Replacement Instructions ABI 431A/433A Waste Port

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1 Replacement Instructions ABI 431A/433A Waste Port February 1996 (updated 09/2002) IMPORTANT Do not use the installation instructions contained within the waste port assembly packaging because they are incomplete. Use these instructions ahead. Before You Start You need the following equipment: 13/16-inch open-end wrench or adjustable wrench utility knife or new single-edged razor blade 2 inches of Teflon tape WARNING CHEMICAL HAZARD. The tubes in and around the waste port contain dangerous liquids that can damage your eyes and skin. Always wear protective lab coat, gloves, and safety goggles when handling tubes that may contain even small amounts of reagents such as N-methylpyrrolidone (NMP) or trifluoroacetic acid (TFA). Kit Contents The kit consists of: waste port fitting assembly (P/N ) installation instructions (P/N ) letter

2 Preparing the ABI 431/433A Before you remove the existing waste port, make sure the ABI 431/433A is idle. Removing the Existing Waste Port Remove the existing waste port assembly using the following steps: 1. Loosen the nut nearest the waste tube by turning the nut with your fingers or with the 13/16-inch wrench (Figure 1). 2. Remove the nut from the fitting body. rear of the ABI 431/433A loosen this nut waste tube fitting body Figure 1. Waste port view at rear of ABI 431/433A 3. Remove the nut from the waste tube. 4. Set the nut aside and lay the waste tube on the table top. Page 2 of 8

3 5. Look inside the fitting body still attached to the ABI 431/433A. Note that you can see seven small feeder tubes that drain into the waste tube (Figure 2). It will be important to install the new fitting body so that these seven feeder tubes are not crimped, bent, or otherwise displaced from their present position. fitting body nut 7 small tubes end view side view showing hidden feeder tubes Figure 2. Waste port feeder tubes 6. Using the 13/16-inch wrench, remove the fitting body from the ABI 431/433A. 7. Discard the old fitting body and nut in accordance with all local, state, and federal hazardous waste regulations. Page 3 of 8

4 Install the New Waste Port Fitting Body To install the new waste port fitting body: 1. Remove the new waste port fitting assembly from the plastic packaging. 2. Carefully separate the fitting body from the nut. The ferrule and gripper are located inside the assembly and can be easily lost if dropped. 3. Wrap the Teflon tape around the threads of the wider end of the fitting body (Figure 3). Make sure you wrap the tape in the direction shown in the figure. wider end wrap Teflon tape in this direction Figure 3. Fitting body showing direction to wrap Teflon tape Caution Read the entire next step before proceeding. 4. Thread the wide end of the fitting body (the end with the Teflon tape) into the waste port aperture on the back of the ABI 431/433A by hand, taking care not to bend or crimp any of the seven small feeder tubes. As you thread the fitting body, look inside to make sure you see all seven of the small tubes you observed in step 5. Thread the fitting body into the aperture by hand until fully seated. 5. After the fitting body is fully seated by hand, use the wrench to tighten the fitting body 1/2 turn more. Page 4 of 8

5 Installing the New Waste Port Nut/Ferrule Assembly To install the new waste port nut/ferrule assembly: IMPORTANT You must use a perpendicular cut when you remove the piece from the end of the waste tube. If the end is not perpendicular, a leak may develop at the fitting body. 1. Using the utility knife or razor blade, cut off a short piece of waste tube to remove any indentation, discoloration, or degradation. Discard the short piece. 2. The new waste port is one of two different assemblies that have two varieties of ferrule and gripper. Identify which assembly you have received by comparing the ferrule and gripper with the illustrations shown in Figure If you have a new style A waste port, remove the gripper from the waste port nut (Figure 4). You can use a pair of needle-nose pliers to push or pull the gripper from inside the nut. Figure 4. Waste port fitting assembly 4. Insert the newly-cut end of the waste tube through the non-threaded end of the nut (Style A has a nut on that end) so that several inches of waste tube protrudes through the nut. 5. Slide the gripper onto the waste tube (Figure 4). Page 5 of 8

6 6. Slide the ferrule onto the waste tube with the wide shoulder of the ferrule going on first. The shoulder of the ferrule should rest evenly against the gripper. 7. Insert the waste tube into the fitting body until the internal tube stop is reached (Figure 5). 8. Slide the ferrule and gripper up against the fitting body (Figure 5). Figure 5. Fitting body with ferrule and gripper (Style A) 9. Start threading the nut to the fitting by hand. Ensure that you completely insert the waste tube so it can travel no further into the fitting. Caution Do not use Teflon tape between the nut and the fitting. This can cause the ferrule to seat incorrectly and leak. 10. Thread the nut onto the fitting by hand until you cannot turn the nut any further. 11. If you are installing a style A waste port, use the 13/16-inch wrench to tighten the nut 1/2 turn more. Do not tighten the style B waste port any further now. If the waste port leaks, tighten the waste port 1/2 turn using channel-lock pliers. 12. Make sure the waste line is long enough and positioned so that it is not under tension or severely pulled by routine handling of the waste bottle or instrument. Be sure the line is not so long that dips are created where liquid could collect. Check the waste port for damage every time you empty the waste bottle. Replace the port if it shows any indication of breaking, leaking, or is heavily discolored. Page 6 of 8

7

8 Copyright 2002, Applied Biosystems For Research Use Only. Not for use in diagnostic procedures. ABI PRISM and Applied Biosystems are registered trademarks, and ABI is a trademark of Applera Corporation or its subsidiaries in the U.S. and certain other countries. All other trademarks are the sole property of their respective owners. P/N B

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