Prof. Susanna Dolci M.D., Ph.D. born 6 December 1960 Current position: Associate Professor of Anatomy, University of Rome Torvergata, Italy

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1 Prof. Susanna Dolci Curriculum Vitae Prof. Susanna Dolci M.D., Ph.D. born 6 December 1960 Current position: Associate Professor of Anatomy, University of Rome Torvergata, Italy Education and academic career 1985 MD, University of Rome La Sapienza, Italy 1991 PhD in Cell Biology, University of Rome La Sapienza 1993 Assistant Professor of Anatomy at the University of Rome Torvergata, Italy Associate Professor of Anatomy at the University of Rome Torvergata, Italy Fellowships research visits Visiting Scientist Mammalian Genetics Laboratory, NCI, Frederick MD, USA 1998 Research Associate The Wellcome Trust, Cambridge, UK Reviewer for Research Foundations/Organisations Swedish Ministry of Education and Research (SW) Italian Ministry of University and Scientific Research (IT) Reviewer for International Journals Development J. Cell Science Oncogene FUNDING CNR-NATO grant FIRB PRIN PRIN PRIN PUBLICATIONS H-Index: 25 Total IF: 247 Total publications 62 Total citations 2885 Citation mean 60 Top 10 original publications as senior author ) Developmental expression of BMP4/ALK3/SMAD5 signaling pathway in the mouse testis: a potential role of BMP4 in spermatogonia differentiation. Pellegrini M, Grimaldi P, Rossi P, Geremia R, Dolci S. J Cell Sci. 2003; 116: IF:6,290 2) Imatinib mesylate inhibits Leydig cell tumor growth: evidence for in vitro and in vivo activity. Basciani S, Brama M, Mariani S, De Luca G, Arizzi M, Vesci L, Pisano C, Dolci S, Spera G, Gnessi L. Cancer Res. 2005; 65: IF: 8,234 3) Repression of kit expression by Plzf in germ cells. 1

2 Filipponi D, Hobbs RM, Ottolenghi S, Rossi P, Jannini EA, Pandolfi PP, Dolci S. Mol Cell Biol. 2007; 27: IF: 6,188 4) Increased expression and nuclear localization of the centrosomal kinase Nek2 in human testicular seminomas. Barbagallo F, Paronetto MP, Franco R, Chieffi P, Dolci S, Fry AM, Geremia R, Sette C. J Pathol. 2009; 217: IF: 7,271 5) ATRA and KL promote differentiation toward the meiotic program of male germ cells. Pellegrini M, Filipponi D, Gori M, Barrios F, Lolicato F, Grimaldi P, Rossi P, Jannini EA, Geremia R, Dolci S. Cell Cycle. 2008; 7: IF: 4,999 6) Platelet-derived growth factor receptor beta-subtype regulates proliferation and migration of gonocytes. Basciani S, De Luca G, Dolci S, Brama M, Arizzi M, Mariani S, Rosano G, Spera G, Gnessi L. Endocrinology. 2008; 149: IF:4,992 7) Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells.barrios F, Filipponi D, Pellegrini M, Paronetto MP, Di Siena S, Geremia R, Rossi P, De Felici M, Jannini EA, Dolci S.J Cell Sci. 2010;123: IF: 6,290 8) Targeted JAM-C deletion in germ cells by Spo11-controlled Cre recombinase. Pellegrini M, Claps G, Orlova VV, Barrios F, Dolci S, Geremia R, Rossi P, Rossi G, Arnold B, Chavakis T, Feigenbaum L, Sharan SK, Nussenzweig A. J Cell Sci. 2011,124: IF: 6,290 9) RanBPM is essential for mouse spermatogenesis and oogenesis.puverel S, Barrick C, Dolci S, Coppola V, Tessarollo L. Development. 2011:138: IF: 6,898 10) Barrios F, Filipponi D, Campolo F, e Gori M, Bramucci F, Pellegrini M, Ottolenghi S, Rossi P, Jannini EA, Dolci S. SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development. J Cell Sci. 2012; in press IF: 6,290 IMPEGNO DIDATTICO-FORMATIVO DEL DOCENTE NELL AMBITO DEL DOTTORATO A) Tutor durante l attività sperimentale, nell ultimo anno accademico e/o precedenti, dei seguenti dottorandi: nome e cognome del dottorando Federica Campolo Florencia Barrios Manuele Gori Doria Filipponi B) Tutor durante la preparazione della tesi di dottorato, nell ultimo anno accademico e/o precedenti, dei seguenti dottorandi: nome e cognome del dottorando Federica Campolo Florencia Barrios Manuele Gori Doria Filipponi. C) Attività seminariale, nell ultimo anno accademico e/o precedenti: Titolo seminario: 1) Sox2 positive Adult Stem and Progenitor Cells are important for Tissue regeneration 2

