1 PAGE: 1 of 33 CONTENTS 1.0 SCOPE AND APPLICATION 2.0 METHOD SUMMARY 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING, AND STORAGE 4.0 INTERFERENCES AND POTENTIAL PROBLEMS 5.0 EQUIPMENT/APPARATUS 6.0 REAGENTS 7.0 PROCEDURE 8.0 CALCULATIONS 9.0 QUALITY ASSURANCE/QUALITY CONTROL 10.0 DATA VALIDATION 11.0 HEALTH AND SAFETY 12.0 REFERENCES 13.0 APPENDICES U.S. EPA Contract EP-W
2 PAGE: 2 of SCOPE AND APPLICATION The purpose of this standard operating procedure (SOP) is to describe scientifically sound, legitimate and acceptable procedures to be utilized, as guidelines, for analytical data reduction and manipulation activities pertaining to chromatographic peak integration. This SOP is applicable to all chromatographic data generated at the United States Environmental Protection Agency/ Environmental Response Team (USEPA/ERT) Scientific, Engineering Response and Analytical Services (SERAS) Contract laboratory. 2.0 METHOD SUMMARY In general, procedures in the laboratory use peak area and not peak height for quantitation. Most of the discussion and all the examples provided in this SOP are for determining appropriate peak area. The principles and the specific procedures for completing, documenting and reviewing manual integration generally apply to both peak area and peak height. Chromatographic peaks generated will generally be integrated using automated methods using software provided by the instrument manufacturer. The analyst will review the automated integration and make adjustments and manually integrate the peaks, if necessary. The analyst is expected to use proper judgment in selecting methodology for peak integration based on this SOP. All data manipulations will be documented and peer reviewed to ensure compliance with this SOP. Under no circumstances will data manipulation, such as peak shaving etc. are used for meeting QC criteria. 3.0 SAMPLE PRESERVATION, CONTAINERS, HANDLING, AND STORAGE This section does not apply to this SOP. 4.0 INTERFERENCES AND POTENTIAL PROBLEMS Incorrect results may be obtained due various errors that could result when using the automated peak integration program such as peak splitting, adding area due to a coeluting interferant, failure to detect a peak, excessive peak tailing due to failure of the instrument response to return to baseline or a rise in the baseline, and failure to separate peaks. Failure to follow the procedures outlined in this SOP for manual integration may result in incorrect results. 5.0 EQUIPMENT/APPARATUS This section does not apply to this SOP. 6.0 REAGENTS This section does not apply to this SOP. 7.0 PROCEDURE The USEPA/ERT SERAS laboratory utilizes software developed by instrument manufacturers or third party suppliers to reduce data from an analog signal to a concentration present in the sample. Most of the software used for chromatography is capable of quantitating using either peak area or peak height and employs mathematical algorithms related to the slope of the response to detect the beginning and end of peaks. Depending upon the
3 PAGE: 3 of 33 sophistication of the software package the analyst has flexibility within the parameters of the software to designate internal standards for either quantitation, qualification or both; establish peak thresholds (at an appropriate level above instrument noise); and similar data processing guidelines. The analytical SOPs include the optimal instrument parameters that allow for automatic integration by the data system in most cases. However, regardless of the sophistication of the software, instances occur when the automated software does not integrate a peak correctly. The failure of the software to appropriately integrate a peak is usually obvious from visual inspection of the chromatogram (at an appropriate scale). Various errors can occur which include, but are not limited to, peak splitting, adding area due to a coeluting interferant, failure to detect a peak, excessive peak tailing due to failure of the instrument response to return to baseline or a rise in the baseline, and failure to separate peaks. The software packages invariably provide a procedure where by the analyst can review the individual data file and provide peak specific instructions on integration to correct these problems. This procedure is referred to as Amanual and relies solely upon the experience and judgment of the analyst to determine proper integration for each peak. All data shall be integrated consistently in standards, samples and QC samples. Integration parameters - both automated and manual - shall adhere to valid scientific chromatographic principles. Manual integration will be employed to correct an improper integration performed by the data system and must always include documentation clearly stating the reason the manual integration was performed, the name of the analyst, and the initials of the supervisor approving the manual integration. Under no circumstances should manual integration be performed solely for the purpose of meeting quality control criteria. In other words, peak shaving, peak enhancing, or