PROGRAMME AND ABSTRACT BOOK

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1 4 th INTERNATIONAL PARTICIPATED ENTOMOPATHOGENS AND MICROBIAL CONTROL SYMPOSIUM September 2013 ARTVIN, TURKEY PROGRAMME AND ABSTRACT BOOK Responsibilities of all abstracts contained in this book belong to the authors.

2 4 th INTERNATIONAL PARTICIPATED ENTOMOPATHOGENS AND MICROBIAL CONTROL SYMPOSIUM September 2013 ARTVIN, TURKEY Cover Design Ġbrahim Burak KALEOĞLU Composition Research Assist. Halil Ġbrahim GÜLER Print Demokrat Coruh Printing September

3 HONORARY CHAIRMEN Kemal CĠRĠT (Governer of Artvin) Prof. Dr. Mehmet DUMAN (Rector of Artvin Çoruh University) Prof. Dr. Yılmaz ALTUN (Dean of the Faculty of Arts and Sciences) THE HEAD OF THE ORGANIZING COMMITTEE Assist. Prof. Dr. Nurcan ALBAYRAK ĠSKENDER ORGANIZING COMMITTEE Assist. Prof. Dr. Gökhan BAYRAMOĞLU Assist. Prof. Dr. AyĢegül BAYRAMOĞLU Assist. Prof. Dr. Melahat ÖZCAN Assist. Prof. Dr. Handan KARAOĞLU Assist. Prof. Dr. Suat ÇOLAK Research Assist. Mehmet DEMĠRALAY Research Assist. Halil Ġbrahim GÜLER Research Assist. AyĢegül SARAL Research Assist. Mustafa Çağatay KORKMAZ 3

4 SCIENTIFIC COMMITTEE Zihni DEMĠRBAĞ, Karadeniz Technical University, Turkey Ömer Faruk ALGUR, Atatürk University, Turkey Fikrettin ġahġn, Yeditepe University, Turkey Selçuk HAZIR, Adnan Menderes University, Turkey Basil M. ARIF, Canadian Forest Research Center, Canada Edwin E. LEVIS, University of California, USA Tariq BUTT, University of Wales Swansea, United Kingdom Johannes JEHLE, Julius Kuehn Institute, Germany Sebahattin ÖZCAN, Ankara University, Turkey Jorgen EILENBERG, University of Copenhagen, Denmark Sabahat SULLIVAN, Ondokuz Mayıs University, Turkey Mustafa AVCI, Süleyman Demirel University, Turkey ġaban TEKĠN, GaziosmanpaĢa University, Turkey Ġsmail Alper SUSURLUK, Uludağ University, Turkey Madoka NAKAI, Tokyo University of Agriculture and Technology Fuchu, Japan Ramazan CANHĠLAL, Erciyes University, Turkey Nurcan ALBAYRAK ĠSKENDER, Artvin Çoruh University, Turkey Meriç KUMBAġLI, Ġstanbul University, Turkey Mohammadreza Rezapanah, Iranian Research Institute of Plant Protection, Iran Temel GÖKTÜRK, Artvin Çoruh University, Turkey Madea BURJANADZE, Vasil Gulisashvili Forest Institute, Georgia Itamar GLAZER, The Volcani Center, Israel Ali SEVĠM, Ahi Evran University, Turkey Engin KILIÇ, Erzincan University, Turkey Serkan ÖRTÜCÜ, Erzurum Technical University, Turkey Umut TOPRAK, Ankara University, Turkey 4

5 INVITED SPEAKERS Prof. Dr. Zihni DEMĠRBAĞ, Karadeniz Techinal University, Turkey Prof. Dr. Ömer Faruk ALGUR, Atatürk University, Turkey Prof. Dr. Johannes JEHLE, Institute of Julius Kuehn, Germany Prof. Dr. Mohammadreza Rezapanah, Iranian Research Institute of Plant Protection, Iran Prof. Dr. Fikrettin ġahġn, Yeditepe University, Turkey Prof. Dr. Juan FERRE, University of Valencia, Spain Prof. Dr. Selçuk HAZIR, Adnan Menderes University, Turkey Prof. Dr. Edwin E. LEVIS, California University, USA Prof. Dr. Basil M. ARIF, Great Lakes Forestry Centre, Canadian Forest Service, Canada Prof. Dr. Itamar GLAZER, Volcani Centre, Israel Prof. Dr. Tariq BUTT, Swansea University, England Prof. Dr. Jorgen EILENBERG, University of Copenhagen, Denmark Prof. Dr. Zhihong HU, Wuhan Institute of Virology, Chinese Academy of Sciences, China Prof. Dr. Madea BURJANADZE, Vasil Guliashvili Forest Institute, Georgia Prof. Dr. ġaban TEKĠN, GaziosmanpaĢa University, Turkey Assoc. Prof. Dr. Temel GÖKTÜRK, Artvin Coruh University, Turkey Assoc. Prof. Dr. Ġsmail DEMĠR, Karadeniz Techinal University, Turkey Assoc. Prof. Dr. Madoka NAKAI, Tokyo University of Agriculture and Technology, Fuchu, Japan Assist. Prof. Dr. Serkan ÖRTÜCÜ, Erzurum Techinal University, Turkey Dr. Umut TOPRAK, Ankara University, Turkey 5

