Joint Meeting of the 51 st Livestock Insect Workers Conference and the 9 th International Symposium on Ectoparasites of Pets

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3 Joint Meeting of the 51 st Livestock Insect Workers Conference and the 9 th International Symposium on Ectoparasites of Pets 11:00-4:00 Registration -- Patterson Registration Booth Program Sunday - 10 June :30-1:45 Welcome and Introductions -- Fred Knapp & John Webb Patterson Rooms C & D 1:45-2:15 Overview of Kentucky and the Horse Industry Keynote Speaker: Peter Timoney Moderator: Miscellaneous submissions Phil Kaufman 2:15-2:30 Evaluation of a stable fly trap in a Nebraska pasture John B. Campbell and D.J. Boxler 2:30-2:45 Comparison of trap efficacy to enhance management of horse, deer and stable flies affecting dairy animals on northeast US pastures. J. Keith Waldron, P.E. Kaufman, C. Strong and D.A. Rutz 2:45-3:00 An attractant for house flies based on components identified in blackstrap molasses Christopher J. Geden, B.A. Quinn, U.R. Bernier and J.A. Hogsette 3:00-3:30 Break Moderator: Insecticide and Acaricide Resistance Alec Gerry 3:30-3:50 Pesticide Resistance: Challenges and Future Directions Keynote Speaker: Michael Rust 3:50-4:05 Efficacy of amitraz applied as a single dip treatment against an amitraz-resistant strain of Boophilus microplus (Acari: Ixodidae) infested on cattle Ronald Davey, R.J. Miller, and J.E. George 4:05-4:20 Lesser mealworm research in Australia: How far have we come? Trevor A. Lambkin, S.J. Rice, J.S. Bartlett and T.M. Lambkin 4:20-4:35 Efficacy against fleas, ticks and mosquitoes of some registration-exempt pesticide products on dogs and cats Thomas Miller, M. Sharp, L. Nouvel 4:35-4:50 Horn fly suppression: Field comparison of Avenger (endosulfan) and Warrior (diazinon-chlorpyrifos) ear tags Nancy C. Hinkle, T.W. Wilson, and P.C. Worley 4:50-5:05 Susceptibility of lesser mealworm (Coleoptera: Tenebrionidae) adults and larvae exposed to two commercial insecticides on unpainted plywood panels Phillip E. Kaufman, D.A. Rutz and C. Strong 5:05-5:20 Efficacy of four registered spot-on products against fleas and ticks on dogs T. Miller, M. Sharp, L. Nouvel 5:20-5:30 Wrap-Up/Announcements - Fred Knapp 6:00-7:00 Welcome Reception: Light refreshments: - Regency Kindly supported by 7:30 Dinner on your own. Reservations at local restaurants are suggested. 5

