8:15-8:55 REGISTRATION 9:00-9:15 OPENING 9:15-9:55 MIGUEL ANDRADE. 9:55-10:35 UNAI SILVÁN

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1 3rd Alumni Research Meeting Alicia Alonso and Ana Zubiaga (organizers) Paraninfo Faculty of Science and Technology UPV/EHU Leioa Campus Sponsored by Basque Government, Vicerrectorado del Campus de Bizkaia, Faculty of Science and Technology Biophysics Unit (CSIC-UPV/EHU) 8:15-8:55 REGISTRATION 9:00-9:15 OPENING PROGRAMME 9:15-9:55 MIGUEL ANDRADE. Max Delbrück Center for Molecular Medicine, Berlin "Data and text mining for the analysis of protein-protein interaction networks". 9:55-10:35 UNAI SILVÁN Uniklinik Balgrist, Institute für Biomechanics. ETH and University of Zürich (Switzerland). The organization of actin cytoskeleton". 10:35-11:15 MARÍA SOLEDAD SANTISTEBAN. University of North Carolina at Pembroke, USA. "Histone variant H2A.Z role in transcription" 11:15-11:45 Coffee

2 11:50-12:30 Eduardo Rial CIB Centro Investigaciones Biológicas- CSIC, Madrid. "Oxidative stress, thermogenesis and evolution of the uncoupling proteins" 12:30-13:10 Guillermo Montoya CNIO Centro Nacional Investigaciones Oncológicas, Madrid. "Molecular machines involved in protein folding and DNA unwinding or how to spend ATP for a good cause " 13:30-13:30 Concluding remarks 14:00 Lunch-colloquium (limited seats available) Certificates of attendance will be distributed at the end of the event. Registration required. No registration fee. For registration, please, send an to: (Specify subject: BioForo) providing the following information: Name, professional status (undergradute student, PhD student, professor.), working address, director, and name and address of supervisor (when appropriate), interested in attending lunch (yes/no). INTRODUCTION AND SPEAKERS PROFILE As in previous years, we offer a Special Bioforo meeting in December designed to show the research that our former students are carrying out outside the UPV/EHU. This year our speakers come from different countries (Germany, Switzerland, Spain and USA) and work in different areas in Biology: Bioinformatics, Cell Mechanics, Molecular Biology, Bioenergetics, and Structural Biology. Miguel Andrade He is interested in exploring gene function using computational techniques including new algorithms and the use of existing databases. He has a focus on studying the mechanisms involved in human disease with the goal of facilitating the development of better therapies. The results of his work are often distributed as software or online web tools. Take a look at the group members' publications or at their projects page for more information.

3 Contact details and individual web pages can be accessed from the Lab Members page. Selected Publications: McNeill B, Perez-Iratxeta C, Mazerolle C, Furimsky M, Mishina Y, Andrade-Navarro MA, Wallace VA. Comparative genomics identification of a novel set of temporally regulated hedgehog target genes in the retina. Mol Cell Neurosci Mar;49(3): Schaefer MH, Wanker EE, Andrade-Navarro MA. Evolution and function of CAG/polyglutamine repeats in protein-protein interaction networks. Nucleic Acids Res May 1;40(10): Muro EM, Mah N, Moreno-Hagelsieb G, Andrade-Navarro MA. The Pseudogenes of Mycobacterium leprae reveal the functional relevance of gene order within operons. Nucleic Acids Res Mar;39(5): Krzyzanowski, P.M., F.D. Price, E.M. Muro, M.A. Rudnicki, M.A. Andrade Navarro Integration of EST data and predicted RNA secondary structures facilitates novel ncrna discovery. PLoS One. 6:e Zhang, J., P. Wagh, D. Guay, L. Sanchez-Pulido, B.K. Padhi, V. Korzh, M.A. Andrade-Navarro and M. Akimenko Loss of fish actinotrichia proteins and the fin-to-limb transition. Nature. 466, Bröske AM, Vockentanz L, Kharazi S, Huska MR, Mancini E, Scheller M, Kuhl C, Enns A, Prinz M, Jaenisch R, Nerlov C, Leutz A, Andrade-Navarro MA, Jacobsen SE, Rosenbauer F. DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction. Nat Genet Nov;41(11): Unai Silván de Pedro Bachelor in Biochemistry UPV/EHU (2001), PhD in Biochemistry UPV/EHU (2008) Thesis advisors: Professor Dr. Juan Aréchaga and Professor Dr. Jon Arluzea. He moved in 2008 as a Postdoc to the the Müller Institute, Biozentrum of the University of Basel, Basel (Switzerland) where he started to work on Cell Mechanics applying a combination of biochemical methods with light microscopy, electron microscopy and atomic force microscopy (AFM). In 2012 he was appointed Senior Postdoc at the Uniklinik Balgrist, Institute of Biomechanics, ETH and University of Zürich (Switzerland). Selected Publications: Silván U, Boiteux C, Sütterlin R, Schroeder U, Mannherz HG, Jockusch BM, Bernèche S, Aebi U, Schoenenberger CA. An antiparallel actin dimer is associated with the endocytic pathway in mammalian cells. J Struct Biol Jan;177(1): Silván U, Díez-Torre A, Andrade R, Arluzea J, Silió M, Aréchaga J. Embryonic stem cell transplantation into seminiferous tubules: a model for the study of invasive germ cell tumors of the testis. Cell Transplant. 2011;20(5): Díez-Torre A, Silván U, Díaz-Núñez M, Aréchaga J. The role of

