Laboratory Safety Inspection Checklist (Biology and Biochemistry)

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1 EOSMS 201-1A Date: 11/15/14 Page 1 of 9 Building Room Inspection Date Department Principal Investigator(s) Inspector(s) Instructions: This form is to be completed for laboratory self-inspection. Complete the following inspection form; one for each laboratory space. Answer each question as follows: o Yes (Requirement/criteria for item has been met) o No (Requirement/criteria for item has not been met) o CDI (Item was corrected during the inspection) o NA (Item is not applicable to the lab) For each item marked No, document the issue, the best corrective action, and the responsible party (lab, facilities, EHS, etc.) Keep the completed inspection form for your records Scan and copy of each completed inspection form to 1. Training and Documentation Are lab personnel current with all required safety training (EHS, departmental, lab specific)? 2. Are records of completed training maintained? Are written standard operating procedures (SOPs) available for working with biohazards? Are written SOPs available working with special chemical hazards (e.g. carcinogens, pyrophorics, explosives, acutely toxic materials, etc.)? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 1

2 EOSMS 201-1A Date: 11/15/14 Page 2 of Are lab personnel familiar with safety data sheets (SDSs) and how to access them? General a. Housekeeping Does it appear that the lab is free of food and drinks (and empty containers)? 7. Is a sink available for hand washing? 8. Are soap and paper towels available? 9. Are empty chemical bottles disposed of properly (triple rinsed, defaced, etc)? 10. Is equipment clean? 11. Does equipment appear to be in working condition? 12. Are tripping hazards taped to the floor (electrical cords, hoses, computer cables)? 13. Are spills (which can be mitigated safely) cleaned up immediately? 14. Are glass items stored appropriately on shelves or in cabinets? 15. Is an adequately stocked spill kit available? 16. Is a first aid kit available? 17. Is the first aid kit adequately stocked? 18. Is the lab free of excess clutter (e.g. empty boxes, empty chemical containers, broken equipment, etc.)? 19. Does it appear that lighting is adequate and functioning properly? 20. Is the lab free of unusual odors, unusual conditions, and/or visible airborne contaminants? 21. Are general waste receptacles emptied regularly? Warning a. Signs / Labels (RTK HazCom Act) EOSMS 201-1A Laboratory Safety Inspection Checklist Page 2

3 EOSMS 201-1A Date: 11/15/14 Page 3 of Are all appropriate warning signs posted on the door (biohazard, radioactive, flammable, corrosive, etc.)? 23. Is the emergency contact information posted on the door? 24. Is the PI/Professor/Lab manager identified in the contact information? 25. Is the emergency contact information up-to-date? 26. Are secondary chemical (working) containers labeled as to their contents (i.e. the full chemical name; not an abbreviation or chemical formula)? 27. Are labels in good condition (not deteriorated and/or illegible)? Is all equipment used with biologically hazardous labeled with a biohazard symbol? Are designated areas where particularly hazardous substances (PHSs) are used properly marked? 30. Are microwaves labeled Lab Use Only? 31. Are household refrigerators labeled Not safe for Flammable Storage? 32. Are all refrigerators and freezers labeled No Food or Drinks or with similar verbiage? Chemical a. Storage (NFPA Code, RTK HazCom Act) 33. Are chemicals stored appropriately on shelves (i.e. not on the floor)? 34. Are chemicals stored in cabinets rather than on the work bench? 35. Are liquid chemicals stored below eye level? (solids stored above liquids) 36. Are chemical containers in good condition (no rust, label readable, etc.)? Are chemicals segregated according compatibility (i.e. acids separate from bases, oxidizers separate from flammables, etc.)? Are chemicals segregated according to hazard class (corrosive, toxic, reactive, flammable, etc.)? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 3

