Single-Use Flow-Through Cell ph FTC-SU-HP8

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1 Single-Use Flow-Through Cell ph FTC-SU-HP8 SENSOR PROBES Instruction Manual

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3 Single-Use Flow-Through Cell ph FTC-SU-HP8 Specification: ph sensor stick integrated in a single-use flow-through cell Document filename: IM_FTC-SU-HP8_dv1 All rights reserved. No parts of this work may be reproduced in any form or by any means - graphic, electronic, or mechanical, including photocopying, recording, taping, or information storage and retrieval systems - without the written permission of the publisher. Products that are referred to in this document may be either trademarks and/or registered trademarks of the respective owners. The publisher and the author make no claim to these trademarks. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it. In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document. Specifications may change without prior notice. Manufacturer PreSens Precision Sensing GmbH Josef-Engert-Str Regensburg, Germany Phone Fax

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5 Table of Contents 1 Preface Description of the Single-Use Flow-Through Cell ph Scope of Delivery Measurement Set-up Operation Unpacking the FTC-SU-HP Assembling and Connecting the FTC-SU-HP Measurement Calibration Equilibration One point adjustment Technical Data Concluding Remarks... 10

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7 3 Preface 1 Preface You have chosen a new, innovative technology for measuring ph. Chemical optical sensors (also called optodes) have several important features: They are small. Their signal does not depend on the flow rate of the sample. They can be physically divided from the measuring system which allows a noninvasive measurement. They can be used in disposables. Therefore, they are ideally suited for the examination of small sample volumes, for highly parallelized measurements in disposables, and for biotechnological applications. A set of different minisensors, flow-through cells and integrated sensor systems is available to make sure you have the sensor which matches your application. Please feel free to contact our service team to find the best solution for your application. Your PreSens Team PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE WORKING WITH THIS ITEM.

8 4 Description of the Single-Use Flow-Through Cell ph 2 Description of the Single-Use Flow- Through Cell ph The single-use flow-through cell ph (FTC-SU-HP8) comprises a miniaturized chemical optical sensor integrated in a disposable flow-through cell. The sensor is fixed to a color coded stick (sensor stick = SST; red = ph), which can be attached to flow-through cells of different size and shape (via Luer connector), according to requirements. It is delivered with a disposable T-cell made of polycarbonate, which can be easily connected to external tubing via Luer-Lock adapters. A polymer optical fiber connects the sensor inside the flow-through cell to the respective measurement device (e. g. ph-1 mini). The single-use flow-through cells can be delivered beta-irradiated (FTC-SU-HP8-S) or untreated (FTC-SU-HP8-US). Fig. 1 Single-use flow-through cell ph (FTC-SU-HP8) 2.1 Scope of Delivery The FTC-SU-HP8 is double-packed. It is delivered in a transparent plastic bag (to enable unpacking of irradiated FTCs under sterile conditions) which is again packed in a lightproof packaging (to protect the sensor material). The FTC-SU-HP8 is pre-calibrated. Fig. 2 Packed single-use flow-through cell ph. The respective polymer optical fiber has to be ordered separately.

9 5 Description of the Single-Use Flow-Through Cell ph Additionally required equipment (not supplied): Polymer optical fiber (POF); with SMA connector on one side, available lengths 1.0, 1.5, and 5.0 m (for lengths of more than 5 m, please contact our service team) Fiber optic ph transmitter, e. g. ph-1 mini (more ph transmitters can be found on PC / Notebook If your application requires a T-cell with other inner diameter or of other design, you can integrate the sensor stick in another cell as well. The flow-through cell must have a Luer connector, so the sensor stick can be properly attached! Supplier for such cells compatible with the sensor stick is e. g. Qosina (Part Numbers in the 2014 catalogue: , ). 2.2 Measurement Set-up Fig. 3 Set-up for measurement with a FTC-SU-HP8 A polymer optical fiber, which is connected to a ph transmitter, is attached to the FTC-SU- HP8. The FTC-SU-HP8 is integrated e. g. in the flow path of a perfusion reactor or the bypass of a reactor.

10 6 Operation 3 Operation 3.1 Unpacking the FTC-SU-HP8 The FTC-SU-HP8 is delivered in a lightproof packaging to ensure a long shelf-life, so do not open this packaging immediately at delivery. It is recommended to unpack the FTC just before using it.! Take care when opening the lightproof packaging with scissors, especially when handling irradiated FTCs. Cut close to the welding seam of the packaging, not to cut into the inner plastic bag. Fig. 4 Opening the lightproof outer packaging. Inner packaging: FTC in transparent plastic bag.! If your FTC-SU-HP8 is delivered irradiated open the inner plastic bag with the FTC components in a laminar flow box, or in a similar controlled environment, and wear gloves! Fig. 5 FTC-SU-HP8: the sensor stick is attached to a disposable T-cell.