4 microenvironment in testicular germ cell tumors. Cancer Biol Ther Sep;10(6): Silván U, Arlucea J, Andrade R, Díez-Torre A, Silió M, Konerding MA, Aréchaga J. Angiogenesis and vascular network of teratocarcinoma from embryonic stem cell transplant into seminiferous tubules. Br J Cancer Jul 7;101(1): Schroeder U, Graff A, Buchmeier S, Rigler P, Silvan U, Tropel D, Jockusch BM, Aebi U, Burkhard P, Schoenenberger CA. Peptide nanoparticles serve as a powerful platform for the immunogenic display of poorly antigenic actin determinants. J Mol Biol Mar 13;386(5): María Soledad Esteban. Assistant Professor Department of Biology, University of North Carolina at Pembroke. Her current research interest is Yeast Molecular Genetics, Epigenetic Regulation of Eukaryotic Gene Expression.In the eukaryotic cell nucleus, DNA is packaged with histones into nucleosomes, the repeating subunits of chromatin. The precise organization of DNA in chromatin has important functional consequences. DNA-template processes such as transcription, replication and chromosome segregation are dependent on the remarkable packaging of the DNA in chromatin. The long-range objective of her research is to understand the molecular mechanisms of chromatin-regulated gene activation. Her research has primarily focused on the role of histone H2A She has discovered that Htz1 functions to enable transcription by RNA pol II. Specifically, her data indicate that Htz1 possibly plays a role in elongation. She continues exploring this hypothesis using techniques of molecular genetics that will involve mutational analysis, genetic screens, chromatin immunoprecipitation, microarray analysis, real-time PCR, etc. Selected publications: Santisteban MS, Hang M, Smith MM. Histone variant H2A.Z and RNA polymerase II transcription elongation. Mol Cell Biol May;31(9): Jensen K, Santisteban MS, Urekar C, Smith MM. Histone H2A.Z acid patch residues required for deposition and function. Mol Genet Genomics Apr;285(4): Santisteban MS, Kalashnikova T, Smith MM. Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes. Cell Oct 27;103(3): Glowczewski L, Yang P, Kalashnikova T, Santisteban MS, Smith MM. Histone-histone interactions and centromere function. Mol Cell Biol Aug;20(15): histone protein is encoded by the HTZ1 gene.

5 Eduardo Rial. His main interest is Mitochondrial Bioenergetics The mitochondrial oxidative phosphorylation embraces the reactions that allow ATP synthesis using the energy made available from substrate oxidation at the respiratory chain. The two processes are coupled through the proton gradient generated during the transfer of electrons from the substrates to oxygen. His group investigates mechanisms that modulate the energetic efficiency of the process. The uncoupling proteins (UCPs) are carriers of the mitochondrial inner membrane whose biological function is to allow a regulated discharge of the proton gradient. There are a large number of processes that appear to involve the UCPs. For example, this energy dissipatory mechanism is used by mammals to maintain body temperature when cold exposed or to burn excess calories ingested with the diet. Since the UCPs catalyze the re-entry of protons into the mitochondrial matrix, they cause an increase in the rate of respiration that leads to a decrease in the production of reactive oxygen species (ROS) and therefore the UCPs are an element of the cellular defenses against oxidative stress. Thus, the uncoupling protein UCP2, which is found in many tissues and organs, is over-expressed in pathological processes in which ROS play an important role (atherosclerosis, cancer, chronic inflammation, etc.). Over the past few years, genes coding for UCPs have been described not only in animals but also in plants and even in unicellular organisms. The ubiquitous presence of the UCPs suggests that physiological uncoupling of oxidative phosphorylation is a general strategy adopted by living organisms to regulate the energetic efficiency and, for example, modulate the mitochondrial production of ROS. The group has been investigating for nearly thirty years the physiological role and the molecular mechanisms of transport and regulation of the uncoupling proteins UCP1 and UCP2. HOME PAGE Selecteds publications: Ortega-Molina A, Efeyan A, Lopez-Guadamillas E, Muñoz-Martin M, Gómez López G, Cañamero M, Mulero F, Pastor J, Martinez S, Romanos E, González- Barroso MM, Rial E, Valverde AM, Bischoff JR, Serrano M [2012] Pten positively regulates brown adipose function, energy expenditure, and longevity. Cell Metab 15, Luévano-Martínez LA, Barba-Ostria C, Araiza-Olivera D, Chiquete-Félix N, Guerrero-Castillo S, Rial E, Georgellis D, Uribe-Carvajal S [2012] A critical tyrosine residue of the mitochondrial oxaloacetate carrier determines its uncoupling protein (UCP)-like function in yeast. Biochem J 443,

