Oxi/FermPluri Test CODEBOOK. System for the identification of Gram negative, oxidase positive bacteria. Ref
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1 Oxi/FermPluri Test ystem for the identification of Gram negative, oxidase positive bacteria. CODEBOOK ef iofilchem.r.l. Microbiology Products
2 F52067 ev.0/
3 Oxi/FermPluri Test CODEBOOK Index Pag. Biocode Database 1 Appendix 1: ist of abbreviations used in the Biocode Database. 17 Appendix 2: ecommended procedures for confirmatory tests. 18 Appendix 3: Availability of media and reagents needed for the performance of the confirmatory tests. 20 Important Notes Before using the Oxi/FermPluriTest, please read the instructions for use. iofilchem.r.l. Microbiology Products
4 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Acinetobacter lwoffii Kingella denitrificans Empedobacter brevis Oligella urethralis phingomonas paucimobilis Pasteurella multocida Moraxella spp. Pseudomonas spp. Alcaligenes faecalis Acinetobacter lwoffii Pseudomonas alcaligenes Pseudomonas spp. Pseudomonas alcaligenes Alcaligenes faecalis Acinetobacter lwoffii Moraxella spp. Pseudomonas alcaligenes hizobium (Agrobacterium) radiobacter Bordetella bronchiseptica Oligella urethralis Acinetobacter lwoffii tenotrophomonas maltophilia Bordetella bronchiseptica Myroides odoratus Moraxella spp. Alcaligenes spp. Delftia acidovorans Ochrobactrum anthropi Brevundimonas diminuta Brevundimonas vesicularis tenotrophomonas maltophilia Brevundimonas diminuta Flavobacterium spp. hewanella putrefaciens hewanella putrefaciens 1 45% 42% * * 44% 34% 19% 82% 16% 27% 24% 18% 83% 13% 56% 34% 10% Cat /() pol/ pol peri peri peri pol peri Plump cocci DNA DNA v DNA TOB /()
5 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET tenotrophomonas maltophilia hewanella putrefaciens tenotrophomonas maltophilia tenotrophomonas maltophilia Pseudomonas stutzeri Pseudomonas fluorescens phingobacterium multivorum Achromobacter spp. Myroides odoratus Flavobacterium spp. Brevundimonas diminuta Flavobacterium spp Flavobacterium spp Chryseobacterium meningosepticum Flavobacterium spp phingobacterium multivorum Chryseobacterium meningosepticum hizobium (Agrobacterium) radiobacter Acinetobacter baumannii/calcoaceticus Pseudomonas stutzeri Achromobacter spp. alstonia pickettii Achromobacter spp. Brevundimonas diminuta Pseudomonas stutzeri Brevundimonas diminuta Acinetobacter baumannii/calcoaceticus Pseudomonas oryzihabitans 2 39% 25% 9% 45% 26% 13% 42% 38% 19% 86% 14% 64% 31% 56% 23% 20% 89% 16% 84% 16% Cet Cet ur Ur NO3 McC McC Cet DNA DNA PB
6 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas fluorescens hizobium (Agrobacterium) radiobacter Acinetobacter baumannii/calcoaceticus Pseudomonas oryzihabitans alstonia pickettii Achromobacter spp Pseudomonas oryzihabitans Achromobacter spp. Pseudomonas oryzihabitans Achromobacter spp. alstonia pickettii hizobium (Agrobacterium) radiobacter Achromobacter spp Flavobacterium spp Flavobacterium spp Flavobacterium spp Flavobacterium spp Flavobacterium spp Flavobacterium spp Chryseobacterium meningosepticum Flavobacterium spp Flavobacterium spp Flavobacterium spp Chryseobacterium meningosepticum Chryseobacterium meningosepticum Chryseobacteriumindologenes 47% 25% 12% 63% 23% 52% 41% 7% 54% 46% Ur Ur DNA 1 Cet TOB 3
7 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Brevundimonas vesicularis Pasteurella multocida Moraxella spp Brevundimonas vesicularis Moraxella spp. phingobacterium multivorum Achromobacter spp. Comamonas testosteroni/terrigena Brevundimonas diminuta Brevundimonas vesicularis Brevundimonas vesicularis Achromobacter spp Moraxella spp. Alcaligenes faecalis Pseudomonas stutzeri Pseudomonas stutzeri Alcaligenes spp hewanella putrefaciens Moraxella spp. Alcaligenes faecalis Myroides odoratus Alcaligenes spp. Pseudomonas spp Brevundimonas diminuta Brevundimonas diminuta hewanella putrefaciens hewanella putrefaciens hewanella putrefaciens Pseudomonas stutzeri Achromobacter xylosoxidans subsp. denitrificans alstonia pickettii Myroides odoratus Pseudomonas stutzeri 4 67% 23% 79% 11% 10% 60% 40% 51% 16% 15% 52% 35% 46% 41% 10% 51% 36% 12% 88% 12% 88% 9% 83% 14% DNA Cet ßhem DNA
