Electron Transfer Dissociation (ETD) Innovation with Integrity. Look Beneath the Surface. Ion Trap MS
|
|
- Arthur Cross
- 7 years ago
- Views:
Transcription
1 Electron Transfer Dissociation (ETD) Look Beneath the Surface Innovation with Integrity Ion Trap MS
2 Bruker Daltonics Leader in ETD Technology amazon Featuring ETD Since its commercial introduction in 2005, Bruker has been the leader in highly efficient and sensitive electron transfer dissociation (ETD) and proton transfer reaction (PTR) technology. We are constantly developing cutting-edge solutions for in-depth characterization of proteins and top-down sequencing analysis using our ETD technology. Using smart combinations of CID and ETD our customers obtain the greatest possible depth of information. Both complementary and confirmatory identification of post-translational modifications (PTM) provide you with the complete picture in challenging protein analyses.
3 ETD Applications From PTM to Top-Down Analysis As a complementary fragmentation technique to CID, ETD is of major importance for: Localization of PTMs, such as phosphorylation, sulfonation, and nitrosylation Analysis of O- and N-glycopeptides Characterization of disulfide linkages Identification of non-tryptic peptides, such as those with multiple basic residues Determination of protein termini by top-down sequencing Nebulizer High-transmission dual ion funnel transfer line Ion guide In vacuum RF detection Ion trap High energy conversion detector Fast acquisition circuitry Off-axis geometry Vacuum Stage 1 Reagent anion source Fast-scanning analyzer Stage 2 Stage 3 Stage 4 Atmospheric- Pressure Ion Source
4 ETD and PTR on the amazon Technology Leadership Key technical facts ETD cleaves N-Cα bonds producing c- and z-ions Labile bonds of PTMs remain intact The most efficient reagent Fluoranthene is used for both ETD and PTR Highest sensitivity for low-abundance peptides on an LC timescale: Identification of 18 peptides with a Mascot score > 20 from 500 amol BSA on column The ETD reaction CID and ETD fragmentation The 3D advantage The 3D advantage of the spherical ion trap is the decisive factor for the highest efficiency ETD and PTR reactions: Robust and fast trapping of cations and anions for direct ETD reaction Better cross section for efficient ionion-reactions in 3D ion traps due to compression into the same small globular volume Roepstorff nomenclature scheme The 3D advantage ESI source Dual Ion funnel Reagent anion source 3D Ion Trap
5 PTM Analysis A Must for Protein Characterization Complete PTM characterization The regulatory function of many proteins is controlled by reversible PTMs such as sulfonation and phosphorylation. PTM analysis includes identification and unambiguous site localization. ETD is the method of choice for site-profiling of biologically important PTMs. In contrast to CID, ETD preserves the modification on the peptide backbone and therefore enables a definite assignment of the modification site. CID spectra generate significant neutral loss of sulfonation and phosphorylation (-SO 3 or -H 3 PO 4 ) and only a few lowabundance peptide fragments. Because it is a very labile PTM, sulfonation is especially difficult to analyze using CID. However, ETD supports peptide sequencing and site-localization of all PTMs and is therefore an invaluable tool for extensive protein characterization. Identification and localization of sulfonation Neutral loss of SO 3 detected in CID. Peptide sequence identification by ETD: ALAPE(sulfo)YAK. Identification and localization of phosphorylation Neutral loss of H 3 PO 4 detected in CID. Peptide sequence identification and PTM localization by ETD: THTTALAGR(phospho)SP(phospho)SPASGR.
6 Complete Glycopeptide Analysis by Fragment Triggered ETD Glycopeptide analysis is of particular interest in glycomics, because detailed information about glycan, peptide and glycosylation site is needed to understand relationships between structure, location and function. Localization of O-glycosylation sites Tools for in-depth characterization of N- and O-glycopeptides: CaptiveSpray nanobooster Dramatic enhancement of glycopeptide ionization and shift to higher charge states for highest ETD spectra quality CID Identification of glycan composition and structure with GlycoQuest ETD Sequencing of glycopeptides and localization of glycosylation sites Fragment triggered ETD enables targeted glycopeptide profiling. ETD is only triggered if diagnostic oxonium ions have been detected in the preceding CID spectrum. This allows for simultaneous protein identification and effective glycopeptide profiling. Structure elucidation of an EPO O-glycopeptide (precursor [M+3H] 3+ = m/z). Top: CID spectrum; Bottom: ETD spectrum with assignment of glycosylated serine S *. Localization of N-glycosylation sites Serotransferrin N-glycopeptide profiling using Fragment triggered ETD (precursor [M+3H] 3+ = m/z). The glycosylated asparagine is marked with an asterisk.