4 Monday - 11 June :00-8:00 Breakfast for all Registrants: Patterson Rooms A & B 7:00-5:00 Registration -- Patterson Registration Booth 8:00-4:45 Poster Session and Exhibits: Patterson Rooms E - G 8:00-8:10 Announcements -- Fred Knapp & John Webb Patterson Rooms C & D 8:10-8:25 Welcome to Kentucky: Dr. M. Scott Smith, Dean, College of Agriculture, University of Kentucky 8:25-8:40 Welcome to Lexington: Dr. John Obrycki, Chair, Department of Entomology, University of Kentucky 8:40-9:10 The USDA Agricultural Research Service s National Program 104: Veterinary, Medical, and Urban Entomology Keynote Speaker: Daniel Strickman Insecticide and Acaricide Resistance Moderator: Christian Epe 9:10-9:25 Knockdown and residual efficacy of gammacyhalothrin pour-on formulations against experimental and natural horn fly infestations on cattle Christine M. McCoy, W.H. White, J. Meyer, K.A. Meyer, K.A. Baker, D.E. Snyder, J.R. Winkle, E. Ahrens, R.G. Pennington, C.R. Pennington, D.G. Wujek 9:25-9:40 Efficacy testing against Stomoxys calcitrans on horses under controlled environmental conditions Lori Carter and T. Hartwell 9:40-9:55 Resistance of horn fly Haematobia irritans (Diptera: Muscidae), to insecticides in Colombia: Present situation Efraín O. Benavides and R.Q. Bonilla 9:55-10:10 Management of organophosphate resistant horn flies using a mid-season avermectin treatment of cattle in Louisiana Cole Younger 10:10-10:40 Break Kindly supported by Moderator: Ectoparasiticide Discovery Catherine Caulfield 10:40-11:00 Chance and Design in Insecticide Discovery Keynote Speaker: Vincent Salgado 11:15-11:30 Dinotefuran: a novel third-generation neonicotinoid providing fast flea adulticidal efficacy on dogs Albert Ahn 11:30-11:45 Efficacy of the combination fipronil/(s)-methoprene (FRONTLINE Plus) against flea eggs and larvae (Ctenocephalides felis) following weekly infestations of treated cats with gravid female fleas Michel Franc and F. Beugnet 11:45-12:00 Doramectin-medicated molasses for control of horn flies J. Allen Miller, K.H. Lohmeyer and J.M. Pound 12:00-1:30 Lunch: Regency Kindly supported by 1:30-1:45 Dinotefuran: a novel third-generation neonicotinoid with ideal characteristics for fast-acting long term flea control in cats Albert Ahn 1:45-2:00 Efficacy of Advantage Multi (imidacloprid + moxidectin) topical solution for the treatment of flea (Ctenocephalides felis ) infestation on cats. Robert G. Arther, O. Hansen and D.R. Young 2:00-2:15 Evaluation of the attractiveness of several novel octenol analogs to adult southern house mosquitoes (Culex quinquefasciatus) James E. Cilek and C.O. Ikediobi 2:15-2:30 Discussion 6

5 Moderator Miscellaneous submissions John Webb 2:30-2:45 Immunization studies in rabbits using gut membrane-bound proteins derived from Rhipicephalus appendiculatus, R. evertsi evertsi and Amblyomma variegatum female ticks Helen L. Kutima, O. Amoo, M. Imbuga and P.M. Kutima 2:45-3:00 Co-Ral Plus ear tags to control horn fly, Haematobia irritans (L.), in eastern Montana. Gregory Johnson 3:00-3:30 Break Kindly supported by 3:30-3:45 Field survey of pest Diptera and beneficial Coleoptera associated with beef cattle in Washington State Holly Ferguson, R. Zack, S. O Neal Coates, C. White and D. Walsh 3:45-4:00 The effect of behavioral conditioning on determining host choice in a parasitoid of filth flies, Trichopria nigra Kimberly M. Ferrero and C.J. Geden 4:00-4:45 Merial Mini-Symposium Efficacy of fipronil against Ctenocephalides felis and the relationship with the Rdl gene Albert Boeckh 4:45-5:20 Preliminary Business Meetings Patterson Rooms C & D 5:20-5:30 Wrap-Up/Announcements - Fred Knapp 6:30 Dinner: Location TBD Kindly supported by Merial Public Relations and Mosquito Control (and other humorous things) David Brown, Manager of the California Sacramento-Yolo Mosquito and Vector Control Division. 7