6 Rial E, Rodríguez-Sánchez L, Gallardo-Vara E, Zaragoza P, Moyano E, González-Barroso MM [2010] Lipotoxicity, Fatty Acid Uncoupling and Mitochondrial Carrier Function. Biochim Biophys Acta Bioenerg 1797: Redondo-Horcajo M, Romero N, Martínez-Acedo P, Martínez-Ruiz A, Quijano C, Lourenço CF, Movilla N, Enríquez JA, Rodríguez-Pascual F, Rial E, Radi R, Vázquez J, Lamas S [2010] Cyclosporine A-induced nitration of tyrosine 34 MnSOD in endothelial cells: role of mitochondrial superoxide. Cardiovasc Res 87: Delás J, Notari M, Forés J, Pechuan J, Porcar M, Navarro E, Montagud A, Baguena M, Peretó J, Córdoba PF, González-Barroso MM, Rial E, Moya A, Urchueguía J [2009] Yeast cultures with UCP1 uncoupling activity as a heating device. N Biotechnol 26: González-Barroso MM, Rial E [2009] The role of fatty acids in the activity of the uncoupling proteins. Curr Chem Biol 3: Guillermo Montoya Guillermo Montoya was born in Madrid (Spain) in 1967 and obtained his Bachelor degree in Biochemistry from the Universidad del País Vasco in 1990, and his PhD in Chemistry from the Universidad de Zaragoza in He obtained both a European Molecular Biology Organisation (EMBO) and a Federation of European Biochemical Societies (FEBS) Fellowship and moved to the Max Planck-Institut für Biophysik in Frankfurt am Main (Germany), where he worked on membrane protein crystallisation in the group of the Nobel Laureate H. Michel. Montoya later obtained both an EMBO long-term and a Marie Curie Fellowship and spent nine years at the European Molecular Biology Laboratory (EMBL) in Heidelberg (Germany), working in I. Sinning s Group where he focused on the crystallisation of the cytocrome bc1 membrane protein complex and later pioneered the study of the structure of the signal recognition particle (SRP), an essential ribonucleoprotein complex involved in protein targeting. In 1998 he was appointed as Researcher at the Consejo Superior de Investigaciones Científicas (CSIC) and was awarded a Peter und Traudl Engelhorn Foundation Research Fellowship. Since 2003 he has been an Honorary Professor in Biochemistry at the Universidad Autónoma de Madrid and Member of the working group in charge of the design of the biocrystallography beamline at the Spanish Synchrotron (ALBA). Montoya has been Head of the CNIO s Macromolecular Crystallography Group since February 2002 and was acting Director of the Structural Biology and Biocomputing Programme from November 2003 to January In 2009 he was awarded the National Prizes of the Fundación Mutua Madrileña and the Fundación Caja Rural de Granada-Ministerio de Sanidad. Selected Publications:

7 Carranza G, Castaño R, Fanarraga ML, Villegas JC, Gonçalves J, Soares H, Avila J, Marenchino M, Campos-Olivas R, Montoya G, Zabala JC. Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics. Cell Mol Life Sci Sep 1. Valton J, Daboussi F, Leduc S, Molina R, Redondo P, Macmaster R, Montoya G, Duchateau P. 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases. J Biol Chem Aug 31;287(36): Molina R, Redondo P, Stella S, Marenchino M, D'Abramo M, Gervasio FL, Charles Epinat J, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G. Non-specific protein-dna interactions control I-CreI target binding and cleavage. Nucleic Acids Res Aug;40(14): Sanchez-Berrondo J, Mesa P, Ibarra A, Martínez-Jiménez MI, Blanco L, Méndez J, Boskovic J, Montoya G. Molecular architecture of a multifunctional MCM complex. Nucleic Acids Res Feb;40(3): Yébenes H, Mesa P, Muñoz IG, Montoya G, Valpuesta JM. Chaperonins: Two ringsfor folding. Trends Biochem Sci Aug;36(8): Epub 2011 Jun 30. Review. Muñoz IG, Yébenes H, Zhou M, Mesa P, Serna M, Park AY, Bragado Nilsson E,Beloso A, de Cárcer G, Malumbres M, Robinson CV, Valpuesta JM, Montoya G. Crystal structure of the open conformation of the mammalian chaperonin CCT in complex with tubulin. Nat Struct Mol Biol Jan;18(1):14-9. Fernández-Miranda G, Pérez de Castro I, Carmena M, Aguirre-Portolés C, Ruchaud S, Fant X, Montoya G, Earnshaw WC, Malumbres M. SUMOylation modulates the function of Aurora-B kinase. J Cell Sci Aug 15;123(Pt 16): Redondo P, Prieto J, Muñoz IG, Alibés A, Stricher F, Serrano L, Cabaniols JP, Daboussi F, Arnould S, Perez C, Duchateau P, Pâques F, Blanco FJ, Montoya G.Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases. Nature Nov 6;456(7218):