3 and Survival of mice 2) FGF9 suppresses meiosis and promotes male germ cell fate 3) Spermatogonial stem cells:role of PLZF in the control of germ cell stemness 4) The fate of postnatal male germ cells. Stemness or not stemness.a brief history on how spermatogonia decide of their fate 5) Sohlh1 and 2 control Kit expression in postnatal spermatogonia 3

4 Research interests: 1)Embryonic and postnatal development of germ cells and sex determination. We focused primarily onto the understanding of the developmental potential of germ cells either into the mitotic and into the meiotic pathways. In particular we studied the mechanisms underlying the in vitro proliferation ability of primordial germ cells (PGCs) and spermatogonia, identifying two growth factors (SCF and LIF) able to support PGCs survival and spermatogonia proliferation (SCF). We characterized the signal transduction pathway activated by SCF in mitotic spermatogonia and identified the telomerase gene as one of the molecular targets of Kit activation. We described a novel transduction pathway mediated by BMP4 in postnatal spermatogonia and in PGCs. We recently identified the molecular mechanisms of germ stem cell differentiation, by showing that the transcriptional repressor PLZF directly represses c-kit expression. We also found that retinoic acid can promote meiotic entry in vitro of male mitotic postnatal germ cells We studied the genomic organization of the sex determining gene Sry, its expression in the male fetal gonads and in meiotic and post meiotic male germ cells, and its co-expression with the Xist gene, which is involved in X-chromosome inactivation. In these studies, a potential promoter of the precursor of the circular form of Sry was identified, which is active both in the fetal gonads and in the male germ cells. 2)Meiotic differentiation of male germ cells. Our group identified FGF9 and Retinoic Acid as the factors which regulate meiosis in the fetal and postnatal male gonads. 3) Regulation of Kit expression in germ cells. We identified the transcription factor(s) involved in Kit expression in postnatal male germ cells. We produced transgenic mice overexpressing the Kit gene under its own promoter (BAC transgenesis). The Kit gene was mutated in the 814 residue to produce a dominant active protein to promote neoplastic transformation of germ cells. These animals do not express the transgene in all the target tissues, suggesting that the BAC utilized in this study does not bear all the c-kit regulatory regions. 4)Sox2 requirement for germ cell development. We studied the role of Sox2 in the determination of the germ cell lineage by conditional gene targeting. We identify Sox2 as one of the first genes required in the hierarchy of germ cell differentiation. Interdisciplinary collaborations: Prof. S. DiStasi (University of Rome Torvergata). Control of Mitomycin treatment of bladder cancers. Prof. E A. Jannini (University of L Aquila). Development of corpora cavernosa of the penis in animal models. Dr. L. Tessarollo (NCI, Frederick, USA). Mouse transgenic models. Jesus Del Mazo (Centro de Investigaciones Biológicas, Madrid). Reproductive toxicology. 4

5 Componenti dell Unità: Prof. Susanna Dolci (prof. ass.) Dr.ssa Donatella Farini (ricercatore) Dr.ssa Valentina Montanari (dottoranda) 5