The 44 th Congress on Science and Technology of Thailand (STT44)

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3 ทรงพระเจร ญ

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5 ABSTRACTS BOOK The 44 th Congress on Science and Technology of Thailand (STT44) Science and Technology in the Disruptive Era October 29-31, 2018 Bangkok International Trade & Exhibition Centre (BITEC) Thailand Organized by: The Science Society of Thailand under the Patronage of His Majesty the King

6 บทค ดย อ การประช มว ชาการว ทยาศาสตร และเทคโนโลย แห งประเทศไทย คร งท 44 ISBN จ ดทาโดย สมาคมว ทยาศาสตร แห งประเทศไทยในพระบรมราช ปถ มภ และม ลน ธ เทคโนโลย สารสนเทศตามพระราชดาร สมเด จพระเทพร ตนราชส ดา ฯ สยามบรมราชก มาร พ มพ คร งแรก ต ลาคม พ.ศ สงวนล ขส ทธ ตามพระราชบ ญญ ต การพ มพ โดยสมาคมว ทยาศาสตร แห งประเทศไทยในพระบรมราช ปถ มภ ไม อน ญาตให ค ดลอกส วนหน งส วนใดของหน งส อเล มน นอกจากได ร บอน ญาตจากเจ าของล ขส ทธ ก อนเท าน น

7 CONTENTS Message from the President of the Science Society of Thailand under the Patronage of His Majesty the King 3 Report from the Chairperson of STT44 Organizing Committee 4 History of the Congress on Science and Technology of Thailand 5 Program Overview 8 Maps 9 Program for Grand Opening Ceremony of STT44 11 Abstract of Honorable Keynote Speaker Her Royal Highness Princess Maha Chakri Sirindhorn Abstract of Plenary Speaker Dr. Huigen Yang, Former Chair of the Asian Forum for Polar Sciences, Director General of Polar Research Institute of China, People Republic of China Abstract of Plenary Speaker Dr. Branimir Jovanovic, Nikola Tesla Museum, Belgrade, Serbia Abstract of Plenary Speaker Professor Orawon Chailapakul, 2018 Thailand Outstanding Scientist Program for Special Sessions, Meetings, Forums, Invited Lectures and Oral Presentations 29 th October th October st October Abstracts for Special Sessions and Invited Lectures 38 Poster Sessions 89 1 The 44 th Congress on Science and Technology of Thailand (STT 44)

8 CONTENTS SESSION A: AGRICULTURAL SCIENCE / BIOTECHNOLOGY 101 SESSION B: BIOLOGICAL SCIENCE (B1: BIOCHEMISTRY) 133 SESSION B: BIOLOGICAL SCIENCE (B2: MICROBIOLOGY) 153 SESSION B: BIOLOGICAL SCIENCE (B3: MOLECULAR BIOLOGY) 175 SESSION B: BIOLOGICAL SCIENCE (B4: BIOMEDICAL SCIENCE) 185 SESSION B: BIOLOGICAL SCIENCE (B5: BIODIVERSITY) 193 SESSION C: CHEMISTRY (C1: ANALYTICAL CHEMISTRY) 210 SESSION C: CHEMISTRY (C2: INORGANIC CHEMISTRY) 223 SESSION C: CHEMISTRY (C3: ORGANIC & MEDICINAL CHEMISTRY) 229 SESSION C: CHEMISTRY (C4: PHYSICAL & THEORETICAL CHEMISTRY) 242 SESSION D: POLYMER & MATERIALS SCIENCE / NANOTECHNOLOGY 251 SESSION E: ENERGY / ENVIRONMENTAL& EARTH SCIENCE 279 SESSION F: PHYSICS / APPLIED PHYSICS 292 SESSION G: MATHEMATICS / STATISTICS / COMPUTER SCIENCE 301 SESSION H: FOOD SCIENCE & TECHNOLOGY 304 SESSION SP4: CRYSTALLOGRAPHY 326 Organizing Committee Of 44 th Congress On Science 349 And Technology Of Thailand Acknowledgement For The Sponsors Of STT Exhibition Booths 351 Author Index Science and Technology in the Disruptive Era

9 MESSAGE FROM THE PRESIDENT OF THE SCIENCE SOCIETY OF THAILAND UNDER THE PATRONAGE OF HIS MAJESTY THE KING The Congress on Science and Technology of Thailand (STT) is the largest national annual science conference in Thailand, organized by the Science Society of Thailand under the Patronage of His Majesty the King since Every year, each of the 28 wellknown public universities in Thailand, both in Bangkok and in the provinces, takes turn in co-hosting this prestigious conference. And in the year 2018, the STT 44 Conference is solely hosted by the Science Society of Thailand (SST) due to two special occasions : Firstly, to commemorate the 25th Anniversary of Her Royal Highness Princess Maha Chakri Sirindhorn for Her visiting the Antarctica and secondly, to celebrate the 70th Anniversary Year of the SST. Hence, the Congress is highlighted by the Honorable Keynote Lecture graciously delivered by Her Royal Highness Princess Maha Chakri Sirindhorn on the Congress Grand Opening of 29th October. On behalf of The Science Society of Thailand, I would like to express my deepest gratitude to Professor Dr. Pairash Tajchayapong, my special thanks to all supports and contributions from various organizations including public sectors, universities and private agencies, to the Chairperson of the Organizing Committee, to all working teams of STT 44, to all distinguished guests and all participants for supporting and contributions to this STT44 Congress on Science and Technology in the Disruptive Era. Associate Professor Dr. Napavarn Noparatnaraporn President The Science Society of Thailand under the Patronage of His Majesty the King 3 The 44 th Congress on Science and Technology of Thailand (STT 44)

10 REPORT FROM THE CHAIRPERSON OF STT44 ORGANIZING COMMITTEE This year the 44 th Congress on Science and Technology of Thailand (STT44) is hosted by the Science Society of Thailand under the Patronage of His Majesty the King (SST) to commemorate the 25 th anniversary of Her Royal Highness Princess Maha Chakri Sirindhorn visiting the Antarctica and to celebrate the 70 th year of the SST. The congress is held during October 29-31, 2018 under the theme of Science and Technology in the Disruptive Era at Bangkok International Trade & Exhibition Centre (BITEC). We are most privileged and honored to have Her Royal Highness Princess Maha Chakri Sirindhorn graciously preside over the Opening Ceremony and give a Keynote Lecture, followed by Professor Yang Huigen, Director General of Polar Research Institute of China, People Republic of China, Dr.Branimir Jovanovic, Director of Nikola Tesla Museum, Serbia, and honorable lecture from the 2018 Outstanding Scientist of Thailand, Professor Orawon Chailapakul, Department of Chemistry, Chulalongkorn University. This year the Congress is arranged into 8 sessions of applied science and technology along with 5 special sessions on Polar Research, How the world (and Thailand) changes in this disruptive era?, Innovative Immunotheraphy of HIV/AIDS and Cancer with Edible Plant Extracts, Crystallography, and zspace 3D Virtual Reality Technology for STEM Learning Lab. Moreover, there are lectures from several renowned invited speakers, panel discussions and hundreds of contributed papers from various areas of Science and Technology presented orally or in the form of posters. The Congress also includes high school science projects that won at national level and those selected to be Best of the Best Projects of the year. Exhibition on advanced scientific and technological knowledge or instruments / appliances from various organizations and a number of suppliers will be displayed. I would like to thank all those who provide consistent support to Science Society of Thailand to promote the highest quality of the STT congress, namely, National Science Museum, National Innovation Agency, Power of Innovation Foundation, Foundation for Promotion of Science and Technology under the Patronage of His Majesty the King, National Science Technology and Innovation Policy Office, National Astronomical Research Institute of Thailand, The Thailand Research Fund, TPI Polene Public Co.,Ltd, Asian Phytoceuticals Public Co.,Ltd, Bara Scientific Co.,Ltd, Bangkok Bank Public Co.,Ltd, The Siam Cement Public Co.,Ltd, Thai Union Group Public Co.,Ltd. This congress will never be possible without help and contribution from many people involved. Firstly, my special thanks go to invited speakers, the Polar research group led by Professor Dr.Pairash Tajchayapong, and all sets of committee of STT44 that help making this congress to international standard. The scientific committee chaired by Associate Professor Dr.Pranut Potiyaraj, co-chaired by Associate Professor Dr.Onruthai Pinyakong are greatly acknowledged. Lastly, I would like to thank the participants for attending and sharing your work at the STT44 congress. Dr.Saiwarun Chaiwanichsiri, Associate Professor Chairperson, STT44 4 Science and Technology in the Disruptive Era

11 ประว ต การประช มว ชาการว ทยาศาสตร และเทคโนโลย แห งประเทศไทย โดยสมาคมว ทยาศาสตร แห งประเทศไทยในพระบรมราช ปถ มภ HISTORY OF THE CONGRESS ON SCIENCE AND TECHNOLOGY OF THAILAND คร งท ป / ว นท เด อน ช อการประช ม สถาบ นเจ าภาพร วม ประธาน (จานวนผลงานว จ ย) 1. พ.ศ การว จ ยทางว ทยาศาสตร กร งเทพฯ คณะว ทยาศาสตร ศ.ดร.ประช มส ข อาชวอาร ง พฤศจ กายน 2. พ.ศ พฤศจ กายน -2 ธ นวาคม 3. พ.ศ ธ นวาคม 4. พ.ศ ธ นวาคม 5. พ.ศ ธ นวาคม 6. พ.ศ ธ นวาคม 7. พ.ศ ธ นวาคม 8. พ.ศ ต ลาคม 9. พ.ศ ต ลาคม 10. พ.ศ ต ลาคม 11. พ.ศ ต ลาคม 12. พ.ศ ต ลาคม 13. พ.ศ ต ลาคม 14. พ.ศ ต ลาคม 15. พ.ศ ต ลาคม 16. พ.ศ ต ลาคม 17. พ.ศ ต ลาคม 18. พ.ศ ต ลาคม 19. พ.ศ ต ลาคม 20. พ.ศ ต ลาคม 21. พ.ศ ต ลาคม 22. พ.ศ ต ลาคม 2514 การว จ ยทางว ทยาศาสตร กร งเทพฯ 2516 การว จ ยทางว ทยาศาสตร กร งเทพฯ 2518 การว จ ยทางว ทยาศาสตร กร งเทพฯ 2520 ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาภาคเหน อ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาประเทศ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาประเทศ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาประเทศ ว ทยาศาสตร และเทคโนโลย เพ อการ พ ฒนาภาคตะว นออกเฉ ยงเหน อ ว ทยาศาสตร และเทคโนโลย แห ง ประเทศไทย ว ทยาศาสตร และเทคโนโลย แห ง ประเทศไทย ว ทยาศาสตร และเทคโนโลย แห ง ประเทศไทย ว ทยาศาสตร และเทคโนโลย แห ง ประเทศไทย ว ทยาศาสตร และเทคโนโลย แห ง ประเทศไทย ว ทยาศาสตร และเทคโนโลย เพ อการ พ ฒนาทร พยากรภาคเหน อ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาประเทศ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาทร พยากรภาคเหน อ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาประเทศ ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาชายฝ ง ว ทยาศาสตร และเทคโนโลย เพ อการ พ ฒนาเศรษฐก จ ส งคม และ ส งแวดล อม ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาอ ตสาหกรรม ว ทยาศาสตร และเทคโนโลย เพ อพ ฒนาทร พยากรมน ษย จ ฬาลงกรณ มหาว ทยาล ย คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย คณะว ทยาศาสตร คณะแพทยศาสตร มหาว ทยาล ยมห ดล คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย คณะว ทยาศาสตร มหาว ทยาล ยเช ยงใหม คณะว ทยาศาสตร มหาว ทยาล ยศร นคร นทรว โรฒ บางแสน คณะว ทยาศาสตร คณะแพทยศาสตร มหาว ทยาล ยมห ดล คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย คณะว ทยาศาสตร มหาว ทยาล ยขอนแก น คณะว ทยาศาสตร มหาว ทยาล ยเช ยงใหม คณะว ทยาศาสตร มหาว ทยาล ยเกษตรศาสตร คณะว ทยาศาสตร มหาว ทยาล ยศร นคร นทรว โรฒ ประสานม ตร คณะว ทยาศาสตร มหาว ทยาล ยสงขลานคร นทร ว ทยาเขตหาดใหญ คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย คณะว ทยาศาสตร มหาว ทยาล ยเช ยงใหม คณะว ทยาศาสตร สถาบ นเทคโนโลย พระจอมเกล า เจ าค ณทหารลาดกระบ ง คณะว ทยาศาสตร มหาว ทยาล ยขอนแก น คณะว ทยาศาสตร มหาว ทยาล ยเกษตรศาสตร ณ ศ นย การประช มแห งชาต ส ร ก ต คณะว ทยาศาสตร มหาว ทยาล ยสงขลานคร นทร ณ โรงแรมด ส ต เจ.บ.หาดใหญ คณะว ทยาศาสตร และเทคโนโลย มหาว ทยาล ยธรรมศาสตร ณ เซ นทร ลพลาซ า คณะว ทยาศาสตร มหาว ทยาล ยบ รพา ณ โรงแรมแอมบาสซาเอร ซ ต จอมเท ยน ชลบ ร คณะว ทยาศาสตร มหาว ทยาล ยรามค าแหง ณ บางกอกคอนเวนช นเซ นเตอร เซ นทร ลพลาซา ลาดพร าว (83 เร อง) ศ.ดร.ประช มส ข อาชวอาร ง (219 เร อง) ศ.ดร.กาจร มน ญป จ (249 เร อง) รศ.ดร.กาจ ด มงคลก ล (344 เร อง) ศ.ดร.พรช ย มาต งคสมบ ต (232 เร อง) ศ.ดร.พรช ย มาต งคสมบ ต (232 เร อง) รศ.ดร.น ยพ น จ คชภ กด (233 เร อง) รศ.ดร.ส ณห พณ ชยก ล (245 เร อง) รศ.ดร.ส ณห พณ ชยก ล (174 เร อง) ศ.ดร.มนตร จ ฬาว ฒนทล (280 เร อง) ศ.ดร.มนตร จ ฬาว ฒนทล (251 เร อง) รศ.ดร.ภ ญโญ พาน ชพ นธ (277 เร อง) รศ.ดร.ภ ญโญ พาน ชพ นธ (420 เร อง) ศ.ดร.จร ยา บรอคเคลแมน (259 เร อง) ศ.ดร.จร ยา บรอคเคลแมน (394 เร อง) ศ.ดร.ว ช ย ร วตระก ล (369 เร อง) ศ.ดร.ว ช ย ร วตระก ล (349 เร อง) ศ.ดร.ส ชาต อ ปถ มภ (297 เร อง) ศ.ดร.ส ชาต อ ปถ มภ (438 เร อง) ศ.ดร.สมศ กด พ นธ ว ฒนา (252 เร อง) ศ.ดร.สมศ กด พ นธ ว ฒนา (354 เร อง) รศ.ดร.พ ณท พ ร นวงษา (333 เร อง) 23. พ.ศ ว ทยาศาสตร และเทคโนโลย คณะว ทยาศาสตร มหาว ทยาล ยเช ยงใหม รศ.ดร.พ ณท พ ร นวงษา 5 The 44 th Congress on Science and Technology of Thailand (STT 44)

12 คร งท ป / ว นท เด อน ช อการประช ม สถาบ นเจ าภาพร วม ประธาน (จานวนผลงานว จ ย) ต ลาคม เพ อพ ฒนาค ณภาพช ว ตในภ ม ภาค ณ โรงแรมโลต ส ปางสวนแก ว (495 เร อง) 24. พ.ศ ต ลาคม ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาเศรษฐก จท ม นคง คณะว ทยาศาสตร มหาว ทยาล ยมห ดล ณ ศ นย การประช มแห งชาต ส ร ก ต ผศ.ดร.ท พาพร ล มปเสน ย (463 เร อง) 25. พ.ศ ต ลาคม ว ทยาศาสตร และเทคโนโลย เพ อการ พ ฒนาทร พยากรท องถ น คณะว ทยาศาสตร มหาว ทยาล ยนเรศวร ณ โรงแรมอมร นทร ลาก น พ ษณ โลก ผศ.ดร.ท พาพร ล มปเสน ย (581 เร อง) 26. พ.ศ ต ลาคม ว ทยาศาสตร และเทคโนโลย ส สห สวรรษใหม คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย ณ ศ นย การประช มแห งชาต ส ร ก ต รศ.ดร.ศ ภวรรณ ต นตยานนท (739 เร อง) 27. พ.ศ ต ลาคม ว ทยาศาสตร และเทคโนโลย เพ อการฟ นฟ เศรษฐก จไทย มหาว ทยาล ยสงขลานคร นทร โรงแรม ล การ เดนส พลาซ า รศ.ดร.ศ ภวรรณ ต นตยานนท (921 เร อง) 28. พ.ศ ต ลาคม ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาเศรษฐก จท ย งย น คณะว ทยาศาสตร ประย กต สถาบ นเทคโนโลย พระจอมเกล าพระนครเหน อ รศ.ดร.ส ร นทร เหล าส ขสถ ตย (834 เร อง) ณ ศ นย การประช มแห งชาต ส ร ก ต 29. พ.ศ ว ทยาศาสตร และเทคโนโลย คณะว ทยาศาสตร มหาว ทยาล ยขอนแก น รศ.ดร.ส ร นทร เหล าส ขสถ ตย ต ลาคม 30. พ.ศ ต ลาคม 31. พ.ศ ต ลาคม 32. พ.ศ ต ลาคม 33. พ.ศ ต ลาคม 34. พ.ศ ต ลาคม-2 พฤศจ กายน 35. พ.ศ ต ลาคม 36. พ.ศ ต ลาคม 37. พ.ศ ต ลาคม 38. พ.ศ ต ลาคม 39. พ.ศ ต ลาคม 40. พ.ศ ธ นวาคม 41. พ.ศ ธ นวาคม 42. พ.ศ พฤศจ กายน 2 ธ นวาคม 43. พ.ศ ต ลาคม เพ อการพ ฒนาท องถ น ว ทยาศาสตร และเทคโนโลย เพ อส งคมและเศรษฐก จฐานความร ว ทยาศาสตร และเทคโนโลย เพ อการพ ฒนาท ย งย น ว ทยาศาสตร และเทคโนโลย เพ อการ เศรษฐก จพอเพ ยง เฉล มฉลองการครองส ร ราชสมบ ต ครบ 60 ป ของพระบาทสมเด จพระ เจ าอย ห ว ว ทยาศาสตร และเทคโนโลย เพ อโลก ย งย น เฉล มฉลองมหามงคล เฉล ม พระชนมพรรษาครบ 80 พรรษา ของ พระบาทสมเด จพระเจ าอย ห ว ว ทยาศาสตร และเทคโนโลย สาหร บโลกแห งความท าทาย ว ทยาศาสตร และเทคโนโลย เพ อ อนาคตท ด ข น ว ทยาศาสตร และเทคโนโลย เพ อส งคม ท ด ข น ว ทยาศาสตร สร างสรรค เพ อสรรค สร างอนาคต ว ทยาศาสตร เพ ออนาคตของมวล มน ษยชาต นว ตกรรมว ทยาศาสตร เพ อช ว ตท ด ข น ว ทยาศาสตร และเทคโนโลย ส ว ถ พ ฒนาอาเซ ยน ประต ส อาเซ ยนด วยว ทยาศาสตร และ เทคโนโลย ศาสตร แห งแผ นด น ส นว ตกรรม เพ อ อนาคตท ย งย น เข าใจว ทยาศาสตร เข าถ งเทคโนโลย สร างนว ตกรรม นาส งคมย งย น ณ ศ นย ประช มอเนกประสงค กาญจนาภ เษก คณะว ทยาศาสตร มหาว ทยาล ยศร นคร นทรว โรฒ ณ ศ นย แสดง ส นค าและการประช มอ มแพ ค เม องทองธาน เทคโนธาน มหาว ทยาล ยเทคโนโลย ส รนาร คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย ศ นย การประช มแห งชาต ส ร ก ต มหาว ทยาล ยวล ยล กษณ จ งหว ดนครศร ธรรมราช คณะว ทยาศาสตร สถาบ นเทคโนโลย พระจอมเกล า เจ าค ณทหารลาดกระบ ง 6 Science and Technology in the Disruptive Era (1039 เร อง) รศ.ดร.ส ร นทร เหล าส ขสถ ตย (854 เร อง) รศ.ดร.ส ร นทร เหล าส ขสถ ตย (1021 เร อง) รศ.ดร.นภาวรรณ นพร ตนราภรณ (927 เร อง) รศ.ดร.นภาวรรณ นพร ตนราภรณ (802 เร อง) รศ.ดร.นภาวรรณ นพร ตนราภรณ (777 เร อง) คณะว ทยาศาสตร มหาว ทยาล ยบ รพา รศ.ดร.นภาวรรณ นพร ตนราภรณ (854 เร อง) คณะว ทยาศาสตร และเทคโนโลย รศ.ดร.ธาราร ตน ศ ภศ ร มหาว ทยาล ยธรรมศาสตร (582 เร อง) คณะว ทยาศาสตร มหาว ทยาล ยมห ดล รศ.ดร.ธาราร ตน ศ ภศ ร (699 เร อง) คณะว ทยาศาสตร มหาว ทยาล ยเช ยงใหม รศ.ดร.ธาราร ตน ศ ภศ ร (690 เร อง) คณะว ทยาศาสตร รศ.ดร.ธาราร ตน ศ ภศ ร มหาว ทยาล ยเทคโนโลย พระจอมเกล าธนบ ร (495 เร อง) คณะว ทยาศาสตร ศ.ดร. เป ยมส ข พงษ สว สด มหาว ทยาล ยขอนแก น (543 เร อง) มหาว ทยาล ยเทคโนโลย ส รนาร ศ.ดร. เป ยมส ข พงษ สว สด (384 เร อง) คณะว ทยาศาสตร มหาว ทยาล ยเกษตรศาสตร ศ.ดร. เป ยมส ข พงษ สว สด ณ เซ นทาราแกรนด แอท เซ นทร ลพลาซา (290 เร อง) ลาดพร าว คณะว ทยาศาสตร จ ฬาลงกรณ มหาว ทยาล ย ณ อาคารจามจ ร 10 จ ฬาลงกรณ มหาว ทยาล ย ศ.ดร. เป ยมส ข พงษ สว สด (327 เร อง)

13 คร งท ป / ว นท เด อน ช อการประช ม สถาบ นเจ าภาพร วม ประธาน (จานวนผลงานว จ ย) 44. พ.ศ ว ทยาศาสตร และเทคโนโลย ในย ค ณ ศ นย น ทรรศการและการประช มไบเทค รศ.ดร.สายวร ฬ ช ยวาน ชศ ร ต ลาคม พล กผ น บางนา 7 The 44 th Congress on Science and Technology of Thailand (STT 44)

14 PROGRAM OVERVIEW 8 Science and Technology in the Disruptive Era

15 MAP OF BITEC 9 The 44 th Congress on Science and Technology of Thailand (STT 44)

16 ACCESS & FLOOR PLAN 10 Science and Technology in the Disruptive Era

17 PROGRAM FOR GRAND OPENING CEREMONY OF STT44 29 th OCTOBER 2018 Time Events 7:00 Registration 8:00 All guests are seated in Grand Hall GH , BITEC 9:00 Arrival of Her Royal Highness Princess Maha Chakri Sirindhorn - Presentation of Souvenirs and Program Book by Associate Professor Dr.Napavarn Noparatnaraporn, President of The Science Society of Thailand under the Patronage of His Majesty the King, and Associate Professor Dr.Pranut Potiyaraj, Chairperson for Academic Affairs of STT44 - Report by Associate Professor Dr.Saiwarun Chaiwanichsiri, Chairperson of STT44 - Her Royal Highness Princess Maha Chakri Sirindhorn graciously presents plaques to Plenary Speaker, 2018 Senior Scientist, 2018 Thailand Outstanding Scientist, 2018 Outstanding Technologist, 2018 Young Scientists, 2018 Young Technologist, 2018 Outstanding Science Teachers, Winners of 2017 National Science Projects Competition, and STT44 Premium Sponsors - Grand Opening Address by Her Royal Highness Princess Maha Chakri Sirindhorn 9:30 Honorable Keynote Lecture by Her Royal Highness Princess Maha Chakri Sirindhorn 10:10 - Associate Professor Dr.Napavarn Noparatnaraporn presents a gift as a token of appreciation to Her Royal Highness Process Maha Chakri Sirindhorn - Brief introduction of Plenary Speakers Dr. Huigen Yang, Former Chair of the Asian Forum for Polar Sciences, Director General of Polar Research Institute of China, People Republic of China and Dr. Branimir Jovanovic, Director of Nikola Tesla Museum, Serbia by Professor Dr. Pairash Tajchayapong, Secretary General of The Information Technology Foundation under the Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn - Plenary Lecture: Overview of Polar Research in the Asian Region by Dr. Huigen Yang - Plenary Lecture: Nikola Tesla and the Problem of Redirecting Human Energy by Dr. Branimir Jovanovic 11:00 - Brief introduction of Professor Dr.Orawon Chailapakul, 2018 Thailand Outstanding Scientist by Associate Professor Dr.Saiwarun Chaiwanichsiri, Chairperson of STT44. Plenary Lecture: Novelty in analytical chemistry by Professor Dr.Orawon Chailapakul 11:15 Her Royal Highness Princess Maha Chakri Sirindhorn visits Commemorative Exhibition and Students Science Projects 12:00 Her Royal Highness Princess Maha Chakri Sirindhorn presides at photo sessions with - Honorable guests - Administrative Committee of the Science Society of Thailand under the Patronage of His Majesty the King - The Council of Science Deans of Thailand Her Royal Highness Princess Maha Chakri Sirindhorn presides at Grand Lunch 13:15 Her Royal Highness Princess Maha Chakri Sirindhorn departs from BITEC 11 The 44 th Congress on Science and Technology of Thailand (STT 44)

18 HONORABLE KEYNOTE SPEAKER Her Royal Highness Princess Maha Chakri Sirindhorn THE 25 th YEAR OF HRH VISIT TO ANTARCTICA Her Royal Highness Princess Maha Chakri Sirindhorn is the first Thai to visit the Antarctica. On a visit in November 1993, Her Royal Highness stayed at Scott Base, the research station operated by the government of New Zealand. Her Royal Highness also visited the USA s McMurdo Station. She learned a great deal about the research at both stations, particularly pertaining to biology, ecology, meteorology and the environment. Following the visit, Her Royal Highness wrote the book Antarctica: Cool Summer, which was a record of her visit to New Zealand and Antarctica. Her Royal Highness wrote that it was my great adventure. On a visit to China in April 2013, Her Royal Highness visited the State Oceanic Administration in Beijing and the Polar Research Institute of China in Shanghai, and received honorary certificates from each of them. Her Royal Highness had communicated, via video conferencing, with Chinese researchers who were working at Chinese Great Wall Station, located on King George Island, Antarctica. She also observed the Icebreaker Xue Long ( 雪龍 -snow dragon) which had just travelled back from the South Pole to Shanghai. The visit was a starting point of the collaboration in the polar research between Thailand and China. An agreement was signed with the Chinese Arctic and Antarctic Administration (CAA) on July 30, NSTDA then coordinated with Thai universities to select Thai researchers whose research are related to the polar science and submitted the list to Her Royal Highness for final selection. Up to this year (2018), there are a total of 13 Thai scientists travelled with Chinese National Antarctic Research Expedition and Japanese Antarctic Research Expedition to do the research at Antarctica. In March 2013 Her Royal Highness Princess Maha Chakri Sirindhorn ventured to the North Pole. While there, she initiated a cooperative project with the government of Norway for Thai scientists to participate in activities at the Norwegian research station in the Svalbard archipelago. During her stay on Svalbard, Her Royal Highness visited several research facilities, including the Svalbard Global Seed Vault, Svalbard Museum and Svalbard Science Centre in Longyearbyen, where she listened to presentations on the research conducted on Svalbard, including research on climate change and biodiversity. She also went on a snowmobile tour of the area. More than half of Svalbard s 61,000 square kilometers is permanently covered by ice and snow. The sun does not rise at all for four months in winter and it does not set in summer. Upon her return, Her Royal Highness asked the Ministry of Foreign Affairs and NSTDA to explore the possibility to develop collaboration with the University Centre in Svalbard (UNIS) and Norwegian Polar Institute (NPI). The MOU on Polar Science between Chulalongkorn University and UNIS has been signed on 13 November 2015, which is the starting point of Arctic research of Thailand. 12 Science and Technology in the Disruptive Era

19 PLENARY SPEAKER Yang Huigen Director General of Polar Research Institute of China OVERVIEW OF POLAR RESEARCH IN THE ASIAN REGION Education B. S. Radio Physics, Wuhan University, China, July, M. S. Radio Physics, Wuhan University, China, July, Ph.D. Space Physics, Wuhan University, China, Oct, Visiting Scholar dispatched by the Ministry of Education from October 1996 October 1997 to study the physics of night-side aurora at Kyoto University of Japan. - JSPS Post-Doctorate Fellow to study the physics of dayside aurora at the National Institute of Polar Research (NIPR) of Japan from March 1998 March Professional Experience Dr. Yang has involved in space physics and carried out wintering aurora observations at Syowa Station in the Antarctic. He developed a conjugate observation of dayside aurora at the Zhongshan Station in the Antarctic and the Yellow River Station on Svalbard in the Arctic. Dr. Yang has been project leader for those capacity-building, including the establishment of the Kulun Station on the top of Dome A, the Taishan Station inland in the Antarctic, the China-Iceland Arctic Science Observatory(CIAO)in the Arctic and building the Xuelong 2 Research Vessel, the first icebreaker made in China. During the International Polar Year (IPY), Dr. Yang served as the chief scientist of the IPY China Program from 2007 to Dr. Yang has intensively involved in Arctic and Antarctic research expeditions. He participated in the 34th Japanese Antarctic Research Expedition (JARE-34, from November 1992 March 1994). He served as the Chief Scientist of the 22nd Chinese National Antarctic Research Expeditions (CHINARE-22 from November 2005 March 2006), the Leader and Chief Scientist of the 25th Chinese National Antarctic Research Expeditions (CHINARE 25 from October 2008 April 2009), and the Leader and Chief Scientist of the 34th Chinese National Antarctic Research Expeditions (CHINARE 34 from November 2008 April 2009). Dr. Yang was the first leader of the Chinese Yellow River Station on Svalbard in the Arctic from July 2005 July He served as the Leader of the 5th Chinese National Arctic Research Expedition, when R/V Xuelong crossed the North-East Passage for the first time and as the first Chinese ship. Dr. Yang developed international research cooperation, and he has been to be the national delegate to the Science Committee of Antarctic Research (SCAR), national delegate and vice president to the International Arctic Science Committee (IASC). He has been a member of the China Committee of the International Council for Science (ICSU) and a steering committee member of the Shanghai Association of Science and Technology. He is a council member of the Chinese Society of Space Science and a council member of the Chinese Society of Geophysics. 13 The 44 th Congress on Science and Technology of Thailand (STT 44)

20 PLENARY SPEAKER Branimir Jovanovic Nikola Tesla Museum, Belgrade, Serbia NIKOLA TESLA AND THE PROBLEM OF REDIRECTING HUMAN ENERGY Nikola Tesla was an ingenious scientist, engineer, inventor and founder of natural principles and laws who entrusted world with some very important discoveries and inventions. He was born in 1856 on the border of the Austrian Empire, of Serbian parents most of his life he spent in America, where he created numerous inventions. He had 310 registered patents mostly in electrical engineering. He died in New York in 1943, and his precious heritage which consist of more tha documents and artefacts are today preserved at the Nikola Tesla Museum in Belgrade. The importance of Tesla s most important inventions lie in their potential to create ogroundwork for mutual connection of people all over the globe. Poly-phase system enabled the use of energy without material transfer and contributed to the development of modern industrial civilization. System of four-circuits in resonance his key patent in the field of wireless communications became foundation of modern radio technology and wireless signal broadcast. After the inventions of resonant frequency of the Earth in 1899 used as groundwork for his project world wireless problem of power he became aware of the revolution in all aspects of human life that his and similar inventions would bring. In 1900, he wrote and published a study called Problem of Increasing Human Energy in the United States, where he presented some of the problems that mankind would encounter in the near future and he came up with certain solutions. Project had been rejected by the leading financiers in America who did not want to invest money in his project which appeared too ambitious and unlikely to be carried out. Deeply disapointed Tesla took a more critical stand regarding the developmental tendencies of modern world and set out predictions about the consequences related to certain trends. It was in the 1920s and 1930s when he spoke of the need to preserve mankind energy supplies, to seek for new, renewable and alternative energy resources, to look for more moderate development and lesser consumption, to strive for the culture oriented to human values, genuine education, etc. Tesla s value as authentic and early thinker of globalism is unknown to the wider public, even unrevealed and unrecognized among experts. Branimir Jovanovic received his B.A. in history and philosophy of science from the University of Zagreb, Croatia in He received his Ph.D. in mechanical engineering from the University of Belgrade, Serbia. 14 Science and Technology in the Disruptive Era

21 PLENARY SPEAKER Orawon Chailapakul Electrochemistry and Optical Spectroscopy Center of Excellence (EOSCE), Department of Chemistry, Faculty of Science, Chulalongkorn University NOVELTY IN ANALYTICAL CHEMISTRY Our laboratory has focused on the development of a new sensor materials for electrochemical and colorimetric detections. Over the past year decades, the boron-doped diamond (BDD) for the analysis of sulfur containing compounds was first conducted by our group. According to our discovery, the use of BDD has been explored in many applications such as antibiotic, β-agonists, dyes, DNA, proteins, iodides, sulfites, and heavy metals. Later, the novel detection platform called paper-based analytical device (PAD) was pioneered in Thailand by our laboratory. PADs were successfully utilized as both electrochemical and colorimetric sensors to create low cost, portability, and disposability platforms. These PAD sensors were tested to different types of analytes such as heavy metals, glucose, cholesterol, uric acid, as well as acetylcholinesterase. Moreover, the dual detection (electrochemical and colorimetric detections) using PADs were developed for quantification and simultaneous detection of heavy metals including lead, cadmium, copper, nickel, iron, and chromium. Finally, pathogen and biomarkers of severe disease are considered with applications for targeting routine health checks and point-of-care testing (POCT). Based on PADs platforms, the colorimetric assay by aggregation of nanoparticles towards target analytes is reported as an alternative colorimetric approach. Meanwhile, the biomarker such as serotonin, neurotransmitter, C-reactive protein (CRP), and human papillomavirus (HPV) were investigated by electrochemical PADs sensors with high sensitivity and high selectivity without any interferences. The novel findings have opened doors for portable analytical tool that are suitable for field analysis and possibility of new automatic platforms for screening and determination of various predictors of health status, disease risk, food quality, and environmental safety. These approaches might be the alternative sensing devices for a health care testing of end users in near future. Professor Dr. Orawon Chailapakul received her Ph.D. in Analytical Chemistry from Department of Chemistry, The university of New Mexico, USA in She initiated BDD electrode and lab-on-paper research in Thailand. Her honors and awards include a TRF Senior Research Scholar Award from the Thailand Research Fund in 2014, a CST Distinguished Chemist Award from Chemical Society of Thailand in 2016, National Distinguished Researcher Award in 2013, and the 2018 Outstanding Scientist. 15 The 44 th Congress on Science and Technology of Thailand (STT 44)

22 PROGRAM FOR SPECIAL SESSIONS, MEETINGS, WORKSHOPS, INVITED LECTURES AND ORAL PRESENTATIONS 29 th October 2018 SPECIAL SESSION (SP1: POLAR RESEARCH) ROOM: GH202 Time Title Speaker 14:00-16:00 POLAR RESEARCH Dr. Huigen Yang Former Chair of the Asian Forum for Polar Sciences, Director General of Polar Research Institute of China, People Republic of China Dr. Satoshi Imura Vice Director-General National Institute of Polar Research, Japan Dr. Hyoung Chul Shin Korea Polar Research Institute Prof.Dr. Kim Holmén International director Norwegian Polar Institute Assoc.Prof.Dr. Voranop Viyakarn Department of Marine Science Chulalongkorn University Assoc.Prof.Dr. Suchana Chavanich (Moderator) SPECIAL SESSION (SP3: INNOVATIVE IMMUNOTHERAPHY OF HIV/AIDS AND CANCER WITH EDIBLE PLANT EXTRACTS) ROOM: GH203 Time Title Speaker 15:00-17:00 INNOVATIVE IMMUNOTHERAPHY OF HIV/AIDS Prof.Dr. Pichaet Wiriyachitra AND CANCER WITH EDIBLE PLANT EXTRACTS Asian Phytoceuticals Public Company Limited 16 Science and Technology in the Disruptive Era

23 AASSA WORKSHOP ROOM: MR224 Time 13:30-14:00 14:00-14:20 14:20-14:50 14:50-15:20 15:20-15:30 15:30-17:00 Title/ Speaker Registration Opening of AASSA International Workshop on Promoting role of scientists and media in science and health communication: From policy to practice Welcome address by The President of the Science Society of Thailand under Royal Patronage The President of the National Science Museum, Thailand AASSA s President Dr. Visudtibhan s Presentation and Discussion Deputy Dean for Corporate Communication, Faculty of Medicine Ramathibodi Hospital, Mahidol University Dr. Veerakumanasivam s Presentation and Discussion Director of Young Scientist Network Academy of Science Malaysia (ASM-YSM) (To be confirmed) Coffee Breaks (may be omitted) Country Reports Session 1 AASSA's Contribution to the SDG Goals and the Challenges By Prof. Dr. Kharirul Anuar Bin Abdullah, Malaysia Alternative Medicine Better Cure: Myth or Reality, Role of Communication By Prof. Dr. Zabta Khan Shinwari, Pakistan Role of Scientists in Risk Communication for Enhancing Science Communication By Dr.Dong Chung Shin, Korea DEANS MEETING ROOM: MR222/MR223 Time Title 14:00-16:30 MEETING OF THE COUNCIL OF SCIENCE DEAN OF THAILAND STT45 MEETING ROOM: MR220 Time 15:00-17:00 MEETING ON PREPARATION OF STT45 Title 17 The 44 th Congress on Science and Technology of Thailand (STT 44)

24 SPECIAL SESSIONS, MEETINGS, WORKSHOPS, INVITED LECTURES AND ORAL PRESENTATIONS 30 th October 2018 SPECIAL SESSION (SP1: THAILAND ANTARCTIC-ARCTIC EXPEDITIONS) ROOM: GH202 Time Title Speaker 9:00-10:30 THAI ANTARCTIC-ARCTIC EXPEDITIONS Assoc. Prof. Dr. Voranop Viyakarn Department of Marine Science, Faculty of Science, Chulalongkorn University Assoc. Prof. Dr. Suchana Chavanich Department of Marine Science, Faculty of Science, Chulalongkorn University Assoc. Prof. Dr. Onruthai Pinyakong Department of Microbiology, Faculty of Science, Chulalongkorn University Asst. Prof. Dr. Anukul Buranapratheprat Department of Aquatic Science, Faculty of Science Burapha University Dr. Prayath Nantasin Department of Earth Sciences, Faculty of Science Kasetsart University Dr. Pataporn Kuanui Department of Marine Science, Faculty of Science, Chulalongkorn University (Present address: True Corporation) Assoc. Prof. Dr. Pitsanupong Kanjanapayont Department of Geology, Faculty of Science, Chulalongkorn University Assoc. Prof. Dr. Thasinee Charoentitirat Department of Geology, Faculty of Science, Chulalongkorn University 18 Science and Technology in the Disruptive Era

25 Asst.Prof.Lt.Col.Dr.Kittiphop Promdee Department of Environmental Science, Academic Division, Chulachomklao Royal Military Academy Dr. Teetat Charoenkalunyuta Department of Survey Engineering, Faculty of Engineering, Chulalongkorn University Prof. Dr.David Ruffolo Department of Physics, Faculty of Science, Mahidol University Dr.Alejandro Sáiz Mahidol University Dr. Waraporn Nuntiyakul Department of Physics and Materials Science, Faculty of Science, Chiang Mai University. 19 The 44 th Congress on Science and Technology of Thailand (STT 44)

26 SPECIAL SESSION (SP4: CRYSTALLOGRAPHY) ROOM: MR211 Chairperson: Co-Chairperson: Professor Dr.Nongnuj Muangsin Assistant Professor Dr.Kittipong Chainok Time ID Title Speaker 9:00-9:30 SP4_INV001 CRYSTAL STRUCTURE ENGINEERING OF Hidehiro Uekusa PHARMACEUTICAL CRYSTAL 9:30-10:00 SP4_INV002 CHIRAL SEGREGATION OF SPACE BY Lawrence W-Y. Wong ANIONIC ASSEMBLIES FOUND IN TARTRAMIDE-BASED SPIROBORATE SALTS 10:00-10:15 SP4_005_OA GAS ADSORPTION IN LANTHANIDE Suwadee Jiajaroen METAL-ORGANIC FRAMEWORKS BASED ON TETRABROMOTEREPHTHALIC ACID 10:15-10:30 SP4_013_OA TWO NOVEL CADMIUM(II) COORDINATION POLYMERS : SYNTHESIS, CHARACTERIZATION, CRYSTAL STRUCTURES AND PHOTOCATALYSIS Ploy Assavajamroon Chairperson: Co-Chairperson: Assistant Professor Dr.Nanthawat Wannarit Dr. Thapong Theerawattananond 10:50-11:20 SP4_INV003 KRYPTORACEMATES! Edward R. T. Tiekink 11:20-11:40 SP4_INV004 COMPUTATIONAL STRUCTURE Sareeya Bureekaew PREDICTION OF (4,4)-CONNECTED COPPER PADDLE-WHEEL-BASED MOFS 11:40-12:00 SP4_007_OA S UPRAMOLECULAR AND HIRSHFELD SURFACE ANALYSES IN SCHIFF BASE COBALT(II) COMPLEXES Siripak Jittirattanakun Chairperson: Co-Chairperson: Assistant Professor Dr.Puey Ounjai Dr. Penchit Chitnumsub 13:00-13:40 SP4_INV005 BETA-GLUCOSIDASE CRYSTAL STRUCTURES FROM PLANTS AND BACTERIA AND THEIR USE IN MODELING HUMAN DISEASE 13:40-14:10 SP4_INV006 SYNCHROTRON RADIATION X-RAY MICROTOMOGRAPHY: FROM CLINIC TO RESEARCH APPLICATIONS 14:10-14:30 SP4_009_OA X-RAY CRYSTALLOGRAPHY of α- GLUCOSIDASE FROM WEISSELLA CONFUSA BBK-1 James R. Ketudat-Cairns Catleya Rojviriya Karan Wangpaiboon Chairperson: Assistant Professor Dr. Supakorn Boonyuen Time ID Title Speaker 14:50-15:30 SP4_INV007 CHEMICAL CRYSTALLOGRAPHY Prathapa Jagannatha 15:30-16:00 FLASH PRESENTATIONS 20 Science and Technology in the Disruptive Era

27 SPECIAL SESSION (SP5: zspace 3D VIRTUAL REALITY TECHNOLOGY FOR STEM LEARNING LAB) ROOM: MR213 Time Topic/Speaker 14:00-14:30 Background of zspace by Mr. Nat Chalanukraw, Strategic Business Development Manager, WORATHAN Technology Co., Ltd. 14:30-16:00 How to apply zspace to STEM Education Development in the classroom by Assistant Professor Dr. Pantip Timsooksai 21 The 44 th Congress on Science and Technology of Thailand (STT 44)

28 SESSION A: AGRICULTURAL SCIENCE / BIOTECHNOLOGY ROOM: MR222 Chairperson: Associate Professor Dr.Aphichart Karnchanatat Time ID Title Speaker 9:00-9:30 A_INV001 SUCCINIC ACID PRODUCTION FROM Kaemwich Jantama VARIOUS RENEWABLE BIOMASS BY METABOLICALLY ENGINEERED Escherichia coli 9:30-9:45 A_002_OF CONSTRUCTION OF GENETIC LINKAGE Todsapol Kornsri MAP BASED ON SIMPLE SEQUENCE REPEAT MARKERS AND IDENTIFICATION QTL CONTROLLING ECONOMICALLY IMPORTANT TRAITS IN CASSAVA (MANIHOT ESCULENTA CRANTZ) 9:45-10:00 A_023_OF EFFECT OF CALCIUM FROM DIFFERENT Naphatsanan Somngam SOURCES ON CASSAVA GROWN IN WARIN SOIL SERIES 10:00-10:15 A_016_OA ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF ENDOPHYTIC FUNGI Chutima Tanapichatsakul ISOLATED FROM Polyscias fruticosa AGAINST SOUTHERN BLIGHT 10:15-10:30 A_024_OF RESPONSE OF CASSAVA TO DIFFERENT RATE AND TYPE OF MAGNESIUM SOURCES IN SATUK SOILS SERIES Apitsara Charoenphon Chairperson: Associate Professor Dr.Aphichart Karnchanatat 10:50-11:05 A_025_OF EFFECT OF RISK HUSK ASH AND NITROGEN ON GROWTH AND YIELD OF CASSAVA, HUAY BOMG 80 VARIETY, IN YASOTHON SOIL SERIES 11:05-11:20 A_017_OA ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF ENDOPHYTIC FUNGI ISOLATED FROM Litsea Petiolata AGAINST Pestalotiopsis samarangensis 11:20-11:35 A_026_OF RESPONSE OF CASSAVA GROWN IN A LOAMY SAND SOIL TO BENTONITE AND CHEMICAL FERTILIZER 11:35-11:50 A_028_OF RESPONSE OF CASSAVA, KU50 AND HUAY BONG 80 VARIETIES, GROWN IN SATUK SOIL SERIES TO CALCIUM AND NITROGEN Pinate Ruenchan Sarunpron Khreungsai Saovanee Boonrod Teeradon Kardudom 22 Science and Technology in the Disruptive Era

29 Chairperson: Associate Professor Dr.Aphichart Karnchanatat Time ID Title Speaker 13:00-13:30 A_INV002 A NOVEL TYPE BETA-GLUCAN Wai Prathumpai PRODUCTION BY Ophiocordyceps dipterigena BCC 2073 FOR INDUSTRIAL APPLICATIONS 13:30-13:45 A_015_OF SMART SYSTEM FOR NFT HYDROPONICS FARM Suppakarn Chansareewittaya 13:45-14:00 A_019_OA GENOME-WIDE ANALYSIS OF THE DOF Gholamreza Khaksar GENE FAMILY IN DURIAN REVEALS RIPENING-ASSOCIATED AND CULTIVAR- DEPENDENT DOF TRANSCRIPTION FACTORS 14:00-14:15 A_021_OF SULFUR AVAILABILITY IN A TYPIC Nuttharuja Hirunburana PALEUSULT AMEDED WITH DIFFERENT SULFUR RICH SOIL CONDITIONERS 14:15-14:30 A_027_OF EFFECT OF RICE HUSK ASH ON VIRING CANE GROWN IN LOAMY SAND SATUK SOIL SERIES Souksady Insixiengmay Chairperson: Associate Professor Dr.Aphichart Karnchanatat 14:50-15:05 A_029_OF NITROGEN UTILIZATION EFFICIENCY AND METABOLISM OF DAIRY GOATS FED POMEGRANATE SEED PULP AND SOYBEAN OIL 15:05-15:20 A_031_OA EFFECT OF CONVERSION OF PADDY FIELD INTO OIL PALM CULTIVATION ON SOIL ORGANIC CARBON 15:20-15:45 A_022_OF EFFECT OF CASSAVA TAILS AND STALK AND POTASSIUM ON CASSAVA, HUAY BONG 80 VARIETY, GROWN IN WARIN SOIL SERIES Jongkolnee Yaowapaksophon Prachwanee Pibumrung Thanaphon Jenwitheesuk 23 The 44 th Congress on Science and Technology of Thailand (STT 44)

30 SESSION B: BIOLOGICAL SCIENCE ROOM: MR212 Chairperson: Co-Chairperson: Associate Professor Dr.Tuangporn Suthiphongchai Associate Professor Dr.Chanitra Thuwajit, MD Time ID Title Speaker 9:00-9:30 B1_INV001 PERSONALIZED NEOANTIGEN-BASED Trairak Pisitkun CANCER IMMUNOTHERAPY 9:30-10:00 B4_INV001 USING PERIPHERAL BLOOD FOR CANCER Prapat Suriyaphol SCREENING BY HIGH-GRADIENT MAGNETIC SEPARATION AND MACHINE LEARNING : PROSTATE CANCER AS A MODEL 10:00-10:30 B4_INV002 TOWARDS A DATA-DRIVEN COMPUTATIONAL METHOD FOR GPCRS DRUG DISCOVERY Sarana Nutanong Chairperson: Co-Chairperson: Associate Professor Dr.Tuangporn Suthiphongchai Associate Professor Dr.Chanitra Thuwajit, MD 10:45-11:15 B1_INV002 THE IMPACT OF MICROBIOTA ON YOUR HEALTH 11:15-11:45 B1_INV003 THE PHAGE NUCLEUS; ITS ROLE FOR PHAGE REPRODUCTION AND ITS IMPLICATION TO EUKARYOTIC CELL NUCLEUS 11:45-12:05 B1_010_OF EXOPOLYSACCHARIDE PRODUCED BY Bacillus subtilis Ly 7/16 ISOLATED FROM THAI TRADITIONAL FERMENTED SOYBEAN AND ITS BIOACTIVITIES Stitaya Sirisinha Vorrapon Chaikeeratisak Vongsathorn Ngampuak Chairperson: Co-Chairperson: Associate Professor Dr.Chanitra Thuwajit, MD Associate Professor Dr.Tuangporn Suthiphongchai 13:00-13:30 B1_INV004 ROLE OF THE UBIQUITIN-LIKE PROTEIN HUB1 IN PRE-MRNA SPLICING CONTROL 13:30-14:00 B1_INV005 ON-SITE LAMP-BASED DETECTION PLATFORMS FOR MEDICAL DIAGNOSTICS 14:00-14:20 B1_001_OA KT2 AND RT2 ANTIMICROBIAL PEPTIDES MODIFIED FROM SIAMESE CROCODILE LEUCROCIN I, PROMOTES ANTICANCER ACTIVITY IN HUMAN COLON CANCER HCT- 116 CELLS Sittinan Chanarat Wansika Kiatpathomchai Surachai Maijaroen 24 Science and Technology in the Disruptive Era

31 Chairperson: Co-Chairperson: Associate Professor Dr.Chanitra Thuwajit, MD Associate Professor Dr.Tuangporn Suthiphongchai Time ID Title Speaker 14:50-15:20 B4_INV003 METABOLOMICS AND SYSTEMS BIOLOGY Sakda Khoomrung FOR THE DEVELOPMENT OF HUMAN VACCINE 15:20-15:40 B1_002_OF EFFECT of Morinda citrifolia L. ROOT ETHANOLIC EXTRACT ON SK-N-SH NEUROBLASTOMA CELLS INDUCED BY Kamonwan Srisawad H2O2 15:40-16:00 B4_001_OF SUPPRESSION OF AFLATOXIN B1- INDUCED INTRACELLULAR REACTIVE OXYGEN SPECIES PRODUCTION AND CYTOTOXICITY IN HEPG2 CELLS BY PURPLE WAXY CORN EXTRACTS Tichakorn Singto 25 The 44 th Congress on Science and Technology of Thailand (STT 44)

32 SESSION C: CHEMISTRY ROOM: MR223 Chairperson: Co-Chairperson: Associate Professor Dr.Panote Thavarungkul Dr. Prasat Kittakoop Time ID Title Speaker 9:00-9:30 C3_INV001 QUINOLINE-BASED STYRYL DYES FOR Tirayut Vilaivan DUAL-MODE COLORIMETRIC AND FLUORESCENCE SENSING OF DNA 9:30-10:00 C1_INV001 SECOND GENERATIONS OF DISPERSIVE Supalax Srijaranai LIQUID LIQUID MICROEXTRACTION 10:00-10:20 C1_010_OA DEVELOPMENT OF MICROFLUIDIC Suphakorn Katib DEVICES FOR DETERMINATION OF TOTAL CHOLESTEROL IN BLOOD 10:20-10:40 C3_003_OA DIRECT C-H OXIDATION OF 2,4-DIAMINO- 6-TRIFLUOROMETHYLPYRIMIDINE WITH PHTHALOYL PEROXIDE Natthakorn Uppatam Chairperson: Co-Chairperson: Professor Dr.Thawatchai Tuntulani Assistant Professor Dr.Nawee Kungwan 10:50-11:20 C4_INV001 EFFECTS OF DIFFERENT PROTON DONOR AND ACCEPTOR GROUPS ON EXCITED- STATE INTRAMOLECULAR PROTON TRANSFERS OF AMINO-TYPE AND HYDROXY-TYPE HYDROGEN BONDING MOLECULES: THEORETICAL INSIGHTS 11:20-11:50 C2_INV001 STRUCTURE-FUNCTION RELATIONSHIPS OF TRANSITION METAL COORDINATION POLYMERS 11:50-12:10 C2_001_OA SOLID PREMIX FOR WATER DECONTAMINATION CONSISTING OF SOLID HYDROGENPEROXIDE AND ZEROVALENT IRON Nawee Kungwan Jaursup Boonmak Christoph Sontag Chairperson: Co-Chairperson: Professor Dr.Tirayuth Vilaivan Dr.Chularat Wattanakit Time ID Title Speaker 13:00-13:30 C1_INV002 ELECTROCHEMICAL CHEMICAL SENSORS Panote Thavarungkul AND BIOSENSORS BASED ON POROUS STRUCTURE MATERIALS 13:30-14:00 C3_INV002 PERSPECTIVE ON NATURAL PRODUCTS Prasat Kittakoop CHEMISTRY AND GREEN CHEMISTRY 14:00-14:20 C1_007_OA DEVELOPMENT OF COLORIMETRIC Lalida Chanchaem METHOD FOR SCREENING OF ALPHA- GLUCOSIDASE INHIBITORS FROM PLANT EXTRACT 14:20-14:40 C3_001_OA CHEMICAL CONSTITUENTS FROM TWIGS AND LEAVES OF Goniothalamus cheliensis Wuttichai Jaidee 26 Science and Technology in the Disruptive Era

33 Chairperson: Co-Chairperson: Assistant Professor Dr.Jaursup Boonmak Dr. M. Paul Gleeson Time ID Title Speaker 14:40-15:20 C4_INV002 APPLICATION OF SYNTHETIC AND M. Paul Gleeson COMPUTATIONAL CHEMISTRY METHODS IN ANTI-MALARIAL DRUG DISCOVERY 15:20-15:50 C2_INV002 ENANTIOSELECTIVE SYNTHESIS AND Chularat Wattanakit SEPARATION AT MESOPOROUS CHIRAL METAL SURFACES 15:50-16:10 C2_002_OA PREPARATION OF ZINC-ALUMINUM LAYERED DOUBLE HYDROXIDES FOR Cholaphan Deeleepojananan PHOSPHATE ADSORPTION USING MICROWAVE-ASSISTED PRECIPITATION METHOD 16:10-16:30 C4_004_OA STUDY OF THE SYMMETRICAL PINCER Siriwat Shiraarnon PALLADACYCLE FORMATION REACTION THROUGH DILIGATION: DFT CALCULATION 16:30-16:50 C3_002_OA CHEMICAL CONSTITUENTS FROM THE Pornphimol Meesakul LEAF AND TWIG EXTRACTS OF Desmos cochinchinensis 16:50-17:10 C4_007_OA PENETRATION OF ALPHA-MANGOSTIN INTO THE POPC MEMBRANE: A MOLECULAR DYNAMICS SIMULATION STUDY Wiparat Hotarat 27 The 44 th Congress on Science and Technology of Thailand (STT 44)

34 SESSION F: PHYSICS / APPLIED PHYSICS ROOM: MR221 Chairperson: Co-Chairperson: Professor Dr.Pisith Singjai Associate Professor Dr.Udomsilp Pinsuk Time ID Title Speaker 13:00-13:30 F_INV001 SCIENCE UNDER HIGH PRESSURE: THE Thiti Bovornrattanarat NEW ALCHEMISTS 13:30-13:50 F_001_OA EXPERIMENTAL-BASED PROBABILITY Pawaphat Jaturaphagorn INTERPRETATION OF PHOTON-RELATED EVENTS IN DIRAC THREE-POLARIZER EXPERIMENTS 13:50-14:10 F_006_OF EFFECT OF ANNEALING TEMPERATURE Intira Nualkham ON THERMOELECTRIC PROPERTIES OF GERMANIUM ANTIMONY TELLURIDE THIN FILMS PREPARED BY PULSED DC MAGNETRON SPUTTERING 14:10-14:30 F_008_OA RADIOLOGICAL HAZARD ASESSMENT AND EXCESS LIFETIME CANCER RISK EVALUATION IN SURFACE SOIL SAMPLES COLLECTED FROM KLAENG DISTRICT IN RAYONG PROVINCE, THAILAND Prasong Kessaratikoon Chairperson: Co-Chairperson: Professor Dr.Pisith Singjai Associate Professor Dr.Udomsilp Pinsuk 14:50-15:10 F_007_OF DENSITY FUNCTIONAL THEORY STUDY OF STRAIN-INDUCED BAND GAP TUNABILITY OF TWO-DIMENSIONAL LAYERED MoS2, MoO2, WS2 AND WO2 Rutchapon Hunkao 28 Science and Technology in the Disruptive Era

35 SESSION G: MATHEMATICS / STATISTICS / COMPUTER SCIENCE ROOM: MR220 Chairperson: Co-Chairperson: Associate Professor Dr. Ponrudee Netisopakul Dr. Sirithip Wasinrat Time ID Title Speaker 13:00-13:30 G_INV001 ARE WE READY FOR THE AGING SOCIETY? Nipon Parinyavuttichai A CASE STUDY OF CHA-CHOENG-SAO TOWN MUNICIPALITY 13:30-14:00 G_INV002 TOWARDS OPEN INNOVATION WITH OPEN Marut Buranarach DATA SERVICE PLATFORM 14:00-14:30 G_INV003 3-D MRI BRAIN SCAN CLASSIFICATION: EPILEPSY VERSUS NON-EPILEPSY Akadej Udomchaiporn Chairperson: Co-Chairperson: Assistant Professor Dr. Wichai Witayakiattilerd Dr. Nattapong Kaewboonma 14:50-15:20 G_INV005 AN INCREMENTAL MINING ALGORITHM FOR SHARE FREQUENT ITEMSETS 15:20-15:40 G_002_OF DEVELOPMENT OF APPLICATION FOR TRAFFIC SIGN DETECTION IN PANORAMIC IMAGES USING DEEP LEARNING TECHNIQUE 15:40-16:10 G_INV004 UNDERSTANDING THE LINK BETWEEN CLIMATE CHANGE AND EXTREME WEATHER EVENTS Chayanan Nawapornanan Sathit Prasomphan Chalump Oonariya 29 The 44 th Congress on Science and Technology of Thailand (STT 44)

36 SESSION H: FOOD SCIENCE & TECHNOLOGY ROOM: MR213 Chairperson: Co-Chairperson: Associate Professor Dr. Kanitha Tananuwong Dr. Somruedee Thaiphanit Time ID Title Speaker 9:00-9:40 H_INV001 DESIGNING FOODS FOR THE SILVER Anadi Nitithamyong GENERATION: AN INNOVATION CHALLENGE 9:40-10:20 H_INV002 BIOFUNCTIONAL AND PHYSICOCHEMICAL Parichat Hongsprabhas PROPERTIES OF RECONSTITUTED OKRA MUCILATE-MALTODEXTRIN SPRAY-DRIED POWDER 10:20-10:40 H_005_OF PHYSICOCHEMICAL, PHENOLIC COMPOUNDS AND IN VITRO DIGESTIBILITY OF THAI PURPLE RICE VARIETY INFLUENCED BY GERMINATION CONDITIONS Iyiola Oluwakemi Owolabi Chairperson: Co-Chairperson: Associate Professor Dr. Kanitha Tananuwong Dr. Somruedee Thaiphanit 11:00-11:20 H_014_OF A PROTEASE-RESISTANT α- GALACTOSIDASE FROM PAENIBACILLUS POLYMYXA STRAIN PB5 (WITH GOOD HYDROLYTIC ACTIVITY TOWARDS RAFFINOSE FAMILY OLIGOSACCHARIDES) 11:20-11:40 H_018_OF A COMPARATIVE STUDY OF SENSORY CHARACTERISTICS ON PINEAPPLE GROWN IN CHIANG RAI WITH DIFFERENT CULTIVARS AND CULTIVATION METHODS Sreyneang Nhim Chirat Sirimuangmoon 30 Science and Technology in the Disruptive Era

