphocyte Separation Medium 500 ml C R Fungizone(Amphotericin B) 250 ug/ml, 20 ml -20 C Z ITS (500x stock) 5 ml -20 C Z ITES (500x stock) 5 ml -20 C C L-Glutamine 200mM Hybricoma tested, 50 ml -20 C E Trypan Blue 0.4% solution 100 ml C BE02-002F MEM Alpha 500 ml 2-8 C BE02-007E Trypsin/EDTA 10X 100 ml -20 C BE02-011F TC 100 with L-Gln 500 ml 2-8 C BE02-012E Gentamicin Sulfate 100 ml C BE02-014F Ham's F10 Medium 2 U-Gln 1, 500 ml 2-8 C BE02-015BOX Lymphochrome 10 x 5 ml -20 C BE02-015E Lymphochrome 100 ml -20 C BE02-015F Lymphochrome 500 ml -20 C BE02-016Q ProCHO AT 1 L 2-8 C BE02-017F PBS EDTA 500 ml C BE02-019F William's Medium E wo L-gln wo Phenol Red, 500 ml 2-8 C BE02-025Q Pro293S-CDM, for suspension cells, 1 L 2-8 C BE02-026E Amniochrome Plus 100 ml -20 C BE02-026F Amniochrome Plus 500 ml -20 C BE02-027F EBSS with HEPES 500 ml C BE02-028Q ProPer 1 Medium 1 L 2-8 C BE02-029Q ProDoma 1 1 L 2-8 C BE02-030Q ProVero 1 Medium 1 L 2-8 C BE02-032Q ProDoma 3 1 L 2-8 C BE02-034E Trypzean /EDTA 10X 100 ml -20 C BE02-035E Amniochrome Pro 100 ml -20 C

3 BE02-035F Amniochrome Pro 500 ml -20 C BE02-040E Glucose 100 g/l C BE02-041Q ProCHO-5 w/o glucose 2-8 C BE02-042Q PowerCHO-2 w/o glucose 2-8 C BE02-053Q X-Vivo15(04-418) contains rec transf, 1L 2-8 C BE02-054Q X-Vivo15(04-744) contains rec transf, 1L 2-8 C BE02-055Q X-VIVO10(04-743) contains rec transf -1L 2-8 C BE04-380Q X-VIVO 10 with Gentamicin and Phenol Red, 1 L 2-8 C BE04-418F X-VIVO 15 with Gentamicin and Phenol Red, 500 ml 2-8 C BE04-418Q X-VIVO 15 with Gentamicin and Phenol Red, 1 L 2-8 C BE04-448Q X-VIVO 20 with Gentamicin and Phenol Red, 1 L 2-8 C BE04-558F RPMI 1640 with HEPES wo L-Gln, 500 ml C BE04-684E UltraGlutamine II Supplement 200 mm L-glycyl-L-glutamine, 100 ml C BE04-687F/U1 DMEM:F12 1:1 wo HEPES w U-Gln 1, 500 ml 2-8 C BE04-687Q DMEM:F12 1:1 wo HEPES 1 L 2-8 C BE04-743Q X-VIVO 10 w/o Gentamicin or Phenol Red, 1 L 2-8 C BE04-744Q X-VIVO 15 w/o Gentamicin or Phenol Red, 1 L 2-8 C BE06-174G MEM Eagle-EBSS w/ NEAA, 450 ml 2-8 C BE10-502F Earle's BSS 500 ml C BE10-508F Hanks' BSS, with Phenol Red, 500 ml C BE10-527F Hanks' BSS, without Phenol Red, 500 ml C BE10-543F HBSS w/ PR w/o Ca, Mg 500ml C BE10-547F Hanks' BSS, w/o Ca++,Mg++ or Phenol Red, 500 ml C BE12-029Q ProCHO 4 - CDM 1 L 2-8 C BE12-105F BME with Earle's BSS 500 ml C BE12-109F Medium199 w/hbss, L-Gln, 1.4g/L NaHCO3, 500 ml 2-8 C BE12-115F RPMI 1640 w/ 25mM HEPES 500ml 2-8 C BE12-115F/U1 RPMI 1640 with UltraGlutamine I and HEPES, 500 ml C BE12-117F Med 199 w/ebss, L-Gln w/25 mm Hepe 500ml 2-8 C BE12-118F Medium199 w/hbss, L-Gln, HEPES 1.4g/L NaHCO3 500ml 2-8 C BE12-119F Medium199 w/ebss, L-Gln, 2.2g/L NaHCO3, 500 ml 2-8 C BE12-125F MEM Eagle with EBSS w/o L-Gln, 500 ml C BE12-127F MEM Eagle HBSS w/o L-Gln 500 ml C BE12-136F MEM-Eagle-EBSS w/hepes w/o L-Glut 500 ml C BE12-136Q MEM Eagle EBSS w/o L-Gln w/hepes, 1 L C BE12-167F RPMI 1640 w/o L-Glutamine 500ml C BE12-168F McCoy's 5A w/ L-Gln, w/hepes, 500 ml 2-8 C BE12-169F Alpha MEM Eagle w/o L-Glutamine 500ml 2-8 C BE12-604F DMEM 4.5g/L Glucose w/ L-Glutamine 500ml 2-8 C BE12-604F/U1 DMEM with UltraGlutamine I, 500 ml 2-8 C BE12-604Q DMEM 4.5 g/l Glucose w/ L-Gln, 1L 2-8 C BE12-604Q DMEM 4.5 g/l Glucose w/ L-Gln, 1L 2-8 C BE12-611F MEM Eagle-EBSS 500ml 2-8 C BE12-614F DMEM w/ 4.5g/L glucose w/o L-Glut 500ml 2-8 C BE12-615F Ham's F-12 w/ L-Glutamine 500ml 2-8 C BE12-618F IMDM with HEPES, without L-glutamine, 500 ml 2-8 C BE12-662F MEM Eagle-EBSS w/ NEAA w/o L-Gln 500ml 2-8 C BE12-668F EMEM (2x) w/o L-Glu, PR L-Gln, Phenol Red, 500 ml C BE12-684F MEM Eagle EBSS (10X) w/o NaHCO3, L-Gln, 500 ml C BE12-688F McCoy's 5A w/ L-Gln 500 ml 2-8 C