4 EOSMS 201-1A Date: 11/15/14 Page 4 of Are liquid corrosives stored in secondary containment? 40. Is perchloric acid stored separately from organic materials and other acids? 41. Are chemicals that have a shelf life labeled with the date opened? 42. Are chemicals entered into the chemical inventory system (Chematix)? 43. Are chemicals labeled with the Chematix inventory label? 44. Are flammable liquids stored in a flammable liquid storage cabinet? Are flammable materials (which must be stored at low temperatures) stored in an explosion proof refrigerator? Is the volume of stored flammable materials within the maximum allowable quantity (per NFPA 45)? 47. Is picric acid wetted to prevent possible shock explosion? 48. Are water-reactive compounds properly stored (immersed in appropriate solutions, etc.)? 49. Are air-reactive compounds properly stored (in dessicant, etc.) 50. Are potentially explosive chemicals stored appropriately? 51. Are compressed gas cylinders properly secured (i.e. - stored upright and chained/strapped to a sturdy, stationary surface)? 52. Are compressed gases (which are not in use) labeled as empty or full? 53. Are compressed gases stored by compatibility groups? 54. Do compressed gas cylinders have the cap in place when not in use? 55. Are lecture bottles in good condition and stored appropriately (no rust; labeled, and stored upright in an appropriate rack)? 56. Are alternatives to mercury thermometers used when possible? 57. Are all mercury thermometers in good condition (i.e.- not leaking or broken)? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 4

5 Environmental Health & Safety EOSMS 201-1A Date: 11/15/14 Page 5 of 9 Biosafety a. (CDC, NIH) Are procedures which involve the manipulation of infectious agents and may potentially create aerosols conducted in a biosafety cabinet or other containment device? Are mechanical pipetting devices used when working with infectious materials (i.e. no mouth pipetting)? 60. Are centrifuge doors equipped with interlocking mechanisms? When centrifuging biohazardous samples, is secondary containment used (e.g. sealed rotors, centrifuge safety caps, etc.)? Are lab furniture, floors, and surfaces made of non-porous materials that can be decontaminated easily? Are work surfaces decontaminated with the appropriate disinfectant after working with infectious materials (e.g. 10 percent bleach, 70 percent ethanol)? 64. Are operable windows that open to the outside fitted with screens? 65. Are doors self-closing and lockable? Do suction flasks used for collection of biohazard waste contain liquid disinfectant (e.g. bleach solution)? Is the lab free of all animals that are not associated with laboratory work (excluding service animals)? Waste a. Disposal (EPS/GAEPD Regulations) 68. Are waste containers labeled as to their contents (e.g. chemical name; methanol waste; biological hazard; human cell culture waste)? 69. Are waste containers closed unless adding waste? 70. Are appropriate containers used for the collection of hazardous waste? 71. Are waste containers in good condition (no old containers, defaced)? 72. Are sharps collected in a proper collection box (hard-walled sharps container) before being offered for disposal? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 5

6 EOSMS 201-1A Date: 11/15/14 Page 6 of Are reusable sharps stored appropriately (i.e. sharp edges are not left exposed)? Is all broken glass placed in an appropriate container before being offered for disposal? Does it appear that all biohazard waste is autoclaved or chemically treated before being offered for disposal? Is the autoclave validated using the spore test method prior to autoclaving waste? 77. Is the log of autoclaved biohazard waste up-to-date? 78. Is chart or printer paper used to record each autoclave load being replaced regularly? 79. Has bagged waste been removed from the autoclave area? Have all biohazard symbols on bags been covered with tape that produces the word Autoclaved? Are unautoclaved biohazard bags stored in appropriate secondary containment prior to autoclaving? Chemical a. Fume Hoods/Biological Safety Cabinets 82. Have fume hoods been inspected/certified within one year? 83. Are fume hoods clear of clutter? 84. Are fume hoods sashes at 18 or below? 85. Are fume hood sashes intact, not cracked or broken? 86. Are fume hoods clear of electrical power strips? 87. Are all chemical containers inside the fume hood closed to prevent evaporation? 88. Are containers inside the fume hood stored in secondary containment? 89. Are work surfaces in the fume hood clean? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 6

