EMBO WORKSHOP 2005 CHROMOSOME STRUCTURAL ELEMENTS: FROM DNA SEQUENCE TO FUNCTION

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1 EMBO WORKSHOP 2005 CHROMOSOME STRUCTURAL ELEMENTS: FROM DNA SEQUENCE TO FUNCTION Villa Mondragone, Monte Porzio Catone, Rome, Italy September 29-October 3, 2005 Organizer: Fiorentina Ascenzioni, Dipartimento di Biologia Cellulare e dello Sviluppo, University of Rome La Sapienza, via dei Sardi 70, Rome, Italy Co-organizers: - Silvia Bacchetti, Istituto Regina Elena, via delle Messi d Oro, 156, Rome, Italy - Giuseppe Novelli, Dipartimento di Biopatologia e Diagnostica, University of Rome Tor Vergata, via Montepellier 1, Rome, Italy - Maria Savino, Dipartimento di Genetica e Biologia Molecolare, University of Rome La Sapienza, P.le Aldo Moro 5, Rome, Italy Person to whom enquiries may be addressed: Fiorentina Ascenzioni, 1

2 Workshop Programme Talks (T) 25 min + 5 min discussion; Short Talks (ST): 12 min + 3 min discussion, P, Posters 29/09/2005 Thursday 4-6 pm: Registration, hanging of posters 6 pm: Welcoming remarks: Silvia Bacchetti/Fiorentina Ascenzioni 6:15-7:15 pm: Lecture: Lucio Luzzatto (IT), Chromosomal and molecular basis of cancer. Cocktails 30/09/2005 Friday am Session 1: Telomeres: structure, proteins and positioning Chair: P. Donini (IT) 9:00 T1- Patrick Kaminker (USA), Dual function for a telomere-associated protein? 9:30 T2- Daniela Rhodes (UK), Towards the structure of the 30 nm chromatin fiber. 10:00 ST1- Heico Schoiber (CH), Telomerase Helps Tether Telomeric Chromatin to the Nuclear Periphery via Interaction with yku am Coffee break 11 am pm 11:00 T3- Stefano Cacchione (IT), The role of DNA sequence in telomeric chromatin features 11:30 T4- Hans Lipps (DE), Telomere end-binding proteins control the formation of G- quadruplex DNA structures in vivo. 12:00 ST2- K. Lemke (Poland). Interaction of antitumor triazoloacridone C-1305 with guaninerich DNA: induction of structural changes in guanine triplets and stabilization of G-quadruplexes 12:15 ST3- H. Renauld (USA), Interstitial (sub)telomeric repeats: Punctuation marks of genome dynamics? pm Lunch pm Session 2: Telomere replication and telomere length regulation Chair: Virginia Zakian (USA) 2:00 T5- Virginia Zakian (USA), Pif1p family helicases: effects on telomerase. 2:30 T6- Eric Gilson (FR), New pathways of telomere length regulation in yeast. 3:00 T7- Arturo Londoňo-Vallejo (FR), Telomere homeostasis in humans: length control at cell and single telomere levels. 3:30 ST4- S. Bekaert (Belgium), Reference values and basic determinants of telomere length in a large middle-aged population. 3:45 ST5- D. Baird (UK), Telomere dynamics and instability. 4:00 ST6- M.A. Cerone (Canada) A human cell line that maintains telomeres in the absence of telomerase and of key markers of ALT. 2

