IICT (Tropical research Institute. Lisbon) Coordinating Principal Researcher, from 2003 to present time.

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1 Luis Granger Alfaro Cardoso Professional CV 1. Identification and Academic Education Name Luis Granger Alfaro Cardoso Born in Maputo, 4/7/49 Education Primary, high school and the first 4 university years in Mozambique. Pos graduation 1973/75 University of Cambridge, UK. Graduation 1973 Veterinary Medicine, Technical University of Lisbon. PhD Technical University of Lisbon, Faculty of Veterinary Medicine. 2. Professional positions IICT (Tropical research Institute. Lisbon) Coordinating Principal Researcher, from 2003 to present time. IICT- Director of the Animal Health and Production Unit From September 1999 to present time. IICT Chairman of the Scientific Council. From June 2004 to Fev and from March 2008 to present time. Chairman for the Fórum of Portuguese State Laboratories Scientific Councils From June 2008 to present time. Member of the Stearing Committee of the program "Livestock and Environmental Development" (LEAD),funded by the UE and Worl Bank. From 2002 to 2007 Chairman for the ONGD Associação Tropical Agrária From 2004 to present time. Chairman of the Stearing Committee of the Association of Institutes for Tropical Veterinary Medicine (AITVM), Member of the Stearing Committee of the Association of Institutes for Tropical Veterinary Medicine (AITVM) from Member of the EU ACP Livestock Committee (DGD). From 1995 to present time. Trainee researcher, Assistant researcher, Principal researcher within the Tropical Research Institute IICT) respectively from 1987, 1989, and Private veterinary practice Portugal, from 1984 to 1987

2 Director of agriculture and livestock farms in Mozambique From 1976 to 1984 Post graduate Student at Cambridge University, UK from 1973 to Research activity Research area - tropical livestock production concerning genetic and physiological adaptation of livestock and forrage production to tropical environment. Key words Physiology adaptation livestock forages tropical environment. Main peer reviewed scientific publications - Cardoso, L.A Relacionamento de alguns parâmetros macro económicos e de tecnologia agrária com níveis de produção e de consumo de energia alimentar, em países em diferentes fases de desenvolvimento. Comunicações, IICT Série de Ciências Agrárias, 2: Cardoso, L.A Agrarian research cooperation between european and developing countries institutes". Higher Education Policy Journal of the International Association of Universities 1: UCLA Ed. - Cardoso, L.A. and M.J. Stock Effect of clenbuterol on growth and body composition during food restriction in rats. J. Anim. Sci., 74: Cardoso, L.A. and M.J. Stock Effect of clenbuterol on endocrine status and nitrogen and energy balance in food resticted rats. J. Anim. Sci., : Almeida A.M., L.M. Schwalbach, H.O. Dewall, J.P.C. Greyling, L.A. Cardoso, L.A. dubruin and T. Muller Plasmatic concentratios of insulin, T3 e T4 in Boer goats submittes to food restriction. Israel Journal of Veterinary Medicine 57(4): , -Almeida A.M. and L.A. Cardoso Possible role of antibodies to leptin as growth promoters-preliminary study in mice. Scandinavian Journal of Laboratory Animal Science, 29: Cardoso L.A. and M.L. Ferreira Protective effect of clenbuterol on duodenal epithelium during food restriction in rats. J. South African Vet. Ass., 73 (2): Cardoso L.A. and O. Taveira Effect of clenbuterol on growth, nitrogen and energy balances and endocrine status in food restricted sheep. J. South African Vet. Ass., 73(3):