manipulations of the baseline to achieve these ends must never occur as this results in an improper integration rather than correcting a data system error. Procedures for determining an Aout of state for an analytical system are provided in the associated analytical SOP. With respect to integration procedures, the analyst must pay particular attention to integration problems encountered for the analysis of standards or blanks, which, in theory, should be free of contamination and interfering peaks. Unusual baseline characteristics, unidentified peaks, splitting peaks, excessive tailing or other problems may indicate a need for instrument maintenance or other corrective action. For compounds such as benzoic acid, which produce non-gaussian peaks, the analyst may use automatic integration parameters in the method, which are different from the other compounds in order to obtain proper peak areas. All chromatographic data shall be integrated consistently for all standards, samples and QC samples. Integration parameters, both automated and manual, must adhere to valid scientific chromatographic principles. Manual integration shall be performed to accurately measure the area under the peak and shall not be performed solely for the purpose of meeting quality control criteria. Eliminating part of the subject peak area or including peaks not belonging to the subject peak is inappropriate manipulation of the analytical data and will not be tolerated. An example of improper manual integration is peak shaving or peak enhancement to make failed calibrations, surrogates, or internal standards appear to meet QC criteria. Conducting peak shaving to eliminate part of the subject area or including area counts not belonging to the subject peak is inappropriate manipulation of analytical data. 7.1 Proper Integration of Chromatographic Peaks Appropriate integration includes all the subject peak area and no extraneous area due to noisy baseline, coeluting peaks or changes in baseline configuration. Common integration techniques include baseline-to-baseline, valley-tovalley or a combination of these procedures. Complicated chromatograms with coeluting peaks may require peak
4 PAGE: 4 of 33 skimming or other appropriate techniques to calculate the area. When determining integration procedures the analyst should consider the underlying peak shape and provide an integration, which includes all the peak area of the subject peak while excluding any area due to interfering peaks or a noisy baseline. The following examples provided in Appendix A refer to properly integrated peaks.! Figure 1 is a properly integrated single peak. The peak is symmetrically shaped and exhibits no indication of coelution. The baseline is stable and returns to the same level (i.e., the baseline is flat). This is an example of baseline-to-baseline integration. Peaks of this nature are usually appropriately integrated automatically by the software. On occasion, the analyst must integrate a peak of this nature manually due to a retention time shift, which causes the data system to incorrectly determine that the peak is not a target analyte. The analyst must justify peak identification for such out-of-window peaks, usually based on changes in the retention time of standards.! Figure 2 is a proper integration of several peaks which are not completely resolved (i.e., the response does not return to the baseline between peaks). In this instance the lowest point between the two peaks, the valley, is selected as the appropriate end point for the peaks.! Figure 3 provides several examples of peaks with slight interferences either just prior to or immediately after the target peak. These interfering peaks are not resolved and may be included in the automatic integration. Figure 3 demonstrates examples of proper integration of these peaks.! Figure 4 is an example of a peak, which requires the use of sophisticated software to remove the area due to a co eluting peak. Depending on the sophistication of the data system, it may be possible to remove the additional area. It is necessary that the resulting integration area preserve the Gaussian peak shape. 7.2 Improper Integration of Chromatographic Peaks Inappropriate integration is any integration, either automated or manual, which excludes area, which should be associated with the target peak or includes area not reasonably attributable to the target peak such as area due to a second peak or excessive peak tailing due to a noisy baseline. Intentionally excluding peak area is commonly referred to as peak shaving. The following examples provided in Appendix B illustrate improper integration. Occurrences such as these are unacceptable and prohibited.! Figure 5 provides an example of an improperly integrated peak. The side of the peak has been removed eliminating significant area, which should be included in the peak. This is not an example of removing an excessive area due to peak tailing because the Gaussian shape of the peak has clearly not been preserved in the integration.! Figure 6 is an example of an improperly elevated baseline. This clearly excludes a large area of the peak, which a baseline-to-baseline integration would correct.! Figure 7 is an improperly integrated peak, which includes both elevating the baseline and eliminating the leading and tailing edge of the peak.! Figure 8 is an example of a peak tail, which had not been included in the integration due to a noisy baseline. The tail of the peak should be integrated assuming a Gaussian peak shape as