6 Tuesday - 12 June :00-8:00 Breakfast for all Registrants: Patterson Rooms A & B 7:00-12:00 Registration -- Patterson Registration Booth 8:00-1:30 Poster Session and Exhibits: Patterson Rooms E - G 8:00-8:10 Announcements -- Fred Knapp & John Webb Patterson Rooms C & D Moderator Parasitic Arthropods, Biosecurity and Food Safety Glen Scoles 8:10-8:30 Beyond Overt Biological Terrorism: Fleas, Flies, Ticks and Zoonoses and the Threats They Pose Keynote Speaker: Steven Presley Age distribution and amount of (Z)-9-tricosene on wild house flies collected from dairies 8:30-8:45 Sarah M. Butler, R.D. Moon, N.C. Hinkle, J.G. Millar, J.S. McElfresh and B.A. Mullens 8:45-9:00 Efficacy of chemical attractants on Tabanidae collections with Pacific blue Nzi traps Daniel L. Kline and S.A. Allan 9:00-9:15 Human waste (biosolids): Fly dreaming, people nightmare Ian Dadour and N. Penney 9:15-9:30 Feeding dynamics of stable flies: The consequences of inflicting painful bites Alberto B. Broce and J.A. Hogsette 9:30-9:45 Evaluation of a walk through stable fly control device David J. Boxler and J.B. Campbell 9:45-10:00 Role of conspecific eggs and associated bacterial community in oviposition behavior of stable flies, Stomoxys calcitrans L. Ludek Zurek, F. Mramba and A. Broce 10:00-10:30 Break Kindly supported by Moderator: Industry Session Bill Donahue 10:30-12:00 Open session 12:00-1:30 Lunch: Regency Kindly supported by 1:30 Free Time: Plan your own activities 6:00 Dinner on your own. Reservations at local restaurants are suggested. 8

7 Wednesday - 13 June :00-8:00 Breakfast for all Registrants: Patterson Rooms A & B 7:00-4:00 Registration -- Patterson Registration Booth 8:00-4:00 Poster Session and Exhibits: Patterson Rooms E - G 8:00-8:10 Announcements -- Fred Knapp & John Webb Patterson Rooms C & D Moderator Veterinary Medicine and Ectoparasitism Nancy Hinkle 8:10-8:30 Status of Veterinary Entomology in Europe Keynote Speaker: Christian Epe 8:30-8:45 Comparison of a horse and a carbon dioxide-baited suction trap for collecting hematophagous Diptera in southern California Alec C. Gerry, T.M. Nawaey, P.B. Sanghrajka, J. Wisneiwska and P. Hullinger 8:45-9:00 Fall equine abortion associated with exposure to walnut caterpillars, Datana integerrima (Lepidoptera: Notodontidae) in Pasco County Florida John Roberts, J. Ward, J. Foltz, P. Kaufman, C. Daniels, J. Donahue, C. Schuman and L. Harrison 9:00-9:15 Detection and quantification of an insect repellent in bovine milk D. Wes Watson, M.A. Drake, S. Washburn and J.F. Butler 9:15-9:30 Spatial distribution of African sheep louse, Linognathus africanus Kellogg & Paine, on sheep John E. Lloyd, G.D. Johnson, R.W. Kott, H.B. Goosey and D.E. Legg 9:30-9:45 Discussion 9:45-10:15 Break Kindly supported by 10:15-10:35 Status of Veterinary Entomology in North America Keynote Speaker: Ann Donoghue Kindly supported by 10:35-10:50 Potential role of domestic animals in maintaining bed bug infestations in human environments Alvaro Romero and M.F. Potter 10:50-11:05 Clean-up Pour-on insecticide with IGR to control sheep ked, Melophagus ovinus (L.), and African sheep louse, Linognathus africanus Kellogg & Paine Gregory D. Johnson, J.E. Lloyd, R.W. Kott, H.B. Goosey and D.E. Legg 11:05-11:20 House fly management in arid environments Jerome A. Hogsette, D.F. Hoel and U.R. Bernier 11:20-11:35 Efficacy of Elector PSP (spinosad) for the control of the lesser mealworm (Alphitobius diaperinus Panzer) in heavy broilers S. Mike Stringham, D.W. Watson, S. Denning, K. Baker and G. Balme 11:35-12:00 Discussion 12:00-1:30 Lunch: Regency 1:30-3:00 Bayer Mini-symposium Moderator Norbert Mencke 1:30-1:50 The development and efficacy of QuickBayt Spot Spray (imidacloprid) for house fly control in food animal premises Douglas H. Ross 1:50-2:10 Evaluation of the efficacy of two imidacloprid bait formulations for the control of naturally occurring infestations of house flies, Musca domestica in three central California dairies William Donahue 2:10-2:35 Movement of Rhipicephalus sanguineus adults between co-housed dogs during active feeding Susan E. Little, J. Hostetler and K.M. Kocan 2:35-3:00 Canine Leishmaniosis in an endemic area in Southern Italy and the efficacy of imidacloprid/ permethrin. Cinzia Cantacessi, D. Otranto, P. Paradies, R. Paolo Lia, G. Capelli, D. Stanneck and N. Mencke 3:00-3:10 Announcements -- Fred Knapp & John Webb 3:10-3:40 Break 3:40-4:30 Final Business Meetings Patterson Rooms C & D 7:00-10:00 LIWC-ISEP Kentucky Horse Park 9 Kindly supported by Kindly supported by