7 ETD-Based Mapping of Disulfide Bonds Disulfide linkage of cysteine residues is an important PTM with regards to protein folding, structure, and biological function. Preferred disulfide bond cleavage by ETD Because it favors the cleavage of S-S bonds, ETD is key to disulfide linkage analysis. After cleavage, the sequences of the resulting peptides can be determined by CID MS 3. The figure on the right shows MS and ETD MS 2 spectra of a pair of interchain disulfide-bound peptides from a monoclonal IgG2. Precursor ions with charge states up to 4+ are generated with the CaptiveSpray nanobooster source. MS and ETD MS 2 spectra of linked peptides The major fragments m/z and result from the cleavage of the disulfide bond and are used as precursor ions for the following CID fragmentation. CID data allow sequencing of the peptides as shown for SFNRGEC from the light chain. The amazon speed ETD supports a variety of different workflows, ranging from a completely untargeted, data dependent MS 3 to a fully targeted smartmrm approach with predefined precursors for ETD and CID. LC: light chain, HC: heavy chain Peptide sequencing by CID Peptide sequence SFNRGEC from the IgG2 light chain identified by CID MS 3.
8 Top-Down Protein Characterization by ETD-Analysis of Hemoglobin Sequence variant analysis of full-length hemoglobin ETD fragmentation of full-length HbA -chain Until now, sequence variant analysis of full-length hemoglobin has been a major challenge. ETD fragmentation after precise isolation of the multiply charged Hb chains (for example, -chain 19+ charged) generates specific fragments that enable an unambiguous identification of the Hb specific sequence variant. Typical extracted ion chromatograms Hb A Control Hb C Homozygote Hb S Homozygote Typical Extracted Ion Chromatograms (EIC) of a control (HbA) and a homozygote Hemoglobin C and S sample. Several sequencespecific ETD fragments are used to generate the EIC traces for the different Hb chain variants.
9 Technical Specifications Technology-driven solutions Patented SmartICC for optimal ion storage SmartFrag Enhanced for reproducible high-quality MS/MS spectra generation and confident library searches Panorama fragmentation (PAN) enabling CID fragmentation without 1/3 cut-off MS/MS modes Highly efficient and sensitive ETD/PTR capabilities for PTM detection and top-down sequencing Data-dependent automs n Scheduled precursor list (SPL) supporting ETD and CID MS n in positive and negative mode SmartMRM for highly sensitive SPL - based targeted experiments Neutral-loss and fragment-triggered experiments for the analysis of posttranslational modifications (by ETD) Manual MS n up to MS 11 in all scan modes for structural elucidation Source options CaptiveSpray nanobooster: robust and reliable plug-and-play nano/cap ESI source for flow rates from nl/min ESI, APCI, APPI, and DIP source for direct analysis of solids and liquids Advion Triversa NanoMate Smart CE-MS coupling with grounded spray needle Software solutions ProteinScape including GlycoQuest glycan database search engine RapiDeNovo for de-novo sequencing of proteins ProfileAnalysis for statistical comparison of metabolite and proteomic profiles Compass OpenAccess client/serverbased system for walk-up users Compass Security Pack for regulated environments Scan speed and resolution Scan Mode Resolution u/sec m/z XtremeScan 2+ ions 52, UltraScan 3+ ions 32, Enhanced Resolution 4+ ions Maximum Resolution 8+ ions Extended Mass Range 27,
10 Bruker Daltonics is continually improving its products and reserves the right to change specifications without notice. BDAL , Further Reading Bruker brochures CaptiveSpray nanobooster The revolution in Proteomics Ionization Glyco Analysis Solutions Expanding the frontier in Glycobiology amazon speed Ion Trap Performance Beyond Imagination Bruker Application Notes App-Note LCMS-57 In-depth Characterization of Neutral and Acidic Glycopeptides by ZIC-HILIC Enrichment and Mass Spectrometry Jessica Wohlgemuth, Sven Andrecht, Merck KGaA, Darmstadt, Germany; Andrea Schneider, Anja Resemann, Arndt Asperger, Bruker Daltonik GmbH, Bremen, Germany (2010) App-Note LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities Kristina Neue, Andrea Kiehne, Markus Meyer, Marcus Macht, Ulrike Schweiger-Hufnagel, Anja Resemann; Bruker Daltonik GmbH, Bremen, Germany (2012) App-Note LCMS-71 Identification of Proteins from a HeLa Cell Culture Sample Using the amazon speed ETD system Zoltan Czentnar, Andrea Kiehne, Kristina Neue, Markus Meyer; Bruker Daltonik GmbH, Bremen, Germany (2012) App-Note LCMS-75 Identification and Characterization of PTMs from a Complex Peptide Mixture Using the amazon speed ETD Anjali Alving, Matthew Willetts (2012) App-Note LCMS-76 Identification of a Second N-glycosylation Site in a Human PSA Sample by Combined CID/ETD Fragmentation Kristina Marx, Anja Resemann, Ulrike Schweiger-Hufnagel, Waltraud Evers, Andrea Kiehne, Markus Meyer; Bruker Daltonik GmbH, Bremen, Germany (2012) App-Note LCMS-93 amazon speed ETD: Exploring glycopeptides in protein mixtures using Fragment Triggered ETD and CaptiveSpray nanobooster Kristina Marx, Andrea Kiehne, Markus Meyer; Bruker Daltonik GmbH, Bremen, Germany (2014) For research use only. Not for use in diagnostic procedures. Bruker Daltonik GmbH Bremen Germany Phone +49 (0) Fax +49 (0) Bruker Daltonics Inc. Billerica, MA USA Phone +1 (978) Fax +1 (978) ms.sales.bdal@bruker.com -