5 PAGE: 5 of 33 clearly indicated by the shape of the secondary ion trace.! Figure 9 is an example of adding baseline area to a peak. 7.3 Manual correction of erroneous automated data system integrations 8.0 CALCULATIONS Appendix C provides examples of data system integrations that are inappropriate and the manual integration performed to correct the errors.! Figure 10 is an example of a noisy baseline resulting in poor integration by the data system that is attempting to integrate using a valley-to-valley integration procedure. The appropriate integration of the peak eliminates area associated with baseline changes and integrates only the target peak.! Figure 11 is an example of coelution of the tailing edge of a peak resulting in additional area being included in the automated integration. The manual integration is performed to preserve the peak shape and eliminate the additional area.! Figure 12 is an example of automated integration that can occur when detector response is noisy. The baseline is integrated at an excessively high level and the peak is split due to the noise observed at the top. (The peak is shown superimposed over a normal peak for comparison purposes.) The manual integration of this peak includes all the area reasonably attributable to the peak while excluding the noisy baseline.! Figure 13 is an example of automated integration where the baseline has shifted and the data system used the original system baseline to set the integration baseline. Correct integration uses the new baseline. As described in Section QUALITY ASSURANCE/QUALITY CONTROL The analyst must submit a legible copy of all manual integrations that show the entire peak and baseline. The analyst must initial and date the printout of the manual integration, indicating acceptance not only of the procedures used by the analyst to reintegrate the peak but also the reasoning used to determine that reintegration was necessary and adequate. In addition, the following documentation must be submitted:! Data packages must contain the quantitation report and chromatogram (or total-ion chromatogram for GC/MS data) of each standard, blank, sample and QC sample.! GC/MS data: For each target analyte, tentatively identified compound, internal standard and surrogate identified by GC/MS, the analyst must submit mass chromatograms of at least two characteristic ions and a background-subtracted mass spectrum. For standards, matrix spike samples, laboratory control samples and dilutions, detailed reports showing each target compound are not necessary; however, the analyst must submit a summary report (i.e., a quantitation report and a total-ion chromatogram).
6 PAGE: 6 of 33! To ensure appropriate quality control review, all manually integrated analytical data output shall be designated with an If the data system does not automatically flag the values as such, the analyst must clearly mark the report as such DATA VALIDATION Manual integration will be employed only to correct an improper integration performed by the data system and will always include documentation clearly stating the reason the manual integration was performed and the name of the analyst. The entire data package including the manual integration will be peer reviewed in the laboratory, and dated and initialed by the Group Leader, before release to the data validation group. The analytical data package generated will be reviewed and validated in accordance with the applicable SERAS data validation SOP=s, before release HEALTH AND SAFETY This Section does not apply to this SOP REFERENCES USEPA Region 9 SOP #835, AChromatographic Integration Revision 0, July 1, California Military Environmental Coordination Committee, ABest Practices for the Detection and Deterrence of Laboratory March 1997, Version 1.0. United States Environmental Protection Agency, ATest Methods for Evaluating Solid Waste, Method 8000B Determinative Chromatographic SW-846 Third Edition, December APPENDICES
7 PAGE: 7 of 33 Appendices SOP# 1001 January 2000
8 PAGE: 8 of 33 Appendix A Figure 1 Properly integrated single peak SOP#1001 January 2000
9 PAGE: 9 of 33 Figure 1 Properly integrated single peak The peak is symmetrically shaped and exhibits no indication of coelution. The baseline is stable and returns to the same level (i.e., the baseline is flat). This is an example of baseline-to-baseline integration. Peaks of this nature are usually appropriately integrated automatically by the software. On occasion, the analyst must integrate a peak of this nature manually due to a retention time shift that causes the data system to incorrectly determine that the peak is not a target analyte.