8 Thursday - 14 June :00-8:00 Breakfast for all Registrants: Patterson Rooms A & B 7:00-9:00 Registration -- Patterson Registration Booth 8:00-12:00 Poster Session and Exhibits: Patterson Rooms E - G 8:00-8:10 Announcements -- Fred Knapp & John Webb Patterson Rooms C & D Moderator Epidemiology: Global Warming, New and Emerging Diseases Adalberto Pérez de León 8:10-8:30 Global Change Forces and Climate -- Things to Think About Keynote Speaker: Allan Nelson Update on the development of a Geographic Information System (GIS) graphical diagnostic database for 8:30-8:45 investigation of Boophilus spp. tick infestations in Zapata County, Texas Diane M. Kammlah, J.M. Pound, J.E. George, R.B. Davey, R. Duhaime and E.J. Bowers 8:45-9:00 Persistent transovarial transmission of Babesia caballi by the tropical horse tick, Dermacentor nitens, reared on a non-susceptible host O. Nicolas Schwint, L.S. Kappmeyer, D.P. Knowles and G.A. Scoles 9:00-9:15 Identification of bacterial pathogens in ticks collected from NW Arkansas canines Rebecca T. Trout, C.D. Steelman and A.L. Szalanski 9:15-9:30 White-tailed deer and other wild ungulates Their influence on success of the Fever Tick Eradication Program J. Mathews Pound, J.A. Miller, J.E. George, D.M. Kammlah and R. Duhaime 9:30-9:45 Blacklegged ticks (Ixodes scapularis) and their associated pathogens collected from white-tailed deer in eastern Tennessee Graham J. Hickling, Reid R. Gerhardt and C.J. Jones 9:45-10:00 Persistence of inoculated Salmonella in adult darkling beetles, Alphitobius diaperinus (Coleoptera: Tenebrionidae) Aubree J. Roche, L.J. Richardson, N.A. Cox, N.C. Hinkle, R.J. Buhr and G.R. Siragusa 10:00-10:30 Break Kindly supported by and 10:30-10:45 Flies and Exotic Newcastle disease virus (ENDV) Seemanti Chakrabarti, D.J. King, C. Cardona, D.E. Swayne, D. Kuney and A.C. Gerry 10:45-11:00 READY FOR A DISASTER? Lori Carter and B. Sabol 11:00-11:15 Co-transmission of multiple Ehrlichia spp. between white-tailed deer (Odocoileus virginianus) by lone star ticks (Amblyomma americanum). Susan E. Little, L.M. Parsons, M. West and M.J. Yabsley 11:15-11:45 Meeting Summary: Bill Donahue 11:45-12:00 Final comments: Fred Knapp 12:00 Departure: Have a safe trip home! 10