ProteinScape. Innovation with Integrity. Proteomics Data Analysis & Management. Mass Spectrometry
ProteinScape Proteomics Data Analysis & Management Innovation with Integrity Mass Spectrometry ProteinScape a Virtual Environment for Successful Proteomics To overcome the growing complexity of proteomics
More informationamazon SL Innovation with Integrity Setting New Standards in Performance, Simplicity and Value Ion Trap MS
amazon SL Setting New Standards in Performance, Simplicity and Value Innovation with Integrity Ion Trap Best-In-Class Ion Trap Mass Spectrometer for Routine Analysis The amazon SL entry-level system is
More informationApplication Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities
Application Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities Introduction Glycosylation is one of the most common and
More informationApplication Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method
Application Note # LCMS-81 Introducing New Proteomics Acquisiton Strategies with the compact Towards the Universal Proteomics Acquisition Method Introduction During the last decade, the complexity of samples
More informationprime Innovation with Integrity The multidimensional path to the Proteome Mass Spectrometry
prime The multidimensional path to the Proteome Innovation with Integrity Mass Spectrometry Reach for the Full Potential of Proteomics. Open Your Eyes to PRIME The Proteome is far more complex than was
More informationApplication Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software
Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Abstract Presented here is a case study of a walk-up liquid chromatography
More informationThermo Scientific PepFinder Software A New Paradigm for Peptide Mapping
Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small
More information# LCMS-35 esquire series. Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water
Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system
More informationApplication Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow
Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Abstract There is high demand in clinical research and forensic toxicology for comprehensive,
More informationEffects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests
Effects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests AB SCIEX TOF/TOF 5800 System with DynamicExit Algorithm and ProteinPilot Software for Robust Protein
More informationIn-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates
In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics
More informationProteinPilot Report for ProteinPilot Software
ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Pow erful mass spectrometers like
More informationBruker ToxScreener TM. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS
Bruker ToxScreener TM A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integrity UHR-TOF MS ToxScreener - Get the Complete Picture Forensic laboratories are frequently required
More informationMaster course KEMM03 Principles of Mass Spectrometric Protein Characterization. Exam
Exam Master course KEMM03 Principles of Mass Spectrometric Protein Characterization 2010-10-29 kl 08.15-13.00 Use a new paper for answering each question! Write your name on each paper! Aids: Mini calculator,
More informationAB SCIEX TOF/TOF 4800 PLUS SYSTEM. Cost effective flexibility for your core needs
AB SCIEX TOF/TOF 4800 PLUS SYSTEM Cost effective flexibility for your core needs AB SCIEX TOF/TOF 4800 PLUS SYSTEM It s just what you expect from the industry leader. The AB SCIEX 4800 Plus MALDI TOF/TOF
More informationMultiQuant Software 2.0 for Targeted Protein / Peptide Quantification
MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification Gold Standard for Quantitative Data Processing Because of the sensitivity, selectivity, speed and throughput at which MRM assays can
More informationApplication Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008
Bruker Daltonics Application Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008 In the ABRF-PRG study 2008 [*] the ability to characterize
More informationElectrospray Ion Trap Mass Spectrometry. Introduction
Electrospray Ion Source Electrospray Ion Trap Mass Spectrometry Introduction The key to using MS for solutions is the ability to transfer your analytes into the vacuum of the mass spectrometer as ionic
More informationOverview. Triple quadrupole (MS/MS) systems provide in comparison to single quadrupole (MS) systems: Introduction
Advantages of Using Triple Quadrupole over Single Quadrupole Mass Spectrometry to Quantify and Identify the Presence of Pesticides in Water and Soil Samples André Schreiber AB SCIEX Concord, Ontario (Canada)
