Laboratory Hazard Assessment Tool

Transcription

1 This Laboratory Hazard Assessment Tool (LHAT) facilitates identification of hazards and identifies the Personal Protective Equipment () to be used during the specified work activities. The LHAT must be updated as hazards and personnel change, and at least once every 12 months, irrespective of changes to hazards or personnel. The LHAT will provide a summary report of hazards present in the laboratory and the recommended for laboratory workers. Objectives for the Principal Investigator (PI)*: Identify hazards that are present in the lab Communicate lab hazards to personnel Identify the to be used based on the hazard assessment Provide training to lab personnel Maintain records of assessment and training Objectives for Lab Personnel: Receive information about hazards present in the lab Receive information about to be used to work in the lab Receive and demonstrate understanding of the training on the recommended Receive voucher for to be used. Objectives for Environmental Health & Safety (EH&S): Receive a hazard assessment from each lab Record information about the types of labs and types of hazards present on campus Enable allocation of resources based on hazards present in labs Record training of PIs and Lab Workers on Enable distribution of systemwide-funded to lab workers *For the purposes of this LHAT the term Principal Investigator is used very broadly. It refers to the individual responsible for the oversight of a laboratory and may include individuals with the title of Faculty Principal Investigators, Laboratory Supervisors, Department Laboratory Coordinators, Laboratory Directors, Instrumentation Laboratory Supervisors, and Laboratory Administrators.. UC Laboratory Hazard Assessment v11 UC Regents Page 1 of 28

2 The PI must conduct hazard assessments specific to activities in their laboratories, including when new activities are adopted, or there is a modification of activities. A hazard assessment must occur at least once each calendar year. The Laboratory Hazard Assessment Tool identifies hazards to personnel and specifies personal protective equipment () to be used during work activities. The PI must certify that the LHAT is complete and reflective of activities in their laboratories. EH&S personnel are available to assist with completing the Hazard Assessment form or with reviewing it once it has been completed. Your campus EH&S may also be consulted for specific questions regarding requirements. Principal Investigator: Department: Principal Investigator phone: Principal Investigator Laboratory Safety Contact: Laboratory Safety Contact phone: Laboratory Safety Contact Laboratory name: Laboratory Locations: building(s) / room(s): UC Laboratory Hazard Assessment v11 UC Regents Page 2 of 28

3 Activity Hazard Assessment In this section, the Principal Investigator will: Conduct a hazard assessment of this laboratory to identify activities when is needed to protect the laboratory personnel Certify the hazard assessment for the laboratory The final assessment report will summarize the applicable for the hazards identified in the laboratory. The LHAT is a selection tool only; administrative and engineering controls for specific activities are contained in your laboratory specific or local SOPs. For activities that are described in a laboratory specific Standard Operating Procedure (SOP) or for activities where a Use Authorization (UA) has been issued by a campus safety committee, the specified in that SOP/UA shall take precedence. All Laboratories This laboratory has been designated and posted as free of physical or chemical hazards. Skip all other sections. Active Researcher Adjacent Individuals Attire Attire (direct manipulation) Full length pants or equivalent Closed-toed/closed-heel shoes All personnel in laboratory room: Full length pants or equivalent Closed-toed/closed-heel shoes Under UC Policy, full length pants (or equivalent) and closed-toed/closed-heel shoes must be worn at all times by all individuals who are occupying or entering a laboratory or technical area. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 3 of 28

