Bergen Community College. Spring Lecture Outline/Syllabus. Professor Daneen Aromando, AAS, CVT. Professor Lorena Lago, BS, LVT

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1 Bergen Community College Spring 2013 Lecture Outline/Syllabus Course Title: Instructor: Lab Instructor: Research Animal Technology, VET104 Professor Daneen Aromando, AAS, CVT Dr. Eryn Taormina, DVM Professor Daneen Aromando, AAS, CVT Professor Lorena Lago, BS, LVT Professor Lisa Picht, BS, LVT Professor Harriet Terodemos, MS, CVT, RALAT Program Affiliation: Veterinary Technology Prerequisites: Introduction to Veterinary Technology Course Credits/Hours: 3 Credits/2 Lecture, 3 Lab Attendance-Lecture: Mandatory; Absences should be reported immediately. Missing more than (2) lab sessions and/or (3) lectures will result in an automatic failure. Attendance-Laboratory: Mandatory; Missing more than (2) lab sessions and/or (3) lectures will result in an automatic failure. [Soft Break]Labs cannot be made up. Tardiness: Lateness (> 15 minutes) will count as ½ absence and will result in lowering the lecture portion of the final course grade Class Cancellation: Prior to EVERY class students are urged to check their accounts as well as BCC s home page.

2 Course Description: Research Animal Technology is an introduction to the handling, husbandry, and medical care of common laboratory animals. Lectures will cover the principles and ethics of animal research, as well as the laws that regulate the use of animals to ensure that they are treated humanely. Laboratory sessions will provide hands-on training in restraint, drug administration, sample collection, anesthesia, and research techniques. Course Materials: Required: Sirois, Margi. Laboratory Animal Medicine: Principles and Procedures. St. Louis, MO: Mosby; National Research Council. Guide for the Care and Use of Laboratory Animals. Washington, DC: National Academy Press; 2010 (preferred) or Please note that these documents can be accessed on-line with links provided within the Moodle shell. Supplemental/Recommended References: Ballard, B and Cheek, R. Exotic Animal Medicine for the Veterinary Technician. 2 nd ed. Ames, IA: Wiley-Blackwell; Harkness, JE, Wagner, JE. The Biology and Medicine of Rabbits and Rodents. 4 th ed. Baltimore, MD: Williams & Wilkins; Crow, SE, Walshaw, SO. Manual of Clinical Procedures of the Dog, Cat and Rabbit. 2 nd ed. Philadelphia, PA: Lippincott-Raven; Lawson, PT, ed. Assistant Laboratory Animal Technician (ALAT) Training Manual. Cordova, TN: American Association for Laboratory Animal Science (AALAS); Quesenberry, KE and Carpenter, JW. Ferrets, Rabbits, and Rodents: Clinical Medicine and Surgery. 2 nd ed. St. Louis, MO; Additional Handouts

3 Course Goals: To enable the student to provide complete care for rodents and rabbits kept in conventional laboratory facilities, including the various methods of feeding and watering To reinforce the student s knowledge of nutrition and its relevance to overall laboratory animal health To familiarize the student with the environmental and cage requirements for the different species and the designs and materials available for that purpose To introduce the student to the concepts of sanitation and sterilization To provide the student with an understanding of the animal body s defense mechanisms with an emphasis on immune responses To familiarize the student with the pathophysiology, diagnosis, and control of common diseases and parasites that frequently afflict laboratory animals To introduce the student to the basic concepts of gnotobiotics, including specific pathogen-free animals, barrier-maintained colonies, isolators, and clean rooms To explain safe handling and restraint of common laboratory animals To provide the student with an appreciation of the potential hazards involved in working with animals and the appropriate steps necessary to avoid injury and contracting zoonotic diseases To enable the student to acquire skill in the proper handling of syringes and needles, the calculation of drug dosages, and the administration of medications by both oral and parenteral routes to rodents and rabbits To provide the student with the knowledge to safely induce anesthesia, monitor and recover mice and rats using pentobarbital, xylazine, ketamine, and gas anesthesia To introduce the student to various blood collection techniques and proper handling necessary to provide samples of whole blood or serum To enable the student to make PCV determinations using the microhematocrit method To reinforce the student s knowledge of reproductive anatomy and physiology, with an emphasis on the estrous cycle and mating procedures in common species To provide the student with a knowledge of pseudopregnancy, gestation, parturition, lactation, and weaning as they occur in rodents and rabbits To familiarize the student with the methods used in inbreeding and random breeding of laboratory animals To enable the student to euthanatize animals, perform necropsy prosections, and collect specimens for histopathologic examination To familiarize the student with the various methods used for individual animal identification To introduce the student to administrative and animal records that are kept in most research facilities To provide the student with a knowledge of the legal, ethical, and moral constraints under which research animals are handled