8 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET alstonia pickettii Myroides odoratus Pseudomonas stutzeri Pseudomonas stutzeri Pseudomonas stutzeri alstonia pickettii alstonia pickettii Brevundimonas diminuta Flavobacterium spp. Pasteurella multocida tenotrophomonas maltophilia Flavobacterium spp phingobacterium multivorum hizobium (Agrobacterium) radiobacter tenotrophomonas maltophilia Achromobacter spp Bordetella bronchiseptica phingobacterium multivorum tenotrophomonas maltophilia tenotrophomonas maltophilia Acinetobacter baumannii/calcoaceticus Achromobacter spp. Acinetobacter baumannii/calcoaceticus phingobacterium multivorum Chryseobacterium meningosepticum Achromobacter spp phingobacterium multivorum Acinetobacter baumannii/calcoaceticus Achromobacter spp Acinetobacter baumannii/calcoaceticus 5 85% 14% 74% 24% 46% 40% 14% 54% 44% 83% 16% 69% 26% 53% 47% 79% 21% Cet Cet PB
9 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas fluorescens Achromobacter spp Acinetobacter baumannii/calcoaceticus Achromobacter spp hizobium (Agrobacterium) radiobacter Pasteurella multocida Flavobacterium spp Flavobacterium spp Pasteurella multocida Vibrio alginolyticus Achromobacter spp phingobacterium multivorum phingobacterium multivorum Achromobacter spp Aeromonas hydrophila Aeromonas hydrophila Pasteurella multocida Pseudomonas stutzeri Alcaligenes spp Acinetobacter lwoffii Pseudomonas spp. Alcaligenes faecalis tenotrophomonas maltophilia Acinetobacter lwoffii Delftia acidovorans Pseudomonas alcaligenes Achromobacter xylosoxidans subsp. denitrificans tenotrophomonas maltophilia Achromobacter xylosoxidans subsp. denitrificans Delftia acidovorans Bordetella bronchiseptica tenotrophomonas maltophilia 79% 21% 48% 47% 88% 12% 53% 44% 61% 23% 85% 15% 32% 20% 13% 34% 26% 17% Ur TCB TOB Ur 6
10 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas spp. * tenotrophomonas maltophilia hewanella putrefaciens Burkholderia cepacia Brevundimonas diminuta tenotrophomonas maltophilia Burkholderia cepacia tenotrophomonas maltophilia tenotrophomonas maltophilia tenotrophomonas maltophilia Burkholderia cepacia tenotrophomonas maltophilia tenotrophomonas maltophilia tenotrophomonas maltophilia Burkholderia cepacia Acinetobacter baumannii/calcoaceticus Burkholderia cepacia Burkholderia cepacia Pseudomonas mendocina Burkholderia cepacia Burkholderia cepacia Burkholderia cepacia Acinetobacter baumannii/calcoaceticus Burkholderia cepacia Acinetobacter baumannii/calcoaceticus alstonia pickettii % 40% 65% 32% 84% 15% 37% 32% 22% 30% 28% 25% 32% 28% 22% Cet CA CA PB
11 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Burkholderia cepacia Burkholderia cepacia Vibrio parahaemolyticus Brevundimonas diminuta 69% 31% Vibrio cholerae serol Alcaligenes faecalis Comamonas testosteroni/terrigena Delftia acidovorans Alcaligenes faecalis (odorans) Alcaligenes faecalis Achromobacter xylosoxidans subsp. denitrificans Alcaligenes spp Brevundimonas diminuta hewanella putrefaciens hewanella putrefaciens Pseudomonas stutzeri Pseudomonas mendocina Pseudomonas mendocina alstonia pickettii Pseudomonas stutzeri Pseudomonas mendocina alstonia pickettii Pseudomonas mendocina alstonia pickettii tenotrophomonas maltophilia tenotrophomonas maltophilia tenotrophomonas maltophilia 63% 14% 10% 67% 17% 15% 81% 17% 80% 20% 55% 37% 70% 20% 10% v Cet DNA Cet Cet TOB TE 8
12 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Bordetella bronchiseptica Delftia acidovorans tenotrophomonas maltophilia tenotrophomonas maltophilia tenotrophomonas maltophilia tenotrophomonas maltophilia Acinetobacter baumannii/calcoaceticus Acinetobacter baumannii/calcoaceticus Plesiomonas shigelloides Vibrio parahaemolyticus Vibrio alginolyticus Vibrio alginolyticus Pseudomonas fluorescens Pseudomonas fluorescens Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa 9 87% 13% 68% 17% 11% 70% 12% 10% 82% 18% 53% 36% 11% 62% 33% 42C 42C TOB TOB TOB