More informationSimGlycan Software*: A New Predictive Carbohydrate Analysis Tool for MS/MS Data
SimGlycan Software*: A New Predictive Carbohydrate Analysis Tool for MS/MS Data Automated Data Interpretation for Glycan Characterization Jenny Albanese 1, Matthias Glueckmann 2 and Christof Lenz 2 1 AB
More informationaurora ICP-MS Innovation with Integrity The Science of Sensitivity ICP-MS
aurora ICP-MS The Science of Sensitivity Innovation with Integrity ICP-MS Powers the Business of Quantification aurora ICP-MS enhanced value package Bruker s ICP-MS is famous for its innovative and unique
More informationIncreasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays
Increasing the Multiplexing of High Resolution Targeted Peptide Quantification Assays Scheduled MRM HR Workflow on the TripleTOF Systems Jenny Albanese, Christie Hunter AB SCIEX, USA Targeted quantitative
More informationThe Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring
The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,
More informationPinpointing phosphorylation sites using Selected Reaction Monitoring and Skyline
Pinpointing phosphorylation sites using Selected Reaction Monitoring and Skyline Christina Ludwig group of Ruedi Aebersold, ETH Zürich The challenge of phosphosite assignment Peptides Phosphopeptides MS/MS
More informationSimGlycan Software*: A New Predictive Carbohydrate Analysis Tool for MS/MS Data
SimGlycan Software*: A New Predictive Carbohydrate Analysis Tool for MS/MS Data Automated Data Interpretation for Glycan Characterization Jenny Albanese1, Matthias Glueckmann2 and Christof Lenz2 1Applied
More informationAdvantages of the LTQ Orbitrap for Protein Identification in Complex Digests
Application Note: 386 Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Rosa Viner, Terry Zhang, Scott Peterman, and Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA,
More informationTutorial for Proteomics Data Submission. Katalin F. Medzihradszky Robert J. Chalkley UCSF
Tutorial for Proteomics Data Submission Katalin F. Medzihradszky Robert J. Chalkley UCSF Why Have Guidelines? Large-scale proteomics studies create huge amounts of data. It is impossible/impractical to
More informationAiping Lu. Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn
Aiping Lu Key Laboratory of System Biology Chinese Academic Society APLV@sibs.ac.cn Proteome and Proteomics PROTEin complement expressed by genome Marc Wilkins Electrophoresis. 1995. 16(7):1090-4. proteomics
More informationimpact II Innovation with Integrity Get the full picture the first time UHR-TOF MS
impact II Get the full picture the first time Innovation with Integrity UHR-TOF MS Sensational Capabilities of impact II Optimize your LC-MS methods without compromising performance: impact II delivers
More informationProteomics in Practice
Reiner Westermeier, Torn Naven Hans-Rudolf Höpker Proteomics in Practice A Guide to Successful Experimental Design 2008 Wiley-VCH Verlag- Weinheim 978-3-527-31941-1 Preface Foreword XI XIII Abbreviations,
More informationWorkshop IIc. Manual interpretation of MS/MS spectra. Ebbing de Jong. Center for Mass Spectrometry and Proteomics Phone (612)625-2280 (612)625-2279
Workshop IIc Manual interpretation of MS/MS spectra Ebbing de Jong Why MS/MS spectra? The information contained in an MS spectrum (m/z, isotope spacing and therefore z ) is not enough to tell us the amino
More informationBiopharmaceutical Glycosylation Analysis
Biopharmaceutical Glycosylation Analysis Glycosylation Analysis: Product Offering Molecular model of erythropoietin with complex N-linked glycans. Courtesy of M.R Wormald and R.A Dwek, Oxford Glycobioloy
More informationSession 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists
Program Overview Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Session 2. Principles of Mass Spectrometry Session 3. Mass spectrometry based proteomics
More informationMRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics
MRMPilot Software: Accelerating MRM Assay Development for Targeted Quantitative Proteomics With Unique QTRAP and TripleTOF 5600 System Technology Targeted peptide quantification is a rapidly growing application
More informationAPI 3200 LC/MS/MS SYSTEM. Performance, productivity and value combined
API 3200 LC/MS/MS SYSTEM Performance, productivity and value combined API 3200 LC/MS/MS SYSTEM Reliability, reproducibility, and confidence in your data. With an unmatched heritage of technological innovation
More informationChallenges in Computational Analysis of Mass Spectrometry Data for Proteomics
Ma B. Challenges in computational analysis of mass spectrometry data for proteomics. SCIENCE AND TECHNOLOGY 25(1): 1 Jan. 2010 JOURNAL OF COMPUTER Challenges in Computational Analysis of Mass Spectrometry
More information泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics
泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 2014 Training Course Wei-Hung Chang ( 張 瑋 宏 ) ABRC, Academia
More informationFast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3