10 PAGE: 10 of 33 Appendix A Figure 2 Properly integrated unresolved peaks SOP#1001 January 2000
11 PAGE: 11 of 33 Figure 2 Properly integrated unresolved peaks Proper integration of several peaks which are not completely resolved (i.e., the response does not return to the baseline between peaks). In this instance the lowest point between the two peaks, the valley, is selected as the appropriate end point for the peaks.
12 PAGE: 12 of 33 Appendix A Figure 3 Proper integration to remove interfering peaks SOP#1001 January 2000
13 PAGE: 13 of 33 Figure 3 Proper integration to remove interfering peaks Several examples of peaks with slight interferences either just prior to or immediately after the target peak. These interfering peaks are not resolved and may be included in the automatic integration. Figure A-3 demonstrates examples of proper integration of these peaks.
14 PAGE: 14 of 33 Appendix Figure 4 Peak shape requiring use of peak skimming SOP#1001 January 2000
15 PAGE: 15 of 33 Figure 4 Peak shape requiring use of peak skimming This is an example of a peak that may require the use of more sophisticated software to remove the area due to a co eluting peak. Depending on the sophistication of the data system, it may be possible to remove the additional area. It is necessary that the resulting integration area preserve the Gaussian peak shape.
16 PAGE: 16 of 33 Appendix B Figure 5 Peak shaving by removing tail SOP#1001 January 2000
17 PAGE: 17 of 33 Figure 5 Peak shaving by removing tail. This is an example of an improperly integrated peak. The side of the peak has been removed eliminating significant area that should be included in the peak. This is not an example of removing an excessive area due to peak tailing because the Gaussian shape of the peak has clearly not been preserved in the integration.
18 PAGE: 18 of 33 Appendix B Figure 6 Peak shaving through elevating the baseline SOP#1001 January 2000
19 PAGE: 19 of 33 Figure 6 Peak shaving through elevating the baseline This is an example of an improperly elevated baseline. This clearly excludes a large area of the peak that a baseline-tobaseline integration would correct.
20 PAGE: 20 of 33 Appendix B Figure 7 Gross peak shaving SOP#1001 January 2000
21 PAGE: 21 of 33 Figure 7 Gross peak shaving. This is an improperly integrated peak that includes both elevating the baseline and eliminating the leading and tailing edge of the peak.
22 PAGE: 22 of 33 Appendix B Figure 8 Including area due to a noisy baseline SOP #1001 January 2000
23 PAGE: 23 of 33 Figure 8 Including area due to a noisy baseline This is an example of a peak tail that had not been included in the integration due to a noisy baseline. The tail of the peak should be integrated assuming a Gaussian peak shape as clearly indicated by the shape of the secondary ion trace.
24 PAGE: 24 of 33 Appendix B Figure 9 An example of adding baseline area to a peak SOP #1001 January 2000
25 PAGE: 25 of 33 Figure 9 An example of adding baseline area to a peak
26 PAGE: 26 of 33 Appendix C Figure 10 Noisy baseline. SOP #1001 January 2000
27 PAGE: 27 of 33 Figure 10 Noisy baseline This is an example of a noisy baseline resulting in poor integration by the data system that is attempting to integrate using a valley-to-valley integration procedures. The appropriate integration (shown in the second figure) of the peak eliminates area associated with baseline changes and integrates only the target peak. endix C App Figure 11 Addition of co-eluting peak SOP#1001 January 2000
28 PAGE: 28 of 33 Figure 11 Addition of coeluting peak. This is an example of coelution of the tailing edge of a peak resulting in additional area being included in the automated integration. The manual integration is performed to preserve the peak shape and eliminate the additional area.
29 PAGE: 29 of 33
30 PAGE: 30 of 33 Appendix C Figure 12 Peak splitting. SOP#1001 January 2000
31 PAGE: 31 of 33 Figure 12 Peak splitting. This is an example of automated integration that can occur when detector response is noisy. The baseline is integrated at an excessively high level and the peak is split due to the noise observed at the top. (The peak is shown superimposed over a normal peak for comparison purposes.) The manual integration of this peak includes all the area reasonably attributable to the peak while excluding the noisy baseline.