4 Student Learning Objectives: Upon completion of this course, the student will be able to: Describe the caging and nutritional requirements of the commonly used laboratory animals, including the different methods of feeding and watering, giving the advantages and disadvantages of each Explain the basic anatomical and physiological idiosyncrasies of the individual species of laboratory animals List the major diseases affecting different species of laboratory animals and discuss their etiologies, symptoms, diagnosis, and control Explain the principles of cleaning, sanitation, and sterilization and how they relate to conventional and barrier-maintained research animal facilities Describe immune mechanisms and other body defenses against disease Define and differentiate between gnotobiotic, SPF, and barrier maintained animals, and describe methods for their derivation and maintenance Describe the phases of the estrous cycle, mating, pseudopregnancy, gestation, parturition, lactation, and weaning in rodents and rabbits List the different types of breeding programs, the characteristics of inbred, hybrid and random-bred strains; and the conventions to be followed in strain designations Explain the principles of restraint used for non-human primates Explain the state and federal laws governing the use of animals in research and the professional obligations of the veterinary technician to the animals under his/her care Analyze the arguments for and against the use of animals in research and testing Lecture Testing and Grading Criteria: All tests consist only of objective multiple choice type, matching, and fill-in questions. There will be NO make-up exams. Tardiness to exams will NOT be tolerated, therefore, arriving or submitting late to a test forfeits your right to take it.

5 Course Grading Criteria: 50% Lecture 3 Lecture 10% 1 Research paper and presentation 10% 8 Homework assignments, total 5% 1 Species Comparison Chart 5% 50% Lab Husbandry 5% Rat Practicum (Anesthesia) 10% Mouse Practicum 10% Rabbit Practicum 5% Quiz #1, #2 and #3, each worth 2.5% Drug calculations (2), for a total of 2.5% LAB Your lab grade will be based on your performance on homework assignments, animal handling techniques, husbandry skills, and practical examinations. Specific instructions will be discussed during your laboratory session. Animal Husbandry: A portion of your lab grade will be based on your ability to effectively perform animal husbandry duties. These are daily duties and they are MANDATORY. Each student will choose 7 days over the course of the semester where they will come to school and take care of the rodents or rabbits. This includes the daily maintenance of their living environment, providing feed and clean water, and performing physical examinations, as well as ensuring that environmental conditions in the vivarium are suitable for the proper health and wellbeing of the animals. If you are unable to make your assigned shift, you must find coverage. If you do not show up for your assigned shift, you may fail the class. Contact Information: Should you have any course related questions, please contact your instructor via . If there is an animal related question, please call, in the following order: Professor Terodemos hterodemos@bergen.edu Professor Aromando daromando@bergen.edu Professor Picht lpicht@bergen.edu

6 Essential Tasks: All students are required to perform the following tasks, which will be signed off on once each task is completed. Each student will be given a task booklet for this. All techniques must be performed properly and proficiently in order to pass the lab portion of this course. Recognize and restrain rodents and rabbits Determine the sex of rodents and rabbits; understand reproduction in same species Handle rodents and rabbits Understand the nutritional needs for rodents, rabbits, and various species Provide basic husbandry tasks for rodents and rabbits, including feed and water Understand and utilize appropriate methods of identification for rodents and rabbits Perform Subcutaneous injection, rodents and rabbits Perform Intramuscular injection, rodents and rabbits Perform Intraperitoneal injection, rodents Perform Intravenous injection, rodents Collect blood samples (IV, Retro-orbital) rodents and rabbits Perform Oral Dosing (Gavage), rodents Clean and medicate ears, rabbits Perform necropsy and collect specimens Anesthetize rats, monitor vital signs (HR, RR), and perform recovery procedures Explain common disease signs, rodents and rabbits Understand vectors, fomites Understand disinfectants, antiseptics, and sterilization In addition, we will: Discuss the pharmaceutical environment. Review the government agencies that guide and direct a pharmaceutical world, such as the FDA, USDA, NIH, AALAS, and IACUC. We will cover experimental design and animal husbandry. We will discuss the proper way to handle paperwork within the animal care laboratory. We will cover blood sampling and observe alternate methods of blood sampling and appropriate sampling tubes. We will also discuss the humane ways in which animals are euthanized. Textbook There is no textbook for the lab portion of the course. Handouts may be issued periodically. Laboratory Conduct: Students are required to wear scrubs or lab coats to lab. NO Exceptions. Students are responsible to clean up their work areas after their work is completed. All supplies and materials should be put in their place, and your workstations should be cleaned and disinfected. If a student is bit, scratched, or sustains a needle stick injury, this must be reported to the instructor immediately, and first aid will be provided, followed by a visit to the school nurse. You will be required to fill out an injury report. Cell phone usage is not permitted in lab. Photos are not permitted to be taken under any circumstances. Tardiness is not acceptable. If you are late, it may be considered as half of an absence.