6 Dear Colleagues, We are excited to host the 4 th International Participated Entomopathogens and Microbial Control Symposium in Land Adjacent to the Sky, Artvin, shaped by Çoruh River flowing wildly through steep mountains. On behalf of Faculty of Science and Letters Biology Department of Artvin Çoruh University developing rapidly since its establishment in Turkey s green heaven Artvin, it s our pleasure to invite you to the 4 th International Participated Entomopathogens and Microbial Control Symposium to be held from September 11 th to 14 th, This symposium will be a great opportunity for scientists and researchers to come together, to share the most up-to-date scientific research and advances in the fields of entomology and microbial control, gaining importance day by day and to exchange opinions on recent developments. The symposium will be held in two languages, English and Turkish. Looking forward to meeting you in the 4 th International Participated Entomopathogens and Microbial Control Symposium, where you can enjoy both the rich scientific content and viridity of nature On behalf of the Organizing Committee Assist. Prof. Dr. Nurcan ALBAYRAK ĠSKENDER 6

7 REGISTRY PROGRAMME 11 SEPTEMBER 2013 WEDNESDAY MAIN SALOON 10:00 REGISTRATION 13:30-15:00 PIANO RECITAL AND OPENING CEREMONY SESSION 1 Session Chair Session Assistant (MAIN SALOON) Prof. Dr. Basil M. ARIF Assoc. Prof. Dr. Remziye NALÇACIOĞLU 15:00-15:30 Zihni DEMĠRBAĞ 15:30-16:00 CATERING SESSION 2 Session Chair Session Assistant (BLUE SALOON) Prof. Dr. Selçuk HAZIR Assoc. Prof. Dr. İsmail DEMİR 16:00-16:30 Johannes JEHLE 16:30-17:00 Basil M. ARIF 18:30 WELCOME COCTAIL 7

8 12 SEPTEMBER 2013 THURSDAY SESSION 3 Session Chair Session Assistant RED SALOON Prof. Dr. Edwin E. LEWIS Assoc. Prof. Dr. Kazım SEZEN 09:00-09:30 Juan FERRE 09:30-10:00 Ömer Faruk ALGUR 10:00-10:30 Fikrettin ġahġn 10:30-10:45 CATERING SESSION 4 Session Chair Session Assistant RED SALOON Prof. Dr. Ramazan CANHİLAL Assoc. Prof. Dr. İlker KEPENEKÇİ 10:45-11:15 Selçuk HAZIR 11:15-11:45 Edwin E. LEWIS 11:45-12:15 Itamar GLAZER 12:15-13:15 LAUNCH RED SALOON BLUE SALOON SESSION 5 Session Chair Prof. Dr. Fikrettin ŞAHİN Session Assistant Assist. Prof. Dr. Nurcan ALBAYRAK İSKENDER Session Chair Prof. Dr. Şaban TEKİN Session Assistant Assoc. Prof. Dr. Temel GÖKTÜRK 13:30-13:45 Manana KERESELIDZE Kuralay MAZARZHANOVA 13:45-14:00 Ahmet KATI Gürsel KARACA 14:00-14:15 Ardahan ESKĠ Ġlker KEPENEKÇĠ 14:15-15:00 CATERING 15:30 BARBECUE and KAFKASÖR EXCURSION 8

9 SESSION 9 13 SEPTEMBER 2013 FRIDAY SESSION 6 Session Chair Session Assistant RED SALOON Prof. Dr. Zihni DEMİRBAĞ Assist. Prof. Dr. Hacer MURATOĞLU 09:00-09:30 Zhihong HU 09:30-10:00 Madoka NAKAI 10:00-10:30 Mohammedreza REZAPANAH 10:30-11:00 CATERING SESSION 7 Session Chair Session Assistant RED SALOON Prof. Dr. M. REZAPANAH Assist. Prof. Dr. Serkan ÖRTÜCÜ 11:00-11:30 Umut TOPRAK 11:30-12:00 ġaban TEKĠN 12:00-12:30 Medea BURJANADZE 12:30-13:30 LAUNCH SESSION 8 Session Chair Session Assistant RED SALOON Prof. Dr. Juan FERRE Assist. Prof. Dr. Engin KILIÇ 13:30-14:00 Ġsmail DEMĠR 14:00-14:30 Jorgen EILENBERG 14:30-15:00 Tariq BUTT 15:00-15:15 A. Özgür ATEġ 15:15-15:45 CATERING 15:45-18:30 POSTER PRESENTATIONS 19:00 GALA DINNER 9