32 PAGE: 32 of 33 Appendix C Figure 13 Baseline Shift SOP#1001 January 2000
33 PAGE: 33 of 33 Figure 13 Baseline Shift Ion chromatogram where data system used original baseline to integrate peaks after water dip. Correct integration compensates for baseline shift.
The NELAC Institute Manual Integration Assessment Forum January, 2008 Bob Di Rienzo DataChem Laboratories, Inc. Melinda Jacobson City of Phoenix Discussion Topics What Is It Why Do We Need It with Examples
Step-by-Step Analytical Methods Validation and Protocol in the Quality System Compliance Industry BY GHULAM A. SHABIR Introduction Methods Validation: Establishing documented evidence that provides a high
Enhancing GCMS analysis of trace compounds using a new dynamic baseline compensation algorithm to reduce background interference Abstract The advantages of mass spectrometry (MS) in combination with gas
Quantitative & Qualitative HPLC i Wherever you see this symbol, it is important to access the on-line course as there is interactive material that cannot be fully shown in this reference manual. Contents
Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine
Evaluating Laboratory Data Tom Frick Environmental Assessment Section Bureau of Laboratories Overview of Presentation Lab operations review QA requirements MDLs/ PQLs Why do we sample? Protect physical,
New Jersey Department of Environmental Protection Division of Remediation Management Response Site Remediation Program NJDEP- SRP Low Level USEPA TO-15 Method (NJDEP-LLTO-15-3/2007) March 2007 March 2009
Guidance for Industry Q2B Validation of Analytical Procedures: Methodology November 1996 ICH Guidance for Industry Q2B Validation of Analytical Procedures: Methodology Additional copies are available from:
Medical Cannabis Laboratory Approval Program Application Process and Required Documentation After the publication of the Medical Cannabis Laboratory Application, currently expected by February 16, 2015,
INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE ICH HARMONISED TRIPARTITE GUIDELINE VALIDATION OF ANALYTICAL PROCEDURES: TEXT AND METHODOLOGY
European Medicines Agency June 1995 CPMP/ICH/381/95 ICH Topic Q 2 (R1) Validation of Analytical Procedures: Text and Methodology Step 5 NOTE FOR GUIDANCE ON VALIDATION OF ANALYTICAL PROCEDURES: TEXT AND
Alignment and Preprocessing for Data Analysis Preprocessing tools for chromatography Basics of alignment GC FID (D) data and issues PCA F Ratios GC MS (D) data and issues PCA F Ratios PARAFAC Piecewise
APPENDIX C. DATA VALIDATION REPORT T-117 Upland Investigation Upland Investigation Data Report Appendices PROJECT NARRATIVE Basis for the Data Validation This report summarizes the results of the validation
Introduction This document is designed to help our clients understand the quality control requirements and limitations of data reporting. There are three sections to this document. The first section will
Signal, Noise, and Detection Limits in Mass Spectrometry Technical Note Chemical Analysis Group Authors Greg Wells, Harry Prest, and Charles William Russ IV, Agilent Technologies, Inc. 2850 Centerville
MultiQuant Software Version 3.0 for Accurate Quantification of Clinical Research and Forensic Samples Fast and Efficient Data Review, with Automatic Flagging of Outlier Results Adrian M. Taylor and Michael
Strategies for Developing Optimal Synchronous SIM-Scan Acquisition Methods AutoSIM/Scan Setup and Rapid SIM Technical Overview Introduction The 5975A and B series mass selective detectors (MSDs) provide
Analytical Data Package Reports TriMatrix offers a variety of analytical data packages formatted to meet the reporting needs and varying data quality objectives of its clients. The data packages offered
Ecology Quality Assurance Glossary Edited by William Kammin, Ecology Quality Assurance Officer Accreditation - A certification process for laboratories, designed to evaluate and document a lab s ability