9 WELCOME Welcome to the first joint meeting of the 51st Livestock Insect Workers Conference (LIWC) and the 9 th Biennial International Symposium of Ectoparasites of Pets (ISEP). The LIWC first started as a meeting at the USDA/ARS laboratory in Kerrville, Texas in an effort to speed up the development of the first animal systemic insecticide for control of the cattle grub. Since then the LIWC has broadened its horizon to cover all livestock insect pests. ISEP was established in 1991 to address the biology and control of ectoparasites of pets and companion animals, and the first and second meeting was held here in Lexington in the same hotel as this meeting. It was not until the 2 nd meeting that International was added to the name of the Symposium. Lexington, Kentucky is known as the Horse Capital of the World and is the home to the University of Kentucky. The city of Lexington was established in 1775 and is often called the northernmost Southern City. Lexington is centrally located in the Blue Grass region at the crossroads of I-75 and I-64; a one day s drive for 75% of the population in the USA.. Some of our wealthiest residents are horses that can be seen on over 450 horse farms within the area. Frequent guests to Kentucky include counts, sheiks and monarchs. Queen Elizabeth II visited here a few weeks ago. Many horse events are held at the 1200 acre Kentucky horse park. The three day Rolex event is held each April and in 2010, the Alltech FEI World Equestrian Games will be held at the park. Each spring and fall powerful Thoroughbreds capture the hearts of racing fans at the famous Keeneland race course; a thoroughbred legacy. The track started in 1916 and in 1936, after some hard time during the depression, opened to the public as a non profit association. Besides racing, Keeneland is the international leader in Thoroughbred auction sales. Lexington is also home to the oldest harness racing track in the region. It dates back to 1875 and is located in the center of the city. Kentucky is full of history and we invite you to see the variety of equine attractions, scenic beauty, the bluegrass heritage historic area, the creative crafts, museums and more. For those of you that have a thirst for knowledge, visit our famous distilleries. More than 200 years ago, bourbon was born in Kentucky and today more than 95% of bourbon is produced in the state. Be sure to try one of Kentucky s famous mint Julep during your stay; there is nothing more refreshing on a hot summer day. No matter how you choose to experience Lexington and the Bluegrass Region, we hope that you will have a successful meeting and that you leave us with wonderful memories of our horses, history and hospitality. Y all come back. Fred Knapp Emeritus Professor of Entomology College of Agriculture University of Kentucky 11

10 Program Committee Fred Knapp Department of Entomology S-225 Agriculture Science Bldg. N. University of Kentucky Lexington, KY office home John & Leslie Webb Elkin Station Inc. 655 Elkin Station Road Winchester, KY Phillip Kaufman Entomology & Nematology Dept. University of Florida Gainesville, FL x 159 Mike Stringham, Dept. of Entomology, Box 7626 N.C. State University Raleigh, NC Adalberto Pérez de León MexFra Consulting 716 Sarratt Ridge Court Wake Forest, NC William Donahue, Jr. Sierra Research Laboratories 5100 Parker Road Modesto, California Christian Epe Hannover Univ. of Veterinary Medicine Department for Infectious Diseases Institute for Parasitology Buenteweg 17; D Hannover Norbert Mencke Bayer HealthCare AG, Animal Health Global Marketing - Veterinary Services D Leverkusen, Germany Tel.: +49 (0) Nancy Hinkle Department of Entomology University of Georgia Athens GA Karissa Arnold Department of Entomology University of Kentucky S-225 Agriculture Science Center North Lexington, KY