Fast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3 Hongwei Xie and Weibin Chen Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B
More informationResearch-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE
Research-grade Targeted Proteomics Assay Development: PRMs for PTM Studies with Skyline or, How I learned to ditch the triple quad and love the QE Jacob D. Jaffe Skyline Webinar July 2015 Proteomics and
More informationMass Spectrometry Based Proteomics
Mass Spectrometry Based Proteomics Proteomics Shared Research Oregon Health & Science University Portland, Oregon This document is designed to give a brief overview of Mass Spectrometry Based Proteomics
More informationProSightPC 3.0 Quick Start Guide
ProSightPC 3.0 Quick Start Guide The Thermo ProSightPC 3.0 application is the only proteomics software suite that effectively supports high-mass-accuracy MS/MS experiments performed on LTQ FT and LTQ Orbitrap
More informationSimultaneous qualitative and quantitative analysis using the Agilent 6540 Accurate-Mass Q-TOF
Simultaneous qualitative and quantitative analysis using the Agilent 654 Accurate-Mass Q-TOF Technical Overview Authors Pat Perkins Anabel Fandino Lester Taylor Agilent Technologies, Inc. Santa Clara,
More informationDRUG METABOLISM. Drug discovery & development solutions FOR DRUG METABOLISM
DRUG METABLISM Drug discovery & development solutions FR DRUG METABLISM Fast and efficient metabolite identification is critical in today s drug discovery pipeline. The goal is to achieve rapid structural
More informationNUVISAN Pharma Services
NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!
More informationGENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert1, Michel Lhermitte 1, Frederic Grisel 2 1
GENERAL UNKNOWN SCREENING FOR DRUGS IN BIOLOGICAL SAMPLES BY LC/MS Luc Humbert, Michel Lhermitte, Frederic Grisel Laboratoire de Toxicologie & Génopathologie, CHRU Lille, France Waters Corporation, Guyancourt,
More informationHRMS in Clinical Research: from Targeted Quantification to Metabolomics
A sponsored whitepaper. HRMS in Clinical Research: from Targeted Quantification to Metabolomics By: Bertrand Rochat Ph. D., Research Project Leader, Faculté de Biologie et de Médecine of the Centre Hospitalier
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Tim Griffin Associate Professor, BMBB Faculty Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s
More informationPep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics
Pep-Miner: A Novel Technology for Mass Spectrometry-Based Proteomics Ilan Beer Haifa Research Lab Dec 10, 2002 Pep-Miner s Location in the Life Sciences World The post-genome era - the age of proteome
More informationPesticide Analysis by Mass Spectrometry
Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine
More informationIdentification of Surfactants by Liquid Chromatography-Mass Spectrometry
Identification of Surfactants by Liquid Chromatography-Mass Spectrometry James Little, Eastman Chemical Company, Kingsport, TN American Society for Mass Spectrometry 2004 Nashville, TN 1 verview Surfactant
More informationEleanor Riches Waters Corporation, Manchester, UK INTRODUCTION
X E V O T Q MS W IT H AT MOS P H E RIC P R E S SU R E P HOT O IO NIZ AT IO N (A P P I) SOU RC E: T H E IONIZATION OF COMPOUNDS WITH DIVERSE STRUCTURES USING VITAMINS AS A MODEL Eleanor Riches Waters Corporation,
More informationLa Protéomique : Etat de l art et perspectives
La Protéomique : Etat de l art et perspectives Odile Schiltz Institut de Pharmacologie et de Biologie Structurale CNRS, Université de Toulouse, Odile.Schiltz@ipbs.fr Protéomique et Spectrométrie de Masse
More informationAMD Analysis & Technology AG
AMD Analysis & Technology AG Application Note 120419 Author: Karl-Heinz Maurer APCI-MS Trace Analysis of volatile organic compounds in ambient air A) Introduction Trace analysis of volatile organic compounds
More informationQuantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry
Quantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry Paula Ladwig, David Barnidge, Mindy Kohlhagen, John Mills, Maria Willrich, Melissa R. Snyder and David Murray
More informationUnique Software Tools to Enable Quick Screening and Identification of Residues and Contaminants in Food Samples using Accurate Mass LC-MS/MS
Unique Software Tools to Enable Quick Screening and Identification of Residues and Contaminants in Food Samples using Accurate Mass LC-MS/MS Using PeakView Software with the XIC Manager to Get the Answers
More informationHenry Shion, Robert Birdsall, Steve Cubbedge, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS
Development of Integrated Informatics Workflows for the Automated Assessment of Comparability for Antibody Drug Conjugates (ADCs) Using LC-UV and LC-UV/MS Henry Shion, Robert Birdsall, Steve Cubbedge,
More informationA Streamlined Workflow for Untargeted Metabolomics
A Streamlined Workflow for Untargeted Metabolomics Employing XCMS plus, a Simultaneous Data Processing and Metabolite Identification Software Package for Rapid Untargeted Metabolite Screening Baljit K.