4 Activity performed Laboratory Hazard Assessment Tool Chemical Hazards I certify that all activities listed in the Chemical Hazards section below are NOT conducted in this laboratory. Yes No Activity in lab Potential Hazards C01. Working with hazardous chemicals (solid, liquid, or gas) C02. Working with hazardous liquids or other materials which create a splash hazard C03. Working with small volumes ( 4L) of corrosive liquids or solids C04. Working with large volumes (>4L) of corrosive liquids or solids C05. Working with small volumes ( 1L) of flammable solvents/materials when no reasonable ignition sources are present C06. Working with large volumes (> 1L) of flammable solvents/materials C07. Working with any quantity of flammable solvents/materials when there are reasonable ignition sources present; or Working in areas where flammable concentrations of vapors or gas may be present Skin or eye damage, potential poisoning through skin contact. Poisoning increased potential for eye and skin damage Eye or skin damage Low probability for a splash hazard Eye or skin damage. Low probability for a splash hazard Skin or eye damage, potential poisoning through skin contact Major Fire. Major skin or eye damage, potential poisoning through skin contact Major Fire. Major skin or eye damage, potential poisoning through skin contact Active Researcher (Direct Manipulation) (chemical splash goggles for large quantities) Chemical splash safety goggles Face shield should be considered Chemical-resistant apron should be considered Chemical-resistant apron Flame-resistant outer gloves should be considered Flame resistant lab coat (NFPA 2112) Flame-resistant outer gloves highly recommended Flame resistant lab coat (NFPA 2112) Adjacent Individuals Flame resistant lab coat (NFPA 2112) UC Laboratory Activity Hazard Assessment 11 UC Regents Page 4 of 28

5 Activity performed Laboratory Hazard Assessment Tool Chemical Hazards I certify that all activities listed in the Chemical Hazards section below are NOT conducted in this laboratory. Yes No Activity in lab Potential Hazards C08. Working with Category 1 or 2 acutely toxic chemicals C09. Working with known or suspect human carcinogens C10. Working with reproductive hazard chemicals (including reproductive toxicants and germ cell mutagens) C11. Working with pyrophoric (air reactive) chemicals or chemicals that in contact with water release flammable gasses (water reactive) Spills, splashes, ingestion, inhalation, absorption Chemicals pose a high level of immediate health risk Spills, splashes, ingestion, inhalation, absorption High hazard cancer-causing agents Spills, splashes, ingestion, inhalation, absorption Agents that affect reproductive capabilities, cause mutation and adversely affect fetal development Severe skin and eye damage Fire Active Researcher (Direct Manipulation) Chemical protective apron for H310 For work outside glove boxes: Face shield should be considered FR rated outer gloves should be considered Flame resistant lab coat (NFPA 2112) Adjacent Individuals All personnel in laboratory room: All personnel in laboratory room: Flame resistant lab coat (NFPA 2112) C12. Working with potentially explosive chemicals. Splash, detonation, flying debris, skin and eye damage, fire Work in inert atmosphere when possible. Face shield should be considered Blast shield should be considered Flame resistant lab coat (NFPA 2112) (or chemical splash goggles) Flame resistant lab coat (NFPA 2112) UC Laboratory Activity Hazard Assessment 11 UC Regents Page 5 of 28

6 Activity performed Laboratory Hazard Assessment Tool Chemical Hazards I certify that all activities listed in the Chemical Hazards section below are NOT conducted in this laboratory. Yes No Activity in lab Potential Hazards C13. Working with Category 2 or higher engineered nanomaterials C14. Minor chemical spill cleanup Inhalation, exposure, dermal exposure Skin or eye damage, respiratory damage Active Researcher (Direct Manipulation) Shoe covers Chemical-resistant apron Adjacent Individuals All personnel in laboratory room: C15. Major chemical spill cleanup Multiple hazards Call for EH&S assistance All personnel must evacuate lab Note: In all cases, chemical splash goggles can be substituted for safety glasses and provide a higher level of protection when working with large quantities of material. For splash or impact protection, either chemical splash goggles or safety glasses need to be worn under face shields. Note: All chemical spills need careful evaluation for the hazards presented and course of action. Minor and Major chemical spills might be determined by the quantities of material spilled or the health hazard presented. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 6 of 28