4 BE12-700F L-15 (Leibovitz), without L-Glutamine, 500 ml 2-8 C BE12-702F RPMI 1640 w/ L-Glutamine 500ml 2-8 C BE12-702F/U1 RPMI 1640 with UltraGlutamine I, 500 ml C BE12-707F DMEM w/1,0 g/l glucose w/o L-Glut, 500ml 2-8 C BE12-708F DMEM 1.0 g/l Glucose w/o L-Gln, w/hepes 500 ml 2-8 C BE12-709F DMEM 4,5g/L Glu,25mM HEPE,w/oL-Gln 500ml 2-8 C BE12-719F DMEM:F-12 1:1 w/15mm Hepes, L-Gln 500ml 2-8 C BE12-722F IMDM w/ 25 mm HEPES 500ml 2-8 C BE12-723F Ultradoma Medium 500 ml 2-8 C BE12-725F UltraCULTURE Serum-free Medium, w/o L-Glut,500 ml 2-8 C BE12-726F IMDM with HEPES w/o L-Glutamine, 500 ml 2-8 C BE12-730F Insect-XPRESS w/ L-Gln 500 ml 2-8 C BE12-730Q Insect-XPRESS w/ L-Gln 1 L 2-8 C BE12-733F DMEM w/4.5g/l gluc w/o L-Gln, Na Pyruvat 2-8 C BE12-739F MEM, Glasgow's (G-MEM) w/o glucose, 500 ml 2-8 C BE12-741F DMEM high glucose w/o Na Pyruvate 500ml 2-8 C BE12-743F UltraMEM with ITES with L-Glutamine, 500 ml 2-8 C BE12-749Q UltraMDCK Serum-free Medium, 1 L 2-8 C BE12-752F RPMI 1640 with L-Gln, w/o glucose, 500 ml 2-8 C BE12-756EZM Amniochrome II 100 ml 2-8 C BE12-756FCM Amniochrome II 500 ml 2-8 C BE12-761F William's Medium E, 500 ml 2-8 C BE12-764Q Pro293A-CDM, for adherent cells, 1 L 2-8 C BE12-766Q ProCHO 5 - CDM 1 L 2-8 C BE12-771Q PowerCHO-2 serum-free media 1L 2-8 C BE12-772Q PowerCHO-3 serum-free media 1L 2-8 C BE12-776Q PowerCHO - GS, 1L bottle 2-8 C BE12-914F DMEM 4.5 g/l Glucose w/o L-Gln HybridomaTest 500ml 2-8 C BE12-915F IMDM with HEPES, L-Gln Hybridoma tested, 500 ml 2-8 C BE12-917F DMEM 4.5 g/l Glucose w/o L-Gln,Phenol Red, 500 ml 2-8 C BE12-918F RPMI 1640 without L-Gln or Phenol Red, 500 ml C BE13-114E Non-essential amino acids 10 mm 100x stock, 100 ml 2-8 C BE13-115E Sodium Pyruvate Solution 100 mm, 100 ml 2-8 C BE17-160E Trypsin(10x) 2.5% in HBSS w/o Ca, Mg, 100 ml -20 C BE17-161E Trypsin-Versene (EDTA) Mix (1X) 100ml -20 C BE17-161F Trypsin-Versene (EDTA) 500 ml -20 C BE17-512F DPBS M(PO4) w/o Ca and Mg, 500ml C BE17-512Q DPBS-1X w/o Ca++, Mg++ 1 L C BE17-513F DPBS-1X w/ Ca, Mg 500ml C BE17-513Q DPBS-1X w/ Ca++ & Mg++ 1 L C BE17-515F DPBS-10X w/o Ca++, Mg ml C BE17-515Q DPBS-10X w/o Ca++, Mg++ 1 L C BE17-516F PBS-1X w/o Ca, Mg 500ml C BE17-516Q PBS-1X, w/o Ca++, Mg++ 1 L C BE17-517Q PBS-10X, w/o Ca++, Mg++ 1 L C BE17-524CM AMNIOCHROME II supplement modified 35 ml -20 C BE17-605E L-Glutamine 200mM 100ml -20 C BE17-605E/U1 UltraGlutamine I Supplement 200 mm (100X) Alanyl-L-glutamine, 100 ml C BE17-605F L-Glutamine 200mM 500 ml -20 C BE17-613E Sodium Bicarb. 7.5% sol 100 ml C

5 BE17-711E Versene (EDTA), 0.02% 100 ml C BE17-724F Water for Cell Culture 500 ml C BE17-724Q Water for Cell Culture 1 L C BE17-737E HEPES Buffer, 1M stock in normal saline, 100 ml C BE17-855E ProHT Supplement (100x) 100 ml -20 C BE77201 HL-1 Chemically Defined, Serum-free Medium for hybridoma mixed and hematopoietic t, check components cells, without L-glutam DE17-602E Pen/Strep stock, 10K/10K 100 ml -20 C DE17-602F Pen/Strep stock, 10K/10K 500 ml -20 C DE17-603E Pen/Strep stock, 5K/5K 100 ml -20 C