37 ANNUAL MEETING ROOM: GH203 Time Title 12:00-13:30 ANNUAL MEETING OF THE SCIENCE SOCIETY OF THAILAND UNDER THE PATRONAGE OF HIS MAJESTY THE KING Time 09:00-10:30 10:30-12:00 12:00 13:00 13:00 14:30 14:30 16:00 AASSA WORKSHOP ROOM: MR224 Title/ Speaker Country Reports Session 2 Engagement of Scientific for the Promotion of Modern Biotech Products in Bangaladesh By Prof. Dr. Md. Shahidul Haque, Bangaladesh Communicating Science - Perspective and Challenges By Dr Ranjit Mahindapala, Sri Lanka Promoting role of Scientists in Public Policy Through the Media By Gulzar Karybekova, Kyrgyz Republic Country Reports Session 3 Role of Indian Science Academies in Science Communication to Society By Chandrima Shaha, India Development of Influenza Program in Contributing National Campaign of Public Awareness and Education By Dr. Finarya Legoh, Indonesia A Pilot Project Game design for Nutrition Education to Patients with Chronic Disease in Thailand By Dr.Sirikoy Chutataweesawas, Thailand Lunch Country Reports Session 4 DEWATS and its health impact in Afghanistan By Prof.Rahmatgul Ahmadi, Afghanistan Digital Farming Strategy in Precision Agriculture By Prof.Sakae Shibusawa, Japan Interaction of Science and Mass Media in the Kyrgyz Republic By Azyk A. Orozonova, Kyrgyz Republic Country Reports Session 5 Effective practice in communication of health science and technology in Nepal By Prof. Dr. Anjana Maharjan Singh, Nepal Scientists and media collaborate on cancer prevention in Vietnam By Nguyen Tuong Lan, Vietnam Science Communication to Reach to Rural Community By Dr. Buddhi Ratna Khadge, Napal 16:00 17:00 Recommendation Committee Meeting *14:30 17:00 AASSA Executive Board Meeting (held separately) Venue: Room The 44 th Congress on Science and Technology of Thailand (STT 44)

38 CONFERENCE BANQUET ROOM: GRAND HALL Time 17:30-20:15 CONFERENCE BANQUET Title 32 Science and Technology in the Disruptive Era

39 SPECIAL SESSIONS, INVITED LECTURES AND ORAL PRESENTATIONS 31 st October 2018 SPECIAL SESSION (SP2: DISRUPTIVE ERA) ROOM: GH203 Time Title/Speaker 13:30 15:30 Surviving and Managing Changes in this Disruptive Era: How about Thailand? - Dr. Akkharawit Kanjana-Opas Deputy Secretary General National Science Technology and Innovation Policy Office Chief Executive Officer, Food Innopolis - Dr. Chinawut Chinaprayoon, Vice President, Digital Startup Promotion Institute Digital Economy Promotion Agency - Ms. Lena Ng Chief Investment Officer Amata Corporation Public Company Limited - Mr. Monthian Buntan Member of the National Legislative Assembly United Nations Committee on the Rights of Persons with Disabilities Moderator: Mr. Varin Sachdev, News Anchor, TV &Radio Host 33 The 44 th Congress on Science and Technology of Thailand (STT 44)

40 AASSA WORKSHOP ROOM: MR224 Time Title/ Speaker 09:00-09:30 Adoption of Recommendation and Closing 09:30-12:00 AASSA General Assembly 12:00 13:00 Lunch * :00 Transfer to Ancient City 34 Science and Technology in the Disruptive Era

41 SESSION B: BIOLOGICAL SCIENCE ROOM: MR212 Chairperson: Assistant Professor Dr.Kobchai Pattaragulwanit Time ID Title Speaker 9:30-9:50 B2_008_OA DOUBLE-STRANDED RNA ELEMENTS OF PHYTOPYTHIUM CUCURBITACEARUM Thanyanuch Kriangkripipat 9:50-10:10 B2_007_OF ANTIMICROBIAL ACTIVITY OF CHITOSAN AND ITS EFFECT ON PHYSICAL AND Muhammad Iqbal Perdana MECHANICAL PROPERTIES OF SAGO AND CASSAVA STARCH/CLAY FILMS 10:10-10:30 B2_016_OF ISOLATION OF BIOSURFACTANT PRODUCING BACTERIA FROM THAI TRADITIONAL FERMENTED SOYBEAN FOOD Supitchaya Laothong Chairperson: Co-Chairperson: Associate Professor Dr.Supachitra Chadchawan Assistant Professor Dr.Kobchai Pattaragulwanit 10:50-11:20 B2_011_OF CHARACTERIZATIONS OF CIPROFLOXACIN RESISTANCE IN Klebsiella pneumoniae ISOLATED FROM PATIENTS IN MEDICINE WARD, SONGKLANAGARIND HOSPITAL 11:20-11:40 B3_005_OF CONVOLUTIONAL NEURAL NETWORK AS A TOOL FOR HIGH-THROUGHPUT IDENTIFICATION OF REGULATORY SEQUENCES IN GENOMIC DNA Arnon Chukamnerd Siwat Ruangroengkulrith 35 The 44 th Congress on Science and Technology of Thailand (STT 44)

42 SESSION D: POLYMER & MATERIALS SCIENCE / NANOTECHNOLOGY ROOM: MR211 Chairperson: Co-Chairperson: Professor Dr.Supon Ananta Associate Professor Dr.Taweechai Amonsakchai Time ID Title Speaker 8:00-8:30 D_INV001 FAST DECAY TIME OF CE 3+ DOPED IN SODIUM-GALDOLINIUM-ALUMNIUM- PHOSPHATE FOR SCINTILLATION MATERIAL APPLICATION Jakrapong Kaewkhao 8:30-9:00 D_INV002 MODIFIED CASHEW NUT SHELL LIQUID AS CURING AGENT AND ORGANO-MODIFIER IN LDH FILLED NATURAL RUBBER 9:00-9:20 D_010_OF SYNTHESIS OF CDTE- MERCAPTOSUCCINIC ACID AND IN VITRO STUDY OF ITS CYTOTOXICITY USING MTT AND LDH ASSAYS 9:20-9:40 D_024_OA ETHANOL OXIDATION REACTION ON NICKEL-BASED CATALYSTS 9:40-10:00 D_012_OF PREPARATION OF HYDROXYAPATITE AND PLATINUM-HYDROXYAPATITE FIBERS BY ELECTROSPINNING TECHNIQUE Anoma Thitithammawong Sonam Wangchuk Wilaiwan Supap Titapa Tanawansombat Chairperson: Co-Chairperson: Associate Professor Dr.Naratip Vittayakorn Associate Professor Dr.Jakrapong Kaewkhao 10:20-10:50 D_INV003 GIANT DIELECTRIC CONSTANTS IN LEPIDOCROCITE-TYPE TITANATE WITH INTERLAYER ALKALI IONS AND EDGE- SHARED TiO6 MOTIFS 10:50-11:10 D_001_OF DEVELOPMENT OF BIO-BASED COMPATIBILIZED PLA/NR BLENDS AND DIY MINI-EXTRUDER FOR FABRICATION OF 3D PRINTING FILAMENTS 11:10-11:30 D_015_OA DEVELOPMENT OF ELECTROACTIVE POLYMER ACTUATORS EXPLOITING LOW COST PRECISE OPTOELECTRONIC MIRROR TECHNOLOGY 11:30-11:50 D_009_OF PREPARATION OF NOVEL HIERARCHICALLY MAGNETIC POROUS CARBON MONOLITHS BY SURFACE SELF- ASSEMBLY COATING ON SUGARCANE BAGASSE SCAFFOLD 11:50-12:10 D_027_OA CARBON ADSORBENTS FROM SUGARCANE BAGASSE PREPARED THROUGH HYDROTHERMAL CARBONIZATION FOR ADSORPTION OF METHYLENE BLUE: EFFECT OF HEAT TREATMENT ON ADSORPTION EFFICIENCY Tosapol Maluangnont Pattiya Pibulchinda Kritsadi Thetpraphi Ratchadaporn Kueasook Preeyanit Buapeth 36 Science and Technology in the Disruptive Era

43 SESSION E: ENERGY / ENVIRONMENTAL& EARTH SCIENCE ROOM: MR222 Chairperson: Co-Chairperson: Associate Professor Dr.Kraichat Tantrakarnapa Assistant Professor Dr.Achariya Suriyawong Time ID Title Speaker 9:00-9:30 E_INV001 THE HAZE EPISODE IN THE NORHTERN Kraichat Tantrakarnapa PART OF THAILAND AND THE SYSTEM APPROACH FOR FACING THE SITUATION RIGHT, ALL CAPS) 9:30-9:50 E_002_OF FOUNDATION THE WEIGHT SET FOR THE Bui Viet Hung WATER QUALITY INDEX S FORMULA 9:50-10:10 E_007_OF ASSESSMENT OF COLIFORM BACTERIA CONTAMINATION IN DRINKING WATER Korakot Chansareewittaya SOURCES OF HILL TRIBE LIVING AREA IN CHIANG RAI 10:10-10:30 E_012_OF INDOOR AIR QUALITY ASSESSMENT IN GOVERNMENT HOSPITAL IN NORTHERN THAILAND Sitang Kongkratoke Chairperson: Co-Chairperson: Associate Professor Dr.Kraichat Tantrakarnapa Assistant Professor Dr.Sombat Yumuang 10:50-11:20 E_INV002 SPATIAL LANDSLIDE HAZARD AND RISK ASSESSMENT IN THAILAND 11:20-11:40 E_003_OA SEISMIC GEOMORPHOLOGY OF GIANT FORESETS FORMATION, TARANAKI BASIN, NEW ZEALAND 11:40-12:00 E_004_OF SEISMIC CHARACTERISTICS OF PALEO- POCKMARKS AT THE GREAT SOUTH BASIN, NEW ZEALAND Sombat Yumuang Waris Nuamnim Arunee Karaket Chairperson: Co-Chairperson: Associate Professor Dr.Kraichat Tantrakarnapa Associate Professor Dr.Pitsanupong Kanjanapayont 13:00-13:30 E_008_OF LONG CHAIN N-ALKANE DISTRIBUTIONS IN THE MANGROVE PLANTS 13:30-13:50 E_010_OF OXIDATION STATES OF TRACE ELEMENTS IN SYNTHETIC CORUNDUM 13:50-14:10 E_009_OF APPLICATION OF DIFFERENTIAL SAR INTERFEROMETRY FOR GROUND DISPLACEMENT MONITORING IN WESTERN MOUNTAIN RANGE BESIDE THE PHETCHABUN BASIN, CENTRAL THAILAND Assuma Sainakum Ontima Yamchuti Nuttavit Kumvijairat 37 The 44 th Congress on Science and Technology of Thailand (STT 44)

44 ABSTRACTS FOR SPECIAL SESSIONS AND INVITED LECTURES 38 Science and Technology in the Disruptive Era

45 A_INV001 Kaemwich Jantama School of Biotechnology, Suranaree University of Technology SUCCINIC ACID PRODUCTION FROM VARIOUS RENEWABLE BIOMASS BY METABOLICALLY ENGINEERED Escherichia coli Succinate has been recognized as a potential key platform chemical for several industrial applications including biodegradable plastics and green polymers. It is primarily produced by hydrogenation of maleic anhydride, which is both expensive and environmentally unfriendly. Succinate has been classified as one of the top value-added chemicals that can be produced from biomass by the US Department of Energy (DOE). Therefore, the use of renewable biomass for the succinate production may present an attractive solution to reduce costs and an alternative sustainable process to escape the utilization of fossil sources. Escherichi coli KJ122 was previously developed to produce succinate from glucose under simple anaerobic batch fermentation in mineral salts medium with the highest yield closed to the theoretical maximum ever reported. In our lab, we further developed many derivatives of E. coli KJ122 strain to be able to perform the succinate production platform utilizing various agricultural biomass including sugarcane molasses, sucrose, sugarcane bagasse, cassava pulp and starch, xylose and even rice straw. Our developed fermentation process and strains could demonstrate the efficient succinate production at very impressive titer in the range of g/l at the yield equivalent to that of glucose. It is likely to be a promising platform for an economically feasible succinate production in the industrial scale. Associate Professor Kaemwich Jantama received his B.Sc. in Food Science and Technology from Chiang Mai University, Thailand. He continued pursuing his M.S. in Molecular Genetics and Genetic Engineering at Mahidol University, Thailand. After that he also finished his M.E and Ph.D. in Chemical Engineering at University of Florida, USA.. His research interests involve metabolic engineering and bioprocess engineering for applications in sustainable productions of renewable fuels and biochemicals from biomass. 39 The 44 th Congress on Science and Technology of Thailand (STT 44)

46 A_INV002 Wai Prathumpai Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand A NOVEL TYPE BETA-GLUCAN PRODUCTION BY Ophiocordyceps dipterigena BCC 2073 FOR INDUSTRIAL APPLICATIONS A novel type of _-glucan of Ophiocordyceps dipterigena BCC 2073 has a size of kda which is 20 times bigger than yeast s β-glucan. Its structure composes of a (1, 3)- β- D-glucan backbone, substituted with 0-6 side chains of (1, 3)- β-d-pyranosyl unit. It is biocompatible, non-cytotoxic and a strong inducer of interleukin-8 (IL-8), a cytokine responsible for enhancing wound-healing process. Because of its structure and physical properties, it is possible for prebiotic usage. Its prebiotic properties in vitro and in vivo were studied, leading to new application of this β-glucan. It can be produced in submerged culture fermentation. The production yield of this β-glucan by O. dipterigena BCC 2073 is higher (60 g/l) than other commercial β-glucans that have been reported. This mass production can reduce the cost of production and it can be applied in various applications. With the properties of non-cytotoxic, good gel-forming, highly moisturizing, high viscosity, well-adhesiveness and good tensile strength, this β-glucan can be used in cosmetic, food and feed, medical and pharmaceutical, and petroleum applications to replace high cost β-glucan from other sources. The size of the β-glucan can also be reduced by gamma-radiation and customised for specific application. Dr. Wai Prathumpai received his master degree in Bioprocess Technology from the Asian Institute of Technology and Ph.D. in Process Biotechnology from the Technical University of Denmark. His research interests are in the field of process biotechnology, production of bioactive compounds and other biological molecules, fermentation and bioprocess development. He published more than 25 papers in the Sciencedirect lists and 3 patents. 40 Science and Technology in the Disruptive Era

47 B1_INV001 Trairak Pisitkun Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University PERSONALIZED NEOANTIGEN-BASED CANCER IMMUNOTHERAPY Although advanced cancer therapeutics have already existed in recent years, the high failure and low survival rate are still existent. The primary reason for the disappointment of those treatments is possibly from the high heterogeneity of genetic background in different types of cancer as well as in different individual patients. So, the unmet need for new treatment options that can be tailored for each person is urgently required. Cancer immunotherapy has come to be a robust approach for several types of cancer because it exploits our immune systems to attack cancer cells, so it is a promising strategy to eliminate cancer. To develop personalized cancer immunotherapy such as cancer vaccine, it is essential to characterize the specific targets which can be selectively recognized by the immune system. Cancer cells classically contain a high number of somatic mutations that do not emerge in normal cells. Those mutated peptides can be presented on the tumor cell surface in complex with human leukocyte antigen (HLA) class I protein; this HLA-mutatedpeptide complex could potentially be immunogenic and recognized by T cells as a neoantigen. Therefore, identification of neoantigens is an essential step to improve the development of cancer vaccine approach and help in the prediction of patient response to checkpoint inhibitors. Here we discuss our progress in the development of personalized neoantigenbased cancer immunotherapy. Dr. Pisitkun s biography Education: M.D., Board of Clinical Nephrology, Postdoctoral Fellow at Epithelial Systems Biology Laboratory, National Institutes of Health, Maryland, USA Present position: Principal Investigator, Systems Biology Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand Research interest: Systems Biology, Proteomics, Bioinformatics, Cell signaling Major recognition/awards/achievements: Outstanding Mentorship Award, NHLBI, NIH 41 The 44 th Congress on Science and Technology of Thailand (STT 44)

48 B1_INV002 Stitaya Sirisinha Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand THE IMPACT OF MICROBIOTA ON YOUR HEALTH It is generally accepted that microbiota can profoundly influence a diverse aspect of host physiology, from nutrient and energy metabolism, defense against infection to development, education and tuning of immune system. However, the exact molecular mechanisms of this association remain to be elucidated. Host immune system possesses different effector mechanisms which it uses to maintain homeostatic relationship with the microbiota. Healthy gut microbiota profile (e.g., composition, diversity and richness) and their metabolites depend largely on host diet, lifestyle and environmental factors rather than on the status of host genetics. In addition, recent studies demonstrate a critical role of central nervous system (CNS) in tuning and maintaining this balance. Any situation or condition that tips this balance would result in microbial dysbiosis (imbalance/defect) which may be associated with a number of diseases and this list is getting longer and longer. Diseases known to have been associated with altered gut microbiota include for example obesity and cardiovascular diseases. An emerging area of investigation on a crosstalk among microbiota, gut, immune and CNS has also demonstrated a critical role of gut microbiota in orchestrating a number of neurodevelopmental and neurodegenerative disorders, including for instance autism, mood and dementia. However, with the exception of obesity and some metabolic diseases like diabetes and cardiovascular diseases, it remains unclear if the altered microbiota observed is a causal or consequence of the diseases. A better understanding on this interrelationship will provide us with new knowledge on disease pathogenesis which may lead to novel approaches and strategies, including microbiotabased therapies that may be safer and more effective than the current management. Satitaya Sirisinha, DDS., Ph.D. is an Emeritus Professor in Mahidol University. He graduated Ph.D. from University of Rochestor, USA. He is an expert on immunology and molecular biology of tropical diseases, parasitic diseases, liver fluke infection, cholangiocarcinoma, melioidosis, microbiota and etc. He published more than 170 papers in high impact journals and received several awards such as Outstanding Scientist of Thailand Award in 1988, Mahidol Research Award in Science and Technology in the Disruptive Era

49 B1_INV003 Vorrapon Chaikeeratisak Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand THE PHAGE NUCLEUS; ITS ROLE FOR PHAGE REPRODUCTION AND ITS IMPLICATION TO EUKARYOTIC CELL NUCLEUS The cell nucleus is a vital component and serves a crucial role inside the eukaryotic cell. While the biological functions of this key structure are well elucidated, the evidence of its origin is still elusive. Here, we demonstrated an example to support the viral eukaryogenesis theory and explained that the primitive cell nucleus could originate from a bacterial cell that is infected by a virus, known as a bacteriophage. During the infection by a giant Pseudomonas bacteriophage, which is related to the PhiKZ family, the giant phage assembled a nucleus-like structure that enclosed and separated phage DNA from the cell cytoplasm. This structure also segregated proteins according to function. Proteins involved in DNA replication and transcription localized inside the compartment, whereas proteins involved in translation and nucleotide synthesis localized outside. The biological roles of this nucleus-like structure is similar to the eukaryotic cell nucleus thereby the structure is named the phage nucleus. Later during infection, phage-encoded tubulin spindles harnessed dynamic properties to position the phage nucleus near midcell and transported viral capsids, which assembled on the cytoplasmic membrane, to dock on the surface of the nucleus for DNA packaging. In the presence of the spindle mutant, which interrupts the dynamic property of the phage spindle, the filaments failed to position the nucleus and to transport the viral capsids, resulting in inefficient phage reproduction. When multiple infections from different giant phages occurred, the homologs of the nuclear shell protein were able to co-assemble into various forms including a bilayer shell, suggesting that the structure of the phage nucleus is versatile and is able to develop toward the more complicated structure when undergoing through a driving pressure. These results reveal that the phages have evolved a specialized structure for their reproduction and this possibly leads to the first step of evolution toward the primitive form of the eukaryotic cell nucleus. Dr. Vorrapon Chaikeeratisak is currently working as a principal investigator at Department of Biochemistry, Chulalongkorn University, Thailand. After he received Ph.D. in Biochemistry from Chulalongkorn University in 2012, he continued to pursue his career in higher education at University of California, San Diego. He spent 6 years conducting research in bacteriophage biology and successfully published his work in top tier journals. As a PI back here in Thailand, he founded the Microbe lab which mainly focuses on bacteriophage biology and antimicrobial discovery. 43 The 44 th Congress on Science and Technology of Thailand (STT 44)

50 B1_INV004 Sittinan Chanarat Department of Biochemistry, Faculty of Science, Mahidol University ROLE OF THE UBIQUITIN-LIKE PROTEIN HUB1 IN PRE-mRNA SPLICING CONTROL Accurate pre-mrna splicing is required for correct gene expression and relies on faithful splice site recognition. Here, we show that the ubiquitin-like protein Hub1 binds to the DEAD-box helicase Prp5, a key regulator of early spliceosome assembly, and stimulates its ATPase activity thereby enhancing splicing and relaxing fidelity. High Hub1 levels enhance splicing efficiency but also cause missplicing by tolerating suboptimal splice sites and branchpoint sequences. Notably, Prp5 itself is regulated by a Hub1-dependent negative feedback loop. Since Hub1-mediated splicing activation induces cryptic splicing of Prp5, it also represses Prp5 protein levels and thus curbs excessive missplicing. Furthermore, Hub1 expression was induced upon stresses and thereby improving splicing efficiency. Our findings indicate that Hub1 is a component of stress response system and mediates enhanced yet error-prone splicing, a tightly controlled mechanism by a feedback loop of PRP5 cryptic splicing activation. 44 Science and Technology in the Disruptive Era

51 B1_INV005 Wansika Kiatpathomchai Bioengineering & Sensing Technology Laboratory, National Center for Genetic Engineering & Biotechnology (BIOTEC), Pathum thani 12120, Thailand ON-SITE LAMP-BASED DETECTION PLATFORMS FOR MEDICAL DIAGNOSTICS An accelerated, loop-mediated isothermal amplification (LAMP) assay has been developed for sensitive and specific detection of few copies of target nucleic acids of pathogens. It operates under isothermal conditions by self-recurring strand-displacement DNA synthesis using specially designed primer sets. Here, the rapid assay platforms for onsite detection by combining LAMP and use of a portable turbidimeter, use of lateral flow dipsticks, use of oligonucleotide-labeled nanogold probes, and use of electrochemical biosensor will be presented. Since these LAMP methods do not require sophisticated equipment, they are more applicable for small or on-site laboratories. The time required for these integrated LAMP techniques is approximately min. These methods have been successfully applied for detection of human infectious diseases, e.g., Mycobacterium tuberculosis, malaria (Plasmodium facipalum, Plasmodium vivax), human papillomavirus, Hepatitis B virus and methicillin-resistant Staphylococcus aureus. These integrated LAMP techniques are sensitive, simple, rapid, cost-effective and can be manufactured locally, reducing the need for imported diagnostic kits. They can be applied in routine diagnosis and progressed into a realistic point of care detections for use in treatment and control of infectious diseases for resource-poor endemic areas. Mrs. Wansika Kiatpathomchai received M.Sc degree in Biochemistry from Faculty of Science, Mahidol University in She is currently a principal researcher and head of Bioengineering and sensing technology laboratory at BIOTEC Thailand. Her research interests include DNA diagnostic techniques and biosensor. She aims to develop simple, rapid and sensitive diagnostic techniques to detect various types of shrimp, fish, human and foodborne pathogens. She received UNESCO-L Oreal for Women in Science Fellowship in The 44 th Congress on Science and Technology of Thailand (STT 44)

52 B4_INV001 Prapat Suriyaphol Division of Bioinformatics and Data Management for Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand USING PERIPHERAL BLOOD FOR CANCER SCREENING BY HIGH- GRADIENT MAGNETIC SEPARATION AND MACHINE LEARNING :- PROSTATE CANCER AS A MODEL Detecting cancer early, when it can be cured, is the most consequential challenge of modern medicine. Recently it was discovered that cancers as little as 1mm aggressively sprout tiny blood vessels to accelerate growth, and that the cells shed from these fast-growing vessels into the blood are excellent early cancer biomarkers. To detect these tumor-derived Circulating Endothelial Cells (CECs), we use a unique rarecell detection method, which is capable of removing normal blood cells from the sample and expose all types of tumor-derived, atypical cells including CECs. High-gradient magnetic separation in optimized buffer specifically removes white blood cells and provides optimal recovery of atypical cells, large cells and even cell clumps via flow channels several hundred micrometers wide. We has refined semi-automated widefield fluorescence microscopy and are implementing machine learning to identify and analyze cancer cells on a standard laboratory slide allowing for classic cancer pathology to be applied to circulating tumourderived cells at the single cell level. Well-described hallmarks of cancer can be rediscovered in isolated cancer cells and cell clumps, including aneuploidy, polyploidy, the expression of stem cell markers, mesenchymal makers, epithelial markers, cell proliferation markers, and apoptotic markers, enabling diagnostics based on solid visual evidence. The result is a combined test for CECs and conventional Circulating Tumor Cells (CTCs). Clinical studies on prostate cancer conducted at Siriraj Hospital demonstrated that we could detect CECs and CTCs with high sensitivity and specificity, as well as a high negative predictive value. Based on our research, we have developed a cancer test to support clinical decision making providing urologists with that extra bit of information to make the best decision for their patients. Dr.Prapat Suriyaphol received his Ph.D. in Biochemistry from Mahidol University. His research interests involve bioinformatics, image processing and machine learning. He is head of Division of Bioinformatics and Data Management for Research and a co-founder of X- zell inc. 46 Science and Technology in the Disruptive Era

53 B4_INV002 Sarana Nutanong Scalable Data Systems Lab, School of Information Science and Technology, Vidyasirimedhi Institute of Science and Technology, Rayong 21210, Thailand TOWARDS A DATA-DRIVEN COMPUTATIONAL METHOD FOR GPCRs DRUG DISCOVERY Virtual screening is an essential process in drug discovery concerned with finding lead compounds that is likely to bind to a drug target. This process is achieved by in silico, the computational method of high throughput screening (HTS). Normally, virtual screening is performed by computational biophysics methods such as molecular docking, molecular dynamics simulations. However, these techniques encounter accuracy problems that bring to fail in pre-clinical and clinical process. Recently, machine learning techniques have been presented in drug discovery that allow parameters and model to be learned from experimental data. In this proposed research, G-protein coupled receptors (GPCRs) are key targets for drug treatment with various diseases including obesity, osteoporosis, hyperglycemia, neurodegeneration and cardiovascular diseases. Our work employs integrative computational biophysics and machine learning approaches for finding candidate inhibitors and understanding molecular mechanism between inhibitors and protein targets. These solutions can increase the drug screening accuracy, which can in turn reduce time and cost in pre-clinical and clinical process in drug discovery. Sarana Nutanong, Ph.D. is an Associate Professor at Vidyasirimedhi Institute of Science and Technology (VISTEC). Before joining VISTEC, He was an Assistant Professor at City University of Hong Kong. His research interests include scientific data management, data-intensive computing, spatial-temporal query processing, and large-scale machine learning. More specifically, his research is aimed at providing a large-scale, high-throughput support for computational scientific exploration applications, such as protein simulations and computational drug discovery. 47 The 44 th Congress on Science and Technology of Thailand (STT 44)

54 B4_INV003 Sakda Khoomrung Department of Biochemistry and Siriraj Metabolomics and Phenomics Center, Faculty of Medicine, Siriraj Hospital Mahidol University,2 Prannok Road, Bangkok 10700, Thailand METABOLOMICS FOR THE DEVELOPMENT OF HUMAN VACCINE The advent of omics as genomics, transcriptomics, proteomics, lipidomics, metabolomics alongside with advanced bioinformatic tools are promising technologies for a better understanding of the mechanisms of action of vaccines. Alum has been clinically used as a vaccine adjuvant over past 80 years, however, the impact of alum on host metabolism remains largely unknown. Herein, we have applied MS-based metabolite profiling to study the effects of alum adjuvant on mouse serum at 6, 24, 72 and 168 hours following an immunization with the Mycobacterium tuberculosis vaccine antigen H56 in combination with alum. We employed a novel strategy for class-wise identification of LC-MS-based untargeted metabolomics data called Subclass Identification and Annotation method for Metabolomics (SIAM). Using this approach, we have identified and validated that lipids were the major class of metabolites that significantly changed at 24 hr after alum administration. The majority of these lipids existed in the form of triglycerides alongside with several other unidentified metabolite species. These results may provide new insights into the mode of action of alum adjuvants. Sakda Khoomrung PhD, is an instructor at the Faculty of Medicine, Siriraj Hospital. He graduated from Karl-Franzens University, Austria with a PhD (Dr. rer. nat.) degree in Chemistry in He spent two years ( ) for postdoctoral training and later ( ) became a project leader in the area of analytics and metabolomics at the Systems and Synthetic Biology (led by Prof. Jens Nielsen) at Chalmers University of Technology, Sweden. His primary research interest is to develop a new strategy in metabolomics research combining wet chemistry of old, with state-of-the-art mass spectrometry, and novel bioinformatic tools to understand biological complexity. 48 Science and Technology in the Disruptive Era

55 C1_INV001 Supalax Srijaranai Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand. SECOND GENERATIONS OF DISPERSIVE LIQUID LIQIUID MICROEXTRACTION Dispersive liquid liquid extraction (DLLME), a miniaturized scale of liquid liquid extraction, has become a very popular preconcentration technique since its introduction in 2006 by Assadi and co-workers. DLLME is based on a ternary solvent system including an aqueous solution, a water immiscible solvent (called extraction solvent) and a water miscible solvent (known as dispersive solvent). In DLLME, analytes are extracted into fine droplets of extraction solvent which dispersed in an aqueous solution and after extraction the extraction phase is separated by centrifugation prior to the analysis by appropriate instruments. DLLME offers many advantages such as simplicity, high enrichment factor and rapidity. However, DLLME has some limitations concerning mainly with the requirements related to the extraction solvent, dispersive solvent and extra time consumption for phase separation by centrifugation. This presentation will summarize our previous works investigated for various strategies to overcome those limitations of DLLME. The obtained second generations of DLLME were then applied for the enrichment of organophosphorus pesticide and sulfonylurea herbicide. Prof. Dr. Supalax Srijaranai received B.Sc. (Chemistry) from Khon Kaen Unversity, M.Sc. (Analytical Chemistry) from Chulalongkorn University and Ph.D. (Analytical Chemistry) from University College Cork, Ireland. Her research interests include the development of analytical procedures employing chromatographic and related techniques emphasis on green sample preparation technique for their applications in environment and food samples. 49 The 44 th Congress on Science and Technology of Thailand (STT 44)

56 C1_INV002 Panote Thavarungkul Trace Analysis and Biosensor Research Center, Department of Physics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand ELECTROCHEMICAL CHEMICAL SENSORS AND BIOSENSORS BASED ON POROUS STRUCTURE MATERIALS The ability to detect trace amount of analytes is essential for the control of diseases, food quality and safety, and environment. One key research area is the development of electrochemical sensors and biosensors that can provide fast, selective and sensitive detection. Such research is generally directed towards the improvement of sensing elements and sensors supporting materials. For the latter, the large surface area of porous structure materials plays an important role in enhancing the sensors performance. This presentation demonstrates the application of porous cryogels and metals in chemical sensors as well as catalytic and affinity biosensors. The good selectivity and stability make these electrochemical sensors a potential alternative detection tool. Panote Thavarungkul, D.Phil. Physics (Biophysics) University of Waikato, New Zealand. Associate Professor, Department of Physics, Prince of Songkla University, Thailand. A member of the Trace Analysis and Biosensor Research Center, Prince of Songkla University. Research interest: biosensors and chemical sensors for medical, environmental and industrial applications. 50 Science and Technology in the Disruptive Era

57 C2_INV001 Jaursup Boonmak Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University STRUCTURE-FUNCTION RELATIONSHIPS OF TRANSITION METAL COORDINATION POLYMERS Self-assembled coordination polymers (CPs) have gained extensive attention for not only their fascinating structural diversities but also their applications in many fields, such as gas storage, ion exchange, catalysis, and chemical sensor. The structural assemblies of CPs can be influenced by many factors, including ligand, metal salt, counterion, stoichiometry, temperature, and the ph of the solution. Herein, we will demonstrate the synthesis and X-ray structure-property relationships of transition metal coordination polymers based on various organic linkers. The supramolecular robustness, aniondependent self-assembly of CPs, chemisorption behavior, the single-crystal-to-singlecrystal transformation of CPs induced by external stimuli, and the luminescent coordination polymers for the detection of nitrophenol derivatives will be highlighted in this presentation. Dr. Jaursup Boonmak received his Ph.D. in Chemistry from Khon Kaen University, Thailand. He is currently an assistant professor at Department of Chemistry, Faculty of Science, Khon Kaen University, His research interests include small molecule X-ray crystallography, crystal engineering and functional properties of coordination polymers. His awards include the 2018 Thailand s Young Scientist Award, the Early Career Chemist Award from Pacifichem in 2015, and the Outstanding Research Award from the Thailand Research Fund in The 44 th Congress on Science and Technology of Thailand (STT 44)

58 C2_INV002 Chularat Wattanakit Department of Chemical and Biomolecular Engineering, School of Energy Science and Engineering, Vidyasirimedhi Institute of Science and Technology, Rayong 21210, Thailand. ENANTIOSELECTIVE SYNTHESIS AND SEPARATION AT MESOPOROUS CHIRAL METAL SURFACES Enantioselective recognition and asymmetric synthesis of enantiomers are a crucial scientific challenge due to a large number of potential applications, in particular, sensing and catalysis. We report here the elaboration of chiral imprinted mesoporous metal, obtained by the electrochemical reduction of platinum salts in the simultaneous presence of a liquid crystal phase and chiral template molecules (1). The chiral encoded mesoporous metal perfectly retains the chiral information after removal of the template and shows a very significant discrimination between two enantiomers when using these materials as electrodes in Differential Pulse Voltammetry. Interestingly, such nanostructured metals are also able to break the symmetry during the electrochemical synthesis of mandelic acid as a model molecule (2, 3). The R/S ratio of the synthesis product is higher than unity when using (R)-imprinted electrodes and vice versa for the (S)-imprinted ones. In addition, we could demonstrate that by optimizing the electrochemical synthesis parameters it is possible to achieve very high enantiomeric excess (>90 %) (4). Apart from the enantioselective synthesis, the enantioselective separation has been successfully obtained on chiral platinum films integrated into a microfluidic device. By applying a suitable positive potential to the metal surface, high separation efficiencies were observed due to the favored electrostatic interaction between the negatively charged racemate molecules and the mesoporous metal surfaces. Therefore, these designer surfaces open new promising horizons not only for electroanalysis, but also for efficient enantioselective electrosynthesis and separation. Wattanakit C. would like to thank the research team (Assavapanumat S, Perro A, Sojic A, Garrigue P, Goudeau B, Yutthalekha T, and Kuhn A.) and the research grants supported by VISTEC, French Ministry of Research, CNRS, ENSCBP, the Thailand Research Fund (TRF), Campus France, and the French Embassy in Thailand. Dr. Chularat Wattanakit obtained her Ph.D. in Physical Chemistry from Kasetsart University, Thailand, and University of Bordeaux, France. In 2018, she received the Thailand Young Scientist Award by Foundation for the Promotion of Science and Technology under the Patronage of His Majesty the King. Her research interests relate to the design of heterogeneous catalysts and electrocatalysts for hydrocarbon conversion and asymmetric synthesis. 52 Science and Technology in the Disruptive Era

59 C3_INV001 Tirayut Vilaivan Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok 10330, Thailand QUINOLINE-BASED STYRYL DYES FOR DUAL-MODE COLORIMETRIC AND FLUORESCENCE SENSING OF DNA Styryl dyes consist of two aromatic ring systems with complementary electronic properties linked together via a conjugated π-system, usually a double bond. They have been extensively used as histological staining dyes. Applications of styryl dyes, either alone or in combination with DNA probes for fluorescence sensing of nucleic acids is known, although only limited examples are available. Most importantly, most of previously reports focused on fluorescence sensing. With the aim for developing a simple to use assay for DNA, a series of cationic styryl dyes have been synthesized and their optical properties studied in the absence and presence of single- and double-stranded DNA. It was serendipitously discovered that in addition to over 100-fold fluorescence enhancement, binding of certain quinoline-based styryl dyes resulted in a significant shift of absorption spectra yielding a clear color change visible by naked eyes. The absence of fluorescence and color change in the presence of random proteins such as BSA suggests that the change in optical properties of the styryl dye is specific to DNA binding. This immediately suggests the possibility of using these dyes for DNA sensing purposes. Applications of these dyes, either on their own or in combination with an external probe such as peptide nucleic acid (PNA) to allow sequence-specific detection of DNA with single mismatch resolution will be demonstrated. Tirayut Vilaivan obtained his D.Phil. in Organic Chemistry from Oxford in He is currently a Professor of Chemistry at Department of Chemistry, Chulalongkorn University. His research interests include organic synthesis and its applications as tools to explore the unnatural world. One of his well-known discovery is a new DNA mimic called pyrrolidinyl peptide nucleic acid. He also received several awards, including the prestigious Thailand Outstanding Scientist Award 2014 and TRF Distinguished Research Professor The 44 th Congress on Science and Technology of Thailand (STT 44)

60 C3_INV002 Prasat Kittakoop Chulabhorn Graduate Institute, Bangkok, Thailand. Chulabhorn Research Institute, Bangkok, Thailand. Center of Excellence on Environmental Health and Toxicology (EHT), CHE, Ministry of Education, Bangkok, Thailand. PERSPECTIVE ON NATURAL PRODUCTS CHEMISTRY AND GREEN CHEMISTRY This lecture covers research works on natural products chemistry and green chemistry. Several bioactive compounds have been isolated from fungi and plants; this is from classical natural products chemistry that focuses on the isolation and characterization of bioactive natural compounds. A new approach for the production of new natural product derivatives through one strain-many compounds (OSMAC) method, as well as future trends in natural product research, is given in this lecture. Development of new synthetic methods using green chemistry approach, i.e., an environmentally benign chemical process, is given in this lecture. An organocatalyst prepared from renewable material, e.g., from bovine tendons, could be applied for the synthesis of coumarins and chromenes under solvent-free conditions, and for the synthesis of densely functionalized 4H-chromenes via a threecomponent reaction. A novel method for the generation of nitrile oxides from oxime halides was achieved under mild acidic aqueous solutions (ph 4-5), which could be used for the synthesis of isoxazoles, isoxazolines, and 1,2,4 report on the generation of nitrile oxides from oxime halides under acidic conditions, which is assisted by water. In contrast to the conventional method, the generation of nitrile oxides from oxime halides is in organic solvents under basic conditions. Dr. Prasat Kittakoop received B.Sc. (1st Class Honors, Chemistry) from Chiang Mai University and Ph.D. (Biochemistry) from University of Wales, Swansea (U.K.). His research interests are natural products chemistry, green chemistry, and medicinal chemistry. He published more than 105 papers with more than 3,000 total citations with h-index of 33 (Scopus database). 54 Science and Technology in the Disruptive Era

61 C4_INV001 Nawee Kungwan Department of Chemistry, Faculty of Science, Chiang Mai University, 50200, Thailand and EFFECTS OF DIFFERENT PROTON DONOR AND ACCEPTOR GROUPS ON EXCITED-STATE INTRAMOLECULAR PROTON TRANSFERS OF AMINO-TYPE AND HYDROXY-TYPE HYDROGEN- BONDING MOLECULES: THEORETICAL INSIGHTS The effect of proton donor namely NH-type and OH-type on the excited-state intramolecular proton transfer (ESIPT) of hydrogen-bonding (H-bond) molecules was investigated using density functional theory (DFT) and time-dependent DFT (TD-DFT) at B3LYP with TZVP basis set. The important parameters on bond distances involving the intramolecular H-bond revealed that H-bonds of OH-type are stronger than those of NH-type and supported by the more red-shift of O H -state. vibration The potential energy surfaces along the PT reaction show that ESIPT of O-H type occurs with a small barrier or barrierless in the excited-state whereas those of N-H type have higher PT barrier except the one with stronger proton acceptor, resulting in a smaller barrier. On-thefly dynamics simulations on the first excited-state were further carried out to provide the important dynamic information on PT time and probability. The results of dynamic simulations are accordance with the potential energy surfaces in which the N-H type shows no PT in APBT but slow PT in APBI and fast PT in HNHPIP while O exhibits ultrafast PT within 80 fs. Moreover, the occurrence of ESIPT process are strongly dependent with reaction energy and activation energy, in which the H-bond molecules with thermodynamically and kinetically favorable characters always provide the ESIPT. Therefore, the type of proton donor and proton acceptor of H-bond molecules is very important to hinder or effectively facilitate ESIPT process. References: 1. Chen C-L, Chen Y-T, Demchenko A, Chou P-T. Nature Reviews Chemistry. 2018;2: Kanlayakan N, Kerdpol K, Promin C, Salaeh R, Chansen W, Sattayanon C, Kungwan N. New Journal of Chemistry, 2017;41(17); Dr. Nawee Kungwan received his B.S. degree in Chemistry from Khon Kaen University in 2000 and PhD degree in Chemistry under the supervision of Prof. Thanh N. Troung in 2007 from the University of Utah. He then became a lecturer in the Department of Chemistry, Chiang Mai University in 2007 and did a postdoc research at University of Vienna in His research interests focus on computational chemistry of fluorescent molecule, excited state proton transfer, catalysts for ring opening polymerization and organic dyes for light emitting materials and solar cells. 55 The 44 th Congress on Science and Technology of Thailand (STT 44)

62 C4_INV002 M. Paul Gleeson Department of Biomedical Engineering, Department of Chemistry, College of Nanothechnology, King Mongkut s Institute of Technology Ladkrabang Bangkok, Thailand. paul.gl@kmitl.ac.th APPLICATION OF SYNTHETIC AND COMPUTATIONAL CHEMISTRY METHODS IN ANTI-MALARIAL DRUG DISCOVERY This talk details the theoretical and experimental efforts we have undertaken with the aim of developing new treatments targeting the Plasmodium falciparum (Pf) parasite, a key cause of malaria worldwide. We describe our efforts to develop inhibitors targeting protein kinases as a means to eradicate the Pf parasite. Seventeen new 2,4 diaminopyrimidines analogs were designed, synthesized and tested against the resistant K1 strain of Pf and normal mammalian cells leading to compounds with comparable anti-malarial activity as the drug Chloroquine. We also describe our theoretical studies to elucidate the biochemical mechanism of Dihydropteroate synthase DHPS, a validated anti-malarial target whose efficacy in treating malaria is being increasingly challenged as a result of diseasebased mutations. To date no detailed computational analysis of the protein mechanism has been made. We have employed hybrid QM/MM calculations to assess the energetics associated with SN1 and SN2 processes, identify whether the SN1 process involves a carbocation or neutral DHP intermediate, uncover the identity of the general base in the catalytic mechanism, and understand the differences in substrate vs. inhibitor reactivity. References: 1. Chotpatiwetchkul, W.; Boonyarattanakalin, K.; Gleeson, D.;Gleeson, M. P. Org. Biomol. Chem., 2017, 15, Phuangsawai, O.; Beswick, P.;Ratanabunyong, S.; Tabtimmai, L.; Suphakun, P.; Obounchoey, P.; Srisook, P.; Horata, N.; Chuckowree, I.; Hannongbua, S.; Ward, S. E.; Choowongkomon, K.; Gleeson, M. P. Eur. J. Med. Chem., 2016, 124, 29, Science and Technology in the Disruptive Era

63 D_INV001 Jakrapong Kaewkhao Physics Program, Faculty of Science and Technology, Nakhon Pathom Rajabhat University, Nakhon Pathom, 73000, Thailand FAST DECAY TIME OF CE 3+ DOPED IN SODIUM-GALDOLINIUM- ALUMNIUM-PHOSPHATE FOR SCINTILLATION MATERIAL APPLICATION The Ce 3+ doped sodium-gadolinium-aluminum-phosphate glasses, with 20Na 2O 10Gd 2O3 10Al 2O3 (60-x)P 2O5 xcef 3 (where x = 0.00, 0.10, 0.50, 1.50, 2.00 and 3.00 mol%) composition, have been measured and analyzed. The prepared glasses were studied various properties such as physical, optical, luminescence and scintillation. The densities of glasses were found to increase with the concentration of CeF 3 while molar volumes decreased. The value of absorption edge is shifted from 313 nm of host glass to longer wavelength with CeF 3 doping. The indirect and direct band gap decreased with increasing CeF 3 content. Photoluminescence spectra showed the strong emission bands centered between nm under Ce 3+ directly excitation and Gd 3+ excitation. The optimum concentrations of CeF 3 concentration in this glass is 2.00 mol% as it resulted the maximum emission intensity. The strongest emission band of x-ray induced optical luminescence is around 375 nm. The integrated scintillation efficiency of 2.0 mol% CeF 3 doped glass was 9.6% compared with bismuth germanium oxide (BGO) single crystal. The most dominant decay times of 2.0 mol% doped glass is 26.8 ns that performed the rapid response with coming excitation energy. In this work, our developed glasses performed the strong and fast luminescence signal which can be applied as the scintillation material in radiation detector. Assoc.Prof.Dr.Jakrapong Kaewkhao received Ph.D. degree in physics from King Mongkut s University of Technology Thonburi (KMUTT), Thailand, in He attended a postdoctoral research of an x-rays induced luminescence study in glasses at KyungPook National University (KNU), Korea, in In the same year, he has been awarded as the best alumni of Silpakorn University, Thailand. 57 The 44 th Congress on Science and Technology of Thailand (STT 44)

64 D_INV002 Anoma Thitithammawong Department of Rubber Technology and Polymer Science, Faculty of Science and Technology, Prince of Songkla University, Pattani Campus, Pattani 94000, Thailand MODIFIED CASHEW NUT SHELL LIQUID AS CURING AGENT AND ORGANO-MODIFIER IN LDH FILLED NATURAL RUBBER Cashew nut shell liquid (CNSL), a by-product from the cashew industry, is a renewable natural material and has as its advantages cheap price, abundant availability, and biodegradability. The natural raw CNSL mainly composes of phenolic compounds with different alkenyl side chains. The acidic chemicals could retard crosslinking in rubber vulcanization by absorbing accelerators. On the other hand, the phenol component can boost oxidation resistance. Isolating the components of CNSL is not a cost effective option, and it would also involve organic solvents that are not environmentally friendly. Therefore, the use of CNSL in rubber needs to balance between the various effects. In the present work, the effect of modified CNSL on properties of NR compounds and NR vulcanizates was investigated in comparison with raw CNSL. The properties in terms of curing characteristic, crosslink density, physical properties, and thermal stability, were characterized and are discussed. In addition, the possibility of using CNSL as surface modifier of layered double hydroxides (LDH) - anionic clay was investigated. Chemical and crystal structures of organo- LDH were confirmed. Morphological study and mechanical tests were also studied in organo-ldh filled NR composites. Anoma Thitithammawong is currently an assistant professor at Faculty of Science and Technology, Prince of Songkla University, Pattani campus. She also shares her knowledge as a consultant and coach for many Thai s rubber community enterprises and rubber industries. This year she had received the Rising Star Award 2018 from Polymer Society of Thailand. Her research focuses on the use of a renewable natural material as a positive agent to boot properties of rubber vulcanizates and rubber composite. 58 Science and Technology in the Disruptive Era

65 D_INV003 Tosapol Maluangnont Electroceramics Research Laboratory, College of Nanotechnology, King Mongkut s Institute of Technology Ladkrabang, Bangkok 10520, Thailand tosapol.ma@kmitl.ac.th GIANT DIELECTRIC CONSTANTS IN LEPIDOCROCITE-TYPE TITANATE WITH INTERLAYER ALKALI IONS AND EDGE-SHARED TIO 6 MOTIFS Several titanium-containing oxides with interesting dielectric properties contain mostly (if not all) corner-shared TiO 6 polyhedra. In contrast, the dielectric properties of materials containing edge-shared TiO 6 motifs have been underexplored. Here, we presented structural and surface characterizations, including dielectric properties in the lepidocrocitetype layered titanate A xti 2- ym yo 4 where A + = K +, Rb +, Cs + ; x = ; y = (4-x)/2; M = Zn (see Fig. 1, left). In this structure, the interlayer cations interleaved between the negativelycharged sheets constructed from the edge-shared (Ti,M)O 6 units. Taking K 0.8Ti 1.6Zn 0.4O4 as an example, the maximum dielectric constants ε max ~10 4 and the dielectric losses tan δ ~ were obtained at 10 3 Hz, upon heating from RT to 250 C (Fig. 1, right). The results were explained considering (i) Maxwell-Wagner polarization of A +, and (ii) the formation of internal barrier layer capacitors (IBLCs) via the oxidized surfaces. Further engineering the dielectric properties via compositional tuning, or and by microstructure optimization, has also been demonstrated. The two-dimensionality (2D) of this material clearly allows unique chemistry unlike that of typical 3D counterparts, including the possibility for fabricating ultra-thin capacitors. The Ce 3+ doped sodium-gadolinium-aluminum-phosphate glasses, with 20Na 2O 10Gd 2O3 10Al 2O3 (60-x)P 2O5 xcef 3 (where x = 0.00, 0.10, 0.50, 1.50, 2.00 and 3.00 mol%) composition, have been measured and analyzed. The prepared glasses were studied various properties such as physical, optical, luminescence and scintillation. Tosapol Maluangnont is currently an assistant professor at College of Nanotechnology, King Mongkut s Institute of Technology Ladkrabang. His research focuses on understanding the chemical and physical properties of alkali titanate, including the nanostructures derived therefrom, for renewable and sustainable energy. He was the successful candidate for the Professional Development for Mid-career Researchers granted by Newton Fund and Thailand Research Fund (2016), and the research fellow of the Hitachi Global Foundation (2017). 59 The 44 th Congress on Science and Technology of Thailand (STT 44)

66 E_INV001 Kraichat Tantrakarnapa Department of Social and Environmental Medicine Faculty of Tropical Medicine, Mahidol University THE HAZE EPISODE IN THE NORHTERN PART OF THAILAND AND THE SYSTEM APPROACH FOR FACING THE SITUATION RIGHT, ALL CAPS) The HAZE episode is one aspect of environmental problem of the Southeast Asia countries, it has been detected for more than 10 years. It is an additional burden affecting human health, socio-economics and social activities. The haze is one important environmental problem in Thailand, it is happened in the different period depends upon the topography and location. Haze episode in the upper north of Thailand, normally, it has been found during the dry season. This research aims to gain insights the haze situation and influencing factors. The obtained data of air quality from Pollution Control Department was used for analysis. The forecasting system was developed for prediction, the forecasting can be illustrated for three days from the existing date. The integrating approach from various disciplinary was employed for solving the problem, the five categories of research project have been created namely; (1) Supply chain management of alternative agricultural products (2) Health impacts (3) Haze dispersion modeling and health impacts (4) Monitoring system development and, (5) Curriculum development for haze management. The results of the first year prediction system indicated that the AQI (Air Quality Index) generated from the model is similar to the monitoring data obtained from Pollution Control Department. The correlation coefficients of observed data and modeling data were calculated, it was found that the correlation were moderate to high level ( ). The first year results indicated that the haze episode solution is complicated and need more collaboration among the stakeholders. Number of hot spots in the first year (2017) was decreased compare to the previous years. In addition, the exceeded particulate matter concentration (PM10) days were also decreased in the concern areas. References: 1. (climate and air quality data) Kraichat Tantrakarnapa, Associate Professor (Research interest: Environmental Health). Education: BS. (Statistics), MSc. (Technology of Environmental Management), PhD. (Environmental Engineering), Post graduate in Occupational Health and Safety in the Workplaces. Award: Outstanding Contribution Award to Environmental and Health of Asia from Korean Society of Environmental Health 60 Science and Technology in the Disruptive Era

67 F_INV001 4 x 3 cm photo Thiti Bovornratanaraks Extreme Conditions Physics Research Laboratory, Physics of Energy Materials Research Unit, Department of Physics, Faculty of Science, Chulalongkorn University thiti.b@chula.ac.th SCIENCE UNDER HIGH PRESSURE: THE NEW ALCHEMISTS Over the past decade, the pressure became a vital variable which governs both the physical and chemical properties of materials. The relation between thermodynamically stable and superconductivity preparation is one of the fundamental questions in physics, geophysics, and chemistry. Since the discovery of the novel structure, this has remained as one of the main questions regarding the very foundation of elemental metals and simple materials. Needless to say, this has also bearings on extreme conditions physics, where again the relation between structure and performance is of direct interest. In this talk, the review on high-pressure research on several advance materials will be given. Covering from energy material such as perovskite solar cell, super hard materials, and Li battery to the food and drink products. Thiti Bovornratanaraks is an associate professor at the Department of Physics, Chulalongkorn University, where he has devoted his time to teaching Physics for students of various discipline as well as carried out several research projects on High Pressure Physics for the last twenty years. He is co-author of more than 30 international peer-reviewed journals and more than 20 scientific conference contributions. He is also a policy fellow at the STI. 61 The 44 th Congress on Science and Technology of Thailand (STT 44)

68 G_INV001 Nipon Parinyavuttichai Department of Information Technology, Faculty of Science and Technology, Rajabhat Rajanagarindra University ARE WE READY FOR THE AGING SOCIETY? A CASE STUDY OF CHA-CHOENG-SAO TOWN MUNICIPALITY In Thailand, more people are living longer than ever before. On the one hand, this means a vast increase in a valuable resource to society: older people who volunteer their time in the community and who are able to meaningfully share their culture and values with subsequent generations. But, on the other hand, families and communities will have seriously limited resources to look after aging parents. This presentation, as the first step to prepare for the situations, will adopt information theories in order to investigate and analyze elderly situations in Cha-choeng-sao Town Municipality, and further provide suggestions on how local legislators and politicians could improve the situations through policy development and implementation. In addition, the presenter will illustrate how technological advancement could be applied to confront with such challenges. And finally what we, as members in this sharing society, should be prepared for a better tomorrow. Dr. Nipon Parinyavuttichai is currently an Assistant Professor at the Department of Information Technology, Faculty of Science and Technology, Rajabhat Rajanagarindra University. Dr. Parinyavuttichai received B.Eng. in Computer Engineering from KMITL. After the graduation, Dr. Parinyavuttichai went to Old Dominion University, U.S.A. to study MSc. In Computer Information Science, a joint program between Computer Science and MBA., with the Royal Thai Government scholarship. Dr. Parinyavuttichai pursued his studies in Information Studies at The University of Sheffield, U.K., accepted another Royal Thai Government scholarship. In 2011, Dr. Parinyavuttichai was awarded PhD. from the ischool of the University of Sheffield. Dr. Parinyavuttichai s research interests include information management, information systems development (ISD) project, locality development and improvement. Earlier this year, Dr. Parinayuttichai had been recognized by the Thai Health Promotion Foundation and Knowledge Center of Cha-cheong-sao (KCC) for his work on a volunteer engagement program in the Cha-choeng-sao municipality of Thailand. 62 Science and Technology in the Disruptive Era

69 G_INV002 Marut Buranarach National Electronics and Computer Technology Center (NECTEC) 4 x 3 cm photo TOWARDS OPEN INNOVATION WITH OPEN DATA SERVICE PLATFORM Open government data (OGD) is a global initiative to promote transparency, service innovation and citizen participation. The most common means for publishing OGD is usually in forms of datasets made available on OGD catalogs. Although publishing open data as datasets is straightforward and requires minimal technological skills, it is not ideal for the users who want to use the data in a more dynamic fashion. This talk presents a framework for a software platform that can create OGD services from OGD catalog. Our framework emphasizes the need to convert existing OGD datasets to RDF data and value-added services that provides more convenient access to the users. Data APIs and visualization tools are among the OGD services to promote utilization of the OGD datasets. Our framework is unique in that it hides the complexity of the Semantic Web technology from the data publishers and users. In this framework, the OGD datasets are validated for its well-formedness of the tabular data. The service building system converted the data to the RDF format and utilized SPARQL query templates in building the OGD services for each dataset. The framework was applied with the datasets on Data.go.th. The case study exemplifies a semi-automatic approach to augmenting access to OGD and provide a first step in moving OGD towards linked data. Dr. Marut Buranarach received his B. Eng., (Telecommunication Engineering) from King Mongkut s Institute of Technology Lardkrabang and M.S. and Ph.D. (Information Science) from University of Pittsburgh, USA. His research interests Semantic Web Technologies, Ontology, Knowledge Management, Database Management. His current research projects focus on Open Government Data as a Service Platform Excise Product Data Taxonomy and Excise Duty Recommender System for the Excise Department. 63 The 44 th Congress on Science and Technology of Thailand (STT 44)

70 G_INV003 Akadej Udomchaiporn Department of Computer Science, Faculty of Science, King Mongkut s Institute of Technology Ladkrabang akadej.ud@kmitl.ac.th 3-D MRI BRAIN SCAN CLASSIFICATION: EPILEPSY VERSUS NON- EPILEPSY 3-D classification methods are presented for application to Magnetic Resonance Imaging (MRI) brain scan volumes. The objective of the classification is to identify volumes that feature indicators of epilepsy against volumes that do not, by considering the shape and size of the lateral (left and right) ventricles of the brain. The dataset used with respect to the reported experimentation comprised D MRI brain scans of which 240 were from epilepsy patients and the remainder from healthy people. The classification is supported by four proposed representation techniques: (i) Statistical Based, (ii) Point Series Based, (iii) Tree Based, and (iv) Deep Learning Based. The reported experimental results indicated that the proposed techniques can classify 3-D MRI brain scan data with a classification accuracy of up to 93%. Dr.Akadej Udomchaiporn is a lecturer at Department of Computer Science, Faculty of Science, King Mongkut s Institute of Technology Ladkrabang (KMITL). He received B.Sc. in Computer Science from KMITL, M.Sc. in Software Engineering from Chulalongkorn University, M.Sc. in Advanced computer Science, and Ph.D. in Computer Science from University of Liverpool. His research interests are Medical Image Mining, Satellite Image Mining, and Business Data Analytics. 64 Science and Technology in the Disruptive Era

71 G_INV004 Chalump Oonariya Thai Meteorological Department UNDERSTANDING THE LINK BETWEEN CLIMATE CHANGE AND EXTREME WEATHER EVENTS An overview of global climate change will be presented. Under working group-i of the Intergovernmental Panel on Climate Change (IPCC) shown that warming of the climate system is unequivocal and human influence on the climate system is clear because of the greenhouse gases emission. Most of the exceed energy is stored in the ocean and influence on the atmospheric and oceanic activities. One conclusion of the working group points out that the extreme weather is typically rare, but climate change is increasing the odds of more extreme weather events taking place. To be understanding the link between climate change and extreme weather events, many key scientific knowledges will be addressed: 1) The characteristics and physical mechanisms of the weather and climate variations of the global and regional areas caused by climate change. 2) The physical mechanisms are linking of the weather and climate extreme events under climate change impacts. 3) The most predictable patterns of the weather and climate variations lead to the extreme events are still unopened and challenging. The scientific evident shown that the extreme weather events are more intense and frequency in the last decade. The researchers are now on the same page to investigate and put the physical scientific basis on them. Chalump Oonariya is a scientist at Thai Meteorological Department. His work now focuses on computational fluid dynamics, the atmospheric and oceanic circulations and its impacts under climate change leads to extreme weather events. He earned a doctorate in theoretical physics under the DAAD Thai-German Sandwich Programmed at Suranaree Univerisity of Technology and Eberhard Karls Universität Tübingen, Germany. In , he received a postdoctoral research fellow and become a research scientist at NOAA Science Center for Weather and Climate Prediction in Maryland, USA. 65 The 44 th Congress on Science and Technology of Thailand (STT 44)

72 G_INV005 4 x 3 cm photo Chayanan Nawapornanan Office of Information Technology Thai Beverage Public Company Limited 333 Lao Peng Nguan Tower Soi Choeipuang, Vibhavadi Rangsit Rd., Chompol, Bangkok Thailand chayanan.n@thaibev.com AN INCREMENTAL MINING ALGORITHM FOR SHARE FREQUENT ITEMSETS In the real world application, new transactions are usually incrementally inserted into a database. Most share frequent itemsets algorithms need to re-mine the whole updated database to meet the results. In this research, we proposed a new efficient algorithm for mining share frequent itemset on incremental database, called Incremental Share Frequent Itemset Mining, or IS-FUP, for short. It is extended from the MCShFI algorithm in a batch way with Fast Update Concept (FUP). IS-FUP eliminates the rescanning by mining the share frequent itemset only from new transactions. As a result, the previously discovered share frequent itemset are updated efficiently. Extensive performance analyses show that the execution time of the IS-FUP algorithm outperforms those of the batch way on both synthetic datasets and real-life datasets. References: 1. Agarwal, R. and R. Srikant. "Mining sequential patterns: generalizations and performance improvements." Proceedings of 5th International Conference on Extending Database Technology Agarwal, R. and R. Srikant. "Fast Algorithms for Mining Association Rules in Large Databases." Proceedings of 20th International Conference on Very Large Data Bases "Mining sequential patterns." Proceedings of 11th International Conference on Data Engineering Agarwal, R., C. Aggarwal and V. V. V. Prasad. "A tree projection algorithm for generation of frequent itemsets." Journal of Parallel and Distributed 61 (2001): Agarwal, R., T. Imielinski and A. Swami. "Mining Association Rules Between Sets of Items in Large Database." Proceedings of the ACM SIGMOD on Management of Data Chayanan Nawapornanan works as an Assistant Manager of Office of Business Intelligence Development at Thai Beverages Public Company Limited. She received the B.Tech. degree and the M.Sc. degree in Information Technology from Burapha University, Thailand. She also received the M.Sc. degree and the Ph.D. degree in Computer Science from KMITL, Thailand. Her research interests include knowledge discovery in databases (data mining) and machine learning. 66 Science and Technology in the Disruptive Era