7 Activity performed Laboratory Hazard Assessment Tool Physical Hazards I certify that all activities listed in the Physical Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard P01. Working with cryogenic liquids P02. Working with very cold equipment, samples, or dry ice P03. Removing sealed vials from liquid nitrogen P04. Working with scalding liquids or hot equipment (e.g., autoclave, water bath, oil bath) P05. Glassware washing P06. Working with loud equipment, noises, sounds, alarms, etc. P07. Working with a high-powered sonicator Major skin, tissue, or eye damage Frostbite, hypothermia Vials may explode upon rapid warming Cuts to face/neck and frostbite to hands Burns resulting in skin or eye damage Lacerations, chemical splash Potential ear damage and hearing loss Ear damage, tissue damage Active Researcher (Direct Manipulation) (chemical splash goggles for large volumes) Face shield should be considered Cryogenic protective gloves Cryogenic protective gloves (possibly warm clothing) Face shield should be considered Cryogenic protective gloves (chemical splash goggles for large volumes) Thermal protective gloves (impermeable insulated gloves for liquids and steam) Hearing protection (consult EH&S for SNR factor needed) Disposable gloves Hearing protection, as necessary (consult EH&S for SNR factor needed) Adjacent Individuals N/A N/A N/A Hearing protection (consult EH&S for SNR factor needed) Hearing protection (consult EH&S for SNR factor needed) UC Laboratory Hazard Assessment v11 UC Regents Page 7 of 28

8 Activity performed Laboratory Hazard Assessment Tool Physical Hazards I certify that all activities listed in the Physical Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard P08. Working with a centrifuge P09. Working with sharps (e.g. needles, razor blades and broken glass) P10. Working with an apparatus containing materials under pressure or vacuum P11. Working with a microtome Imbalanced rotor can lead to broken vials, cuts, exposure, projectiles Cuts, exposure Eye or skin damage Projectiles Cuts, pinch and exposure Active Researcher (Direct Manipulation) Disposable gloves Cut resistant gloves should be considered Face shield should be considered for high risk activities Chemical-resistant apron (for high risk activities) Cut resistant gloves N/A N/A Adjacent Individuals Note: In all cases, chemical splash goggles can be substituted for safety glasses and provide a higher level of protection when working with large quantities of material. For splash or impact protection, either chemical splash goggles or safety glasses need to be worn under face shields. N/A UC Laboratory Activity Hazard Assessment 11 UC Regents Page 8 of 28

9 Activity performed Laboratory Hazard Assessment Tool Biological Hazards I certify that all activities listed in the Biological Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard B01. Working with human or nonhuman primate blood, body fluids, tissues, cells or other potentially infectious material (OPIM) which may contain human bloodborne pathogens (BBP) B02. Working with microbial agents (bacteria, virus, parasites, yeast, fungi, prions), recombinant DNA and/ or biological materials (cells, tissues, fluids) exposed to or likely to contain Risk Group 1 microbial agents or recombinant DNA (BSL-1) B03. Working with microbial agents, recombinant DNA and/or biological materials (cells, tissues, fluids) exposed to or likely to contain Risk Group 2 microbial agents or recombinant DNA (BSL-2) B04. Working microbial agents, recombinant DNA and/or biological materials (cells, tissues, fluids) exposed to or likely to contain Risk Group 2 microbial agents or recombinant DNA for which Biosafety Level 3 practices are required (BSL-2+) Exposure to infectious material, sharps injuries Eye irritation, sharps injury Exposure of infectious material to those who may have personal health issues which make them more susceptible to infection; cross contamination of animal or extra laboratory areas Exposure to infectious material, particularly through broken skin or mucous membranes, sharps injuries Exposure to infectious materials with high risk of exposure by contact with skin or mucous membranes and/ other potential or unknown routs of entry and or increased consequences of exposure Sharps injuries Active Researcher (Direct Manipulation) Eye and mucous membrane protection (as appropriate for operations) Disposable gloves Barrier lab coat impervious to fluids Disposable gloves Additional may be required based on risk assessment by the IBC Double layer of disposable gloves Additional may be required based on risk assessment by the IBC Double layer disposable gloves or Barrier lab coat impervious to fluids Additional may be required based on risk assessment by the IBC Adjacent Individuals All personnel in laboratory room: All personnel in laboratory room: or disposable lab coat UC Laboratory Hazard Assessment v11 UC Regents Page 9 of 28