More information(c) How would your answers to problem (a) change if the molecular weight of the protein was 100,000 Dalton?
Problem 1. (12 points total, 4 points each) The molecular weight of an unspecified protein, at physiological conditions, is 70,000 Dalton, as determined by sedimentation equilibrium measurements and by
More informationAppendix 5 Overview of requirements in English
Appendix 5 Overview of requirements in English This document is a translation of Appendix 4 (Bilag 4) section 2. This translation is meant as a service for the bidder and in case of any differences between
More informationGlobal and Discovery Proteomics Lecture Agenda
Global and Discovery Proteomics Christine A. Jelinek, Ph.D. Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences Middle Atlantic Mass Spectrometry Laboratory Global
More informationLaboration 1. Identifiering av proteiner med Mass Spektrometri. Klinisk Kemisk Diagnostik
Laboration 1 Identifiering av proteiner med Mass Spektrometri Klinisk Kemisk Diagnostik Sven Kjellström 2014 kjellstrom.sven@gmail.com 0702-935060 Laboration 1 Klinisk Kemisk Diagnostik Identifiering av
More informationApplication Note # MS-14 Fast On-site Identification of Drugs with the mobile GC/MS system E²M
Bruker Daltonics Application Note # MS- Fast n-site Identification of Drugs with the mobile GC/MS system E²M For the detection of drugs a couple of quick tests are available which were used by the police
More information1) Technical informations. - a) How does it work? - b) Purification - c) Quality Control. 2) Standard synthesis
1) Technical informations - a) How does it work? - b) Purification - c) Quality Control 2) Standard synthesis - a) Standard peptides - b) Modified peptides - c) Shipment and Delivery Time - d) How to order?
More informationElectrospray Mass Spectrometry of Tris-acetylacetonatochromium(III)
Electrospray Mass Spectrometry of Tris-acetylacetonatochromium(III) Purpose: Verify the synthesis of the synthesis product, Cr(acac) 3. The presence of any urea containing byproducts will also be determined.