Page 1 of 12 Sections Included in this Document and Change History 1. Purpose 2. Scope 3. Responsibilities 4. Background 5. References 6. Procedure/(6. B changed Division of Field Science and DFS to Office
Empower Software Data Acquisition and Processing Theory Guide 34 Maple Street Milford, MA 01757 71500031209, Revision B NOTICE The information in this document is subject to change without notice and should
Determination of Formic Acid, Acetic Acid and Propionic Acid Contents in FAME - Blended Diesel Fuels (Draft) Water Extraction Ion Chromatography Method (WARNING The use of this standard may involve hazardous
Minnesota Pollution Control Agency (MPCA) Laboratory Certification Program Manual Revision 0 This manual was written with plain language in mind. When we or us is used, this is referring to the Certification
EnviroLab Forms Production Quick Reference Guide This quick reference guide describes the Production Mode tasks assigned to the roles of Manager, Supervisor, Technician, and QAQC. Contents Production Mode
APPENDIX N Data Validation Using Data Descriptors Data validation is often defined by six data descriptors: 1) reports to decision maker 2) documentation 3) data sources 4) analytical method and detection
Radioanalytical Data Validation Issues and Solutions Presented by: Terry McKibbin and Kevin Harmon Validata Chemical Services, Inc. Purpose of Data Validation The Presentation is Based on this Goal for
Thermo Scientific Dionex Chromeleon 7 Chromatography Data System Software Frank Tontala, Thermo Fisher Scientific, Germering, Germany Technical Note 708 Key Words Chromeleon Chromatography Data System,
APPENDIX 7-B SUGGESTED OUTLINE OF A QUALITY ASSURANCE PROJECT PLAN This outline is recommended for use by UST consultants/contractors in preparing a generic Quality Assurance Project Plan (QAPP) for use
LC-MS/MS, the new reference method for mycotoxin analysis What is necessary for the implementation of the multi-residue mycotoxin method in an analytical laboratory? Azel Swemmer firstname.lastname@example.org AFMA
Chemistry 321, Experiment 8: Quantitation of caffeine from a beverage using gas chromatography INTRODUCTION The analysis of soft drinks for caffeine was able to be performed using UV-Vis. The complex sample
Page:1 of 8 Alkalinity, Carbonate and BiCarbonate Analysis by Titrimetric Procedure 1.0 Purpose This procedure provides for analysis for water samples containing Alkalinity. Alkalinity is a total of Carbonate,
Date Issued: July 28, 2014 Pace Analytical e-report Report prepared for: ATLANTIC TESTING LABORATORIES, LTD 22 CORPORATE DR CLIFTON PARK, NY 12065 CONTACT: Derek Converse ----------------------------------------------
Millennium/Empower Advance Training for Teva Dr. Shulamit Levin email@example.com Reviewing Data 1 Review Multiple Channels The list of channels is in the 2D channels view 2 The results of the integration
Highly Selective Analysis of Steroid Biomarkers using SelexION Ion Mobility Technology Hua-Fen Liu, Witold Woroniecki, Doina Caraiman, and Yves LeBlanc AB SCIEX, Foster City, USA One of the most challenging
Quantitative work in HPLC Dr. Shulamit Levin Medtechnica www.forumsci.co.il/hplc Dr. Shulamit Levin, Medtechnica 1 Quantitative work in HPLC Dr. Shulamit Levin Medtechnica Data Handling Analytical Chemistry
application Note Gas Chromatography/ Mass Spectrometry Author A. Tipler, Senior Scientist PerkinElmer, Inc. Shelton, CT 06484 USA The Determination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes
SoBran, Inc. Corporate Quality Management Plan for Projects and Activities This proposal or quotation includes data that shall not be disclosed outside the government and shall not be duplicated, used,
TROUBLESHOOTING GUIDE HPLC Before starting any troubleshooting, whether it is related to instruments or columns, it is essential that safe laboratory practices be observed. The chemical and physical properties