10 Activity performed Laboratory Hazard Assessment Tool Biological Hazards I certify that all activities listed in the Biological Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard B05. Working with microbial agents, recombinant DNA and/or biological materials (cells, tissues, fluids) exposed to or likely to contain Risk Group 3 microbial agents or recombinant DNA (BSL-3) B06. Working with live animals- only or in conjunction with Risk Group 1 microbial agents or recombinant DNA (ABSL-1) B07. Working infected or potentially infectious live animals alone or in conjunction with Risk Group 2 microbial agents or recombinant DNA (ABSL-2) Exposure to infectious materials with high risk of exposure, particularly through the inhalation route Animal bites, allergies, eye irritation, sharps injury Exposure of infectious material to those who may have personal health issues which make them more susceptible to infection; cross contamination of animal or extra laboratory areas Animal bites, exposure to infectious material, allergies, sharps injury Active Researcher (Direct Manipulation) Double layer disposable gloves Shoe covers or dedicated shoes Disposable closed front lab coat or coveralls (preferred) Respirator (for some work a higher level may be required N95 minimum) Additional may be required based on risk assessment by the IBC Disposable gloves Additional (e.g. puncture resistant gloves) may be required based on risk assessment by the IBC & IACUC. Additional gowning (shoe covers, face mask) may be required for animal welfare purposes. Disposable gloves Bouffant cap Additional (e.g. puncture resistant gloves) may be required based on risk assessment by the IBC & IACUC. Additional gowning (shoe covers, face mask) may be required for animal welfare purposes. Adjacent Individuals All personnel in laboratory room: Double layer disposable gloves Disposable shoe covers or dedicated shoes Closed front Barrier lab coat impervious to fluids or coveralls (preferred) Respirator (for some work a higher level may be required N95 minimum,) All personnel in laboratory room: All personnel in laboratory room: Bouffant cap UC Laboratory Activity Hazard Assessment 11 UC Regents Page 10 of 28

11 Radiological Hazards I certify that all activities listed in the Radiological Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard R01. Working with unsealed radioactive materials including generally licensed radioactive material or devices (e.g., uranyl acetate, thorium nitrate, 32 P-labeled biomolecules) R02. Working with unsealed radioactive materials in hazardous chemicals (corrosives, flammables, liquids, powders, etc.) R03. Working with sealed radioactive sources or devices containing sources of radioactive materials (e.g., liquid scintillation counters, gas chromatographs/electron capture detectors, static eliminators, etc.) Cell damage, potential spread of radioactive materials Cell damage or spread of contamination plus hazards for the specific chemical If sealed source is compromised due to removal from equipment or physical abuse: cell damage, potential spread of radioactive materials Active Researcher (Direct Manipulation) Impermeable gloves or chemical-resistant gloves (chemical splash goggles for splash hazard) Note: Select gloves for applicable chemical hazards above. is not necessary under normal operating instructions Note: Source may not be removed form device except by EH&S or manufacturer. Adjacent Individuals Note: In all cases, chemical splash goggles can be substituted for safety glasses and provide a higher level of protection when working with large quantities of material. For splash or impact protection, either chemical splash goggles or safety glasses need to be worn under face shields. N/A UC Laboratory Hazard Assessment v11 UC Regents Page 11 of 28

12 Activity performed Laboratory Hazard Assessment Tool Laser Hazards I certify that all activities listed in the Laser Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard L01. Open Beam- Performing alignment, trouble-shooting or maintenance that requires working with an open beam and/or defeating the interlock (s) on any Class 3 or Class 4 laser system L02. Open Beam- Viewing a Class 3R laser beam with magnifying optics L03. Open Beam- Working with a Class 3B laser open beam system with the potential for producing direct or specular reflections L04. Open Beam- Working with a Class 4 laser open beam system with the potential for producing direct, specular or diffuse reflections L05. Non-Beam - Handling dye laser materials, such as dyes, chemicals, and solvents L06. Non-Beam- Maintaining and repairing power sources for large Class 3B and Class 4 lasers L07. Enclosed Beam- Using a Class 1 device housing a Class 3B or Class 4 enclosed or embedded laser with the potential for beam exposure during a servicing event. Eye damage Eye damage Eye damage Eye damage, skin damage Cancer, explosion, fire Electrocution, explosion fire Eye damage, skin damage Active Researcher (Direct Manipulation) Optical density and wavelength specific safety glasses based on individual beam parameters Optical density and wavelength specific safety glasses based on individual beam parameters Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes Gloves, safety glasses, NFPA 2112 flame-resistant lab coat or coveralls Electrical isolation mat Electrical protection lab coat (NPFA 70E) or coveralls Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes Adjacent Individuals All personnel in laser use room: Optical density and wavelength specific safety glasses based on individual beam parameters UC Laboratory Hazard Assessment v11 UC Regents Page 12 of 28 N/A All personnel in laser use room: Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes All personnel in laser use room: Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes N/A All personnel in laser use room: Optical density and wavelength specific safety glasses based on individual beam parameters or appropriate clothes