More information13C NMR Spectroscopy
13 C NMR Spectroscopy Introduction Nuclear magnetic resonance spectroscopy (NMR) is the most powerful tool available for structural determination. A nucleus with an odd number of protons, an odd number
More informationH H N - C - C 2 R. Three possible forms (not counting R group) depending on ph
Amino acids - 0 common amino acids there are others found naturally but much less frequently - Common structure for amino acid - C, -N, and functional groups all attached to the alpha carbon N - C - C
More informationError Tolerant Searching of Uninterpreted MS/MS Data
Error Tolerant Searching of Uninterpreted MS/MS Data 1 In any search of a large LC-MS/MS dataset 2 There are always a number of spectra which get poor scores, or even no match at all. 3 Sometimes, this
More informationMass Spectrometry Signal Calibration for Protein Quantitation
Cambridge Isotope Laboratories, Inc. www.isotope.com Proteomics Mass Spectrometry Signal Calibration for Protein Quantitation Michael J. MacCoss, PhD Associate Professor of Genome Sciences University of
More informationMass Frontier Version 7.0
Mass Frontier Version 7.0 User Guide XCALI-97349 Revision A February 2011 2011 Thermo Fisher Scientific Inc. All rights reserved. Mass Frontier, Mass Frontier Server Manager, Fragmentation Library, Spectral
More informationUsing Ontologies in Proteus for Modeling Data Mining Analysis of Proteomics Experiments
Using Ontologies in Proteus for Modeling Data Mining Analysis of Proteomics Experiments Mario Cannataro, Pietro Hiram Guzzi, Tommaso Mazza, and Pierangelo Veltri University Magna Græcia of Catanzaro, 88100
More informationXcalibur Versions 2.1.0 2.3.0
Thermo Xcalibur Versions 2.1.0 2.3.0 Qualitative Analysis User Guide XCALI-97211 Revision E August 2012 2012 Thermo Fisher Scientific Inc. All rights reserved. Xcalibur, Surveyor, and Accela are registered
More informationUsing Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract
Using Natural Products Application Solution with UNIFI for the Identification of Chemical Ingredients of Green Tea Extract Lirui Qiao, 1 Rob Lewis, 2 Alex Hooper, 2 James Morphet, 2 Xiaojie Tan, 1 Kate
More informationAgilent G2721AA/G2733AA Spectrum Mill MS Proteomics Workbench
Agilent G2721AA/G2733AA Spectrum Mill MS Proteomics Workbench Application Guide Agilent Technologies Notices Agilent Technologies, Inc. 2012 No part of this manual may be reproduced in any form or by any
More informationGuide to Reverse Phase SpinColumns Chromatography for Sample Prep
Guide to Reverse Phase SpinColumns Chromatography for Sample Prep www.harvardapparatus.com Contents Introduction...2-3 Modes of Separation...4-6 Spin Column Efficiency...7-8 Fast Protein Analysis...9 Specifications...10
More informationfor mass spectrometry calibration tools Thermo Scientific Pierce Controls and Standards for Mass Spectrometry
Thermo Scientific Pierce Controls and Standards for Mass Spectrometry calibration tools for mass spectrometry Ensure confidence in instrument performance with Thermo Scientific Pierce Calibration Solutions
More informationAccurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances
Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances TripleTOF 5600 + LC/MS/MS System with MasterView Software Adrian M. Taylor AB Sciex Concord, Ontario (Canada) Overview
More informationWATERS QUANTITATIVE ANALYSIS solutions
More sensitivity. More speed. What more can you ask for in a complete quantitative analysis solution? WATERS QUANTITATIVE ANALYSIS solutions THE CHALLENGE OF QUANTITATIVE ANALYSIS Tandem mass spectrometry,
More informationAxION edoor. Web-Based, Open-Access Mass Spectrometry Software
AxION edoor Web-Based, Open-Access Mass Spectrometry Software 2 AxION edoor is the open-access software solution for today s fast-paced laboratory. It is designed to operate and optimize the management
More informationOutline of MassHunter Software Session
Outline of MassHunter Software Session 1. Overview MassHunter Software Programs 2. From ChemStation to MassHunter (Optional) 3. MassHunter Qual and Quant (?) 4. MassHunter for LC/MS Demo Find by Methods:
More informationSimultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2
Technical ote Simultaneous Metabolite Identification and Quantitation with UV Data Integration Using LightSight Software Version 2.2 Alek. Dooley, Carmai Seto, esham Ghobarah, and Elliott B. Jones verview:
More informationTutorial 9: SWATH data analysis in Skyline
Tutorial 9: SWATH data analysis in Skyline In this tutorial we will learn how to perform targeted post-acquisition analysis for protein identification and quantitation using a data-independent dataset
More informationQ-TOF User s Booklet. Enter your username and password to login. After you login, the data acquisition program will automatically start.