Akce Lonza na média pro primární tkáňové kultury a buňky

Akce Lonza na média pro primární tkáňové kultury a buňky Akce Lonza na média pro primární tkáňové kultury a buňky Seznam produktů se slevou 25%. V případě nákupu balíčku primárních buněk Lonza a příslušného média je celková sleva 30%. Konkrétní nabídku Vám rádi

More information

Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin

Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin Human Tubal Fluid (HTF) Media & Modifi ed Human Tubal Fluid (mhtf) Medium with Gentamicin HTF Media are intended for use in assisted reproductive procedures which include gamete and embryo manipulation

More information

ATCC ANIMAL CELL CULTURE GUIDE

ATCC ANIMAL CELL CULTURE GUIDE ATCC ANIMAL CELL CULTURE GUIDE tips and techniques for continuous cell lines The Essentials of Life Science Research Globally Delivered Table of Contents This guide contains general technical information

More information

NCL Method ITA-6. Leukocyte Proliferation Assay

NCL Method ITA-6. Leukocyte Proliferation Assay NCL Method ITA-6 Leukocyte Proliferation Assay Nanotechnology Characterization Laboratory National Cancer Institute-Frederick SAIC-Frederick Frederick, MD 21702 (301) 846-6939 ncl@mail.nih.gov December

More information

ArC Amine Reactive Compensation Bead Kit

ArC Amine Reactive Compensation Bead Kit ArC Amine Reactive Compensation Bead Kit Catalog no. A1346 Table 1. Contents and storage information. Material Amount Composition Storage Stability ArC reactive beads (Component A) ArC negative beads (Component

More information

Modulating Glucose Uptake in Skeletal Myotubes:

Modulating Glucose Uptake in Skeletal Myotubes: icell Skeletal Myoblasts Application Protocol Introduction Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis The skeletal muscle is one of the

More information

Xpert TM Animal Tissue Culture Teaching Kit

Xpert TM Animal Tissue Culture Teaching Kit Xpert TM Animal Tissue Culture Teaching Kit Product Code: CCK005 Contents 1. About the kit 2. Kit contents and storage instructions 3. Materials required but not provided in the kit 4. Aseptic techniques

More information

NCL Method ITA-14. Analysis of Nanoparticle Effects on Maturation of Monocyte Derived Dendritic Cells In Vitro

NCL Method ITA-14. Analysis of Nanoparticle Effects on Maturation of Monocyte Derived Dendritic Cells In Vitro NCL Method ITA-14 Analysis of Nanoparticle Effects on Maturation of Monocyte Derived Dendritic Cells In Vitro Nanotechnology Characterization Laboratory Frederick National Laboratory for Cancer Research

More information

How To Safely Use An Ivm System

How To Safely Use An Ivm System Material Safety Data Sheet Document no: MSDS-32 Product: MediCult IVM System Page 1 /3 Date first created: 06/12/18 Version 2 Date revised: 09/12/04 1. Identification of Substance/Preparation and of the

More information

Catalog 2013 2014 Advancing Research with Biologically Relevant Solutions

Catalog 2013 2014 Advancing Research with Biologically Relevant Solutions BioResearch Catalog 2013 2014 Advancing Research with Biologically Relevant Solutions Primary Cells and Media Media, Reagents and Sera Mycoplasma Detection and Prevention Transfection BioAssay Products

More information

Cell Phone Radiation Effects on Cancer. Tyler Barkich Central Catholic High School Grade 11

Cell Phone Radiation Effects on Cancer. Tyler Barkich Central Catholic High School Grade 11 Cell Phone Radiation Effects on Cancer Tyler Barkich Central Catholic High School Grade 11 Cancer Overview Cancer cells are cells that grow and divide at an irregular, unregulated pace. Apoptosis does

More information

Amaxa 4D-Nucleofector Protocol for Mouse Embryonic Stem [ES] Cells For 4D-Nucleofector X Unit Transfection in suspension

Amaxa 4D-Nucleofector Protocol for Mouse Embryonic Stem [ES] Cells For 4D-Nucleofector X Unit Transfection in suspension For 4D-Nucleofector X Unit Transfection in suspension Cells derived from mouse blastocysts; round cells, growing in clumps s 1. This protocol is meant to provide an outline for the handling and the Nucleofection

More information

human Melatonin Receptor 1 (MT 1 ) Cell Line

human Melatonin Receptor 1 (MT 1 ) Cell Line TECHNICAL DATA SHEET ValiScreen GPCR Cell Line Caution: For Laboratory Use. A research product for research purposes only human Melatonin Receptor 1 (MT 1 ) Cell Line Product No.: ES-620-C Lot No.: M1W-C1

More information

BUFFERS and MEDIAS Coomassie Blue Staining Solution Coomassie blue Destaining Solution DMEM Normal Cell Culture Media

BUFFERS and MEDIAS Coomassie Blue Staining Solution Coomassie blue Destaining Solution DMEM Normal Cell Culture Media BUFFERS and MEDIAS Coomassie Blue Staining Solution 2 g Coomassie Blue 2 L Methanol or Ethanol * 1.6 L 400 ml Glacial acetic acid *If you will be microwaving the gel in staining solution for rapid staining

More information

TABLE OF CONTENT. Page ACKNOWLEDGEMENTS. iii ENGLISH ABSTRACT THAI ABSTRACT. vii LIST OF TABLES LIST OF FIGURES. xvi ABBREVIATIONS.