7 Lecture Topic Outline: Overview of the field of laboratory animal science, including the history and development of animal technology, scientific organizations affiliated with the field, legislation controlling the use of animals, members of the research team, and the ethics of animal use Animal health and disease: pathology, etiology, and clinical symptomatology Drug therapy, pharmacology, and euthanasia The mouse and the rat: anatomical idiosyncrasies, behavior, husbandry, handling, restraint, and common diseases The laboratory animal environment: Facility design requirements and potential biohazards Facility equipment and sanitation, hygiene and safety in the laboratory animal facility Feed and nutrition Rabbits: anatomical idiosyncrasies, behavior, husbandry, handling, restraint, and common diseases Animal procurement, transportation, and quarantine Genetics and breeding of laboratory animals and methods of individual animal identification Surgical and research techniques, experimental design, and methodology Hamsters, guinea pigs, and other rodents: anatomical idiosyncrasies, behavior, husbandry, handling, restraint, and common diseases Cats and dogs: behavior, husbandry, handling, restraint and common diseases Nonhuman primates: behavior, husbandry, handling, restraint and common diseases State and federal laws governing the care and use of laboratory animals

8 Research Animal Technology Lecture Schedule This is schedule will be modified to fit the semester. Week Sirois Lecture Topic Homework Due Exam Readings 1 Chapter 1 Intro to Lab Animal Medicine Ch 1 Study Questions, pp 25 & 26 2 Chapter 2 The Research Environment Ch 2 Study Questions, pg 51 3 Chapter 3 The Rat Ch 3 Study Questions, pg 85 4 Finish The Rat 5 Chapter 4 The Mouse Ch 4 Study Questions, pg 114 Guide Exam Opens 6 Finish The Mouse Guide Exam Closes 7 Chapter 7 The Rabbit Ch 7 Study Questions, pg Assign Nontraditional Presentations Midterm Exam (Ch 1-4) Opens 9 Chapter 5 The Guinea Pig Ch 5 Study Questions, pg 138 Midterm Exam Closes 10 Chapter 6 The Hamster and The Gerbil Ch 6 Study Questions, pg The Ferret and the Chinchilla 12 Chapter 8 NonHuman Primates Ch 8 Study Questions, pg 220 Nontrad Chart Info Due 13 Nontraditional Presentations Final Exam Opens Cumulative with Emphasis 7, 5, 6, 8 and Nontraditional Species 14 Nontraditional Presentations Species Chart Due Final Exam Closes 15 Nontraditional Presentations

9 Research Animal Technology Laboratory Schedule Week Topic Assignment Exam 1 Introduction to Lab Animal Medicine; The Research Environment; Sanitation; IACUC, Provide complete contact information; Sign-up for Animal Husbandry Shifts Protocols, GLP s and SOP s 2 BCC Vivarium SOP and Set up; Quiz One Permanent/Nonpermanent Identification; Rat Husbandry 3 Injection Techniques (Model); Quiz Two Rat Restraint 4 Gavage and Injections (SQ, IM, IV) Rat 5 Gavage and Injections Rat; Drug Dose Calculations Due 6 Rat Practicum Additional Pharmacy Calculations Due; Rat Practicum Bring Anesthesia Monitoring Sheet to Lab 7 Mouse Husbandry and Restraint; Injection Techniques (Model); Humane Euthanasia 8 Gavage and Injections (SQ, IM, IV) Mouse 9 Gavage and Injections Mouse 10 Mouse Practicum Mouse Practicum 11 Rabbit Husbandry and Restraint; Injection Techniques (Model); Rabbit Intubation Video 12 Rabbit Injections and Venipuncture Quiz Three 13 Rabbit Practicum Rabbit Practicum (in lab); Lab Final Opens (online) 14 Clean BCC Vivarium Lab Final Closes 15 Reset BCC Vivarium

10 Americans with Disabilities Act Students who require accommodations in accordance with the Americans with Disabilities Act can request these services from the Office of Specialized Services. To learn more about how to apply for these services, please visit them at: Student and Faculty Support Services The Distance Learning Office for any problems you may have accessing your online courses Smarthinking Tutorial Service Room C- 334 On Line at: The Tutoring Center Room L- 125 The Writing Center Room L The Online Writing Lab (OWL) The Office of Specialized Services (for Students with Disabilities) The Sidney Silverman Library Reference Desk On Line at: Room Room L

11 Important College Policies Found within the College Policy Manual at Withdrawal from Classes and Refunds, pp Grading, pp Course Grade Appeal Policy, p. 54. Academic Integrity and Plagiarism, pp [Read this carefully.] Class Attendance Policy, pp Acceptable Use of Information Technology Resources, pp Clubs, pp Code of Student Conduct or Standards of Conduct, pp Alcohol and Drug Policy, pp Family Education Rights and Privacy Act of 1974, p. 79. ADA and Grievance Procedures p. 107 Sexual Harassment Policy, pp Campus Assault Victim's Bill of Rights, pp Smoking Policy, pp Traffic Regulations, p. 77.