13 Activity performed Non-Ionizing Radiation Hazards I certify that all activities listed in the Non-Ionizing Radiation Hazards section below are NOT conducted in this laboratory Yes No Activity in lab Potential Hazard N01. Working with unshielded sources of ultraviolet radiation. N02. Working with intense infrared emitting equipment (e.g. glass blowing). Conjunctivitis, corneal damage, skin redness Cataracts, burns to cornea Active Researcher (Direct Manipulation) UV face-shield Gloves Appropriate shaded glasses Adjacent Individuals In adjacent area with direct line of sight: UV face-shield In adjacent area with direct line of sight: Appropriate shaded glasses UC Laboratory Activity Hazard Assessment 11 UC Regents Page 13 of 28

14 Unique or Lab-Specific Activities If your lab conducts any additional or unique activities that are not listed above, identify the potential hazards and appropriate then add these activities to the table below. If a lab activity is similar to but somewhat different than one of the common activities listed, include it in this section as well. Activity in lab Potential Hazard Active Researcher (Direct Manipulation) Adjacent Individuals UC Laboratory Hazard Assessment v11 UC Regents Page 14 of 28

15 Certification of Hazard Assessment: Pursuant to 8 CCR 3380(f), I certify that I have performed a hazard assessment for, on date. Accept button to read Certify on behalf of The Regents of the University of California. Name and title of person conducting assessment Name: Date assessment completed: Title: UC Laboratory Hazard Assessment v11 UC Regents Page 15 of 28

16 The following laboratory personnel have reviewed the Laboratory Hazard Assessment Tool specific to this laboratory and have received the following training: Maintain a copy of the signed hazard assessment with lab safety records. 1. What hazards are present in this laboratory 2. When is necessary 3. What is recommended 4. How to properly don, doff, adjust and wear 5. Limitations of 6. Proper care, maintenance, useful life, and disposal of 7. General safety practices (e.g. not wearing outside the laboratory) Lab Name: Name Training Date UC Laboratory Hazard Assessment v11 UC Regents Page 16 of 28

17 Definitions Animal BioSafety Level (ABSL) Animal Biosafety Level 1 (ABSL-1)is assigned for animal work that does not involve biological agents or involves well-characterized agents that are not known to cause disease in immunocompetent humans, and that are of minimal potential hazard to laboratory personnel and the environment. Animal Biosafety Level 2 (ABSL-2) is assigned for animal work with those agents associated with human disease that pose moderate hazards to personnel and the environment. ABSL-2 builds on the practices, procedures, containment equipment, and facility requirements of ABSL-1. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 17 of 28

18 Acutely toxic chemicals may produce a debilitating condition or death after a single exposure or after multiple exposures within a short period of time. They are labeled with a skull and cross bones. An individual may be exposed via any of the routes of exposure shown. Acutely toxic materials are grouped into four categories based on their degree of toxicity by each of these routes of exposure. Category 1, extremely toxic materials, and Category 2, highly toxic materials, present the greatest risk of poisoning. Acute toxins are classified in the US by measured toxicity: Category 1 (oral LD 50 < 5 mg/kg; e.g. aflatoxin, ricin), Category 2 (oral LD50 between 5 and 50 mg/kg; e.g. sodium cyanide), through Category 4 (oral LD 50 between 300 and 2,000 mg/kg). The oral LD 50 for sodium chloride is 5000 mg/kg. Includes GHS codes: H300: Acute toxicity, oral - Fatal if swallowed, Category 1 and 2 H310: Acute toxicity, dermal - Fatal in contact with skin, Category 1 and 2 H330: Acute toxicity, inhalation - Fatal if inhaled, Category 1 and 2 acutely toxic chemicals UC Laboratory Activity Hazard Assessment 11 UC Regents Page 18 of 28