TABLE OF CONTENT. Page ACKNOWLEDGEMENTS. iii ENGLISH ABSTRACT THAI ABSTRACT. vii LIST OF TABLES LIST OF FIGURES. xvi ABBREVIATIONS. x TABLE OF CONTENT ACKNOWLEDGEMENTS ENGLISH ABSTRACT THAI ABSTRACT LIST OF TABLES LIST OF FIGURES ABBREVIATIONS iii iv vii xv xvi xviii CHAPTER I: INTRODUCTION 1.1 Statement of problems 1 1.2 Literature

More information

Taqman TCID50 for AAV Vector Infectious Titer Determination

Taqman TCID50 for AAV Vector Infectious Titer Determination Page 1 of 8 Purpose: To determine the concentration of infectious particles in an AAV vector sample. This process involves serial dilution of the vector in a TCID50 format and endpoint determination through

More information

Standard Operating Procedures (SOPs) for the production and testing of embryonic stem cell lines in Taiwan Stem Cell Bank

Standard Operating Procedures (SOPs) for the production and testing of embryonic stem cell lines in Taiwan Stem Cell Bank Standard Operating Procedures (SOPs) for the production and testing of embryonic stem cell lines in Taiwan Stem Cell Bank Bioresource Collection and Research Centre (BCRC) at Food Industry Research and

More information

Validation & Assay Performance Summary

Validation & Assay Performance Summary Validation & Assay Performance Summary GeneBLAzer RAR alpha DA Assay Kit GeneBLAzer RAR alpha-uas-bla GripTite Cells Cat. no. K1387, K1685 Target Description The retinoic acid receptor alpha (RAR alpha)

More information

Poietics human mesenchymal stem cells Instructions for use

Poietics human mesenchymal stem cells Instructions for use Poietics human mesenchymal stem cells Instructions for use www.lonza.com U.S. Scientific Support: 800-521-0390 scientific.support@lonza.com EU/ROW Scientific Support: +49-221-99199-400 scientific.support.eu@lonza.com

More information

Chromatin Immunoprecipitation (ChIP)

Chromatin Immunoprecipitation (ChIP) Chromatin Immunoprecipitation (ChIP) Day 1 A) DNA shearing 1. Samples Dissect tissue (One Mouse OBs) of interest and transfer to an eppendorf containing 0.5 ml of dissecting media (on ice) or PBS but without

More information

Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual

Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.03 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+ Progenitor Cells, cryopreserved Cryopreserved human umbilical

More information

RayBio Creatine Kinase (CK) Activity Colorimetric Assay Kit

RayBio Creatine Kinase (CK) Activity Colorimetric Assay Kit RayBio Creatine Kinase (CK) Activity Colorimetric Assay Kit User Manual Version 1.0 May 28, 2014 RayBio Creatine Kinase Activity Colorimetric Assay (Cat#: 68CL-CK-S100) RayBiotech, Inc. We Provide You

More information

ab102884 FITC Conjugation Kit Protocol

ab102884 FITC Conjugation Kit Protocol ab102884 FITC Conjugation Kit Protocol Antibody and protein modification This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 4 November 2013 Table of Contents

More information

Creatine Kinase Activity Colorimetric Assay Kit ABE5487 100 assays; Store at -20 C

Creatine Kinase Activity Colorimetric Assay Kit ABE5487 100 assays; Store at -20 C Creatine Kinase Activity Colorimetric Assay Kit ABE5487 100 assays; Store at -20 C I. Introduction: Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N- phosphotransferase

More information

Technologies for Monoclonal Antibody Production

Technologies for Monoclonal Antibody Production Technologies for Monoclonal Antibody Production First we help you bridge the distance between upstream and downstream. Then we help you shorten it. Monoclonal antibodies for therapeutic, diagnostic and

More information

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Chromatin Immunoprecipitation A) Prepare a yeast culture (see the Galactose Induction Protocol for details). 1) Start a small culture (e.g. 2 ml) in YEPD or selective media from a single colony. 2) Spin

More information

Islet Viability Assessment by Single Cell Flow Cytometry

Islet Viability Assessment by Single Cell Flow Cytometry Islet Viability Assessment by Single Cell Flow Cytometry Page 1 of 8 Purpose: To comprehensively assess the viability of the islet cell preparation prior to transplantation. Tissue Samples: A sample containing

More information

Application Note USD3013. Monoclonal Antibody Production in Suspension CHO Culture Using Single-Use PadReactor Mini Bioreactor

Application Note USD3013. Monoclonal Antibody Production in Suspension CHO Culture Using Single-Use PadReactor Mini Bioreactor Application Note USD313 Monoclonal Antibody Production in Suspension CHO Culture Using Single-Use PadReactor Mini Bioreactor Introduction Single use solutions are found in many applications as they offer

More information

COUNTY OF SAN DIEGO EMERGENCY MEDICAL SERVICES No. P-117a POLICY/PROCEDURE/PROTOCOL Page 1 of 6

COUNTY OF SAN DIEGO EMERGENCY MEDICAL SERVICES No. P-117a POLICY/PROCEDURE/PROTOCOL Page 1 of 6 POLICY/PROCEDURE/PROTOCOL Page 1 of 6 GREY/PINK Kg range: < 8 kg Approx Kg: 5 kg 1 st 2 nd 3 rd Approximate LBS: 10 lbs Defib: 10 J 20 J 20 J ET uncuffed tube size: 3.5 Cardiovert: 5 J 10 J 10 J ET cuffed