10 SESSION SEPTEMBER 2013 SATURDAY SESSION 10 Session Chair Session Assistant RED SALOON Prof. Dr. Medea BURJANADZE Assoc. Prof. Dr. Hatice KATI 09:15-09:30 Nurcan ALBAYRAK ĠSKENDER 09:30-10:00 Temel GÖKTÜRK 10:00-10:30 Serkan ÖRTÜCÜ 10:30-11:00 CATERING 11:00-12:00 CLOSING 12:00-12:45 LAUNCH 13:00 CAMILI EXCURSION 10

11 TABLE OF CONTENTS Oral Presentations: Functional analysis of AMEV serine/threonin protein kinase gene and its role in host specificity Hacer MURATOĞLU, Duygu BEKĠRCAN, Remziye NALÇACIOĞLU, Srini PERERA, Basil M. ARIF, Zihni DEMĠRBAĞ...18 Use of Insect Microflora in Biotechnological Studies Ömer Faruk ALGUR Biological Control of Pest Insects in Germany - Presence and Future Johannes A. JEHLE...20 Codling moth control by Cydia pomonella granulovirus in organic apple orchards: lessons from the differences Mohammadreza REZAPANAH...21 Biological effects of novel commercial formulations developed for mosquito control Fikrettin ġahġn, M. Muge YAZICI, Huseyin ÇETĠN, Yusuf KAYA 22 New insights into the mode of action of Bacillus thuringiensis Vip3A insecticidal proteins Juan FERRÉ, Maissa CHAKROUN, Yolanda BEL, Núria BANYULS, Baltasar ESCRICHE...23 Entomopathogenic nematodes: evaluation of different application methods and their persistence in the soil in the presence of a natural enemy Selçuk HAZIR, Karthik R. RAMALINGAM...24 Ecological and behavioral theory lay the groundwork for successful biological control with entomopathogenic nematodes Edwin E. LEWIS...25 Use of Entomopathogenic Nematodes Against Key Pests in Israel Itamar GLAZER26 Enhancing the efficacy of Entomopathogenic Fungi Tariq M. BUTT..27 The three major taxonomic groups of insect pathogenic fungi: phylogeny, life cycle with insects and coevolution Jørgen EILENBERG.28 Comparative proteomics of HearNPV budded and occlusion-derived virus Zhihong HU...29 Pathogens and nematodes of bark beetles (Coleoptera: Scolytidae) in Georgia coniferous forest Medea BURJANADZE.30 Use of Entomopathogens in The Biological Control of Ticks ġaban TEKĠN, Adem KESKIN.31 Viral biocontrol agents, past, present and future: A Canadian perspective Basil M. ARIF...32 The effects of Bacillus thuringiensis and Neemazal on larvaes of Hypantria cunea (Drury) (Lepidoptera: Arctiidae) damaging on leafed trees Temel GÖKTÜRK, Ömer Naci KAYA,YaĢar AKSU