11 We kindly thank the following sponsors of the 2007 LIWC-ISEP meeting. 13

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13 Poster Session Authors Kelly Allen, M.V. Reichard, E.M. Johnson and S.E. Little Alberto B. Broce, J. Kalisch and D. Keith Aly Chapman, K. Claudia and P. Sharon Kelly Loftin, J. Pennington, S. Brazill, D. Steelman, A. Szalanski, K. VanDevender, T. McKay, W. Watson, S. Willard and J. Edwards Lindsey M. Parsons and S.E. Little Elena Popa and A. Hiottu Donald A. Rutz, P.E. Kaufman, C. Strong and J.K. Waldron James L. Alexander and G. Schuster Sally O Neal Coates, C.J. White, H.J. Ferguson, D.B. Walsh Title Prevalence and diversity of Hepatozoon spp. in naturally-infected dogs from Oklahoma Pyemotes herfsi, a species of itch mites new to North America as the cause of bite outbreaks on humans and pets Centipede (Class Chilopoda) envenomation in a hypersensitive dog Using parasitic wasps as an IPM approach to manage filth flies in southern dairies Engorgement and reproductive rates of Amblyomma americanum fed on antibiotic-treated rabbits Ectoparasite species causing acariosis or dermatites in companion animals in Romania A self-contained automated sprayer system for control of flies on pastured dairy cattle Current status and review of the screwworm Reduced risk pest management strategies in beef cattle 15

14 Category Oral Presentation Poster Display Dog, tick X Title: Author (s): Affiliation: Prevalence and diversity of Hepatozoon spp. in naturally-infected dogs from Oklahoma. Kelly E. Allen, Mason V. Reichard, Eileen M. Johnson, and Susan E. Little. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK. Abstract: Hepatozoon americanum is a tick-borne protozoan that causes American Canine Hepatozoonosis (ACH) in the southern United States; the pathogen is transmitted to dogs via ingestion of infected Amblyomma maculatum ticks. To determine the prevalence and phylogenetic diversity of H. americanum in domestic dogs from Oklahoma, blood samples collected from shelter dogs, dogs residing in endemic areas of the state, and suspected clinical cases of ACH presenting to veterinarians were evaluated by PCR of the 18S rrna gene of Hepatozoon spp. Hepatozoon sequence recovered from dogs from areas where ACH is endemic and those from clinically-ill dogs most closely resembled H. americanum previously reported from dogs in Alabama and Oklahoma. Testing blood from shelter dogs revealed evidence of Hepatozoon spp. in three of 200 (1.5%) samples assayed. Surprisingly, sequence analysis revealed only one of these amplicons closely resembled existing sequence data for H. americanum; the remaining two sequences were most similar to sequence of Hepatozoon canis, a pathogen of dogs that has not been previously reported in the United States. In sum, we documented a surprising degree of diversity in the 18S rrna gene fragment of the Hepatozoon in naturally-infected domestic dogs, suggesting that multiple strains or species of Hepatozoon may be able to infect and cause disease in dogs in North America. Additional research is needed to confirm this apparent diversity of species and to further evaluate the identity of Hepatozoon spp. cycling in dogs, ticks, and wildlife. 16

15 Commodity: Pets Poster Display X Title: Pyemotes herfsi, a species of itch mites new to North America as the cause of bite outbreaks on humans and pets Authors: Alberto B. Broce 1, Jim Kalisch 2 and Dave Keith 2 Affiliation: 1 Department of Entomology, Kansas State University, Manhattan, KS 2 Department of Entomology, University of Nebraska, Lincoln, NE Abstract: Outbreaks of itching bites in SE Kansas and Lincoln, Nebraska in the summer of 2004 led to the discovery of Pyemotes herfsi (Acari: Pyemotidae) an introduced species of European origin. Typical bites are red welts with a vesicle in the center; itching but painful when scratched. Although the mites presence is readily felt by people, pets can also feel the attack of these mites. They develop by preying on the larvae of a Contarinia sp. of gall-making midges on oak leaves. The mites great reproductive potential, small size and dispersal by wind make them difficult to control. Although we have obtained substantial information about this mites biology; we lack information about their whereabouts from November to July. Their presence has been already recorded over a large area in the Midwest USA, from Kansas to Tennessee, and from Nebraska to Texas. 17