Q-TOF User s Booklet Enter your username and password to login. After you login, the data acquisition program will automatically start. ~ 1 ~ This data acquisition window will come up after the program
More informationA. A peptide with 12 amino acids has the following amino acid composition: 2 Met, 1 Tyr, 1 Trp, 2 Glu, 1 Lys, 1 Arg, 1 Thr, 1 Asn, 1 Ile, 1 Cys
Questions- Proteins & Enzymes A. A peptide with 12 amino acids has the following amino acid composition: 2 Met, 1 Tyr, 1 Trp, 2 Glu, 1 Lys, 1 Arg, 1 Thr, 1 Asn, 1 Ile, 1 Cys Reaction of the intact peptide
More informationLC-MS/MS for Chromatographers
LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS, with an emphasis on the analysis of drugs in biological matrices LC-MS/MS for Chromatographers An introduction to the use of LC-MS/MS,
More informationAgilent 6000 Series LC/MS Solutions CONFIDENCE IN QUANTITATIVE AND QUALITATIVE ANALYSIS
Agilent 6000 Series LC/MS Solutions CONFIDENCE IN QUANTITATIVE AND QUALITATIVE ANALYSIS AGILENT 6000 SERIES LC/MS SOLUTIONS UNRIVALED ACHIEVEMENT AT EVERY LEVEL The Agilent 6000 Series of LC/MS instruments
More informationApplication Note # MT-103 FAST-SRM: A new Single Reaction Monitoring Method for Fast, Targeted Drug Distribution Testing in Tissue Sections
ruker Daltonics pplication Note # MT-103 FST-SRM: new Single Reaction Monitoring Method for Fast, Targeted Drug Distribution Testing in Tissue Sections Targeted analysis of drug molecules and metabolites
More informationHigh-Throughput 3-D Chromatography Through Ion Exchange SPE
High-Throughput 3-D Chromatography Through Ion Exchange SPE Application Note 205 Luke Roenneburg and Alan Hamstra (Gilson, Inc.) Introduction 2-dimensional (2-D) separation is the separation of a sample
More informationChoices, choices, choices... Which sequence database? Which modifications? What mass tolerance?
Optimization 1 Choices, choices, choices... Which sequence database? Which modifications? What mass tolerance? Where to begin? 2 Sequence Databases Swiss-prot MSDB, NCBI nr dbest Species specific ORFS
More informationOverview. Introduction. AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS System
Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS
More informationIdentification of Serum Protein Biomarkers for Autistic Spectrum Disorder. Melissa Butkiewicz
Identification of Serum Protein Biomarkers for Autistic Spectrum Disorder by Melissa Butkiewicz Clarkson University Identification of Serum Protein Biomarkers for Autistic Spectrum Disorder A Thesis Proposal
More informationProtein Hit1, a novel box C/D snornp assembly factor, controls cellular concentration of protein Rsa1p by direct interaction
Supplementary data Protein Hit1, a novel box C/D snornp assembly factor, controls cellular concentration of protein Rsa1p by direct interaction Benjamin Rothé, Jean-Michel Saliou, Marc Quinternet, Régis
More informationAppNote 6/2003. Analysis of Flavors using a Mass Spectral Based Chemical Sensor KEYWORDS ABSTRACT
AppNote 6/2003 Analysis of Flavors using a Mass Spectral Based Chemical Sensor Vanessa R. Kinton Gerstel, Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USA Kevin L. Goodner USDA, Citrus & Subtropical
More informationLC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research
LC-MS/MS Method for the Determination of Docetaxel in Human Serum for Clinical Research J. Jones, J. Denbigh, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20581 Key Words SPE, SOLA,
More informationflexanalysis User Manual
flexanalysis User Manual Version 3.0 (September 2006) Copyright Copyright 2006 Bruker Daltonik GmbH This document is published by Bruker Daltonik Bremen. All rights reserved. Reproduction, adaptation or
More informationaurora M90 Innovation with Integrity A new era in ICP-MS ICP-MS
aurora M90 A new era in ICP-MS Innovation with Integrity ICP-MS Your Partner in ICP-MS Solutions Bruker continues to find new and novel ways to meet your changing needs. As a leader in elemental analysis
More informationIntroduction to mass spectrometry (MS) based proteomics and metabolomics
Introduction to mass spectrometry (MS) based proteomics and metabolomics Tianwei Yu Department of Biostatistics and Bioinformatics Rollins School of Public Health Emory University September 10, 2015 Background
More informationChapter 3. Protein Structure and Function
Chapter 3 Protein Structure and Function Broad functional classes So Proteins have structure and function... Fine! -Why do we care to know more???? Understanding functional architechture gives us POWER
More informationPeptidomicsDB: a new platform for sharing MS/MS data.
PeptidomicsDB: a new platform for sharing MS/MS data. Federica Viti, Ivan Merelli, Dario Di Silvestre, Pietro Brunetti, Luciano Milanesi, Pierluigi Mauri NETTAB2010 Napoli, 01/12/2010 Mass Spectrometry
More informationWaters Core Chromatography Training (2 Days)
2015 Page 2 Waters Core Chromatography Training (2 Days) The learning objective of this two day course is to teach the user core chromatography, system and software fundamentals in an outcomes based approach.
More informationIndustry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development
Industry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development Dawn Stickle, Agilent Technologies Originally presented by Eric Fang, Novartis Overview
More information