12 Entomopathogenic fungi: A promising biological control agent against bark beetles Ġsmail DEMĠR, Seda KOCAÇEVĠK, Ali SEVĠM, Mahmut EROĞLU, Zihni DEMĠRBAĞ...34 Potential of slow-killing insect viruses to control leaf-rollers in tea fields Madoka NAKAI A Study on the Production, Purification and Acaricidal Activity of a Red Pigment from Beauveria bassiana isolate Paf04 Serkan ORTUCU, Murat OZDAL, Nurcan ALBAYRAK ISKENDER, Omer Faruk ALGUR...36 The First Study on Pathogenicity of Entomopathogenic Fungi Beauveria bassiana to Control The Little Spruce Sawfly Pristiphora abietina (Christ.) (Hymenoptera: Tenthredinidae) in Turkey Nurcan ALBAYRAK ISKENDER, Serkan ORTUCU, AyĢegül SARAL, YaĢar AKSU.37 Investigations on bacteria as a potential biological control agents on corn stalk borer, Sesamia nonagrioides (Lef.) (Lepidoptera: Noctuidae) Ardahan ESKĠ, Mustafa GÜLLÜ, Filiz ÖZKAN ÇAKICI, Zihni DEMĠRBAĞ, Ġsmail DEMĠR...38 First results on the susceptibility of two forest pest insects for a Georgian isolate of Isaria fumosorosea Manana KERESELIDZE, Andreas LINDE, Daniela PILARSKA, Slavimira DRAGANOVA...39 Development of methods for the selection of strains of entomopathogenic fungus Beauveria bassiana (Bals.) Vuill., effective against of bark beetle (Ips sexdentatus Boern., Ips hauseri Reitter) Kuralay MAZARZHANOVA, Anastasiya LUKINA, Nurzhan MUKHAMADIYEV, Nursagım AġIKBAEV...40 Baculuvirus-insect peritrophic matrix interaction: Can we develop more effective baculoviruses using the knowledge on enhancin-insect intestinal mucins substrate specificity? Umut TOPRAK, Dwayne D. HEGEDUS, Martin A. ERLANDSON, David THEILMANN..41 Effects of Some Entomopathogen Fungi Against Tuta absoluta (Meyrick) (Lepidoptera: Gelehiidae) Gürsel KARACA, Burcu AÇILOĞLU, Hatice ACARBULUT ve Ġsmail KARACA..42 Determination Of Bioactive Entomopathogen Bacteria Scale-Up Strategies Ahmet KATI, Ali Özhan AYTEKĠN, Ġkbal Agah ĠNCE, Fikrettin ġahġn.43 Evaluation of aqueous suspension and infected cadaver application of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against root-knot nematodes (Tylenchida: Meloidoginidae) Ġlker KEPENEKCI, Emre EVLICE, Selçuk HAZIR and Adnan TÜLEK...44 Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, as biological control agents against the June beetle, Polyphylla fullo (L.) Özgür ATEġ, Burcu AÇILOĞLU and Fedai ERLER...45 Poster Presentations: Development of a New Bio-Insecticide For Controlling The Lepidopteran Pests Kazım SEZEN, Remziye NALÇACIOĞLU, Ġsmail DEMĠR, Hüseyin TEPE, Ġslam YILDIZ, Zihni DEMĠRBAĞ Isolation, characterization and insecticidal activity of Bacillus thuringiensis from warehouses in Trabzon Meriç DEMELĠ, Kazım SEZEN, Zihni DEMĠRBAĞ

13 The effect rates of Bacillus thuringiensis and Neemazal using in Biological control of Lymantria dispar (L.) (Lepidoptera:Lymantriidae) YaĢar AKSU, Cihangir DEDEAĞAOĞLU, Berna ÇELĠK GÖKTÜRK..49 Morphological and Molecular Characterization of Natural Entomopathogenic Fungi Özlem GÜVEN, Fatma Gül ÖZÇELĠK.50 Isolation and characterization of a parasitic nematode, Oscheius myriophila (Nematoda: Rhabditida), associated with European mole cricket, Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae) Zeynep ERBAġ, Zihni DEMIRBAĞ, Ġsmail DEMĠR...51 The isolation, characterization and pathogenicity of bacteria from Ephestia (Cadra) cautella Ġslam YILDIZ, Kazım SEZEN, Zihni DEMĠRBAĞ.52 In vitro characterization of Chilo iridescent virus 012L encoded protein YeĢim AKTÜRK DĠZMAN, Cemal SANDALLI, Zihni DEMĠRBAĞ, Remziye NALÇACIOĞLU..53 Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae): Efficacy against Leptinotarsa decemlineata L. (Coleoptera: Chrysomelidae) and Agelastica alni L. (Coleoptera: Chrysomelidae) Zeynep ERBAġ, Zihni DEMIRBAĞ, Ġsmail DEMĠR Screening of entomopathogenic fungi against the Grey corn weevil, Tanymecus dilaticollis Gyll. (Coleoptera: Curculionidae) Ġsmail DEMĠR, Mustafa GÜLLÜ, Emine SÖNMEZ, Seda KOCAÇEVĠK, Zihni DEMĠRBAĞ.55 Highly Effective Bacterial Agents against Cimbex quadrimaculatus (Hymenoptera: Cimbicidae): Isolation of Bacteria and Their Insecticidal Activities Ġsmail DEMĠR, Filiz ÖZKAN ÇAKICI, Halil BOLU, Ġnanç ÖZGEN, Zeynep ERBAġ, Zihni DEMĠRBAĞ...56 Host related transactivation of serine / threonine protein kinase gene of Amsacta moorei entomopoxvirus Duygu BEKĠRCAN, Hacer MURATOĞLU, Remziye NALÇACIOĞLU, Basil M. ARIF, Zihni DEMĠRBAĞ..57 Pathogenicity of Beauveria pseudobassiana from Dendroctonus micans Kugel. (Coleoptera: Curcunlionidae) against Rhizophagus grandis Gyll. (Coleoptera: Rhizophagidae) Seda KOCAÇEVĠK, Ali SEVĠM, Zihni DEMĠRBAĞ, Mahmut EROĞLU, Ġsmail DEMĠR...58 Amsacta moorei entomopoxvirus encodes a functional protein kinase Hacer MURATOĞLU, Remziye NALÇACIOĞLU, Basil M. ARIF, Zihni DEMĠRBAĞ..59 Determination of entomopathogenic bacteria species in Dermacentor marginatus and Rhipicephalus annulatus (Acari: Ixodidae) Adem KESKIN, Ahmet BURSALI, Hüseyin TEPE, ġaban TEKIN...60 Determination of entomopathogenic bacteria species in Hyalomma marginatum and Hyalomma excavatum (Acari: Ixodidae) Ahmet BURSALI, Adem KESKĠN, Hüseyin TEPE, ġaban TEKĠN 61 The effect of plant quality on survival of Lymantria dispar (Lepidoptera: Lymantriidae) larvae infected by a nucleopolyhedrovirus Sibel ÇAVDAR, Oguzhan YANAR, Elif Fatma TOPKARA, Ġsmail DEMĠR.62 Characterization of Bacillus thuringiensis strains isolated from the soil samples in Giresun Island Türkmen AYAKSIZ, Hatice KATI