7 EOSMS 201-1A Date: 11/15/14 Page 7 of Is fume hood baffle system free of obstructions? 91. Is fume hood free of loose paper? 92. Are water/air/gas valves on the front of the fume hood free of hanging items? 93. Have biological safety cabinets been inspected/certified within one year? 94. Are biological safety cabinets clear of clutter? 95. Is biological safety cabinet free of loose paper? 96. Are grills on the biological safety cabinet free of obstructions? 97. Is the top of the biosafety cabinet free of storage? 98. Is the biosafety cabinet free of Bunsen burners and an acceptable alternative is used for sterilization (e.g. electrical incinerator/sterilizer)? 99. Personal a. Protective Equipment Is personal protective equipment (i.e. lab coats, gloves, safety glasses/goggles) available to lab personnel? 100. Do lab personnel wear appropriate PPE when working in the lab? Do lab personnel wear appropriate attire to the lab (long pants/skirts, shoes that cover the whole foot)? Are gloves to be worn in the lab selected based on specific tasks (e.g. thermal gloves are used in procedures involving extreme temperatures)? 103. Is contaminated PPE disposed of in the appropriate waste stream(s)? 104. Do lab personnel remove all PPE before exiting the lab? Do lab personnel wash their hands after removing their PPE, and before leaving the lab? Have employees who are required to wear respirators been trained, medically evaluated, and fit tested for the respirator they wear? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 7

8 EOSMS 201-1A Date: 11/15/14 Page 8 of Have employees who wear respirators voluntarily been provided with Appendix D of (Information for Employees Using Respirators When Not Required Under the Standard) and signed the acknowledgement? Do employees wear hearing protection for processes that have a high level of noise? Emergency Procedures/Emergency Eyewash / a. Showers (ANSI Standards, RTK HazCom Act) 109. Do lab personnel know how to report accidents/near misses? 110. Are lab personnel comfortable cleaning up/containing small spills? 111. Are lab personnel familiar with emergency notifications and evacuation procedures? 112. Are all eyewash stations clear of obstructions? 113. Are all emergency shower stations clear of obstructions? 114. Are emergency showers available within 100 feet of the hazardous work area? 115. Are eyewashes being flushed on a weekly basis (check tag)? 116. Are eyewash caps kept in place when the eyewash is not in use? 117. Are emergency shower checks up to date (within one year)? 118. Is the solution in sterile eyewash squeeze bottles in good condition (check expiration date)? Fire/Life Safety (NFPA Code) 119. Are lab doors kept closed when not in use? 120. Is the direction of airflow in the lab negative to the hallway/corridor? 121. Are fire extinguishers visible and accessible? 122. Are walkways clear (48 inches unobstructed for main egress pathway)? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 8

9 EOSMS 201-1A Date: 11/15/14 Page 9 of Are exit doors unobstructed? 124. Are doors that do not lead to an exit labeled Not an Exit? 125. Are emergency exits clearly marked and well illuminated? 126. Are emergency evacuation routes clearly posted in the work area (e.g. in the hallway or near elevators)? 127. Are fire extinguishers being checked monthly? 128. Is Bunsen burner tubing in good shape (flexible)? 129. Are items stored more than 18 below the ceiling? 130. Are all ceiling tiles in place? Electrical a. Safety 131. Does permanent equipment have permanent wiring (no extension cords)? Are electrical cords/outlets/plugs in good condition (not pinched, cracked, frayed, cut, or burned)? Are electrical cords/outlets/plugs in good condition (not pinched, cracked, or burned)? 134. Are electrical power strips (with fuse) used rather than 3-way plugs? Are electrical outlets within reach of water equipped with a ground fault circuit interrupter? Are extension cords on temporary equipment visible with no defects (frays, cuts, splicing, abrasions, etc.)? Are extension cords on temporary equipment visible with no defects (cuts, abrasions, etc.)? 138. Are electrical panels unobstructed (3 feet clear)? 139. Are electrical panels labeled? EOSMS 201-1A Laboratory Safety Inspection Checklist Page 9

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