13 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas fluorescens Pseudomonas fluorescens Achromobacter xylosoxidans subsp. denitrificans Bordetella bronchiseptica Pseudomonas mendocina Burkholderia cepacia Pseudomonas mendocina Burkholderia cepacia Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Achromobacter xylosoxidans subsp. denitrificans Alcaligenes spp Pseudomonas mendocina Pseudomonas mendocina Pseudomonas mendocina Pseudomonas aeruginosa 47% 24% 20% 57% 29% 65% 21% 8% 52% 40% 89% 9% 67% 28% 42C 42C 42C 42C Burkholderia pseudomallei serol Burkholderia pseudomallei serol CA TOB 10
14 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Burkholderia pseudomallei serol Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas aeruginosa Pseudomonas mendocina Pseudomonas fluorescens Plesiomonas shigelloides Plesiomonas shigelloides Plesiomonas shigelloides Pseudomonas mendocina Brevundimonas vesicularis phingomonas paucimobilis phingomonas paucimobilis Flavobacterium spp Achromobacter spp Brevundimonas diminuta Brevundimonas vesicularis Flavobacterium spp Flavobacterium spp Pseudomonas fluorescens Chryseobacterium meningosepticum Brevundimonas vesicularis Acinetobacter baumannii/calcoaceticus Acinetobacter haemolyticus 66% 34% 50% 35% 59% 36% 56% 37% 7% 72% 16% 42C ßhem 11
15 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas fluorescens Acinetobacter baumannii/calcoaceticus Achromobacter spp Flavobacterium spp Chryseobacterium indologenes Chryseobacterium meningosepticum Chryseobacterium indologenes Flavobacterium spp Flavobacterium spp Chryseobacterium meningosepticum Vibrio parahaemolyticus Chryseobacterium indologenes Chryseobacterium indologenes Achromobacter spp Chryseobacterium indologenes Achromobacter spp Acinetobacter baumannii/calcoaceticus Achromobacter spp Acinetobacter baumannii/calcoaceticus Pseudomonas fluorescens Acinetobacter baumannii/calcoaceticus Achromobacter spp Achromobacter spp Chryseobacterium meningosepticum Flavobacterium spp Vibrio parahaemolyticus Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Aeromonas caviae Aeromonas hydrophila 12 89% 11% 75% 25% 86% 10% 71% 15% 14% Ur DNA ßhem DNA
16 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Burkholderia cepacia Burkholderia cepacia Burkholderia cepacia Pseudomonas mendocina Burkholderia cepacia Acinetobacter baumannii/calcoaceticus Pseudomonas fluorescens Burkholderia cepacia Acinetobacter baumannii/calcoaceticus Pseudomonas fluorescens Burkholderia cepacia Burkholderia cepacia Burkholderia cepacia Vibrio parahaemolyticus Vibrio parahaemolyticus Vibrio vulnificus Vibrio cholerae Vibrio parahaemolyticus Vibrio parahaemolyticus Vibrio cholerae Vibrio mimicus Vibrio parahaemolyticus 68% 14% 10% 50% 25% 24% 55% 39% 68% 32% 78% 22% CA TOB ali serol VP var PB var serol 13
17 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas mendocina Pseudomonas fluorescens Pseudomonas mendocina Pseudomonas fluorescens Vibrio alginolyticus Vibrio alginolyticus Acinetobacter baumannii/calcoaceticus Plesiomonas shigelloides Vibrio parahaemolyticus Plesiomonas shigelloides Vibrio parahaemolyticus Plesiomonas shigelloides Vibrio parahaemolyticus Vibrio alginolyticus Plesiomonas shigelloides Vibrio parahaemolyticus Vibrio vulnificus Vibrio parahaemolyticus Vibrio parahaemolyticus Vibrio alginolyticus Vibrio alginolyticus Vibrio alginolyticus Vibrio alginolyticus Aeromonas hydrophila Aeromonas hydrophila Vibrio alginolyticus Aeromonas hydrophila Aeromonas hydrophila Vibrio alginolyticus Vibrio alginolyticus 68% 14% 90% 10% 82% 18% 79% 21% 72% 28% 68% 32% 87% 13% 74% 26% 52% 48% 86% 14% 42C 42C Vibrio cholerae serol NO NO NO 14
18 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Vibrio alginolyticus Aeromonas hydrophila Aeromonas hydrophila Vibrio alginolyticus Vibrio cholerae Vibrio mimicus Aeromonas veronii bv. veronii Aeromonas hydrophila Vibrio parahaemolyticus Vibrio parahaemolyticus Pseudomonas fluorescens Pseudomonas fluorescens Pseudomonas fluorescens Chromobacterium violaceum Aeromonas caviae Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Aeromonas veronii bv. veronii bv. sobria Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Aeromonas hydrophila Burkholderia pseudomallei Burkholderia cepacia 56% 44% 71% 29% 52% 48% 66% 34% 57% 43% 88% 8% NO NO serol Col violet ali TE ONPG serol Burkholderia pseudomallei serol 15 VP PB / /