14 The effect of boric acid on production of Bacillus thuringiensis insecticidal crystal protein and bioactivity against Cadra cautella larvae Burcu ġahġn, Mehlika ALPER, Bekir ÇÖL, Oktay DURSUN, Hatice GÜNEġ...64 Determination of bacteriom of some hard tick (Acari: Ixodidae) species using molecular methods Haktan ÇAĞLAYAN, ġaban TEKĠN, Ġsa KARAMAN, Ahmet BURSALI, Adem KESKĠN. 65 Susceptibility of the noxious social insects Blatta orientalis and Blattella germanica cockroaches to infection by entomopathogenic nematodes Nona MIKAIA...66 Role of sexuality in the entomopathogenic nematode, Heterorhabditis bacteriophora on heritability of effectiveness to hybrid strains Tufan Can ULU, Yasemin KONGU and Ġsmail Alper SUSURLUK...67 Determination of Antibiotic Resistance of Some Pathogenic Fungi Adem ĠMALI, Zeynep BULġAN, Birgül ĠLHAN, Ferudun KOÇER..68 Sancassania polyphyllae (Acari: Acaridae) is attracted to its phoretic host larva killed by the entomopathogenic nematodes (Steinernematidae) Mehmet KARAGÖZ, Ġbrahim ÇAKMAK, Derya ULUĞ, Selçuk HAZIR..69 Entomopathogenic Fungi Isolated From Wheat Fields in the Villages at Kahramanmaraş City Center Özlem GÜVEN, Cihat KOZ, Ġsmail KARACA 70 Survey On The Control Possibility Of Ephestiakuehniella Using Trichoderma Atroviridae Under Laboratory Conditions D. Zafari, S. Azimiyan, H. Madadi, F. Ghobadi Anvar.71 Taguchi s method in reducing the experiment numbers of production optimizing of Helicoverpa armigera nucleopolyhedrovirus H. ASSEMI, M. REZAPANAH, A. SAJJADI An effective bacterial isolate for the microbial control of Lobesia botrana (Lep.:Tortricidae) Lida MOHAMMAD GHOLIZAD, Zihni DEMĠRBAĞ...73 A new entomopathogenic fungus: Myriodontium keratinophilum (Ascomycota: Myriodontium) from Gryllotalpa gryllotalpa L. (Orthoptera: Gryllotalpidae) Emine SÖNMEZ, Ali SEVĠM, Zihni DEMĠRBAĞ, Ġsmail DEMĠR...74 Genetic diversity of isolates of Helicoverpa armigera nucleopolyhedrovirus on tobacco budworm using PCR-RFLP technique A. SAJJADI, H. ASSEMI, M. REZAPANAH..75 Entomopathogens & Environmental Sustainability Aysel KEKĠLLĠOĞLU..76 The Effects of Entomopathogenic Fungus [Purpureocillium lilacinum TR1 (syn: Paecilomyces lilacinus)] on the Black Cherry Aphid [Myzus cerasi Fabricus (Hemiptera: Aphididae)] Adults Ilker KEPENEKCI, Burcu ĠNAL, Emre EVLICE, Erçin OKSAL...77 Identification of Entomopathogenic fungi, Purpureocillium lilacinum TR1 (syn: Paecilomyces lilacinus) by classical (morphologic and morphometric properties) and molecular methods Ġlker KEPENEKÇI, Emre EVLĠCE, Erçin OKSAL..78 Determination of efficacy of entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) against a stored crop pest, Sitophilus oryzae (Coleoptera: Curculionidae) Ramazan CANHĠLAL, Fatmanur BORAZAN, Sevim DOĞAN, Yunus Emre ÖZDEMIR, Gizem EġGĠN, Hüseyin AKSOY