73 H_INV001 Anadi Nitithamyong Senior Advisor, Institute of Nutrition, Mahidol University and President, Food science and Technology Association of Thailand (FoSTAT) DESIGNING FOODS FOR THE SILVER GENERATION: AN INNOVATION CHALLENGE As the number of aging population in the world continues to increase there is a constantly growing demand for foods that are suitable for their specific conditions. When a person advances in age, physiological as well as psychological changes occur throughout one's life. Such changes include significant loss in muscle mass, reducing bone density, tendency to become easily dehydrated, and more importantly changes in the digestive system and decreased in efficiency in sensory perception. All these factors markedly affect the elder's ability to consume foods and obtain optimum nutrition for their health and wellbeing. One of the key reasons is the lack of foods or food products that are satisfactory to their requirement and at the same time can be attractive enough to enhance their declining appetite. Therefore, challenges exist to design foods that would help to resolve these problems. Food science and technology can play a key role in creating innovative foods for the above purposes. The presentation will cover ageing society situation, physiological changes and nutrition requirements in ageing population and current technology available to promote innovations of foods and food products for the silver generation. Assistant Professor Anadi Nitithamyong received B.Sc. (Food Technology) from Chulalongkorn University, M.Sc. and Ph.D. (Food Science) from University of Wisconsin- Madison, USA. Her research interests include the food processing and food product development for nutritional purpose. 67 The 44 th Congress on Science and Technology of Thailand (STT 44)

74 H_INV002 Parichat Hongsprabhas Department of Food Science and Technology, Faculty of Agro- Industry, Kasetsart University, Bangkok 10900, Thailand BIOFUNCTIONAL AND PHYSICOCHEMICAL PROPERTIES OF RECONSTITUTED OKRA MUCILAGE-MALTODEXTRIN SPRAY- DRIED POWDER Okra (Abelmoschus esculentus (L.) Moench) pod is commonly consumed as raw or cooked vegetable in Thailand. The objectives of this study were to elucidate biofunctional and physicochemical characteristics of reconstituted okra mucilage co-dried with cassava maltodextrin (SO) prepared by spray-drying process. The expression of THP-1 monocyte inflammatory genes, IL-1β, IL-10 and TNF-α indicated that reconstituted SO had proinflammatory activity but did not have anti-inflammatory activity. The flow behaviour of reconstituted SO was found to be concentration-dependent. At low SO concentration as 4% (w/v), the flow behaviour of reconstituted SO was similar to that of 3% (w/v) porcine mucin with mix behaviour of Herschel-Bulkly fluid at low shear rate that changed to Bingham fluid at shear rate above 1 s-1 (P<0.05). At the SO concentration between 7 27% (w/v), reconstituted SO showed single flow behavior of Bingham fluid with high yield stress (P<0.05). The addition of porcine mucin (0 1.2 % (w/v) did not alter flow behavior of reconstituted 4% (w/v) SO (P>0.05). However, hydrolysis of cassava maltodextrin present in the reconstituted SO-mucin mixtures by α-amylase resulted in the mixed fluids having higher yield stress (P<0.05) after starch digestion, suggesting the increase in thickening ability of mixed starch hydrolysate and mucin after α-amylase hydrolysis. Pseudo first order kinetic model was used to estimate the content of glucose released over the 152 min period of in vitro digestion from oral to intestinal phases of cooked rice in the absence or presence of SO. Starch digestion kinetics parameters, namely glucose concentration at end point (C ), pseudo first order rate constant (k), and half-time (t0.5) revealed that the presence of SO prior to digestion did not have significant effects on in vitro glucose release of cooked rice of different cultivars containing intermediate amylose content (P>0.5). Overall, this study showed the potential use of okra mucilage water-extract powder as thickener for the bolus formation of diets containing cooked rice grains. Parichat received her Ph.D. in Food Science from the University of Guelph in She joined Kasetsart University in 2003 and is currently an associate professor at Kasetsart University. Parichat teaches colloidal systems in foods, food materials and food protein chemistry for graduate courses. Her current researches emphasize on food and biomaterial innovation and the integration of food physics to human health. 68 Science and Technology in the Disruptive Era

75 SP1_INV001 Satoshi Imura National Institute of Polar Research, Japan POLAR SCIENCE OF JAPAN Sixty years have passed since the International Geophysical Year (IGY) and also since the First Japanese Antarctic Research Expedition (JARE) was deployed to the Antarctic in It has also been a quarter of a century since Japan s National Institute of Polar Research (NIPR) opened its Arctic research station in Svalbard, Norway in Recent research into Earth s environmental changes and global warming has progressed, and Japan s research of the Antarctic and Arctic regions is now more relevant than before. Founded in 1973, NIPR is an inter-university research institute that conducts comprehensive scientific research and observations in the polar regions. Currently, NIPR conducts observations and research with other countries under the frameworks of various academic organizations such as the Scientific Committee on Antarctic Research (SCAR), the International Arctic Science Committee (IASC), and the Scientific Committee on Solar-Terrestrial Physics (SCOSTEP) under the International Science Council (ICSU). Collaboration with Thailand in polar science is one of our representative activities under this frameworks. Ph.D., Science, Univ. of Hiroshima, 1992 Professor, Vice Director-General, Head of International Affairs and Research Development Office. Biologist, focusing on terrestrial ecosystem in Polar region, especially interested in biodiversity and reproduction of mosses, and systems of lake bottom vegetation. 69 The 44 th Congress on Science and Technology of Thailand (STT 44)

76 SP1_INV002 Hyoung Chul Shin Korea Polar Research Institute KOREAN POLAR RESEARCH IN 30 YEARS; RETROSPECT AND PROSPECT The inauguration of the King Sejong Station on King George Island off the coast of Antarctic Peninsula in 1988 marked the genesis of Korean polar research. As the lead agency for Korea s national Polar program for both the Arctic and the Antarctic, the Korea Polar Research Institute commemorated the 30th anniversary of the station s establishment this year. In its early ears, traditional research fields encompassing earth and life sciences formed the backbone of Korean polar research, which has now expanded to include emerging fields as well as new terrains and waters, while making good use of new technologies. Many scientific achievements have been made through atmospheric observations, geological discovery, paleoenvironment reconstruction, biodiversity and ecosystem studies, and metabolite and genomic research in the Antarctic Peninsula region. Arctic science has also become an important part of Korea s research portfolio since the establishment of the Dasan Station in Ny-Ålesund, Svalbard, in 2002, and received a further boost when a research icebreaker, Araon was commissioned in Currently, Korean polar research focuses on three major areas: understanding the role of the Antarctic in the global system; accumulating knowledge and information for future Arctic needs; and newly emerging frontier science. The current status of Korean polar science is now regarded as advancing into a developed stage. Over the next 10 years, Korean polar scientists aim to undertake truly global polar research. This should manifest in farreaching scientific findings of great significance and in the breadth and depth of international partnership. Collaborative science in key areas such as Antarctic waters of rapid glacial retreat, subglacial lakes, or the central Arctic with rapidly diminishing sea ice will be good examples of such research. Securing and efficiently managing infrastructure and logistical support such as a research icebreaker of adequate ice capability as well as Antarctic inland paths will be critical. Science diplomacy and the translation of research findings into effective policies will be among the most important features and indicators of fully developed and truly global polar science research. Dr. Hyoung Chul Shin, a biological oceanographer by training, participated in and coordinated numerous expeditions to the Antarctic and the Arctic. He dedicates much of his time to science as well as science support. His activities and interests include the management of marine living resources in polar waters. From 2013 until very recently, he served as the Vice Chair of the Council of Managers of National Antarctic Programs (COMNAP) and as one of the Vice Chairs of the Forum of Arctic Research Operators (FARO). 70 Science and Technology in the Disruptive Era

77 SP1_INV003 Kim Holmén Norwegian Polar Institute THE NORWEGIAN POLAR INSTITUTE FROM POLE TO POLE The Norwegian Polar Institute (NPI) is celebrating its 90th anniversary in Initially the focus was on mapping the Arctic with an emphasis on geological resources in Svalbard. In the middle of the last century NPI was also tasked with investigating Antarctica. Simultaneously the portfolio of investigation was modified both in the north and the south to focus on environment. This includes acquiring a fundamental understanding of the processes determining the state of the environment both in the physical and biological and their mutual interactions. The Norwegian polar regions include Svalbard and Jan Mayen with adjacent seas, Bouvet Island in the South Atlantic, Peter I Island and Dronning Maud Land in Antarctica. The presentation will include a number of examples of present research activities in these areas under the auspices of NPI. The examples will include recent developments in climate change research and the effects of climate change on biodiversity. Ph.D in Meteorology at Stockholm University, Sweden Member of the Royal Swedish Academy of Forestry and Agriculture Present position: International director at Norwegian Polar Institute, Tromsø, Norway Present. Research interests are within the fields of human induced climate change and biogeochemical cycles. Elucidating climatic interactions with the biogeochemical cycle of carbon on different temporal and spatial scales through process studies and modelling remains a key activity. Field-work experience includes over 30 years of climate monitoring activities in Ny-Ålesund, Svalbard, as well as expeditions to Antarctica and Siberia. Recent work also addresses the role of science in policy and ethics. 71 The 44 th Congress on Science and Technology of Thailand (STT 44)

78 SP1_INV004 Voranop Viyakarn Chulalongkorn University & Polar Sciences Consortium of Thailand POLAR SCIENCES OF THAILAND The Polar Sciences Consortium of Thailand (PSCT) is recently established in 2017 under the Information Technology Foundation under the Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn (Princess Sirindhorn IT Foundation). The Princess Sirindhorn IT Foundation is initiated by Her Royal Highness Princess Maha Chakri Sirindhorn to improve the quality of life and future outlook of Thai citizens. Under the Princess Sirindhorn IT Foundation, HRH Princess has initiated many development projects. Most of them are facilitated by and working in coordinating with various agencies, organizations, universities, both governmental and private sectors. In 2013, Her Royal Highness has initiated the Polar Research Program under the Princess Sirindhorn IT Foundation, and has started a regular dispatch of scientists for polar research expeditions in Antarctic and Arctic with other countries. Later in 2017, PSCT is established to overlook, facilitate, and providing funds for Thai polar research activities. In Thailand, the history of visiting Antarctic was started in 1993 when Her Royal Highness was the first Thai to visit there. Then, Thailand started to dispatch Thai scientists to Antarctic for research exploration for the first time in 2004 with the Japanese Antarctic Research Expedition (JARE), and later in 2014, has a regular dispatch with the Chinese Antarctic Research Expedition (CHINARE). For the Arctic research, Thailand has successfully finished the first Arctic Expedition in At present, Thailand is an associate member of the Scientific Committee on Antarctic Research (SCAR) and a member of the Asian Forum for Polar Sciences (AFoPS). Education: - Ph.D. (Fish.Sc.), Tokyo University of Fisheries, Japan, 1993 Present Position: - Associate Professor & Head Department of Marine Science, Faculty of Science, Chulalongkorn University - Vice Chair, Polar Sciences Consortium of Thailand - Deputy Director for Academic Affairs, Thai Sea and Island Natural History Museum, Plant Genetic Conservation Project under Her Royal Highness Princess Maha Chakri Sirindhorn Royal Thai Nany Research Interest: - Diversity of marine organisms and impact of climate change and human disturbances on marine organisms both in tropical and polar regions 72 Science and Technology in the Disruptive Era

79 SPECIAL SESSION (SP1: THAI ANTARCTIC-ARCTIC EXPEDITIONS) SP1_INV005 Suchana Chavanich Department of Marine Science, Faculty of Science, Chulalongkorn University Year to Antarctica: 2009 and 2014 With JARE/CHINARE: JARE 51 and CHINARE 30 Research topic in Antarctica: Impact of climate change on marine organisms and environment SP1_INV006 Onruthai Pinyakong Department of Microbiology, Faculty of Science, Chulalongkorn University Year to Antarctica: 2014 With JARE/CHINARE: CHINARE 30 Research topic in Antarctica: Microbial diversity and functions in Antarctic areas SP1_INV007 Anukul Buranapratheprat Department of Aquatic Science, Faculty of Science Burapha University Year to Antarctica: 2015 With JARE/CHINARE: CHINARE 31 Research topic in Antarctica: Water column conditions and deep water mass formation in Prydz Bay 73 The 44 th Congress on Science and Technology of Thailand (STT 44)

80 SP1_INV008 Prayath Nantasin Department of Earth Sciences, Faculty of Science Kasetsart University Year to Antarctica: 2016 With JARE58 Research topic in Antarctica: high grade metamorphic rocks Lutzow- Holm Bay and Amundsen Bay, East Antarctica. SP1_INV009 Pataporn Kuanui True corporation Year to Antarctica: 2016 With JARE/CHINARE: CHINARE 32 Research topic in Antarctica: The impact of climate change on marine communities in Antarctica SP1_INV0010 Pitsanupong Kanjanapayont Department of Geology, Faculty of Science, Chulalongkorn University Year to Antarctica: 2017 With CHINARE 33 Research topic in Antarctica: Geological study of Antarctica (Great Wall Station) and its tectonic evolution 74 Science and Technology in the Disruptive Era

81 SP1_INV0011 Thasinee Charoentirat Department of Geology, Faculty of Science, Chulalongkorn University Year to Antarctica: 2017 With JARE/CHINARE: CHINARE 33 Research topic in Antarctica: Geological study of Antarctica (Great Wall Station) and its tectonic evolution. SP1_INV0012 Kittiphop Promdee Department of Environmental Science, Academic Division, Chulachomklao Royal Military Academy Year to Antarctica: 2018 With CHINARE 34 Research topic in Antarctica: The study of environmental change and atmospheric phenomena in Antarctic using GNSS SP1_INV0013 Teetat Charoenkalunyuta Department of Survey Engineering, Faculty of Engineering, Chulalongkorn University Year to Antarctica: 2018 CHINARE 34 Research topic in Antarctica: The study of environmental change and atmospheric phenomena in Antarctic using GNSS. 75 The 44 th Congress on Science and Technology of Thailand (STT 44)

82 SP1_INV0014 David Ruffolo Department of Physics, Faculty of Science, Mahidol University Year to Antarctica: Analyzed data from 1989, 2000, 2001, 2003, 2005, 2017 With JARE/CHINARE: --- Research topic in Antarctica: Relativistic ions from solar storms, etc. SP1_INV0015 Alejandro Sáiz Mahidol University Research topic in Antarctica: Cosmic ray and solar energetic particle flux and spectrum recorded by polar neutron monitors SP1_INV0016 Waraporn Nuntiyakul Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Year to Antarctica: Analyzed data from and With JARE/CHINARE: Research topic in Antarctica: The data from neutron monitors located in Antarctica and at the South Pole. For more information, please visit 76 Science and Technology in the Disruptive Era

83 SP2_INV001 Akkharawit Kanjana-opas Deputy Secretary General, National Science Technology and Innovation Policy Office (STI) and Chief Executive Officer of Food Innopolis SURVIVING AND MANAGING CHANGES IN THIS DISRUPTIVE ERA: HOW ABOUT THAILAND? Before joining STI, Dr. Akkharawit Kanjana-Opas had been responsible for the intellectual property management and technology transfer, business incubation as well as the collaborative research of the Prince of Songkhla University Science Park during Currently, he is responsible for the New Economy Development based on Science, Technology and Innovation as well as the development of Food Innopolis which is the national initiative for food innovation ecosystem in Thailand. Dr. Akkharawit received his Ph.D. in Marine Chemistry from Scripps Institution of Oceanography, University of California San Diego. Upon the completion of this graduate study, he has participated in several trainings in the field of intellectual property and technology transfer in Japan, Germany, Italy, Sweden, Singapore, and Thailand. He is also certified as Thai professional patent agent by the Department of Intellectual Property, Thailand. He teaches and conducts seminars related to intellectual property in various universities and institutions in Thailand and other ASEAN countries. 77 The 44 th Congress on Science and Technology of Thailand (STT 44)

84 SP2_INV002 Chinawut Chinaprayoon Vice President of Digital Startup Promotion Institute Digital Economy Promotion Agency SURVIVING AND MANAGING CHANGES IN THIS DISRUPTIVE ERA: HOW ABOUT THAILAND? Dr. Chinawut is currently the Vice President and Director of Digital Startup Promotion Institute at Thailand s Digital Economy Promotion Agency (DEPA). Before joining DEPA, he was the Director of New Venture & Entrepreneurship at National Science Technology and Innovation Policy Office. His previous roles included the setting up of Startup Thailand platform as well as the launching of new laws and regulations to support new breeds of innovative entrepreneurs. Dr. Chinawut holds MS in Science and Technology Policy from Georgia Institute of Technology, USA and PhD in Innovation Management from Rensselaer Polytechnic Institute (RPI) in New York. He was also a serial-entrepreneur, successfully building a few innovative travel enterprises. 78 Science and Technology in the Disruptive Era

85 SP2_INV003 Lena Ng Chief Investment Officer Amata Corporation Public Company Limited 2126 Kromadit Building, New Petchburi Road, Bangkok SURVIVING AND MANAGING CHANGES IN THIS DISRUPTIVE ERA: HOW ABOUT THAILAND? Ms. Lena Ng currently acts as the Chief Investment Officer for Amata Corporation Public Company Ltd and oversees strategic investments, mergers & acquisitions and smart cities development. She is also the Director of Amata Global Pte Ltd in Singapore. Lena was honored as a Young Global Leader by the World Economic Forum in March 2014 and nominated as a Global Agenda Council Member of World Economic Forum. Prior to her current appointments, Lena was also the Chairperson (Audit Committee) and Board Member of the Singapore National Paralympic Council. She was a former Singapore Diplomat (Commercial Attaché) based in Thailand and Director (ICT)/Green Programme Office of IE Singapore, an agency under Singapore Ministry of Trade and Industry. With more than 20 years of public policy and international investment, Lena s expertise has been tapped for developing key Nation-wide masterplan and G-to-G collaboration platforms. These include Singapore-Thailand Enhanced Economic Relationship (STEER), Singapore-Tianjin Eco-city FDI Promotion, Singapore-Guangzhou Knowledge City FDI Promotion, Singapore-World Bank Infrastructure Collaboration, APEC E-Commerce Taskforce and Singapore Logistics Masterplan. Lena is an experienced speaker and judge with past participation in many international forums such as World Economic Forum, World Bank Conference, Asia Development Bank Infrastructure Forum, Asia Industrial Estates Forum, Mobile World Congress (Barcelona). Lena started her career with leading international firms such as Standard Chartered Bank, British Telecoms Services (Asia Pacific) and Creative Labs (United States/Asia). Lena holds a Bachelor of Business Administration (Honors) from National University of Singapore. She has also completed her Executive MBA, Lean Six Sigma Black Belt, Harvard Global Leadership Programme and Oxford Said Transformational Leadership Programme. She speaks fluent English, Mandarin, Thai and basic Korean. 79 The 44 th Congress on Science and Technology of Thailand (STT 44)

86 SP2_INV004 Monthian Buntan Member of the National Legislative Assembly United Nations Committee on the Rights of Persons with Disabilities SURVIVING AND MANAGING CHANGES IN THIS DISRUPTIVE ERA: HOW ABOUT THAILAND? Mr. Monthian Buntan has served as a Senator of the Upper House of the Royal Thai Parliament during 2008 to His current position is Member of the National Legislative Assembly, Royal Thai Parliament, and Member of the UN Committee on the Rights of Persons with Disabilities. He has been blind since birth. Senator Monthian earned his Master s degree from the University of Minnesota. After serving as a university lecturer for eight years, he left his stable teaching career to become a full time social activist in He has served in a number of positions within the organized blind/disability movement in Thailand, such as President of Thailand Association of the Blind for 8 years and currently serving as President of Disabilities Thailand (national umbrella organization of organizations of and for persons with disabilities). His role in the World Blind Union (WBU) began officially in 1996 as one of the blind youth committee members before he was elected to serve as a WBU executive committee member in 2000 until 2016 and now holding its honorary life membership status. Senator Monthian is proud to be a part of several major contributions: the World Summit on Information Society (WSIS) from which the first disability-inclusive policy documents in the mainstream society at the international level were created, the United Nations Convention on the Rights of Persons with Disabilities (UNCRPD), which is the first thematic international human rights law for persons with disabilities and the first international human rights treaty of the twenty-first century, and most recently, the Third World Conference on Disaster Risk Reduction (Sendai Conference) which is so far the most accessible and most disability-inclusive conference outside disability field. Senator Monthian strives to achieve Digital Inclusion through making knowledge, information and communication technologies and services inclusive of and accessible to all, including persons with disabilities. Senator Monthian has received several honors and awards, including an honorary doctorate in Social Science from Chiang Mai University where he earned his first bachelor degree, and most recently, Alumni Achievement Award 2017 from St. Olaf College in Minnesota, USA where he earned his second bachelor degree in His personal slogan is "I've given up on giving up. 80 Science and Technology in the Disruptive Era

87 SP2_INV005 Varin Sachdev News Anchor, TV & Radio Host SURVIVING AND MANAGING CHANGES IN THIS DISRUPTIVE ERA: HOW ABOUT THAILAND? Mr. Varin Sachdev has been working as a professional trilingual (Thai, English and Hindi) broadcast journalist and MC since He currently freelances as a news anchor and moderator for TNN 24, FM 101, Travel Channel and Radio Thailand (FM 88). Varin also hosts and narrates the Oscar and other Award Ceremonies live on TV and at special events. Inspired by The Amazing Race, another show he narrates live for Thai audience for the past 28 seasons. He launched his own travel and lifestyles show in 2009, The Nomad, currently airing on TNN 24 and Travel Channel. Varin has a bachelor degree in Economics from Thammasat University, MBA in Finance from Chulalongkorn University and another MA in Television and New Media, as a Fulbright scholar, from Emerson College, Boston, USA. 81 The 44 th Congress on Science and Technology of Thailand (STT 44)

88 SP3_INV001 Pichaet Wiriyachitra Asian Phytoceuticals Public Company Limited. INNOVATIVE IMMUNOTHERAPHY OF HIV/AIDS AND CANCER WITH EDIBLE PLANT EXTRACTS Operation BIM (Balancing Immunity) Research Project was established with the objective of achieving a new dimension of health care utilizing refined extracts from mangosteen, sesame, soy, guava and centella to improve the function of white blood cells. One of the Operation BIM formulations APCOcap was found to stimulate body immunity by increasing Th17 conspicuously and Th1 significantly. Clinical studies in HIV infected volunteers confirmed that the formulation considerably boosted the CD4 count, reduced the side effects from antiviral drugs and markedly improved the life quality of HIV /AIDS infected patients. The formulation reduced the risk of stage 3 AIDS patients from opportunistic infections by boosting their CD4 number to over 200 cell/cu.mm. in 1-2 months. Final stage AIDS patients with CD4 counts less than 10 cells/cu.mm. were able to raise their CD4 to an average of 500 cells/cu.mm. in 6 months. A number of patients with opportunistic infections were able to remedy their symptoms within one year and have been living normally with the undetectable viral load (less than 20 copies/cu.mm.). In summary, APCOcap boosts CD4 count rapidly and can save lives of HIV/AIDS infected patients. A similar formulation APCOcapsule was used to improve the life quality of a number of cancer patients, enable them to recover from their symptoms and live normally. The mode of action of APCOcap and APCOcapsule is mediated by increasing Th17 and Th1 which then enhance the activity of killer T cells to eliminate HIV infected CD4 cells and cancer cells. Operation BIM scientists consist of Prof.Dr.Pichaet Wiriyachitra, Assoc.Prof.Dr.Ampai Panthong, Siritip Wiriyachitra, Dr.Ganigah Ruanjahn, Prof.Dr.Souwalak Phongpaichit, Assoc.Prof.Dr.Wilawan Mahabussarakam and Assoc.Prof.Dr.Siriwan Ong-chai. APCOcap and APCOcapsule are not trademarks but are the name the researcher use in their communication. Prof.Dr. Pichaet Wiriyachitra, B.Sc.(Honours) University of Western of Australia, Ph.D. University of Tasmania, NIH Postdoctoral Fellow University of Connecticut and NSF Postdoctoral Fellow University of Pennsylvania. He served in the Prince of Sonkla University and Chiang Mai University for 26 years and published 80 scientific papers. He is the CEO of Asian Phytoceuticals Public Company Limited. 82 Science and Technology in the Disruptive Era

89 SP4_INV001 Hidehiro Uekusa Department of Chemistry, Tokyo Institute of Technology CRYSTAL STRUCTURE ENGINEERING OF PHARMACEUTICAL CRYSTAL The importance of crystalline phase of pharmaceutical solid-state cannot be overemphasized, because the active pharmaceutical ingredients (APIs) in drug are manufactured, stored, and delivered as stable crystalline phase. Therefore, knowing and characterizing the crystalline phase which is treated in each production step is essential for us. Also, the crystalline phase transforms by mechanical treatment (pressure, grinding, or granulation etc.) and heating or drying during the production steps, which causes dehydration and hydration of crystalline forms, and/or polymorphic phase transitions. The physicochemical properties of pharmaceutical crystals, i.e. solubility, stability, bioavailability, and mechanical properties etc., have strong link to the crystal structure. Thus, rational approach to deliver an effective drug with more favorable properties should be based on the structural understanding of pharmaceutical crystals, which is available through X-ray crystal structure analysis of single or powder crystals. It is usually recognized that a pharmaceutical crystal has a fixed structure and property. However, there are ways to conduct "structure engineering" (changing or altering) of the crystal in order to realize more favorable crystalline form. The polymorphic crystal and pseudo-polymorphic crystal formations or transformations between them (e.g. hydration / dehydration) offer a good opportunity of "structure engineering" the crystal to obtain new properties. These methods would be refereed as Post-crystallization engineering. Recently, co-crystal formation is focused as one of promising "Pre-crystallization engineering" methods. In the co-crystal, API and co-former molecules together make a new crystal structure and new physicochemical properties, but still it includes the same API. This "structure engineering" includes the design and synthesis of the co-crystals, and is attracting much interest in pharmaceutical and crystallographic field. In this presentation, some examples of "structure engineering" including the co-crystal formation, dehydration, and phase transition are shown, in which new physicochemical properties are developed. Examples of Post-crystallization engineering include dehydration phase transition of Acrinol (Rivanol), and erythromycin hydrates, in which the dehydrated structures were analyzed from PXRD data. "Pre-crystallization engineering" examples include valproic acid- L-arginine salt, and Benexate salt formation with artificial sweetener. Dr. Hidehiro Uekusa, Associate Professor of Tokyo Institute of Technology. He received Dr.Sci degree from Keio University in The research interests are in Organic Crystal Chemistry, and Crystallography. He awarded the scientific achievement award from Crystallographic Society of Japan in He is an associate editor of Acta Crystallographica Section C, and X-ray Structure Analysis Online journals. 83 The 44 th Congress on Science and Technology of Thailand (STT 44)

90 SP4_INV002 4 x 3 cm photo Lawrence W-Y. Wong Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong lawrwong@ust.hk CHIRAL SEGREGATION OF SPACE BY ANIONIC ASSEMBLIES FOUND IN TARTRAMIDE-BASED SPIROBORATE SALTS Recently we prepared a spiroborate anion [B(Man) 2] (Man = mandelate) for the efficient resolution of a variety of chiral cations with excess of 90%ee in the first isolation step.[1] In seeking to extend to a family of spiroborate anions with application to resolution we have found novel anionic assemblies using [B(L-TarNH 2)2], which offer prospects for the dual resolution of both cations and / or neutral solvent in the chiral layers, channels or cavities formed by their H-bonded networks.[2] In structures of around 50 salt phases studied to-date about 20 contain the sheet on the left. Inter-amide NH---O=C R 2 2(8) synthons are found to direct much of the self-aggregation, which may encapsulate cations and solvent in regular channels with chiral walls, such as the example shown on the right with R- NH 3CHMePh cations. A surprisingly wide array of structure types and nets can be found which are dependent on counter cation, solvent and crystallization conditions such as temperature and time. We are grateful to the Research Grants Council of Hong Kong for funding of this work (grant ). I graduated my PhD in Chemistry at HKUST in 2014 under Prof. Ian D. Williams. I worked as lab manager in his lab until now. My research interest focuses on crystal engineering of chiral organic crystals, pharmaceutical co-crystals and metal organic framework crystals. Five of my work were published and widely recognized with one of them features as cover article in Chem. Commun. I was selected as speaker in various conferences and awardee of rising star award in AsCA 2016 in Vietnam. 84 Science and Technology in the Disruptive Era

91 SP4_INV003 Edward R. T. Tiekink Research Centre for Crystalline Materials, Faculty of Science and Technology, Sunway University, No.5 Jalan Universiti, Bandar Sunway,Selangor Darul Ehsan, Malaysia KRYPTORACEMATES! It is well understood that under normal circumstances, during recrystallisation racemic compounds will crystallise in a centrosymmetric space group, again, usually about a crystallographic centre of inversion. A direct consequence of this is that an odd-shaped molecule will self-assemble to form, by definition, a more spherically-shaped object, which, by definition, will pack more efficiently. In the same way, molecules with chiral centres should crystallise in space groups that can accommodate enantiometrically pure molecules, that is, non-centrosymmetric space groups. Alternatively, but less commonly, racemic compounds may undergo a process termed spontaneous resolution whereby equal numbers of crystals containing one form or the other crystallise from solution, in the same non-centrosymmetric space group, forming a conglomerate, that is, a physical mixture of crystals of each hand. Even more unusually, and the focus of this presentation, are circumstances when both enantiomers co-crystallise but in a non-centrosymmetric space group. This phenomenon is termed kryptoracemic behaviour, and is quite rare in the organic solid-state as well as in structures of coordination compounds, occurring in considerably less than 1% of all crystals. The concept will be discussed in the context of recent work conducted on salts of the anti-malarial agent, mefloquine. 85 The 44 th Congress on Science and Technology of Thailand (STT 44)

92 SP4_INV004 4 x 3 cm photo Sareeya Bureekaew Faculty of School of Energy Science and Engineering Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand Sareeya.b@vistec.ac.th COMPUTATIONAL STRUCTURE PREDICTION OF (4,4)-CONNECTED COPPER PADDLE-WHEEL-BASED MOFS: INFLUENCE OF LIGAND FUNCTIONALIZATION ON THE TOPOLOGICAL PREFERENCE Metal-organic frameworks (MOFs) are crystalline porous materials constructed from metal ions and organic linkers. The framework architectures, which strongly affect to the porosity attributes, are strongly relevant to shapes of the linkers and coordination geometries around the metal ions. Describing structures of MOFs is often confusing due to the complexity of the connections between metal ions and linkers. However, the difficulty can be alleviated by using network topology which is useful for studying as well as designing MOFs. MOFs are well known for reticular chemistry. Based on the isoreticular principle, diverse linkers were used to synthesize MOFs under the same framework topology. This principle has been used to systematically generate series of MOFs with varying pore sizes and to tune other properties. However, in some special cases, this isoreticular principle is not applicable. Herein, theoretical investigation, using the forcefield MOF-FF, was employed to predict and explain the exception of isoreticular principle for a series of 4,4 net MOFs constructed from Cu paddlewheel building unit and tetracarboxylate linkers. References: 1. S. Impeng, R. Cedeno, J.P. Dürholt, R. Schmid, S. Bureekaew, Cryst. Growth Des., 2018, 18 (5), S. Bureekaew, V. Balwani, S. Amirjalayer, R. Schmid, CrystEngComm, 2015, 17 (2), S Bureekaew, S Amirjalayer, M Tafipolsky, C Spickermann, TK Roy, R. Schmid, Physica Status Solidi (b), 2013, 250 (6), Dr. Sareeya Bureekaew received a BS from the Chiangmai University (2003) and Mater degree (2006) and Ph.D (2009) in Kyoto Univeristy). She spent 3.5 years for postdoctoral work in forcefield parameterization at Ruhr-University (Germany) and 2 years in Nunyang University (Singapore). In 2015, she joined Vidyasirimedhi Institute of Science and Technology (VISTEC) as a lecturer. 86 Science and Technology in the Disruptive Era

93 SP4_INV005 James R. Ketudat-Cairns School of Chemistry, Institute of Science, & Center for Biomolecular Structure, Function and Application, Suranaree University of Technology BETA-GLUCOSIDASE CRYSTAL STRUCTURES FROM PLANTS AND BACTERIA AND THEIR USE IN MODELING HUMAN DISEASE Approximately 17 years ago, we started to investigate the structure of rice betaglucosidases and their complexes with substrates and inhibitors by x-ray crystallography as a means to understanding substrate specificity in the diverse glycoside hydrolase (GH) family 1 (GH1) enzymes found in plants. We have since solved structures for 5 GH1 enzymes in a large number of complexes. We found that the mechanisms by which these enzyme recognize oligosaccharides and differentiate substrates is rather subtle. In terms of enzymes that hydrolyze both mannosides and glucosides, combining x-ray crystallography of the complexes of Os7BGlu26 -mannosidase in complexes with transition-state mimics with QMMM simulation of the reaction revealed that the ability to bend the pyranose ring of the nonreducing sugar into two different preferred transition states appears critical for this ability. Moreover, the structure of Os3BGlu7 b-glucosidase in complex with oligosaccharides was used to explain the evolution of human Klotho family fibroblast growth factor coreceptors from sugar-binding to peptide binding proteins. During our investigation of - glucosidases, we found that there was no experimental structural model for the GH family including human GBA2 glucosylceramidase, GH116. Therefore, we produced a thermophilic bacterial -glucosidase from Thermoanaerobacterium xylanolyticum, TxGH116, and solved the structure of this protein by X-ray crystallography. The structure provides a template for modeling the human GBA2 and explaining the defects caused by disease-causing mutations in this enzyme. This basic research into the structures of plant and bacterial enzymes has provided information to design strategies to treat human disease. James R. Ketudat-Cairns received his B.Sc. from the Univ. of Puget Sound and Ph.D. from the Univ. of California, San Diego, in Biology. After a brief postdoctoral stint in the Dept. of Chemistry, UCSD, he moved to Suranaree University of Technology, where he has achieved the level of Professor and currently serves as Head of the School of Chemistry. He received the Jisnuson Svasti Protein Scientist of Thailand (PST) Award in 2017 for his efforts helping to develop protein structural biology in Thailand. 87 The 44 th Congress on Science and Technology of Thailand (STT 44)

94 SP4_INV006 Catleya Rojviriya Synchrotron Light Research Institute (Public Organization), Nakhon-Ratchasima THAILAND SYNCHROTRON RADIATION X-RAY MICROTOMOGRAPHY: FROM CLINIC TO RESEARCH APPLICATIONS X-ray microtomography (as known as micro-ct) has emerged as a non-destructive technique for visualizing internal details in three-dimensional of opaque samples. Developed from the clinical CT or CAT scan that is widely used in hospital, X-ray micro-ct optimized for research can reach microscopic details and even brings exceptionally higher resolution than CAT scan when using synchrotron radiation light source. A new X-ray tomographic microscopy (XTM) beamline has been successfully installed at SLRI. This facility is dedicated to medical and material researches that requires 3D-visualization of microscopic details inside samples such as porosity in bones and rocks, phase distribution in polymers, and defect engineering. Recent development has showed that the optical performance of XTM beamline can achieve the smallest pixel size of 1.5 microns. The CT reconstructed slices can be obtained with rapid data collection and 1000-time higher resolution than clinical CAT/CT scan. The XTM beamline has been open to external users and experiments including material, medical, and earth science. At the moment, beamline is available in polychromatic beam mode (up to 20 kev), which enables microscopic analysis such as porosity in bones and rocks, phase distribution in polymers, and defect engineering. Monochromatic beam mode will be integrated in the beamline next year. Catleya Rojviriya has her background in protein crystallography. She graduated her PhD in Biochemistry from Mahidol University. After graduation, she has worked as a post-doctoral researcher at Pohang Accelerator Laboratory, Synchrotron of South Korea for two years. In 2015, she decided to take a challenge in her life as she was offered a beamline scientist position at SLRI. She has successfully developed a new X-ray tomographic microscopy (XTM) beamline. Currently, Catleya is the beamline manager for XTM beamline, SLRI. 88 Science and Technology in the Disruptive Era

95 POSTER SESSION 89 The 44 th Congress on Science and Technology of Thailand (STT 44)

96 INFORMATION FOR POSTER PRESENTATION Time for poster attachment & location: The location for poster attachment will be specified according to Abstract ID (e.g. A1_099_PA, C2_070_PF) at the congress venue (Room GH201). The Abstract ID can be found in the Program Book. Time for poster attachment is as follows: Session Date Time POSTER ATTACHMENT Monday 29 th October :00-17:00 (GH201) POSTER REMOVAL Wednesday 31 st October :00-15:00 (GH201) REQUEST FOR ATTENDANT CERTIFICATE Monday 29 th October 2018 Tuesday 30 th October :00-16:00 09:00-16:00 (REGISTRATION DESK) DISTRIBUTION OF ATTENDANT CERTIFICATE (REGISTRATION DESK) Wednesday 31 st October :00-16:00 Poster sessions: Participants are expected to be at their posters according to the date and time assigned. Session Date Time POSTER SESSION Tuesday 30 th October :30-13:30 15:30-17:30 Wednesday 31 st October :30-13:30 90 Science and Technology in the Disruptive Era

97 SESSION A: AGRICULTURAL SCIENCE / BIOTECHNOLOGY ID Title Presenter A_001_PF COMBINED TOXICITY OF IMIDACLOPRID AND CADMIUM ON ACETYLCHOLINESTERASE ACTIVITY IN AQUATIC Pakorn Lekvongphiboon OLIGOCHAETES (TUBIFEX TUBIFEX MÜLLER, 1774) A_003_PF GROWTH AND SURVIVAL RATES OF PERINERIS. Sucharat Suksai QUATREFAGESI, (GRUBE, 1878) IN RESPONSE TO TEMPERATURES A_004_PA A MORPHOLOGICAL STUDY OF SPANISH MOSS (Tillandsia Charunee Meechoui usneoides L.) A_005_PF ALTERATION IN CATALASE ACTIVITY OF AQUATIC Panyawut Rattanarom OLIGOCHAETE, TUBIFEX TUBIFEX (MÜLLER, 1774), AFTER EXPOSED TO MIXTURE OF IMIDACLOPRID AND GLYPHOSATE A_006_PA ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE AGAINST Anumart Buakeaw CANCER CELL LINE FROM ACACIA PENNATA LEAVES EXTRACT A_007_PA PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST C- Songchan Puthong REACTIVE PROTEIN (CRP) A_008_PA DEVELOPMENT OF TEST STRIP FOR DETECTION OF Umaporn Pimpitak AFLATOXIN M1 IN MILK PRODUCTS A_009_PA VALUE ADDED OF EDIBLE ALGAE, CLADOPHORA Kangsadan Boonprab GLOMERATA (SARAI-KAI) FOR FIBER AND PROTEIN SOURCE OF YOGURT PRODUCT A_010_PF EXTRACTION OF SOLUBLE PROTEIN FROM DEFATTED RICE BRAN PLUS RICE MALT Yupakanit Puangwerakul A_011_PA SUPPRESSION OF APOPTOSIS BY YANANG (Tilliacora Boonyarit Kukaew triandra) IN METHOMYL STIMULATED RAW MACROPHAGE A_012_PA DETERMINATION OF THE CYTOTOXICITY AND ANTIOXIDANT Prakaymars Panumars ACTIVITY IN WILD EDIBLE MUSHROOM A_013_PA CARYOPHYLLENE OXIDE EXTRACT FROM OCIMUM STRAW Thanaporn Wichai TRUNK A_014_PA EFFECT OF OCIMUM SEED POWER TOWARD OIL Sajee Noitang ADSORPTION AND CHANGE OF FOOD COLORING A_018_PA THE FEASIBILITY OF USING SPENT COCONUT WATER AS Vatcharin Suwannit SUPPLEMENT FOR BACTERIAL CELLULOSE PRODUCTION A_020_PF CORRELATION BETWEEN DNJ CONTENT AND MLX56 GENE Onpawee Sangsai EXPRESSION IN MULBERRY LEAF A_030_PF POTENTIAL OF NATIVE AZOTOBACTER SPP. FROM RHIZOSPHERE ON ANTIFUNGAL ACTIVITY AGAINST Fusarium oxysporum IN LABORATORY Marisa De Bels SESSION B: BIOLOGICAL SCIENCE ID Title Presenter B1_003_PF ANTIOXIDANT ACTIVITY AND CYTOTOXICITY EFFECT OF Jongrak Attarat MUSHROOM EXTRACTS B1_004_PF EFFECTS OF SOLVENTS AND PEANUT SKIN ON ANTIOXIDANT CAPACITY AND PHENOLIC CONTENT OF RICE BRAN OILS Prasan Swatsitang 91 The 44 th Congress on Science and Technology of Thailand (STT 44)

98 ID Title Presenter B1_005_PA ANTIOXIDANT ACTIVITY OF CATIONIC AMPHIPATHIC Nisachon Jangpromma PEPTIDE MODIFIED FROM SIAMESE CROCODILE HEMOGLOBIN B1_006_PF OVEREXPRESSION AND PURIFICATION OF FICOLIN IN THE Prawit Oangkhana BLACK TIGER SHRIMP, Penaeus monodom B1_007_PA EFFECT OF CHEMICALS CROSS-LINKED IN CROCODILE Kanchana Rotcharoen (Crocodylus siamensis) AND HUMAN HEMOGLOBIN B1_008_PF DEVELOPMENT OF CANCER CELL-SPECIFIC DNA NANOBIOSENSORS Chanida Wootthichairangsan B1_009_PF ANTICANCER ACTIVITY OF ACTIVE COMPOUNDS FROM THAI MEDICINAL PLANTS ON A HUMAN NASOPHARYNGEAL Rattanavinan Hanchaina CARCINOMA CELL LINE B1_011_PA LIPASE CATALYZED ESTERIFICATION OF 3,4,5- TRIHYDROXYCINNAMIC ACID TO INCREASE ITS BIOLOGICAL ACTIVITIES Pirom Chenprakhon B1_012_PA B1_013_PA B1_014_PA B1_015_PA B1_016_PA B1_017_PF B1_018_PA B1_019_PF B2_001_PF B2_002_PA B2_003_PA B2_004_PA IDENTIFICATION OF MYB TRANSCRIPTION FACTORS CONTROLLING THE BIOSYNTHESIS OF ISOFLAVONOIDS IN Pueraria mirifica INVESTIGATION THE THERMOSTABILITY OF AMYLOMALTASE FROM Streptococcus agalactiae BY SITE- DIRECTED MUTAGENESIS AT C447 IDENTIFICATION OF DAMAGE-ASSOCIATED MOLECULAR PATTERNS (DAMPs) FROM SHRIMP Litopenaeus vannamei PHYSICAL CHROMOSOME MAP USING A PRIMED IN SITU HYBRIDIZATION TECHNIQUE FOR CULTIVATED STRAWBERRY (Fragaria ananassa) IDENTIFICATION OF C- and O-GLYCOSYLTRANSFERASES INVOLVED IN PHYTOESTROGEN BIOSYNTHESIS IN Pueraria mirifica CHARACTERIZATION OF MELATONIN-NOTCH SIGNALING AXIS IN NEUROGENESIS OF HUMAN PLURIPOTENT STEM CELLS FUNCTIONAL CHARACTERIZATION OF VAGO5 GENE IN THE LITOPENAEUS VANNAMEI IMMUNE SYSTEM UPON VIBRIO PARAHAEMOLYTICUS AHPND INFECTION ESTABLISHMENT OF STABLE INTEGRIN Β4 (ITGB4) CONDITIONAL KNOCKDOWN CHOLANGIOCARCINOMA CELL LINE THE EFFICACY OF HYDROGEN PEROXIDE, ETHYL ALCOHOL AND BIOACTIVE COMPOUNDS PRODUCED FROM Streptomyces sp. KB1 TISTR2304 AS EFFECTIVE DISINFECTANTS AGAINST MICROORGANISMS ANTIMICROBIAL EFFECT OF NOVEL SYNTHETIC PEPTIDE DESIGNED FROM Crocodylus siamensis HEMOGLOBIN HYDROLYSATE ON Escherichia coli PLASMA FROM CROCODILE (Crocodylus siamensis) REVEALING ANTIMICROBIAL ACTIVITY AGAINST Pseudomonas aeruginosa SCREENING AND IDENTIFICATION OF LIPOLYTIC PRODUCING Bacillus AND Staphylococcus STRAINS FROM SHRIMP PASTE (KA-PI) 92 Science and Technology in the Disruptive Era Kittiya Tantisuwanichkul Suthipapun Tumhom Supitcha Wanvimonsuk Kornlawat Tantivit Nithiwat Suntichaikamolkul Nut Phueakphud Hafeeza Sakor Hnin Htet Kyaw Monthon Lertcanawanichakul Sirinthip Sosiangdi Thanawan Srilert Rungsima Daroonpunt

99 ID Title Presenter B2_005_PF DECREASED EXPRESSION OF BID MOLECULE IN Jiaranai Peantum HEPATITIS B VIRUS INFECTED-LIVER CANCER CELLS AND ITS INTERACTION WITH ATG16L1 B2_006_PA PRODUCTION OF AN OPTICALLY PURE D-LACTIC ACID BY Jirabhorn Piluk Sporolactobacillus inulinus BK65-3 B2_009_PF SEROPREVALENCE OF MELIOIDOSIS IN KHON KAEN May Soe Thwe PROVINCE AND ITS ASSOCIATION WITH Opisthorchis viverrini B2_010_PA MOLECULAR CHARACTERIZATION OF EXTENDED May Thet Paing Phoo SPECTRUM β-lactamase- PRODUCING Escherichia coli IN COMPANION ANIMALS B2_012_PF SCREENING, PRODUCTION AND CHARACTERIZATION OF A Nianrawan Meecharoen HYDROPHOBIN PROTEIN FROM WHITE ROT FUNGI B2_013_PA FUNCTIONAL CHARACTERIZATION OF RESPONSIVE EFFLUX TRANSPORTATION OF ANTIBACTERIAL Tunyalux Huangsuwannakorn COMPOUNDS IN Pseudomonas aeruginosa B2_014_PF CHARACTERIZATION OF EXTENDED SPECTRUM BETA- Chanitnart Phaothong LACTAMASE-PRODUCING Escherichia coli ISOLATED FROM PATIENTS IN MEDICINE WARD, SONGKLANAGARIND HOSPITAL B2_015_PA FUNCTIONAL CHARACTERIZATION OF RBN1 AND RBN2 IN Veerakit Vanitshavit Stenotrophomonas maltophilia B2_017_PA APPLICATION OF BIO-BASED WASHING FORMULATION Rattiya Padungpol AND MIXED BACTERIA FOR REMEDIATION OF PETROLEUM- CONTAMINATED SOIL B2_018_PF THE INFLUENCE OF THE SOIL WATERING AND THALLUS Mongkol Phaengphech WETTING ON GROWTH, PHOTOSYNTHESIS AND SECONDARY METABOLITES OF THE TRANSPLANTED LICHEN Parmotrema tinctorum (DESPR. EX NYL.) Hale B2_019_PA IDENTIFICATION AND CHARACTERIZATION OF LIPASE Supalurk Yiamsombut FROM Virgibacillus halodenitrificans SKP5-4 B2_020_PA A NOVEL ACTINOPLANES ISOLATED FROM LICHEN AND ITS ANTIMICROBIAL ACTIVITY Patcharin Saeng-in B2_021_PA B3_001_PF B3_002_PF B3_003_PF B3_004_PF B3_006_PA BIODEGRADAION OF CRUDE OIL BY INDIGENOUS BACTERIAL CONSORTIUM ISOLATED FROM GULF OF THAILAND S SEDIMENT UNDER VARIOUS ENVIRONMENTAL CONDITIONS PATTERNS OF SPECIFIC IGE BOUND WHEAT PROTEINS OF INDIVIDUALS SUFFERED WHEAT INDUCED ANAPHYLAXIS MOLECULAR CLONING AND FUNCTIONAL STUDY OF Penaeus monodon RAB11-FIP2 CHARACTERIZATION OF CRUSTIN RESPONDED TO Vibrio parahaemolyticus AHPND INFECTION IN Litopenaeus vannamei CLONING AND EXPRESSION OF A HUMAN HISTONE DEACETYLASE 1 GENE IN Escherichia coli THE GENERATION OF STABLE, TRUNCATED FORMS OF Macrobrachium rosenbergii nodavirus VIRUS-LIKE PARTICLES (VLPS) AND THE PRODUCTION OF PDGFR- TARGETING VLPS Chanokporn Muangchinda Pisit Ubonsri Suphattha Kaeomani Chadapa Sakunwattana Nutthachai Lertamornthum Attthaboon Watthammawut 93 The 44 th Congress on Science and Technology of Thailand (STT 44)

100 ID Title Presenter B3_007_PF FUNCTIONAL CHARACTERIZATION OF A PATHOGEN RESPONSIVE MICRORNA IN BLACK TIGER SHRIMP Nichaphat Kanoksinwuttipong Penaeus monodon B3_008_PA CLONING, OVEREXPRESSION, AND PURIFICATION OF A Nonnanat Yooyuen GENE OF UNKNOWN FUNCTION OF PROPHAGE LOCI FROM Candidatus Liberibacter asiaticus, A CAUSAL AGENT OF HUANGLONGBING DISEASE IN CITRUS PLANTS B3_009_PA WHITE SPOT SYNDROME VIRUS ANNEXES PMO-MIR-315, Phattarunda Jaree SHRIMP MIRNA, TO REGULATE PROPHENOLOXIDASE SYSTEM VIA PPAE3 GENE SUPPRESSION B4_002_PF MONOPHOSPHORYL LIPID A (MPLA) ENHANCES INNATE Asma Longkunan IMMUNE RESPONSE TO Pythium insidiosum VACCINE B4_003_PF A THAI CASE OF DE NOVO 18QTER DELETION SYNDROME Thavorn Supaprom WITH MULTIPLE CONGENITAL DEFORMATIONS AND 46,XY,DEL(18QTER) KARYOTYPE B4_004_PF EFFECTS OF HELIUM/OXYGEN PLASMA JET ON THE SHEAR Pranisa Emyoo BOND STRENGTH OF SELF-ADHESIVE RESIN CEMENT TO DENTIN B4_005_PF SEROPREVALENCE OF LEPTOSPIRA AT COMMUNITY Chan Dara LEVEL IN NORTHEAST THAILAND B4_006_PF DEVELOPMENT OF A HEALTH CHECKUP DATA WAREHOUSE FOR COMMUNITY HEALTH DATA ANALYTICS May Soe Thu B4_007_PA B5_001_PF B5_002_PF B5_003_PA B5_004_PF B5_005_PF B5_006_PF B5_007_PF B5_008_PF B5_009_PF CONTRADICTING ROLES OF ERK1/2 IN TUMOR PROMOTING AND TUMOR SUPPRESSING ACTIVITIES OF TGF-BETA IN CHOLANGIOCARCINOMA GROWTH AND SURVIVAL OF THE EPIPHYTIC CYANOLICHEN Coccocarpia palmicola IN A TROPICAL RAIN FOREST IN THAILAND EFFECT OF LOW LIGHT ON GROWTH AND PHOTOSYNTHESIS OF Ceratophyllum demersum IN SONGKHLA LAKE PRE-ADAPTED AGGRESSIVENESS AND OPPORTUNISM OF AN INVASIVE HOUSE GECKO, HEMIDACTYLUS FRENATUS THE POTENTIAL FOR CORAL RECOVERY BY THE CORAL RECRUITMENT PATTERNS IN MU KO CHUMPHON, THE WESTERN GULF OF THAILAND ABUNDANCE OF THE WEDGE CLAM, Donax scortum FROM SMALL SCALE FISHERIES IN KRABI, TRANG AND SATUN PROVINCES CORAL RECRUITMENT PATTERNS ON SETTLEMENT PANELS IN MU KO CHUMPHON, THE WESTERN GULF OF THAILAND CORAL HEALTH AND COMMUNITY STRUCTURE ON THE SHALLOW REEF FLAT AT KO MATTRA, MU KO CHUMPHON, THE WESTERN OF GULF OF THAILAND MICROPLASTICS IN THE WEDGE CLAM Donax scortum FROM HAT PAK MENG, TRANG PROVINCE EFFECT OF NOISE ON OCCURRENCE OF BIRDS IN PUBLIC PARKS, BANGKOK Tuangporn Suthiphongchai Pitakchai Fuangkeaw Pacharee Kaewchana Yingyod Lapwong Juthamart Putthayakool Siriluck Rongprakhon Wanlaya Klinthong Sittiporn Pengsakun Laongdow Jungrak Panisa Aimvijarn 94 Science and Technology in the Disruptive Era

101 ID Title Presenter B5_010_PF GONADAL DEVELOPMENT OF DIFFERENT SIZE CLASSES Charernmee Chamchoy OF THE WEDGE CLAM Donax scortum IN TRANG PROVINCE, THE ANDAMAN SEA B5_011_PA BIRD DIVERSITY IN CHULABHORN DAM, CHAIYAPHUM Sarun Keithmaleesatti PROVINCE B5_012_PF WHITE SYNDROME IN SCLERACTINIAN CORALS FROM KO SAMSAO, MU KO ANGTHONG, THE WESTERN GULF OF THAILAND Wiphawan Aunkhongthong B5_013_PF REEF FISH COMMUNITY COMPOSITION IN MU KO CHUMPHON AND MU KO ANG THONG NATIONAL PARKS, WESTERN GULF OF THAILAND Felipe Monteiro Gomes de Mattos B5_014_PF B5_015_PF B5_017_PF POTENTIAL OF ECOTOURISM DEVELOPMENT AT SOME CORAL REEFS AND UNDERWATER PINNACLES IN CHUMPHON PROVINCE TOURISM CARRYING CAPACITY ASSESSMENT AT SOME DIVE SITES IN CHUMPHON PROVINCE BIODIVERSITY OF DISCOCRUSTOSE LICHEN OF MANGROVE FOREST IN THE EASTERN COAST OF THAILAND. Makamas Sutthacheep Thamasak Yeemin Phimpisa Phraphuchamnong SESSION C: CHEMISTRY ID Title Presenter C1_001_PF A NOVEL OPTICAL SENSOR FOR DETERMINING NITRATE IONS IN WATER USING POLYVINYLPYRROLIDONE AND Cholapat Varongchayakul ACID BLUE 22 C1_002_PA SIMULTANEOUS DETERMINATION OF TRACE LEAD AND Saowapa Planonth ANTIMONY IN GUNSHOT RESIDUES BY VOLTAMMETRY C1_003_PA DETERMINATION OF CYANIDE IN CONCRETE ROOFING TILES BY DIFFERENTIAL PULSE VOLTAMMETRIC METHOD Jaroon Junsomboon C1_004_PA C1_005_PA C1_006_PF C1_008_PA C1_009_PF STUDY OF THE REMOVAL EFFICIENCY ON METHYLENE BLUE UNDER VISIBLE LIGHT BY USING Cu2O PRECIPITATION WITH ZnO CATALYST IN PHOTOCATALYSIS METHOD DEVELOPMENT TEST KITS FOR DETERMINATION BLOOD IN EVIDENCE AT A CRIME SCENE SIMPLE AND RAPID MEASUREMENT OF ETHANOL IN PERFUME BY PORTABLE RAMAN SCATTERING SPECTROMETER: POTENTIAL PERFUME ETHANOL POISON FOR INFANTS POLYPYRROLE-DOPED CARBON BLACK MODIFIED ON SCREEN-PRINTED CARBON ELECTRODE FOR GENISTEIN DETECTION USING IONIC LIQUID AS SUPPORTING ELECTROLYTE ELECTROCHEMICAL DETERMINATION OF GOLD IONS IN GOLD PLATING SOULUTION USING PAPER-BASED SCREEN-PRINTED GRAPHENE ELECTRODE Patchariya Chawalitjinda Udomsak Palee Nopchonsorn Manpiankit Surinya Traipop Phichanan Duchda C1_011_PF MODIFICATION OF BENTONITE FOR DYE ADSORPTION Benjamaporn Jutapad 95 The 44 th Congress on Science and Technology of Thailand (STT 44)

102 ID Title Presenter C1_012_PA ELECTROCHEMICAL SENSOR OF CO2 BASED ON SURFACE Orrapa Cheycharoen MODIFIED HALLOYSITE NANOTUBE C2_003_PA SELECTIVE NAKED-EYE SENSING FOR OXALATE USING Nattawat Chatphueak DUNUCLEAR COPERR(II) COMPLEX UNDER INDICATOR DISPLCEMENT ASSAY C2_004_PF THE ROLE OF THE SIZE OF HYDROXYAPATITE Natthaya Thepphanao NANOPARTICLE ON OSTEOBLAST CELL TOXICITY AND THEIR APPLICATION AS MIRNA CARRIER SYSTEM C2_005_PF SYNTHESIS AND STABILITY STUDY ON ZnONPs WITH Poonyawee Keattanong DIMERCAPTOSUCCINIC ACID C3_004_PF RELATIVE KINETIC REACTIVITY OF ALKENES, ALLENES, Jaturavit Nimnuan AND ALKYNES IN THERMAL DIELS ALDER REACTIONS C3_005_PF SYNTHESIS AND CYTOTOXICITY OF HALOGENATED Dinh Phuoc Nguyen CHALCONE-BASED PYRAZOLINE DERIVATIVES C3_006_PF SYNTHESIS AND CYTOTOXICITY OF MANSONONE G Nhung Ngo Thi Tuyet DERIVATIVES C3_007_PF NITROAROMATIC SENSORS FROM PYRENYL QUINOLINE Chantipa Lerswipapat DERIVATIVES C3_008_PA INVESTIGATION OF ANTI-INFLAMMATION PROPERTIES IN Mary Sriwisade PIPERAZINE-2,5-DIONE DERIVATIVES C3_009_PA MEROTERPENOIDS FROM THE LEAVES OF Psidium guajava Parichat Thepthong C3_010_PA ONE-POT SYNTHESIS OF 2,3-UNSATURATED-O-GLYCOSYL Rungnapha Saeeng TRIAZOLES DERIVATIVES BY FERRIC CHLORIDE AS CATALYST C3_011_PA CLERODANE DITERPENES FROM TWIG EXTRACTS OF Isaraporn Polbuppha Enicosanthum membranifolium C3_012_PA CHEMICAL CONSTITUENTS FROM THE LEAVES EXTRACTS Virayu Suthiphasilp OF Desmos dumosus C4_001_PA RAPID REMOVAL OF TETRACYCLINE ANTIBIOTICS FORM Kwanjira Phanplado AQUEOUS SOLUTION BY USING LAYERED DOUBLE HYDROXIDE COMPONENT C4_002_PF THE EFFECTS OF ZEOLITE FRAMEWORKS ON ADSORPTION Piboon Pantu THERMODYNAMIC OF 1-BUTANOL IN THETA-1 AND BETA ZEOLITES C4_003_PA DEVELOPMENT OF PAPER-BASED SENSOR WITH Foyfon Sengsong PORTABLE DEVICE FOR REAL TIME FIELD WORK DETECTION OF ARSENIC I WATER RESOURCES BY ELECTROCHEMICAL METHOD C4_005_PA THE ROLE OF Ni(111) FOR PROPYLENE PRODUCTION VIA Tinnakorn Saelee PRORPANE DEHYDROGENATION PROCESS C4_006_PA EXTENDING POLYCALICENE CHEMICAL SPACE Thawalrat Ratanadachanakin C4_008_PA STRUCTURE AND DYNAMICS OF SERINE HYDROXYMETHYLTRANSFERASE WITH PYRIDOXAL PHOSPHATE AND TETRAHYDROFOLATE BOUND BY MOLECULAR DYNAMICS SIMULATION Peerapong Wongpituk 96 Science and Technology in the Disruptive Era