19 iofilchem Oxi/FermPluriTest Codebook ev.0/ BIOCODE OGANIM % CONFIMATOY TET Pseudomonas fluorescens Pseudomonas fluorescens Burkholderia cepacia Pseudomonas mendocina Pseudomonas mendocina Pseudomonas mendocina Plesiomonas shigelloides Aeromonas hydrophila Aeromonas hydrophila Burkholderia cepacia Aeromonas hydrophila Burkholderia cepacia 65% 33% 74% 25% 78% 22% 78% 22% 42C 42C 16
20 Abbreviation iofilchem Oxi/FermPluriTest Codebook ev.0/ Appendix 1. ist of abbreviations used in the Biocode Database. Explanation % Percent in the headline of the biocode database indicates likelihood in % 42C Growth at 42 C CA Cat Cet CF Tests Col DNA McC NO3 NO ONPG PB Ur ali erol Amikacin, diskdiffusion test on Mueller Hinton II agar Carbenicillin, diskdiffusion test on Mueller Hinton II agar Catalase test with H 2 O 2, Catalase/OxyTest Cetrimide, growth Pseudomonas (Cetrimide) Agar Confirmatory test Colony coloration (nondiffusible pigment) on Tryptic oy Agar after 23 days incubation at optimal growth temperature DNase test, DNase Test Agar Type of flagellation: either pol (polar) or peri (peritrichous) atinase, Nutrient atin Profile number determined with limited number of strains only Growth on MacConkey II Agar ility Nitrate reduction to nitrite, Nitrate Test Novobiocin, diskdiffusion test on Mueller Hinton II agar ONPG reaction, 2nitrophenylßDglucopyranoside ymyxin B, 2nitrophenyl beta dglucopyranoside esistant apid Urease, 1824 h incubation usceptible Acid formation from salicin, alicin Test Confirm identification with serological tests ßhem Beta hemolysis on Colubia Agar (heep Blood 5%) TCB TE TOB Var VP Growth on TCB Agar Tetracyclin, diskdiffusion test on Mueller Hinton II agar Tobramycin, diskdiffusion test on Mueller Hinton II agar Variable result (either o, o ) possible VogesProskauer reaction, VP Test Kit Yellow pigment on Tryptic oy Agar 17
21 iofilchem Oxi/FermPluriTest Codebook ev.0/ Appendix 2. ecommended Procedures for Confirmatory Tests. 1. Growth at 42 C (42C) Prepare a light suspension in a suitable liquid medium (e.g. Tryptic oy Broth) with not more than 1 colony of the isolate and incubate at 42 C (preferably in a water bath) for 18 to 24 hours. Growth is indicated by an increase of turbidity of the medium. 2. Antimicrobial disc diffusion tests Conventional disc diffusion procedures and the zone size limits usually used should be applied (use Mueller Hinton II Agar plates). Consider both intermediate and susceptible zones as susceptible () for this purpose. The following discs are used: Abbreviation Antimicrobial Concentration Amikacin 30 µg CA Carbenicillin 100 µg NO Novobiocin 30 µg PB ymyxin B 300 IU TE Tetracycline 30 µg TOB Tobramycin 10 µg 3. Catalase (Cat) Apply a drop of freshly prepared 3% hydrogen peroxide (H2O2) to a glass slide. With a loop, remove a colony of the isolate from a 24 to 48 h plate and mix it with the hydrogen peroxide drop. Bubble formation and foaming indicates a positive reaction. Alternatively, a drop of 3% hydrogen peroxide may be applied to the colonies grown on Tryptic oy Agar. Do not apply the hydrogen peroxide to blood agars, false positive reaction may occur. Alternatively use Catalase/OxyTest according to the procedure reported on the instructions sheet. 4. Cetrimide (Cet) treak the isolate on Pseudomonas (Cetrimide) Agar. Incubate for 18 to 24 hours at the optimal temperature of the isolate. Growth on this medium means positive. 5. Colony coloration (Col) treak the isolate on Trypticase oy Agar and incubate for 2 to 3 days at the optimal temperature. Inspect for colony coloration (normal, nonpigmented colonies appear white, gray, or pale yellow). Pigmented colonies appear yellow to brownish or violet, depending on the species. 