15 Investigation of bioactivities of natural Bacillus thuringiensis isolates on Ephestia kuehniella and Plodia interpunctella Mehlika ALPER, Hilal TUNCA, Hatice GÜNEġ.80 Transgenic Bacillus thuringiensis crops Mehlika ALPER81 The studies to increase the insecticidal effect of Cry toxins Mehlika ALPER82 Determination of the presence of entomopathogenic fungi in the region of Kayseri province and territory M. Alper ALTINOK, Adeviye AKPINAR, H. Handan ALTINOK.83 Ecological Diversity Of Entomopathogens Aysel KEKĠLLĠOĞLU, Merve KÖKSAL 84 Future Benefıts Of Insects Pathogens Aysel KEKĠLLĠOĞLU..85 Protein-Protein Interactions Of The Structural Proteins Of Chilo Iridescent Virus Emine ÖZġAHĠN, Monique van OERS, Remziye NALÇACIOĞLU, Just VLAK, Zihni DEMĠRBAĞ 86 Virulance of some entomopathogen bacteria against Chrysomelidae, Colorado potato beetle (Leptinotarsa decemlineata L.) and Alder leaf beetle (Agelastica alni L.) Ardahan ESKĠ, Zihni DEMĠRBAĞ, Ġsmail DEMĠR.87 Metarhizium anisopliae: A promising fungal agent against Gryllotalpa gryllotalpa L. (Orthoptera: Gryllotalpidae) Emine SÖNMEZ, Ali SEVĠM, Zihni DEMĠRBAĞ, Ġsmail DEMĠR 88 Elaboration of integrated plant protection from the fall webworm in urban environments Tsisa CHKHUBIANISHVILI, Iatamze MALANIA, Manana KAKHADZE...89 Role of Prodigiosin on the Acaricidal Activity of Entomopathogen Serratia marcescens Serkan ÖRTÜCÜ, Murat ÖZDAL, Nurcan ALBAYRAK ĠSKENDER, Mehmet Nuri AYDOĞAN.. 90 Functional analysis of Chilo Iridescent Virus zinc-binding matrix metalloproteinase genes Aydın YEġĠLYURT, Hacer MURATOĞLU, Zihni DEMĠRBAĞ, Remziye NALÇACIOĞLU. 91 Efficacy of Entomopathogenic Nematodes Against (Operopthera brumata & Erannis defoliaria) (Lepidoptera: Geometridae) in Field Conditions Manana LORTKIPANIDZE, Oleg GORGADZE, Medea BURJANADZE, Madona KUCHAVA, Mzia KOKHIA, Nana GRATIASHVILIA, Nino GABROSHVILI Study of Entomopathogenic Nematodes Susceptibility to Coccidaes Mariam CHUBINISHVILI, Rusudan SKHIRTLADZE, Irina RIJAMADZE.. 93 Determination of the efficacy of the Bacillus thuringiensis and Neemazal Using in Microbial and Organic control on Pristiphora abietina (Christ) (Hymenoptera: Tenthredinidae) Nurcan ALBAYRAK ĠSKENDER, YaĢar AKSU, Berna ÇELĠK GÖKTÜRK, Cihangir DEDEAĞAOĞLU A Study on Bacterial Flora of Spruce Spider Mite Oligonychus ununguis (Jacobi) (Acari: Tetranychidae) Nurcan ALBAYRAK ĠSKENDER, YaĢar AKSU

16 Pathogenicity on The Pest of Bacteria Isolated from Pristiphora abietina (Christ) (Hymenoptera : Tenthredinidae) Nurcan ALBAYRAK ĠSKENDER, Serkan ÖRTÜCÜ, AyĢegül SARAL, YaĢar AKSU. 96 Assessment of Beauveria bassiana Strains Isolated From Various Insects to Control Dendroctonus micans (Kugel.) Larvae Nurcan ALBAYRAK ĠSKENDER, Serkan ÖRTÜCÜ, Engin KILIÇ, YaĢar AKSU