3 4.15 ST7- C.M. Counter (USA) Loss of hpot1 function leads to telomere instability and a cutlike phenotype pm Coffee break 5:00-7:15pm Session 3: Chromosome replication and disjunction Chair: Hans Lipps (DE) 5:00 T8- Michelle Debatisse (FR), Dynamics of DNA replication in mammalian somatic cells 5:30 T9- Silvano Riva (IT), Functional analysis of a human DNA replicator. 6:00 T10- Susan Smith (USA), Telomeres require special mechanisms for their compaction and resolution at mitosis. 6:30 ST8- Peter Meister (CH), Spatial and temporal separation of replication and recombination requires the intra-s checkpoint 6:45 ST9- M. Cornfort (USA), Excess of Radiation-Induced Dicentrics Involving Homologous Chromosomes. 7:00 ST10- B. Meier (USA), The C. elegans catalytic subunit of telomerase TRT-1 and DNA damage signaling ST11- S. Bailey (USA), Telomeres and Double-Strand Breaks: Tying up Loose Ends. 01/10/2005 Saturday am Session 4: Centromeres, DNA sequences and chromatin plasticity Chair: Brenda Grimes (USA) 9:00 T11- Beth A. Sullivan (USA), Centromeric Chromatin is a Continuous, Dynamic Domain that Permits Gene Expression 9:30 T12- Christine Farr (UK), Topoisomerase II: untangling its contribution at centromere 10:00 T13- Giuliano Della Valle (IT), Dissecting Cenp-C function in mammalian centromeres am Coffee break 11.00am pm Session 4 continued 11:00 T14- W.C. Earnshaw (UK), Genetic analysis of chromosome segregation in vertebrate cells. 11:30 ST12- C. Desmaze (FR), Chromatin structure and dynamics in the formation of chromosome aberrations. 11:45 ST13- C. Woo (USA), Long-range Nucleosome Mapping of the HOX Gene Cluster. 12:00 ST14- J. Fajkus (CZ), Minisatellite telomeres in the family Alliacae are lost with divergence of the genus allium pm Poster session with Lunch 3.30pm 6pm 7:30pm Departure for Rome by turist coach Guided visit to part of the Musei Capitolini and the Campidoglio (City Hall of Rome) Cocktails and dinner on the adjoining Terrazza Caffarelli 3

4 02/10/05 Sunday 9-10:30am Session 5: De novo centromeres assembly and Chromosome Engineering Chair: W.C. Earnshaw (UK) 9:00 T15- Brenda Grimes (USA), Segregation of human artificial chromosomes in mouse cells. 9:30 T16- T. Voet, (Belgium), Optimisation and exploitation of a chromosomal vector 10:00 ST15- N. Mestrovic (Croatia), The MEL-172 satellite DNA library in parthenogenetic meloidogyne species: conserved pattern of nucleotide variability along the monomers am Coffee break 11.00am pm Session 5 continued Chair: Christine Farr (UK) 11:30 T17- H. Masumoto (JPN), Chromatin assemblies required for de novo human artificial chromosome formation. 12:00 T18- Fiorentina Ascenzioni (IT), Human minichromosomes: expression vectors for human genes pm Poster session with Lunch pm Lecture: Giorgio Bernardi (IT), Chromatin structure in interphase nuclei of vertebrates and the formation GC-rich isochores. 4: pm Coffee break :00pm session 6: Chromosomes organization and gene evolution Chair: Giuseppe Novelli (IT) 4:30 T19- M. Cremer (DE), Nuclear architecture in the light of conserved higher order chromatin arrangements. 5:00 T20- Mariano Rocchi (IT), Centromere repositioning in evolution. 5:30 T21- Novelli Giuseppe (IT), The role of the nuclear envelope in genome organisation. Closing remarks: Silvia Bacchetti and Giuseppe Novelli 2/ or 3/10/05 Departure 4