19 adjacent area centrifuges contact with water release flammable gasses corrosive liquids or solids cryogenic liquids The distance (radius) for the adjacent area depends on the material hazards, the lab activity, and the lab configuration. Each laboratory can set distances according to their unique situation, but some examples of suggested distances are: For pipetting small volumes (10 microliters) of acute toxins, the hazardous zone could be 1 meter. For pouring small volumes (1 liter) of acidic solutions, the splash zone could be 2 meters. For working with modest volumes (4 liters) of flammable liquids, the flash fire zone could be 3 meters. For working with materials under pressure, the hazardous zone could be 10 meters. For working with explosives, the danger zone is the entire laboratory. Centrifuges separate materials based on density, and they employ a rotor that spins the sample at a high rate of rotation. Centrifuge rotors that are damaged or unbalanced have resulted in injury, even death, because of the energy momentum released due to failure. Includes GHS codes: H260: In contact with water releases flammable gases which may ignite spontaneously H261: In contact with water releases flammable gas Examples include alkyllithium reagents, calcium carbide, Grignard reagent solutions Corrosives are a classification of chemicals that have both physical and health hazards. A chemical which is corrosive to metals will materially damage, or even destroy, metals by chemical action. A skin corrosive will cause irreversible damage to the skin; namely, visible necrosis through the epidermis and into the dermis, following contact with the substance. Most strong acids (aqueous solutions with a ph less than 2) or strong bases (aqueous solutions with a ph greater than 12.5) are corrosive. It is equally important to know that there are numerous corrosive materials that have a neutral ph, examples include phenol and triethylamine. Cryogens are substances cooled to very low temperatures. Liquid nitrogen is an example. These substances are considered hazardous because of the potential for cryogenic burns, asphyxiation, and catastrophic container failure. Due to the large coefficient of thermal expansion for cryogens, the large volume of gas evolved can quickly displace atmospheric oxygen. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 19 of 28

20 Engineered Nanomaterials (ENMs): An Engineered Nanomaterial is any intentionally produced material with any external dimension in the nanoscale. It is noted that neither 1 nm nor 100 nm is a bright line and some materials are considered engineered nanomaterials that fall outside this range. For example, Buckyballs are also included even though they have a size <1 nm. Excluded are materials that are on the nanoscale, but do not have properties that differ from their bulk counterpart and include micelles and single polymers. engineered nanomaterials For more information see: the UC Nanotoolkit - Working Safely with Engineered Nanomaterials in Academic Research Settings UC Laboratory Activity Hazard Assessment 11 UC Regents Page 20 of 28

21 Flammable chemicals are materials that can ignite easily. Flammables may be either gas, aerosol, liquid, or solid. Flammable liquid is defined under the new GHS standards as a liquid having a flash point of not more than 93 C (200 F). (Note that the older US limit was 38 C (100 F).) The flash point of a volatile material is the lowest temperature at which the vapors form an ignitable mixture in air. Includes GHS codes: H220: Flammable gases- Extremely flammable gas, Category 1 H221: Flammable gases - Flammable gas, Category 2 H222: Flammable aerosols - Extremely flammable aerosol, Category 1 H223: Flammable aerosols - Flammable aerosol, Category 2 H224: Flammable liquids - Extremely flammable liquid and vapor, Category 1 H225: Flammable liquids - Highly Flammable liquid and vapor, Category 2 H226: Flammable liquids - Flammable liquid and vapor, Category3 H227: Flammable liquids - Combustible liquid, Category 4 H228: Flammable solids - Flammable solid, Category 1 and 2 Examples: flammable solvents/materials UC Laboratory Activity Hazard Assessment 11 UC Regents Page 21 of 28