17 ORAL PRESENTATIONS 17

18 S-01 Functional analysis of AMEV serine/threonin protein kinase gene and its role in host specificity Hacer MURATOĞLU a, Duygu BEKİRCAN b, Remziye NALÇACIOĞLU b, Srini PERERA c, Basil M. ARIF c, Zihni DEMİRBAĞ b a Karadeniz Technical University, Faculty of Science, Department of Molecular Biology and Genetics, Trabzon, Turkey b Karadeniz Technical University, Faculty of Science, Department of Biology, Trabzon, Turkey zihni@ktu.edu.tr c Laboratory for Molecular Virology, Great Lakes Forestry Centre, Sault Ste. Marie, Ontario, Canada Amsacta moorei entomopoxvirus (AMEV) is an insect virus with a potential as an important biotechnological tool. AMEV contains a non-structural serine/threonine (Ser/Thr) protein kinase gene (ORF AMV197), which is a homologue of poxvirus B1 protein kinase and is important for viral DNA replication, gene expression and intermediate gene transcription. Therefore, it is believed to play key role in viral replication. In the current study, protein kinase gene (pk), AMV197 has been deleted from AMEV genome by homologous recombination. DNA replication, protein synthesis, production of infectious progeny virus and transcription of viral genes of parental virus were compared to AMV197-null recombinant virus (AmΔPK/gfp) in cell culture. In AmΔPK/gfp infected Ld652 cells, DNA replication started 6 hour earlier than in cells infected with the parental virus. End Point Dilution Assays indicated that AMV197 deletion caused four fold decreases in the production of progeny virus in cell culture. SDS-PAGE analysis showed that deletion of AMV197 does not appear to cause discernible changes in viral protein profile. Microarray analysis of viral gene expression revealed significant differences between viral gene expression in AMEV and AmΔPK/gfp infected cells. Taken collectively, the data indicate that the protein encoded by AMV197 may have significant effects on the assembly and/or infectivity progeny virus and AMEV s host specificity. However, further investigations are needed to verify the exact role of this gene and effect on host specificity. Key words: AmΔPK/gfp, Amsacta moorei Entomopoxvirus (AMEV), protein kinase, virus replication, RNA microarray, host specificity, gene regulation. Acknowledgement: This study has been funded by The Scientific and Technological Research Council of Turkey (Project No: 110T887) and Karadeniz Technical University, Scientific Research Fund (Project No: 8646). 18

19 S-02 Use of Insect Microflora in Biotechnological Studies Ömer Faruk ALGUR Atatürk University, Faculty of Science, Department of Biology, Erzurum Due to their diversity and widespread distribution, insects are inevitably associated with an extremetily large variety of microscobic life forms including viruses, bacteria, fungi and protozoa. Especially, insect gut is a reservoir of complex microbial communities. For this reason, investigations on using of microbial flora of insect body for biotechnologial applications are gradually increasing. This study gives an overview of diversity and biotechnological potentials of enzymes of microflora of insect. In addition, the biotechnological applications such as biodegradation of harmful materials and production of fuel and bioactive materials, were also presented and discussed under the light of literature. 19

20 S-03 Biological Control of Pest Insects in Germany - Presence and Future Johannes A. JEHLE Institute for Biological Control, Federal Research Center for Cultivated Plants, Julius Kühn Insitute (JKI), Heinrichstr. 243, Darmstadt, Germany johannes.jehle@jki.bund.de The use of biological control methods offers a plentitude of approaches for a highly selective and environmentally sound control of insect pests. These include the application of (i) beneficial arthropods (predators, parasitoids and entomopathogenic nematodes), (ii) entomopathogenic micro-organisms (including fungi, bacteria and viruses), and (iii) natural substances and botanicals for pest control. Biological control methods are highly specific and are considered to be not harmful to human and animal health they are of great importance for Integrated Pest Management Strategies (IPM). There are many examples (predatory mites, Trichogramma, B. thuringiensis, Baculoviruses, Mating disruption), where biological control strategies demonstrated a superior efficacy in greenhouse and field crops. Although there was a considerable increase of biological control in many crops during the last decade, the area-wide application of biological control measures is still hampered by a number of factors. These include the lack of availability of biological control products on the market, the concerns of growers about reliability and costs, and the lack of compatibility with chemical pesticides in some cases. During the last two years a survey on the current use of biological control measures was performed in Germany. The results will be presented together an estimation of their future potential. Key words: Biological control, insect pathogens, EU legislation 20