103 SESSION D: POLYMER & MATERIALS SCIENCE / NANOTECHNOLOGY ID Title Presenter D_002_PF CORN STARCH FILM REINFORCED WITH CITRATE Prapaporn Nakarat CELLULOSE NANOCRYSTALS D_003_PF PREPARATION OF DNA-DENDRIMER MOLECULAR HYBRID AS CARRIER FOR DRUG DELIVERY SYSTEM Boonchoy Soontornworajit D_004_PF TEMPO-OXIDIZED BACTERIAL CELLULOSE FOR Panida Watchanung REINFORCING ALGINATE-BASED FILMS D_005_PF ACETALIZATION OF GLYCEROL USING SULFONIC ACID Ratikorn Vongkhae FUNCTIONALIZED ALUMINOSILICATE CATALYSTS UNDER SOLVENT-FREE CONDITION D_006_PF EFFECT OF MIXING TIME ON THE MECHNICAL PROPERTIES OF BIO-BASED FLEXIBLE POLYURETHANE FOAM Ruedee Jaratrotkamjorn D_007_PA GREEN SENTHESIS OF SILVER NANOPARTICLES USING Abdulnaser Hajisamoh Cassia alata LEAF EXTRACT AND THEIR CATALYTIC ACTION IN REDUCTION OF DYE D_008_PA FORMATION OF GELATIN PARTICLES VIA SCHIFF BASE REACTION IN EMULSION SYSTEM Paphitchaya Khomweerawong D_011_PF INFLUENCES OF Ca/Si RATIO ON PHASES AND Aunsaya Eksatit PROPERTIES OF HYDRATED LIME AND SILICA SAND MIXTURES UNDER HYDROTHERMAL CONDITION D_013_PA THE EFFECT OF CALCINATION TIME AND CALCINATION Tidapa Rattanaumpa TEMPERATURE ON ENHANCING HYDROPHOBICITY OF MESOPOROUS SILICA NANOPARTICLES (MSNs) D_014_PF PROPERTIES AND FRACTURE BEHAVIOR OF BAMBOO Supaphorn Thumsorn FIBER REINFORCED POLY(BUTYLENE SUCCINATE) COMPOSITES D_016_PF EFFECT OF ACID TREATMENT ON PROPERTIES OF Abdulhakim Masa NATURAL RUBBER/ EPOXIDIZED NATURAL RUBBER/HALLOYSITE NANOTUBES COMPOSITES D_017_PA FABRICATION OF NANOSTRUCTURE TIO2 BY ANODIZATION Vanida Lumpol METHOD D_018_PA CURCUMIN-MODIFIED TITANIUM DIOXIDE NANOTUBES AND THEIR PHOTOCATALYTIC PERFORMANCE Cheewita Suwanchawalit D_019_PF NATURAL EXTRACT-BASED FLAME RETARDANT COTTON Kittibandid Soisuwan FABRIC D_020_PF PREPARATION OF NANOFIBER CELLULOSE/POLY(VINYL Butsarin Thaweekoon ALCOHOL) FILMS AND THEIR PROPERTIES D_021_PF GEMOLOGICAL CHARACTERISTICS AND CHEMICAL Kanyarat Kwansirikul COMPOSITIONS OF COLOR-CHANGE GLASS BEING MARKETED AS SYNTHETIC ZULTANITE D_022_PF UTILIZATION OF AGRICULTURAL WASTE AS ABSORBENT Pimchanok Patho FOR THE REMOVAL OF PHENOL RED AND BROMOCRESOL GREEN FROM AQUEOUS SOLUTION D_023_PF EFFECTS OF VULCANIZATION SYSTEM ON PROPERTIES OF Nustsaba Kronburee SILICA REINFORCE NATURAL RUBBER COMPOSITES PREPARED FROM FRESH LATEX D_025_PF REDUCED GRAPHENE OXIDE/COBALT SULFIDE HYBRID FILM FOR PHOTOCATALYTIC DEGRADATION OF DYE Anwaraporn Suramitr 97 The 44 th Congress on Science and Technology of Thailand (STT 44)

104 ID Title Presenter D_026_PA EVALUATION OF ANTIBACTERIAL AND ANTI-FREE RADICAL ACTIVITIES OF COTTON FABRIC STAINED WITH NATURAL DYE USING CHITOSAN AS MORDANT Sucharat Sanongkiet SESSION E: ENERGY / ENVIRONMENTAL& EARTH SCIENCE ID Title Presenter E_001_PA THE DEPOSITION OF DUST FALL ON ROADSIDE IN FRONT Pajaree Thongsanit OF NARESUAN UNIVERSITY E_005_PF CHARACTERISTICS AND POSSIBILITIES OF INCINERATION Sutthida Boonsamran RESIDUES FROM BIOMASS-FIRED POWER PLANT IN ROAD CONSTRUCTION E_006_PF MATERIAL FLOW ANALYSIS FOR WASTE MANAGEMENT IN Noulkamol Arpornpong ONSHORE PETROLEUM EXPLORATION AND PRODUCTION INDUSTRY IN THAILAND E_011_PF BIODIESEL PRODUCTION FROM WASTE COOKING OIL USING CALCIUM OXIDE DERIVED FROM WASTE STARFISH AS HETEROGENEOUS CATALYST Apisit Prokaew SESSION F: PHYSICS / APPLIED PHYSICS ID Title Presenter F_002_PF HOLOGRAPHIC CONDUCTIVITY OF MAXWELL FIELD Suphot Musiri COUPLED WITH A SCALAR FIELD IN SCWARZSCHILD AdS SPACETIME F_003_PA ANALYSIS ISOCLINIC FRINGE PATTERN OF PMMA BY Yongyut Manjit USING PLANE REFLECTION POLARISCOPE F_004_PF INFLUENCE OF STOICHIOMETRIC COMPOSITION ON Montree Hankoy STRUCTURAL, OPTICAL AND ELECTRICAL PROPERTIES OF IN2S3- DOPED CDSE1-XSX THIN FILMS PREPARED BY VACUUM THERMAL EVAPORATION METHOD F_005_PF QED CORRECTION OF LOW ENERGY PHOTON-PHOTON SCATTERING CROSS SECTION Suppanat Supanyo SESSION G: MATHEMATICS / STATISTICS / COMPUTER SCIENCE ID Title Presenter G_001_PA CLASSIFICATION OF SEASONS IN EACH REGION BY FILTER Anan Sirithanyarat - FUZZY LOGIC METHOD SESSION H: FOOD SCIENCE & TECHNOLOGY ID Title Presenter H_001_PA PRODUCTION OF RICE MILK ICE CREAM REDUCED FAT FOR Narin Charoenphun HEALTH H_002_PF MODIFICATION OF EGG ALBUMEN FILM BY THE MAILLARD Pinyapat Jitphongsaikul REACTION H_003_PA THE PASTEURIZATION OF MILK AND LACTOSE-FREE MILK APPLYING OHMIC HEATING IN COMPARISON WITH CONVENTIONAL METHOD Napat Suebsiri 98 Science and Technology in the Disruptive Era

105 ID Title Presenter H_004_PF DEVELOPMENT OF KLUAI HIN SMOOTHIE AS AN ALTERNATIVE PRODUCT FOR THE LOCAL FARMER AND THE HEALTH CARE CONSUMER Patthamawadee Tongkaew H_006_PA H_007_PA H_008_PF H_009_PF H_010_PA H_011_PA H_012_PA H_013_PA H_015_PF H_016_PA H_017_PF H_019_PF H_020_PF H_021_PF MICROBIOLOGICAL SURVEY OF COLIFORMS AND Escherichia coli IN LEAFY GREENS FROM PUBLIC MARKET A REAL-TIME LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR RAPID DETECTION OF Salmonella spp. IN FOOD SAMPLES THE EFFECT OF ULTRASOUND ON MASS TRANSFER AND TEXTURE OF DRIED OSMOTIC DEHYDRATED COCONUT EFFECT OF STORAGE CONDITIONS ON SENSORY AROMA CHARACTERISTICS OF KHAO DAWK MALI-105 BROWN RICE ANTIOXIDANT ACTIVITIES OF CRUDE EXTRACTS OF EDIBLE MUSHROOM COLLECTED FROM CHIANG RAI PROVINCE PREVENTION OF BROWNING FORMATION IN JASMINE FLOWER (Jasminum sambac) DURING POSTHARVEST STORAGE EFFECT OF SEVERITY FACTOR ON SUBCRITICAL WATER EXTRACTION OF OLIGOSACCHARIDES FROM PASSION FRUIT PEEL UNRIPE GREEN BANANA FLOUR (Cavendish spp.): SOME PROPERTIES AND ITS APPLICATION OPTIMIZATION OF COCONUT WATER POWDER BY USING SPRAY DRYING AND FOAM MAT DRYING METHOD: EFFECT OF DRYING CONDITIONS ON PHYSICO-CHEMICAL AND SENSORY PROPERTIES PROPERTIES OF BANANA (Cavendish spp.) STARCH FILM INCORPORATED WITH BANANA PEEL EXTRACT AND ITS APPLICATION CHARACTERIZATION OF PROTEIN ISOLATE FROM SACHA INCHI PRESSED-CAKE BY USING THREE-PHASE PARTITIONING TECHNIQUE DEVELOPMENT OF HIGH PROTEIN SNACK PRODUCT FROM RICEBERRY RICE, SOY AND EGG EVALUATION OF SOLID-LIQUID EXTRACTION KINETICS OF ANTIOXIDANT COMPUNDS, TOTAL PHENOLS, AND MAJOR SWEETENING AGENTS FROM Stevia Rebaudiana LEAF SENSORY CHARACTERISTICS AND CONSUMER PREFERENCE MAPPING OF PORRIDGE SESSION SP4: CRYSTALLOGRAPHY Tumnoon Charaslertrangsi Narong Arunrut Saowanee Lertworasirikul Supeeraya Arsa 99 The 44 th Congress on Science and Technology of Thailand (STT 44) Suchawadee Insawang Supawadee Somkane Thaweesak Tana Orapan Romruen Parichart Yamalee Wantida Taweechat Orapan Romruen Pukchunya Siriwan Natthakan Rungraeng Aratchaporn Deeprasert ID Title Presenter SP4_001_PA TWO NOVEL LANTHANIDE COORDINATION POLYMERS BASED ON CHLOROBENZHYDRAZIDE DERIVATIVES Nucharee Chongboriboon SP4_002_PA SERIES OF ZINC(II) INTERPENETRATED COORDINATION POLYMERS BASED ON TRICARBOXYLATE AND DIFFERENT Kenika Khotchasanthong N-CONTAINING LIGANDS SP4_003_PA A NEW LUMINASCENT POROUS LANTHANIDE COORDINATION POLYMER FOR SELECTIVE SENSING OF SMALL ORGANIC MOLECULE AND METAL IONS Nutcha Ponjan

106 ID Title Presenter SP4_004_PA SINGLE CRYSTAL TO SINGLE CRYSTAL Kodchakorn Samakun TRANSFORMATIONS IN COPPER-BIPYRIDINE- TEREPHALATE FRAMEWORKS SP4_006_PA LANTHANIDE COORDINATION POLYMERS WITH BENZOATE DERIVATIVES LIGAND: THERMAL AND OPTICAL PROPERTIES Chatphorn Theppitak SP4_008_PF THE NOVEL COPPER(II) COMPLEX CONTAINING BY 2- HYDROXYBENZOIC ACID AND 2-AMINOPYRIMIDINE FOR GAS SENSOR APPLICATION SP4_010_PA IMPROVEMENT OF PRODUCTION AND CRYSTALLIZATION OF RECOMBINANT CRUSTINPm1 FROM THE BLACK TIGER SHRIMP Penaeus monodon SP4_011_PA SYNTHESIS AND STRUCTURAL CHARACTERIZATION OF THE COPPER (I) MIXED COMPLEXES WITH 5-METHYL 1,3,4 THIADIAZOLE 2-THIOL AND TRIPHENYLPHOSPHINE LIGANDS SP4_012_PA SYNTHESIS, SPECTROSCOPIC CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF TWO NEW MONONUCLEAR TERNARY NICKEL(II) COMPLEXES SP4_014_PA A LUMINESCENT ANIONIC METAL-ORGANIC FRAMEWORK FOR HIGHLY SELECTIVE Cu2+ SENSING SP4_015_PA A NOVEL THREE-DIMENTIONAL INTERPENETRATING COPPER(II) COORDINATION POLYMER CONSTRUCTING FROM 4,4 -BIPYRIDINE AND 2-HYDROXYBENZOATE LIGANDS SP4_016_PA NOVEL MOF LUMINESCENT PROBE FOR EFFICIENT SENSING OF COPPER(II) AND IRON(III) IONS SP4_017_PA SYNTHESIS, CHARACTERIZATIONS AND X-RAY STRUCTURAL STUDIES OF NOVEL ONE-DIMENSIONAL LADDER-LIKE STRUCTURE COBALT(II) COORDINATION POLYMER SP4_018_PA TWO NEW SILVER(I) COORDINATION POLYMERS BASED ON FLEXIBLE SCHIFF BASE LIGANDS SP4_019_PA CRYSTAL STRUCTURE AND HIRSHFELD SURFACE ANALYSIS OF 4,4'-(5,5'-DISULFANEDIYLBIS(TETRAZOLE- 5,1-DIYL))DIBENZOIC ACID SP4_020_PA A SERIES OF TWO-DIMENSIONAL LANTHANIDE COORDINATION POLYMERS BASED ON THE CHLORANILIC ACID SP4_021_PA A NOVEL ZINC(II) COORDINATION POLYMER (ZN(II)-CP) BASED ON MIXED-LIGANDS AS FE(III) SENSOR SP4_022_PA SYNTHESIS AND PROPERTIES OF A NEW ADENINE-BASED Zn (II) COORDINATION POLYMERS FOR SENSITIVE SENSING OF NITROAROMATICS Pornsan Lueangseephet Thatcheewa Apichatayanon Khin Su Su Han Phichitra Phiokliang Natthakorn Phadungsak Nanthawat Wannarit Kittipong Chainok Phakamat Promwit Aiyada Rungruang Napatsorn Sawengsiriphon Akkawat Udompipat Jiraporn Buasakun Phakinee Srilaoong 100 Science and Technology in the Disruptive Era

107 SESSION A: AGRICULTURAL SCIENCE / BIOTECHNOLOGY 101 The 44 th Congress on Science and Technology of Thailand (STT 44)

108 A_001_PF: COMBINED TOXICITY OF IMIDACLOPRID AND CADMIUM ON ACETYLCHOLINESTERASE ACTIVITY IN AQUATIC OLIGOCHAETES (Tubifex tubifex Müller, 1774) Pakorn Lekvongphiboon, Nalena Praphairaksit* Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10300, Thailand * Abstract: Imidacloprid is a pesticide that has contaminated water sources near agricultural areas since However, not only is there insecticide in the water but also heavy metals, especially cadmium. This research aims to evaluate the toxicity of imidacloprid and cadmium in both individual and combined cases in Tubifex tubifex, using the activity of acetylcholinesterase as a biomarker. In the individual cases, the LC50 values of imidacloprid and cadmium after 48 hours immersing in the water were and mg L - 1 respectively. When measuring the activities of the acetylcholinesterase at 0.8LC50 values, the activities were significantly higher than the untreated control groups (p<0.05). However, the LC50 of the combined case, imidacloprid and cadmium, was mg L -1 (the additive index was ), which shows that these two chemicals have a synergistic relationship. Moreover, the acetylcholinesterase activity measured at 0.8 and 0.2 of LC50 values of the combined case which were also significantly higher than the untreated control groups (p<0.05). In conclusion, the acetylcholinesterase activity can be used as an effective indicator for assessing the impacts of imidacloprid and cadmium that has been co-contaminating the freshwater ecosystem. 102 Science and Technology in the Disruptive Era

109 A_002_OF: CONSTRUCTION OF GENETIC LINKAGE MAP BASED ON SIMPLE SEQUENCE REPEAT MARKERS AND IDENTIFICATION QTL CONTROLLING ECONOMICALLY IMPORTANT TRAITS IN CASSAVA (Manihot esculenta Crantz) Todsapol Kornsri, Supajit Sraphet, Nattaya Srisawad, Duncan R Smith, Saovaros Svasti, Kanokporn Triwitayakorn* Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand * kanokporn.tri@mahidol.ac.th Abstract: In this study, informative polymorphic SSR markers were identified in an F 1 population of cassava derived from a cross between Rayong3 (female) and Piroon1 (male), then applied to construct genetic linkage map of cassava. Finally, quantitative trait loci (QTL) controlling economically important traits in cassava. Total of 876 pairs of SSR primers were identified informative polymorphic markers. The results revealed that 860 (98.17%) primer pairs were amplifiable, including 772 (88.12%) informative, 2 (0.22%) non-informative, and 86 (9.81%) monomorphic primers, whereas 16 primer pairs (1.82%) showed no PCR products. Therefore, 244 informative primers were used to genotype with 81 samples of the F 1 population. Then, genotype data were subjected to construct the genetic linkage map using the computer software package joinmap /version 3.0. The results showed genetic linkage map is composed of 63 SSR markers, covering cm (Kosambi cm), distributed on 18 linkage group with an average marker distance of cm. Finally, The QTL position were identified underlying economically important traits including main stem, apices, first branch height, second branch height, plant height and level of plant using the computer software package MapQTL /version 4.0. A total of 4 QTL were found underlying traits of apices, second branch height and level of plant. The results of this study will be useful and applicable in marker assisted selection for cassava breeding program in order to develop new cassava varities. 103 The 44 th Congress on Science and Technology of Thailand (STT 44)

110 A_003_PF: GROWTH AND SURVIVAL RATES OF Perineris quatrefagesi, (Grube, 1878) IN RESPONSE TO TEMPERATURES Sucharat Suksai, Supanut Pairohakul* Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand * Abstract: Perineris quatrefagesi is a commonly distributed polychaete species with high commercial value in Thailand. Polychaete farming are mostly utilized as an open system and located along the coast of Thailand. Therefore, these could lead to the challenges in terms of temperature that could directly affected to the animals. This study investigated the effect of temperature on growth and survival rate of P. quatrefagesi at various temperatures (27, 29, 31, 33 C). The wet weight, survival rate and specific growth rate (SGR) were observed and measured. The results showed a significant change in the final wet weight regarding the different temperature levels. While the survival rate and SGR gave the similar pattern with significantly difference at the highest temperature. The lowest growth and survival rate at the highest temperature could be explained by reducing of feeding rate and increasing in metabolism which animals could not compensate during a long time period. This study, therefore, revealed the optimal temperature for culture this species at 29 C, while 27 C and 31 C were also acceptable with regard to the high survival rate. 104 Science and Technology in the Disruptive Era

111 A_004_PA: A MORPHOLOGICAL STUDY OF SPANISH MOSS (Tillandsia usneoides L.) Charunee Meechoui 1 * 1 Agricultural Technology Research Institute. PO Box 89, Lampang 52000, Thailand * charunee.rmutl@gmail.com: Abstract: Morphological characters of Spanish moss (Tillandsia usneoides L.) were observed under microscope. Results were shown in figure 1. General appearance on the surface of leaf and stem are covered by imbricated translucent trichomes (scale). It presents anomocytic stomata in the epidermal cell. A cross section of the stem showing fiber-vascular tissue. Figure 1. Morphological features of Spanish moss (Tillandsia usneoides L.) A: habit; D: leaf and stem; B&E: portion of leaf covered with dense trichomes; C&F: structure of trichome; G: surface view of stem showing some fluorescent part of trichomes (tc); H: surface view of leaf, showing epidermal cell (ep) and stomata (st); I: cross section of stem showing fiber-vascular tissue (fv). Note: B-C photomicrograph (light microscope); D-F Schematic drawing (under light microscope); G-I photomicrograph (fluorescence microscope, UV wavelength) 105 The 44 th Congress on Science and Technology of Thailand (STT 44)

112 A_005_PF: ALTERATION IN CATALASE ACTIVITY OF AQUATIC OLIGOCHAETE, Tubifex tubifex (MÜller, 1774), AFTER EXPOSED TO MIXTURE OF IMIDACLOPRID AND GLYPHOSATE Panyawut Rattanarom, Nalena Praphairaksit* Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10300, Thailand * Abstract: Although pesticides were frequently detected as mixtures in the natural aquatic environment, most of the research has only emphasized on investigating the effects of single pesticides on non-target aquatic species. This might not reflect the existent impacts of environmental contamination. In this study, the combined toxicity of two commonly used pesticides, imidacloprid and glyphosate, were evaluated using aquatic worms, Tubifex tubifex, as a test organism. The results have shown that imidacloprid was more toxic toward T. tubifex when compared to glyphosate at the 24-h LC 50 values of mg L -1 and mg L -1. Additionally, the joint toxicity of these two pesticides at 24 hours was found to have the antagonistic effect with the 24-h LC 50 values of mg L -1. The activities of catalase with the sub-lethal concentrations were also measured in this study. The results revealed that there were significant increased (p<0.05) in the catalase activities in the treated worms compared to untreated groups. The catalase activities increased as the concentrations of the pesticides increased. Therefore, the catalase activity can be used as an effective biomarker of imidacloprid, glyphosate as well as their mixture compound at 24 hours of exposure time. Even though the mixture of imidacloprid and glyphosate exhibited antagonistic property, this interaction could have resulted in several severe sub-lethal effects. 106 Science and Technology in the Disruptive Era

113 A_006_PA: ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE AGAINST CANCER CELL LINE FROM Acacia pennata leaves extract Songchan Puthong, Kittinan Komolpis, Umaporn Pimpitak and Anumart Buakeaw* Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, Thailand * Abstract: Natural anti-oxidants from vegetables which are normally used in daily cooking are of great interest because they are safer to be used than synthetic anti-oxidant compounds. It has been reported that phytochemicals especially some phenolic compounds can act as the anti-oxidant. Among the twenty Thai indigenous vegetables studied, Cha-om (Acacia pennata) contained the highest phenolic contents at 45.3 µg gallic acid/mg (dry wt.). Therefore, this research was focused on the anti-oxidant activity of crudes extracted from both fresh (F) and dried (D) leaves of A. pennata. Leaves were extracted by either water (H) or 95% ethanol (E). Total phenolic contents of the crude extracts, FH, FE, DH and DE were quantified by Folin-Ciocalteu colorimetric method and found to be 40.17, 33.74, and µg/mg of extract, respectively. Anti-oxidant activity, based on DPPH assay, of the extracts was quantified in term of the 50% radical scavenging activity. It was found that the FH and DH extracts showed higher activity at 3.91 mg/ml and 4.13 mg/ml than the FE and DE did at 7.80 mg/ml and mg/ml. The anti-oxidant activity in term of reducing ability was also analyzed by FRAP assay. All extracts, FH, FE, DH and DE yielded slightly different abilities at 5.09, 4.10, 5.01 and 2.91 mm, respectively. In addition, anti-cancer activity of was assayed by MTT colorimetric methods. The activity was reported in term of 50% inhibition concentration, IC 50. Interestingly, the FE extract yielded the lowest IC 50 value of 1.34 µg/ml against breast cancer cell line MDA-MB The 44 th Congress on Science and Technology of Thailand (STT 44)

114 A_007_PA: PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST C-REACTIVE PROTEIN (CRP) Sirirat Rengpipat, Kittinan Komolpis, Umaporn Pimpitak, Anumart Buakeaw and Songchan Puthong* Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, Thailand * Abstract: C-reactive protein (CRP) is a protein which human body produces at a higher level in response to tissue inflammation. CRP has been linked as a biomarker for inflammation and coronary heart disease. Normally, the concentration of CRP is approximately mg/ml. But, following an acute-phase stimulus, circulating CRP concentration in blood is increased up to 100-1,000 folds. A detection method of CRP in laboratory is the immunological method based on the specific binding between CRP and its antibody. Therefore, antibody specific to only CRP is the most important factor for CRP-assay development. This research focused on the production of monoclonal antibody (mab) specific to CRP for CRP- detection development. Conventional cell fusions between splenocytes from 3 BALB/c mice and NS-I myeloma cells were performed to obtained hybridoma cells. After screening based on the ability to produce mab and specificity, 18 monoclones were selected. Among these clones, clone #8 showed high sensitivity based on an indirect enzyme linked immunosorbent assay (ELISA) with the 50% of effective concentration (EC50) lower than 3.1 ng/ml while clone #48 and #61 showed high sensitivity based on an indirect competitive ELISA with the 50% inhibition concentration (IC 50) value of 1.0 and 0.4 µ l/ml, respectively. Isotype of the three mabs was found to be IgG 2a. Epitope mapping of the three mabs indicated that mab #8 and #48 binds with CRP at the same epitope. But mab #61 binds with CRP at the different epitope of those two mabs with the absorbance index of 55. These results indicated that the obtained mabs have potential to be used in the development of CRP detection based on the immunological principle. 108 Science and Technology in the Disruptive Era

115 A_008_PA: DEVELOPMENT OF TEST STRIP FOR DETECTION OF AFLATOXIN M1 IN MILK PRODUCTS Kittinan Komolpis, 1 Sirirat Rengpipat, 2 Songchan Puthong, 1 Anumart Buakeaw, 1 and Umaporn Pimpitak 1, * 1 Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, Thailand 2 Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand * umaporn.p@chula.ac.th Abstract: Aflatoxins (AFs) are toxic substances produced by Aspergillus spp. fungi. Major AFs commonly found in feedstuffs are aflatoxin B1 (AFB1), aflatoxin B2, aflatoxin G1 and aflatoxin G1. When consumed by animals, these AFs are metabolized and partly accumulated inside the body and partly released into urine, feces and milk. Aflatoxin M1 (AFM1) is the metabolite found in milk of the animals fed with the AFB1-contaminated feedstuffs. World Health Organization (WHO) categorized AFs and AFM1 as one of the most dangerous carcinogenic substances. Unites States Food and Drug Administration (US FDA) have set the minimum residual limit (MRL) for AFM1 at 0.5 ppb (ng/ml). In Thailand, MRL of AFM1 has not been set but the MRL has been set for total aflatoxins (all AFs) at 20 ppb. Therefore, detection of AFM1 residues in milk is required to ensure safety of consumers, especially children. Generally, a detection method suitable for screening a large number of samples is based on immunological method in the format of either 96 wells plate or test strip (lateral flow immune assay). These methods require a suitable antibody which is specific to the substance of interest. The Institute of Biotechnology and Genetic Engineering (IBGE) had a success in the production of monoclonal antibody (mab) against AFM1. Limit of AFM1 detection of an enzyme linked immunosorbent assay in the 96 wells plate was found to be 0.04 ppb. In this research, the produced mab was used in the development of test strip based on a competitive principle for detection of AFM1 in milk samples. Major components of the test strip are sample pad, conjugate pad, reaction pad, adsorption pad and backing card. On the conjugate pad, anti-afm1 mab conjugated with gold nanoparticle was loaded at the amount of 0.05 µg/strip. On the reaction pad, bovine serum albumin-afm1 conjugate (0.24 µg/strip) was immobilized at the test line while goat anti-mouse antibody (0.06 µg/strip) was immobilized at the control line. The prepared test strip gave the cut-off value of AFM1 at 0.5 ppb. Purple color at the test line was not observed when tested with milk samples containing 0.7 ppb AFM1 (truly positive samples) while purple color was observed when tested with milk samples containing 0.3 ppb AFM1 (truly negative samples). In case of truly uncertain milk samples containing ppb AFM1, light intensity of purple color at the test line was observed. The developed test strip was suitable for raw milk, pasteurized milk, sterilized milk, UHT milk, powder milk (no starch). However, the test strip could not be used for milk samples flavored with chocolate and coffee due to strong color interference. 109 The 44 th Congress on Science and Technology of Thailand (STT 44)

116 A_009:PA: VALUE ADDED OF EDIBLE ALGAE, Cladophora glomerata (SARAI-KAI) FOR FIBER AND PROTEIN SOURCE OF YOGURT PRODUCT Kangsadan Boonprab,* Siripan Kerdsut,Pichaiwat Chanalardpaisarn, Sasithon Khunthong, Fishery products department, Fisheries Faculty, Kasetsart University, Bangkok, Thailand Affiliation of 1 st author * ffisksb@ku.ac.th, bffisksb@yahoo.com Abstract: To increase value added of Cladophora glomerata (SARAI-KAI), an edible green fresh-water alga with high protein and fiber resource from Northern part of Thailand by making healthy food of algal yogurt (AY). This work was focused on yogurt processing line with the high fiber and protein from the algae in the product through the study on customer behavior, improvement of yogurt processing line, its preservation, character and nutrition. From random interviewing of 100 customer (50 male and 50 female), the AY character of customer needed were the fine powder algae with natural color and flavor in AY. From sixteen processing lines through sensory evaluation by the 9-point hedonic scale method, the most acceptance of the composition of AY processing line (45 C, 7 hour incubation) was shown by mixing of cow milk 500 g, yogurt inoculum 36 g, whipping cream 83 g, sweetener 9 g and dried fine pieces of algae (commercial sterilization by stream for 15 min before using) 2 g. Its sensory character score of color, flavor, body/texture, taste and overall acceptability was 7.5, 7.5, 7.3, 6.8, and 7.5, respectively. AY was preserved at least 14 days at 4 C with the acceptance of consumer via sensory evaluation, the amount of microbiological requirement were under the regulation level of Thai standard during testing time. AY characters [mean (unit)±sd, n=3] as ph, aw, susceptibility to syneresis (%), water holding capacity (%), firmness (N), viscosity (cp), cohesiveness, the color as L*, a* and b* were 4.07±0; 0.98±0; 41±0.15; 62.78±0.34; 78.67±0.73; -147±4.01; 1,820±66.64; ± 0.16, ± 0.16 and ± 0.19, respectively. Percentage of carbohydrate, protein, fat, ash, moisture and fiber (by AOAC method; n=3) were ± 0.08, 2.78 ± 0.10, 8.56 ± 0.33, 0.90 ± 0.01, ± 0.89 and 0.21 ± 0.08, respectively. Fiber and protein were higher than the control (AY without algae). Food energy was 3.48 cal/g AY. This AY could represent an alternative way for high fiber and protein. Its named was Yogurtkai-FPRO-KU Science and Technology in the Disruptive Era

117 A_010_PF: EXTRACTION OF SOLUBLE PROTEIN FROM DEFATTED RICE BRAN PLUS RICE MALT Yupakanit Puangwerakul 1 *, Suvimol Soithongsuk 2 1,2 Food Technology Faculty, Rangsit University, PathumThani, Thailand * lombiotec@yahoo.com Abstract: This research investigated the production of value-added protein from defatted rice bran, a by-product from rice bran oil industry by using rice malt as a co-raw material. The objective of this research was to enhance soluble protein extraction and reduce phytic acid by using an enzyme in rice malt in comparison with a conventional alkaline extraction method. The results showed that, without rice malt, the alkaline extraction produced the highest content of soluble protein but could not reduce the amount of phytic acid in the solution, whereas water extraction with the addition of rice malt promoted the decrease of phytic acid at the levels of 40%. Therefore, the ratio of defatted rice bran to rice malt at 1:2 was suitable ratio for protein extraction since it yielded highest soluble protein and lowest phytic acid content. Keywords: Soluble protein, Rice malt, Defatted rice bran, Phytic acid 111 The 44 th Congress on Science and Technology of Thailand (STT 44)

118 A_011_PA: SUPPRESSION OF APOPTOSIS BY YANANG (Tilliacora triandra) IN METHOMYL STIMULATED RAW MACROPHAGE Boonyarit Kukaew 1,2, Wanna Sirisangtragul 1, Sompong Klaynhongsruang 2,3, Nisachon Jangpromma 1,2, * 1 Department of Integrated Science, Forensic Science Program, Faculty of Science, Khon Kaen University 2 Protein and Proteomics Research center for commercial and industrial purposes (ProCCI) Faculty of Science, Khon Kaen University 3 Departments of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand * nisaja@kku.ac.th Abstract: Methomyl or S-methyl N-(methylcarmoyloxy) thioacetimidate is toxic chemical compound that widely used in agriculture. It belongs to the carbamate group, which has been well known in generating reactive oxygen species (ROS) and eventually leading to multi-organ toxicity. In addition, Methomyl has been reported as immunotoxic compound. Therefore, this research aimed to evaluate the detoxification efficiency of Yanang water extract (YWE) toward macrophage (RAW 264.7) cells stimulated by methomyl. At first, the cytotoxicity on Peripheral Blood Mononuclear Cells (PBMCs) and RAW cells of YWE was evaluate by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The results obtained were found μg/ml YWE had no effect on the viability of both PBMCs and RAW cells. On the contrary, the cytotoxicity effect induced by 11,000 μm methomyl to RAW cells was observed as evidence by significantly reduction of cell growth. A pronounced protective effect against this damage was observed upon co-incubation of the cells with 2.5, 5, and 10 µg/ml of YWE. The collected data from dual acridine orange/ethidium bromide fluorescent staining clearly demonstrate that methomyl induces apoptosis in RAW cells, which was evidently decreased by co-treatment with YWE. In conclusion, our research suggested that Yanang extract might contain natural bioactive compounds which play an important role in detoxification of methomyl. Acknowledgements: We would like to thank the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University for their financial assistance. 112 Science and Technology in the Disruptive Era

119 A_012_PA: DETERMINATION OF THE CYTOTOXICITY AND ANTIOXIDANT ACTIVITY IN WILD EDIBLE MUSHROOM Prakaymars Panumars 1,2, Sompong Klaynongsruang 2,3, Nisachon Jangpromma 1,2,* 1 Departments of Integrated Science, Forensic Science Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 3 Departments of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand * nisaja@kku.ac.th Abstract: Mushrooms are widely consumed in many countries. It has reported beneficial effects for human health because they contain full of proteins, rich in vitamins and minerals. However, some species are dangerous because they cause toxicity. Therefore, wild mushroom from the local market in Khon Kaen that are popular in northeastern region of Thailand was subjected to 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay for finding a scientific answer to concerning about their relating to a toxin effect on macrophage (RAW 264.7) cell, human peripheral blood mononuclear cell (PBMC) and human red blood cell (hrbc). The nucleotide sequences identification using DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) was indicated that this mushroom identical to Amanita hemibapha (Hed Ra York Laung). The antioxidant activity of A. hemibapha was evaluated by DPPH scavenging assay compared to ascrorbic acid (Vit C). The results show that crude A. hemibapha water extract (cawe) at the concentration of 12.5 g/ml had antioxidant activity about 70% which was a more than Vit C (20 %). Moreover, the MTT assay results show that cawe at concentration of μg/ml had no cytotoxic effect on PBMCs and hrbc. However, the viability of RAW cells was reduced to 65% after co-cultured with 50 μg/ml cawe. At the concentration of 100 μg/ml, cawe significantly reduced RAW cells viability by 20% after 24 h treatment. The collected data clearly demonstrate that although cawe possess a great antioxidant activity, but the possible risks to RAW cells could be observed at the high concentration. Therefore, experimental and also clinical evaluations are required to identify the possible side effects of wild mushrooms. Acknowledgements: This study was supported by the Research Capability Enhancement Program through Graduate Student Scholarship, Faculty of Science, Khon Kaen University. The study was further financial supported by Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindrorn : Khon Kaen University and also the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, Thailand. 113 The 44 th Congress on Science and Technology of Thailand (STT 44)

120 A_013_PA: CARYOPHYLLENE OXIDE EXTRACT FROM Ocimum STRAW TRUNK Thanaporn Wichai, Sarintip Sooksai, Ruengwit Sawangkeaw, Sajee Noitang, Weradaj Sukaead, Aphichart Karnchanatat* The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, THAILAND * Abstract: Ocimum is an aromatic herb with edible leaves and seed. Every year, kg/rai of Ocimum straw trunk ( stems and inflorescence) was produced as an agricultural waste during seed production process. Therefore, adding value to this waste become interested. In this research, essential oil was extracted from Ocimum straw trunk by Supercritical CO 2 technique. This technique is environmental friendly and widely used for extracting phyto-bioactive compounds. The chemical compositions of essential oil from Ocimum straw trunk was characterized by Gas Chromatography-Mass Spectrometry ( GC-MS), caryophyllene oxide was discovered as the major composition. The maximum amount of caryophyllene oxide found in this essential oil extract was 7.25% of area sum at retention time (RT) min, and Retention Index (RI) was when the chromatogram and mass spectrum were compared to the NIST 2011 data ( Figure 1.) Caryophyllene oxide is a bioactive compound that can inhibit bacterial and fungal growth, and proliferation of cancer cell. Figure 1. a. Chromatogram to extract from hairy basil straw using Supercritical CO 2 technique. b. Mass spectrum for caryophyllene oxide at RT min and RI as comparing to Kovats Retention Index as c. NIST Standard Reference Database Keyword: Ocimum Straw Trunk, Supercritical CO 2, caryophyllene oxide 114 Science and Technology in the Disruptive Era

121 A_014_PA: EFFECT OF Ocimum SEED POWDER TOWARD OIL ADSORPTION AND CHANGE OF FOOD COLORING Sajee Noitang, Weradej Sukaead, Ruengwit Sawangkeaw, Sarintip Sooksai* The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok, THAILAND * Abstract: Ocimum seed powder is a processed product made with supercritical carbon dioxide extraction. The components of Ocimum seed powder are carbohydrate ( %), fiber ( %), protein ( %), ash ( %), moisture ( %) and fat ( %). Study of Ocimum seed powder s oil adsorption, in comparison to other products, including chitosan, gum arabic, xanthan gum and guar gum reveals that chitosan and gum arabic yield the highest oil adsorption rate (2.94 g oil/g) while non-sterile Ocimum seed powder yields higher oil adsorption rate (2.76 g oil/g) than the steriled Ocimum seed powder (2.65 g oil/g), xanthan gum (2.54 g oil/g) and guar gum (2.38 g oil/g). Another experiment tests adsorption of food coloring with Ocimum seed powder, testing green, yellow and red food coloring (food coloring solution with the concentration of 1 μl/ml) for an incubation period of 7 days. This experiment reveals that the tones of green and yellow food coloring solution are slightly darker and then remain stable. Meanwhile, the tone of red food coloring solution is somewhat lighter (Figure 1). Therefore, the result suggests that the Ocimum seed powder has the oil adsorption rate of 2.65 g oil/g and does not affect the color of food products that use green and yellow food coloring; however, Ocimum seed powder will lighten the color of food products that use red food coloring. Figure 1. Adsorption of food coloring with Ocimum seed powder Keyword: Ocimum seed powder, oil adsorption, color adsorption 115 The 44 th Congress on Science and Technology of Thailand (STT 44)

122 A_015_OF: SMART SYSTEM FOR NFT HYDROPONICS FARM Sirichai Khoukitpaisal, Pasin Rattanasin, Pattariya Singpant, Teeravisit Laohapensaeng, Suppakarn Chansareewittaya* School of Information Technology, Mae Fah Luang University, Thailand * Abstract: The purpose of this paper is to develop smart system for adjust nutrient automatically in Nutrient Film Technique (NFT) hydroponics farm. According from the daily life of farmers, they have to monitor and control Potential of Hydrogen ion (ph) and Electrical Conductivity (EC) value appropriate for plants by themselves. They spend a lot of time to take care plants. Thus, the system which aims to help farmer adjust nutrient is developed. The analogue ph meter and analogue EC meter are used to measure the environment of the hydroponic farm. The microcontroller board which is named Arduino is used to get data from sensors and send data to IoT board which is named Raspberry pi. After that, raspberry pi sends data packet to a server. The control module which is included nutrient pump can be controlled by touch screen monitor. Moreover, the information can be monitored on website and line bot. 116 Science and Technology in the Disruptive Era

123 A_016_OA: ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF ENDOPHYTIC FUNGI ISOLATED FROM Polyscias fruticosa AGAINST SOUTHERN BLIGHT Chutima Tanapichatsakul, 1 Patcharee Pripdeevech 1,2,* 1 School of Science, Mae Fah Luang University, Chiang Rai, Thailand 2 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Chiang Rai, Thailand * patcharee.pri@mfu.ac.th Abstract: Many recent studies have been demonstrated that endophytic fungi from host plant were applied significantly for inhibiting of plant diseases. Polyscias fruticosa has been known as source of bioactive compounds with medical properties and used as a natural antibiotic and immunity booster in Thailand. A total of 35 endophytic fungi (PF1-PF35) were isolated from P. fruticosa leaves. All isolates were cultured in potato dextrose agar for purifying and classifying by using their morphology. The fungal isolates were categorized into five genera including Aspergillus, Colletotrichum, Nigrospora, Ustilago and Diatrypella. All strains were cultured in potato dextrose broth for 30 days prior extraction with ethyl acetate organic solvent. All extracts were concentrated and tested its antibacterial activity against Sclerotium rolfsii (southern blight disease in tomato) by using agar disc diffusion assay. Moderate antifungal activity was observed among these crude extracts with % inhibition ranging from 42.04% to 72.66%. In addition, the greatest antifungal activity was obtained from crude extract of endophytic fungus Diatrypella PF35 with %inhibition of 70.66%. The newly isolated Diatrypella PF35 could be exploited as a potential source of bioactive compounds and plant defense activators. 117 The 44 th Congress on Science and Technology of Thailand (STT 44)

124 A_017_OA: ANTIFUNGAL ACTIVITY OF CRUDE EXTRACTS OF ENDOPHYTIC FUNGI ISOLATED FROM Litsea Petiolata AGAINST Pestalotiopsis samarangensis Sarunpron Khreungsai, 1 Patcharee Pripdeevech 1,2,* 1 School of Science, Mae Fah Luang University, Chiang Rai, Thailand 2 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Chiang Rai, Thailand * patcharee.pri@mfu.ac.th Abstract: The endophytic fungi has been used as biocontrol against plant diseases. This study was aimed to evaluate antifungal activity of crude extracts of endophytic fungi isolated from L. petiolata leaves. Sixty-six endophytes were isolated from L. petiolata leaves. They were cultured for four weeks prior the chemical composition was extracted by ethyl acetate. All crude extracts were screened their antifungal activity by using disc diffusion assay against Pestalotiopsis samarangensis that was detected as plant pathogen causing rose apple deterioration. Among all endophytic extracts, strong antifungal activity were detected in three crude extracts including strain MFLUCC , strain MFLUCC and strain MFLUCC with inhibition percentage ranging %. The antifungal activity in term of % inhibition of three selected endophytes was also investigated by dual culture method. As the results, the significant antifungal activity was detected in the crude extract of endophytic fungus MFLUCC inhibiting P. samarangensis with inhibition percentage of These findings showed that the endophytic fungus MFLUCC has the potential to produce active compounds using natural fungicide against plant pathogenic fungi. 118 Science and Technology in the Disruptive Era

125 A_018_PA: THE FEASIBILITY OF USING SPENT COCONUT WATER AS SUPPLEMENT FOR BACTERIAL CELLULOSE PRODUCTION Vatcharin Suwannit, 1,2 Worawut Sevegoon, 1,2 Sunita Chamyuang, 1,2 Natsarun Saichana, 1,2 Natthawut Yodsuwan 1,2 * 1 School of Science, Mae Fah Luang University, Thasud, Chiang Rai 57100, Thailand 2 Microbial Products and Innovation Unit, Mae Fah Luang University, Thasud, Chiang Rai 57100, Thailand * natthawut.yod@mfu.ac.th Abstract: Bacterial cellulose (BC) is widely used in various applications. To apply in biomedical use, the natural BC and low-cost production should be considered. The aim of this study was to apply the spent coconut water (SCW) as supplement in the culture medium to produce high yield of BC. An acetic acid bacterium, Komagataeibacter nataicola (formerly Acetobacter sp. TISTR 975), was producer. The culture medium was modified from Watanabe and Yamanaka (1995) in which the molasses was used as carbon source. The culture was performed statically in 600-mL plastic boxes containing 200 ml culture medium at 30 C for 14 days. The effect of 50% (v/v) SCW and 50% (v/v) fresh coconut water (FCW) supplemented in the culture medium containing an initial total carbohydrate at 43.4±15.9 g/l as carbon source were studied. Besides, the control conditions including 100% (v/v) SCW and 50 g/l sucrose as a sole carbon source, respectively, were also investigated. The results showed that the addition of 50% (v/v) FCW showed the maximum BC concentration (C P), BC productivity (Q P) and % Yield at 13.5±0.1 g/l, 1.8±0.0 g/l d and 23.7±0.1%, respectively. Interestingly, the results were not different significantly (p 0.05) with the addition of 50% (v/v) SCW. The maximum C P, Q P and % Yield were 12.5±0.1 g/l, 1.6±0.0 g/l d and 31.9±4.7%, respectively. Undoubtedly, both supplements provided the greater yield than the controls. Thus, the SCW could be an alternative supplement in the culture medium containing molasses as carbon source to promote the BC production by K. nataicola. 119 The 44 th Congress on Science and Technology of Thailand (STT 44)

126 A_019_OA: GENOME-WIDE ANALYSIS OF THE DOF GENE FAMILY IN DURIAN REVEALS RIPENING-ASSOCIATED AND CULTIVAR-DEPENDENT DOF TRANSCRIPTION FACTORS Gholamreza Khaksar 1, Supaart Sirikantaramas* 1,2 1 Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand 2 Omics Sciences and Bioinformatics Center, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand * supaart.s@chula.ac.th Abstract: DNA binding with one finger (Dof) proteins constitute a ubiquitous plant-specific transcription factor (TF) family associated with numerous biological processes, such as ripening. However, our actual knowledge regarding Dof TFs in fruit is almost scarce. We conducted a genome-wide analysis of durian (Durio zibethinus M.) cv. Musang King and identified 24 durian Dof (DzDof). Alignment of DzDof sequences revealed a highly conserved domain of 50 amino acids, uniformly observed at the N-terminus. All DzDofs harbor nuclear localization signal as predicted by in silico analysis. Out of the 24 DzDofs, 13 were fruit specific. Notably, fruit-specific DzDofs exhibited different expression patterns during ripening. Four ripening-associated Dofs (DzDof2.1, DzDof5.4, DzDof 5.6, and cyclic DzDof2) were identified, which expressed increasingly during postharvest ripening in Monthong and Chanee cultivars. However, DzDof5.4 was expressed at significantly higher levels in Chanee. Gene coexpression analysis of DzDof5.4 ortholog (Arabidopsis Dof5.4) revealed its role in mediating auxin-regulated transcription. Among nine non-ripening-associated Dofs, DzDof2.2 is cultivar dependent and was expressed at higher levels in Chanee. Higher expression of DzDof2.2 could enhance auxin levels. The higher expression levels of DzDof2.2 and 5.4 in Chanee could contribute to faster ripening through earlier ethylene response (auxin-ethylene crosstalk), when compared to Monthong. 120 Science and Technology in the Disruptive Era

127 A_020_PF: CORRELATION BETWEEN DNJ CONTENT AND MLX56 GENE EXPRESSION IN MULBERRY LEAF Onpawee Sangsai, 1 Sudatip Ruttanawijit, 2 Wannarat Phonphoem, 2,3 Ratree Wongpanya, 2,3 Amornrat Promboon, 2,3* 1 Interdisciplinary Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok, Department of Biochemistry, Faculty of Science, Kasesart University, Bangkok, Center for Excellence in Silk, Kasetsart University, Kamphang Saen Campus, Nakorn Pathom, * fsciarp@ku.ac.th Abstract: DNJ is one of the most potent -glucosidase inhibitors that can help lowering blood sugar level in diabetes type II patients. DNJ content of various mulberry cultivars in Kasetsart University ranged from 0.05 to 0.74 g/100g (dry weight). Mulatexin (MLX56) gene was successfully amplified from mulberry leaf by RT-PCR. The transcriptional level of MLX56 was correlated with the DNJ content in mulberry leaf. Thus, this gene may be used as an indicator for determining the DNJ content in mulberry leaf during the DNJ induction by chemicals and/or hormones. 121 The 44 th Congress on Science and Technology of Thailand (STT 44)

128 A_021_OF: SULFUR AVAILABILITY IN A TYPIC PALEUSULT AMEDED WITH DIFFERENT SULFUR RICH SOIL CONDITIONERS Nuttharuja Hirunburana 1, Suphicha Thanachit 1*, Somchai Annusontpornpern 1, Irb Kheoruenromne 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * agrspc@ku.ac.th Abstract: This study on sulfur (S) availability in a cassava growing soil, Typic Paleusult (Warin soil series) and the response of cassava to different S fertilizer sources was carried out in pot experiment under greenhouse condition. Completely randomized design with three replications was employed. There were four treatments: control without no fertilizer added, sole application of NPK fertilizer, and NPK fertilizer applied with two S fertilizer sources, gypsum and ammonium sulfate (NH 4SO 2). The NPK fertilizer was applied at the rates of 100:50:100 kg N:P 2O 5:K 2O ha -1 and S fertilizer was added at the rate of 125 kg S ha -1. All were applied at one month after cassava, Huay Bong 80 variety, was planted. Cassava was harvested at 4 months of age. Soil sample was collected at 1, 3, 5, 7, 15, 30, 45, 60 and 90 days after S fertilizer application for determining the contents of soluble-, organic- and available S. Results revealed that NH 4SO 2 dissolved in water more easily than did gypsum. Total dissolved S content of 31.7 g kg -1 obtained from NH 4SO 2 was significantly greater than that from gypsum (27.0 g kg -1 ) but when incorporated them into the soil, the higher content of S was found in gypsum-applied soil (299 mg kg -1 ) than in NH 4SO 2-added soil (258 mg kg -1 ). The application of S in the form of neither NH 4SO 2 nor gypsum had significant effect on cassava s aboveground biomass ( g pot -1 respectively) and S uptake ( mg pot -1 respectively). Ammonium sulfate gave higher content of available S in the soil than did gypsum only within 15 days after applying ( compared to mg kg -1 ), after that the amount was not different. Therefore, cassava took up S rapidly at the early growth stage within one month after applying. Water soluble S was found in the smallest proportion in all treatments. The soil with no application of S fertilizer contained the highest organic S. Applied S fertilizer induced most of S to become available, especially in the case of NH 4SO 2 addition. Keywords: sulfur, sulfur availability, sulfur fertilizer, low fertile soil, cassava 122 Science and Technology in the Disruptive Era

129 A_022_OF: EFFECT OF CASSAVA TAILS AND STALK AND POTASSIUM ON CASSAVA, HUAY BONG 80 VARIETY, GROWN IN WARIN SOIL SERIES Thanaphon Jenwitheesuk, 1,* Somchai Anusontpornperm, 1,* Suphicha Thanachit and Irb Kheoruenromne 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, THAILAND * . t.jenwitheesuk@gmail.com (T. Jenwitheesuk), somchai.a@ku.ac.th (S. Anusontpornperm) Abstract: The study was undertaken in a farmer field Ban Supplu Noi, Huay Bong subdistrict, Dan Khun Thot district, Nakhon Ratchasima province to investigate the effect of cassava tails and stalk (CTS) and potassium (K) in Warin soils series (Typic Paleustults) that had low fertility status. Split plot with five main plots, comprising the application of 0, 3.125, 6.25, 12.5 and 25 t ha -1 of CTS, and six subplot being composed of the addition of 0, 25, 50, 75, 100 and 125 kg ha -1 of K 2O was employed with four replications. Cassava was planted at nearly the end of rainy season and harvested at 12 months of age. Result showed that the application of CTS at the rate of 25 t ha -1 highly significantly promoted the highest fresh tuber yield of t ha -1 whereas other treatments with zero application of CTS and those applied at the rates between t ha -1 gave inferior yield in the range of t ha -1. The same trend was also found in the case of starch yield of which the soil amended with CTS at the rate of 25 t ha -1 highly significantly stimulated the highest amount of t ha -1. The application of 100 kg ha -1 of K 2O highly significantly induced the highest fresh tuber yield of t ha -1, however, it was not statistically different from those applied with 75 and 100 kg ha -1 of K 2O that gave this yield of and t ha -1, respectively. The same trend was also found in the case of starch yield of which K fertilization at the rate of 100 kg ha -1 highly significantly stimulated the highest amount of t ha -1. Increasing rate of K also highly significantly increased stem, stem base and aboveground biomass. The interaction between CTS and K revealed that with no application of CTS, K fertilization at the rate of 125 kg ha -1 of K 2O highly significantly stimulated the highest fresh tuber yield of t ha -1 followed by and t ha -1 which were obtained from the soil treated with 25 t ha -1 of CTS together with 100 kg ha -1 of K 2O, and 25 t ha -1 of CTS together with 25 kg ha -1 of K 2O, respectively. Keywords: cassava tails and stalk, soil amendment, cassava, low fertile soil, potassium 123 The 44 th Congress on Science and Technology of Thailand (STT 44)

130 A_023_OF: EFFECT OF CALCIUM FROM DIFFERENT SOURCES ON CASSAVA GROWN IN WARIN SOIL SERIES Naphatsanan Somngam 1, Suphicha Thanachit 1,*, Somchai Anusontpornperm 1 and Irb Kheoruenromne 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * agrspc@ku.ac.th Abstract: The study on the effect of calcium (Ca) from different sources on cassava was carried out in low Ca content with strongly acidic Warin soil series in Nakhon Ratchasima province. The 2 4 factorial in randomized complete block with four replications was employed. Treatments comprised two sources of Ca fertilizer, ground limestone (CaCO 3) and calcium chloride (CaCl 2), and four rates of 0, 50, 100 and 200 kg Ca ha -1. In a separate experiment conducted in laboratory, the content of dissolved Ca from Ca fertilizers and soil amended with and without different Ca fertilizer sources within 480 h was compared. All Ca fertilizers applied at same rate of 200 kg Ca ha -1. Results revealed that CaCl 2 was completely dissolved in water within 120 min while CaCO 3 was almost dissolved in water at the stirring time of 2,250 min. Total Ca content of 695 mg kg -1 dissolved from CaCO 3 was significantly greater than that from CaCl 2 (170 mg kg -1.). When applied both materials to the soil, significantly lowest soil available Ca content was found but with no statistical difference to that of the control. The application of Ca fertilizer at different rates and from different sources had no effect on yield components of cassava. Using CaCO 3 as a source of Ca resulted in greater aboveground biomass (15.2 t ha -1 ) and yields (35.4 and 9.91 t ha -1 for fresh tuber and starch yields, respectively) than did the use of CaCl 2. Considering the rate of Ca, the addition of 100 kg Ca ha -1 gave the highest contents of aboveground biomass (16.3 t ha -1 ), fresh tuber yield (36.5 t ha -1 ) and starch yield (10.1 t ha -1 ). Keywords: Cassava Huay Bong 80 variety, Loamy sand soil, Ca rich soil conditioner 124 Science and Technology in the Disruptive Era

131 A_024_OF: RESPONSE OF CASSAVA TO DIFFERENT RATE AND TYPE OF MAGNESIUM SOURCES IN SATUK SOILS SERIES Apitsara Charoenphon 1, Suphicha Thanachit 1,*, Somchai Anusontpornperm 1 and Irb Kheoruenromme 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * agrspc@ku.ac.th Abstract: The study on the response of cassava, Huay Bong 80 variety, to different rate and type of magnesium (Mg) fertilizer sources was carried out in a low available Mg status, Satuk soil series in Nakhon Ratchasima province. The 2 4 factorial in randomized complete block with four replications was employed. Treatments comprised two sources of Mg fertilizer including dolomite and MgCl 2 with four rates of 0, 50, 100 and 200 kg Mg ha -1 applied. In a separate experiment, the content of dissolved Mg from Mg fertilizers and soil amended with and without different Mg fertilizer sources was investigated in laboratory for 48 h under control condition. All Mg fertilizers were applied at the same rate of 200 kg Mg ha -1. Results revealed that MgCl 2 and dolomite almost dissolved all in water at the stirring time of 720 and 1,440 min, respectively, indicating that MgCl 2 was more soluble than dolomite. Dolomite significantly gave the greater dissolved Mg content than did MgCl 2 with the value of 43.8 compared to mg kg -1. However, both Mg fertilizer sources statistically increased available Mg content in soils where the lowest content of mg kg -1 was found in the soil without Mg fertilizer applied. Cassava clearly responded to additional Mg fertilizer in terms of above ground biomass, fresh tuber yield and starch yield. Although there was no difference the response of cassava to MgCl 2 and dolomite as a source of Mg, the application of Mg fertilizer clearly gave higher yield components than did no application of this fertilizer with the rates of kg Mg ha -1 giving no statistical difference in this context whereas the highest contents of aboveground biomass (15.89 t ha -1 ), fresh tuber yield (37.98 t ha -1 ) starch content (28.76%) and starch yield (6.41 t ha -1 ) were obtained from the soil added with 200 kg Mg ha -1. Keywords: dolomite, magnesium chloride, loamy sand soil, cassava 125 The 44 th Congress on Science and Technology of Thailand (STT 44)

132 A_025_OF: EFFECT OF RICE HUSK ASH AND NITROGEN ON GROWTH AND YIELD OF CASSAVA, HUAY BOMG 80 VARIETY, IN YASOTHON SOIL SERIES. Pinate Ruenchan 1*, Somchai Anusontpornperm 1*, Suphicha Thanachit and Irb Kheoruenromne 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand * teemsn@hotmail.com (P.Ruenchan), somchai.a@ku.ac.th (S. Anusontpornperm) Abstract: Field experiment was undertaken in a farmer field at Ban Supplu Noi, Huay Bong subdistrict, Dan Khun Thot district, Nakhon Ratchasima province objectively to investigate the effect of rice husk ash (RHA) and nitrogen (N) on yield of cassava, Huay Bong 80 variety. Representative soil in the area was Yasothon Soil Series (classified as Typic Paleustult). Experimental design was arranged in split plot with five main plots and six subplots, having four replications,. Main plots comprised the use of rice husk ash as soil amendment at the rates of 0, 3.125, 6.25, 12.5 and 25 t ha -1 while subplot consisting of N fertilization at the rates of 0, 25, 50, 75, 100 and 125 kg ha -1. Cassava was planted at nearly the end of rainy season and harvested at 12 month of age. Result showed that the soil treated with RHA at the rate of 25 t ha -1 (RHA 125) highly significantly gave the highest cassava fresh tuber yield and starch yield of and t ha -1 but with no statistical with that applied with 12.5 t ha -1 of RHA (RHA 12.5). These two rates of RHA also significantly induced the highest aboveground plant parts. The addition of N at the rate of 100 and 125 kg ha -1 (N 100 and N 125) highly significantly promoted the highest fresh tuber yield of and t ha -1, respectively, and the application at both rates also highly significantly stimulated the highest starch yield of t ha -1. Increasing rate of N application significantly elevated the amount of aboveground plant parts. Neither RHA nor N had clear impact of cassava starch content. The interaction between RHA and N statistically showed no different impact on cassava yield and plant components, however, the combination between RHA applied at the rate of 25 t ha -1 and N added at the rate of 100 kg ha -1 (RHA 25N 100) tended to induce the highest starch yield of t ha Science and Technology in the Disruptive Era

133 A_026_OF: RESPONSE OF CASSAVA GROWN IN A LOAMY SAND SOIL TO BENTONITE AND CHEMICAL FERTILIZER Saovanee Boonrod 1, Suphicha Thanachit 1,*, Somchai Anusontpornperm 1, Irb Kheoruenromme 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * agrspc@ku.ac.th Abstract: A study on the effect of bentonite and chemical fertilizer on cassava was conducted in Warin soil series, loamy sand topsoil variant, in Nakhon Ratchasima province. Split plot design with four main plots and four subplots was employed, having three replications. Main plot consisted of 0, 1.25, 2.5 and 5.0 t ha -1 of bentonite applied as soil amendment during land preparation. Subplot was composed of 0:0:0, 50:25:50, 100:50:100 and 200:100:200 kg N:P 2O 5:K 2O ha -1. Cassava was harvested at 10 months old. In the other experiment, N, P and K contents released in the soil incubated with bentonite at different rates for 270 days were examined. Results showed that there was no different soil K ( mg kg -1 ) and N contents ( mg kg -1 ) released until day-270 where the soil amended with bentonite at the rate of 1.25 t ha -1 significantly released the highest available N content of 24.7 mg kg -1. Bentonite clearly increased available P released from the soil which the significantly greatest content in the range of mg kg -1 was detected in the soil amended with 5 t ha -1 of this soil amendment. The addition at the rate of 1.25 t ha -1, however, gave the same result as the control. In addition, bentonite released N and P to the soil for more than 270 days which was almost throughout cassava growing period. Cassava grown on the soil without soil amendment added nor chemical fertilizer applied statistically gave the lowest fresh tuber yield (11.9 t ha -1 ), starch yield (3.06 t ha -1 ) and aboveground biomass (3.66 t ha -1 ). The additions of 100:50:100 and 200:100:200 kg N:P 2O 5:K 2O ha -1 statistically identically gave the highest cassava yield components. The application of 200:100:200 kg N:P 2O 5:K 2O ha -1 along with 1.25 t ha -1 of bentonite as soil amendment induced the highest cassava fresh tuber yield (43.7 t ha -1 ), starch yield (12.4 t ha -1 ) and aboveground biomass (15.8 t ha -1 ). Applying bentonite and chemical fertilizer showed no different impact on starch content in cassava tuber ( %) Keywords: sandy soil, soil amendment, chemical fertilizer, nutrient release 127 The 44 th Congress on Science and Technology of Thailand (STT 44)