22 Full length pants or equivalent Flash Point Risk Group 1 microbial agents Risk Group 2 microbial agents Risk Group 3 microbial agents hazardous chemicals hazardous liquids Full length pants or equivalent should cover all the skin down to one s shoes to protect skin from spills. Loose fitting clothes are best to avoid spilled material being held against skin. In all instances, cotton clothing is preferred over synthetic material that is less chemical and flame resistant. The flash point of a volatile material is the lowest temperature at which the vapors form an ignitable mixture in air. Biological Agents are classified into four Risk Groups (RGs) according to their relative pathogenicity for healthy adult humans by the following criteria: Risk Group 1 (RG1) agents are not associated with disease in healthy adult humans. Example: Bacillus subtillis. Risk Group 2 (RG2) agents are associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available. Examples: Escherichia coli, Helicobacter pylori, Staphylococcus aureus, Adenoviruses, Rhinoviruses. Risk Group 3 (RG3) agents are associated with serious or lethal human disease for which preventive or therapeutic interventions may be available. Examples: Human immunodeficiency virus, Yellow fever virus. Risk Group 4 (RG4) agents are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are not usually available. Example: Lassa virus. The University of California does not possess or use RG4 agents. For a searchable database of infectious agents, see: The hazardous properties of chemicals can be broken into two broad divisions physical hazards and health hazards. Chemicals with physical hazards could include reactives, flammables, oxidizers and chemicals that are corrosive to metals. Chemicals with health hazards could include skin corrosives, sensitizers, toxic substances, and carcinogens. Includes any GHS H code that begins with : H2## Physical Hazards H3## Health Hazards UC Laboratory Activity Hazard Assessment 11 UC Regents Page 22 of 28

23 Carcinogens are chemical substances which induce cancer or increase its incidence. Carcinogens produce delayed health effects that may take years to manifest. Many carcinogens cause malignant tumors in humans and animals. A chemical is considered a select carcinogen if it has been evaluated and listed as a carcinogen by - The International Agency for Research n Cancer (IARC) - The National Toxicology Program (NTP) - OSHA Includes GHS codes: H350: Carcinogenicity - May cause cancer, Category 1A, 1B H351: Carcinogenicity - Suspected of causing cancer, Category 2 Examples: Benzene Dichloromethane (methylene chloride) Formaldehyde Lead Asbestos human carcinogens UC Laboratory Activity Hazard Assessment 11 UC Regents Page 23 of 28

24 ignition sources microtome An open flame or spark or even hot surfaces. A microtome is a tool used to cut extremely thin sections, or slices, of a material. The very sharp blades necessary for a microtome to section tissue can cause serious cuts during cleaning or sectioning. Mechanized microtomes can cause pinch injuries. Explosive chemicals can release tremendous amounts of destructive energy rapidly. If not handled properly, these chemicals can pose a serious threat to the health and safety of laboratory personnel, building occupants, and chemical waste handlers. Potentially explosive chemicals (PECs) are chemicals (or combinations thereof) that may cause a sudden release of energy and heat when subjected to sudden shock, pressure, or high temperature. Most chemicals that are used in research and teaching laboratories are stable and non-explosive at the time of purchase. Over time, some chemicals can oxidize, become contaminated, dry out, or otherwise destabilize to become PECs (e.g., isopropyl ether, and picric acid). potentially explosive chemicals May include GHS codes: H201: Explosives - Explosive; mass explosion hazard, Division 1.1 H202: Explosives - Explosive; severe projection hazard, Division 1.2 H203: Explosives - Explosive; fire, blast or projection hazard, Division 1.1 H240: Self-reactive substances and mixtures; and Organic peroxides - Heating may cause an explosion, Type A H241: Self-reactive substances and mixtures; and Organic peroxides - Heating may cause a fire or explosion, Type B H271: Oxidizing liquids; or Oxidizing solids - May cause fire or explosion; strong oxidizer, Category 1 Pyrophorics are liable to ignite upon contact with air. pyrophoric Includes GHS codes: H250: Pyrophoric liquids; or Pyrorophoric solids - Catches fire spontaneously if exposed to air, Category 1 H251: Self-heating substances and mixtures - Self-heating; may catch fire, Category 1 H252: Self-heating substances and mixtures - Self-heating in large quantities; may catch fire, Category 2 Examples include tert-butyllithium solutions, potassium metal, triethylborane, white phosphorous UC Laboratory Activity Hazard Assessment 11 UC Regents Page 24 of 28