3 - C. Lavrador, A. J. Almeida e L.A. Cardoso Avaliação in vitro de um injectável subcutâneo para libertação prolongada de um fármaco β-agonista. Revista Portuguesa de Ciências Veterinárias, 97 (541): Almeida A.M. and L.A Cardoso A contribution to the evaluation of agriculture cooperation towards Southern African countries. Journal of Southern African Development, 3:1. - Almeida A.M. S. Van Harten and L.A. Cardoso Serum aminoacids and miofibrillar protein profiles in fed and under fed rats. Nutrition Research, (22): Van Harten, S., A.M. Almeida, Z. Morais and L.A. Cardoso (2003). Free fatty acids and fatty acids of triacylglycerols profiles in muscle and plasma of fed and underfed laboratory rats. Nutrition Research 23: Van Harten, S., A.M. Almeida, Z. Morais, L.M. Schwalbach, J.P. Greyling, H.O. de Waal and L.A. Cardoso (2003). Free fatty acids and fatty acids of triacylglycerols profiles in muscle and plasma of fed and underfed Boer goats. Nutrition Research 23: Almeida, A.M., L.M. Schwalbach, H.O. dewaal, J.P.C. Greyling and L.A. Cardoso (2004). Serum amino acid and myofibrillar protein profiles in Boer Goat bucks following undernutrition. Small Ruminant Research 55: Almeida, A.M., E. Villalobos, S.S. Araújo, B. Leyman, P. Van Dijck, L.A. Cardoso, P.S. Fevereiro, J.M. Torné and D.M. Santos (2005). Transformation of tobacco with an Arabidopsis thaliana gene involved in trehalose biosynthesis increases tolerance to several abiotic stresses. Euphytica 146: Almeida, A.M., L.M. Schwalbach, H.O. de Waal, J.P. Greyling and L.A. Cardoso (2006). The effect of supplementation on productive performance of Boer goat bucks fed winter veld hay. Tropical Animal Health and Production. 38: Almeida, A.M., M. Santos, E. Villalobos, S.S. Araújo, P. van Dijck, B. Leyman, L.A. Cardoso, D. Santos, P.S. Fevereiro and J.M. Torné (2007). Immunogold localization study of trehalose-6-phosphate synthase in leaf segments of wild type and transgenic tobacco plants expressing the AtTPS1 gene from Arabidopsis thaliana. Protoplasma 230: Almeida, A.M., S. Van Harten and L. A. Cardoso (2006). Myofibrillar Protein Status of the Gastrocnemius in Male Rats: Effect of Mild Undernutrition. Scandinavian Journal of Laboratorial Science Vol 33: A. M. Almeida, A. B. Silva, S. S. Araújo, L. A. Cardoso, D. M. Santos, J. Torné, J. M. Silva, M. J. Paul, P. Fevereiro. (2006) Responses to water withdrawal of tobacco plants

4 genetically engineered with AtTPS1 gene: a special reference to photosynthetic parameters. Euphytica DOI /s A.M. Almeida, L. A. Cardoso, Dulce M. Santos, José M. Torné, Manuel P. Fevereiro (2007) Trehalose and its applicatinos in plant biotechnology. In Vitro Cell. Dev. Biol.. Accepted for publication. In vitro Cell Dev. Biol. Plants 43: Almeida, A.M., L.M.J. Schwalbach, L.A. Cardoso and J.P.C. Greyling (2007). Scrotal, testicular and semen characteristics of young Boer bucks fed winter veld hay: the effect of nutritional supplementation. Small Ruminant Research 73: Almeida, A.M., L.A. Cardoso, D.M. Santos, J.M. Torné and P.S. Fevereiro (2007). Invited Review: Trehalose and its applications in plant biotechnology. In vitro Cell Dev. Biol. Plants 43: Almeida, A.M., M. Santos, E. Villalobos, S.S. Araújo, P. van Dijck, B. Leyman, L.A. Cardoso, D. Santos P.S. Fevereiro and J.M. Torné (2007). Immunogold localization study of trehalose-6-phosphate synthase in leaf segments of wild type and transgenic tobacco plants expressing the AtTPS1 gene from Arabidopsis thaliana. Protoplasma 230: Almeida, A.M., E. Villalobos, S.S. Araújo, L.A. Cardoso, D.M. Santos, M. Santos, P.S. Fevereiro and J.M. Torné (2007). Electroporation of maize embryogenic calli with the trehalose-6-phosphate synthase gene from Arabidopsis thaliana. Acta Physiologiae Plantarum 29: A.M. Almeida, A.B. Silva, S.S. Araújo, L.A. Cardoso, D.M. Santos, J.M. Torné, J.M. Silva, M.J. Paul and P.S. Fevereiro (2007). Responses to water withdrawal of tobacco plants genetically engineered with the AtTPS1 gene: a special reference to photosynthetic parameters. Euphytica 154: Almeida, A.M., M. Santos, E. Villalobos, S.S. Araújo, P. van Dijck, B. Leyman, L.A. Cardoso, D. Santos, P.S. Fevereiro and J.M. Torné (2007). Immunolocalization of trehalose-6-phosphate synthase in leaf segments of genetically engineered tobacco plants expressing the AtTPS1 gene. Comparative Biochemistry and Physiology A 146: S272 (doi: /j.cbpa ). - Almeida, A.M. and L.A. Cardoso (2008). Animal Production and Genetic Resources in Guinea Bissau: I Northern Cacheu Province. Tropical Animal Health and Production 40: Almeida, A.M. and L.A. Cardoso (2008). Animal Production and Genetic Resources in Guinea Bissau: II Tombali Province. Tropical Animal Health and Production 40:

5 - Almeida, A.M., L.M. Schwalbach, H.O. dewaal, J.P. Greyling and L.A. Cardoso (2008). 3-Methyhistidine in the male goat as an experimental model: serum concentrations and an indicator of protein breakdown. Scandinavian Journal of Laboratory Animal Science (In press) - Almeida, A.M., S. van Harten, A. Campos, A. Varela and L.A. Cardoso (2008). Weight loss and protein expression profiles in the gastrocnemius muscle of two rabbit breeds. Proteómica (Córdoba, Spain) 1: Carvalho M., Mateus L., Afonso F., Van Harten S., L. A. Cardoso, Redmer D.A., Ferreira-Dias, G Testicular Angiogenic Activity in Response to Food Restriction in Rabbit. Reproduction 137: A. Almeida, A. Campos, S, V. Harten, Cardoso L.A. and A. V. Coelho Establishment of a proteomic reference map for the gastrocnemius muscle in the rabbit (Oryctolagus cuniculus). Research in Veterinary Science. In press. Book Chapters - Almeida, A.M., S. van Harten e L.A. Cardoso (2005). Food restriction effects on Plasma amino acids, myofibrillar protein profiles, plasma and muscle free and sterified fatty acids on monogastric and ruminants: A Review. Focus on Nutrition Research. Editado por T. Starks, Nova Publishers (New York, E.U.A.). - Almeida, A.M., L.A. Cardoso, D. Santos, J.M. Torné e M.P. Fevereiro (2006). The increase of water deficit tolerance in plants through genetic engineering of the trehalose metabolism. Genetic Resources and Biotechnology IV. Editado por R. Thangadurai, Regency Publications (New Delhi, India) 4. Development programs or projects coordinated or participated by the candidate Mozambique. Elaboration and coordinating the implementation of the Projecto de Formação profissional Agrária, Mumemo, Mozambique Funded by IPAD (Instituto Português de Apoio ao Desenvolvimento). Angola. Elaboration and coordinating the implementation of the Projecto de Desenvolvimento da Comuna de Ekunha, Huambo, Angola Funded by the EU and IPAD.

6 Guiné Bissau. Elaboration and coordinating the implementation of the Projecto de Desenvolvimento Pecuário da região de Komkubai, Guiné Bissau Funded by the EU and IPAD. Angola Participating (in Angola) in the leadership of the team responsible for the elaboration of the Plano Director das Bacias Leiteiras de Angola (Diary Production Director Plan, Angola) Funded by the MINADER, (Ministery of Agriculture and Rural Development) Angola. Senegal, Gambia, Burkina Faso,Mali, Ghana, Togo, Nigéria, Camarões. Evaluation of thewest Africa Agriculture Research Network (CORAF) Funded by ECART. Mozambique. Elaboration and coordinating the implementation of the Projecto de Desenvolvimento Agrário de Mumemo, Provínce of Maputo, Mozambique Funded by IPAD, Portugal Angola. Local study of the "Programa Integrado de Desenvolvimento Rural da Província do Cunene" Funded by IICT (Tropical Research Institute, Portugal). S. Tomé e Príncipe. Project for Livestock Development in S. Tomé e Príncipe. Mission on livestock nutrition. Funded by BAD (African Development Bank) Cabo-Verde. Elaboration and coordinating the implementation of the "Projecto de Avicultura e Cunicultura de Santiago, Cabo-Verde" (Poultry and rabbit Project in Santiago, Cabo Verde) Funded by IPAD. Zimbabwé. Participation in the Project Natural Resources Management Within Multispecies System in the Mid-Zambezi Valley: Implcations for Sustainable Developmant in Dry Lands Areas of Southern Africa. Funded by UE Guiné-Bissau. Elaboration and coordinating the implementation of the Projecto de Implantação e Ensaio de Forrageiras Leguminosas Tropicais para Alimentação de Coelhos na Guiné-Bissau Funded by IICT Guiné-Bissau. Elaboration and coordinating the implementation of the Projecto de Cunicultura e Avicultura para a Região de Cacheu, Guiné-Bissau Funded by IICT Mozambique. Elaboration Projecto de Reabilitação do Efectivo e da Produção Leiteira da Província de Maputo, (Maputo Province dairy cattle reabilitation Project) Funded by IPAD.. Mozambique. Elaboration and coordinating the implementation of the Projecto de Inseminação Artificial para a Produção de Gado Leiteiro na Província de Maputo (Artificial Insemination Project for Dairy Cattle in Maputo Province) Funded by IPAD.

