The NORA Summer School October

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1 SCHEDULE FOR THE FIRST NORA SUMMER SCHOOL OCTOBER 2013 Microbial N transformations and NO/N 2 O emissions Date Time Items Teachers Sunday 13/ Arrival, coffee Introduction BIRD S EYE VIEW Nitrogen in food production, the anthropogenic N-cycle and its global impacts N 2 O and NO emissions to the atmosphere what are the consequences? Åsa Frostegård/ /Joachim Lammel Leif Klemedtsson :45-15:30 15:30-15: :30 16:30-17:15 17:15-18:00 BASICS AT ALL SCALES Biochemistry and regulatory biology of denitrification, a brief introduction Biology and ecology of nitrification Nitric oxide scavenging in bacteria various pathways Measuring emissions from soils and ecosystems, why and how? Stable Isotopes and Biogeochemical Cycles in Terrestrial Ecosystems with focus on the N-cycle Molecular microbial ecology- a penny for your thoughts Andrew Gates Jim Prosser Gary Rowley Leif Klemedtsson Tobias Rütting Jim Prosser 18:00- Snacks, posters (mainly by non-nora students) Ca Dinner (served at UMB)

2 Monday 14/ :15 09:15-10:00 10:00-10: : MODEL ORGANISMS AND INDIGENOUS ORGANISMS/COMMUNITIES Soil functional operating range linked to microbial biodiversity and community composition using denitrifiers as model guild Redundancy, resistance and stability of microbial community composition Nitrification at low ph a mystery solved Paracoccus denitrificans, a paradigm organism in denitrification research why continued interest? Sara Hallin Laurent Philippot Graeme Nicol Linda Bergaust Denitrification in a gram positive organism a new model organism? Daniel Mania/Åsa Frostegård :00-15:30 DNA/RNA How can metagenomics help us learn more about nitrogen transformations? Studying the active denitrifiers in soil quantification and sequencing of transcripts Catherine Larose Binbin Liu 15:30- MEASURING GASES - EXERCISES Practical exercises, field fluxes Mass spectrometry; lecture + demonstration ; free evening

3 Tuesday 15/ : MEASURING GASES IN THE FIELD What auxillary data are needed to make a field flux experiment worth doing? Why robotics, and how can we make it a profitable business? Øyvind Overskeid 10:30-11: :00 MEASURING GASES LABSCALE; SOILS AND CULTURES Experimental approaches to study regulation and product stoichiometry of denitrification. Exercises: Group 1) Gas measurements: incubation and gas kinetics; introduction and lab experiment. Nitric oxide scavenging. Åsa Frostegård Binbin Liu/Linda Bergaust 15:00 Group 2) Density gradient cell extraction Swap groups 1 and 2; when convenient Å Frostegård, Rannei Tjåland, Else Aasen 16:00-16:45 17:00-19:30 24:00 Nitric oxide chemistry:autoxidation versus enzymatic scavenging Seminar I discussion of papers Social event

4 Wednesday 16/ :00 14:00-14:45 14:45 15:15- EXCURSION Visit to VEAS wastewater treatment plant; bus leaves at 08:30 from Reenskaug hotel Drøbak SOILS AND CULTURE EXPERIMENTATIONS Real-time PCR basics and calculations + posters Lab exercises and calculations; microscopy, real-time PCR ; free evening Åsa Frostegård/ Janne Utåker Linda Bergaust B Liu, L Bergaust, Å Frostegård, Rannei Tjåland, Else Aasen Thursday 17/ :00 10:00 10:15-11:15 11:15-12:15 Seminar II discussion of papers + posters Oxygen a master variable for microbial N transformations in soils, with an uneven distribution Diffusion at the cellular scale Lars Molstad 12:15-13:00 13:00-14:45 14:45-16:30 17:00-18:00 Exercise Calculations on gas kinetics Wrap-up, concluding seminar. Problems, challenges, possible solutions. Scientific writing. Instructions on report writing. Åsa Frostegård 18:00- Social event - Snacks and drinks; Dinner at UMB Ca 23:00

5 Friday 18/10 Travel home. Optional: Visit UMB Nitrogen group to discuss secondment and plan experiments List of teachers: Local: Åsa Frostegård, Dep. of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Dep. of Plant and Environmental Sciences, Norwegian University of Life Sciences, Dep. of Plant and Environmental Sciences, Norwegian University of Life Sciences Lars Molstad, Dep. of Plant and Environmental Sciences, Norwegian University of Life Sciences Binbin Liu, Dep. of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences Linda Bergaust, Dep. of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences Shahid Nadeem, Dep. of Plant and Environmental Sciences, Norwegian University of Life Sciences Daniel Mania, Dep. of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences Øyvind Overskeid, Adigo AS, Oppegård, Norway Other NORA teachers: Leif Klemedtsson, Department of Earth Sciences, University of Gothenburg, Sweden Tobias Rütting, Department of Earth Sciences, University of Gothenburg, Sweden Sara Hallin, Dep. of Microbiology, Swedish Univ. of Agricultural Sciences, Sweden Laurent Philippot, Soil and Environmental Microbiology, INRA/University of Burgundy, Dijon, France Catherine Larose, Laboratoire Ampère, UMR CNRS 5005, Ecole Centrale de Lyon, France Jim Prosser, Institute of Biological and Environmental Sciences, University of Aberdeen, UK Graeme Nicol, Institute of Biological and Environmental Sciences, University of Aberdeen, UK Andrew Gates, School of Biological Sciences University of East Anglia Norwich, UK Gary Rowley, School of Biological Sciences University of East Anglia Norwich, UK

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