25 Reproductive Hazard chemicals are either classified as Germ Cell Mutagens or Toxic to Reproduction. These materials can affect reproductive health in both men and women. Adverse effects include impotence, low spec count, infertility, miscarriage and birth defects. Includes GHS codes: H340: Germ cell mutagenicity - May cause genetic defects, Category 1A, 1B H341: Germ cell mutagenicity - Suspected of causing genetic defects, Category 2 H360: Reproductive toxicity - May damage fertility or the unborn child, Category 1A, 1B H361: Reproductive toxicity - Suspected of damaging fertility or the unborn child, Category 2 H362 Reproductive toxicity, effects on or via lactation - May cause harm to breast-fed children Examples: carbon disulfide, many anticancer agents, lead, thalidomide For a list, see: reproductive hazards. open beam In an open beam laser the entire beam path is not totally enclosed. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 25 of 28

26 Laser Hazard Class Laboratory Hazard Assessment Tool Lasers have been classified by wavelength and maximum output power into four classes and a few subclasses. These are typically identified with a warning label on the laser device indicating the hazard class. Laser pointers are in Class 3R. Laser Hazard Class Class 1 Class 2 Class 3R Class 3B Class 4 A Class 1 laser is safe under all conditions of normal use. A Class 2 laser is safe because the blink reflex will limit the exposure to no more than 0.25 seconds. It only applies to visible-light lasers ( nm). A Class 3R laser is considered safe if handled carefully, with restricted beam viewing. A Class 3B laser is hazardous if the eye is exposed directly, but diffuse reflections such as those from paper or other matte surfaces are not harmful Class 4 is the highest and most dangerous class of laser. By definition, a class 4 laser can burn the skin, or cause devastating and permanent eye damage as a result of direct, diffuse or indirect beam viewing. These lasers may ignite combustible materials, and thus may represent a fire risk. Each laboratory space where biohazardous materials are used is assigned one of three internationally recognized biosafety levels (BSL). The biosafety level is commensurate with the degree of risk posed by the biohazardous materials and the activities carried out with those materials. Biosafety Level UC Laboratory Activity Hazard Assessment 11 UC Regents Page 26 of 28

27 other potentially infectious material (OPIM) Servicing event Other Potentially Infectious Materials (OPIM) include semen, vaginal secretions and pericardial fluid which entail potential risk for Bloodborne Pathogen exposure. According to the CDC, over 200 different diseases can result from exposure to blood. The most common Bloodborne pathogens are HIV, hepatitis B virus (HBV) and hepatitis C virus (HCV) It should be noted that during periods of service or maintenance, controls appropriate to the class of the embedded laser may be compromised (perhaps on a temporary basis) when the beam enclosures are removed and beam access is possible. Beam access during maintenance or service procedures will not alter the Class I status of the laser during operation. UC Laboratory Activity Hazard Assessment 11 UC Regents Page 27 of 28

28 Information Page 1) This Laboratory Hazard Assessment Tool is modeled after one developed at UCLA. It was created by the Information Technology Services group working at UC Davis. 2) Chemical hazards are classified according to the Globally Harmonized System of Classification and Labeling of Chemicals (GHS). 3) This link provides a listing of GHS Hazard Codes. 4) This Hazard Assessment Tool focuses on for specific hazards. Principal investigators and lab personnel also must use Engineering Controls, Administrative Controls, and safe work practices to minimize risks posed by hazardous materials. 5) For information on working safely with engineered nanomaterials, see: OR UC Laboratory Activity Hazard Assessment 11 UC Regents Page 28 of 28