7 Guiné-Bissau. Elaboration and coordinating the implementation of the Projecto de Cunicultura e Avicultura para a Cintura Residencial de Bissau. (Poultry and rabbitt production development Project for the city of Bissau), Funded by IPAD. Mozambique. Elaboration of the "Projecto reabilitacão da producão pecuária na área de regadio do Chokwé, Limpopo" (Livestock reabilitaion Project for the irrigated Chókwé region, Limpopo). República de Mozambique. Project requested by the Secretaria de Estado da Hidráulica Agrícola da República de Mozambique Funded by IPAD Guiné-Bissau. Elaboration of the "Projecto de combate à desertificação das zonas de pastoreio da Região de Oio, Guiné-Bissau". Project requested by the Departamento de Pesquisa Agrária do Ministério da Agricultura da Guiné-Bissau Funded by IICT Guiné-Bissau. Elaboration of the "Projecto de recuperação de solos salinizados na região de Oio, Guiné-Bissau". Project requested by the Departamento de Pesquisa Agrária do Ministério da Agricultura da Guiné-Bissau Funded by IICT. Mozambique. Elaboration of the "Projecto técnico de uma unidade de produção de biogás através da digestão biológica do excreta de galinha". Project requested by the Secretaria de Estado da Hidráulica Agrícola da República de Mozambique Portugal. Patent registration, in the name of the candidate, of the "Equipamento móvel de tratamento químico e fisico de palha e de outros produtos lenhosos" (Mobile equipment for chemical and physical teatment of lignocellulose products) Mozambique - Head of Agro-Industrial Unit within SERLI, (Secretaria de Estado do Desenvolvimento do Limpopo e lncomáti) Mozambique. Coordinating the work of the directors and multidisciplinary teams of experts the of each of agriculture and livestock farms and agro-industrial plants, (tomato industry) including about 70% of the country's rice production, 15% of milk production and beef cattle heads. The work included investment plans conception and farms perfomance evaluation. The work was personally supervized by the Secretary of State for the Development of Limpopo and Incomati Valley. l Mozambique - Director of the Empresa Estatal de Gado de Corte do Sul. Organizing and managing the "Empresa Estatal de Gado de Corte do Sul", within the southern provincies of Maputo and Gaza provincies of Mozambique, including beff cattle heads. The work included planning and implementing new production units or the reform of old ones so as to achieve sound productivity levels which represented a significative component of Maputo city meat suply

8 5. University Teaching In charge for the course Food Production within the Instituto Superior de Ciências da Saúde From 1993 to In charge for the Physiology course within the Instituto Superior de Ciências da Saúde From 2000 to Participating in the Livestock Production Course within the MSc diploma in Tropical Animal Production of the Faculty of Veterinary Medicine (Technical University of Lisbon) From 1993 to present time.

2007: PhD degree in Biology, Instituto de Tecnologia Química e Biológica, New University of Lisbon, Portugal (approved by unanimity)

2007: PhD degree in Biology, Instituto de Tecnologia Química e Biológica, New University of Lisbon, Portugal (approved by unanimity) 1 - Personal data Susana de Sousa Araújo Birth date: 29-08-1976 Nationality: Portuguese Institute address: Centro de Veterinária e Zootecnia do IICT Faculdade de Medicina Veterinária Avenida da Universidade

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