16 Category Oral Presentation Poster Display X Title: Author (s): Affiliation: Centipede (Class Chilopoda) Envenomation in a Hypersensitive Dog Chapman Aly, Kirk Claudia, and Patton Sharon University of Tennessee College of Veterinary Medicine, 2407 River Drive, Knoxville, TN Abstract: There are at least 3,000 species of centipedes (Class Chilopoda) worldwide that belong to the four orders Scutigeromorpha, Lithiobiomorpha, Scolopendromorpha, and Geophilomorpha. The more venomous members are within the order Scolopendromorpha. Scolopendra spp venom contains serotonin, histamines, and cardiodepressant factors. In this report, we present the case of a 13 year old, female spayed West Highland White Terrier with sudden onset diarrhea, vomiting, and a firm, approximately 2 cm intradermal lesion on the right lateral flank that became pruritic. The dog had a history of hypersensitivity to antibiotics and other medications, severe food allergies, and severe atopy. The owner submitted a 4.5 cm long centipede that was found in the dog bedding. The centipede was identified as a member of the family Scolopendridae (order Scolopendra). We speculate the centipede bite caused the clinical signs in this atopic dog. Although, to our knowledge, there are no reports of adverse effects from centipede bites in companion animals, there are many reports of sensitivity and allergic reactions in humans ranging from localized swelling and pain to myocardial infarction and rarely death. 18

17 Category Oral Presentation Poster Display X Title: Author (s): Affiliation: Using Parasitic Wasps as an IPM Approach to Manage Filth Flies in Southern Dairies Kelly Loftin1, Jodie Pennington1, Sheri Brazil1, Dayton Steelman1, Allen Szalanski1, Karl VanDevender1, Tanja McKay2, Wes Watson3, Scott Willard4 and Justin Edwards4 1. University of Arkansas, Fayetteville, Arkansas 2. Arkansas State University, Jonesboro, Arkansas 3. North Carolina State University, Raleigh, North Carolina 4. Mississippi State University, Mississippi State, Mississippi Abstract: Producer interest in incorporating commercial releases of pteromalid parasitoids (Hymenoptera: Pteromalidae) into their Musca domestica and Stomoxys calcitrans dairy IPM programs has increased. This renewed interest is partially the result of increased milk premiums for organically produced milk and insecticide resistance. Research based recommendations for using ptermalid wasps is available some regions of the U.S. however research pertaining to southern regions is more limited. In efforts to provide better recommendations for using pteromalid wasps in southern dairies, the southern region SARE (Sustainable Agriculture Research and Education) sponsored a research and education project. During 2005, parasitoid wasps were collected from Arkansas, North Carolina and Mississippi to determine the abundance and species composition of parasitoids in the dairy ecosystem. Ten species of parasitoid wasps in the family Pteromalidae, one in the family Chalcidae and one in the family Ichneumonidae were identified from dairies in Seasonal abundance of the most common pteromalid wasps will also be presented. In addition, preliminary data from 2006 on percentage of house fly parasitism following inundative parasitoid releases will be presented. 19

18 Category Oral Presentation Poster Display X Title: Author (s): Affiliation: Engorgement and reproductive rates of Amblyomma americanum fed on antibiotic-treated rabbits. Lindsey M. Parsons and Susan E. Little. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK. Abstract: To determine if antibiotic-facilitated clearance of rickettsia may influence tick feeding and reproduction, we conducted a preliminary trial in which wild-caught A. americanum (n=50) were fed on either untreated rabbits (n=2) or rabbits treated with 10 mg/kg doxycycline (PO q 24hrs; n=2). Ticks were allowed to feed for 11 days, and then were collected, weighed, and the females held at 90% RH for ovipositing. Each egg mass was weighed, allowed to hatch, and larval numbers determined. Female A. americanum that engorged on antibiotic-treated rabbits had higher average engorgement weights ( / mg) and subsequently produced more larvae on average (3307) than those fed on control rabbits (195 +/ mg, 2913 larvae, respectively), although the small sample size in this pilot trial precluded statistical evidence of this effect (p-values >0.05). Surprisingly, female A. americanum that fed on the left side of each rabbit had significantly higher average engorgement weights ( /- 59 mg) than those fed on the right side ( / mg, p = ), and produced more larvae on average (3235 versus 2840, p =0.0615). Clearance of rickettsia in the ticks which fed on antibiotictreated rabbits may have facilitated tick engorgement and supported reproduction, although the study will need to be repeated with additional animals to more fully evaluate this effect. The dramatic influence of side on engorgement was surprising; we hypothesize that engorgement of ticks fed on the left side may have been facilitated by higher rates of blood flow and/or elevated temperatures. 20