134 A_027_OF: EFFECT OF RICE HUSK ASH ON VIRING CANE GROWN IN LOAMY SAND SATUK SOIL SERIES Souksady Insixiengmay 1, Suphicha Thanachit 1,*, Somchai Anusontpornperm 1, Irb Kheoruenromme 1 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * agrspc@ku.ac.th Abstract: Coarse textured soils, having low soil water holding capacity and soil fertility status, are widely distributed in northeastern region of Thailand and widely used for sugarcane crop cultivation. This study was conducted to determine the response of two sugarcane varieties, K95-84 and Khon Kaen 3 (KK3) to rice husk ash (RHA) used as soil amendment in a loamy sand soil classified as Satuk soil series in Nakhon Ratchasima province. Randomized complete block design with four replications was employed. Treatment consisted of four rates of RHA, 0, 6.25, 12.5 and 25 t ha -1. This waste was incorporated into the soil during land preparation. Result revealed that virgin cane of K95-84 variety showed no response to different rates of RHA in terms of cane yield ( t ha -1, No. ha -1 ), commercial cane sugar, CCS ( ), sugar yield ( t ha -1 ), cane diameter ( mm) and length ( m), and number of node (28-29 No. cane -1 ). Rice husk ash clearly enhanced the growth and virgin cane yield of sugarcane KK 3 variety with the application of RHA at the rate of t ha -1 significantly inducing the highest sugar yield and number of cane in the ranges of t ha -1 and 55,556-60,762 No. ha -1, respectively, and these ranges were statistically higher than those obtained from the control with no RHA application. However, with no statistical difference, the highest virgin cane yield (100 and 110 t ha -1 ) and CCS (17.37 and 19.00) of K95-84 and KK3 varieties was obtained from the soil amended with RHA at the rate of 25 t ha Science and Technology in the Disruptive Era

135 A_028_OF: RESPONSE OF CASSAVA, KU 50 AND HUAY BONG 80 VARIETIES, GROWN IN SATUK SOIL SERIES TO CALCIUM AND NITROGEN Teeradon Kardudom, 1,* Somchai Anusontpornperm, 1,* Suphicha Thanachit, 1 Irb Kheoruenromne 1 Department of Soil Science, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand * teeradon4114@outlook.com (T. Kardudom), agrsca@ku.ac.th (S. Anusontpornperm) Abstract: The study was carried out in a farmer field at Ban Supplu Noi, Huay Bong subdistrict, Dan Khun Thot district, Nakhon Ratchasima province objectively to compare yield response between cassava two varieties, KU 50 and Huay Bong 80, grown in Satuk soil series to calcium (Ca) and nitrogen (N). Experimental design was arranged in 2x4x4 factorial in randomized complete block plot with four replications. The first factor comprised two varieties of cassava, KU50 and Huay Bong 80. The second factor consisted of four rates of calcium; 0, 50, 100 and 200 kg ha -1 of Ca while the third factor including four rates of nitrogen; 0, 50, 100 and 200 kg ha -1 of N. Calcium chloride was used as a source of Ca while urea and diammonium phosphate being as a source of N in this study. Phosphorus and potassium were also applied at the rate of 60 and 120 kg ha -1 of P 2O 5 and K 2O to all plots. Fertilization was made at 2 months after planting and cassava was planted at the end of rainy season. The plant was harvested at 12-month of age and plant components were also measured at the harvesting time. Results showed that KU 50 and Huay Bong 80 varieties produced nearly identical amount of fresh tuber and starch yields while the former highly significantly giving greater amounts of all aboveground plant parts and the latter having higher starch content (29.29%). Cassava fresh tuber and starch yields highly significantly responded to the application of 100 kg ha -1 of Ca, having the greatest amounts of and 9.98 t ha -1, respectively, with no statistical difference to that of the rate of 200 kg ha -1 of Ca. However, the use of Ca had no clear influence on aboveground plant parts of cassava. The addition of 200 kg ha -1 of N promoted the highest fresh tuber yield and starch yield of and t ha -1, respectively, however, these were not statistically different from that applying 100 kg ha -1 of N (32.56, 9.41 and t ha -1, respectively). It was clear that the control with no application of Ca and N the plant significantly produced the lowest fresh tuber and starch yields, and also the poorest aboveground growth. Additionally, with no statistical difference, growing KU 50 variety in this Satuk soil series with the addition of 100 kg ha -1 of Ca together with N at the rate of 100 kg ha -1 of N, the highest fresh tuber and starch yields of and t ha -1 were obtained. 129 The 44 th Congress on Science and Technology of Thailand (STT 44)

136 A_029_OF: NITROGEN UTILIZATION EFFICIENCY AND METABOLISM OF DAIRY GOATS FED POMEGRANATE SEED PULP AND SOY BEAN OIL Jongkolnee Yaowapaksophon* Department of Animal Production Technology and Fisheries, Faculty of Agricultural Technology, King Mongkut s Institute of Technology Ladkrabang, Bangkok 10520, Thailand * jongkolnee.ya@kmitl.ac.th Abstract: The objective of this research was to investigate the effects of supplementing two feedstuffs, pomegranate seed pulp (PSP) or soybean oil (SO) on N balance and utilization efficiency of Saanen dairy goats. Eight lactating cross-bred dairy goats were assigned to receive diets in a replicated 4 4 Latin square design with 4 periods of 14 d adaptation and 7 d of data and sample collection. The four dietary treatments were unsupplementation (control), supplementation with pomegranate seed pulp (PSP) at 120 g/kg DM, supplementation with soybean oil (SO) at 50 g/kg DM, and supplementation with SO at 50 g/kg DM and PSP at 120 g/kg DM (PSPSO), of total dry matter intake. Protein intake, N intake, feces N excreta, milk N, retain N, of goats were not affected (p > 0.05) by dietary treatments. Milk urea nitrogen (MUN) and blood urea nitrogen (BUN) of goat tended to (p = 0.055, p = 0.060) decrease by the use of PSPSO diet. Milk N as proportion of nitrogen intake tended to (p = 0.055) decrease by the use of PSPSO diet, which reflect better N utilization and retain N enhancement (6.9 g/d) or then amino acid absorption improvement in the small intestine and improved N efficiency. Depression of urinary N loss shifted fecal N excreta and then improvement of microbial protein synthesis and enhanced N efficiency. In conclusion use of combination between pomegranate seed pulp and soybean oil of goat diet enhanced N utilization efficiency for milk N and protein. 130 Science and Technology in the Disruptive Era

137 A_030_PF: POTENTIAL OF NATIVE Azotobacter spp. FROM RHIZOSPHERE ON ANTIFUNGAL ACTIVITY AGAINST Fusarium oxysporum IN LABORATORY Marisa De Bels 1,* 1 Department of Applied Science, Faculty of Science and Technology, Loei Rajabhat University, Loei, 42000, Thailand * marphirom@yahoo.com, marphirom@gmail.com Abstract: This extending research aimed to investigate the potential of 90 indigenous strains of Azotobacter spp. isolated from 29 plant soils against Fusarium oxysporum in vitro condition by Completely Randomized Design. All bacterial strains were evaluated for percentage inhibitions of radial growth as their antagonistic activities. The significant difference in antifungal activities against F. oxysporum was found ranging from to 95.72%. In this study, 16 isolates (17.78%) had the highest antagonistic activities of 95.72% similar to those of 65 isolates (72.22%), but higher than those of 9 strains (10%) which gave antagonism action ranging of to %. Among them, the higher antifungal activities had high density and colored colonies with abundant slime. The studies revealed that the antagonism of native azotobacter against F. oxysporum varied associated with strains, bacterial genetics and antagonistic biotic interactions. 131 The 44 th Congress on Science and Technology of Thailand (STT 44)

138 A_031_OA: EFFECT OF CONVERSION OF PADDY FIELD INTO OIL PALM CULTIVATION ON SOIL ORGANIC CARBON Prachwanee Pibumrung* and Pechpikul Khumon 1,2 Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Thailand, * Abstract: Oil palm has been considered one of the most valuable cash crop in Thailand. It is important to understand how cultivation of this perennial crop will affect the soil organic carbon (SOC) in the long term. The aim of this study was to evaluate the changes in soil C stocks after the conversion of paddy fields into oil palm cultivation in Pathum Thani province. Four different land uses types were paddy field, oil palm, banana and oil palm with banana intercropping of small holder farmers. Soil Samples were collected from three plots of 50x50 m 2 of each different land uses at 0-30 cm of soil depth. The collected soil samples were analyzed for soil organic carbon (SOC). Mean SOC ranged from ± 8.84 tc ha -1 to ± tc ha -1 in the 5 th year and ± 9.05 tc ha -1 to ± 8.31 tc ha -1 in the 6 th year with significant differences among values of the different land use type (p 0.05). SOC in oil palm land use increased by % when compared with the paddy field stock in the 5 th year and the 6 th year after conversion. 132 Science and Technology in the Disruptive Era

139 SESSION B: BIOLOGICAL SCIENCE (B1: BIOCHEMISTRY) 133 The 44 th Congress on Science and Technology of Thailand (STT 44)

140 B1_001_OA: KT2 AND RT2 ANTIMICROBIAL PEPTIDES MODIFIED FROM SIAMESE CROCODILE LEUCROCIN I, PROMOTES ANTICANCER ACTIVITY IN HUMAN COLON CANCER HCT-116 CELLS Surachai Maijaroen 1,2, Nisachon Jangpromma 2,3, Jureerut Daduang 4, and Sompong Klaynongsruang 1,2, * 1 Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 3 Department of Integrated Science, Forensic Science Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 4 Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand * somkly@kku.ac.th Abstract: The antimicrobial peptides are regarded as a potential solution for treating cancer cells. Therefore, the current study aimed to investigate the effect of potent cationic antimicrobial peptides, Leucrocin I modified KT2 and RT2, against the HCT-116 colon cancer cells. Using MTT assay, results demonstrated that of peptides KT2 and RT2 exhibited strong cytotoxic effect against HCT-116 human colon cancer cells with IC 50 values of and μg/ml after 24 h treatments, respectively. However, showing no signs of cytotoxicity towards noncancerous Vero cells. Additionally, the colony count indicated that peptides (KT2 and RT2) induced a dose-dependent decrease in the ability of colony formation of HCT-116 cells. The mechanism involved in cancer cell cytotoxicity of both peptides was shown to be associated with the induction of apoptosis, as evidenced by AO/EB fluorescent staining and annexin V-FITC/PI staining flow cytometry analysis. These evidence were due to peptides consequently occurred via suppression of Bcl-2 and XIAP, and triggering p53, cytochrome c, and caspase cascade mrna expression level. Furthermore, the peptides induced cell cycle arrest via up-regulation of cyclin-dependent kinase inhibitor p21. These collected data clearly demonstrated that peptides KT2 and RT2 offered great potential in the treatment of human colon cancer cells. 134 Science and Technology in the Disruptive Era

141 B1_002_OF: EFFECT OF Morinda citrifolia L. ROOT ETHANOLIC EXTRACT ON SK-N-SH NEUROBLASTOMA CELLS INDUCED BY H 2O 2 Srisawad 1, Apinun limmongkon 1, Pornpat samang 2 and Damratsamon Surangkul 1, * 1 Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phisanulok 65000, Thailand Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phisanulok 65000, Thailand * damratsamon@gmail.com Abstract: Alzheimer s disease (AD) is the most common form of dementia in the people having age more than 65. The risk factor of Alzheimer s including genetics alteration, aging and environments may also be important such as pollution, smoking and UV radiation that have been involved in oxidative stress leading to neuronal cell death by generation of reactive oxygen species (ROS) such as hydrogen peroxide (H 2O 2). Therefore, therapeutic strategies aimed at preventing ROS production for the treatment of AD. In this study focus on Molinda citrifolia L. or noni. Noni has been used in traditional medicine for anti-inflammation, anti-virus, antioxidation, and neuroprotection. Thus, this study aims to investigate neuroprotective effect of noni on SK-N-SH neuroblastoma cells induced by H 2O 2. The crude extracts from root were extracted by ethanol and were assessed for neuroprotective effect. Incubation of SK-N-SH cells pre-treated with various concentrations of noni root ethanolic extract for 24 h and then treated with 175 µm H 2O 2 for 3 h measured cell viability by MTT assay. The result showed that neuroprotective effect of noni root ethanolic extracts significantly promoted cell viability at concentrations of 125, 250 and 500 μg/ml. noni root ethanolic extract has neuroprotective effect against oxidative stress in SK-N-SH cells induced by H 2O 2, suggesting that noni root ethanolic extract is a highly promising agent in the prevention of Alzheimer's disease. 135 The 44 th Congress on Science and Technology of Thailand (STT 44)

142 B1_003_PF: ANTIOXIDANT ACTIVITY AND CYTOTOXICITY EFFECT OF MUSHROOM EXTRACTS Tassanee Phermthai 2, Nunticha Somrit and Jongrak Attarat 1, * 1 Department of Biochemistry, Faculty of Medical Science, NU, Thailand 1,* Center of Excellence in Fungal Research (CEFR), NU, Thailand 2 Department of Obstetric and Gynecology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10400, Thailand * rakae19@hotmail.com Abstract: Fruiting body of Lentinus squarrosulus and Volvariella volvacea were extracted with hot water extraction and ethanol precipitation. β-glucan and total phenolic compound content of L. squarrosulus extract were 0.34 mg/ml, 8.55 mg GAE /g sample, respectively. For β-glucan content and total phenolic compound of V. volvacea extract were 0.66 mg/ml, mg GAE/g sample, respectively. In vitro antioxidant activity assay, percentage of DPPH inhibition by L. squarrosulus extract showed only 33% while it was 43% by V. volvacea extract. FRAP assay was also used to check antioxidant activity, V. volvacea extract also displayed more antioxidant activity than L. squarrosulus extract. Viability of HepG2 cell was 40% and 55% after treatment with V. volvacea and L. squarrosulus extracts, respectively. From this study, cytotoxicity effect of V. volvacea was corresponding with antioxidant activity and β-glucan content in the mushroom extract. V. volvacea extract might be useful for cancer treatment in further study. Keywords: Antioxidant activity, Lentinus squarrosulus extract, Volvariella volvacea extract, HepG2 cell 136 Science and Technology in the Disruptive Era

143 B1_004_PF: EFFECTS OF SOLVENTS AND PEANUT SKIN ON ANTIOXIDANT CAPACITY AND PHENOLIC CONTENT OF RICE BRAN OILS Preeyaporn Phonalwut, Prasan Swatsitang* Department of Biochemistry, Faculty of Science, Khon Kaen University, Thailand * Abstract: Rice bran oil is a natural source of antioxidants such as tocopherols, tocotrienols and -oryzanol, which make it different from other vegetable oils. This study aimed to investigate the antioxidant capacity of different brands of rice bran oil and crude rice bran oil according to types of solvent used for extraction and also determine the effect of peanut skin on its antioxidant capacity and phenolic content. The results showed that methanol extract of rice bran oil no. 2 (RBO2) gave the greatest values of total phenolic and flavonoid contents, which were and g CE/g oil, respectively. Methanol extract of crude rice bran oil (RBO5) gave the greatest yeild of flavanol (33.35 ± 1.83 µg CE/g oil). The greatest antioxidant capacity (by DPPH assay) was found in the hexane+methanol extract of RBO2 by giving the trolox equivalent value of ± 3.54 µg TE/g oil. The ethanol extract of RBO2 showed the greatest antioxidant capacity in ABTS assays (Trolox equivalent = ± 2.08 µg TE/g oil). The methanol extract of RBO2 showed the greatest antioxidant capacity in FRAP assay (Trolox equivalent = ± 2.68µg TE/g oil). Methanol extract of RBO1 with peanut skin showed greater total phenolic, flavonoid and flavanol contents by giving the values of 6, ± , 3, ± and 1, ± µg CE/g oil, respectively. This extract also showed greater antioxidant capacity than those of the other rice bran oil extracts by giving the value of trolox equivalent = 1, ± 67.64, 13,111.8 ± and 3, ± µg TE/g oil in DPPH, ABTS and FRAP assays, respectively. The content of oryzanol appeared to affect the antioxidant capacity of rice bran oil. Peanut skin could increase phenolic content and antioxidant capacity of rice bran oil. 137 The 44 th Congress on Science and Technology of Thailand (STT 44)

144 B1_005_PA: ANTIOXIDANT ACTIVITY OF CATIONIC AMPHIPATHIC PEPTIDE MODIFIED FROM IL15 PEPTIDE FROM SIAMESE CROCODILE HEMOGLOBIN HYDROLYSATE Nisachon Jangpromma 1,2, *, Santi Phosri 3, Boonpob Nowichai 2,4, Anupong Joompang 2,4 and Sompong Klaynongsruang 2,4 1 Department of Integrated Science, Forensic Science Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 3 Office of Education, Faculty of Engineering, Burapha University, Chonburi 20131, Thailand 4 Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand * nisaja@kku.ac.th Abstract: Known antimicrobial and antioxidant peptide IL15 (IIHNEKVQAHGKKVL) from Crocodylus siamensis hemoglobin hydrolysate was used as a template for the design of new cationic amphipathic peptide IL-K (IKHWKKVWKHWKKKL). Antioxidant activity was measured according to the 2,2 -azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radicals scavenging method. The results indicated that IL-K exhibited higher antioxidant than IL15. The average percentage of radicals scavenging by 64% was observed in IL-K, while the average efficiency in radicals scavenging of IL15 was 16%. To determine the antioxidant activity of both peptides on HaCaT human keratinocytes cell, hydrogen peroxide (H 2O 2) was selected as the oxidant. The 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide solution (MTT) assay demonstrated that the oxidative damage induced by H 2O 2 to HaCaT cells led to a significant reduction of cell growth (21% cell viability). A protective effect against this damage was observed upon co-culture of the cells with IL15 and also IL-K. The HaCaT cell viability of IL15 was determined as 29% while IL-K showed cell viability as 35%, respectively. It is therefore concluded that IL-K that modified from Siamese crocodile hemoglobin possesses a great potential to scavenge free radical in cells. Acknowledgments: This research was supported by National Natural Science Foundation of China (NSFC)-Thailand Research Fund (TRF) Joint Research Fund (DBG ), Thailand Research Fund (TRF) (MRG ) and Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Thailand. 138 Science and Technology in the Disruptive Era

145 B1_006_PF: OVEREXPRESSION AND PURIFICATION OF FICOLIN IN THE BLACK TIGER SHRIMP, Penaeus monodon Prawit Oangkhana, 1 Ratree Wongpanya 2, * 1 Interdisciplinary graduate program in genetic engineering, Kasetsart University, Bangkok, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, * fscirtw@ku.ac.th Abstract: Ficolin is a carbohydrate binding proteins that plays important role in recognizing and eliminating pathogens in the innate immunity of shrimp. In the present study, we constructed a recombinant plasmid for gene expression, and purified the recombinant protein ficolin from the black tiger shrimp, Penaeus monodon (rpmflp). The rpmflp was expressed in E. coli BL21 star (DE3) codon plus using pet43.1a-flp, at 25ºC for 4 hours. This condition was used for large scale expression, and the protein was purified using Ni-NTA affinity. Hydrophobic interaction and ion exchange chromatography were also carried out. 139 The 44 th Congress on Science and Technology of Thailand (STT 44)

146 B1_007_PA: EFFECT OF CHEMICAL CROSS-LINKING IN CROCODILE (Crocodylus siamensis) AND HUMAN HEMOGLOBIN Kanchana Rotcharoen 1,2, Napaporn Roamcharern 1,2, Wisarut Payoungkiattikun 2, Yosapong Temsiripong 3, Nisachon Jangpromma 2, Sompong Klaynongsruang 1,2* 1 Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 3 Sriracha Moda Co., Ltd., Sriracha, Chonburi 20110, Thailand * somkly@kku.ac.th Abstract: Inadequate of blood supply for human blood transfusion may be happening in the near future depending on exponential growing of human population. However, donated blood has contained a lot of limitations. Hence, development of blood substitution or hemoglobin-based oxygen carriers (HBOCs) from non-human hemoglobin is an interesting alternative route. This study aims to develop both of cross-linking chemicals and reaction processes using two chemicals, namely glutaraldehyde (GTA) and Bis (3,5-dibromosalicyl) fumarate (BDSF or DBBF). GTA is non-specific cross-linking agent which provide both intra- and inter-molecular cross-linking on hemoglobin (Hb). While, BDSF is specific cross-linking agent which provide only specific intra-molecular cross-linking property on human hemoglobin (hhb). Cross-linked Hb could help to reduce the dissociation of tetrameric form and prevent toxicity against normal cells comparing to that of unmodified cell-free Hb. The result demonstrated that GTA-crosslinked crocodile hemoglobin (CL-cHb) and GTA-cross-linked human hemoglobin (CL-hHb) revealed inter- and intra-molecular cross-linking behaviors. Moreover, molecular docking analysis revealed that GTA demonstrated the intra-molecular cross-linking property on α 1 and α 2 subunit, Lys128 (α 1)-Lys140 (α 2), Lys140 (α 1)-Lys128 (α 2), and Lys100 (α 1)-Lys100 (α 2), better than that of other subunits. In term of BDSF cross-linking reaction, only human hemoglobin could form cross-linked dimer within 3.5 h incubation time. While, oxygen affinity characteristic of GTA CL-cHb was similar to that of native hhb. Thus, it is important to note that GTA CL-cHb may be used as a modified blood substitute for human blood transfusion in the future. 140 Science and Technology in the Disruptive Era

147 B1_008_PF: DEVELOPMENT OF DNA NANOBIOSENSORS TARGETING HER2-POSITIVE CANCER CELLS Chanida Wootthichairangsan, 1 Anuttara Udomprasert, 2, * Thaned Kangsamaksin 1, * 1 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand 2 Department of Biochemistry, Faculty of Science, Burapha University, Chonburi, Thailand * anuttara@buu.ac.th, thaned.kan@mahidol.ac.th Abstract: Early detection is difficult for some cancers including nasopharyngeal carcinoma (NPC) because the patients usually show signs/symptoms in advanced stages. Thus, researchers have been trying to develop powerful tools to detect cancer cells effectively in early stage. Quantum dots (QDs) have been used as molecular probes with high potential in many previous studies; however, their cytotoxicity is still concerned. In this study, we proposed to develop a DNA-based nanobiosensor to detect cancer cells. We planned to construct DNA nanosphere using scaffold DNA origami technique and encapsulate QDs into DNA nanostructure. To enhance specificity of our nanobiosensor to NPC cells, HER2-specific DNA aptamer was modified onto its surface. After annealing process, DNA nanosphere was characterized via agarose gel electrophoresis and atomic force microscopy (AFM). The results showed that the DNA nanostructure was successfully constructed with a spherical shape and a diameter of roughly nm. In contrast to QDs, the cytotoxicity assay indicated that DNA nanosphere was not toxic to cultured cells when using high concentrations. Moreover, the expression of HER2 proteins in both NPC cell lines and the specific binding of HER2 aptamer and NPC cell lines were determined using Western blot analysis and fluorescence microscopy, respectively. Our work demonstrated the possibility of our DNA nanobiosensor to be used as an effective tool for cancer theranostic applications. 141 The 44 th Congress on Science and Technology of Thailand (STT 44)

148 B1_009_PF: ANTICANCER ACTIVITY OF ACTIVE COMPOUNDS FROM THAI MEDICINAL PLANTS ON A HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE Rattanavinan Hanchaina, Tavan Janvilisri, Tuangporn Suthiphongchai* Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand * tuangporn.sut@mahidol.ac.th Abstract: Nasopharyngeal carcinoma (NPC) is an epithelial malignancy of the nasopharynx and is one of the most common tumors in China and Southeast Asia. Current therapies involving chemo- and radiotherapy are ineffective, costly and many serious side effects can arise. Therefore, new drugs or therapy that can replace the existing current therapies are urgently needed. The purpose of this study was to screen four compounds from Thai medicinal plants for the anticancer activity on an NPC cell line, TW-01 cells. Four compounds, namely eugenol, geraniol, citral and galangin were prepared (0-500 M) and were screened for their cytotoxicity on the TW-01 cells using methylthiazoldiphenyl tetrazolium assay. Our results demonstrated that citral exhibited the most potent activity and reduced TW-01 cell viability in time- and dose-dependent manners. The IC 50 values of citral on TW-01 cells at 24, 48 and 72 hours were 84 3, 46 3 and M, respectively. Moreover, the results from flow cytometry revealed that citral affected cell cytotoxicity by the induction of cell necrosis. The findings herein provides the information of citral that will be useful for the development of the alternative chemotherapeutic agent from Thai medicinal plants for NPC patients. 142 Science and Technology in the Disruptive Era

149 B1_010_OF: EXOPOLYSACCHARIDE PRODUCED BY Bacillus subtilis LY 7/16 ISOLATED FROM THAI TRADITIONAL FERMENTED SOYBEAN AND ITS BIOACTIVITIES Vongsathorn Ngampuak 1, Supichaya Laothong 1, Chakrit Tachaapaikoon 1,2, Patthra Pason 1,2, Khanok Ratanakhanokchai 1 and Rattiya Waeonukul 1,2, * 1 School of Bioresources and Technology, King Mongkut s University of Technology Thonburi (KMUTT), Bangkok, Thailand, Pilot Plant Development and Training Institute (PDTI), King Mongkut s University of Technology Thonburi (KMUTT), Bangkok, Thailand, * rattiya.wae@kmutt.ac.th Abstract: A strain of Bacillus subtilis LY 7/16 with high exopolysaccharide (EPS) production ability was isolated from Thai traditional fermented soybean and identified based on morphological characteristic and 16S rrna sequences analysis. The EPS composition was analyzed by acid hydrolysis and performed on high-performance liquid chromatography. The results revealed that it was levan polysaccharides comprising of only fructose monomers. Moreover, the levan from LY 7/16 displayed in vitro bioactivities such as antioxidant activity in term of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity with 55.87% at 3.5 mg ml-1 and immune stimulation of nitric oxide production from murine macrophage RAW cells with 100% at 0.35 mg ml-1. Additionally, it showed as great prebiotic properties with positive effect on probiotics growth as well. Therefore, the levan-type EPS with in vitro bioactivities from B. subtilis strain LY 7/16 is useful for several applications in food, feed, cosmetic and medicine areas. 143 The 44 th Congress on Science and Technology of Thailand (STT 44)

150 B1_011_PA: LIPASE CATALYZED ESTERIFICATION OF 3,4,5-TRIHYDROXYCINNAMIC ACID TO INCREASE ITS BIOLOGICAL ACTIVITIES Pirom Chenprakhon, 1* Wachirawit Chinantuya 2, Muttakeen Che-leah 1, Pimchai Chaiyen 3 1 Institute for Innovative Learning, Mahidol University, Thailand 2 Department of Biochemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 3 Department of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology, Wangchan, Rayong 21210, Thailand * Pirom.che@mahidoil.ac.th Abstract: 3,4,5-trihydroxycinnamic acid and its ester derivatives have a variety of biological activities such as inhibitory and cytotoxic effects against cancer cell, anti-inflammatory and antimicrobial activities, etc. Therefore, the compounds can be potentially used as bioactive ingredients in cosmetic and food industries. In this study, ester derivatives of 3,4,5- trihydroxycinnamic acid were synthesized using lipase (Novozyme 435) catalyzing esterification. Novozyme 435 catalyzed the reaction of 3,4,5-trihydroxycinnamic acid with various types of alcohol including methanol, ethanol, butanol, octanol and phenethyl alcohol in binary system of t-butanol and isooctane (1:16). New ester products were analyzed and identified by LC/MS. At optimal condition, the conversion reached 98.9 %, 100%, 96.9%, 96.8% and 63.2% for the synthesis of 3,4,5-trihydroxycinnamic acid methyl ester, 3,4,5-trihydroxycinnamic acid ethyl ester, 3,4,5-trihydroxycinnamic acid butyl ester, 3,4,5-trihydroxycinnamic acid octanyl ester and 3,4,5-trihydroxycinnamic acid phenapthyl ester, respectively. The 3,4,5- trihydroxycinnamic acid methyl ester and 3,4,5-trihydroxycinnamic acid ethyl ester were purified from reaction mixtures using Sep-Pak C18 cartridges. The large-scale purification of products will be established and the biological activities of products will be tested. 144 Science and Technology in the Disruptive Era

151 B1_012_PA: Identification of MYB transcription factors controlling the biosynthesis of isoflavonoids in Pueraria mirifica Kittiya Tantisuwanichkul 1, Nithiwat Suntichaikamolkul 1, Supaart Sirikantaramas 1,2 * 1 Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok Thailand 2 Natural Product Biotechnology Research Unit, Chulalongkorn University, Bangkok Thailand * supaart.s@chula.ac.th Abstract: Pueraria mirifica or White Kwao Krua, a legumimous plant, contains many isoflavonoid compounds which act as a phytoestrogen showing high estrogenic-activity. These compounds are biosynthesized from the phenylpropanoid pathway and their biosyntheses are regulated by MYB transcription factors in several plants. However, MYB transcription factors controlling isoflavonoid biosynthesis in this plant have not been reported. In this study, we identified MYB transcription factors in P. mirifica from its transcriptome database. We found eight MYB transcription factors that might be involved in P. mirifica isoflavonoid biosynthesis. Quantitative real-time PCR analysis showed the high expression levels of Pm_uni13739 and Pm_uni37601 in P. mirifica tuber root cortex where the high amount of puerarin, an isoflavone, was found. These MYB transcription factors, in which their gene expression levels are correlated with metabolite accumulation, might be good candidates for further functional characterization. 145 The 44 th Congress on Science and Technology of Thailand (STT 44)

152 B1_013_PA: INVESTIGATION THE THERMOSTABILITY OF AMYLOMALTASE FROM Streptococcus agalactiae BY SITE-DIRECTED MUTAGENESIS AT C447 Suthipapun Tumhom, Kittikhun Wangkanont, Piamsook Pongsawasdi * Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand * piamsook.p@chula.ac.th Abstract: Amylomaltase (AM) catalyzes α-1,4 glucosyl transfer of the inter- and intra-molecular transglucosylation reaction to yield linear or cyclic oligosaccharide products, respectively. This work aims to investigate the amino acid involved in thermal property of AM. A novel AM from the mesophilic Streptococcus agalactiae (SaAM) with a low amino acid sequence identity (40%) compared to the thermostable AM from Thermus sp was used in this study. Cysteine 447 of SaAM was selected as the target for mutation. pet-28a carrying wild-type (WT) SaAM gene was used as template, site-directed mutagenesis was performed to substitute C447 by alanine (A), proline (P) and serine (S). The mutated genes were confirmed by DNA sequencing and transformed into E. coli Tuner TM (DE3) cells for protein expression. The mutated enzymes showed an overexpressed protein band of 57 kda similar to WT. The specific disproportionation activities of the purified C447A, C447P and C447S- SaAMs were higher than that of WT (2.5, 1.4 and 2.4 folds, respectively). The optimum temperature and ph of mutants was not changed. Thermal stability at C of C447A and S mutants was significantly increased from 1 hour to 2 hours when compared to WT. The further enzyme characterization will be determined by biophysical methods. 146 Science and Technology in the Disruptive Era

153 B1_014_PA: IDENTIFICATION OF DAMAGE-ASSOCIATED MOLECULAR PATTERNS (DAMPs) FROM SHRIMP Litopenaeus vannamei Supitcha Wanvimonsuk, 1 Phattarunda Jaree, 2 Taro Kawai, 3 Kunlaya Somboonwiwat 1 1 Center of Excellence for Molecular Biology and Genomics of Shrimp, Departmemt of Biochemistry, Faculty of science, Chulalongkorn University, Bangkok 10330, Thailand 2 Institute of Molecular Bioscience, Mahidol University, Nakhon Pathom 73170, Thailand 3 Laboratory of Molecular Immunobiology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan * Kunlaya.S@chula.ac.th Abstract: Damage-associated molecular patterns (DAMPs) are molecules that act as endogenous danger signals to promote immune response. These signals are released by heat stress. Several reports have shown that non-lethal heat shock (NLHS) can enhance protection against bacterial infection in aquatic animals. However, the molecular mechanisms underlying the EMS resistance in NLHS-treated shrimp is not known. So, this research aims to identify DAMPs that can induce innate immune response against pathogens after heat treatment in shrimp Litopenaeus vannamei. Differential gene expression of the candidate DAMPs genes in shrimp s hemocytes was performed by qrt-pcr to examine the effect of NLHS. The result showed that the expression of heat shock protein 70, heat shock protein 90, actin, peroxiredoxin and high-mobility-group box b were significantly increased after heat treatment. So, these proteins might be activated through NLHS treatment. 147 The 44 th Congress on Science and Technology of Thailand (STT 44)

154 B1_015_PA: PHYSICAL CHROMOSOME MAP USING A PRIMED IN SITU HYBRIDIZATION TECHNIQUE FOR CULTIVATED STRAWBERRY (Fragaria ananassa) Kornlawat Tantivit, 1, * Yanagi Tomohiro, 1 Preeda Nathawat, 2 Sachiko Isobe 3 1 Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kita, Kagawa Japan 2 Faculty of Agricultural Production, Maejo University, 63 Sansai-Phrao Road, Nongharn, Sansai, Chiang Mai Thailand 3 Kazusa DNA Research Institute, Yana, Kisarazu, Chiba Japan * kornlawat@gmail.com Abstract: The allo-octoploidy of cultivated strawberry (Fragaria ananassa) made it difficult to conduct a theoretical genetic analysis based on the Mendel s law of inheritance. It has been reported to have four sub-genomes originated with the diploid Fragaria vesca, one with the diploid Fragaria iinumae, and two with an unknown ancestor close to F. iinumae. To elucidate the inheritance mode of F. ananassa, Isobe et al. (2013) developed a large number of DNA markers and constructed a genetic linkage map. Therefore, physical chromosome mapping using DNA markers is required to evaluate the accuracy of the linkage groups (LGs). Primed in situ hybridization (PRINS) is one of the cytogenetic techniques which can provide the location of DNA sequences on the physical chromosome map with high resolution. This technique has been a powerful tool for verifying the accuracy of linkage map in cultivated strawberry. In this study, the primers from both ends of each LG were labeled on chromosomes by PRINS hybridization. Our results clearly confirmed that fourteen groups including 1A (Fig.1), 1C, 1D, 2A, 2C, 2D, 4A, 4C, 5A, 5B, 5C, 6A, 6D, and 7A were created correctly. Figure 1. Example image of the physical chromosome mapping to confirm the accuracy of linkage group 1A (left) and enlarged images of the chromosomes which had red and green signals (right). Signals were marked with arrowheads. 148 Science and Technology in the Disruptive Era

155 B1_016_PA: IDENTIFICATION OF C- and O-GLYCOSYLTRANSFERASES INVOLVED IN PHYTOESTROGEN BIOSYNTHESIS IN Pueraria mirifica Nithiwat Suntichaikamolkul 1, Sornkanok Vimolmangkang 2, Wanchai De-Eknamkul 2, Supaart Sirikantaramas 3, * 1 Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3 Omics Sciences and Bioinformatics Center, Chulalongkorn University, Bangkok 10330, Thailand * Supaart.S@chula.ac.th Abstract: Pueraria mirifica, a Thai medicinal plant, has long been used in Thai traditional medicine because of numerous phytoestrogens originated. Phytoestrogen glycosides (such as daidzin, genistin, genistein 8-C-glucoside, puerarin, and mirificin) are the largest portion of phytoestrogens found in this plant, promising as the chemical marker for pharmaceutical industry. Herein, deep mining of de novo assembled-transcriptome of P. mirifica generated from Illumina platform led to the identification of 41 unigenes encoding UDP-glycosyltransferase. We performed phylogenetic analysis to briefly categorize them into a total of five clusters based on characterized proteins in the public database. Furthermore, we also employed LC-MS/MS platform to evaluate the metabolite profile across four tissues of P. mirifica; young leaves, mature leaves, cortex-excised tuber, and tuber cortex. Based on the positive correlation of differential expression of genes (DEGs) generated by Illumina sequencing and metabolite profiles across those four tissues, we obtained four and seven putative genes involved in C-glycosylation and O-glycosylation, respectively. To confirm their functions, these candidate genes will be cloned and characterized both in vitro and in planta. 149 The 44 th Congress on Science and Technology of Thailand (STT 44)

156 B1_017_PF: CHARACTERIZATION OF MELATONIN-NOTCH SIGNALING AXIS IN NEUROGENESIS OF HUMAN PLURIPOTENT STEM CELLS Nut Phueakphud 1, Nopporn Jongkamonwiwat 2, Tulyapruek Tawonsawatruk 3, Narisorn Kitiyanant 4, Piyarat Govitrapong 5, Patompon Wongtrakoongate 1, * 1 Department of Biochemistry, Faculty of Science, Mahidol University, Thailand 2 Feinberg School of Medicine, Northwestern University, Chicago, USA 3 Department of Orthopedics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Thailand 4 Research center of Neuroscience, Institute of Molecular Biosciences, Mahidol University, Thailand 5 Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok, Thailand * p.wongtrakoongate@gmail.com Abstract: Melatonin (N-acetyl-5-methoxytriptamine) is a pineal gland secreted hormone which controls various physiological processes including circadian rhythms and seasonal change response. Moreover, melatonin has been reported to be necessary for pregnancy and embryo development. In mouse, a role of melatonin in promoting neurogenesis by inhibition of Notch signaling has been elucidated. However, whether melatonin affects neurogenesis in human has been elusive. Therefore, this study aims to determine whether melatonin regulates differentiation of human pluripotent stem cells toward neural derivatives via modulation of Notch signaling pathway. Human pluripotent stem cell line NTERA2 was treated with neural lineage differentiation inducer retinoic acid with and without melatonin. RT-qPCR analysis reveals that melatonin attenuates expression of PAX6 and TLX, which encode key transcription factors of neural stem cells. In addition, melatonin represses expression of MAP2 and GLAST, which represent markers of mature neuronal and glial cells, respectively. However, immunofluorescent staining shows that melatonin decreases number of neuronal cells but increases number of glial cells. The direct Notch target genes HES1, HEY1 and HEY2 are also down-regulated by melatonin. Combined treatment of melatonin and melatonin receptor antagonist luzindole could rescue HES1 expression. Collectively, the results indicate that melatonin inhibits Notch signaling through melatonin receptor mediated pathway. Nonetheless, how melatonin inhibits neuronal differentiation of human pluripotent stem cells is still needed to be elucidated. 150 Science and Technology in the Disruptive Era

157 B1_018_PA: FUNCTIONAL CHARACTERIZATION OF VAGO5 GENE IN THE Litopenaeus vannamei IMMUNE SYSTEM UPON Vibrio parahaemolyticus AHPND INFECTION Hafeeza Sakor, 1 Pakpoom Boonchuen, 1 Kunlaya Somboonwiwat* 1 1 Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of science, Chulalongkorn University, Phayathai Rd, Wangmai, Pathumwan, Bangkok 10330, Thailand. * Kunlaya.S@chula.ac.th Abstract: Litopenaeus vannamei is the most popular shrimp species in shrimp aquaculture industry. Acute hepatopancreatic necrosis disease (AHPND) of shrimps is an important disease. Since 2009, AHPND has caused serious drops in shrimp production. The outbreak disease is caused by Vibrio parahaemolyticus (VP AHPND) that can produce PirAB toxin (PirAB vp ). Previously, suppression subtractive hybridization (SSH) was used to identify the differentially expressed genes from shrimp hemocytes after challenged with VP AHPND. The qrt-pcr showed that the Vago5 gene expression was significantly up-regulated after VP AHPND infection. In this study, the function of Vago5 gene in shrimp immune system in response to VP AHPND infection was characterized using RNA interference technique. The recombinant plasmid encoding for dsrna specific to Vago5 gene (dsrna-vago5) was constructed and transformed into E. coli HT115. After dsrna-vago5 overexpression, the dsrna-vago5 was purified and used for RNAi. Finally, the cumulative mortality and the bacterial counting from stomach and liver tissues at different time points dsrna challenged were observed. The results showed that cumulative mortality of dsrna-vago5 injected shrimps was significantly increased and the number of bacteria in the stomach and liver tissues was higher than control group. Taken together, it can be concluded that the Vago5 gene is an important gene of shrimp immunity against VP AHPND infection. 151 The 44 th Congress on Science and Technology of Thailand (STT 44)

158 B1_019_PF: ESTABLISHMENT OF STABLE INTEGRIN Β4 (ITGB4) CONDITIONAL KNOCKDOWN CHOLANGIOCARCINOMA CELL LINE Hnin Htet Kyaw, Kittiya Islam, Tuangporn Suthiphongchai * Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand * address: tuangporns@yahoo.com Abstract: Cholangiocarcinoma (CCA) or bile duct cancer is the cancer originating from the epithelial cells of bile duct which drains the bile from the liver to the small intestine. The CCA incidence is high in Thailand, especially in the Northeastern part, which is associated with liver fluke (Opisthorchis viverrini) infestation. The prognosis of CCA is poor and it results in high mortality and imposes a significant burden to the society. Integrins are heterodimeric transmembrane proteins which consist of α and β subunits. Integrin α6β4 is expressed primarily in the basal surface of epithelial cells and mediates the formation of stable structure known as hemidesmosome, which serves as laminin receptor to anchor epithelium to the basement membrane. In contrast to this function, integrin α6β4 is involved in the migration of carcinoma cells which in turn promotes cancer cell invasiveness and metastasis. Integrin β4 (ITGB4) overexpression has been reported in several cancers and is associated with cancer aggressiveness and poor prognosis. Here we showed that three out of five CCA cell lines overexpressed ITGB4 protein when compared to non-cancerous cholangiocyte. We then aim to investigate the roles of ITGB4 in CCA cell line by knocking down of ITGB4 in ITGB4-overexpressing CCA cell line, HuCCA-1. A Tet-pLKO-puro-shITGB4 plasmid was successfully constructed and its sequence was confirmed. This lentivirus containing shitgb4 was transduced into HuCCA-1 cell and the stable cell line was selected by puromycin treatment. The selected cells expressed tet repressor mrna (as shown by RT-qPCR) indicating the succeed in the establishment of stably transduced cells carrying the lentiviral plasmid. Silencing of ITGB4 in this cell will be confirmed after doxycycline treatment. Keywords: Cholangiocarcinoma; ITGB4; knockdown 152 Science and Technology in the Disruptive Era

159 SESSION B: BIOLOGICAL SCIENCE (B2: MICROBIOLOGY) 153 The 44 th Congress on Science and Technology of Thailand (STT 44)

160 B2_001_PF: THE EFFICACY OF HYDROGEN PEROXIDE, ETHYL ALCOHOL AND BIOACTIVE COMPOUNDS PRODUCED FROM Streptomyces sp. KB1 TISTR2304 AS EFFECTIVE DISINFECTANTS AGAINST MICROORGANISMS Monthon Lertcanawanichakul, 1,2, * Kittisak Chawawisit 1,2 1 School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, Thailand. 2 The Research Unit of Natural Products Utilization, Walailak University, Nakhon Si Thammarat 80161, Thailand. * lmonthon55@gmail.com Abstract: A host environment cleaning using disinfectants is the most effective way to prevent the pathogen s transmission. This study compared the activity among of commercial liquid disinfectants [3% hydrogen peroxide (HP), and 70% ethyl alcohol (EA)] and bioactive- compounds (BCs) from Streptomyces sp. KB1 on indicating microorganisms: Bacillus subtilis ATCC 6633 as representative of spores; Staphylococcus aureus TISTR 517 as representative of gram-positive bacteria, Escherichia coli TISTR 887, Pseudomonas aeruginosa TISTR 1467, and extended spectrum beta-lactamase (ESBL)-Klebsiella pneumoniae 342 as representative of gram-negative bacteria; Candida albicans TISTR 5779 as representative of yeast. Results showed that B. subtilis spores is resistant to 70% EA and BCs from strain KB1- which are biocidal to TISTR strains and clinical isolate ESBL - in the free floating microorganism (suspension test). On the other hand, this resistance was not observed with 3% HP. Thus, 3% HP showed more promise for disinfecting microorganisms than that 70% EA and BCs. However, BCs did show the antimicrobial activities equal as 70% EA. The results might be implied that the using of BCs from selected bacterial strain as effective disinfectant against microorganism, especially vegetative cell, is a sustainable application in nearly future, to reduce using the chemical substances and zero wastes. 154 Science and Technology in the Disruptive Era

161 B2_002_PA: ANTIMICROBIAL EFFECT OF NOVEL SYNTHETIC PEPTIDE DESIGNED FROM Crocodylus siamensis HEMOGLOBIN HYDROLYSATE ON Escherichia coli Sirinthip Sosiangdi, 1,2 Sompong Klaynongsruang, 1,2 Nisachon Jangpromma 2,3, * 1 Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 3 Department of Integrated Science, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand * nisaja@kku.ac.th Abstract: The hydrolyzed Crocodylus siamensis hemoglobin peptide namely IL15 (IIHNEKVQAHGKKVL) was previously described for their antibacterial activity. However, this peptide had short half-life in mammalian reticulocytes (in vitro) when estimated by ProtParam tool. Accordingly, the present research was aimed to design novel antimicrobial peptide by substitution of lysine (K) residue in IL15 to extend the cationic net charge from +2 to +6. Whereas tryptophan (W) residue was chosen to increase the percentage of hydrophobicity from 40% to 46%. The antimicrobial activity was evaluated against Gram-negative bacterium using broth microdilution assay and plate count methods. IL15-modified exhibited strong antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC, that percentage inhibition more than 90) value of 4 μg/ml and minimum bactericidal concentration (MBC, that killed bacteria) value of 6 μg/ml. Furthermore, the kinetic killing analysis results showed that IL15-modified could destroy E. coli within one hour at MIC value. Mode of action of this peptide was further elucidated using scanning electron microscope (SEM) technique. The results revealed that the bacterial cell membrane is dramatic destruction as a dose-dependent manner. This study is expected that the IL15- modified peptide can develop as the next generation of antibiotics and might solve antimicrobial resistant problem in the future. 155 The 44 th Congress on Science and Technology of Thailand (STT 44)

162 B2_003_PA: PLASMA FROM CROCODILE (Crocodylus siamensis) REVEALING ANTIMICROBIAL ACTIVITY AGAINST P.seudomonas aeruginosa Thanawan Srilert 1,2, Sompong Klaynongsruang 1,2 and Nisachon Jangpromma 2,3,*. 1 Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), 3 Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand Department of Integrated Science, Forensic Science Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand * nisaja@kku.ac.th Abstract: Pseudomonas aeruginosa is a biofilm formation type that can cause of drug resistance. Consequently, the discovery of natural molecules which effectively inhibit bacterial cell membrane and also safe therapies is urgently needed. Crocodylus siamensis plasma (C-plasma) has been investigated for their antimicrobial activity. However, the inhibition of biofilm formation bacteria has not been reported. Thus the objectives of this study were to investigate antibacterial activity of C-plasma against P.aeruginosa ATCC and also observe for their stability under ultraviolet (UV) light. The results from broth micro-dilution assay indicated that C-plasma effectively inhibited P.aeruginosa approximately 2-fold higher than human plasma (H-plasma). Indeed, the efficiency in inhibition the P.aeruginosa growth of C-plasma was averaged by 80%, while the average percentage of inhibition by 40% was obtained in H-plasma. The stability of C-plasma on P.aeruginosa growth inhibition was performed under UVA and UVC treatment. The results showed that C-plasma after treated with UV (1,000 µg/ml) slightly decrease in their percentage inhibition on P.aeruginosa of about 90%, which was a little lower than untreated C-plasma (98%). Hence, the results of this research directly indicated that the strong antimicrobial activity on P.aeruginosa of C-plasma could provide potential candidates for developing therapeutic agents against biofilm formation bacteria. 156 Science and Technology in the Disruptive Era

163 B2_004_PA: SCREENING AND IDENTIFICATION OF LIPOLYTIC PRODUCING Bacillus AND Staphylococcus STRAINS FROM SHRIMP PASTE (KA-PI) Rungsima Daroonpunt, 1 Somboon Tanasupawat 1 * 1 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand * d_rungsima@outlook.com Abstract: Twelve bacterial strains were isolated from shrimp paste (Ka-pi) by the standard dilution technique using nutrient broth (NB) medium supplemented 5% NaCl. On the basis of their phenotypic characteristics and 16S rrna gene sequence analyses ( % sequence similarity), six rod-shaped isolates were belonged to the genus Bacillus and 6 coccal isolates were Staphylococcus. Strain KP1-09 was identified as Bacillus tequilensis, KP1-04 and KP2-03 as B. infantis, KP1-14 as B. flexus KP2-17 as B. paramycoides and KP1-10 was Bacillus strain. Strains KP3-03, KP4-02, KP4-08, KP5-02, KP5-03 and KP5-07 were identified as Staphylococcus cohnii subsp. urealyticus. The results of lipase activity of strains in modified NB and in modified NB medium supplemented with 1% (v/v) Tween 20 or Tween 80 using para-nitrophenyl butyrate (C4) as a substrate ranged from 4.41± ±0.80 Unit/mL. The strain B. flexus KP1-14 cultivated in modified NB medium showed the highest lipase activity. While the strains KP1-10, KP1-09, KP4-02, KP5-02 and KP5-07 cultivated in modified NB medium supplementary with 1% (v/v) Tween 80, showed the lipase activity ranged from 0.32± ±0.24 Unit/mL when p-nitrophenyl palmitate (C16) was used as a substrate, and S. cohnii subsp. urealyticus strain KP5-02 showed the highest lipase activity. 157 The 44 th Congress on Science and Technology of Thailand (STT 44)

164 B2_005_PF: DECREASED EXPRESSION OF BID PRO-APOPTOTIC MOLECULE IN HEPATITIS B VIRUS INFECTED-LIVER CANCER CELLS AND INTERACTION WITH AUTOPHAGY-RELATED PROTEIN Jiaranai Peantum 1, Areerat Kunanopparat 2, Nattiya Hirankarn 2, Pisit Tangkijvanich 3, Ingorn Kimkong 1,4,* 1 Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand 2 Center of Excellence in Immunology and Immune Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 3 Research Unit of Hepatitis and Liver Cancer, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand 4 Center for Advanced Studies in Tropical Natural Resources, National Research University Kasetsart University, Bangkok, Thailand * fsciiok@ku.ac.th: jiaranaihk@gmail.com Abstract: Hepatitis B virus (HBV) infection is a global public health problem and a major cause of acute and chronic hepatitis that can be progressed to cirrhosis and hepatocellular carcinoma (HCC). In cancer, autophagy and apoptosis are important pathways: autophagy defects can be found in cancer and/or apoptosis acts as a tumor suppressor pathway. Previous studies, HCC was showed an imbalance between the pro- and anti-apoptosis of Bcl-2 family members, particularly in BH3-interacting domain death agonist protein (BID). Therefore, we designed the experiments to investigate BID expression profile both in gene and protein levels. Under starvation condition, our results showed that BID gene level was significantly decreased in HepG and HepG2 cells when estimated to normal liver cell respectively, and corresponding with our protein level results. Furthermore, to consider the ability of dual-function, we additionally observed BID protein expression in the stable shatg16l1 transfected cells. This result indicated that BID is still decreased; hence BID and/or ATG16L1 may interact with other apoptotic-autophagic molecules. Taken all together, BID is a critical molecule in HBV-infected HCC cell. However, this finding needs greater understanding in the interaction between autophagy and apoptosis via Bcl-2 family and ATG molecules in HCC. 158 Science and Technology in the Disruptive Era

165 B2_006_PA: PRODUCTION OF AN OPTICALLY PURE D-LACTIC ACID BY Sporolactobacillus inulinus BK65-3 Jirabhorn Piluk 1, Sitanan Thitiprasert 1, Vasana Tolieng 1, Pajareeya Songserm 2, Nuttha Thongchul 1, * 1 Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Phayathai, Bangkok Thailand 2 Program in Biotechnology, Faculty of Science, Chulalongkorn University, Phayathai, Bangkok Thailand * Nuttha.T@chula.ac.th Abstract: D-lactic acid is commonly known as a precursor of the stereocomplex polylactic acid. With the stereocomplex structure, the mechanical and thermal properties of polylactic acid was improved. The optical purity of D-lactic acid was claimed to be responsible in color formation during the synthesis of the stereocomplex structure of polylactic acid and polymer processing. In this study, Sporolactobacillus inulinus BK65-3 was tested for the ability to produce an optically pure D-lactic acid from a simple medium with a high glucose concentration in the shaken flask culture. The fermentation was conducted at 37 C, 150 rpm, under anaerobic condition. It was found that increasing the initial glucose concentration from 120 g/l to 200 g/l resulted in the increase in final lactic acid concentration (102 g/l from 120 g/l glucose, 126 g/l from 160 g/l glucose, and 143 g/l from 200 g/l glucose). The productivity of D-lactic acid was also increased when the initial glucose concentration was increased (2.29 g/l h to 2.80 g/l h, and 3.39 g/l h, respectively). Nonetheless, the product yield dropped with the high initial glucose concentration (0.90 g/g, 0.78 g/g, and 0.76 g/g, respectively). From all 3 initial glucose concentrations studied, S. inulinus BK65-3 produced D-lactic acid with the remarkably high optical purity of 100% enantiomer excess. Keywords: D-lactic acid, Fermentation, Initial glucose, Optical purity 159 The 44 th Congress on Science and Technology of Thailand (STT 44)

166 B2_007_OF: ANTIMICROBIAL ACTIVITY OF CHITOSAN AND ITS EFFECT ON PHYSICAL AND MECHANICAL PROPERTIES OF SAGO AND CASSAVA STARCH/CLAY FILMS Muhammad Iqbal Perdana * and Montira Leelakriangsak Departement of Science, Faculty of Science and Technology, Prince of Songkla University * mu.iqbal.perdana@gmail.com Abstract: Chitosan is one of the second abundant biopolymer source after cellulose. It has been known to have antimicrobial activities against pathogenic microorganism. It also shows improving the property of starch packaging films. The aims of this research were to investigate the antimicrobial activity of different chitosan concentration (2.5 mg.ml -1, 5 mg.ml -1 and 10 mg.ml -1 ) and to investigate the physical and mechanical properties of cassava starch/clay-chitosan and sago starch/clay-chitosan biopolymer film. There was significant difference (p 0.05) in which chitosan inhibited bacterial and fungal growth. Chitosan 10 mg.ml -1 had the greatest result to inhibit Escherichia coli, Staphylococcus aureus, Bacillus sp and Pseudomonas sp with inhibition zone 3.83±0.29 mm, 7.17±1.32 mm, 5.13±3.30 mm and 4.30±0.75 mm respectively. Moreover, fungal radial growth for three days incubation of Rhizopus sp, Aspergillus niger and Penicillium sp represented lower growth of 69.80±12.64 mm, 24.93±2.70 mm and 19.73±1.20 mm respectively compared with control. Incorporating chitosan to both sago and cassava starch films increased the solubility about 33.09±1.31% and 32.5±0.19% respectively. Tensile strength of both sago and cassava film incorporated with chitosan raised of 6.19±0.98 MPa and 6.68±0.47 MPa. Elongation at break increased only on cassava about 88.22±5.74% but decreased on sago film around 30.55±2.42%. 160 Science and Technology in the Disruptive Era

167 B2_008_OA: DOUBLE-STRANDED RNA ELEMENTS OF Phytopythium cucurbitacearum Chanoknan Hattapanichaporn, Kunlanun Rungnarai, and Thanyanuch Kriangkripipat* Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand * Abstract: Para rubber tree (Hevea brasiliensis) is an important economic plant of Thailand. The major para rubber plantations with high yields are in the southern and eastern of Thailand. Tropical conditions in both areas support the plant growth and high latex yield. However, high humidity and moderate temperatures favor growth of many pathogenic fungi and fungus-like organisms. Para rubber plantations in Thailand are repeatedly affected by abnormal leaf fall and black stripe diseases caused by Phytophthora spp. In this study, we isolated an oomycete from petioles of Para rubber tree with symptoms of abnormal leaf fall from Chanthaburi province. The isolate was polyphasically identified as Phytopythium cucurbitacearum. In addition, dsrna elements were detected in the isolate. There isn't much known about oomycete viruses. Further study on biology of the virus may shed light on its potential biocontrol agent. 161 The 44 th Congress on Science and Technology of Thailand (STT 44)

168 B2_009_PF: SEROPREVALENCE OF MELIOIDOSIS IN Opisthorchis viverrini INFECTED AND UN-INFECTED INDIVIDUALS IN KHON KAEN PROVINCE May Soe Thwe, 1,2* Banchob Sripa, 1,2 Pattapong Kessomboon, 3 Sutas Suttiprapa 1,2 1 Tropical Disease Research Center, Department of Pathology, Faculty of Medicine, Khon Kaen University, Muang Khon Kaen, 40002, Thailand 2 Tropical Medicine Graduate Program, Faculty of Medicine, Khon Kaen University, Muang Khon Kaen, 40002, Thailand 3 Department of Community Medicine, Faculty of Medicine, Khon Kaen University, Muang Khon Kaen, 40002, Thailand * maythwe89@gmail.com Abstract: Melioidosis caused by Burkholderia pseudomallei is an important disease that is endemic in Southeast Asian countries including the Northeast Thailand and Northern Australia. In addition, a carcinogenic liver fluke, Opisthorchis viverrini (Ov), infection is endemic in the Greater Mekong Subregion, particularly in the Northeast Thailand where it is overlapping with melioidosis. One microbiome study of worms and bile of Ov infected hamsters showed that Ov contains several bacteria including Leptospira and Burkholderia genus. One recent study proved that Ov is a reservoir of Leptospira and anti-leptospira antibody is higher in Ov infected individuals than in Ov non-infected individuals. Here, we investigate whether Ov can effect on antibody production of B. pseudomallei in human serum samples by indirect hemagglutination (IHA) test. For this purpose, a case-control study was undertaken and 64 serum samples of 32 Ov infected and 32 Ov non-infected individuals aged 29 to 60 years were recruited from the Khon Kaen province in the Northeast Thailand. Of 64 sera, we found 7 seropositives in Ov infected individuals and 11 seropositives in Ov non-infected individuals. It seemed to be higher in Ov-uninfected individuals but it was not significantly difference (p=0.27). Other variants such as age and gender didn t show any significant difference with IHA titers. In conclusion, overall serologically diagnosed (IHA 1:160) melioidosis cases in Khon Kaen was 28% (18/64 cases) and Ov infections may have correlation with the anti-b. pseudomallei antibody. However, owing to low sample size in this study, the association between opisthorchiasis and melioidosis warrant further study. 162 Science and Technology in the Disruptive Era

169 B2_010_PA: MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM β-lactamase- PRODUCING Escherichia coli IN COMPANION ANIMALS May Thet Paing Phoo, 1 Rattanaruji Pomwised, 1 *Ruttayaporn Ngasaman 2, Arnon Chukamnerd 3 Krittika Dummunee 4 1 Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand 2 Department of Veterinary Public Health, Faculty of Veterinary Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand 3 Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand 4 Department of Microbiology, Faculty of Medicine, Princess of Naradhiwas University, Narathiwat, Thailand * rattanaruji.p@psu.ac.th Abstract: Background: Emergence of acquired extended spectrum β-lactamase (ESBL)-producing bacteria in companion animals is a global public health worrisome due to limitations in therapeutic options. Especially, ESBL-producing Enterobacteriaceae occurring in companion animals is a possible reservoir for antibiotic resistance in humans because of close-contact between them. The aims of this study were to investigate β-lactams susceptibility and to characterize ESBL genes in E. coli isolated from companion animals. Methods: A total of 55 phenotypically identified E. coli isolates were consecutively collected from dogs, cats and others (muntjacs, bovines, pigs and giraffes) admitted in Veterinary Teaching Hospital, Prince of Songkla University over a one-and-half-year period. Thirty-seven E. coli were confirmed as E. coli by PCR and then subjected to β-lactams susceptibility using disk diffusion test. ESBLs production was confirmed by combined disk test. ESBL-producing strains from phenotypic combined disk test were subjected to multiplex PCR to detect ESBL genes (bla TEM, bla SHV and bla CTX-M). Results: In this study, 5 of 23 E. coli isolates from dogs (22%) and 3 of 8 from other animals (38%) were confirmed phenotypically and genotypically as ESBLs producers. Surprisingly, none of isolates from cats are ESBL producers. Two isolates (5.4%) were found to harbor bla TEM genes, 4 isolates (10.8%) carried bla CTX-M genes and 2 isolates (5.4%) harbored both bla TEM and bla CTX-M genes. Conclusions: Incidence of ESBL in dogs and other animals found to be high while that of cats still remained low. It might be because of small size of our samples. Further nationwide study should be done with large amount of samples to know the distribution of ESBL in companion animals. However, our data demonstrated that E. coli isolated from companion animals produced ESBLs and harbored ESBL genes. Therefore, they can also be a reservoir for antibiotic resistances. 163 The 44 th Congress on Science and Technology of Thailand (STT 44)

