MATERIAL SAFETY DATA SHEET

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1 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: datp Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Water Cas.# Deoxyadenosine 5 - triphosphate disodium salt Molecular weight: Formula: C 10 H 14 N 5 Na 2 O 12 P 3 SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: Irritant HMIS Rating Health: 1 NFPA Rating Health: 1 SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Call a physician. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

2 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. Page 2

3 SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: Irritant. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

4 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: Murine RNase Inhibitor Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Glycerol 50% Cas.# Potassium Chloride < 1% Cas.# K-HEPES < 1% Cas.# NA 4. Dithiothreitol < 1% Cas. # SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: Irritant HMIS Rating Health: 2 NFPA Rating Health: 2 SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Call a physician. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

5 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. Page 2

6 SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: Irritant. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

7 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: dctp Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Water Cas.# Deoxycytidine 5 - triphosphate disodium salt Molecular Formula: C 9 H 14 N 3 Na 2 O 13 P 3 SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: Irritant HMIS Rating Health: 1 NFPA Rating Health: 0 SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Call a physician. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

8 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. Page 2

9 SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: Irritant. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

10 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: dttp Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Water Cas.# Deoxythymidine 5 - triphosphate sodium salt Molecular weight: Formula: C 10 H 16 N 2 O 14 P 3 Na SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: Irritant HMIS Rating Health: 1 NFPA Rating Health: 0 SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Call a physician. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

11 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. Page 2

12 SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: Irritant. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

13 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: dgtp Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Water Cas.# Deoxyguanosine 5 - triphosphate trisodium salt Molecular Formula: C 10 H 13 N 5 Na 3 O 13 P 3 SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: Irritant HMIS Rating Health: 1 NFPA Rating Health: 1 SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Call a physician. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

14 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. Page 2

15 SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: Irritant. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

16 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: M-MuLV Reverse Transcriptase Reaction Buffer Sara 313: No Cas.# None SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Tris-HCl < 1% Cas. # KCl < 1% Cas. # MgCl 2 < 1% Cas. # SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: No known hazards. HMIS Rating Health: 1* NFPA Rating Health: 1 *Additional chronic hazards present. SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

17 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Property Value Temperature or Pressure ph 7.6 Boiling Point Range: Melting Point Range: Freezing Point: Vapor Pressure: Vapor Density: Volatile%: Vicosity: Auto Ignition Temp: Refractive Index: Solubility in Water: Soluble SECTION 10 STABILITY AND REACTIVITY Color: Colorless Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. Page 2

18 SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: None known. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

19 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: M-MuLV Reverse Transcriptase Sara 313: No Cas.# SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT 1. Glycerol 50% Cas.# Sodium Chloride < 1% Cas.# Tris-HCl < 1% Cas.# EDTA < 1% Cas.# NP-40 < 1% Cas.# Dithiothreitol < 1% Cas.# SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OSHA Hazards: No known hazards. HMIS Rating Health: 1* Reactivity: 1 NFPA Rating Health: 1 Reactivity: 1 *Additional chronic hazards present. SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 5 FIRE FIGHTING MEASURES Extinguishing Media: Water spray. Carbon dioxide, dry chemical powder or appropriate foam. Special Fire Fighting Procedures: Wear self contained breathing apparatus and protective clothing to prevent contact with skin and eyes. Fire and Explosion Hazards: Combustible liquid. Emits toxic fumes under fire conditions. Flammability: SECTION 6 ACCIDENTAL RELEASE MEASURES Personal Precautions: Exercise appropriate precaution to minimize direct contact with skin or eyes. Avoid dust formation. Environmental Precautions: Do not let product enter drains. Methods For Cleaning Up: Cover with dry lime, sand, or soda ash. Sweep up and shovel. Place in covered container for disposal. Page 1