11 7 Operation 3.2 Assembling and Connecting the FTC-SU-HP8 If you want to use the sensor stick (SST) with another flow-through cell than the T-cell it is delivered with, you can simply remove it from the T-cell by turning it counter-clockwise at the Luer connector. Now you can attach the sensor stick to a flow-through cell of your choice. Make sure it is attached firmly, but do not use force! Fig. 6 Connecting the sensor stick (SST) to a flow-through cell. The FTC-SU-HP8 can be integrated in the flow path of your perfusion system. According to your tube size you can use end caps (Luer-Lock adapter). Attach the tubes at the inlet and outlet of the FTC. Fig. 7 Connecting the FTC with the tubes of the perfusion system (using Luer-Lock adapter). When the FTC is connected and the flow path is closed, the polymer optical fiber can be attached. (Do not attach the polymer optical fiber before closing the flow path, if you want to avoid contamination; the fiber is not irradiated.) First loosen the red cap on the sensor stick. Then insert the polymer optical fiber in the sensor stick until stop, and fasten it with the red cap by turning the cap clockwise.

12 8 Operation Fig. 8 Loosen the red cap and insert the polymer optical fiber until stop; then fasten the red cap again. Connect the polymer optical fiber to the SMA connector on the front panel of your ph transmitter.! As the sensor stick and the flow-through cell are not glued together, please check the connection before using the FTC, and tighten it again if necessary, but without using force! Fig. 9 Tighten the connections before use! Medium can be pumped through the FTC-SU-HP8 now.

13 9 Operation 3.3 Measurement Calibration The FTC-SU-HP8 is delivered pre-calibrated. The enclosed Final Inspection Protocol contains the calibration values, which have to be entered into the software of the fiber optic ph transmitter you are using. (Please see the respective transmitter instruction manual for more detailed information on calibration.) In case of samples differing significantly from physiological buffers a multi-point calibration is recommended. Prepare at least five solutions, of similar composition like the sample and different ph, covering the range of interest. Place the sensor stick in the vessels containing the solutions of known ph or pump the solutions through the FTC one after another - and perform the multi-point calibration like described in the respective transmitter instruction manual. If the vessel is large enough to place a ph electrode, we recommend titrating directly in the vessel and following the instructions for multi-point calibration in the transmitter manual Equilibration! The sensor needs to be equilibrated before use. In order to do so you have to fill the FTC with your medium and wait for at least 15 minutes so that the sensor can equilibrate One point adjustment ph one point adjustment is advised if your media differs from physiological conditions to obtain optimal sensor performance. Ideally the starting ph of the sample is known. If the phase value is constant, follow the instructions for auto zero in the transmitter manual.

14 10 Technical Data / Concluding Remarks 4 Technical Data Specifications* Measuring range ph Response time (t90) at 37 C** < 120 sec. Resolution at ph = 7 ± 0.02 ph Accuracy at ph = 7 ± 0.05 after single point calibration at ph 7 Drift at ph = 7: < ph per day (sampling interval of 1 min.) Measurement Temperature From 5 C to 50 C Properties* Compatibility Aqueous solutions, ethanol (max. 10 % V/V), methanol (max. 10 % V/V), ph 2-10 Cross Sensitivity Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere Sterilization procedure*** Irradiation, Ethylene oxide (EtO) Calibration SST are pre-calibrated; Single point calibration is recommended Storage Stability 18 months provided the sensor is stored in the dark *provided ph sensor is used without further handling in physiological solutions **equilibrated FTC with physiological solution and sufficient flow rate (min. 10 ml/min) at 37 C ***recalibration may be required 5 Concluding Remarks Dear Customer, With this manual, we hope to provide you with an introduction to work with the single-use flow-through cell ph (FTC-SU-HP8). This manual does not claim to be complete. We are endeavored to improve and supplement this version. We are looking forward to your critical review and to any suggestions you may have. You can find the latest version at With best regards, Your PreSens Team

15 11 Technical Data / Concluding Remarks Manufacturer PreSens Precision Sensing GmbH Josef-Engert-Straße Regensburg Germany Phone Fax

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