More information

EVALUATION OF THE EFFECT OF THE COMPOUND RIBOXYL ON THE ATP LEVEL AND CELLULAR RESPIRATION FROM HUMAN DERMAL FIBROBLASTS

EVALUATION OF THE EFFECT OF THE COMPOUND RIBOXYL ON THE ATP LEVEL AND CELLULAR RESPIRATION FROM HUMAN DERMAL FIBROBLASTS EVALUATION OF THE EFFECT OF THE COMPOUND RIBOXYL ON THE ATP LEVEL AND CELLULAR RESPIRATION FROM HUMAN DERMAL FIBROBLASTS FINAL REPORT OF THE STUDY GT050923 Study promoter: LUCAS MEYER COSMETICS ZA Les

More information

Growth and Maintenance of T-REx Cell Lines

Growth and Maintenance of T-REx Cell Lines USER GUIDE Growth and Maintenance of T-REx Cell Lines Catalog Numbers R710-07, R714-07, R718-07, R722-07 Document Part Number 25-0272 Publication Number MAN0000106 Revision 3.0 For Research Use Only. Not

More information

Follicle Dermal Papilla Cell

Follicle Dermal Papilla Cell Follicle Dermal Papilla Cell Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product Description

More information

GASTRIC ORGANOID CULTURE PROTOCOL

GASTRIC ORGANOID CULTURE PROTOCOL GASTRIC ORGANOID CULTURE PROTOCOL THIS PROTOCOL PROVIDES THE PROCEDURE FOR SUBCULTURING NORMAL HUMAN GASTRIC ORGANOIDS WHICH WAS DERIVED FROM THE SUBMERGED METHOD AS DESCRIBED IN BARKER N, ET AL. LGR5+VE

More information

123count ebeads Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use.

123count ebeads Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use. Page 1 of 1 Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use. Normal human peripheral blood was stained with Anti- Human CD45 PE (cat.

More information

I. ACID-BASE NEUTRALIZATION, TITRATION

I. ACID-BASE NEUTRALIZATION, TITRATION LABORATORY 3 I. ACID-BASE NEUTRALIZATION, TITRATION Acid-base neutralization is a process in which acid reacts with base to produce water and salt. The driving force of this reaction is formation of a

More information

AlphaLISA Human Insulin kit

AlphaLISA Human Insulin kit Technical Data Certificate of Analysis Caution: For Laboratory Use. Research chemicals for research purposes only. AlphaLISA Insulin Kit PRODUCT NUMBERS: AL204C (500 ASSAY POINTS) AL204F (5 000 ASSAY POINTS)

More information

Essential Maths for Medics and Vets Reference Materials Module 2. Amount and Concentration.

Essential Maths for Medics and Vets Reference Materials Module 2. Amount and Concentration. 2 Amount and concentration: making and diluting solutions 2 Amount and concentration; making and diluting solutions... 2A Rationale... 2B Distinguishing between amount and concentration, g and %w/v...

More information

Chapter 15. Reconstitution and Dosages Measured in Units. Copyright 2012, 2007 Mosby, Inc., an affiliate of Elsevier Inc. All rights reserved.

Chapter 15. Reconstitution and Dosages Measured in Units. Copyright 2012, 2007 Mosby, Inc., an affiliate of Elsevier Inc. All rights reserved. Chapter 15 Reconstitution and Dosages Measured in Units 1 Objectives Calculating drug dosage problems that first require reconstitution of a powdered drug into a liquid form Using a proportion to solve

More information

SSI STREPTOCOCCUS LATEX GROUP KIT

SSI STREPTOCOCCUS LATEX GROUP KIT SSI STREPTOCOCCUS LATEX GROUP KIT SSI STREPTOCOCCUS LATEX GROUP KIT Latex particles coated with streptococcal antiserum raised in rabbits Application The Streptococcus Latex Group Kit is a ready-to-use

More information

DRUG CALCULATIONS FOR REGISTERED NURSES ADULT SERVICES

DRUG CALCULATIONS FOR REGISTERED NURSES ADULT SERVICES Serving the people of north east Essex DRUG CALCULATIONS FOR REGISTERED NURSES ADULT SERVICES A Programmed Approach - It is essential you read this pack & practice the questions prior to your drug calculation

More information

Divisione Biologia Cellulare

Divisione Biologia Cellulare Divisione Biologia Cellulare Informazioni Centralino Tel. Fax. e-mail 02 93 991 90 02 93 991 001 info@mmedical.dgroup.it Gestione Gare ed Offerte Fax. 02 93 991 001 Triveneto Emilia Romagna Marche Umbria

More information

Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856

Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856 Nickel DOC316.53.01065 Dimethylglyoxime Method Method 10220 0.1 to 6.0 mg/l Ni TNTplus 856 Scope and application: For water and wastewater. Test preparation Instrument-specific information Table 1 shows

More information

Mesenchymal Stem Cells

Mesenchymal Stem Cells Mesenchymal Stem Cells Instruction Manual Product Size Catalog Number from Bone Marrow (hmsc-bm) from Umbilical Cord Matrix (hmsc-uc) from Adipose Tissue (hmsc-at) 500,000 cryopreserved cells 500,000 proliferating

More information

RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method)

RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Immune Tolerance Network RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Author: Paul Wallace, Director, RPCI Laboratory of Flow Cytometry Approved by: Paul

More information

SERUM FREE MEDIA FOR CELL CULTURE

SERUM FREE MEDIA FOR CELL CULTURE SERUM FREE MEDIA FOR CELL CULTURE A Dr Hadwen Trust/FRAME initiative on behalf of Focus on Alternatives. This document provides an overview of the range of commercially available serum free media for cell

More information

Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-806)

Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-806) Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-86) The Hoechst purification was established for murine hematopoietic stem cells (HSC)

More information

Cell Migration, Chemotaxis and Invasion Assay Protocol

Cell Migration, Chemotaxis and Invasion Assay Protocol Cell Migration, Chemotaxis and Invasion Assay Protocol Hilary Sherman, Pilar Pardo and Todd Upton Corning Life Sciences 900 Chelmsford Street Lowell, MA 01851 Introduction Cell migration, the movement

More information

Lleve más, por menos.