170 B2_011_OF: CHARACTERIZATIONS OF CIPROFLOXACIN RESISTANCE IN Klebsiella pneumoniae ISOLATED FROM PATIENTS IN MEDICINE WARD, SONGKLANAGARIND HOSPITAL Arnon Chukamnerd, 1 Sarunyou Chusri, 2 Rattanaruji Pomwised, 3* May Thet Paing Phoo 3 1 Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand 2 Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand 3 Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand * rattanaruji.p@psu.ac.th Abstract: The objective was to investigate plasmid-mediated quinolone resistance (PMQR) genes in K. pneumoniae isolated from patients in medicine ward, Songklanagarind hospital. Two hundred and twenty-three bacterial isolates were recovered from rectal and throat swabs of patients admitted to the medicine ward, Songklanagarind hospital. Thirty-five of 223 isolates (15.7%) were identified as Klebsiella pneumoniae by biochemical testing. The minimum inhibitory concentrations (MICs) of ciprofloxacin for all K. pneumoniae isolates were determined by broth microdilution method. Twenty-two of 35 K. pneumoniae isolates (62.9%) were resistant to ciprofloxacin with MIC ranged 4 to 256 µg/ml. The PMQR determinants including qnra, qnrb, qnrs, qepa, and aac(6 )-Ib-cr genes were determined in all K. pneumoniae isolates by PCR method. The aac(6 )-Ib-cr gene was mostly found (16 isolates), followed by qnrb gene (4 isolates), and qnra gene (2 isolates). None of qnrs and qepa genes was present in any isolates. Among 35 K. pneumoniae isolates, 2 isolates (5.7%) harbored 2 genes (qnrb and aac(6 )-Ib-cr) and 3 isolates harbored 3 genes (qnra, qnrb, and aac(6 )-Ib-cr). 164 Science and Technology in the Disruptive Era

171 B2_012_PF: SCREENING, PRODUCTION AND CHARACTERIZATION OF A HYDROPHOBIN PROTEIN FROM WHITE ROT FUNGI Nianrawan Meecharoen, 1 Chetsada Pothiratana, 1 Surachai Thachepan 2, Lerluck Chitradon 1 Churapa Teerapatsakul 1, * 1 Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand 2 Kamnoetvidya Science Academy, Rayong 21210, Thailand * fscicpt@ku.ac.th Abstract: Hydrophobins are important surface active amphipathic proteins, produced exclusively by filamentous fungi. They have the ability to modify surface properties by interfacial self-assembly with potential for several applications including enhancing drug bioavailability, immobilizing enzymes, as food stabilizers, and as low friction coatings on biomaterials. White rot fungi from Thailand were screened to ascertain the relationship between colony surface hydrophobicity and amount of extracted hydrophobins. Water contact angle (WCA) value was determined as a primary screening test to indicate colony surface hydrophobicity. Among fifteen white rot fungal strains tested, a newly isolated fungus Fomes fomentarius BKNO1 showed the highest WCA value for colony surface. Strain BKN01 also gave the highest yield of hydrophobin protein at 5.3 mg g -1 of mycelial cell dried weight. The expected 9.1 kda protein band was observed in the hydrophobin extract of strain BKN01 and amino acid sequences of the hydrophobin protein were also analyzed. When the extracted hydrophobin from strain BKN01 was coated on a glass slide, the WCA value significantly increased with wettability changing from hydrophilic to almost hydrophobic. 165 The 44 th Congress on Science and Technology of Thailand (STT 44)

172 B2_013_PA: FUNCTIONAL CHARACTERIZATION OF RESPONSIVE EFFLUX TRANSPORTATION OF ANTIBACTERIAL COMPOUNDS IN Pseudomonas aeruginosa Tunyalux Huangsuwannakorn 1, Nisanart Charoenlap 2, Paiboon Vattanaviboon 1, 2, Skorn Mongkolsuk 1, 2, 3, * 1 Applied Biological Sciences Environmental Health Program, Chulabhorn Royal Academy, Chulabhorn Graduate Institute, Bangkok, Thailand 2 Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, Thailand 3 Department of Biotechnology, Faculty of Science, Mahidol University Bangkok, Thailand * Skorn@cri.or.th Abstract: Pseudomonas aeruginosa is a Gram-negative bacterium that is classified as a critical opportunistic pathogen. P. aeruginosa has been in the spotlight of global public health concern because of the prevalence of morbidity and mortality in the past decades via serious infections in immunocompromised persons and individuals with cystic fibrosis and severe burns. Due to its natural resistance to wide range of antibiotics through varieties of mechanisms and pathways, P. aeruginosa infections are difficult to be treated. The multidrug efflux system plays a vital role in transporting toxic substances out of bacterial cells, conferring to their resistance phenotypes. This study will focus on the characterization of efflux pumps to understand the mechanisms of antibiotic resistance and serve as a potential target to treat P. aeruginosa infection. A gene cluster of four genes, marr, omp, mrp and det, are localized in proximity to each other according to sequence alignment encoding for transcriptional regulator, outer membrane protein precursor, multidrug resistance protein and drug efflux transporter, respectively, in P. aeruginosa. The mutant strains lacking these four genes were constructed by gene inactivation technique using a suicide plasmid vector pknock-gm. Phenotypic analysis by antibiotic susceptibility revealed that all the knockout mutants showed no significant difference in antibiotic resistance levels compared to PAO1 wild-type strain. However, overexpression of mrp and det genes in the PAO1 showed more resistant to fluoroquinolone antibiotics compared to wild-type strain. The result suggested that mrp and det are responsible for efflux transport of quinolone drugs. In conclusion, mrp and det genes contribute to the transportation of fluoroquinolone antibiotics and may be one of the possible systems accounted for multiple drug resistance of P. aeruginosa. Thus, mrp and det might serve as efficient targets for drug development in the future. 166 Science and Technology in the Disruptive Era

173 B2_014_PF: CHARACTERIZATION OF EXTENDED SPECTRUM BETA-LACTAMASE-PRODUCING Escherichia coli ISOLATED FROM PATIENTS IN MEDICINE WARD, SONGKLANAGARIND HOSPITAL Chanitnart Phaothong, 1 Rattanaruji Pomwised, 1* Sarunyou Chusri 2 1 Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand 2 Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai Campus, Songkhla, Thailand * rattanaruji.p@psu.ac.th Abstract: Two hundred and twenty-three bacterial isolates were obtained from rectal and throat swabs of patients who were admitted to the medicine ward, Songklanagarind hospital. All of bacteria were identified based on biochemical method and molecular method using uida gene as a marker. Thirty-eight of 223 isolates (17.04%) were identified as Escherichia coli and then were tested for antibiotic susceptibility to twelve antibiotics. Most isolates resisted to ampicillin (78.9%), ciprofloxacin (50.0%), cefotaxime (34.2%) and ceftazidime (31.6%). Most of them were still susceptible to cefoxitin (71%) and imipenem (68.4%). Phenotypic confirmation test for extended spectrum beta-lactamase (ESBL) production was performed in all E. coli isolates by combination disk diffusion. Out of 38 isolates, 11 isolates produced ESBLs. The presence of bla TEM, bla SHV and bla CTX-M in all ESBL-producing isolates were investigated by multiplex PCR method. The bla TEM was the most harbored in these E. coli (11 isolates), followed by bla CTX-M (5 isolates). None of bla SHV was discovered in any isolates. Among 11 ESBLs producers, 10 isolates (90.9%) harbored only one ESBL gene subtype. Four isolates (36.4%) harbored two different ESBL gene subtypes including bla TEM and bla CTX-M. Other mechanisms of beta-lactam resistance including present of bla AmpC and carbapenemases gene and efflux pump modification will be further investigated. 167 The 44 th Congress on Science and Technology of Thailand (STT 44)

174 B2_015_PA: FUNCTIONAL CHARACTERIZATION OF RBN1 AND RBN2 IN Stenotrophomonas maltophilia Veerakit Vanitshavit 1, Nisanart Charoenlap 2, Skorn Mongkolsuk 2,3, Paiboon Vattanaviboon 1,2 * 1 Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand 2 Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, Thailand Department of Biotechnology, Faculty of Science, Mahidol University Bangkok, Thailand * paiboon@cri.or.th Abstract: Stenotrophomonas maltophilia is a gram-negative bacterium commonly found in the environments such as soil, water and plants. This bacterium cause hospital-acquired infections especially in immunocompromised patients. S. maltophila has several factors contributing to its pathogenesis including biofilm formation, antioxidant enzyme production and antibiotic resistance. Bacterial RNases have different functions in cellular activities and processes including RNA stability and degradation, bacterial pathogenicity, antibiotic resistance and virulence factors. In this study, putative ribonuclease genes of S. maltophilia K279a including rbn1 and rbn2 were functionally characterized. The expression of rbn1 was regulated by SoxR, a superoxide sensing regulator and could be induced by superoxide generating agents such as pumblagin, paraquat and menadione. The rbn2 was located next to rbnr, which encodes regulatory protein belonging to a LysR family, in a head-to-head fashion. The expression of both rbnr and rbn2 were induced by ribosome targeting antibiotics i.e. tetracyclines, kanamycin, streptomycin and neomycin, suggesting that rbnr and rbn2 share the same inducible transcriptional regulator. As rbnr is a regulatory protein, the possibility that RbnR regulates rbn2 expression is being investigated. The antibiotic susceptibility phenotype was investigated in both rbn1 and rbn2 mutants. The results showed that only rbn2 mutant was more susceptible to aminoglycoside drugs than its parental wild-type. This phenotype together with the rbn2 gene expression pattern that is inducible by ribosome targeting antibiotics suggest an importance role of rbn2 on antibiotic resistance of S. maltophilia. The precise mechanism of Rbn2 RNases on resistance against ribosome targeting antibiotics need further studies. The knowledge of Rbn2 importance on antibiotic resistance could be applied for a novel antimicrobial resistance bacteria treatment strategy; blocking the function of Rbn2 would render the resistant bacteria become susceptible to commonly used antibiotics. 168 Science and Technology in the Disruptive Era

175 B2_016_OF: ISOLATION OF BIOSURFACTANT PRODUCING BACTERIA FROM THAI TRADITIONAL FERMENTED SOYBEAN FOOD Supitchaya Laothong 1, Vongsathorn Ngampuak 1, Chakrit Tachaapaikoon 1,2, Patthra Pason 1,2, Natta Laohakunjit 1, Khanok Ratanakhanokchai 1 and Rattiya Waeonukul 1,2 * 1 School of Bioresources and Technology, King Mongkut s University of Technology Thonburi (KMUTT), 49 Soi TienThale 25, Bangkuntien-Chaithale Road, Bangkuntien, Bangkok Pilot Plant Development and Training Institute (PDTI), King Mongkut s University of Technology Thonburi (KMUTT), 49 Soi TienThale 25, Bangkuntien-Chaithale Road, Bangkuntien, Bangkok * rattiya.wae@kmutt.ac.th Abstract: Biosurfactants are amphiphilic compounds produced by microorganisms and have various uses in industry. They can replace the chemical surfactants because of biodegradability, non-toxicity and their ability to be produced from renewable sources. However, a major obstacle in producing the biosurfactant at the commercial scale is the lack of cost effectiveness. In this study, the selection of the effective strain and the use of low-cost agro-industrial residues, soybean meal as substrate for producing biosurfactant, not only is the production cost reduced but a higher production yield is also achieved. The results showed that one hundred twenty six morphologically distinct colonies were isolated from Thai traditional soybean foods. For screening of the most potent biosurfactant strains, the isolated bacteria were selected by using four methods as oil displacement, drop collapse, emulsification and hemolytic activity test. Among them, only twelve isolates showed positive results for all the screening methods. The strain B15-1, the most effective biosurfactant producer showed the highest biosurfactant production of 251 mg/l in soybean meal medium and maximum reduction in surface tension at 30.44±0.02 mn/m. The 16S rrna analysis revealed that the isolated strain B15-1 showed 99% similarity to Bacillus subtilis. Preliminary chemical characterization revealed that the biosurfactant from the isolated strain B15-1 has lipopeptide composition confirmed by Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis. Therefore, these results suggested that the production of biosurfactant by the effective strain B15-1 and using agro-industrial residues, soybean meal as substrate is one of the promising ways for improving the economics of biosurfactant process. 169 The 44 th Congress on Science and Technology of Thailand (STT 44)

176 B2_017_PA: APPLICATION OF BIO-BASED WASHING FORMULATION AND MIXED BACTERIA FOR REMEDIATION OF PETROLEUM-CONTAMINATED SOIL Rattiya Padungpol, 1 Boonyisa Suksomboon, 2 Noulkamol Arpornpong, 3 Ekawan Luepromchai 1,2, * 1 Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management, Chulalongkorn University, Bangkok, Thailand 3 Department of Natural Resource and Environment, Faculty of Agriculture Natural Resource and Environment, Naresuan University, Phitsanulok, Thailand * ekawan.l@chula.ac.th Abstract: Petroleum drilling activity generates large amount of cutting waste, which is composed of soil and rock particles contaminated with synthetic-based drilling mud (mostly containing aliphatic hydrocarbons). The objectives of this study were to formulate a bio-based washing solution for reducing hydrocarbon concentrations in the contaminated cutting and to use mixed bacteria for degrading the remaining hydrocarbon. The suitable washing formulation contained 20% biosurfactant and 4% Dehydol LS7TH, which showed the highest total petroleum hydrocarbon (TPH) removal efficiency at 93%. The phytotoxicity test found that the initial hydrocarbon-contaminated soil was toxic for green bean seeds as shown by the germination efficiency at 0%. After soil washing, the green bean seeds were germinated at 100%. However, TPH still remained in the soil at 4,000-10,000 mg/kg. Thus, the bioremediation by mixed bacteria combined with landfarming was conducted under laboratory scale. The mixed hydrocarbon-degrading bacteria included Marinobacter salsuginis RK5, Microbacterium saccharophilum RK15, and Gordonia amicalis JC11. After 49 days of remediation, the treatment with biochar, fertilizer, and mixed bacteria had the highest TPH removal efficiency at 71%. In the future, the efficiency of this sequential soil washing and landfarming should be confirmed in a large-scale experiment for contaminated soil remediation. 170 Science and Technology in the Disruptive Era

177 B2_018_PF: THE INFLUENCE OF WATERING SOIL AND THALLUS ON PHYSIOLOGICAL PROCESSES AND NATURAL PRODUCTS OF THE TRANSPLANTED LICHEN Parmotrema tinctorum Mongkol Phaengphech, 1 * Prichukorn Khongsatra, 2 Wetchasart Polyiam, 1 Chutima Sriviboon, 2 Kansri Boonpargob 1 1 Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand 2 Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand * mongkolpp@gmail.com Abstract: Lichen produces secondary metabolites that are different from other organisms and have great potential to be utilized commercially. However, lichens have extremely low growth rate due to their photosynthesis active only during high atmospheric humidity, which only exist in early morning. The objective of this study was to transplant the lichen Parmotrema tinctorum on man-made substrate, and provide extra water through soil watering, and direct spraying on thallus. It was hypothesized that additional water could enhance lichen production. The transplantation was conducted at Khao Yai National Park by using 200 fragmented thalli. They composed of four treatments: 1) without soil watering-without thallus watering (SD-TD), 2) without soil watering thallus watering (SD-TW), 3) soil watering without thallus watering (SW-TD), 4) soil watering thallus watering (SW-TW). After a year of transplantation, we found that the lichens received extra water through soil watering and thallus spraying (SW-TW) had the highest growth rate measured 9.85 mm y -1, while the highest biomass production of 56.4 g g -1 was measured from soil watering alone (SW-TD). Contrary, photosynthesis (P N) and Fv/Fm of both treatments declined. More importantly, daily soil watering alone (SW-TD) without thallus spraying, increased growth, biomass and P N of the lichens more than only thallus spraying (SD-TW), once in two weeks. This treatment should be taken into consideration for future transplantation as well. Lecanoric acid was the main natural product that P. tinctorum metabolized. This substance and methyl orsellinate, the minor substance, declined in all treatments after transplantation. Contradictory, orsellinic acid increased in all treatments. Whereas, atranorin, the second main substance, had varying amounts. It is most essential to continue research and development on lichen transplantation to achieve the maximum production of lichens by using the information from this study as a basis. 171 The 44 th Congress on Science and Technology of Thailand (STT 44)

178 B2_019_PA: IDENTIFICATION AND CHARACTERIZATION OF LIPASE FROM Virgibacillus halodenitrificans SKP5-4 Supalurk Yiamsombut, 1* Rungsima Daroonpunt, 2 Ancharida Savarajara, 3 Wonnop Vissessanguan 4 and Somboon Tanasupawat 2 1 Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3 Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 4 National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani 12120, Thailand * Supalurk.Y@chula.ac.th Abstract: Lipases are commercially significant in food industry in the production of a variety of products, ranging from fruit juices, baked foods, vegetable fermentation and in lavor development. In our screening of bacterial lipase, a moderately halophilic bacterium, strain SKP5-4, was isolated from shrimp paste (Ka-pi) sample collected from Samut Sakhon province, Thailand. The strain was identified as Virgibacillus halodenitrificans based on the morphological, physiological and biochemical characteristics including 16S rrna gene sequence analysis (99.5% similarity). This strain showed high lipolytic activity ( Unit/mL) and was selected for lipase optimization. The enzyme of strain was purified 12-fold with 4% final yield to homogeneity by 40-60% cold acetone precipitation, Hitrap DEAE FF anion exchange chromatography. The molecular mass of the lipase activity was estimated to be 50, 43, 30 and 12 kda by native-page. The optimum conditions for purified lipase was at ph 7.0, 40ºC temperature, 2% NaCl and specific substrate activity revealed that maximum activity only with p-nitrophenyl butyrate (p-npb). These features of the enzyme may have benefit potential for industrial applications. 172 Science and Technology in the Disruptive Era

179 B2_020_PA: A NOVEL Actinoplanes ISOLATED FROM LICHEN AND ITS ANTIMICROBIAL ACTIVITY Patcharin Saeng-in, 1* Wongsakorn Phongsopitanun, 2 Ancharida Savarajara, 1 and Somboon Tanasupawat 3 1 Department of Microbiology, Faculty of Sciences, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand 3 Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkoarn University, Bangkok 10330, Thailand * patcharin_saeng-in@hotmail.com Abstract : A novel species of the genus Actinoplanes, strain LDG1-06 was isolated from a lichen sample collected from tree bark, Mahasarakham province in the north-eastern part of Thailand. The taxonomic position of the species has been described based on a polyphasic approach. Strain LDG1-06 produced irregular sporangia on agar media. It contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell wall peptidoglycan was found to be glycolyl. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. Whole-cell hydrolysates contained galactose, glucose, mannose and small amounts of xylose. Major menaquinones were MK-9(H 4) and MK-9(H 6), and major cellular fatty acids were iso-c 16:0 (34.4 %), anteiso-c 15:0 (20.0 %) and iso-c 15:0 (16.3 %). 16S rrna gene sequence analysis of strain LDG1-06 showed high similarity to Actinoplanes deccanensis IFO T (98.4 %) and it clustered with Actinoplanes brasiliensis DSM T (98.2 %) Actinoplanes bogorensis LIPI11-2-Ac043 T (98.2 %) and Actinoplanes abujensis A4029 T (97.9 %) in phylogenetic tree analysis. This isolate exhibited antimicrobial activity against Candida albicans ATCC The 44 th Congress on Science and Technology of Thailand (STT 44)

180 B2_021_PA: BIODEGRADAION OF CRUDE OIL BY INDIGENOUS BACTERIAL CONSORTIUM ISOLATED FROM GULF OF THAILAND S SEDIMENT UNDER VARIOUS ENVIRONMENTAL CONDITIONS Chanokporn Muangchinda, 1 Piyanoot Uklam, 1 Penjai Sompongchaiyakul, 2 Onruthai Pinyakong 1,3 * 1 Microbial Technology for Marine Pollution Treatment Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 3 Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management (HSM), Bangkok, Thailand * onruthai.p@chula.ac.th Abstract: Crude oil contamination is a major concern for environment and health. Bioremediation by indigenous microorganisms has been suggested for removing crude oil from contaminated sites because they were likely adapted to the environment requiring treatment. The purpose of this study was to investigate the potential of indigenous crude oil-degrading bacterial consortium for biodegradation of crude oil under various conditions. The bacterial consortium (G11) was isolated from Gulf of Thailand s sediment using crude oil as the sole carbon source. It could degrade 80% of 0.5% (v/v) crude oil within 5 days. The consortium G11 was able to degrade maximum crude oil at the rate of 6.75 mg/l/g cells/day and degrade crude oil in a wide range of salinities from 20 to 200 ppt, ph values from 3 to 9 and temperatures from 30 to 37 C. A high degradation was found in all tested conditions. More than 70% of 0.5% (v/v) crude oil was degraded after 12 days of incubation in all treatments. Moreover the consortium G11 degraded a wide range of petroleum compounds including diesel oil, fuel oil, gasohol 91, gasohol 95, synthetic engine oil, semi synthetic engine oil, tetradecane, hexadecane and a mixture of polycyclic aromatic hydrocarbons (PAHs) consisting of anthracene, phenanthrene, fluoranthene, and pyrene. In addition, the culture-independent and culture-dependent methods were used to analyse bacterial structures that are present in the consortium. Four bacteria including Pseudomonas sp., Achromobacter sp., Stenotrophomonas sp. and Staphylococcus sp. were isolated from the consortium, while the 16S rrna gene amplicon sequencing analysis demonstrated that Methylophaga Pseudomonas, Achromobacter, Alcanivorax and Stenotrophomonas were present in the consortium G11. Our results suggest that the consortium G11 will be useful for remediation of crude oil-contaminated site in a wide range of environmental conditions. 174 Science and Technology in the Disruptive Era

181 SESSION B: BIOLOGICAL SCIENCE (B3: MOLECULAR BIOLOGY) 175 The 44 th Congress on Science and Technology of Thailand (STT 44)

182 B3_001_PF: PATTERNS OF SPECIFIC IGE BOUND WHEAT PROTEINS OF INDIVIDUALS SUFFERED WHEAT INDUCED ANAPHYLAXIS Pisit Ubonsri 1, Surapon Piboonpocanun 1* 1 Institute of Molecular Biosciences, Mahidol University, Nakhonpathom 73170, Thailand *Corresponding author s piboons@gmail.com Abstract: Wheat storage proteins, one of 8 major food allergens, cause mild to severe allergic reactions in multi-organs including anaphylaxis through cross-linking with serum specific IgE in sensitized individuals. Wheat induced anaphylaxis (WA) in wheat sensitized Thai individuals have been observed. Early diagnosis to identify a pattern of specific IgE bound wheat proteins may provide a helpful information to individuals who sensitize to wheat protein to avoid contacting or consuming products containing wheat proteins. This project aimed to determine pattern of specific IgE bound wheat proteins from individuals who suffer wheat induced anaphylaxis. Wheat allergens were prepared from wheat flour based on their biochemical properties. Immunoblot using serum specific IgE from 10 WA Thai individuals was performed. Wheat salt soluble (WSS) proteins were extracted from wheat flour before wheat gliadins (WG) was extracted from the pellet with buffer containing alcohol. Patterns of IgE binding WSS showed 4 specific IgE bound to proteins at molecular weight (MW) of ~30, ~37, ~45, and~50 kda, as major allergens. Patterns of IgE bound to WG showed 2 serum specific IgE bound to WG proteins at MW of ~37 and ~45 kda, as major allergens. Four WSS and two WG proteins appear to be major allergens to which > 80% of serum IgE of WA Thai donors bind. 176 Science and Technology in the Disruptive Era

183 B3_002_PF: MOLECULAR CLONING AND FUNCTIONAL STUDY OF Penaeus monodon RAB11-FIP2 Suphattha Kaeomani, 1 Sakol Panyim, 1,2 Chalermporn Ongvarrasopone 1, * 1 Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand 2 Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand * chalermporn.ong@mahidol.ac.th Abstract: Rab11 is a member of Ras-related small GTPase superfamily which plays a role in transporting vesicles to plasma membrane. Previous study found that Rab11 is important for vesicle transport of Penaeus monodon. The active form of Rab11 requires effector proteins, called Rab11-family interacting proteins (Rab11-FIPs). Rab11-FIP2 is a protein that links a motor protein with Rab11 and allows vesicle to slide along actin filament. Therefore, this work aims to clone and investigate the function of P. monodon Rab11-FIP2 (PmRab11-FIP2) using RNA interference technology. The partial 1.4-kb cdna of PmRab11-FIP2, including 5ˊ UTR of size 281 bp, was obtained and could be translated to 445 amino acids of the partial PmRab11-FIP2 protein. Sequences analysis demonstrated that the partial PmRab11-FIP2 protein contained the conserved domains, consisting of C2 and Rab11-binding domains. The phylogenetic tree analysis showed that the partial PmRab11-FIP2 is closely related to other arthropods Rab11-FIP2. To study the function of PmRab11-FIP2 using RNAi technology, the dsrna-pmrab11-fip2 was produced using in vivo bacterial expression systems and then injected into 3-5 g shrimps. The dsrna-pmrab11-fip2 at 1.25 μg/g shrimp could inhibit PmRab11-FIP2 expression at 24 hours post injection. These results suggested that the dsrna-pmrab11-fip2 could suppress the expression of PmRab11-FIP2. Therefore, it can be used to study the effect of PmRab11-FIP2 knockdown during viral infection in shrimp. Keywords: RNA interference/ Penaeus monodon/ vesicle transport/ Rab11-FIP2 177 The 44 th Congress on Science and Technology of Thailand (STT 44)

184 B3_003_PF: CHARACTERIZATION OF CRUSTIN RESPONDED TO Vibrio parahaemolyticus AHPND INFECTION IN Litopenaeus vannamei Chadapa Sakunwattana 1, Pichit Olanwongsakul 1, Patamaporn Umnahanant 1, Sirinit Tharntada 1 * 1 Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand * sirinit.t@ku.ac.th Abstract: Acute hepatopancreatic necrosis disease (AHPND) is the important virulent disease. It causes mortality in shrimp. Crustins are famous antimicrobial peptides (AMPs) in Litopeneuas vannamei. These proteins are in antibacterial and in anti-proteinase activities. This work is to study differential expression of CrustinLv4, Crustin-likeLv and SWD77 after infected with Vibio parahaemolyticus AHPND. The results in time-courses analysis for the gene expression profile show CrustinLv4 was up-regulated at 24 h and 48 h, Crustin-likeLv was up-regulated at 48 h and SWD77 was up-regulated at 48 h after VP AHPND infection. The recombinant CrusLv4 protein was expressed in Escherichia coli system. The results of rcruslv4 expression showed that rcruslv4 (~15 kda) was expressed in E.coli BL21CodonPlus (DE)-RIL at 3-6 h after induction with IPTG. The protein was not expressed in E.coli BL21 (DE3) PlysS and E.coli Roseta DE3 compare to the non-induction with IPTG. CrustinLv is a part of an important immunity gene and may serve an important function in defense against bacteria. Key words: Acute hepatopancreatic necrosis disease (AHPND), Crustin, Litopeneuas vannamei 178 Science and Technology in the Disruptive Era

185 B3_004_PF: CLONING AND EXPRESSION OF A HUMAN HISTONE DEACETYLASE 1 GENE IN Escherichia coli Nutthachai Lertamornthum 1, Gulsiri Senawong 1,Duanggamon Muengsaen 1, Kanoknan Khongsukwiwat 1, Thanaset Senawong 1,2, * 1 Department of Biochemistry, Faculty of Science, Khon Kaen University, Thailand 2 Natural Product Research Unit, Faculty of Science, Khon Kaen University, Thailand * sthanaset@kku.ac.th Abstract: Histone deacetylases (HDACs) regulate gene expression in eukaryote by promoting the removal of acetyl group from lysine residue on histone protein. HDACs are known to play a pivotal role in the regulation of cell proliferation. Therefore, inhibition of HDACs has become an interesting approach for anti-cancer therapy. The aims of this study were to clone and express the human HDAC1 gene in E. coli. Total RNA was extracted from HeLa cells and used as a template for the synthesis of cdna. A full-length of human HDAC1 cdna was amplified by PCR. The hhdac1 cdna was cloned into pet-15b at BamHI site and expressed in E. coli BL21(DE3). The expressed proteins were found in an insoluble form. Consequently, the hhdac1 cdna was sub-cloned into BamHI and XhoI site of pgex-6p-2 containing Glutathione S-transferase (GST) at N-terminal. The pgex-6p-2 harboring hhdac1 cdna was transformed into E. coli BL21(DE3) plyss. The gene was overexpressed by induction with IPTG. However, the expressed recombinant proteins were still found in an inclusion body. Accordingly, alternative host cell types for expression of the recombinant hhdac1 may be required to obtain a soluble protein which will be used for screening of HDAC inhibitors from natural products and synthetic compound library. 179 The 44 th Congress on Science and Technology of Thailand (STT 44)

186 B3_005_OF: CONVOLUTIONAL NEURAL NETWORK AS A TOOL FOR HIGH-THROUGHPUT IDENTIFICATION OF REGULATORY SEQUENCES IN GENOMIC DNA Siwat Ruangroengkulrith, 1 Nicholas Keone Lee, 2,3 Martin Hemberg, 2, * Varodom Charoensawan 1,4,5 * 1 Department of Biochemistry, Faculty of Science, Mahidol University, 272 Rama 6 Rd, Bangkok 10400, Thailand. 2 Wellcome Sanger Institute, Hinxton, Cambridge, UK 3 University of Cambridge, Cambridge, UK 4 Integrative Computational BioScience (ICBS) Center, Mahidol University, Nakhon Pathom, Thailand. 5 Systems Biology of Diseases Research Unit, Faculty of Science, Mahidol University, Bangkok, Thailand. * varodom.cha@mahidol.ac.th (V. Charoensawan), mh26@sanger.ac.uk (M. Hemberg) Abstract: The complex regulation of gene transcription is a result of the physical interaction between the transcription factor (TF) proteins and promoter or enhancer sequences on DNA. In metazoans, TFs bind to short (6-12 bps) DNA sequences, also referred to as motifs, and knowledge of where a TF binds provides important information for understanding gene regulation in development and disease. Recently, the massively parallel reporter assays (MPRAs) provide an opportunity for high-throughput identification of functional DNA features. However, a suitable method for extracting such information is by no mean widely established and involved many challenges. Here, we present the application of a convolutional neural network (CNN) for de novo discovery of binding motifs and their impact on the transcriptional activity in two MPRA datasets. The performance of CNN in predicting the enhancer activity was comparable to those conventional machine learning algorithms that require prior knowledge of TF binding sequences. Moreover, it can also accurately identify the known motifs that are present within the enhancer sequences. Taken together, we have demonstrated the prospect of using CNN for DNA motifs analysis and discovery of known and new regulatory sequences, which can also be applied to other large-scale reporter assays in the future 180 Science and Technology in the Disruptive Era

187 B3_006_PA: THE GENERATION OF STABLE, TRUNCATED FORMS OF Macrobrachium rosenbergii NODAVIRUS VIRUS-LIKE PARTICLES (VLPS) AND THE PRODUCTION OF PDGFR-TARGETING VLPS Atthaboon Watthammawut 1*, Monsicha Somrit 2* 1 Department of Anatomy, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand 2 Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand * atthaboon@g.swu.ac.th, monsicha.som@mahidol.ac.th Abstract: Cell-targeting and drug delivery using nanoparticles of synthetic biopolymers have the difficulty in controlling their local and on-site concentrations. This limited ability reduces the "retention effect" which is crucial for effective therapeutics. Our research team has instead focused on the production of biological protein containers/biologically-active icosahedral particles in the form of Macrobrachium rosenbergii Nodavirus virus-like particles (MrNV-VLPs). In the present study, we created MrNV-VLPs which can be further modified to present larger ligands/antigens while maintaining structural stability. We demonstrated the integrity of VLPs that had either a complete or lower degree of truncation of the P-domain (V251- and G264-VLPs) that were able to form icosahedral particles. V251-VLPs formed 20nm particles with molecular mass around 27 kda, while G264-VLPs formed 20-22nm particles with the MW of 30 kda. Both constructs exhibited particles sizes and MW lower than that of MrNV-VLPs (27-30nm/41.8 kda) Furthermore, we examined the possibility of creating biologically-active MrNV-VLPs that could potentially target and affect PDGFRα-positive cells such as fibroblasts or mesenchymal-derived cells which cause fibrotic pathology. We were able to generate fully icosahedral-forming MrNV-VLPs presenting an extension to the C-terminus of the P-domain consisting of a short mimetic PDGF domain (Mimetic BiPDGF peptide-mrnv-vlps). Moreover, we were able to show the VLPs PDGFRα-targeting capabilities in low-moderate PDGFRα-receptor expressing SH-SY5Y neuroblastoma cells. These findings pave the way for future binding experiments of mimetic BiPDGF peptide-mrnv-vlps in a more reduced or no P-domain form in high PDGFRα expressing cells and tissues. Objective: To generate and examine the icosahedral-forming capabilities of MrNV-VLPs that have their P-domain fully truncated (V251-MrNV-VLPs) or truncated to a predicted calcium-binding domain (G264-MrNV-VLPs); and the generation of PDGFRα- receptor VLPs (mimetic BiPDGF peptide-mrnv-vlps) and their binding patterns in PDGFRα- receptor positive SH-SY5Y neuroblastoma cells. Materials and Methods: P-domain-truncated V251-MrNV-VLPs and G264-MrNV-VLPs; mimetic BiPDGF peptide-mrnv-vlps and scrambled mimetic peptide-mrnv-vlps were generated by MrNV capsid gene editing and cloning. Gene constructs were expressed in E. coli and recombinant proteins purified by affinity FPLC. Virus particles were visualized negative staining and observed under TEM. VLPs targeting PDGFR receptors were incubated with PDGFR+ve SH-SY5Y cells and observed using FL-microscopy. Results: Figure. 1. Native MrNV-VLPs. MrNV-VLPs were expressed from RNA2 derived capsid gene comprising 371 amino acids (A to D). E shows the negative staining of MrNV-VLPs, showing T=3 icosahedral particles with sizes of nm. F shows a single particle 3D reconstruction from negatively stained particles to a resolution of 16 angstroms. 181 The 44 th Congress on Science and Technology of Thailand (STT 44)

188 B3_007_PF: FUNCTIONAL CHARACTERIZATION OF A PATHOGEN RESPONSIVE microrna IN BLACK TIGER SHRIMP Penaeus monodon Nichaphat Kanoksinwuttipong and Kunlaya Somboonwiwat* Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd, Wangmai, Pathumwan, Bangkok 10330, Thailand. * ningnichaphat.k@gmail.com Abstract: RNA interference (RNAi) is a biological process in which RNA molecules such as microrna and sirna play a role in transcriptional regulation. MicroRNA (mirna) is small noncoding-rna that plays an important role in immune response by regulating the target gene expression at post-transcription level. In our previous study, mir-750 was a differentially expressed mirna identified in white spot syndrome virus (WSSV)-infected Penaeus monodon. Bioinformatic analysis suggested that the predicted target mrnas of mir-750 were UbcQ1 and VPS53. Furthermore, the mirna/mrna interaction was analyzed using RNA hybrid program. The stem-loop real-time RT-PCR technique demonstrated significant alteration of mirna expression upon WSSV infection indicating their possible involvement in shrimp antiviral response. In addition, the expression pattern of mirna target genes was analyzed. The correlation of expression pattern between mir-750 and its target genes inferred that mir-750 might target UbcQ1 which contain 1,137 bp of the ORF encoding 378 amino acid according to RACE technique. It suggested the possible regulatory role of mirna in shrimp immunity against WSSV infection. The dual luciferase reporter assay was performed to verify the interaction between mir-750 and UbcQ1. No significant change in luciferase activity was observed after co-transfected of mir-750 mimic and luciferase reporter plasmid containing UbcQ1 target sequence suggesting that mir-750 might not directly target to UbcQ1. However, this mirna might play important role in shrimp immunity against WSSV infection, the function and its mrna target of mir-750 will be further characterized. 182 Science and Technology in the Disruptive Era

189 B3_008_PA: CLONING, OVEREXPRESSION, AND PURIFICATION OF A GENE OF UNKNOWN FUNCTION OF PROPHAGE LOCI FROM Candidatus Liberibacter asiaticus, A CAUSAL AGENT OF HUANGLONGBING DISEASE IN CITRUS PLANTS Nonnanat Yooyuen, 1 Duangtip Sudhan, 1 Thamrongjet Puttamuk, 2 Supachai Vuttipongchaikij, 3,4 Pitak Chuawong 1, 1 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Special Research Unit for Advanced Magnetic Resonance (AMR), Kasetsart University, Bangkok 10900, Thailand 2 School of Agriculture and Cooperatives, Sukhothai Thammathirat Open University, Nonthaburi 11120, Thailand 3 Department of Genetics, Faculty of Science, Kasetsart University, 50 Ngarm Wong Wan Road, Chatuchak, Bangkok 10900, Thailand 4 Center of Advanced Studies for Tropical Natural Resources, Kasetsart University, Ngam Wong Wan Road, Chatuchak, Bangkok 10900, Thailand * Pitak.C@ku.ac.th * Abstract: Huanglongbing (HLB), also known as citrus greening, is a destructive disease targeting all species of Citrus worldwide. The disease is transmitted via psyllids (Diaphorina citri Kuwayama) and grafting. Characteristic symptoms comprise blotchy-mottling, vein corking, yellowing, chlorotic shoots, and die-back on the trees. Upon infection, the fruit quality is worsened, and the infected tree subsequently dies in 3 5 years. The causal agent of this disease in Asia is Candidatus Liberibacter asiaticus (CLas), a phloem-limited, Gram-negative bacterium in the alpha subdivision of proteobacteria. Within the genome of CLas lies prophage regions. The prophage/phage-mediated dynamics of CLas population were categorized as Type-A, B, C, and D. Especially, Type-D region revealed a third prophage (ifp3, 11,121 bp), which corresponded to the blotchy mottle symptoms and was absent in CLas infected psyllids. Hence, the roles of genes in the Type-D prophage region were investigated. Herein, we reported the cloning, overexpression, and purification of ORF1, an open reading frame from the partial Type-D region of CLas. At physiological condition, the protein existed in multiple conformations as evidenced by gel filtration chromatography and the native-page. The heterologous overexpression of ORF1 was toxic to the E. coli host cells, and the Agrobacterium tumefaciens mediated transient expression in Arabidopsis thaliana resulted in rapid and total chlorosis of the seedlings. The functions of this protein are currently under investigation. 183 The 44 th Congress on Science and Technology of Thailand (STT 44)

190 B3_009_PA: WHITE SPOT SYNDROME VIRUS ANNEXES PMO-MIR-315, SHRIMP MiRNA, TO REGULATE PROPHENOLOXIDASE SYSTEM VIA PPAE3 GENE SUPPRESSION Phattarunda Jaree 1, Chantaka Wongdontri 2, and Kunlaya Somboonwiwat 2,3* 1 Institue of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom 73107, Thailand. 2 Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10300, Thailand 3 Omics Science and Bioinformatics Center, Faculty of Science, Chulalongkorn University, Phayathai Rd., Bangkok 10300, Thailand * kunlaya.s@chula.ac.th Abstract: The microrna function in shrimp antiviral response has been reported; however, it needs more investigation. In previous work, we identified the mirna that response in WSSVinfected shrimp using next-generation sequencing technique and found pmo-mir-315 that highly expressed in shrimp hemocyte after WSSV infection. Therefore, this research focused on determination of pmo-mir-315 functions and its target mrna in shrimp antiviral immunity. Firstly, the pmo-mir-315 expression was found to be up-regulated in WSSV-infected shrimp and it was highly expressed (about 30 fold) at 48 hours post infection. The expression of pmomir-315 target mrna, putative prophenoloxidase (PO)-activating enzyme (PmPPAE3), was correlated with pmo-mir-315 induction as it was down-regulated in WSSV-infected shrimp. Therefore, pmo-mir-315 might be regulated PmPPAE3 via inhibition process. Then, the investigation of the direct interaction revealed that the pmo-mir-315 could bind to CDS of putative PmPPAE3 in vitro, which is consistent with the decrease of PmPPAE3 transcript level and PO activity. Moreover, WSSV copy number was also increased after introducing pmo-mir- 315 in WSSV infected shrimp hemocyte. Because of the novel PmPPAE, full-length of PmPPAE3 was identified and aligned with other PPAE in various species. Tissue distribution analysis revealed that PmPPAE3 gene was only expressed in shrimp hemocyte like PmPPAE1 and PmPPAE2. In PmPPAE3 knockdown shrimp, PO activity was dramatically decreased. The result showed that the PmPPAE3 involved in propo system. Taken together, the pmo-mir-315 inhibits PO activity via down-regulating PmPPAE3 expression, resulting in the reduction of WSSV replication in WSSV-infected shrimp. This is the first report of the pmo-mir-315 regulated prophenoloxidase system via inhibiting PmPPAE3 gene expression that helps WSSV propagation in WSSV-infected shrimp. 184 Science and Technology in the Disruptive Era

191 SESSION B: BIOLOGICAL SCIENCE (B4: BIOMEDICAL SCIENCE) 185 The 44 th Congress on Science and Technology of Thailand (STT 44)

192 B4_001_OF: SUPPRESSION OF AFLATOXIN B1-INDUCED INTRACELLULAR REACTIVE OXYGEN SPECIES PRODUCTION AND CYTOTOXICITY IN HEPG2 CELLS BY PURPLE WAXY CORN EXTRACTS Tichakorn Singto *1, Wongwiwat Tassaneeyakul 1, Supatra Porasupatana 1 1 Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand * thurkonnun13@gmail.com Abstract: This study was designed to determine the effects of purple waxy corn extracts (PWCES) from three parts including cob, seed and seed-boiled water on cytotoxicity and intracellular reactive oxygen species (ROS) production in aflatoxin B1 (AFB1)-treated HepG2 cells. The bioactive compounds including total phenolic content (TPC), total flavonoid content (TAC) and total anthocyanin content (TAC) of PWCES were a range from ±2.46 to ±10.62 µg Gallic acid/mg extract, 0.55±0.09 to 21.35±0.33 µg Quercetin/mg extract and 43.70±2.61 to ±3.43 mg Cyanidin-3-glucoside/L extract, respectively. Antioxidant activities determined using oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays showed a range from ±13.23 to ±33.69 µm trolox/mg extract and ±0.04 to ±0.03 µm trolox/mg extract, consecutively. Correlations between bioactive compounds in TPC, TFC and TAC and antioxidant activity were observed with correlation coefficient in a range (p<0.05). PWCES (0.5 and 1 mg/ml cob, 0.25, 0.5 and 1.0 mg/ml seed 0.5 and 1.0 mg/ml, and 0.1, 0.5 and 1.0 mg/ml seed-boiled water) significantly reduced AFB1-induced cytotoxicity in HepG2 cells (p<0.05). In addition, PWCES reduced the production of intracellular ROS in a dose-dependent manner (p<0.05) with EC50 as 0.356, and mg/ml for cob, seed and seed-boiled water, respectively. There were correlations between % inhibition of intracellular ROS production and all bioactive compounds (R= , p<0.01). In conclusion, PWCES effectively reduced intracellular ROS production cytotoxicity induced by AFB1 in HepG2 cells. 186 Science and Technology in the Disruptive Era

193 B4_002_PF: MONOPHOSPHORYL LIPID A (MPLA) ENHANCES CYTOKINE PRODUCTION AND MATURATION OF DENDRITIC CELL IN RESPONSE TO Pythium insidiosum VACCINE Asma Longkunan 1,2,*, Patimaporn Wongprompitak 2, Pattama Ekpo 2, Wiwit Tantibhedhyangkul 2 1 Graduate Program in Immunology, Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand 2 Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand * asmalongkunan@gmail.com Abstract: Pythiosis is an infection caused by Pythium insidiosum, an oomycetes (fungus-like pathogen). Nowadays, surgery is the only curative treatment for vascular form of pythiosis, which is mostly found in Thai thalassemia patients. However, the disease may relapse in some cases. Thus, pythium antigen has been used as adjunctive immunotherapy. However, the efficacy of this inactivated vaccine is inconsistent and varies among patients, probably due to the lack of adjuvant. Monophosphoryl lipid A (MPLA) has been reported to induce higher immune response to HPV vaccine. Therefore, this study aimed to evaluate the role of MPLA on dendritic cells (DCs), which are the main target of vaccines. Herein, human monocyte-derived dendritic cells were generated in vitro from adherent monocytes cultured in media with GM-CSF and IL-4. Then, cells were stimulated with pythium antigen alone or in combination with MPLA. The cytokine gene expression and DC maturation were determined by qrt-pcr and flow cytometry, respectively. We found that, vaccine with MPLA, but not vaccine alone, markedly increased the expression of cytokines, namely IL-1β, IL-6, and IL-23p19. In addition, MPLA induced the dendritic cells maturation as shown by the upregulation of CD83 (maturation marker) and CD86 (co-stimulatory molecule). In conclusion, this study demonstrates that MPLA augmented the pythium antigen-induced innate response in DCs including cytokine production and DC maturation. This may provide benefit for the development of protective immunity against this infection. However, further study is required to determine the effect of vaccine-adjuvant on the induction of antigen-specific T cell responses. 187 The 44 th Congress on Science and Technology of Thailand (STT 44)

194 B4_003_PF: A THAI CASE OF DE NOVO 18QTER DELETION SYNDROME WITH MULTIPLE CONGENITAL DEFORMATIONS AND 46,XY,del(18qter) KARYOTYPE Sasithorn Boonnoon, Thavorn Supaprom, Kannika pratoompuk and Kaew Udomsirichakorn Chromosome Analysis Center, Department of Biological Science, Faculty of Science, UbonRatchathani University, UbonRatchathani, THAILAND thavorn_s@yahoo.com Abstract: We reported on a 7 - day old Thai male baby with multiple congenital deformations such as brachycephaly, flat occiput or plagiocephaly, and ankyloglossia, bearing a terminal deletion of long arm of chromosome 18q. Standard cytogenetic analysis by GTG-banding from peripheral blood samplings was performed. The structural chromosomal abnormality of proband reveals a deletion of the terminal region of the long arm of chromosome 18, ranging from band q21 to qter. This result suggested that the proband demonstrated a 46,XY,del(18qter) karyotype. Parental chromosome analysis exhibited normal karyotype. It concluded that a deletion of the distal part of the long arm of chromosome 18 found in this case was de novo. Further study for an accuracy identification of the breakpoint in the long arm of chromosome 18 by molecular cytogenetic techniques is necessary for assessment of karyotype-phenotype correlations in this syndrome. Keywords: De novo 18qter deletion syndrome, 46,XY,del(18qter) karyotype, multiple congenital deformations, ankyloglossia 188 Science and Technology in the Disruptive Era

195 B4_004_PF: EFFECTS OF HELIUM/OXYGEN PLASMA JET ON THE SHEAR BOND STRENGTH OF SELF-ADHESIVE RESIN CEMENT TO DENTIN Pranisa Emyoo 1 *, Pavisuth Kanjantra 2, Dheerawan Boonyawan 3 1 Master of Science (Dentistry), Faculty of Dentistry, Chiang Mai University, Thailand 2 Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Thailand 3 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Thailand * tangnisa055@gmail.com Abstract: Self-adhesive resin cements have been developed to simplify the dental bonding process. However, bond strengths of self-adhesive resin cements are lower than those of resin cements used with adhesive bonding systems. Cold atmospheric plasma jets have the ability to both disinfect and improve the bonding quality of direct resin composite restorations. The effects of the plasma also depend on the plasma chemistry. This study investigated the effects of helium/oxygen plasma on shear bond strength (SBS) when RelyX U200 self-adhesive resin cement is applied to dentin, 24 hours after bonding, using the following five methods: dentin surface treated with no plasma treatment (control group); helium plasma (He); helium/oxygen plasma (He/O 2); helium plasma with rewetting (He+R); and helium/oxygen plasma with rewetting (He/O 2+R). One-way ANOVA and Sheffe Post Hoc test statistics indicated higher SBS in both groups treated with plasma with rewetting than in the plasma-only and control groups (p<0.05). Water contact angle measurements in all plasma-treated groups showed lower contact angle values, indicating the improvement of surface wettability. Scanning electron microscope (SEM) evaluations proved that plasma treatments with rewetting could partially clean the smear layer and open dentinal tubules, resulting in resin tag formation at the interfaces, and enhance the bonding quality. 189 The 44 th Congress on Science and Technology of Thailand (STT 44)

196 B4_005_PF: SEROPREVALENCE OF Leptospirosis AT COMMUNITY LEVEL IN NORTHEAST THAILAND Chan Dara 1,2*, Siriluck Anunnatsiri 3, Pattapong Kessomboon 4, Thewarach Laha 5, Banchob Sripa 6, Suppalak Brameld 7, Watcharee Saisongkorh 7, Sutas Suttiprapa 2 1 Graduate School, 2 Tropical Medicine Graduate Program (International Program), Academic Affairs, 3 Department of Medicine, 4 Department of Community Medicine, 5 Department of Parasitology, 6 Department of Pathology, Khon Kaen University, Khon Kaen 40002, Thailand 7 National Institute of Health, Department of Medical Science, Ministry of Public Health, 88/7 Tiwanon Rd. Nonthaburi 11000, Thailand * dr.chan@kkumail.com Abstract: Leptospirosis caused by leptospiral bacteria is an emerging infectious zoonotic disease that is endemic in both tropical and subtropical regions. Clinical features in human ranging from mild or asymptomatic infection to fulminant hepato-renal failure. The previous study showed high seroprevalence of leptospirosis in the northeast of Thailand in hospital level. Here, we report the seroprevalence in the community level. Sera were collected from asymptomatic people from the Khon Kaen and Kalasin provinces in the Northeast of Thailand. Immunochromatographic screening results showed overall positivity for Leptospira in 113 out of 213 (53%). Males had a significantly higher seropositivity than females (P=0.008). Serovars of Leptospira were detected by microscopic agglutination test (MAT). Of 60 positive samples examined, 5 samples were positive. Two of them were positive for multiple serovars. The detected serovars were Cynopteri (20%), Icterohaemorrhagiae (20%), Australis (20%), Shermani (20%), Autumnalis (10%), and Pomona (10%). This result indicates that seroprevalence of leptospira is still high and the serovars of Leptospira detected at hospital level and community level are different and warrant further investigation. 190 Science and Technology in the Disruptive Era

197 B4_006_PF: DEVELOPMENT OF A HEALTH CHECKUP DATA WAREHOUSE FOR COMMUNITY HEALTH DATA ANALYTICS May Soe Thu, 1 Phanee Pidetcha, 2 Prasong Khaenam, 3 Apilak Worachartcheewan, 4 Likit Preeyanon 4, * 1 Faculty of Medical Technology, Mahidol University 2 Community Healthcare Service Unit, Faculty of Medical Technology, Mahidol University 3 Center for Standardization and Product Validation, Faculty of Medical Technology, Mahidol University 4 Department of Community Medical Technology, Faculty of Medical Technology, Mahidol University * likit.pre@mahidol.edu Abstract: Health promotion is vital in maintaining community health because it aims to promote healthy behavior within the community that could lead to prevention for many diseases. Designing effective health promotion programs should be guided by health information and other related factors that are integrated in a way that is accessible for health promoters. The Faculty of Medical Technology at Mahidol University, Thailand, has been accumulating health data from a mobile health checkup service provided to people in Bangkok and nearby areas for almost a decade; however, the data have been rarely used by researchers and health promoters due to limited access to the data in the database. To allow researchers and health promoters to explore big health data effectively, an extract-load-transform pipeline (ETL) and a data warehouse have been developed to transform and store the data in a dimensional model to support complex queries of data from various dimensions. The warehouse serves as a data repository for researchers and managers to extract data for assessing community health and improve the mobile health checkup service. 191 The 44 th Congress on Science and Technology of Thailand (STT 44)

198 B4_007_PA: CONTRADICTING ROLES OF ERK1/2 IN TUMOR PROMOTING AND TUMOR SUPPRESSING ACTIVITIES OF TGF-BETA IN CHOLANGIOCARCINOMA Tuangporn Suthiphongchai 1, *, Phaijit Sritananuwat 1, Parichut Thummarati 1, Kittiya Islam 1 1 Department of Biochemistry, Faculty of Science, Mahidol University * Tuangporn.sut@mahidol.ac.th Abstract: Overexpression of transforming growth factor beta (TGF-beta) has been reported in cholangiocarcinoma (CCA) and correlates with aggressiveness of CCA. This cytokine is a cytokine plays contradictory roles in cancer. It acts as tumor suppressor by attenuating proliferation in normal epithelium and at the early stage of cancer, but functions as tumor promoter by enhancing invasion and metastasis at the later stage. Here the antiproliferative and invasive activities of TGF-beta and the role of ERK in these processes were investigated in KKU-M213, a CCA cell line. In KKU-M213, TGF-beta exhibited not only invasive promoting activity but also antiproliferative effect. It induced epithelial-mesenchymal transition process (EMT) in KKU-M213, as indicated by increase in vimentin expression and MMP-9 secretion and alteration of E-cadherin localization to cytoplasm. Concomitantly, TGF-beta stimulated ERK1/2 phosphorylation. Tumor promoting properties induced by TGF-beta, namely induction of invasion, migration, vimentin expression and MMP-9 secretion, were all attenuated upon treatment with U0126, a MEK inhibitor, which inhibits ERK1/2 phosphorylation. In contrast, co-treatment with U0126 enhanced TGF-beta antiproliferative effect. Thus, these data suggest the inhibiting ERK1/2 as a potential therapeutic strategy for CCA since it inhibits tumor promoting activity at the same time promotes tumor suppressing activity of TGF-beta. 192 Science and Technology in the Disruptive Era

199 SESSION B: BIOLOGICAL SCIENCE (B5: BIODIVERSITY) 193 The 44 th Congress on Science and Technology of Thailand (STT 44)

200 B5_001_PF: GROWTH AND SURVIVAL OF THE EPIPHYTIC CYANOLICHEN Coccocarpia palmicola IN A TROPICAL RAIN FOREST IN THAILAND Pitakchai Fuangkeaw, 1, * Bungon Wannalux, 2 Chaiwat Boonpeng, 1 Kawinnat Buaruang, 1 Kansri Boonpragob 1 1 Lichen Research Unit, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, THAILAND 2 A Somdet Phra Pinklao Arboretum, Herbology Office, Chatuchak, Bangkok 10900, THAILAND * pfuangkeaw@gmail.com Abstract: Growth and survival of lichens are essential information for sustainable utilization of lichen resource. Therefore, the main goals of this study were to observe growth and survival of the epiphytic cyanolichen Coccocarpia palmicola, and to develop a growth curve of the lichen. A total of 41 thalli having initial diameters between 0.36 and 9.28 cm were observed throughout the period of 11.3 years (October 2004 to December 2015). Almost all thalli grew continuously until reaching the maximum diameters, thereafter many of them degenerated, and some died. Growth rates of the complete thalli at their maximum diameters (GR cmp) averaged 3.34±2.53 mm/y, ranging from mm/y, whereas the degenerated thalli survived to the end of the study period had growth rates (GR est) averaged 1.37±2.52 mm/y, ranging from mm/y. The largest thallus diameter class (>4 cm) showed significantly higher growth rate than those smaller size classes. Larger thalli did not only had higher rates of growth but also live longer than the smaller ones. Consistently, the growth model revealed that the small thalli grow very slowly until the diameters reach 2 cm which lasts about 150 months. Thereafter, the exponential growth starts and reaches the steady state in approximately 600 months (50 years), of which the thallus diameter expands to 15 cm. This model is necessary for estimating thallus age in natural habitat. Most importantly, this study provided fundamental and significant information for chosen lichen thalli, during the exponential growth, for sustainable utilization. 194 Science and Technology in the Disruptive Era

201 B5_002_PF: EFFECT OF LOW LIGHT ON GROWTH AND PHOTOSYNTHESIS OF Ceratophyllum demersum IN SONGKHLA LAKE Pacharee Kaewchana, 1,2 Ponlachart Chotikarn, 2,3 Anchana Prathep, 4 Phatcharee Roekngandee, 1,2 Sutinee Sinutok 1,2,* 1 Faculty of Environmental Management, Prince of Songkla University, Songkhla, Thailand 2 Coastal Oceanography and Climate Change Research Center, Prince of Songkla University, Songkhla, Thailand 3 Marine and Coastal Resources Institute, Prince of Songkla University, Songkhla, Thailand 4 Seaweed and Seagrass Research Unit, Department of Biology, Prince of Songkla University, Songkhla, Thailand * ssutinee@gmail.com Abstract: Macrophytes play an important role in maintaining high physical and biological diversity and act as ecosystem engineers. Field surveys showed seasonal variation in abundance and distribution of submerged macrophytes. Submerged macrophytes in Songkhla Lake are exposed to low light intensity due to shading from an overgrowth of other macroalgae and phytoplankton blooms in summer, and high sedimentation rainy seasons. This study investigated the effect of low light intensity on growth and photosynthesis of Ceratophyllum demersum from Songkhla Lake. C. demersum were maintained in the aquaria in the laboratory for 60 days under three light treatments: 180, 90 and 45 mol photons m -2 s -1. Growth and photosynthetic activity (photosynthetic efficiency) were investigated. The results showed that growth rate of C. demersum decreased with decreasing light intensity ( ± , ± , ± g day -1 ). However, there were no changes in dark-adapted maximum quantum yield of Photosystem II, while light-adapted effective quantum yield increased with increasing light intensity. This study suggested that light plays a major role on photosynthesis and growth of C. demersum and shading will affect abundance and distribution of this species in Songkhla Lake. 195 The 44 th Congress on Science and Technology of Thailand (STT 44)

202 B5_003_PA: PRE-ADAPTED AGGRESSIVENESS AND OPPORTUNISM OF AN INVASIVE HOUSE GECKO, Hemidactylus frenatus Yingyod Lapwong 1, Jonathan Webb 1 and Ariya Dejtaradol 2 1 School of Life Science, Faculty of Science, University of Technology Sydney, Ultimo, NSW, Australia 2 Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand Abstract: The pre-adaptation hypothesis posits that successful invaders possess invasive traits developed prior to the introduction. We conducted field observations and laboratory experiments in native range of the invasive gecko, Hemidactylus frenatus, to determine its pre-adapted traits, focusing on aggressiveness and opportunism. We carried out field works during September October in 2017 in four localities in Thailand, including Hat Yai, Chiang Mai, Mook Island and Bulon Le Island. Field observations revealed that while the larger non-invasive congener H. platyurus successfully dominated prolific feeding grounds around light sources in urban habitats, the smaller H. frenatus, although being suppressed, could still co-occur in significant number. We captured both species from Hat Yai to observe their agonistic behaviour in laboratory, which showed that H. frenatus strongly displayed aggressive behaviours when confronting H. platyurus, while the latter, in contrast, mostly ignored the presence of the former. Presumably, H. frenatus employs aggressive behaviours to compensate its smaller size, and then be able to partially compete for resources with H. platyurus. Additionally, during 30-day period of captivity, we fed the geckos with ad libitum mealworms and recorded their daily consumptions. We found that H. frenatus consumed larger number of mealworms in the earlier days than the later days, while H. platyurus had a consistent consumption rate throughout the period. Accordingly, in comparing with H. platyurus, H. frenatus is an obvious opportunistic forager that can flexibly adjust its feeding strategy in responding to available resources. In summary, we suggested that H. frenatus has developed aggressiveness and opportunism prior to the introduction, potentially as a result of intense competition pressure in its native range. 196 Science and Technology in the Disruptive Era

203 B5_004_PF: THE POTENTIAL FOR CORAL RECOVERY BY THE CORAL RECRUITMENT PATTERNS IN MU KO CHUMPHON, THE WESTERN GULF OF THAILAND Juthamart Putthayakool, 1 * Thamasak Yeemin, 1 Makamas Sutthacheep, 1 Chainarong Ruangthong, 2 Sittiporn Pengsakun, Watchara Samsuvan, 1 Charernmee Chamchoy, 1 Laongdow Jungrak 1 1 Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand 2 Marine National Park Operation Center Chumphon 1, Department of National Park, Wildlife and Plant Conservation, Chatuchak, Bangkok 10900, Thailand * j.putthayakool@gmail.com Abstract: Abundance of coral colonies and coral recruitment are needed to be quantitatively examined for better understanding on potential coral recovery. This study investigated the coral recruitment patterns and their relationships with coral communities at Ko Ngam Noi, Ko Mattra and Ko Kula, Mu Ko Chumphon, Chumphon Province, the Western Gulf of Thailand. The live coral cover at Ko Ngam Noi and Ko Mattra was significantly higher than that at Ko Kula. The difference of coral self-seeding among the study sites in Mu Ko Chumphon was clearly observed. Ko Kula showed higher degree of coral self-seeding and diversity of coral recruits. The Acropora populations were the most dominant corals at Ko Ngam Noi (43.26%) but no their coral recruits. A long-term coral reef monitoring program should examine maintenance mechanisms, connectivity and coral recruitment in the Western Gulf of Thailand. 197 The 44 th Congress on Science and Technology of Thailand (STT 44)