20 SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 8 EXPOSURE CONTROLS/PPE Engineering Controls: Safety shower and eye bath. Mechanical exhaust required. Personal Protective Equipment: Gloves suitable to prevent exposure. Hand: Compatible chemical-resistant gloves. Eye: Chemical safety goggles. Skin Specific: Chemical resistant General Hygiene Measures: Wash hands thoroughly after handling. Wash contaminated clothing before use. Respiratory Protection: None required. SECTION 9 PHYSICAL AND CHEMICAL PROPERTIES Physical State: Clear Liquid Color: Colorless Property Value Temperature or Pressure Boiling Point Range: 182 C Melting Point Range: 20 C Flash Point: 320 F, 160 C Method: Closed cup Auto Ignition Temp: 370 C Vapor Pressure: < 1 mmhg 20 C Vapor Density: 3.1 g/l Specific Gravity: Solubility in Water: Soluble SECTION 10 STABILITY AND REACTIVITY Stability: Stable under recommended storage conditions. Materials to Avoid: Acid chlorides, Phosphorus halides, strong oxidizing agents, strong acids, strong reducing agents. Hazardous Decomposition Products: Carbon monoxide, Carbon dioxide, Sulfur dioxides. Hazardous Polymerization: Will not occur. SECTION 11 TOXICOLOGICAL INFORMATION Route of Exposure Skin Absorption: May be harmful if absorbed. Contact: May cause skin irritation. Eye Contact: May cause eye irritation. Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract. Ingestion: May be harmful if swallowed. SECTION 12 ECOLOGICAL INFORMATION Data not available at this time. SECTION 13 DISPOSAL CONSIDERATIONS Appropriate Method of Disposal of Substance or Preparation: Contact a licensed professional waste disposal service to dispose of this material. Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Observe all federal, state and local environmental regulations. Page 2

21 SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None SECTION 15 REGULATORY INFORMATION OSHA Hazards: None known. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 3

22 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: Nuclease-free Water Sara 313: No Cas.# None SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT H 2 O SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OHSA Hazards: No known hazards. HMIS Rating Health: 0* NFPA Rating Health: 0 *Additional chronic hazards present. SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None Page 1

23 SECTION 15 REGULATORY INFORMATION OSHA Hazards: None known. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 2

24 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: Oligo d(t) 23 VN Sara 313: No Cas.# None SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT Sequence: 5' (deoxythymidine) 23 VN'3 (V = A C G) (N=A,C,G,) SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OHSA Hazards: No known hazards. HMIS Rating Health: 1* Reactivity: 1 NFPA Rating Health: 1 Reactivity: 1 *Additional chronic hazards present. SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None Page 1

25 SECTION 15 REGULATORY INFORMATION OSHA Hazards: None known. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 2

26 New England Biolabs 240 County Road Ipswich, MA MATERIAL SAFETY DATA SHEET Telephone: (978) Toll free: (800) Fax: (978) Revision Date: 10/09 ProtoScript M-MuLV Strand cdna Synthesis Kit E6300 SECTION 1 CHEMICAL INFORMATION Product Name: Random Primer Mix Sara 313: No Cas.# None SECTION 2 COMPOSITION/INFORMATION ON INGREDIENT Sequence: 5'd (NNNNNNNNN) '3 (N=A, G, C,T) SECTION 3 HAZARDOUS IDENTIFICATION Emergency Overview: The hazards identified with this product are those associated with the following component: None OHSA Hazards: No known hazards. HMIS Rating Health: 1* Reactivity: 1 NFPA Rating Health: 1 Reactivity: 1 *Additional chronic hazards present. SECTION 4 FIRST AID MEASURES Oral Exposure: If swallowed, wash out mouth with water provided person is conscious. Call a physician. Inhalation Exposure: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician. Dermal Exposure: In case of contact, immediately wash skin with soap and copious amounts of water. Eye Exposure: In case of contact, immediately flush eyes with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician. SECTION 7 HANDLING AND STORAGE Handling: Provide appropriate exhaust ventilation. Storage: Keep tightly closed in a dry and well ventilated place. Store at -20 C. SECTION 14 TRANSPORT INFORMATION DOT Proper Shipping Name: None IMDG Non-hazardous IATA Hazard Label: None Page 1

27 SECTION 15 REGULATORY INFORMATION OSHA Hazards: None known. US Classification and Label Test US Statements: Caution. Avoid contact and inhalation. Target Organ (s): Kidneys. United States Regulatory Information: Sara Listed: No TSCA Inventory Item: Yes Canada Regulatory Information WHMIS Classification: This product has been classified in accordance with the hazard criteria of the CPR and the MSDS contains all the information required by the CPR. DSL: Yes NDSL: No EU Additional Classification S: 23 24/25 Safety Statements: Do not breath vapor. Avoid contact with skin and eyes. Massachusetts Right To Know Components Not subject to the Massachusetts Right to Know Act SECTION 16 OTHER INFORMATION DISCLAIMER For R & D use only. Not for drug, household or other uses. The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. New England Biolabs shall not be held liable for any damage resulting from handling or from contact with the above product. Page 2

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