19 NOT FOR PUBLICATION Category Oral Presentation Poster Display Fly X Title: Ectoparasite Species Causeing Acariosis or Dermatites in Companion Animals in Romania Author (s): Elena Popa, Anisoara Hiottu Affiliation: Institut for Diagnosis and Animal Health, ROMANIA Abstract: The underclass Arachnida includes the orders Astigmata, Prostigmata and Metastigmata. The order Astigmata is represented by the Sarcoptidae and Psoroptidae families and contains parasites of cats and dogs. The order Prostigmata is represented by the family Demodecidae and contains parasites of dogs. The order Metastigmata includes the Ixodes and Rhipicephalus (Boophilus) ectoparasites at dogs and cats. The study was conducted from and included 54 companion animals (40 dogs and 14 cats) from 4 districts ( Buzau, Sectorul Agricol Ilfov, Prahova) and Bucharest. Twenty seven dogs and 10 cats were diagnosed with acariosis or dermetites produced by acarians. Six acarid species (Sarcoptes scabiei var. canis, Otodectes cynotis var canis, Sarcoptes scabiei var. cati, Demodex canis, Ixodes ricinus, Rhipicephalus (Boophilus) sanguineus) belonging to Astigmata, Prostigmata and Metastigmata orders were identified. From these species, four caused acariosis diseases (sarcoptosis, otodectosis, demodicosis) and the two Metastigmata species caused dermatites in 8 dogs and 7 cats. Among the 27 dogs with acariosis, 23 were identified as Demodex species (85.18%). Two of the 14 cats (14.28%) presented dermatites and FIV infection. The serological test for felin immunodeficiency virus (FIV) was also positive. The most dogs and cats infestations occurred in Spring when the vegetation and temperature are convenient for acarian development. 21

20 Category Oral Presentation Poster Display Pesticide X Title: A Self-Contained Automated Sprayer System for Control of Flies on Pastured Dairy Cattle Author (s): Affiliation: Donald A. Rutz, 2 Phillip E. Kaufman, Colleen Strong and J. Keith Waldron Dept. of Entomology, Cornell University, Ithaca, NY and 2 Entomology and Nematology Department, University of Florida, Gainesville, FL Abstract: Face flies and stable flies are notoriously difficult to control with insecticides on pastured dairy cattle because of their feeding location on the animal and their intermittent visitation to animals. Furthermore, stable flies are particularly difficult to control on pastured animals with the available conventional insecticide application methods because all of those methods (except whole animal sprays) deliver the insecticide to the animal s upper body regions. A self-contained, solar powered, animal-triggered sprayer has been developed (Kingston Biocontrol). An electronic eye activates the sprayer as the cattle pass through a narrow opening or gate on their way to pasture after milking. The sprayer nozzles are positioned to apply a small, targeted treatment to the animal s face, back and legs. This sprayer system has two very important benefits: (1) a minimal volume of insecticide that is (2) automatically applied to highly effective areas on the animal. This sprayer system was evaluated on central NY dairy farms during 2003 and 2004 for face fly control. In 2005 the sprayer was modified to include stable fly control. During all three years of the study, we observed an overall reduction of fly numbers on the sprayed herds compared to non-sprayed herds. In addition, our collaborating dairy farmers commented on the improved behavioral change that they regularly observed with their cattle as a result of reduced fly annoyance. 22

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