METHOD 9075 TEST METHOD FOR TOTAL CHLORINE IN NEW AND USED PETROLEUM PRODUCTS BY X-RAY FLUORESCENCE SPECTROMETRY (XRF) 1.0 SCOPE AND APPLICATION 1.1 This test method covers the determination of total chlorine
The First Quantitative Analysis of Alkylated PAH and PASH by GCxGC/MS and its Implications on Weathering Studies INEF Penn State Conference 2013 Albert Robbat, Jr. and Patrick Antle Tufts University, Chemistry
INTRODUCTION ITS REWORK DATA VERIFICATION REPORT for samples collected from CAMP STANLEY STORAGE ACTIVITY BOERNE, TEXAS Data Verifiers: Michelle Wolfe & Tammy Chang Parsons The following data validation
State Water Resources Control Board Environmental Laboratory Accreditation Program P.O. Box 100, Floor 24 850 Marina Bay Parkway, Build. P 500 N Central Ave, Suite 500 Sacramento, CA 95812-0100 Richmond,
METHOD 8082A POLYCHLORINATED BIPHENYLS (PCBs) BY GAS CHROMATOGRAPHY SW-846 is not intended to be an analytical training manual. Therefore, method procedures are written based on the assumption that they
Investigating the use of the AB SCIEX TripleTOF 4600 LC/MS/MS System for High Throughput Screening of Synthetic Cannabinoids/Metabolites in Human Urine AB SCIEX MPX -2 High Throughput TripleTOF 4600 LC/MS/MS
CONFIRMATION OF ZOLPIDEM BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY 9.1 POLICY This test method may be used to confirm the presence of zolpidem (ZOL), with diazepam-d 5 (DZP-d 5 ) internal standard, in
Analytical Method Validation A. Es-haghi Ph.D. Dept. of Physico chemistry vaccine and serum research institute firstname.lastname@example.org http://www.rvsri.ir/ Introduction Test procedures for assessment of the
Final 7/96 APPENDIX E - PERFORMANCE EVALUATION AUDIT APPENDIX E PERFORMANCE EVALUATION AUDIT CHECKLIST EXAMPLE APPENDIX E - PERFORMANCE EVALUATION AUDIT Final 7/96 This page is intentionally left blank.
Tutorial 4.6 Gamma Spectrum Analysis Slide 1. Gamma Spectrum Analysis In this module, we will apply the concepts that were discussed in Tutorial 4.1, Interactions of Radiation with Matter. Slide 2. Learning
C146-E175A Technical Report Automatic Identification and Semi-quantitative Analysis of Psychotropic Drugs in Serum Using GC/MS Forensic Toxicological Database Hitoshi Tsuchihashi 1 Abstract: A sample consisting
DIVISION OF ENVIRONMENT QUALITY MANAGEMENT PLAN PART III: DRY CLEANING PROGRAM QUALITY ASSURANCE MANAGEMENT PLAN Revision 3 January 8, 2013 Kansas Department of Health and Environment Division of Environment
Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed
The Wastewater Treatment Plant Operators Guide to Biosolids Sampling Plans CHAPTER 5 DATA QUALITY OBJECTIVES Goals of the Sampling Plan ESSENTIAL ELEMENTS OF A SAMPLING PLAN Description of the Facility
Drinking Water Analytical Method and Program Requirements: Roles and Responsibilities, Analytical Method Approval, and Effective Oversight National Environmental Monitoring Conference July 13, 2015 Daniel
FORENSIC TOXICOLOGY LABORATORY OFFICE OF CHIEF MEDICAL EXAMINER CITY OF NEW YORK VOLATILES ANALYSIS by GCMS HEADSPACE ANALYSIS PRINCIPLE This method confirms the identity of volatile compounds in biological
ACCUTEST LABORATORIES Data Integrity & Technical Ethics January 2012 What is Data Integrity? Data that has been produced to the ethical standards of the industry, which is traceable and defensible. What
NC SBI QUALITY ASSURANCE PROGRAM for the SBI Reviewed by: Deputy Assistant Director Bill Weis Date: Approved by: Assistant Director Jerry Richardson Date: Originating Unit: SBI Effective Date: July 25,
Attention: Angela Mazza / Kathleen Wilson Catholic Education Centre 80 Sheppard Ave E North York, ON M2N 6E8 Your Project #: ANNUNCIATION Your C.O.C. #: na Report #: R3046860 Version: 1 MAXXAM JOB #: B488250
CHAPTER 1 INTRODUCTION 1. Classification of Analytical Methods 2. Types of Instrumental Methods 3. Instruments for Analysis Information Flow and Processing/ Transformation - Modules of an Instrument 4.