Lleve más, por menos. Lleve más, por menos. Código promo: AR1220 Código Detalle Presentación CULTIVO CELULAR 11360070 0010057AE A1327504 A1148301 12681011 11965092 10566016 11995040 11995065 10313021 12100046 12800017 12800082

More information

Basic Science in Medicine

Basic Science in Medicine Medical Journal of th e Islamic Republic of Iran Volume 18 Number 3 Fall 1383 November 2004 Basic Science in Medicine ] EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN

More information

HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs)

HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) OBJECTIVE: can be cryopreserved in a liquid nitrogen (LN 2 ) freezer for long-term storage. This Standard Operating Procedure (SOP)

More information

Reconstitution of Solutions

Reconstitution of Solutions Chapter 12 Reconstitution of Solutions Reconstitution Process of mixing and diluting solutions Some medications supplied in powder form and must be mixed with liquid before administration Parts of Solutions

More information

MEF Starter Nucleofector Kit

MEF Starter Nucleofector Kit page 1 of 7 MEF Starter Nucleofector Kit for Mouse Embryonic Fibroblasts (MEF) MEF display significant phenotypic variations which depend on the strain, the genetic background of the mice they are isolated

More information

PROTOCOL. Immunocytochemistry (ICC) MATERIALS AND EQUIPMENT REQUIRED

PROTOCOL. Immunocytochemistry (ICC) MATERIALS AND EQUIPMENT REQUIRED PROTOCOL Immunocytochemistry (ICC) 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 11-07 MATERIALS AND EQUIPMENT REQUIRED Materials: MitoSciences primary monoclonal antibody/antibodies Fluorophore-conjugated

More information

Protocols for Adipogenic Differentiation Assays for Characterization of Adipose Stromal Cells (ASC)

Protocols for Adipogenic Differentiation Assays for Characterization of Adipose Stromal Cells (ASC) Protocols for Adipogenic Differentiation Assays for Characterization of Adipose Stromal Cells (ASC) Purpose: To characterize the adipogenic differentiation capabilities of adipose stromal cells. Overview:

More information

LIVE/DEAD Fixable Dead Cell Stain Kits

LIVE/DEAD Fixable Dead Cell Stain Kits USER GUIDE LIVE/DEAD Fixable Dead Cell Stain Kits Pub. No. MAN0002416 (MP34955) Rev. A.0 Table 1. Contents and storage Material Amount Storage Stability Individual Kits: Blue, violet, aqua, yellow-, green,

More information

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605 LACTIC ACID Fasting, arterial specimen preferred. Please note whether arterial or venous. 0.5 ml heparinized plasma. Green top or PST must be drawn, placed on ice, and spun within 15 minutes. Immediately

More information

Lipid Extraction Kit (Chloroform Free)

Lipid Extraction Kit (Chloroform Free) Product Manual Lipid Extraction Kit (Chloroform Free) Catalog Number STA-612 50 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipids are a diverse group of molecules that

More information

GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS

GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS ST-PETERSBURG STATE UNIVERSITY THEODOSIUS DOBZHANSKY CENTER FOR GENOME BIOINFORMATICS GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS 2015 The object of

More information

Human Adult Stem Cell Manual Differentiation protocols for human adipose-derived adult stem cells

Human Adult Stem Cell Manual Differentiation protocols for human adipose-derived adult stem cells Human Adult Stem Cell Manual Differentiation protocols for human adipose-derived adult stem cells INSTRUCTION MANUAL ZBM0015.01 SHIPPING CONDITIONS Human Adult Stem Cells (ASC) Orders are delivered via

More information

PROTEINS (LOWRY) PROTOCOL

PROTEINS (LOWRY) PROTOCOL 1 PROTEINS (LOWRY) PROTOCOL 1. INTRODUCTION The Lowry Assay: Protein by Folin Reaction (Lowry et al., 1951) has been the most widely used method to estimate the amount of proteins (already in solution

More information

Measuring Cell Viability/Cytotoxicity: Cell Counting Kit-F

Measuring Cell Viability/Cytotoxicity: Cell Counting Kit-F Introduction The Cell Counting Kit-F is a fluorometic assay for the determination of viable cell numbers. Calcein-AM in this kit passes through the cell membrane and is hydrolized by the esterase in the

More information

Instructions and lab report for the practical lesson on biochemistry. Task 1: Qualitative estimation of protein in CSF (Pandy s test)

Instructions and lab report for the practical lesson on biochemistry. Task 1: Qualitative estimation of protein in CSF (Pandy s test) Date... Name... Group... Instructions and lab report for the practical lesson on biochemistry Topic: Biochemical examination of cerebrospinal fluid Task 1: Qualitative estimation of protein in CSF (Pandy

More information

Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan

Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan 489 18 Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan 18.1 Introduction Antibody catalysis is an experimental science. Although success in this field is

More information

MEF Nucleofector Kit 1 and 2

MEF Nucleofector Kit 1 and 2 page 1 of 7 MEF Nucleofector Kit 1 and 2 for Mouse Embryonic Fibroblasts (MEF) MEF display significant phenotypic variations which depend on the strain, the genetic background of the they are isolated

More information

Solutions: Molarity. A. Introduction

Solutions: Molarity. A. Introduction Solutions: Molarity. A. Introduction... 1 B. Molarity... 1 C. Making molar solutions... 2 D. Using molar solutions... 4 E. Other mole-based concentration units [optional]... 6 F. Answers... 7 A. Introduction

More information

Ion Selective Electrodes

Ion Selective Electrodes Ion Selective Electrodes OAKTON has a large selection of Ion Selective Electrodes () to suit a wide variety of applications. Each electrode has a typical response time of 20 to 30 seconds but will vary

More information

Quick Start Bradford Protein Assay

Quick Start Bradford Protein Assay Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) Table of Contents Section 1 Introduction 1 1.1 Principle

More information

Classic Immunoprecipitation

Classic Immunoprecipitation 292PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Classic Immunoprecipitation Utilizes Protein A/G Agarose for Antibody Binding (Cat.