204 B5_005_PF: ABUNDANCE OF THE WEDGE CLAM, Donax scortum FROM SMALL SCALE FISHERIES IN KRABI, TRANG AND SATUN PROVINCES Siriluck Rongprakhon,* Thamasak Yeemin, Makamas Sutthacheep, Charernmee Chamchoy, Wiphawan Aunkhongthong, Parichat Niyomthai, Orathep Mue-suea Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Huamark, Bangkok * Abstract: The wedge clam, Donax scortum (Linnaeus, 1758) is an economically important species and has high international market demand. This study aimed to compare abundance and size structure of D. scortum from two fishing methods, i.e. collecting in the intertidal zones during low tide and breath hold diving in the shallow subtidal zones at Hat Tungtalay, Krabi Province, Hat Pakmeng, Trang Province and Hat Bochetluk, Satun Province during The results revealed that high population densities of D. scortum were found in the intertidal zone of Hat Pakmeng and the subtidal zone of Hat Bochetluk. The most abundant sizes in the intertidal zones at Hat Pak Meng were in a range of cm while those at Hat Bochetluk and Hat Tungtalay were in a range of cm. The most abundant sizes in the shallow subtidal zone at Hat Bochetluk were in a range of cm. This study highlights the importance of ecological and fishery knowledge of D. scortum in the Andaman coast of Thailand. 198 Science and Technology in the Disruptive Era

205 B5_006_PF: CORAL RECRUITMENT PATTERNS ON SETTLEMENT PANELS IN MU KO CHUMPHON, THE WESTERN GULF OF THAILAND Wanlaya Klinthong,* Thamasak Yeemin, Makamas Sutthacheep, Watchara Samsuvan, Ratanawadee Niemsiri, Supphakarn Phoaduang, Suraphol Chunhabundit, Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand * Abstract: Coral recruitment is a key process in maintaining coral communities and in facilitating coral recovery after disturbances. Studies on coral larval supply and recruitment patterns in Mu Ko Chumphon are limited. This study aimed to quantitatively examine the recruitment patterns of corals by using settlement panel experiments at Ko Ngam Noi, Ko Kula, and Ko Rangkachieu in Mu Ko Chumphon, the Western Gulf of Thailand. The densities of coral recruits on settlement panels at Ko Ngam Noi and Ko Kula were significantly higher than that at Ko Rangkachieu. The highest diversity of coral recruits on settlement panels was recorded at Ko Kula, followed by Ko Ngam Noi and Ko Rangkachieu. Pocilloporiids were the most dominant taxa of coral recruits on settlement panel at Ko Ngam Noi and Ko Rangkachieu while Porites was the most dominant coral recruit at Ko Kula. This study provides the important baseline information to the understanding of coral recruitment patterns in Mu Ko Chumphon. Monitoring of coral recruitment in other island groups in the Western Gulf of Thailand is required. 199 The 44 th Congress on Science and Technology of Thailand (STT 44)

206 B5_007_PF: CORAL HEALTH AND COMMUNITY STRUCTURE ON THE SHALLOW REEF FLAT AT KO MATTRA, MU KO CHUMPHON, THE WESTERN OF GULF OF THAILAND Sittiporn Pengsakun*, Makamas Sutthacheep, Thamasak Yeemin, Wichin Suebpala, Watchara Samsuvan, Bancha Lawang, Akkharat Sangaroon Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, THAILAND * Abstract: Many shallow reef flats have faced various disturbances and led to changes in coral cover and species diversity. Monitoring on shallow reef flat communities in the Gulf of Thailand is very limited. This study aimed to compare coral reef composition, live coral cover, size structure and health condition of a dominant coral Porites lutea between shallow reef flat and shallow reef at Ko Mattra in Mu Ko Chumphon, Chumphon Province, the Western Gulf of Thailand. The percentage of live coral cover at the shallow reef flat was The dead coral cover at the shallow reef flat (29.44%) was much higher than that at the shallow reef (13.48%). The high tolerant coral species on the shallow reef flat were Pocillopora damicornis, Pavona decussata and Porites lutea. However, the species richness of corals in the shallow reef seems to be relatively high compared with that in the shallow reef flat. The most dominant coral on both shallow reef flat and shallow reef was P. lutea. The significant difference of colony size between shallow reef flat and shallow reef was detected. The medium and large colonies (>50 cm in diameter) of P. lutea were mostly found in the shallow reef flat while the smaller ones (<50 cm in diameter) were greatly predominant on the shallow reef. In terms of the health condition, six types of coral diseases and compromised health were detected, including pink borers, pink spots, white bands, white borers, growth anomalies and fish bites. Pink borers were mostly found on the P. lutea colonies. Comparatively, the colonies of P. lutea affected with pink borers, white borers, and white bands were mostly found in shallow reef flat while pink spots, fish bites, and growth anomalies were abundant in shallow reef. This study serves a critical data between shallow reef flats and shallow reefs supporting the establishment of coral reef conservation strategies in Thailand. 200 Science and Technology in the Disruptive Era

207 B5_008_PF: MICROPLASTICS IN THE WEDGE CLAM Donax scortum FROM HAT PAK MENG, TRANG PROVINCE Laongdow Jungrak,* Thamasak Yeemin, Makamas Sutthacheep, Wiphawan Aunkhongthong, Charernmee Chamchoy, Teerapong Prickchoopon, Parichat Niyomthai Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Huamark, Bangkok * Abstract: Microplastics are a very important global environmental problem in the oceans worldwide and cause considerable impacts on marine and coastal ecosystems as well as contamination in marine food web. The wedge clam Donax scortum is a filter-feeder and an importantly economic bivalve along the coasts of the Andaman Sea. This study aimed to analyze the abundance of microplastics in D. scortum collected from Hat Pak Meng, Trang Province. Microplastics were detected in 60% of D. scortum collected from Hat Pak Meng and the average abundance was 4.7 ± 1.20 items per individual. The highest frequency of the microplastic size was in a range of 1,001-2,000 µm. This study illustrates the importance of microplastics pollution and the mitigation measures are urgently required to mitigate the accumulation of microplastics in coastal and marine ecosystems along the Andaman coasts of Thailand. 201 The 44 th Congress on Science and Technology of Thailand (STT 44)

208 B5_009_PF: EFFECT OF NOISE ON OCCURRENCE OF BIRDS IN PUBLIC PARKS, BANGKOK Panisa Aimvijarn and Nipada Ruankaew Disyatat * Department of Biology, Faculty of Science, Chulalongkorn University * Nipada.R@chula.ac.th Abstract: Urban environments have become an increasing important part of the world s ecosystems. A typical urban characteristic is loud noise, which may have adverse effects on the fauna. This research aimed to assess effects of noise on occurrence of birds in 12 public parks in Bangkok, Thailand. In each public park, birds were counted and sound pressure level was measured. Correlation between sound level pressure and occurrence of birds was calculated with Pearson correlation. Critical noise threshold was defined by noise preference of each species. Mean values of sound pressure level ranged between 45 and 70 db(a) in all parks. Overall, 26 bird species were observed in the public parks. The lowest and highest occurrence of birds were observed on Watcharapirom Park and Khlongchan Community Park. The sound pressure level was significantly correlated with the occurrence of 7 bird species, namely, White-vented Myna (Acridotheres grandis), Common Iora (Aegithina tiphia), Coppersmith Barbet (Megalaima haemacephala), Oriental Magpie Robin (Copsychus saularis), Pied Fantail (Rhipidura javanica), Streak-eared Bulbul (Pycnonotus blanfordi) and Yellow-vented Bulbul (Pycnonotus goiavier). Species richness was negatively correlated with noise in the public parks although species showed variable responses to noise levels. Further works will incorporate park characteristics which may influence both noise and birds living in these parks. 202 Science and Technology in the Disruptive Era

209 B5_010_PF: GONADAL DEVELOPMENT OF DIFFERENT SIZE CLASSES OF THE WEDGE CLAM Donax scortum IN TRANG PROVINCE, THE ANDAMAN SEA Charernmee Chamchoy, 1 * Thamasak Yeemin 1, Makamas Sutthacheep 1, Cheewarat Printrakoon 2, Kanchalee Jongrangwitaya 2, Wiphawan Aunkhongthong 1, Parichat Niyomthai 1 1 Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Huamark, Bangkok, Thailand 2 Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand * Charernmee14@hotmail.com Abstract: The wedge clam, Donax scortum (Linnaeus 1758) is an economically important clam on sandy beaches, particularly in Trang Province the Andaman coast of Thailand but knowledge on its reproductive cycles and recruitment in Thailand is limited. This study aimed to examine gametogenesis of different size classes of D. scortum from Pak Meng Beach, Trang Province. This clam had one reproductive cycle a year with several stages of gamete development. The small clams, 3-4 cm length, exhibited immature stages while the clam length 4-7 cm developed gametes during February to June. Morphological and cytological features of differentiation between the gamete developmental stages were observed. The results suggest that the small sizes (<4 cm) of D. scortum should not be collected. This study highlights the importance of establishing protected areas for D. scortum in the Andaman Sea. 203 The 44 th Congress on Science and Technology of Thailand (STT 44)

210 B5_011_PA: BIRD DIVERSITY IN CHULABHORN DAM, CHAIYAPHUM PROVINCE. Sarun Keithmaleesatti, 1,* 1 Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen Thailand. * ksarun@kku.ac.th Abstract: Chulabhorn Dam is located between Phu Khieo Wildlife sanctuary, Chaiyaphum Province and Nam Nao National park, Phetchabun Province. Most of habitat type is Dry Evergreen forest. The field surveys conducted one time per month during December 2016 to November Sixteen order 54 family and 198 species of bird were reported in area. Furthermore, order Passeriformes about 33 Family 121 species were dominant. The seasonal statuses were classified to 6 types. One-hundred thirty-one of resident, 39 of winter visitor, 21 of passage migrant, 4 of resident and winter visitor, 2 of passage migrant and winter visitor and one resident and passage migrant were recorded. According to IUCN (2017) Cyanoptila cumatilis is a Near Threatened and 197 bird species are Least Concerned. Considering the relative abundant of bird in Chulabhorn dam,16 species of very common birds, 32 species of common birds, 56 species of moderately common bird, 47 of uncommon birds and 47 rare birds were recorded. The results presented that species diversity of birds in this study was higher than the diversity in the previous report in 2009 because of the difference of field surveys frequency. 204 Science and Technology in the Disruptive Era

211 B5_012_PF: WHITE SYNDROME IN SCLERACTINIAN CORALS FROM KO SAMSAO, MU KO ANGTHONG, THE WESTERN GULF OF THAILAND Wiphawan Aunkhongthong,* Thamasak Yeemin, Makamas Sutthacheep, Watchara Samsuvan, Sittiporn Pengsakun, Wanlaya Klinthong, Juthamart Putthayakool, Charernmee Chamchoy Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Huamark, Bangkok, Thailand * Abstract: Coral diseases are recognized as a major cause of reef degradation or delayed natural recovery process in some coral reefs. Most studies on coral disease in Thailand focused on Porites spp. This study aimed to assess the prevalence of coral diseases on a mushroom coral Fungia fungites at Ko Samsao, Mu Ko Angthong in the Western Gulf of Thailand. A total of 16 coral species were found around Ko Samsao. The West of Ko Samsao showed the highest live coral coverage (46%). The dominant corals were Porites lutea, Platygyra sinensis and Fungia fungites. Emerging white syndrome in F. fungites was clearly observed at the West of Ko Samsao. The prevalence of this syndrome was 83%. This study provides evidence of the emerging of white syndrome in F. fungites at Ko Samsao and implies the potential impacts on corals in the Gulf of Thailand. 205 The 44 th Congress on Science and Technology of Thailand (STT 44)

212 B5_013_PF: REEF FISH COMMUNITY COMPOSITION IN MU KO CHUMPHON AND MU KO ANG THONG NATIONAL PARKS, WESTERN GULF OF THAILAND Felipe Monteiro Gomes de Mattos,* Thamasak Yeemin, Marine Biology Research Group, Faculty of Science, Ramkhamhaeng University * Abstract: The conservation of reef fishes is directly related to the protection of the ecosystems they inhabit during their whole life cycle. The Mu Ko Chumphon and Mu Ko Ang Thong National Parks are two important Marine Protected Areas located in the western Gulf of Thailand, protecting tens of islands, within more than 400 km² conjoint area. The goal of the current study was to assess the reef fish community present at the coral reefs inside Mu Ko Chumphon and Mu Ko Ang Thong MPAs. In total 13 islands and 2 pinnacles were sampled by Underwater Visual Census using the belt transect method. We identified 84 species in 21 families. Of those 83 were registered in Chumphon and 31 in Ang Thong. Pomacentridae was the richest family with 20 species, and Neopomacentrus anabatoides was the dominant species representing up to 86% of total abundance. Ko Ngam Yai was the richest site with 40 species and reached the peak density of 52.5 ind/m², and Luang pinnacle was the most diverse site (H = 2.019), both located inside Mu Ko Chumphon. While Mu Ko Ang Thong held the poorest site (Ko Hindap) with 11 species, and less diverse one (Ko Sam Sao North), H = Previous surveys in Ko Tao and 2 of Chumphon Islands built extensive species list but still lacked in register 6 species herein observed. This result show how important it is to implement and maintain an extensive monitoring of the reef fish fauna in both MPAs due to their high richness and differentiation from other ichthyofaunal communities. 206 Science and Technology in the Disruptive Era

213 B5_014_PF: POTENTIAL OF ECOTOURISM DEVELOPMENT AT SOME CORAL REEFS AND UNDERWATER PINNACLES IN CHUMPHON PROVINCE Makamas Sutthacheep, 1, * Thamasak Yeemin, 1 Mathinee Yoocharoen, 2 Wasana Phantewee, 3 Wichin Suebpala 1, Charernmee Chamchoy. 1 Juthamart Putthayakool. 1 Wiphawan Aunkhongthong. 1 1 Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand 2 Marine and Coastal Resources Institute, Prince of Songkhla University, Songkhla, Thailand 3 Biology Department, Faculty of Science and Technology, Rajamangala University of Technology KrungthepUniversity, Bangkok, Thailand * smakamas@hotmail.com Abstract: Coral reef tourism is one of the important ecosystem services provided by coral reefs providing a great economic benefit to coastal and island countries worldwide. However, intensive tourism without proper management also causes adverse effects on coral reef ecosystem. Seeking new dive sites is one of the solutions that could help reduce pressures on major dive sites. In this study, we assessed the potential of ecotourism development at some coral reefs and underwater pinnacles in Chumphon province. An assessment framework and criteria were developed in which both physical and biological factors are considered. Weighing scores were given and validated by experts. Field surveys were then conducted at 23 coral reefs and 13 underwater pinnacles during February July 2018 to gather relevant information for the analysis. Based on the surveys of 36 study sites, three reef sites (Ko Ngam Noi, Ko Lak Ngam, and Ko Thalu) and an underwater pinnacle (Hin Pae) exhibited a high potential for ecotourism development. Eight reef sites (Ko Kula, Ko Rang Kachiu, Ko Kalok, Ko Ngam Yai, Ko Thonglang, Ko Lawa, Ko Mattra, and Ko Chorakhe) and three underwater pinnacles (Hin Mai, Hin Klang Ao, and Hin Haeng) were assessed as medium potential. This study provides assessment methodology and identifies coral reefs and underwater pinnacles that can be possibly promoted and developed for ecotourism sites. 207 The 44 th Congress on Science and Technology of Thailand (STT 44)

214 B5_015_PF: TOURISM CARRYING CAPACITY ASSESSMENT AT SOME DIVE SITES IN CHUMPHON PROVINCE Thamasak Yeemin, 1 * Makamas Sutthacheep, 1 Chainarong Ruangthong, 2 Sittiporn Pengsakun, 1 Wichin Suebpala, 1 Watchara Samsuvan, 1 Laongdow Jungrak, 1 Rattanawadee Niamsiri 1 1 Marine Biodiversity Research Group, Department of Biology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand 2 Chumphon Marine National Park Operation Center 1, Department of National Parks, Wildlife and Plant Conservation, Chumphon, Thailand * thamasakyeemin@yahoo.com Abstract: Carrying capacity one of the management tools for managing anthropogenic impacts on coral reefs. However, most studies on carrying capacity have focused on popular dive sites where the number of tourists in such dive sites has already exceeded the carrying capacity and when the impacts have emerged. In fact, carrying capacity can be used as a precautionary measure to prevent the tourism impacts that may be happened in the future. In this study, we aimed to assess tourism carrying capacity at some coral reefs and underwater pinnacles in Chumphon Province covering three aspects, i.e. Psychological Carrying Capacity (PsCC), Physical Carrying Capacity (PCC), and Facility Carrying Capacity (FCC). The surveys were conducted at nine reef sites (Ko Chorakhe, Lak Ngam, Ko Lawa, Ko Ngam Yai, Ko Ngam Noi, Ko Kalok, Ko Thalu, Ko Mattra, Ko Rang Kachiu) and an underwater pinnacle (Hin Pae), Chumphon Province, the Western Gulf of Thailand during February July Snorkeling has been found in most reefs sites while scuba diving has been found at Ko Ngam Noi and Hin Pae. According to our analysis, all study sites were below the carrying capacity. This study provides a baseline information on carrying capacity of some dive sites in Chumphon Province which is beneficial for further coral reef conservation and tourism management. 208 Science and Technology in the Disruptive Era

215 B5_017_PF: BIODIVERSITY OF DISCOCRUSTOSE LICHEN OF MANGROVE FOREST IN THE EASTERN COAST OF THAILAND. Phimpisa Phraphuchamnong, Kawinnart Buaruang, and Patchara Mongkolsuk Lichen Research Unit. Department of Biology. Faculty of Science. Ramkhamheang University. Huamark, Bangkapi, Bangkok 10240, Thailand * Abstract: During December 2014 to March 2016, the discocrustose lichen one thousand six hundred and sixty seven samples from 22 phorophyte and stone of mangrove forest in The Eastern coast; Chachoengsao, Chanthaburi, Chonburi, Rayong and Trat province of Thailand were compiled and taxonomically catalogued into nine families thirteen genera thirty one species. Bacidia convaxula (Müll.Arg.) Zahlbr., and Lopadium disciforme (Flot.) Kullh., are the first time to be found in Thailand. Whereas Caloplaca sp. and Ramboldia sp. are expected to be new. However the highest species diversity is Lecanora helva Stizenb. Keywords: Mangrove forest, discocrustose lichens, biodiversity, The Eastern Coast 209 The 44 th Congress on Science and Technology of Thailand (STT 44)

216 SESSION C: CHEMISTRY (C1: ANALYTICAL CHEMISTRY) 210 Science and Technology in the Disruptive Era

217 C1_001_PF: A NOVEL OPTICAL SENSOR FOR DETERMINING NITRATE IONS IN WATER USING POLYVINYLPYRROLIDONE AND ACID BLUE 22 Cholapat Varongchayakul*, Chalida Haruehansapong, Ind Tungsatitchai and Usa Jeenjenkit Department of Chemistry, Mahidol Wittayanusorn School, Nakhon Pathom, 73170, Thailand * cvarong@gmail.com Abstract: Nitrate ions come from the nitrogen cycle and are very crucial to the environment. Though important, nitrate ions can build up to high amounts, causing nitrate toxicity in organisms. Methods commercially available for determining the amount of nitrate ions in water today involve dangerous chemicals and have a short range of detection. In this research, a simple colorimetric method for determining nitrate ions in water was studied. A water soluble dye, Acid Blue 22, was investigated in a water-soluble polymer medium, polyvinylpyrrolidone (PVP). The dye will function as a sensor to indicate the amount of nitrate ions. All chemicals used in this sensor are safer than the chemicals used in other proposed methods for nitrate detection. This proposed method showed high precision and accuracy in detecting nitrate ions and linearity was achieved from 0 to 500 mg L -1 nitrate (R 2 = ). The color change from blue to colorless of this proposed sensor was significant and was visible to observe with the naked eye. This test can be used in water with a ph range of 6.50 to The 44 th Congress on Science and Technology of Thailand (STT 44)

218 C1_002_PA: SIMULTANEOUS DETERMINATION OF TRACE LEAD AND ANTIMONY IN GUNSHOT RESIDUES BY VOLTAMMETRY Saowapa Planonth 1, *, Jaroon Junsomboon 2, Jaroon Jakmunee 3 1 Crime Scene Investigation Sub-division, Police Forensic Science Center 1, Office of Police Forensic Science, Royal Thai Police, Pathumtani 12120, Thailand 2 Section of Construction Materials, Division of Engineering Materials, Department of Science Service, Ministry of Science and Technology, Bangkok 10400, Thailand 3 Laboratory for Analytical Instrumentation and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand * sao283@hotmail.com Abstract: Differential pulse anodic stripping voltammetric method has been developed for determination of lead and antimony in gunshot residue (GSR). The sample was extracted with 1.5 M HCl. An aliquot of 10 ml of the extracted solution were put in a voltammetric cell, then the solution was stirred at 2000 rpm and purged with nitrogen gas for 3 min. After that the deposition of the metals was carried out by applying a constant potential of V versus Ag/AgCl to a hanging mercury drop electrode (HMDE) for 30 s. Later, a differential pulse scanning waveform from to V was applied to record a voltammogram. Peak current of lead and antimony were measured at peak potentials of and V vs Ag/AgCl, respectively. Standard addition procedure was used for quantification. Relative standard deviation for 7 replicate determinations of 5 µgl -1 of both the metals were in the range of 1.40 and 0.80% for lead and antimony, respectively. Percentage recoveries obtained by spiking 10 µgl 1 each of lead and antimony to the sample solution were found to be 104 and 94% and with detection limit of 0.13 µgl -1 and 0.19 µgl -1, respectively. It was found that contents of lead and antimony from the gunshot residue samples were in the range of µgl -1 and < µgl -1, respectively. The developed method provides good sensitivity and selectivity. The proposed method is simple, convenient, and low reagent consumption. 212 Science and Technology in the Disruptive Era

219 C1_003_PA: DETERMINATION OF CYANIDE IN CONCRETE ROOFING TILES BY DIFFERENTIAL PULSE VOLTAMMETRIC METHOD Jaroon Junsomboon 1, *, Jaroon Jakmunee 2 1 Section of Construction Materials, Division of Engineering Materials, Department of Science Service, Ministry of Science and Technology, Bangkok 10400, Thailand 2 Laboratory for Analytical Instrumentation and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand * jjunsomboon@gmail.com, jaroon@dss.go.th, Abstract: Differential pulse cathodic voltammetric technique with a hanging mercury drop electrode (HMDE) has been developed for the determination of cyanide in a water extracted from concrete roofing tile samples. The sample was extracted with water for 24 h according to the TIS standard method. An aliquot of 5 ml of the extracted solution and 5 ml of electrolyte solution (0.2 M KOH M H 3BO 3) were put in a voltammetric cell, stirred at 2000 rpm, and purged with nitrogen gas for 3 min. Then, a constant potential of 0.00 V versus Ag/AgCl reference electrode was applied to the working electrode for 10 s; the differential pulse waveform was cathodically scanned from 0.0 to V to record a voltammogram. Reduction peak current at peak potential of V vs Ag/AgCl electrode was direct proportional to cyanide concentration. Relative standard deviation for 11 replicate determinations of 0.04 mg L -1 CN - was 2.62%. Percentage recovery obtained by spiking 0.04 mg L -1 CN - to the sample solution was found to be 95%, and a detection limit of mg L -1 was achieved. The developed method provides good sensitivity and selectivity, which is suitable for quality control of cyanide in the concrete roofing tile products, and it is simple, convenient operation, and low reagent consumption. 213 The 44 th Congress on Science and Technology of Thailand (STT 44)

220 C1_004_PA: STUDY OF THE REMOVAL EFFICIENCY ON METHYLENE BLUE UNDER VISIBLE LIGHT BY USING Cu 2O PRECIPITATION WITH ZnO CATALYST IN PHOTOCATALYSIS METHOD Patchariya Chawalitjinda 1 Advisor: Sirihathai Srikwanjai, Mahidol Wittayanusorn School, Nakhon Pathom, 73170, Thailand Mentor: Cheewita Suwanchawalit, Lecturer, Department of Chemistry, Silpakorn University, Bangkok, 10200, Thailand Students: Mahidol Wittayanusorn School, Nakhon Pathom, 73170, Thailand * patchariya114@gmail.com Abstract: Cu 2O - ZnO catalyst was synthesized by co-precipitation method for removal of methylene blue under visible light. The ph of the solution and the concentration of glucose was investigated in order to synthesize coupled complex catalyst. The catalysts were characterized by Fourier-transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray powder diffraction (XRD). The efficiency of methylene blue removal was measured by UV-visible spectrophotometer. Form the characterization, it was found a transmission peak at around 605 cm -1, which can be attributed to the Cu (I) -O vibrations, and octahedral Cu 2O shape. The results show that the highest methylene removal efficiency is 98% and the most efficiency catalyst was prepared at ph condition 14 and concentrations of glucose was 0.18 M. Figure 1 Characterization of Cu 2O catalyst (left) and Cu 2O - ZnO catalyst (right) by using Scanning Electron Microscope (SEM) (Magnifying power at x2. 5k) Keywords: Co-precipitation, Methylene Blue, Methylene Blue removal, Copper (I) Oxide 214 Science and Technology in the Disruptive Era

221 C1_005_PA: DEVELOPMENT TEST KITS FOR DETERMINATION BLOOD IN EVIDENCE AT A CRIME SCENE Udomsak Palee 1, *,Saowapa Planonth 1,, Jaroon Junsomboon 2, Jaroon Jakmunee 3 1 Crime Scene Investigation Sub-division, Police Forensic Science Center 1, Office of Police Forensic Science, Royal Thai Police, Pathumtani 12120, Thailand 2 Section of Construction Materials, Division of Engineering Materials, Department of Science Service, Ministry of Science and Technology, Bangkok 10400, Thailand 3 Laboratory for Analytical Instrumentation and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand * udomsakcup@hotmail.com Abstract: Police detectives use various scientific tools to analyze evidence at a crime scene. One of the tool is a test kit for detection the presence of blood. The test provides evidence if red spots found at a crime scene are actually blood. We have developed 2 test kits for blood detection, i.e., Phenolphthalein presumptive blood test kit (Kastle-Meyer reaction) and leucomalachite green presumptive blood test kit (Leuco Malachite Green reaction). Both methods are based on the oxidation of the dye reagents by hydrogen peroxide catalyzed by hemoglobin in blood. They were compared with commercially available test kits for presumptive tests for blood (based on Kastle-Meyer reaction). In this study, the tests were subjected to diluted blood (from 1:10 to 1:100000). Samples were tested for blood to determine whether the presumptive tests damaged. Phenolphthalein presumptive blood test kit had a sensitivity of 1:10,000 (reagents contained Part A 2% w/v Phenolthalein, Part B Ethyl alcohol 5% v/v, Part C 1% v/v Hydrogen peroxide), leucomalachite green presumptive blood test kit had a sensitivity of 1:1,000 (reagents contained Part A 0.1% w/v leuchomalachite green, Part B Ethyl alcohol 5% v/v, Part C 1% v/v Hydrogen peroxide) and commercially available test kits had a sensitivity of 1:1,000. The developed test kits are inexpensive, easy to perform, and provides quick results. Commercial test kit is 4000 baht/set (30 ml of reagent) while our test kits are 30 and 40 baht for the same amount. 215 The 44 th Congress on Science and Technology of Thailand (STT 44)

222 C1_006_PF: SIMPLE AND RAPID MEASUREMENT OF ETHANOL IN PERFUME BY PORTABLE RAMAN SCATTERING SPECTROMETER: POTENTIAL PERFUME ETHANOL POISON FOR INFANTS Nopchonsorn Manpiankit, 1* Sirinee Thiangthae, 2 Chaksawat Sangawitayakorn, 2 Noppadon Nuntawong, 3 Prapin Wilairat, 4 Rattikan Chantiwas 1,2 1 Forensic Science Unit, Medical and Graduated Education Division, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand 2 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand 3 Optical Thin Film Laboratory, National Electronics and Computer Technology Center (NECTEC), Pathumthani 12120, Thailand 4 National Doping Control Centre, Mahidol University, Rama 6 Rd., Bangkok 10400, Thailand * nopchonsorn.man@gmail.com Abstract: Perfume commonly contains ethanol as solvent of the fragrant components as well as for promoting evaporation of the fragrance. Ethanol content can vary over a wide range from 50 to 99% (v/v). Toxicity of ethanol in infant is of concern both from direct ingestion or adsorption through the infant s skin. Lethal dose of ethanol in infant by ingestion is 3g/kg infant weight. Therefore, information of the ethanol content in perfume is necessary in order for parents to be aware of the potential toxic risk of the product to their child. A rapid and convenient method for the determination of ethanol content in perfume product will be useful as an indicator of the ethanol toxicity. This work aims to apply Raman scattering technique as a rapid and direct method for measurement of the ethanol content in commercial perfume products. Gas chromatography with FID was used as the comparison method to validate the method. Linear calibration curves from the Raman scattering and GC FID methods were y = (7.7±0.1)x + (114.1±5.8) and y = (1781.1±24.7)x + (569.8±878.6), respectively, with good coefficient of determination (R 2 > 0.99). There was no statistically significant difference between the two methods (p > 0.05). The measured ethanol content in four local perfume products were in the range 66.0 to 77.0% (v/v). The Raman scattering method provides high sample throughput of 120 samples/h, employing a convenient portable Raman scattering spectrometer. 216 Science and Technology in the Disruptive Era

223 C1_007_OA: DEVELOPMENT OF COLORIMETRIC METHOD FOR SCREENING OF ALPHA- GLUCOSIDASE INHIBITORS FROM PLANT EXTRACT Lalida Chanchaem 1,2, Plaipol Dedvisitsakul 1 1, 2* and Kanchana Watla-iad 1 School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand 2 Center of Chemical Innovation for Sustainability, School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand * Kanchana.wat@mfu.ac.th Abstract: Screening of α-glucosidase inhibitory activity usually is performed for screening traditional plants used for treatment of diabetes mellitus (Type 2). In general, the colorimetric based on p-nitrophenyl α-d-glucopyranoside (pnpg) substrate is commonly used for this screening. Nevertheless, yellow color of nitrophenol (NPG) compound produced as product from enzymatic reaction of pnpg and α-glucosidase is overlapped with the color of plant extract. So, investigation of product color shifting is important. This work, β-naphthyl α-d-glucopyranoside (BNG) substrate was used for reducing the overlapping of color. Firstly, the 2-napthol liberated from enzymatic process of BNG was combined with diazonium salt. Concentration of BNG, nitrite, aniline and enzyme as well as incubation and detection time were also optimized. Then, the colored solution from the reaction was detected at 540 nm. Finally, this method will be validated with reference pnpg substrate and applied for screening half maximal inhibitory concentration (IC 50) of traditional plant extract. 217 The 44 th Congress on Science and Technology of Thailand (STT 44)

224 Current (µa) Current (µa) C1_008_PA: POLYPYRROLE-DOPED CARBON BLACK MODIFIED ON SCREEN-PRINTED CARBON ELECTRODE FOR GENISTEIN DETECTION USING IONIC LIQUID AS SUPPORTING ELECTROLYTE Surinya Traipop, 1 Orawon Chailapakul, 1 Janjira Panchompoo, 1 Eakkasit Punrat, 2 Wanida Wonsawat, 3 Suchada Chuanuwatanakul 1, * 1 Electrochemistry and Optical Spectroscopy Center of Excellent, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand 3 Department of Chemistry, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand * suchada.c@chula.ac.th Abstract: Currently, screen-printed carbon electrode (SPCE) is extensively proposed to overcome portable and disposable properties because it is easy to be fabricated on various types of substrates. Therefore, a SPCE can be conducted on a portable material; using a simple conducting method leading to lower cost. In this work, a sensitive, portable, and disposable sensor, which is a SPCE with a three-electrode system on a 0.15 mm thick polyvinyl chloride (PVC) substrate, has been developed for the genistein determination. Polypyrrole-doped carbon black was also modified on the SPCE to enhance its sensitivity. However, genistein is an organic compound normally dissolved in non-polar solvent that reduces electrical conductivity of the solution. Although polar organic solvent such as ethanol was generally selected as solvent for genistein, ionic liquid can also dissolve organic compounds with better conductivity. Hence, the comparison of solvents between ethanol and ionic liquid for the determination of genistein by electrochemical detection using the modified SPCE was studied as shown in figure 1. SCREEN-PRINTED CARBON ELECTRODE GENISTEIN OXIDATION POLYPYRROLE CARBON BLACK IN ETHANOL 0% 1% 5% IN IONIC LIQUID 0% 1% 5% Applied Potential (V) Applied Potential (V) Figure 1. Square wave voltammograms of genistein oxidation in ethanol (left) and in ionic liquid (right) on various amount of polypyrrole modified on screen-printed carbon electrodes 218 Science and Technology in the Disruptive Era

225 C1_009_PF: ELECTROCHEMICAL DETERMINATION OF GOLD IONS IN GOLD PLATING SOLUTION USING PAPER-BASED SCREEN-PRINTED GRAPHENE ELECTRODE Phichanan Duchda and Weena Siangproh* Department of Chemistry, Faculty of Science. Srinakharinwirot University. Sukhumvit 23, Wattanna, Bangkok, 10110, Thailand. * Abstract: A new electrochemical sensor using paper-based screen-printed graphene electrode (SPGE) was first developed here for the determination of gold ions in gold plating solution or gold refining waste by square wave voltammetry. The assay was based on the reduction of gold ions at graphene electrode surface. The effects of basic experimental parameters such as pulse amplitude, square wave frequency and step height on the response of the SPGE electrode were investigated and the optimal operating conditions were obtained. Under the optimal conditions, a wide linear range of ppm (y = x , R 2 =0.9989) and a low detection limit of 1 ppm (S/N=3) were well established with the proposed approach. In addition, this developed sensor provided high selectivity toward the reduction of gold ions from commonly found ions in plating solution. The results obtained indicated, as compared with those of conventional methods (AAS or ICP-OES), the proposed sensor offers the advantages of fast analysis time, simplicity, very sensitive and no requirement of complicated operational step. These finding shows the high potential to use this sensor for real time filed monitoring. 219 The 44 th Congress on Science and Technology of Thailand (STT 44)

226 C1_010_OA: DEVELOPMENT OF MICROFLUIDIC DEVICES FOR DETERMINATION OF TOTAL CHOLESTEROL IN BLOOD Suphakorn Katib 1,3, Chalermpong Saenjum 2, Plaipol Dedvisitsakul 1, and Kanchana Watla-iad 1,3* 1 School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand 2 Center of Natural Active Pharmaceutical Ingredient Research, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand 3 Center of Chemical Innovation for Sustainability, School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand * Kanchana.wat@mfu.ac.th Abstract: Microfluidic method was developed for simply and easily determination of total cholesterol in blood samples. The online separating and detecting steps were operated on the developed device. Chromatographic method used to separate complex samples and colorimetric reaction of cholesterol used for detecting part, were performed on silica gel coated on aluminum (0.5 x 7 cm). The cholesterol reaction was based on reaction of iron (III), chloride (FeCl 3) in acetic acid and sulfuric acid (H 2SO 4) to obtain red-purple complex. Color intensity was detected as RGB value by co-operation between device and mobile phone application. The device was shown good linearity as of G value. This device may use for warning and progressing of myocardial infarction, coronary heart disease, and lipid disorders diseases. 220 Science and Technology in the Disruptive Era

227 C1_011_PF: MODIFICATION OF BENTONITE FOR DYE ADSORPTION Benjamaporn Jutapad*, Panadda Tansupo Department of chemistry, Faculty of Science and Technology, Rajabhat Maha Sarakham University, Maha Sarakham, 44000, Thailand * Abstract : In this study, the modification of bentonite was carried out by three method ; (1) thermal activation (2) chemical activation (3 thermal-chemical activation. The characteristics of the modified bentonite were analyzed by Braunneur-Emmet-Teller (BET) and Fourier transform infrared spectroscopy (FTIR) for porosity and chemical structure respectively. The effect of contact time, and acid base, ph value of dye on the removal of methylene blue, MB and methyl orange, MO were investigated. The FT-IR spectra was shown the completed characteristics of modified bentonite. The result from BET was indicated that the modified bentonite, BA50 was induced specific surface area to increase. Whereas the modified bentonite,ba6 and BB100 were shown the high pore volume and pore size, cm 3 /g and nm respectively. For the optimization of activated bentonite the result of the contact time was shown the equilibium adsorption of MB and MO are 15 and 60 min respectively. Whereas the batch adsorption studies of the ph value was revealed that the equilibium of adsorption was occured at ph 6.37 for MB and 8.16 For desorption of MO. The Langmuir and Freundlich isotherm adsorption by modified bentonite, BA50 was investigated. The Freundlich isotherm was shown a higher correlation coefficient R 2 = in compare to Langmuir isotherm R 2 = Keywords: Bentonite, Dye Adsorption, Methylene blue, Methyl Orang 221 The 44 th Congress on Science and Technology of Thailand (STT 44)

228 C1_012_PA: ELECTROCHEMICAL SENSOR OF CO 2 BASED ON SURFACE MODIFIED HALLOYSITE NANOTUBE. Orrapa Cheycharoen, Juthaporn Paothong, Chaiya Prasittichai* Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. * fscicyp@ku.ac.th Abstract: Alteration of the surface by self-assembled monolayer (SAM) has emerged as the excellent strategy to tune the surface of the materials for interested applications. In this presentation, we will describe the fabrication of electrode by selective modification of the outer surface of halloysite nanotues (HNTs) with 3-(trihydroxysilyl)-1-propanesulfonic acid for CO 2 detection. This sulfonate surface functionalized HNTs has been revealed to selectively capture and release CO 2 at low temperature, allowing for the measurement of the amount of CO 2 in the ambient air by electrochemical impedance spectroscopy. We found that the proton conductivity of SAM-modified HNTs in aqueous media was dropped by 3 4 orders of magnitude upon CO 2 binding. Further measurements of cycling CO 2 adsorption desorption revealed that this modified material can be reused for several times. The results we demonstrate here offer a new path to produce a cheap yet reliable material for CO 2 electrochemical sensor. Figure 1. Nyquist plot of SIT/HNTs plate measured under CO 2 adsorption at 30 s ( ), 420 s ( ), 840 s ( ) and 1260 s ( ) and the inset: Nyquist plot of SIT/HNTs plate measured at without CO 2 adsorption 222 Science and Technology in the Disruptive Era

229 SESSION C: CHEMISTRY (C2: INORGANIC CHEMISTRY) 223 The 44 th Congress on Science and Technology of Thailand (STT 44)

230 C2_001_OA: SOLID PREMIX FOR WATER DECONTAMINATION CONSISTING OF SOLID HYDROGENPEROXIDE AND ZEROVALENT IRON Christoph Sontag, 1, * 1 University of Phayao, School of Science, Mae Kah, Phayao, Thailand * c.sontag@web.de Abstract: Zerovalent iron is a safe, inexpensive material for wastewater treatment due to its ability to undergo Fenton-like oxidation reactions with organic pollutants under aerobic conditions, especially in the presence of peroxides. In this research, a method is used to produce a solid preparation of hydrogen peroxide as crystal water of urea in combination with iron powder. In order to be effective, citric acid as catalyst is essential to make the surface of iron particles accessible. This mix could serve as an instant treatment in form of powder in water-soluble bags or in compressed forms. The activity of such a pre-mix is tested on the oxidation reaction of p-nitrophenol as example for a critical water pollutant. Figure 1. Formation of iron(ii) from zero-valent iron (ZVI) by citric acid and subsequent Fenton reaction with Urea-Hydrogen-peroxide (UHP) for oxidative decontamination of organic pollutants 224 Science and Technology in the Disruptive Era

231 C2_002_OA: PREPARATION OF ZINC-ALUMINUM LAYERED DOUBLE HYDROXIDES FOR PHOSPHATE ADSORPTION USING MICROWAVE-ASSISTED PRECIPITATION METHOD Cholaphan Deeleepojananan, 1 Soontorn Suvokhiaw, 1 Cheewita Suwanchawalit 1, * 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand * suwanchawalit_c@su.ac.th Abstract: Layered double hydroxides (LDHs) are anionic clay widely known for their ability to adsorb harmful oxyanions in wastewater, such as phosphates, chromates, and arsenates. In this research, we report a simple, inexpensive technique for preparation of zinc-aluminum (Zn-Al) LDHs using only recycling aluminum cans accompanying with microwave power. The technique involves surface modification of Al cans to aluminum hydroxides by immersing in a strong base and heating the modified Al cans in Zn(NO 3) 2 solution under acidic condition in a home-use microwave oven at 300 W, 450 W, and 600 W followed by aging for 48 hours. The samples were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectrometry (EDX), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and tested for their phosphate adsorption. The XRD pattern and FTIR spectra of all samples confirm their LDH structures perfectly. By studying the effects of heating powers, LDH samples heated at high powers show better crystalline structure along with greater phosphate adsorption efficiency than the samples prepared at lower powers. Moreover, the Al cans are easily removed from the phosphate solution, thus this technique is promising for wastewater treatment process which requires no contamination left behind. 225 The 44 th Congress on Science and Technology of Thailand (STT 44)

232 C2_003_PA: SELECTIVE NAKED-EYE SENSING FOR OXALATE USING DINUCLEAR COPPER(II) COMPLEX UNDER INDICATOR DISPLCEMENT ASSAY Nattawat Chatphuak, Chomchai Suksai* Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Burapha University, Chonburi 20131, Thailand. * Abstract: Oxalate is an important nutrient found in many plants. However, the excessive accumulation of oxalate in the body cause a variety of health disorders. Importantly, there is no enzyme available in the human body for the degradation of oxalate. Therefore, it is important to easily know the amount of oxalate uptake from daily food. In this work we report the rapid and highly selective naked-eye sensing for oxalate using indicator displacement assays (IDAs). The dinuclear copper(ii) complexes of 1,4-bis(di-2-pyridylaminomethyl)benzene (CuL1) and 1,4-bis(tripodalamine)benzene (CuL2) were synthesized and employed as metal-based IDA receptors in which bromopyrogallol red (BPG) was used as sensing indicator. It was found that only the ensemble of CuL1 and BPG showed the highly selective sensing for oxalate even in the presence of various anions due to oxalate could change the blue color of ensemble to magenta color of free BPG. In contrast to CuL2 and BPG ensemble, all of anions could not turn the color of ensemble solution. It might be expected that the ensemble formation constant of BPG with CuL2 is larger than that of CuL1. Therefore, the effect of ligand structure plays an important role to control selective sensing of oxalate in this work. 226 Science and Technology in the Disruptive Era

233 C2_004_PF: THE ROLE OF THE SIZE OF HYDROXYAPATITE NANOPARTICLE ON OSTEOBLAST CELL TOXICITY AND THEIR APPLICATION AS MIRNA CARRIER SYSTEM Natthaya Thepphanao, 1 Pirawish Limilawan, 2 Anjalee Vacharaksa, 2 Numpon Insin 1,3 * 1 Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok Thailand 2 Departmet of Microbiology, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok Thailand 3 Center of Excellence in Materials and Bio-interfaces, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, Thailand * Numpon.i@chula.ac.th Abstract: MicroRNA (mirna) treatment is a therapeutic approach to treat many diseases by control gene expression. In order to achieve the efficient way to deliver mirna into the target cells, the development of the carrier system remains challenging. In this work, different sizes of rod-like shaped hydroxyapatite (HAp) nanoparticles were synthesized using a wet chemical precipitation and hydrothermal method for use as mirna carriers. The phase compositions and functional groups of these HAp were confirmed by X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) techniques. Transmission Electron Microscopy (TEM) demonstrated that the larger nanorod HAp of nm in width and nm in length were produced via wet chemical precipitation method, while the smaller nanorod crystal of nm in width and nm in length were obtained from hydrothermal method. The effect of different particle sizes was investigated on human osteoblast cells (HmOBs). MTT assay revealed that the smaller particle size had better biocompatibility than the larger one. Furthermore, the complexation of 100 µg/ml smaller nanorod particles and mirna302a showed the highest value of mirna binding capacity and gave the mirna expression level as high as the one with Hiperfect, the commercial transfection agent as analyzed by Quantitative Real-Time Polymerase Chain Reaction (qpcr). The obtained results suggest that nanorod HAp of nm in width and nm in length can be a good carrier system for future gene therapy application. 227 The 44 th Congress on Science and Technology of Thailand (STT 44)

234 C2_005_PF: SYNTHESIS AND STABILITY STUDY ON ZnONPs WITH DIMERCAPTOSUCCINIC ACID Poonyawee Keattanong 1, Sujittra Srisung 1 and Nootcharin Wasukan 2 1 Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumwit 23, Wattana District, Bangkok 10110, Thailand 2 National Nanotechnology Center, National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand * gs @swu.ac.th Abstract: Zinc Oxide nanoparticles (ZnONPs) has a several properties and used as a component of consumer goods especially, skincare and cosmetic products with many applications. An increasing in the amounts of ZnONPs commercial products cause risks of free radicals or reactive oxygen species (ROS) in the body, leading to oxidative stress and the cancer process in the end. meso-2,3-dimercaptosuccinic acid (DMSA) is one of the efficiency chelating reagents for treating the toxicity of several heavy meals due to DMSA is rapidly absorbed at stomach, get rid of by kidney and rare side effect. In the present work, we mainly focus on ZnONPs and DMSA-ZnONPs synthesized by cost effective and simple co-precipitation method. Then, characterized by Scanning Electron Microscopy (SEM), FTIR (Fourier Transform Infrared Spectroscopy) and UV-Vis spectrophotometry. Moreover, the stability study of DMSA complex with ZnONPs and other heavy metal were determined by UV-Vis titration and evaluated the stability constant (K) by benesi hildebrand equation. These results indicate the efficacy of DMSA for ZnONPs and heavy metals poisoning and to develop a method for treating patients with toxicity from heavy metals. 228 Science and Technology in the Disruptive Era

235 SESSION C: CHEMISTRY (C3: ORGANIC & MEDICINAL CHEMISTRY) 229 The 44 th Congress on Science and Technology of Thailand (STT 44)

236 C3_001_OA: CHEMICAL CONSTITUENTS FROM TWIGS AND LEAVES OF Goniothalamus cheliensis Wuttichai Jaidee, 1,2, * Raymond J. Andersen, 3,4 Brian O. Patrick, 3 Stephen G. Pyne, 4 Chatchai Muanprasat, 6,7 Suparerk Borwornpinyo, 7 Surat Laphookhieo 1,2 1 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Chiang Rai 57100, Thailand 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand 3 Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1 4 School of Chemistry, University of Wollongong, Wollongong, New South Wales 2522, Australia 5 Department of Physiology, Faculty of Science, Mahidol University, Rajathevi, Bangkok 10400, Thailand 6 Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Rajathevi, Bangkok 10400, Thailand 7 Department of Biotechnology, Faculty of Science, Mahidol University, Rajathevi, Bangkok 10400, Thailand * j.wutichai035@gmail.com Abstract: A phytochemical investigation on the twigs and leaves of Goniothalamus cheliensis (Annonaceae) led to the isolation of 44 compounds, of which eight were new compounds (alkaloids, styryllactones, lignans, phenolic, amides, and flavonoids). Their structures were characterized by spectroscopic methods. The absolute configurations of compounds 6 and 7 were determined from single crystal X-ray analysis using Cu Kα radiation. The absolute configurations of the other related compounds were identified by the comparison of ECD spectra with those related known compounds. Most of the isolated compounds were evaluated for their cytotoxicity against human colon cancer cell lines (HCT-116). 230 Science and Technology in the Disruptive Era

237 C3_002_OA: CHEMICAL CONSTITUENTS FROM THE LEAF AND TWIG EXTRACTS OF Desmos cochinchinensis Pornphimol Meesakul, 1,2,* Christopher Richardson, 3 Stephen G. Pyne, 3 Surat Laphookhieo 1,2 1 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Tasud, Muang, Chiang Rai 57100, Thailand 2 School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand 3 School of Chemistry, University of Wollongong, Wollongong, New South Wales 2522, Australia * pornphimon.mee13@lamduan.mfu.ac.th Abstract: The phytochemical investigation of the leaf and twig extracts of Desmos cochinchinensis led to the isolation and identification of twenty-four compounds including ten new compounds, two flavones (1 and 2), two flavanones (3 and 4), one benzyl benzoate derivative (5) and five oxepinones (6 10) together with 14 known compounds (11 24). Their structures were elucidated by spectroscopic methods. The absolute configurations of all new compounds were determined by comparisons of their CD spectra with those reported in the literature. Most of the isolated compounds were evaluated for their α-glucosidase inhibitory activity. 231 The 44 th Congress on Science and Technology of Thailand (STT 44)

238 C3_003_OF: DIRECT C H OXIDATION OF 2,4-DIAMINO-6-TRIFLUOROMETHYL PYRIMIDINE WITH PHTHALOYL PEROXIDE Natthakorn Uppatam, 1 Thitiphong Khamkhenshorngphanuch, 2 Tanatorn Khotavivattana, 3 Tirayut Vilaivan, 1 Worawan Bhanthumnavin 1, * 1 Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Department of General Education, Faculty of Science and Health Technology, Navamindradhiraj University, Bangkok 10300, Thailand 3 Center of Excellence in Natural Products Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * worawan.b@chula.ac.th Abstract: 2,4-Diamino-5-hydroxypyrimidine is an important precursor for synthesis of drugs and agrochemicals. Although direct oxidation of aromatic compounds has been widely explored, the oxidation of 2,4-diaminopyrimidine substrates bearing electron withdrawing groups was found to be extremely challenging. Herein, we report the direct C H oxidation of 2,4-diaminopyrimidine. The electron-deficient 6-trifluoromethyl substituted analogue was selected as a model compound to investigate the most suitable oxidation conditions, since the trifluoromethyl group facilitates reaction monitoring using 19 F NMR technique. The trifluoromethyl pyrimidine substrate was treated with various oxidizing agents under many different conditions. Among several oxidizing agents investigated, phthaloyl peroxide in acetic acid was the most effective reagent which afforded up to 21% of the desired product. The optimized condition was successfully reproduced at larger scale, giving the hydroxy product up to 18% isolated yield. One of the major by-products, an N-oxide species, was also isolated and fully characterized. 232 Science and Technology in the Disruptive Era

239 C3_004_PF: RELATIVE KINETIC REACTIVITY OF ALKENES, ALLENES, AND ALKYNES IN THERMAL DIELS ALDER REACTIONS Jaturavit Nimnuan and Torsak Luanphaisarnnont* Department of Chemistry and Center of Excellence for Innovation in Chemistry (PERCH-CIC) Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand * Abstract: Electron-deficient alkenes, allenes, and alkynes have been widely used as successful dienophiles in the Diels Alder reactions for total syntheses of biologically active natural products. Despite their extensive use in the reactions, a comparison of the kinetic reactivity of such unsaturated functional groups is not fully studied. This work investigates relative kinetic rates in a thermal intermolecular Diels Alder reaction of allenes, alkenes, and alkynes, using both a competition-experiment method and a comparison of absolute kinetic rates from a pseudo first-order method. The relative order of the kinetic reactivity in the Diels Alder reaction from highest to lowest was found to be alkenes, allenes, and alkynes, respectively. 233 The 44 th Congress on Science and Technology of Thailand (STT 44)

240 C3_005_PF: SYNTHESIS AND CYTOTOXICITY OF HALOGENATED CHALCONE-BASED PYRAZOLINE DERIVATIVES Dinh Phuoc Nguyen, 1 Duy Nguyen Vu, 1 Piyanuch Wonganan, 2 Warinthorn Chavasiri 1 * 1 Center of Excellence in Natural Products, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. 2 Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. * halephuoc@gmail.com, warinthorn.c@chula.ac.th Abstract: A series of new 1,3,5-trisubstituted pyrazoline derivatives have been synthesized from chalcones and acylhydrazides in two steps. In the first step, chalcones were prepared by treatment of various acetophenones with different substituted benzaldehydes by Claisen-Schmidt condensation. In the second step, pyrazoline derivatives were synthesized by conducting the reaction of chalcones with acylhydrazides in acetonitrile at 60 o C in the presence of triazabicyclodecene as a catalyst. The synthesized compounds were analyzed by elemental analyses, 1 H, 13 C NMR and MS spectral data and were evaluated for their anticancer activity. In vitro assay against colon cancer cell line (HCT-116) indicated that several compounds exhibited cytotoxicity in micromolar range, depending on substitution patterns of either 3,5-diaryl rings or aromatic ring of the 4,5-dihydropyrazole core. Among compounds screened against HCT-116 and A549 cell lines, PN002 showed potent activity against HCT-116 but inactive toward A Science and Technology in the Disruptive Era

241 C3_006_PF: SYNTHESIS AND CYTOTOXICITY OF MANSONONE G DERIVATIVES Nhung Ngo Thi Tuyet 1, Truc Phan Thi Hong 1, Rita Hairani 1, Kanphirom Lertbumroongchai 1, Piyanuch Wonganan 2, Warinthorn Chavasiri 1,* 1 Center of Excellence in Natural Products, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. 2 Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. * tuyetnhungsph@gmail.com, warinthorn.c@chula.ac.th Abstract: Mansonone G (1) is a major compound isolated from the CH 2Cl 2 extract of Mansonia gagei Drumm. heartwoods. Eight ether derivatives of mansonone G (1a 1h) were synthesized and characterized by 1 H, 13 C NMR and HRMS. All compounds were new and evaluated for their cytotoxicity against HCT-116 and HT-29 colon cancer cell lines. Interestingly, the analogues exhibited higher cytotoxicity than the parent compound, mansonone G. The most active candidate against both tested cell lines was the ether derivative 1d which displayed the IC 50 values of 2.77 and 3.24 M for HCT-116 and HT-29 cell line, respectively. 235 The 44 th Congress on Science and Technology of Thailand (STT 44)

242 C3_007_PF: NITROAROMATIC SENSORS FROM PYRENYL QUINOLINE DERIVATIVES Chantipa Lerswipapat, Mongkol Sukwattanasinitt, Paitoon Rashatasakhon* Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * Abstract: Three derivatives of pyrenyl quinolines are designed and synthesized from aniline via a 4-steps synthetic process. These compounds are different in the substitution pattern and the number of pyrene groups (Figure 1). The synthesis relies on the alkylation of aniline with propagyl bromide, the Sonogashira coupling with an aryl halide, the iodine-mediated quinoline ring formation, and the Suzuki coupling with an aryl boronic acid. The overall yields after 4 steps are approximately 10 to 20% yield. The absorption maxima of these quinoline derivatives are around 350 nm, and their molar extinction coefficients are dependent on the number of pyrene units. In acetonitrile, these compounds exhibit patternless emission bands around 450 nm, with quantum yields ranging between 40 to 62%. The preliminary screening reveals that these quinoline derivatives show selective fluorescence quenching by picric acid (PA). The poor solubility of these compounds in water and their strongly emissive nature in solid states could lead to a development of solid phase sensors for nitroaromatics. Figure 1. Structures of pyrenyl quinolines Science and Technology in the Disruptive Era

243 C3_008_PA: INVESTIGATION OF ANTI-INFLAMMATION PROPERTIES IN PIPERAZINE-2,5-DIONE DERIVATIVE Sucharat Sanongkiet, 1 Kanok-on Rayanil, 1 Jitnapa Sirirak, 1 Mary Sriwisade *,1 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 15000, Thailand. * sriwisade.m@gmail.com Abstract: Piperazine-2,5-dione (LD) is an interesting core molecule for creating the innovative drug in medicinal research. In this work, five piperazine-2,5-dione derivatives (LD1, LD2, LD3, LD7 and LD8) were synthesized and determined for their anti-inflammatory activity. There are many proteins which are involved in this process such as signaling proteins liked human canabenoid-1 receptor (CB1 receptor) and enzymes, for example, cyclooxygenase (COX) and lipoxygenase (LOX). Therefore, we tested anti-inflammation of all LD molecules by using Griess s reaction analysis and Lipoxygenase Inhibitor Screening Assay Kit (Caymann, item no ). Then, molecular docking was performed to verify the stability and specificity binding between LD molecules and CB1 receptor and LOX. According to the results from assay and molecular docking, all of LD samples did not have affinity and stability to inhibit LOX. However, they gave good affinity and stability interaction with CB1. Moreover, three LD molecules demonstrated good efficiency to inhibit nitric oxide production determined by using Griess reaction. From cytotoxicity test by MTT assay, all LD samples exhibited good anti-inflammatory activity with the IC 50 values in the range of µm. Figure 1. Synthetic scheme of five LDs. Reaction conditions: (A) Ac 2O, C, 7 h; (B) Isobutylaldehyde, t BuOK- t BuOH/DMF; (C) CH 3I, NaH/DMF 237 The 44 th Congress on Science and Technology of Thailand (STT 44)

244 C3_009_PA: MEROTERPENOIDS FROM THE LEAVES OF Psidium guajava Parichat Thepthong 1, *, Wilawan Mahabusarakam 2 1 Department of Chemistry, Faculty of Science, Thaksin University, Phattalung 93210, Thailand 2 Department of Chemistry, Faculty of Science and Natural Product Research Centre of Excellence, Prince of Songkla University, Songkhla 90112, Thailand * tsu_parichat@hotmail.com Abstract: Psidium guajava Linn. (Myrtaceae), commonly known as Farang keenok, is widespread in Thailand. Its leaves are used in Thai folk medicines as an anti-inflammatory agent and for treating wounds, pain, vomiting and diarrhea. P. guajava has been reported to contain meroterpenoids and some of them showed strong anticancer and antibacterial activities. With the aim of searching for bioactive compounds, we are therefore interested in the isolation of meroterpenoids from the leaves of P. guajava. The CH 2Cl 2 crude extract of P. guajava was purified by chromatographic method to obtain three meroterpenoids. Their structures were elucidated by spectroscopic methods and by comparison of data with those previously reported. The compounds were identified as psidial A (1), guajadial (2) and (+)-psiguadial B (3). OHC HO OH CHO O H CH 3 H H 3 C H CH 3 OHC HO OH CHO O H H H 3 C CH 3 H CH 3 OHC HO OH CHO O H CH 3 H H H3 C CH Science and Technology in the Disruptive Era

245 C3_010_PA: ONE-POT SYNTHESIS OF 2,3-UNSATURATED-O-GLYCOSYL TRIAZOLES DERIVATIVES BY FERRIC CHLORIDE AS CATALYST Jennarong Lungthaisong, Natthiya Saehlim, Rungnapha Saeeng* Department of Chemistry and Center for Innovation in Chemistry,Faculty of Science, Burapha University, Thailand * Abstract: An efficient and convenient synthesis of 2,3-unsaturated-O-glycosyl triazole derivatives has been achieved via one-pot three step reactions. The reaction employed 3,4,6-tri-O-acetyl-D-glucal, propargyl alcohol, sodium azide and alkyl bromide as substrates to carry out the three sequential reactions including glycosylation, azidation and copper-catalyzed azide-alkyne cycloaddition (CuAAC). In the presence of FeCl 3 as a catalyst, the reaction was proceeded smoothly to give a variety of target products with high -anomeric selectivity. Twenty analogues of O-glycosyl triazole adducts were obtained in good to excellent yields, proving the generality of this one pot reaction. Figure 1. One-pot synthesis of 2,3-unsaturated-O-glycosyl triazole derivatives 239 The 44 th Congress on Science and Technology of Thailand (STT 44)

246 C3_011_PA: CLERODANE DITERPENES FROM THE TWIGS OF Enicosanthum membranifolium Isaraporn Polbuppha,* Wisanu Maneerat, Surat Laphookhieo Center of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand * Abstract: Four diterpenes were isolated from the twigs of Enicosanthum membranifolium (Annonaceae). The structures were characterized as (+)-hardwickiic acid (1), 2-β-hydroxyhardwickiic acid (2), 2-β-acetoxyhardwickiic acid (3) and 15-methoxypatagonic acid (4) by spectroscopic methods and comparison of NMR spectroscopic data with those reported in the literature. 240 Science and Technology in the Disruptive Era