6. DNase activity (DNA) treak the isolate on DNase Test Agar. Incubate for 2 days at 36 / 1 C. Flood the plate with 1 N HCl and wait for about 1 min. A clear zone is produced around DNase positive colonies while a homogeneously turbid medium indicates DNase negativity. 7. stain A variety of methods is given in the literature, all of which require some experience. 8. atinase () tabinoculate a tube with Nutrient atin with a heavy inoculum and incubate at room temperature. A positive test is indicated by liquefaction of the medium at the surface within 18 to 48 hours. Negative reaction: no liquefaction. 9. Growth on MacConkey II Agar (McC) Prepare a light suspension of the isolate in saline or Tryptic oy Broth and immediately inoculate a plate of MacConkey II Agar with a loopful of this suspension. Incubate at 36/1 C for 20 to 24 hours. If the strain shows poor growth on a blood plate incubated at 36/1 C, incubate the MacConkey II Agar plate at a lower temperature, but not longer than 48 hours. 18
22 iofilchem Oxi/FermPluriTest Codebook ev.0/ ility () Place a drop of a broth culture of the isolate which has been incubated for 6 to 8 hours at 25 C onto a slide. Place a cover slip onto the slide and observe by phase contrast microscopy (oil immersion) for motile bacteria. A regular oil immersion objective may also be used if the microscopic field can be darkened. True motility is shown by a rapid change in position of individual bacterial cells relative to other cells. 11. Nitrate reduction (NO3) Inoculate a tube of Nitrate Broth with a loopful of organisms taken from a pure culture. Incubate for 18 to 48 h at 32 to 36 C with cap slightly loosened. Evaluate the test by adding 0.5 ml of each of the two nitrate reagents to detect the formation of nitrite from nitrate (red coloration). If the reaction is negative (colorless), add some zinc dust to detect unused nitrate. If the color turns red after the addition of zinc dust, nitrate has not been used (negative reaction); if it remains colorless after the addition of zinc dust, nitrate has been reduced passed nitrite (denitrification, positive reaction). Alternatively use Nitrate Test according to the procedure reported on the instructions sheet. 12. ONPG The hydrolysis of ONPG (orthonitrophenylßdgalactoside) can be tested by means of an ONPG Disc. A yellow color indicates a positive reaction. 13. apid urease (Ur) A strong positive urea reaction in the Urea reaction chamber of the Oxi/FermPluriTest at 18 to 24 hours of incubation, indicated by a strong pink coloration is positive. 14. Acid formation from salicin (ali) Certain bacteria produce acids from salicin. Use CTA Medium with alicin for this test and follow the instructions given for this medium. Alternatively use alicin Test according to the procedure reported on the instructions sheet. 15. erology (serol) The identification results Vibrio cholerae and Pseudomonas pseudomallei must be confirmed by serological tests. 16. Beta hemolysis (ßhem) treak the isolate on a Columbia Agar (heep Blood 5%) plate. Incubate at the optimal temperature of the isolate not longer than 48 hours. Clear zones around the colonies indicate beta hemolysis. 17. Growth on TCB Agar (TCB) treak the isolate on a TCB Agar plate. An incubation temperature of 30 to 36 C is optimal. TCB Agar is a selective differential medium for Vibrio species. 18. VogesProskauer eaction (VP) Certain bacterial species produce acetoin (acetyl methyl carbinol) from glucose. The test can be conducted used the VP Test Kit following the procedure reported on the instructions sheet. 19
23 iofilchem Oxi/FermPluriTest Codebook ev.0/ Appendix 3. Availability of media and reagents needed for the performance of the confirmatory tests. Procedure number according to Appendix 2 Name of medium or reagent ef. 1, 9 Tryptic oy Broth Mueller Hinton II Agar CA NO PB TE TOB Catalase Oxy/Test , 5 Tryptic oy Agar Pseudomonas (Cetrimide) Agar DNase Test Agar Nutrient atin MacConkey II Agar Nitrate Broth Nitrate Test ONPG Disc alicin Test Columbia Agar (heep Blood 5%) TCB Agar VP Test Kit
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