More information

Glycolysis Cell-Based Assay Kit

Glycolysis Cell-Based Assay Kit Glycolysis Cell-Based Assay Kit Item No. 600450 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION

More information

EdU Flow Cytometry Kit. User Manual

EdU Flow Cytometry Kit. User Manual User Manual Ordering information: (for detailed kit content see Table 2) EdU Flow Cytometry Kits for 50 assays: Product number EdU Used fluorescent dye BCK-FC488-50 10 mg 6-FAM Azide BCK-FC555-50 10 mg

More information

竞 争 性 分 析 Epitope Mapping 实 验 方 法

竞 争 性 分 析 Epitope Mapping 实 验 方 法 竞 争 性 分 析 Epitope Mapping 实 验 方 法 ABSTRACT The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can

More information

Lipid Extraction Kit (Chloroform Free), Trial Size

Lipid Extraction Kit (Chloroform Free), Trial Size Product Manual Lipid Extraction Kit (Chloroform Free), Trial Size Catalog Number STA-612-T 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipids are a diverse group of

More information

Emergency Medications Approved for Use at VAPAHCS

Emergency Medications Approved for Use at VAPAHCS Table 1: Medications (PAD GM&S) Emergency Medications Approved for Use at VAPAHCS PAD GM&S (See HCSM 11-12-35 Attachment A CPR Committee Atropine 1mg/10ml syringe X 3 Normal saline 1000ml bag X 2 Sodium

More information

Peptide Antibody Production

Peptide Antibody Production Peptide Antibody Production A) Peptide BioSynthesis (http://www.biosyn.com, 800-227-0627) B) Conjugation of peptide to KLH (Imject Maleimide Activated KLH, PIERCE=Thermo #77605, 10 mg) C) Peptide affinity

More information

STANDARD OPERATING PROCEDURE

STANDARD OPERATING PROCEDURE STANDARD OPERATING PROCEDURE Title: Antibody Production at Strategic Diagnostics Inc. SOP#: M-119 Version #: 1 Date Approved: August 6, 2009 Author: Strategic Diagnostic Inc. Date Modified: 1. PURPOSE

More information

CD3/TCR stimulation and surface detection Determination of specificity of intracellular detection of IL-7Rα by flow cytometry

CD3/TCR stimulation and surface detection Determination of specificity of intracellular detection of IL-7Rα by flow cytometry CD3/TCR stimulation and surface detection Stimulation of HPB-ALL cells with the anti-cd3 monoclonal antibody OKT3 was performed as described 3. In brief, antibody-coated plates were prepared by incubating

More information

Protocol: HPLC (amino acids)

Protocol: HPLC (amino acids) Page 1 of 6 1. Chemicals Composition M MW gram volume (ml) product code 0-pthaldialdehyde C 8H 6O 2 134.13 Sigma P0657 Perchloric acid HClO 4 3.5 100.46 Dilute 70% stock solution 1:1 with demi to obtain

More information

CHEF Genomic DNA Plug Kits Instruction Manual

CHEF Genomic DNA Plug Kits Instruction Manual CHEF Genomic DNA Plug Kits Instruction Manual Catalog Numbers 170-3591 170-3592 170-3593 For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) Table of

More information

Gel Filtration Standard

Gel Filtration Standard Gel Filtration Standard Instruction Manual Catalog # 151-1901 Table of Contents Section 1 Introduction... 1 1.1 Instructions... 1 1.2 Recommended Volume of Standard... 2 1.3 Shelf Life... 5 1.4 Storage...

More information

TOTAL PROTEIN FIBRINOGEN

TOTAL PROTEIN FIBRINOGEN UNIT: Proteins 16tproteins.wpd Task Determination of Total Protein, Albumin and Globulins Objectives Upon completion of this exercise, the student will be able to: 1. Explain the ratio of albumin and globulin

More information

Calculation of Molar Masses. Molar Mass. Solutions. Solutions

Calculation of Molar Masses. Molar Mass. Solutions. Solutions Molar Mass Molar mass = Mass in grams of one mole of any element, numerically equal to its atomic weight Molar mass of molecules can be determined from the chemical formula and molar masses of elements

More information

Laboratory Techniques I Oncology for Scientists I

Laboratory Techniques I Oncology for Scientists I Laboratory Techniques I Oncology for Scientists I September 9 th, 2015 Hayley Affronti, PhD Student Hayley.Affronti@roswellpark.org Dr. Sheila Figel too! When we first hit the lab there are so many things

More information

Analysis of Casein and Whey Protein in Whole, 2%, and Skim Milk by Capillary Gel Electrophoresis

Analysis of Casein and Whey Protein in Whole, 2%, and Skim Milk by Capillary Gel Electrophoresis Analysis of Casein and Whey Protein in Whole, 2%, and Skim Milk by Capillary Gel Electrophoresis Marcia Santos, Staff Applications Scientist, Beckman Coulter Life Sciences, Brea, CA USA Mark Lies, Marketing