5 Posters* P1 - C. Auriche (IT), Chronic Chromosome Instability in Humanized Yeast P2 - C. Azzalin (CH), The human RNA surveillance factors UPF1 and EST1A/SMG6 are essential for S-phase progression and DNA repair. P3 - M. Babar (Pakistan), Identification of bacterial blight resistance genes Xa4 in Pakistani rice germplasm using PCR based markers. P4 - S. Carrabino (IT), Polyethylenamine/albumin complexes display high transfection efficiency in confluent and polarized human respiratory epithelial cells. P5 - I. Draskovic (FR), Molecular mechanisms of alternative lengthening of telomeres (ALT) in human cancers. P6 A. Farsetti (IT), Primary cultures of prostate luminal cells as a model for an integrated approach to prostate cancer: isolation, characterization and gene profiling. P7 - M. Gezahagne (Ethiopia), Species distinctiveness between Trifolium quartinianum and Trifolium steudneri. P8- C. Keil (DE), Functional interactions of poly(adp-ribose)glycohydrolase with PARP-1 and XRCC1 P9- C. Lan (Taiwan), Significance of Topoisomerase 2a in association with Her2/neu in breast cancer management. P10- S-C. Liang (Taiwan), Chromosomal aberrations in malignant pleural effusion from lung adenocarcinoma: varying cytogenetic abnormalities detected in relationship to gender and smoking. P11 - L-C. Lin (Taiwan), EGFR gene sequence in breast cancer patients in Taiwan. P12 C. Mondello (IT), Cellular transformation of telomerase immortalized human fibroblasts. P13 B. Mravinac (Croatia), What a difference junk DNAs make.. P14 V. Petrovic (Croatia), Intricate patterns of sequence divergence and conservation in an organizationally complex Donax trunculus satellite DNA family. P15 G. Piaggio (IT), NF-Y dependent chromatin remodelling in proliferation and differentiation. P16 S. Redon (FR), Protein-protein and protein-rna interactions mediate the association of human EST1a with telomerase. P17 E. Sykorova (CZ), Plant telomerases synthesizing human type of telomeres. * Poster will be displayed for the duration of the meeting. Each poster has been assigned a number according to the list above. Presenting authors are requested to stand alongside their poster during the poster sessions. Maximum size of the poster: 1.20cm high x 1.50 width cm 5

6 Travelling to Villa Mondragone From Leonardo da Vinci-Fiumicino Airport 1. Take the Express Train (Leonardo Express) to Rome Termini Station (about E8). The train leaves every 30 min (7 and 37 min after the hour) and takes about 30 min to reach the Termini Station. 2. Take the local train to Frascati. Tickets for this train can be bought at the Airport Station. The train for Frascati leaves every hour beginning at 5:46am. Following trains are at: 6:32am / 7:33am / 8:35am and then 52 min after the hour till 21:52pm. 3. At the Frascati Station take a taxi to Villa Mondragone or Hotel Villa Vecchia. Notification of where you will be staying will be posted on the site at a later time. 4. Alternatively, take a taxi from the airport to Villa Mondragone or Villa vecchia (about E60). Please make sure to discuss the fare at departure. From Ciampino Airport 1. Take the shuttle bus (leaving every 20 min till 23:30pm) or a taxi to Ciampino Train Station. From there take the train to Frascati which leaves at: 4:49am / 6:02am / 6:50am/ 7:47am / 8.53am / 10:08am and then at 8 min after the hour till 22:08pm. 2. From Frascati Station take a taxi to Villa Mondragone or Hotel Villa Vecchia. Notification of where you will be staying will be posted on the site at a later time. 3. Alternatively take a taxi from Ciampino Airport to Villa Mondragone or Villa vecchia (about E25). Please make sure to discuss the fare at departure. Local Taxi companies Taxi Frascati: 06/ Alfredo Iacoangeli: Via G. Saragat, Genzano (RM) 328/ / Andrea Amore: 338/ , 328/ , 340/ Migliori Gianni: 349/

7 Venue Villa Mondragone Via Frascati 51, 0040 Monteporzio Catone, Rome Tel Accomodation Hotel Villa Vecchia, via Frascati 49, 0040 Monteporzio Catone, Rome tel , fax, Scientific Session All lectures will be held in Sala degli Svizzeri Poster will be displayed in Sala Riunioni Meals and Refreshments Coffee breaks and lunches will be served in Villa Mondragone Sponsors: Università degli Studi di Roma La Sapienza Università degli Studi di Roma Tor Vergata Istituto Pasteur-Fondazione Cenci Bolognetti Ministero dell Istruzione, dell Università e della Ricerca Applied Biosystems 7

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