GLOSSARY Assessment - the evaluation process used to measure the performance or effectiveness of a system and its elements. As used here, assessment is an all-inclusive term used to denote any of the following:
CHEM 311L Quantitative Analysis Laboratory Version 1.1 An HPLC Analysis of Sweeteners in Beverages In this laboratory exercise we will perform a separation of the components of diet soft drinks using reversed-phase
Designing a CDS Landscape that Ensures You Address the Latest Challenges of the Regulatory Bodies with Ease and Confidence Heather Longden Senior Marketing Manager, Informatics Regulatory Compliance 2015
Data Analysis Software for VISION, BioCAD 700E, SPRINT, and INTEGRAL Workstations Version 3 Series Software Getting Started Guide DRAFT August 10, 2001 2:47 pm DASgsg_Title.fm Copyright 1998, 2001, Applied
Analysis of the Vitamin B Complex in Infant Formula Samples by LC-MS/MS Stephen Lock 1 and Matthew Noestheden 2 1 AB SCIEX Warrington, Cheshire (UK), 2 AB SCIEX Concord, Ontario (Canada) Overview A rapid,
Optimizing the Post-implementation Monitoring of a LC-MS/MS Method Julianne Cook Botelho, PhD Centers for Disease Control and Prevention Atlanta, GA AACC- Mass Spectrometry in the Clinical Lab: Best Practice
1 Gas Chromatography/Mass Spectroscopy (GC/MS/MS) Background Information Instructions for the Operation of the Varian CP-3800 Gas Chromatograph/ Varian Saturn 2200 GC/MS/MS See the Cary Eclipse Software
Enhanced Diode Array Detector Sensitivity and Automated Peak Purity Control Technical Note Introduction The most widely used detection technique for HPLC analysis is UV absorption. Over the decades, single
CHAPTER 252. ENVIRONMENTAL LABORATORY ACCREDITATION Subchapter A. GENERAL PROVISIONS B. APPLICATION, FEES AND SUPPORTING DOCUMENTS C. GENERAL STANDARDS FOR ACCREDITATION D. QUALITY ASSURANCE AND QUALITY
Liquid chromatography tandem mass spectrometry (LC-MSMS) - the primary tool for trace contaminant analysis Ivo Leito University of Tartu Institute of Chemistry Estonia email@example.com HO H HO H 1st WYSC
Application Notes # LCMS-35 esquire series Application of LC/APCI Ion Trap Tandem Mass Spectrometry for the Multiresidue Analysis of Pesticides in Water An LC-APCI-MS/MS method using an ion trap system
Accepted 2005 Process liquors from bleach plants Dissolved and precipitated oxalate Using Ion Chromatography 0 Introduction In bleach plants of pulp mills with a high degree of system closure, there is
A Navigation through the Tracefinder Software Structure and Workflow Options Frans Schoutsen Pesticide Symposium Prague 27 April 2015 Kings day in The Netherlands 1 Index Introduction Acquisition, Method
UPLC-MS/MS Analysis of in Plasma for Clinical Research Dominic Foley and Lisa Calton Waters Corporation, Wilmslow, UK APPLICATION BENEFITS Analytical selectivity improves reproducibility through removal
Analysis of Free Bromate Ions in Tap Water using an ACQUITY UPLC BEH Amide Column Sachiki Shimizu, FUJIFILM Fine Chemicals Co., Ltd., Kanagawa, Japan Kenneth J. Fountain, Kevin Jenkins, and Yoko Tsuda,
AppNote 9/2006 Target GC-MS Analysis using Accelerated Column Heating and Interactive Deconvolution Software Albert Robbat Tufts University, Chemistry Department, Medford, MA 02155, USA Andreas Hoffmann
Auditing Chromatographic Electronic Data Jennifer Bravo, M.S. QA Manager Agilux Laboratories Outline Raw data paper or electronic record? Controls for electronic data Auditing electronic records Warning