More information

DNA SPOOLING 1 ISOLATION OF DNA FROM ONION

DNA SPOOLING 1 ISOLATION OF DNA FROM ONION DNA SPOOLING 1 ISOLATION OF DNA FROM ONION INTRODUCTION This laboratory protocol will demonstrate several basic steps required for isolation of chromosomal DNA from cells. To extract the chromosomal DNA,

More information

Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method)

Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method) Standard Operating Procedure for Total Kjeldahl Nitrogen (Lachat Method) Grace Analytical Lab 536 South Clark Street 10th Floor Chicago, IL 60605 April 15, 1994 Revision 2 Standard Operating Procedure

More information

Human Peripheral Blood Mononuclear Cell (PBMC) Manual

Human Peripheral Blood Mononuclear Cell (PBMC) Manual Human Peripheral Blood Mononuclear Cell (PBMC) Manual INSTRUCTION MANUAL ZBM0063.04 SHIPPING CONDITIONS Human Peripheral Blood Mononuclear Cells, cryopreserved Cryopreserved human peripheral blood mononuclear

More information

Below is a list of things you should be aware of before you schedule your sort.

Below is a list of things you should be aware of before you schedule your sort. Sorting at the Flow Cytometry Facility At the present time, the assistance of a trained cell sorter operator is needed for all sorting applications. As such, if you are planning a first time sort you will

More information

Catalase Assay Kit. Catalog Number CAT100 Storage Temperature 2 8 C TECHNICAL BULLETIN

Catalase Assay Kit. Catalog Number CAT100 Storage Temperature 2 8 C TECHNICAL BULLETIN Catalase Assay Kit Catalog Number CAT100 Storage Temperature 2 8 C TECHNICAL BULLETIN Product Description Catalase is an antioxidant enzyme ubiquitously present in mammalian and non-mammalian aerobic cells

More information

Optimized Protocol sirna Test Kit for Cell Lines and Adherent Primary Cells

Optimized Protocol sirna Test Kit for Cell Lines and Adherent Primary Cells page 1 of 8 sirna Test Kit for Cell Lines and Adherent Primary Cells Kit principle Co-transfection of pmaxgfp, encoding the green fluorescent protein (GFP) from Pontellina p. with an sirna directed against

More information

Design Contents and Equipment for the Cell Culture Laboratory

Design Contents and Equipment for the Cell Culture Laboratory Design Contents and Equipment for the Cell Culture Laboratory 1.0 Introduction... 4 2.0 Design and Equipment for the Cell Culture Laboratory... 4 2.1 Laboratory Design... 4 2.2 Microbiological Safety Cabinets...

More information

Cell Culture Experiments

Cell Culture Experiments Cell Culture Experiments Cell cultures are another way to observe and analyze biological systems. These in vitro (in glass; outside the living body and in an artificial environment) models allow experimental

More information

Preparation of frequently used solutions

Preparation of frequently used solutions Preparation of frequently used solutions Content 1. Diluting Concentrated Acids (Last Login: 08/08/2009) 2. Indicators (Last Login: 27/07/2009) 3. Standard Buffer Solutions (Last Login: 27/07/2009) 4.

More information

Blood Tubes and Lancets 1-800-962-9888

Blood Tubes and Lancets 1-800-962-9888 Blood Tubes and Lancets 1-800-962-9888 1-800-962-9888. Table of Contents BLOOD COLLECTION MONOJECT Blood Collection Products Safety Lancet... 4 Lancet... 4 Corvac Integrated Serum Separator Tubes Red/Grey

More information

Instructions. Torpedo sirna. Material. Important Guidelines. Specifications. Quality Control

Instructions. Torpedo sirna. Material. Important Guidelines. Specifications. Quality Control is a is a state of the art transfection reagent, specifically designed for the transfer of sirna and mirna into a variety of eukaryotic cell types. is a state of the art transfection reagent, specifically

More information

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit

Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Rat creatine kinase MM isoenzyme (CK-MM) ELISA Kit Catalog Number. CSB-E14405r For the quantitative determination of rat creatine kinase MM isoenzyme (CK-MM) concentrations in serum, plasma and tissue

More information

Protein Stabilization Reagents. Elevated Protein Protection

Protein Stabilization Reagents. Elevated Protein Protection Protein Stabilization Reagents Elevated Protein Protection Protein Stabilization Reagents Naturally occurring proteases and phosphatases can destroy proteins you spent days isolating. Save valuable time

More information

Protocol for Preparation of Cell-Free Stocks of TCLA HIV-1 in Cell Lines (Montefiori Lab) July 2007

Protocol for Preparation of Cell-Free Stocks of TCLA HIV-1 in Cell Lines (Montefiori Lab) July 2007 Protocol for Preparation of Cell-Free Stocks of TCLA HIV-1 in Cell Lines (Montefiori Lab) July 2007 I. INTRODUCTION During the course of investigating the neutralizing antibody response in recipients of

More information

ELECTROLYTE SOLUTIONS (Continued)

ELECTROLYTE SOLUTIONS (Continued) ELECTROLYTE SOLUTIONS (Continued) Osmolarity Osmotic pressure is an important biologic parameter which involves diffusion of solutes or the transfer of fluids through semi permeable membranes. Per US Pharmacopeia,

More information

Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum

Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum Flowers and Vaillancourt, 2005. Current Genetics 48: 380-388 NOTE added by L. Vaillancourt:

More information

Amaxa Mouse/Rat Hepatocyte Nucleofector Kit

Amaxa Mouse/Rat Hepatocyte Nucleofector Kit Amaxa Mouse/Rat Hepatocyte Nucleofector Kit For Primary Mouse and Rat Hepatocytes This protocol is designed for freshly isolated mouse and rat hepatocytes; polygonal, adherent cells Example for Nucleofection

More information