21 S-04 Codling moth control by Cydia pomonella granulovirus in organic apple orchards: lessons from the differences Mohammadreza REZAPANAH Center of Excellence for Organic Agriculture, Iranian Research Institute of Plant Protection, P.O. Box: , Velenjak, Tehran, Iran The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) with 1-4 generations in different regions causes less than 5% till full damages in apple orchards. High pressure of codling moth poplation expresses extreme potential of its control measures and even their combinations. While the LC 50 of Cydia pomonella granulovirus (CpGV) preparations in laboratory bioassays have been estimated around 1000 GV/g, 50 GV/μl and 20 GV/mm 2 after about 6 days; and, it is usually recommended to covering the apple orchards by around GV/ha or 1000 ( ) GV/mm 2. But, the evaluated control potential of CpGV in regions with 3 or 4 generations of this serious pest are varied and discussable. Of course, organic apple orchards with 1 or 2 generations, not only have hopefully passed past decade by further developing of CpGV preparations via cooperation, but also cured the problems of low susceptibility to the CpGV via the lessons from the minor differences of CpGV isolates. Key words: Codling moth, Cydia pomonella granulovirus, CpGV, organic, apple 21

22 S-05 Biological effects of novel commercial formulations developed for mosquito control Fikrettin ŞAHİN a, M. Muge YAZICI a, Huseyin ÇETİN b, Yusuf KAYA a a Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Kayisdagi-Istanbul, Turkey fsahin@yeditepe.edu.tr b Akdeniz University, Faculty of Science, Department of Biology, Antalya, Turkey Mosquitoes are vectors of many parasite and virus-borne diseases for mammalian systems. Therefore, control of mosquitoes is very important. Usually synthetic chemical products are being used against mosquitoes. These chemicals are costly, have short-term effective and cause environmental pollutions. Extensive use of these chemicals creates so many human and environmental health problems. Therefore, the best method for management of mosquitoes is known to be biological control. The most commonly utilized agents for biological control of mosquitoes are Bacillus species. In the present study, three strain (MBI 5, 6, 7) of Bacillus sphaericus have been isolated and identified as potential larvacide against mosquito species in the genera of Culex spp. and Anopheles spp. Then, three commercial products (granule, wettable powder and liquid formulations) have been developed and tested for their bioactivities in laboratory and field conditions in the vicinity of Istanbul municipality and Mediterranean region. The commercial products found to be successful in laboratory and field studies for their potential performance on different populations of mosquito species in various environmental conditions. Key words: Bacillus sphaericus, Anopheles, Culex, biological control, formulation 22

23 S-06 New insights into the mode of action of Bacillus thuringiensis Vip3A insecticidal proteins Juan FERRÉ, Maissa CHAKROUN, Yolanda BEL, Núria BANYULS, Baltasar ESCRICHE University of Valencia, Faculty of Biology, Department of Genetics, Valencia (SPAIN) Vip3 toxins are Bacillus thuringiensis (Bt) proteins which are secreted to the medium during the vegetative growth and, for this reason they do not contribute to the insecticidal activity of bioinsectices prepared from Bt spore/crystal mixtures. Nevertheless, some of the Vip3 proteins are highly active against Lepidoptera and show a relatively broad insecticidal spectrum. Because Vip3 proteins share no homology with the Bt crystal (Cry) proteins and because they have different modes of action, Vip3A proteins have been combined with Cry1 proteins in transgenic crops (Bt crops). The mode of action of Vip3 proteins is not well understood. It is known that there are some insect species susceptible to Cry1 proteins which are practically completely tolerant to Vip3 proteins and vice versa. Although Vip3 proteins and Cry1 proteins share general features in their modes of action, they differ notably when the different steps are studied in detail. For example, we have found that, contrarily to the Cry1 proteins, Vip3 proteins do not have a protease-resistant core, or at least not so resistant as in the case of Cry1 proteins. This implies that the activation step within the insect s midgut is more critical in the case of Vip3 proteins because there is a dynamic equilibrium between protoxin activation and toxin degradation. A second differential feature between Cry1 and Vip3 proteins is that they bind to different membrane binding sites. This has been shown with different insect species using either labeled Cry1 proteins or labeled Vip3 proteins, and performing competition studies with unlabeled heterologous competitors. This feature means that, for resistant management purposes, it is a good strategy to combine cry1 and vip3 genes in the same plant because mutations in insect populations conferring resistance to one toxin (due to alteration of its receptors) would not confer cross-resistance to the other toxin. Key words: Spodoptera frugiperda, vegetative insecticidal proteins, binding to midgut receptors Acknowledgements: This research was supported by the Spanish Ministries of Science and Innovation (grant ref. AGL C02-01) and of Economy and Competivity (grant ref. AGL C02-01), by grants ACOMP/2009/313 and PROMETEO 2011/044 from the Generalitat Valenciana, and by European FEDER funds. 23