247 C3_012_PA: CHEMICAL CONSTITUTENTS FROM Desmos dumosus LEAVES Virayu Suthiphasilp, * Wisanu Maneerat, Surat Laphookhieo Center of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Tasud, Muang, Chiang Rai 57100, Thailand * Virayu.suthiphasilp@gmail.com, @lamduan.mfu.ac.th Abstract: The phytochemical investigation of the leaf extract of Desmos dumosus resulted in the isolation of ten known compounds, including three flavanones (1-3), two chalcones (4 and 5), a flavone (6), a aristolactam alkaloid (7), a coumarin (8) and two steriods (9 and 10). Their structures were characterized by spectroscopic methods. 241 The 44 th Congress on Science and Technology of Thailand (STT 44)

248 SESSION C: CHEMISTRY (C4: PHYSICAL & THEORETICAL CHEMISTRY) 242 Science and Technology in the Disruptive Era

249 C4_001_PA: RAPID REMOVAL OF TETRACYCLINE ANTIBIOTICS FORM AQUEOUS SOLUTION BY USING LAYERED DOUBLE HYDROXIDE COMPONENT Kwanjira Phanplado, Supalax Srijaranai, and Sira Sansuk * Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand * sirisan@kku.ac.th Abstract: Tetracycline (TC) is one group of broad-spectrum antibiotics that can be found in surface water as they are widely used in a variety of animal livestock in order to promote the growth and kill bacteria. Its contamination is a global concern since they can be harmful to ecosystem and human life. This work describes a removal approach based on an in situ co-precipitation formation of mixed metal hydroxides (MMHs) for capturing TC from aqueous solution. By employing layered double hydroxides (LDHs) components including Mg 2+ and Al 3+ ions in alkaline condition, almost 100% removal of TC was obtained within 4 min of removal time. The effect of all components on removal efficiency was investigated. The simultaneous removal of TC possibly resulted from a fast adsorption of TC molecules onto the charged surface of MMHs via hydrogen bonding and electrostatically induced attraction. Unlike the sorbentbased techniques, the proposed method was simple, rapid, efficient, and environmentally friendly since the removal can be achieved without the preparation of sorbent. 243 The 44 th Congress on Science and Technology of Thailand (STT 44)

250 C4_002_PF: THE EFFECTS OF ZEOLITE FRAMEWORKS ON ADSORPTION THERMODYNAMIC OF 1-BUTANOL IN THETA-1 AND BETA ZEOLITES Jitwadee Wiangngean, Pipat Khongpracha, Jakkapan Sirijaraensre, Piboon Pantu* Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand Center for Advanced Studies in Nanotechnology for Chemical, Food and Agricultural Industries, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand * Abstract: The two-layer ONIOM quantum cluster calculations have been employed to theoretically investigate the interactions of the adsorbed 1-butanol in Theta-1 and Beta zeolites. A 1-butanol molecule is physisorbed in Theta-1 and Beta zeolites with the adsorption enthalpies of and kcal/mol, respectively. If another 1-butanol molecule is co-adsorbed, the protonated adsorption complex is formed and the adsorption enthalpies markedly increase to and kcal/mol in Theta-1 and Beta zeolites, respectively. However, the bimolecular adsorption accompanies with a high entropy lost and becomes less thermodynamically favorable when the temperature increases in Beta zeolite. Unlike in Beta zeolite, the narrower pore walls of Theta-1 zeolite provide stronger adsorption interactions and higher adsorption enthalpies which prevail the effect of the entropy lost. The adsorbed protonated dimer is found to be the most abundant adsorbed species in Theta-1 zeolite throughout the temperature range of K. Therefore, zeolite pore confinements are very important to the adsorption by providing higher adsorption energies and differentiating preferred adsorbed species which may lead to different reaction pathways inside different zeolite structures. 244 Science and Technology in the Disruptive Era

251 C4_003_PA: DEVELOPMENT OF PAPER-BASED SENSOR WITH PORTABLE DEVICE FOR REAL TIME FIELD WORK DETECTION OF ARSENIC I WATER RESOURCES BY ELECTROCHEMICAL METHOD Boonsita Tongtokit 1, Foyfon Sensong 1, Nawan Pornananrat 1 * 1 Mahidol Wittayanusorn School, Nakhon Pathom, 73170, Thailand. 2 Department of Chemistry, Faculty of Medical Technology, Mahidol University, Nakhorn Pathom, 73170, Thailand. * lomaboonsita@hotmail.com Abstract: In some water resources, the concentration of contaminated arsenic (As) exceeds the maximum permission level at 10 μg/l of the World Health Organization (WHO). As the standard methods which normally be employed for the determination of metal quantities require the specialists to operate the instruments, the more convenient method was therefore developed in this study. This work presents the paper-based sensor based on electrochemical method by using anodic stripping voltammetry (ASV) technique. This method is easy to use and provide high sensitivity. The current peaks and applied potential were observed to measure the amount of As(V) in the samples. It was found that the square wave voltammetry (SWV) with deposition potential at 0.1 V is the most suitable conditions for As(V) detection. The Working Electrode (WE) and the Counter Electrode (CE) were screen coated by the graphene ink whereas the Reference Electrode (RE) was screen coated by Ag / AgCl. In addition, gold ion (Au+) was modified on the surface of the electrode to enhance the efficiency of the paper-based sensor. The difference of current between the background and the arsenic solution use to create the calibration curve of this experiment, which can use for calculating unknown concentration in water resources. Furthermore, paper-based sensor can be connected to the portable device and the laptop computer for the real time fieldwork detection. 245 The 44 th Congress on Science and Technology of Thailand (STT 44)

252 C4_004_OA: STUDY OF THE SYMMETRICAL PINCER PALLADACYCLE FORMATION REACTION THROUGH DILIGATION: DFT CALCULATION Siriwat Shiraarnon, Sarote Boonseng * Department of Chemistry, Mahidol Wittayanusorn School, Salaya, Phutthamonthon, Nakhon Pathom, 73170, Thailand * saroteboonseng@gmail.com Abstract: Symmetrical pincer palladacycles (PdYCY, Y is a ligand arm) are organic compounds which have been in widespread use in organic reactions. These complexes have been used particularly as catalysts or precatalysts in organic synthesis such as Heck reaction aldol condensation, and Suzuki-Miyaura reaction. Synthetic methods for PdYCY have been interesting, while the formation reaction of PdYCY has not been clear. Therefore, in this study, three symmetrical pincer complexes, i.e. [ClPd{2,6-(Me 2NCH 2) 2C 6H 3}], [ClPd{2,6-(MeSCH 2) 2C 6H 3}] and [ClPd{2,6-(Me 2PCH 2) 2C 6H 3}], were studied in terms of their reaction mechanisms. The geometries and transition structures were determined by B97XD/ G(2df,2p)[SDD]//PBE/6-31+G(d,p)[SDD] levels of theory, where [SDD] is the ECP used for the metal. The calculation results show that Gibbs free energies of PdYCY formation reactions: pincer ligand + PdCl 2 pincer palladacycle + HCl, are spontaneous. The mechanisms of PdYCY formations prefer the reaction via diligation, which is the palladium center ligated to both donor atoms in ligand arms and C-H bond activation takes place, compared to monoligation in previous work. 246 Science and Technology in the Disruptive Era

253 C4_005_PA: THE ROLE OF Ni(111) FOR PROPYLENE PRODUCTION VIA PRORPANE DEHYDROGENATION PROCESS Tinnakorn Saelee 1, Supawadee Namuangruk 2, Nawee Kungwana 1,*, Anchalee Junkaewb 2,* 1 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 2 National Nanotechnology Center (NANOTEC), National Science and Technology * anchalee@nanotec.or.th, naweekung@gmail.com Abstract: Propylene, the important raw material for a variety of products such as propylene oxide, acrylonitrile and alcohols, can be produced via propane dehydrogenation (PDH) using high effective catalyst. To date, platinum- (Pt-) based catalyst is widely used for PDH process. Due to the high reactivity of alkane reaction on Ni catalyst and good stability at high temperatures as well as lower price than Pt-based catalyst, the Ni is a promising candidate for PDH reaction. However, the reaction mechanism of PDH on Ni catalyst is still unclear. In this study, mechanisms of PDH and side reactions are theoretically investigated on Ni(111) surface. For PDH reaction, the dehydrogenation of propane to form 1-propyl intermediate before undergoing to propylene formation is more feasible than that of 2-propyl because of more kinetic favorable of PDH reaction and more thermodynamic stable of 1-proply on Ni(111) surface. Moreover, the side reaction of C-C bond cracking cannot be taken place during PDH process. Understanding of the reaction mechanisms of PDH and side reactions on Ni(111) surface is useful for designing and developing the better selective Ni catalyst by increasing the reactivity of propylene desorption and inhibiting the side reaction of C-C bond cracking and deep dehydrogenation. Figure 1. Proposed mechanisms of PDH and side reactions on Ni(111) surface 247 The 44 th Congress on Science and Technology of Thailand (STT 44)

254 C4_006_PA: EXTENDING POLYCALICENE CHEMICAL SPACE Thawalrat Ratanadachanakin, 1, * Willard E. Collier 2, * 1 Faculty of Science, Maejo University, Sansai, Chiang Mai, 50290, Thailand 2 Department of Chemistry, College of Arts and Sciences, Tuskegee University, Tuskegee, AL, 36088, USA * thawalrat4@gmail.com, wcollier@tuskegee.edu Abstract: Calicene 1 has never been synthesized, but a few polycalicenes are known. We are interested in the numerous polycalicene families resulting from different bonding motifs between the calicene monomers. Some of these polycalicene families are non-benzenoid aromatic compounds that might possess unique electronic and magnetic properties. Our earlier investigation of polycalicene chemical space has included small planar polycalicenes, belted[n]bicalicenes, band[n]calicenes, and poly-2,7-[n]calicenes. Recently we have investigated the structure and electronic properties (with a special emphasis on aromaticity) of novel polycalicenes 2-4. The aromaticities of polycalicenes 2-4 were evaluated using Schleyer s nucleus independent chemical shifts (NICS) and NMR chemical shifts. The NICS values and NMR chemical shifts of calicene, benzene, cyclopentadienyl anion, and cyclopropenyl cation were calculated for references. All geometries and NMR properties are reported at B3LYP/6-31+g(d,p) level of theory. We will discuss the structures and aromaticites of the novel polycalicenes 2-4. Figure Science and Technology in the Disruptive Era

255 C4_007_OA: PENETRATION OF ALPHA-MANGOSTIN INTO THE POPC MEMBRANE: A MOLECULAR DYNAMICS SIMULATION STUDY Wiparat Hotarat, 1 Thanyada Rungrotmongkol, 2 Supot Hannongbua 1, * 1 Center of excellence in Computational Chemistry (CECC), Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Ph.D. Program in Bioinformatics and Computational Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * supot.h@chula.ac.th Abstract: Alpha-mangostin (MGS) is commonly used to treat skin inflections, diarrhea, and chronic wounds. However, their poor solubility and stability led to limitations to use in pharmaceutical applications. Therefore, the complex formation with cyclodextrins (CDs) were used to enhance solubility and bioavailability properties of MGS. Although, the complex formation of MGS with CDs can enhance their solubility, the releasing of MGS into the membrane is somewhat lacking. Hence, we aim to study the releasing behavior of MGS from hydrophobic pocket of -cyclodextrin ( CD) and 2,6-dimethyl- -cyclodextrin (DM CD) into the 1-dipalmitoyl-2-oleyl-sn-glycero-3-phosphatidylcholine (POPC) membrane based on molecular dynamics simulation. From over a few hundred nanoseconds, an inclusion complexes of MGS and DM CD (MGS/DM CD) can adsorb on the polar head groups of POPC membrane via the hydrogen bond formation. Moreover, the MGS can spontaneously release from hydrophobic cavity of DM CD and penetrate into the lipid bilayer membrane. In case of native CD, the MGS cannot spontaneously release into the POPC membrane. Therefore, the umbrella sampling method was further applied into both systems to investigate the delivery of MGS. Finally, the release process of free form of MGS was discussed and compared with inclusion form in different CDs. Figure 1. The releasing of MGS from hydrophobic pocket of DM CD into POPC membrane. 249 The 44 th Congress on Science and Technology of Thailand (STT 44)

256 C4_008_PA; STRUCTURE AND DYNAMICS OF SERINE HYDROXYMETHYLTRANSFERASE WITH PYRIDOXAL PHOSPHATE AND TETRAHYDROFOLATE BOUND BY MOLECULAR DYNAMICS SIMULATION Peerapong Wongpituk 1, Pitchayathida Mee-udorn 2, Penchit Chitnumsub 3, Somchart Maenpuen 4, Pimchai Chaiyen 5, Supot Hannongbua 1 and Thanyada Rungrotmongkol 2,6,C 1 Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Bioinformatics and Computational Biology Progrm, Graduate School, Chulalongkorn University, Bangkok, Thailand 3 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok, Thailand 4 Department of Biochemistry, Faculty of Science, Burapha University, Chonburi, Thailand 5 Department of Biomolecular Science and Engineering, School of Biomolecular Science & Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Rayong, Thailand 6 Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand * t.rungrotmongkol@gmail.com; Fax: ; Tel Abstract: Serine hydroxymethyltransferase (SHMT), a pyridoxal phosphate (PLP; vitamin B6)- dependent enzyme, plays an important role in cellular one carbon pathways by catalyzing the reversible, simultaneous conversions of L-serine to glycine and tetrahydrofolate (THF) to 5,10-methylenetetrahydrofolate (5,10-CH 2-THF), a requisite precursor for DNA/RNA biosynthesis. In order to understand the structure and dynamics properties of human cytosolic SHMT in wild-type and Δflap variant, the SHMT and its complexes, SHMT/PLP and SHMT/PLP/THF, were performed by molecular dynamics simulation for 100-ns using AMBER16. The obtained results in terms of RMSD, RMSF and B-factor suggested that the protein structure of SHMT in apo form is more flexible than that of the complexes. The Δflap variant decreases the structure stability with a higher protein fluctuation. Among all six systems, the wild type SHMT/PLP/THF complex shows the most structure integrity and stabilization, however the flap motif fully solvated by waters has relatively high flexibility than the rest of protein. 250 Science and Technology in the Disruptive Era

257 SESSION D: POLYMER & MATERIALS SCIENCE / NANOTECHNOLOGY 251 The 44 th Congress on Science and Technology of Thailand (STT 44)

258 D_001_OF: DEVELOPMENT OF BIO-BASED COMPATIBILIZED PLA/NR BLENDS AND DIY MINI-EXTRUDER FOR FABRICATION OF 3D PRINTING FILAMENTS Pattiya Pibulchinda * Kamnoetvidya Science Academy, Rayong 21210, Thailand * p.pibulchinda@gmail.com Abstract: 3D printing is an innovative technique that is applicable to many fields. Biodegradable polylactic acid (PLA) is widely used as filament in 3D printing but its properties, such as its high brittleness, poor crystallization and cost, make it unsuitable for making commercial products. Herein our project studies way to minimize the production cost of 3D printing filament as well as ways to improve the toughness of PLA. We made do it yourself (DIY) filaments containing PLA and natural rubber (NR) from PLA: NR blends with 90:10, 85:15, and 80:20 weight compositions. In addition, compatibilized PLA/NR blends were prepared. PLA/NR blends were extruded using the GreenHero Mini-Extruder that has been designed and assembled by our team to produce filament (1.75 mm in diameter). From characterizations, SEM images show that the NR phase was dispersed in PLA as an immiscible characteristic. Maleic anhydride (MA) was employed to enhance the compatibility of NR in PLA matrix, which may improve toughness of the PLA/NR filaments. For mechanical properties, the elongation at break of PLA90/NR10 filament showed the greatest improvement compared to neat PLA. The PLA/NR blend filaments were then used in a fused deposition modeling (FDM) 3D printer to fabricate prototype samples. 252 Science and Technology in the Disruptive Era

259 D_002_PF: CORN STARCH FILM REINFORCED WITH CITRATE CELLULOSE NANOCRYSTALS Prapaporn Nakarat 1, * and Pattara Thiraphibundet 2 1 Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Nanotec-CU Center of Excellence on Food and Agriculture, Department of Chemistry, Faculty of Science, Chulalongkorn University and, Bangkok 10330, Thailand * aummy.6087@gmail.com Abstract: Citrate-modified bacterial cellulose nanocrystals (citrate CNCs) were prepared by a green one-pot dual acid (citric and hydrochloric acids) method. The size and degree of substitution of citrate CNCs are in a range of nm and 0.15, respectively. In this work, corn starch film reinforced with citrate CNCs (0-20 wt%) was prepared by the casting method. The images from field emission scanning electron microscopy (FE-SEM) showed a good dispersion of citrate CNCs within the starch matrix. The addition of citrate CNCs increased the decomposed temperature implying the thermal stability improvement of starch film. The mechanical property study revealed the increasing of tensile strength and Young s modulus by increasing the citrate CNCs amount. Therefore, citrate CNCs played an important role in improving the mechanical and thermal properties of the corn starch film. 253 The 44 th Congress on Science and Technology of Thailand (STT 44)

260 D_003_PF: PREPARATION OF DNA-DENDRIMER MOLECULAR HYBRID AS CARRIER FOR DRUG DELIVERY SYSTEM Paphada Watcharapo, Boonchoy Soontornworajit * Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani 12120, Thailand * sbooncho@tu.ac.th Abstract: Medical technology can facilitate the well-being of people, however, there are challenges in improving those technology. The purpose of this research was to prepare DNA-dendrimer molecular hybrid via amide bond formation using DCC/NHS as coupling agent. The DCC/NHS system is commonly used for modification of biomolecules. Dendrimer used in this study was a generation 5 of poly(amidoamine) (PAMAM). The scopes of this work covered effect of dendrimer to DNA molar ratios and ph conditions on coupling efficiency. The capability as a drug carrier was also investigated using Nile Red dye as a model. The coupling reaction was confirmed by gel electrophoresis method, which was capable of separating DNA and the molecular hybrid based on their sizes and charges. The results of this study showed that the conjugation between dendrimer and DNA using DCC/NHS as coupling agent was successful. The reaction yielded high amount of product. Moreover the reaction was also favorable in acidic condition. The release study indicated the abrupt rate of Nile Red release, thus this molecular hybrid system showed promises as drug carriers. 254 Science and Technology in the Disruptive Era

261 D_004_PF: TEMPO-OXIDIZED BACTERIAL CELLULOSE FOR REINFORCING ALGINATE-BASED FILMS Panida Watchanung 1 * and Pattara Thiraphibundet 2, 1 Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand 2 Nanotec-CU Center of Excellence on Food and Agriculture, Department of Chemistry, Faculty of Science, Chulalongkorn University and, Bangkok 10330, Thailand * panda_tookta@hotmail.com Abstract: TEMPO-oxidized bacterial cellulose (TOBC)-reinforced sodium alginate-based biodegradable films were prepared by solvent casting. Bacterial cellulose (BC) were oxidized by a 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated system. Some primary hydroxyl groups of BC structure were changed to be carboxylate groups to afford TOBC which has more effective distribution in aqueous suspension than BC. The fourier-transform infrared spectroscopy (FTIR) peak at 1605 cm 1 is attributed to carboxylate groups. Carboxylate contents could be determined as 0.15mmol/g by conductivity titration. The TOBC/alginate films were studied by varying the TOBC percentages (0-75wt% of alginate weight). Scanning electron microscopy images suggested uniform distribution of TOBC in the alginate film. The highest tensile strength of the TOBC/alginate film is 27.3 MPa which is 75% loading of TOBC in alginate films. The thermal properties of alginate films were not significantly different when the amount of TOBC was increased. 255 The 44 th Congress on Science and Technology of Thailand (STT 44)

262 D_005_PF: ACETALIZATION OF GLYCEROL USING SULFONIC ACID FUNCTIONALIZED ALUMINOSILICATE CATALYSTS UNDER SOLVENT-FREE CONDITION Ratikorn Vongkhae 1, Duangkamon Jiraroj 2, and Duangamol Nuntasri Tungasmita 2 * 1 Program in Petrochemistry and Polymer Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Center of Excellence in Catalysis for Bioenergy and Renewable Chemicals (CBRC), Chulalongkorn University, Bangkok 10330, Thailand * duangamol.n@chula.ac.th Abstract: Acetalization of glycerol with acetone was evaluated using sulfonic acid functionalized aluminosilicate catalysts under solvent-free condition to produce solketal as a final product. Several types of microporous and mesoporous supported catalysts were used in the reaction as zeolite beta and MCM-41, respectively. The propyl sulfonic functionalization on the porous materials was successfully synthesized by the grafting method with 3-mercaptopropyltrimethoxysilane group. All prepared catalysts were characterized the physical properties by powder X-ray diffraction (XRD), N 2 adsorption-desorption and acid-base titration. The effects of catalyst type, reaction temperature, reaction time and catalyst amount were studied. Comparison with catalyst types, H-beta-Pr-SO 3H showed the highest of solketal selectivity (95%) was analyzed by gas chromatography techniques. Moreover, the acetalization of glycerol with other ketone or aldehyde to produce five-membered ring and six-membered ring products such as propanal, acetophenone and benzaldehyde were also investigated over sulfonated catalysts. 256 Science and Technology in the Disruptive Era

263 D_006_PF: EFFECT OF MIXING TIME ON THE MECHNICAL PROPERTIES OF BIO-BASED FLEXIBLE POLYURETHANE FOAM Ruedee Jaratrotkamjorn, 1 Varaporn Tanrattanakul 1,2,* 1 Sino-Thai International Rubber College, Prince of Songkla University, Had Yai, Songkhla, 90110, Thailand 2 Faculty of Science, Prince of Songkla University, Had Yai, Songkhla, 90110, Thailand * varaporn.t@psu.ac.th Abstract: Bio-based flexible polyurethane foam (PUF) was synthesized with palm oil-based polyol (POP) and toluene-2,4-diisosyanate (TDI) by the one-shot method with the NCO/OH ratio of 1/1. POP containing hydroxyl value of 158 mg KOH/g was synthesized by the epoxidation and oxirane ring-opening reaction. The chemical structure of POP was determined by 1 H-NMR and FTIR techniques. A commercialized palm oil-based polyol (POLYGREEN, PG) was used for comparison with POP. PUFs were synthesized at different mixing times (40s, 50s and 60s). The effect of mixing times on the physical and mechanical properties of PUFs was determined. The mechanical properties testing included the compressive strength, compression set and hardness. PUF synthesized from POP showed higher density and mechanical properties than that from PG. 257 The 44 th Congress on Science and Technology of Thailand (STT 44)

264 D_007_PA: GREEN SENTHESIS OF SILVER NANOPARTICLES USING Cassia alata LEAF EXTRACT AND THEIR CATALYTIC ACTION IN REDUCTION OF DYE Abdulnaser Hajisamoh, Alesa Tohbaru, Kumari Dahama and Ashman Adair Chemistry Program, Faculty of Science Technology and Agriculture, Yala Rajabhat University, Yala, Thailand, Abstract: Green synthesis methods are regarded as safer alternative to the chemical, physical and microbial methods due to their environmental friendly nature, cost effectiveness and easy performance. In this study, a facile and eco-friendly procedure for the synthesis of silver nanoparticles (AgNPs) has been developed using leaf extract of Cassia alata, an abundantly available medicinal plant in the southernmost area of Thailand. Initially, leaf aqueous extract was fractionated by increasing order of polarity solvents using n-hexane, chloroform and ethyl acetate before being reacted with 1 mm AgNO 3. The color change and UV-visible absorbance Surface Plasmon Resonance (SPR) strong and wide band at 430 nm has initially confirmed the synthesis of AgNPs. The synthesized AgNPs were further characterized by Fourier Transform Infrared (FTIR), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), X-ray Diffraction (XRD) and Energy Dispersive X-ray Spectroscopy (EDX). The average size of synthesized AgNPs were in the range of nm. The catalytic effectiveness of synthesized AgNPs from Cassia alata leaf extract was investigated in catalytic degradation of dye (methylene blue). The results showed promising findings as good catalytic properties of AgNPs in reduction of this organic dye. 258 Science and Technology in the Disruptive Era

265 D_008_PA: FORMATION OF GELATIN PARTICLES VIA SCHIFF BASE REACTION IN EMULSION SYSTEM Paphitchaya Khomweerawong, 1 Boonchoy Soontornworajit 2, * sbooncho@tu.ac.th: Abstract: In this work, the gelatin particles were fabricated by using emulsification technique. Two types of surfactants were used; Sodium dodecyl sulfonate (SDS), and tween 20. Gelatin was firstly dissolved in water containing either one of those surfactant at different concentrations. Then the solution was transferred into a large volume of ethanol to form phase separation and glutaraldehyde was added to initiate a crosslink reaction. The formation of particles was facilitated by the reaction between aldehyde and amine group available in the two molecules. Ninhydrin assay demonstrated reduction of amino group due to the crosslink reaction, and FTIR spectra showed a characteristic peak of N=C- bond indicating the success of Schiff base reaction. The particles were further characterized by dynamic light scattering (DLS) and scanning electron microscope SEM. The DLS data showed that size of the particles were approximately 100 nm, and the distribution was narrow. SEM images demonstrated that the particles had a spherical shape and their sizes were consistent with the light scattering data. Moreover, surface charge of the particles was approximately -30 mv which allowed the particles to be stable in their suspension form. Therefore SDS and tween20 could facilitate the formation of gelatin particles. 259 The 44 th Congress on Science and Technology of Thailand (STT 44)

266 D_009_OF: PREPARATION OF NOVEL HIERARCHICALLY MAGNETIC POROUS CARBON MONOLITHS BY SURFACE SELF-ASSEMBLY COATING ON SUGARCANE BAGASSE SCAFFOLD. Ratchadaporn Kueasook, Laemthong Chuenchom* Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand * Abstract: The hierarchically magnetic porous carbon material (HPCM) with macro- and mesoporosity was successfully prepared by surface self-assembly coating of environmentally friendly phloroglucinol/glyoxylic acid precursors on bagasse surface. Triblock copolymer F127 was used as a mesopore-directing template and Fe 3+ ions from ferric chloride as a magnetic particle source. The preparation method was tuned to successfully coat the sugarcane bagasse scaffold with the precursor. After pyrolysis at 800 C, the monolith HPCM was obtained. To compare, the preparation of the sample without the templating techniques was performed by solely carbonizing the raw sugarcane bagasse with Fe 3+ ions under the same conditions (BGM). The obtained materials were characterized the physicochemical properties using various techniques. The surface chemical properties of both adsorbents are nearly the same. According to N 2 adsorption-desorption technique, HPCM demonstrates higher mesoporosity than BGM which lead to significant higher maximum adsorption capacity of methylene blue (MB) (76.40 mg/g) than BGM (5.18 mg/g) indicated that the existence of mesopore could enhance the adsorption property. Additionally, the HPCM monoliths have excellent floating ability over water surface due to its low density. The materials also have strong mechanical stability attributable to coated carbon procusor. After adsorption, The HPCM could also be facilely attracted from aqueous solution by an external magnet. The results indicated that HPCM is a promising adsorbent candidate for the waste water treatment. 260 Science and Technology in the Disruptive Era

267 D_010_OF: SYNTHESIS OF CdTe-MERCAPTOSUCCINIC ACID AND IN VITRO STUDY OF ITS CYTOTOXICITY USING MTT AND LDH ASSAYS Sonam Wangchuk, 1 Obnithi Noppha, 2 Yotsakorn Tantiapibalkun, 1 Kanlaya Prapainop. 1,2, * 1 Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand 2 School of Materials Science and Innovation, Faculty of Science, Mahidol University, Bangkok, Thailand * Kanlaya.pra@mahidol.edu Abstract: Quantum dots have unique fluorescent properties which can be applied widely in cellular imaging. However, there are still some concerns of cytotoxicity to the cells which could be varied from different synthesis method and surface coatings. Therefore, it is essential to evaluate the toxicity before applying them to the biological study. Here, we synthesized aqueous CdTe-mercaptosuccinic acid (CdTe-MSA) quantum dots and evaluated its toxicity by two different assays, lactate dehydrogenase (LDH) and MTT assays. The CdTe-MSA (0 nm-400 nm) was treated with two different cell lines: HEK 293T and MCF-7 cells for 24 hours. The results showed different sensitivity in detecting the early cytotoxic events comparing between two assays. The MTT assay was found to be more sensitive in detection of early cytotoxic events than the LDH assay in both two cell lines. In addition, HEK 293T cells were found to more sensitive to CdTe-MSA than MCF-7 cells. The results from this study showed that the cytotoxicity of the quantum dots should be carefully evaluated since the different methods and cell types could have effect on the reported toxicity level of the quantum dots. 261 The 44 th Congress on Science and Technology of Thailand (STT 44)

268 D_011_PF: INFLUENCES OF Ca/Si RATIO ON PHASES AND PROPERTIES OF HYDRATED LIME AND SILICA SAND MIXTURES UNDER HYDROTHERMAL CONDITION Aunsaya Eksatit, Karn Serivalsatit* Department of Materials Science, Faculty of Science, Chulalongkorn University, Thailand * Abstract: Calcium silicate hydrate phases are an important component in building materials. They are primarily responsible for the strength in cement-based materials. They have occurred by hydration reaction between hydrated lime and sand under hydrothermal condition. Calcium silicate hydrate phases can be formed in various compositions depending on Ca/Si ratio, which strongly affect properties of the building materials. The purpose of this work is to study phases and properties of hydrated lime and sand mixtures with Ca/Si ratios ranging from 0.2 to 1.0 under hydrothermal treatment at 180 C for 20 hours. Phases and morphology of the obtained specimens were investigated by XRD, FTIR, and SEM. Modulus of rupture of the specimens was measured by three-point bending method. The results showed that XRD pattern of the specimen with Ca/Si ratio of 0.2 indicated the presence of calcium silicate hydrate gel and unreacted quartz. As increasing Ca/Si ratio, the amount of calcium silicate gel increased. Tobermerite was observed in the specimens with Ca/Si over 0.6. Modulus of rupture of the specimens tended to increase as Ca/Si increased and reached up to MPa for the specimen with Ca/Si ratio of 1.0. Moreover, changing of Ca/Si ratio also affected morphology of calcium silicate hydrate phases. Morphology of the calcium silicate hydrate varied from plate-like shape to whisker as Ca/Si ratio increased. 262 Science and Technology in the Disruptive Era

269 D_012_OF: PREPARATION OF HYDROXYAPATITE AND PLATINUM-HYDROXYAPATITE FIBERS BY ELECTROSPINNING TECHNIQUE Titapa Tanawansombat, 1,2 Dujreutai Pongkao Kashima 1,2 Rojana Pornprasertsuk, 1,2,3, * 1 Department of Materials Science, Faculty of Science, Chulalongkorn University, Thailand 2 Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University, Thailand 3 Research Unit of Advanced Materials for Energy Storage, Faculty of Science, Chulalongkorn University, Thailand * rojana.p@chula.ac.th Abstract: Hydroxyapatite (HA) and platinum-hydroxyapatite (Pt-HA) fibers were prepared by electrospinning technique in this research work. HA powder was synthesized by precipitation method and mixed with polyvinylpyrrolidone (PVP) solution in ethanol for the HA fiber precursor. The obtained fibers were then calcined at 500 C for 6 h to decompose PVP and form HA fibers. The microstructure and phase analysis of the obtained fibers shows that the non-continuous fibers with only HA phase was obtained at the following preparation parameters: 200 mg/ml of HA, the feeding rate of 3.0 ml/h and the input voltage of 12 kv. For the Pt-HA fibers preparation, hydrogen hexachloroplatinate (IV) hydrate (H 2PtCl 6 6H 2O) at the Pt:HA weight ratio of 1:20 was added in the HA fiber precursor. The higher molecular weight of PVP was required to attain Pt-HA/PVP fibers. However, electrospinning and precursor preparation parameters must be further optimized to obtain the more uniform and continuous HA and Pt-HA fibers after calcination. 263 The 44 th Congress on Science and Technology of Thailand (STT 44)

270 D_013_PA: THE EFFECT OF CALCINATION TIME AND CALCINATION TEMPERATURE ON ENHANCING HYDROPHOBICITY OF MESOPOROUS SILICA NANOPARTICLES (MSNs) Tidapa Rattanaumpa, and Sittipong Amnuaypanich 2, * 1 Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand 2 Materials Chemistry Research Center, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand * asitti@kku.ac.th Abstract: Hydrophobic mesoporous silica nanoparticles (H-MSNs) were prepared by calcining MSNs at high temperature (700, 800 and 900 C) under inert gas condition. This study focuses on effects of calcination time and calcination temperature on a loss of surface silanol on MSNs. The results show that the amount of silanol gradually decreased with increasing temperature, because silanol group was converted to siloxane bond via dehydroxylation. The TGA results of MSNs show that the second weight loss indicated loss of surface silanol groups at temperature higher than 400 C. After calcination, all particle has a spherical shape with pore diameter in the range of 2-4 nm and no significant change of the particle morphology after calcination at C. When calcined at 900 C for 1 and 2 h, MSNs were transformed to core-shell particles with dense shell and porous core structure. After 3h at 900 C, MSNs were transformed to dense silica nanosphere. From FT-IR, a decrease in the peak intensity at 962 cm -1 band indicates the removal of Si-OH. XPS analysis revealed that a change of surface hydroxide and oxide species due to thermal treatments. For CO 2 absorption, most surface active sites were lost by calcination, leading to the loss of adsorption capacity for CO Science and Technology in the Disruptive Era

271 D_014_PF: PROPERTIES AND FRACTURE BEHAVIOR OF BAMBOO FIBER REINFORCED POLY(BUTYLENE SUCCINATE) AND POLYPROPYLENE COMPOSITES Supaphorn Thumsorn,* Worrathon Tilokkarn, Jessada Wong-On Department of Industrial Engineering, Faculty of Engineering, Pathumwan Institute of Technology, Bangkok 10330, Thailand * Abstract: Bamboo fiber (Bambusa blumeana) as a renewable reinforcing fiber was compounded with poly(butylene succinate) (PBS) in twin screw extruder at bamboo fiber contents of 0-20 wt% and fabricated to dumbbell by compression molding. The effect of bamboo fiber contents on morphology, mechanical performance, fracture behavior and thermal properties of PBS/bamboo fiber composites was investigated and compared with a conventional bamboo fiber reinforced polypropylene (PP) composites. Tensile strength of the PBS and PP composites slightly deceased with adding bamboo fiber. However, tensile modulus and impact strength of the composites increased when increasing bamboo fiber contents. The incorporation of bamboo fiber slightly increased crystallization temperature of the composites. It can be noted that bamboo fiber promoted crystallization and crystallinity of PBS and PP in the composites. Hence, high crystallinity of the composites would be better in tensile modulus and impact load transferring than neat polymer. SEM photographs showed good adhesion between bamboo fiber and PBS matrix while some fiber pulled out from PP matrix, which resulted in the declination of impact strength of the PP composites. 265 The 44 th Congress on Science and Technology of Thailand (STT 44)

272 D_015_OA: DEVELOPMENT OF ELECTROACTIVE POLYMER ACTUATORS EXPLOITING LOW COST PRECISE OPTOELECTRONIC MIRROR TECHNOLOGY Kritsadi Thetpraphi 1, Pierre-Jean Cottinet 1, Minh Quyen Le 1, Gil Moretto 2, Jeff Kuhn 3, Lionel Petit 1, Jean-Fabien Capsal 1, * 1 Univ Lyon, INSA-Lyon, LGEF, EA682, F-69621, Villeurbanne, France 2 Centre de Recherche Astrophysique de Lyon (CRAL), Saint-Genis-Laval, France 3 University of Hawaii s Institute for Astronomy, 34 Ohia Ku St, Pukalani, Maui, HI, USA * jean-fabien.capsal@insa-lyon.fr Abstract: The enhancement in the electromechanical coupling of optimized electroactive polymer (EAP) using as an actuator or sensor device is now still required to overcome the particularly high potential application like astronomy optical communication. In order to satisfy extremely smooth surface and deformable mirror replacing the classical polished rigid glass, terpolymer (P[VDF-TrFE-CFE/CTFE]) used as EAP will be integrated into the hybrid system (fig. 1). In previous works, the modified terpolymer doped with a plasticizer, e.g. Diisononyl phthalate (DINP) presented the large strain response and powerful mechanical energy density under low applied electric field. Continuing the scientific discovery, we proposed the idea of multilayer actuator breakthrough the impressive actuation performance by the increase of total axial strain from 0.4 % % with the sample 1 to 8 layers respectively under the low related electric field. The trial proof of mirror deformation concept has been demonstrated in this work. The final goal is to build up the extensive prototype of the actuator-sensor system allowing effective maintenance of the desired mirror shape. In addition, the modification we proposed here is cheap, industrially used and could potentially break a technological lock as the performance recorded at low electric field being greater than any conventional electroactive polymer. Figure 1. Schematic of hybrid mirror shape control system using in high-bandwidth optoelectronic communication system 266 Science and Technology in the Disruptive Era

273 D_016_PF: EFFECT OF ACID TREATMENT ON PROPERTIES OF NATURAL RUBBER/EPOXIDIZED NATURAL RUBBER/HALLOYSITE NANOTUBES COMPOSITES Kamaruddin Waesateh 1, Abdulhakim Masa 2, Nabil Hayeemasae 1 * 1 Department of Rubber Technology and Polymer Science, Faculty of Science and Technology, Prince of Songkla University, Pattani campus, Pattani Sino-Thai International Rubber College, Prince of Songkla University, Hat Yai campus, Songkhla, * nabil.h@psu.ac.th Abstract: Halloysite nanotubes (HNT) filled natural rubber (NR) filled was prepared in the presence of epoxidized natural rubber as compatibilizer.the raw HNT was treated by acid prior to compound with rubbers and other additives. Effects of treating time on the mechanical and morphological properties of the obtained composites were investigated. The surface area of acid-treated HNT was greater than that of untreated HNT and it increased over treating time. This has brought to an enhancement of stress at high strain especially when the strain over 500%. From the SEM images, the particle size of acid-treated HNT was seen to be finer and well-dispersed. This is attributed to the possibility of strong adhesion formation of HNT with NR and ENR. 267 The 44 th Congress on Science and Technology of Thailand (STT 44)

274 D_017_PA: FABRICATION OF NANOSTRUCTURE TiO 2 BY ANODIZATION METHOD Vanida lumpol, 1, Montri Aiempanakit 2, Cheewita Suwanchawalit 1* 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand 2 Department of Physics, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand * ; suwanchawalit_c@su.ac.th Abstract : We fabricated nanostructure TiO 2 via electrochemical anodization method. Titanium films were deposited on ITO substrates by using as Ti source. Titanium thin films immergred in water, ammonium fluoride (NH 4F) and ethylene glycol electrolytes to form nanostructure TiO 2 film. The effect of power vottage and concentration of NH 4F were investigated in current work.the nanostructure TiO 2 films were characterized by scanning electron microscope (SEM) and X-Ray Diffractometry (XRD) techniques. XRD results of the as-prepared TiO 2 films were amorphons phase. The SEM images of nanostructure TiO 2 films at 20V, 30V, 40V, 50V and 60V appeared nanoporous structure with inner average pore diameter about nm, nm, nm, nm and nm, respectively. Both fabrication parameter affected the morphology of nanostructure TiO 2 films. Nanostructured TiO 2 films could be potential use as a photocatalyst or photoanode of solar cell application. 268 Science and Technology in the Disruptive Era

275 D_018_PA: CURCUMIN-MODIFIED TITANIUM DIOXIDE NANOTUBES AND THEIR PHOTOCATALYTIC PERFORMANCE Montri Aiempanakit, 2 Thanatchaporn Tabtimsri, 1 Cheewita Suwanchawalit 1, * 1 Department of Physics, Faculty of Science, Silpakorn University, Sanam Chandra Palace Campus, Nakornpathom, Thailand Department of Chemistry, Faculty of Science, Silpakorn University, Sanam Chandra Palace Campus, Nakhon Pathom, Thailand * suwanchawalit_c@su.ac.th Abstract: Curcumin-modified TiO 2 nanotubes (Cur-TiO 2) were prepared by modified impregnation- electrochemical anodization method. The effect of curcumin loading on the surface of TiO 2 was studied. The physical properties of the curcumin-modified TiO 2 nanotubes were studied by several techniques such as X-ray powder diffraction (XRD), scanning electron microscopy (SEM), Energy Dispersive X-ray spectroscopy (EDS), Fourier-transformed infrared spectroscopy (FT-IR), and UV-Vis diffused reflectance spectroscopy (DRS). XRD patterns showed that TiO 2 sample and Cur-TiO 2 samples were anatase phase. SEM results revealed that the TiO 2 sample and Cur-TiO 2 samples had nanotube-like structures. The FT-IR spectra showed the characteristic bands of the TiO 2 and hydroxyl groups on the surface of the TiO 2 and the characteristic band of curcumin on the surface of Cur-TiO 2 samples. The EDS results confirmed the existence of Ti, O, C as the main elements of Cur-TiO 2 samples. The DRS spectra of Cur-TiO 2 samples extended into the visible region. The photocatalytic properties of Cur-TiO 2 samples were evaluated from the degradation of methylene blue under visible light irradiation. The Cur-TiO 2 samples exhibited higher photocatalytic performance than the TiO 2 sample under visible light irradiation. 269 The 44 th Congress on Science and Technology of Thailand (STT 44)

276 D_019_PA: NATURAL EXTRACT-BASED FLAME RETARDANT COTTON FABRIC Kittibandid Soisuwan 1, Warut Thammawichai 2 and Pattanawit Swanglap 1 * 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand 2 Navaminda Kasatriyadhiraj Royal Thai Air Force Academy, Bangkok, 10220, Thailand * yorke139@gmail.com Abstract: Cotton is one of the most widely used fabrics in clothing and textile because of its unique properties such as softness and comfortableness. However, its easy ignition and rapid flammability can lead to conflagration in the household. Therefore, improving flame retardant properties of cotton fabrics is desirable. Herein, the cotton fabric surface was modified by coating of a phytic acid-cpts sol-gel compound to increase the flame retardant of the fabric. Phytic acid is a natural flame retardant substance which can be extracted from natural grains. After modifying the cotton fabric with the flame-retardant compound, the modified cotton fabric was tested by burning in the flame of alcohol burner. It appeared that the time to ignition of the modified cotton fabric is slightly longer than the pristine cotton. The charring rate of the modified cotton fabric is 1.5 times slower than the pristine cotton fabric (12.14 mm/s compares to 8.16 mm/s). The burning test and flexibility test revealed that 4.5%PA-CPTS was the best condition of phytic acid-cpts sol-gel compound for coting of cotton fabric. The mechanism of flame retardant can be attributed to generation of char layer and reducing of flammable radical gasses. Furthermore, characterization by attenuated total reflectance infrared spectroscopy (ATR-IR) revealed that the cellulose characteristic peak (O-H stretching at 3334 cm -1 ) of the modified cotton fabrics is slightly weaker than the cellulose characteristic peak of the pristine cotton fabric, which implied that the coating of the flame retardant compound occurred through covalent bonding. 270 Science and Technology in the Disruptive Era

277 D_020_PF: PREPARATION OF NANOFIBER CELLULOSE/POLY(VINYL ALCOHOL) FILMS AND THEIR PROPERTIES Butsarin Thaweekoon, 1 Sutinee Girdthep, 1 Cheewita Suwanchawalit, 1,* 1 Department of Chemistry, Faculty of Science, Silpakorn University, Sanam Chandra Palace Campus, Nakornpathom, Thailand * suwanchawalit_c@su.ac.th Abstract: The objective of this work was to prepared nanocomposites consist of nanofiber cellulose (NCFs) isolated from rice straw and polyvinyl alcohol (PVA). The nanofiber cellulose was prepared by chemical and mechanical treatment. Nanocomposite films were prepared by solution casting. The morphology and size of nanofiber cellulose were study by using Scanning electron microscopy (SEM) techniques. The SEM results showed uniform rod structure of fibers with the width distribution around on the range of nm and serveral 4 µm in the length. The structure and functional group of NCFs and nanocomposites were characterized using XRD and FTIR technique. Moreover, the obtained cellulose nanofibers have great aspect ratio and high potential in reinforcement. The mechanical properties results found that PVA/1300 rpm nanocomposite has a great strength in this study. 271 The 44 th Congress on Science and Technology of Thailand (STT 44)

278 D_021_PF: GEMOLOGICAL CHARACTERISTICS AND CHEMICAL COMPOSITIONS OF COLOR- CHANGE GLASS BEING MARKETED AS SYNTHETIC ZULTANITE Tanyaporn Tiaotrakoon, 1 Kanyarat Kwansirikul 1, * 1 Department of Geological Sciences, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand * kanyarat.k@cmu.ac.th Abstract: Twenty-nine color-change materials being marketed as synthetic zultanite were studied gemological characteristics and chemical compositions using standard gemological methods and advanced techniques in gem analyses. These color-change materials were cut in round brilliant style. They showed bluish green in fluorescent light and yellowish brown in incandescent light. Specific gravity of all samples ranged from 3.17 to The materials showed one refractive index varying from to This indicated that the studied samples were isotropic material. Under magnification, some of the samples exhibited flow-line internal feature. Absorption spectra and chemical data revealed major elements of SiO 2, K 2O, PbO, Nd 2O 3 and Pr 2O 3 while minor and trace elements were Gd 2O 3, Ce 2O 3, ZnO and Al 2O 3. The most significant trace elements were Nd and Pr that were the chromophores for color-change causing in the studied materials. The properties mentioned above, along with the chemical data, indicated that the studied materials were not synthetic zultanite but rather color changing man-made glass. 272 Science and Technology in the Disruptive Era

279 D_022_PF: UTILIZATION OF AGRICULTURAL WASTE AS ABSORBENT FOR THE REMOVAL OF PHENOL RED AND BROMOCRESOL GREEN FROM AQUEOUS SOLUTION Pimchanok Phatho, 1,2 Sutthida Boomsamran, 3,4 Khamphe Phongthong, 3,4 Thitipone Suwunwong 1,2 * 1 School of Science, Mae Fah Luang University, Tasud, Muang, Chiang Rai, 57100, Thailand 2 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Tasud, Muang, Chiang Rai, 57100, Thailand 3 Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla 90112, Thailand 4 Center of Excellence on Hazardous Substance Management (HSM), Bangkok 10330, Thailand * thitipone.suw@mfu.ac.th Abstract: Biochar from agricultural waste was prepared by gasification process for the application of the resulting biochar for water treatments. In this study, we investigated gasification biochar materials originating from corncob with BET technique. The result showed that adsorption average pore diameter of biochar is Å and the pore volume of biochar is observed to be 0.26 cm 3 /g. The obtained biochar was used as an absorbent for the removal of triphenylmethane dyes, phenol red and bromocresol green, in aqueous solution, The absorption of biochar on phenol red and bromocresol green is presented as the amount of dye adsorbed, Qe (mg/g). It was found that the absorption amounts of these triphenylmethane dyes on the biochar surface are increased with the increasing times. 273 The 44 th Congress on Science and Technology of Thailand (STT 44)

280 D_023_PF: EFFECTS OF VULCANIZATION SYSTEM ON PROPERTIES OF SILICA REINFORCE NATURAL RUBBER COMPOSITES PREPARED FROM FRESH LATEX Nustsaba Kronburee, Pimsiree Suwanna* Department of Materials Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand * Abstract: This research studies processing of silica/natural rubber masterbatch and the properties of silica reinforced natural rubber composites prepared using three different vulcanization systems. The silica/natural rubber masterbatch was prepared by a wet method, where silica dispersion was added into natural rubber latex and then coagulated by acid to form coagulum. The masterbatch was compounded with other ingredients in different sulfur/accelerator ratios based on a conventional vulcanization system (CV), a semi-efficient vulcanization system (Semi EV) and an efficient vulcanization system (EV). Scanning electron microscopy (SEM) was used to examine the dispersion and distribution of silica in the natural rubber matrix. To investigate mechanical and thermal properties of the rubber compounds, we measured their tensile properties, hardness, tear and abrasion resistance, compression set and heat build-up. The results show that the mechanical properties of a CV system are generally better than those of Semi EV and EV systems. However, an EV system yields higher thermal resistance than CV and Semi EV systems. 274 Science and Technology in the Disruptive Era

281 D_024_OA: ETHANOL OXIDATION REACTION ON NICKEL-BASED CATALYSTS Wilaiwan Supap 1, Thaneeya Hawiset 2, Somwan Chumphongphan 1,3, Prachak Inkaew 1,4 * 1 School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand 2 School of Medicine, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand 3 Materials for Energy and Environment Research Group, School of Science, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand 4 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand * prachak.ink@mfu.ac.th Abstract: Direct ethanol fuel cells (DEFCs) have a potential to be used as power sources for electric vehicles and portable electronic devices. In general, platinum (Pt) is the most common catalyst used in ethanol oxidation reaction (EOR), the reaction on anode side of the DEFCs. However, since Pt is very expensive, other metals have been investigated and developed to be used instead of Pt. In this work, Ni-based catalysts were prepared via electrodeposition and sonochemical method. Various bimetallic catalysts of Ni and other metals such as Pd, Bi, Ru, Co were prepared and characterized. The EOR catalytic activity and stability, along with surface compositions, surface structure, and morphologies of the prepared catalysts were performed. Cyclic voltammetry, chronoamperometry, X-ray defection, X-ray fluorescence and scanning electron microscope were used to characterize the catalysts. The electrocatalytic activity and stability of Ni-based catalysts were investigated toward ethanol oxidation in alkaline media. The results were compared to those of commercially available Pt catalyst. The addition of second metals affects electrocatalytic activity and stability of nickel-based catalysts. The prepared catalysts showed high catalytic activity and stability, and had a potential to be used in DEFCs. 275 The 44 th Congress on Science and Technology of Thailand (STT 44)

282 D_025_PF: REDUCED GRAPHENE OXIDE/COBALT SULFIDE HYBRID FILM FOR PHOTOCATALYTIC DEGRADATION OF DYE Anwaraporn Suramitr 1, *, Kannika Boonkue 1, Wannisa Arthan 1, Songwut Suramitr 2, 3 1 Faculty of Science at Sriracha, Kasetsart University, Sriracha Campus, Chonburi 20230, Thailand 2 Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand 3 Center for Advanced Studies in Nanotechnology for Chemical, Food, and Agricultural Industries, Kasetsart University, Bangkok 10900, Thailand * sfsciawn@src.ku.ac.th Abstract: Reduced graphene oxide/cobalt sulfide (rgo/cos) hybrid films coated on FTO glass were synthesized by a simple hydrothermal method for photocatalysis application. The hybrid films were characterized by using Scanning electron microscope (SEM), Energy Dispersive Spectroscopy (EDS) and X-ray diffraction (XRD). The effects of rgo concentration loading and H 2O 2 for the photocatalytic degradation of methylene blue (MB) in aqueous solution was investigated under visible light irradiation. Furthermore, the rate constant (k) for the photodegradation of the MB was determined under pseudo-first order conditions. The hybrid film of rgo 0.05/CoS shows the highest efficiency, which the photocatalytic degradation with H 2O 2 addition is 100% within 120 minutes with k as min Science and Technology in the Disruptive Era

283 D_026_PA: EVALUATION OF ANTIBACTERIAL AND ANTI-FREE RADICAL ACTIVITIES OF COTTON FABRIC STAINED WITH NATURAL DYE USING CHITOSAN AS MORDANT Sucharat Sanongkiet 1, * 1 Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, Thailand * sanongkiet_s@silpakorn.edu Abstract: Chitosan is a well-known biomolecule which composed of a natural polycationic linear polysaccharides derived from chitin. Chitosan have been used in many application such as biocompatibility, non-toxicity and low allergenicity. In this work, chitosan was used as a mordant compared with alum and carboxymethy cellulose (CMC) before dyeing with sappan wood. The color strength and physical properties were investigated by using spectroscopy, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR) respectively. Antibacterial properties were evaluated on Staphylococcus aureus and Escherichia coli. Moreover, antioxidant property was also determined by using 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay. The results demonstrate that chitosan improve the biological properties of the cotton fabric dying with sappan wood both antibacterial and anti-free radical properties compare with the other mordants. This knowledge will help to increase the value of cotton fabrics stained with natural dye. 277 The 44 th Congress on Science and Technology of Thailand (STT 44)

284 D_027_OA: CARBON ADSORBENTS FROM SUGARCANE BAGASSE PREPARED THROUGH HYDROTHERMAL CARBONIZATION FOR ADSORPTION OF METHYLENE BLUE: EFFECT OF HEAT TREATMENT ON ADSORPTION EFFICIENCY Preeyanit Buapeth 1, Decha Dechtrirat 2 and Laemthong Chuenchom 1, * 1 Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand 2 Department of Materials Science Faculty of Science, Kasetsart University, 50 Ngamwongwann Rd., Ladyao, Chatuchak, Bangkok * laemthong.c@psu.ac.th Abstract: A carbon hydrochar material (BG-230) has successfully been prepared by conversion of sugarcane bagasse through low temperature hydrothermal carbonization (HTC) at 230 C. BG-230 was subsequently subject to further thermal treatment at 400 C for 1 hour under air (BG-230/400). Both resulting materials were characterized using specific surface area analysis (BET), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis (C, H, N, O) and X-ray photoelectron spectroscopy (XPS). The characterization results show that BG-230 possesses a higher number of acidic-oxygen functionalities than BG-230/400, indicating that the thermal treatment at 400 C under air removed those functionalities. The adsorption properties were evaluated by the removal of methylene blue (MB) from aqueous solutions. The adsorption capacity of MB was found to be higher for BG-230 ( 100 mg g -1 ) than that for BG-230/400 ( 25 mg g -1 ). The adsorption results suggest that the oxygen functionality of as-prepared carbon materials is responsible for the adsorption mechanism. 278 Science and Technology in the Disruptive Era

285 SESSION E: ENERGY / ENVIRONMENTAL& EARTH SCIENCE 279 The 44 th Congress on Science and Technology of Thailand (STT 44)

286 E_001_PA: THE DEPOSITION OF DUST FALL ON ROADSIDE IN FRONT OF NARESUAN UNIVERSITY Punlop Tonapo, 1 Weerapat Surasaeng, 2 Nipitpon Kongjan 3, Pajaree Thongsanit 4, * 1-3 Graduated bachelor s degree of environment engineering, Department of civil engineering, faculty of engineering, Naresuan university, Muang, Phitsanulok, Thailand 4 Asisstant Professor, Department of civil engineering, faculty of engineering, Naresuan university, Muang, Phitsanulok, Thailand * pajareet@hotmail.com Abstract: This study aimed to determine amount of dust fall on the roadside in front of Naresuan University. The samples were collected along the main road for five stations. The dust fall sampling was conducted using dust fall jar container method. The dust fall sampling equipment was including of the water sampling cylindrical bottle with a diameter of about 13 cm, height 20 cm. The stand of water samples container was used 1.5 m long pipe with the basket for bottle. (figure1). The concentration of the dust was analyzed by weight measurement or gravimetric method. The samples were taken during October 2015 to February Each sample was collected in every thirty days therefore twenty five samples were taken. The obtained data were calculated and compared the amount of dust fall with Malaysia air quality standard value of 200 mg/m²/day. The results indicated that there were eight samples exceeding standard (32%).The highest level of dust fall was found in February 2016 by the entrance of Shell gas station with the level of mg/m²/day. This could be potentially from blowing dust from vehicles entering the area and adjusting soil level at side road which increasing the spread of dust in the surrounding areas. (A) (B) Figure 1. Dust fall jar container (A) Adapted from equipment of department of pollution control, Thailand (B) Figure 2. The levels of dust fall in five roadside stations in front of Naresuan University 280 Science and Technology in the Disruptive Era

287 E_002_OF: FOUNDATION THE WEIGHT SET FOR THE WATER QUALITY INDEX S FORMULA Bui Viet Hung Faculty of Environment, University of Science, Vietnam National University Ho Chi Minh City, Vietnam * bvhung@hcmus.edu.vn Abstract: The water quality assessment by the water quality index (WQI) follow the decision 879/QD-TCMT of Ministry of Natural Resources and Environment (MONRE) is applied very broad in most of the Vietnam s provinces. In the process of application, WQI has revealed many limitations as not reflecting the state of real water use and local socio-economic, environment, and be incorrected when the measured data used into the calculation has mutative high values. For the solution, the paper presents how to determine the weight set of quality sub-indexes in the WQI s formula. The foundation of determination is based on a fuzzy comprehensive evaluation theory combined with a weighting entropy procedure. With the observed data set on the rivers and canals system in Tien Giang province is an example to highlight the reasonability, logical and suitability of the surface water quality index s formula adjusted by the new weight set of the water quality sub-indexes. Keywords: water quality, water quality index, surface water quality assessment, fuzzy comprehensive evaluation, weight entropy procedure. 281 The 44 th Congress on Science and Technology of Thailand (STT 44)

288 E_003_OA: SEISMIC GEOMORPHOLOGY OF GIANT FORESETS FORMATION, TARANAKI BASIN, NEW ZEALAND Waris Nuamnim,* Piyaphong Chenrai Basin Analysis and Structural Evolution Special Task Force for Activating Research (BASE STAR), Department of Geology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * Abstract: The Taranaki Basin covers an estimated area of 330,000 along the western side of the North Island, New Zealand. There is currently the only producing petroliferous basin in New Zealand. The study focuses on the geomorphology and seismic reflection characteristics of the Plio-Pleistocene Giant Foresets Formation to understand how clinoforms develop, control factors of geometry and sediment distribution. The Giant Foresets Formation is characterized by rapid progradation of late-early Pliocene to Recent which constructed the modern continental shelf and slope on western side of the North Island of New Zealand. Seismic data of The Giant Foresets Formation shows development of clinoforms and reaches a thickness of 2200 m. The high thickness and rapid accumulation of The Giant Foresets Formation will have a significant impact on the petroleum systems of Taranaki Basin, particularly in the maturation of hydrocarbons and their migration. 3D seismic data are the main data that used in this study in order to understand geomorphology in subsurface including characteristics of channels. In this study area, small-scale channels with thin bed tuning cause a difficult in seismic detection and interpretation, thus seismic attributes used in this study for extracting more information from the seismic data will help to illustrate the geomorphological features of the area. The Giant Foresets Formation is considered to have minor reservoir potentials in terms of containing sandstone-dominated stratigraphic traps, but it can be used as an analog model for channelized sandstone in deeper part of seismic data with low. 282 Science and Technology in the Disruptive Era

289 E_004_OF: SEISMIC CHARACTERISTICS OF PALEO-POCKMARKS AT THE GREAT SOUTH BASIN, NEW ZEALAND Arunee Karaket, and Piyaphong Chenrai* Basin Analysis and Structural Evolution Special Task Force for Activating Research (BASE STAR), Department of Geology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * Abstract: Paleo-pockmarks and present-day pockmarks are founded in several varieties both onshore and offshore worldwide. These features can be used as an indicator of fluid flow process which expulses through an unconsolidated sediment within the basin. The Great South Basin, New Zealand is one of the basins that paleo-pockmarks are observed and associated with polygonal fault cover the southeast offshore at around 1.5 s (TWT) depth beneath the surface. Thus, this paper aims to identify the characteristics of paleo-pockmarks in the Great South Basin. The numerous paleo-pockmarks are identified and imaged by using 3D seismic data. Paleo-pockmarks in this area are observed on the fine grain sediment of middle Eocene top formation as known Laing formation. The fan shape distribution of all paleo-pockmarks is aligned from the southwest toward northeast direction with high density around 336 paleo-pockmarks per five square kilometers located in the central of the study area. Most paleo-pockmarks in this area have a similar shape but varies in size that is sub-round to round in shape and size ranging from 138 to 382 m in diameter and 15 to 45 ms (TWT) in depth. Progradational pattern of clastic sediment in the lower part of the paleo-pockmark formation is possibly to related to fluid pathway and mechanism of paleo-pockmarks in the Great South Basin. 283 The 44 th Congress on Science and Technology of Thailand (STT 44)

290 E_005_PF: CHARACTERISTICS AND POSSIBILITIES OF INCINERATION RESIDUES FROM BIOMASS-FIRED POWER PLANT IN ROAD CONSTRUCTION Sutthida Boonsamran, 1, * Kuaanan Techato, 1,2 Tanan Chub-uppakarn, 3 Khamphe Phoungthong 1,2 1 Environmental Assessment and Technology for Hazardous Waste Management Research Center, Faculty of Environmental Management, Prince of Songkla University, Songkhla 90112, Thailand 2 Center of Excellence on Hazardous Substance Management (HSM), Bangkok 10330, Thailand 3 Department of Civil Engineering, Faculty of Engineering, Prince of Songkla University, Songkhla 90112, Thailand * sutthida.bo@outlook.co.th Abstract: Physical-chemical characteristics and the leaching tests of fly- and bottom ash from biomass-fired power plant were investigated. The experimental methods were designed to analyze the technical suitability, chemical components and in addition morphological, mineralogical properties of using fly- and bottom ash. This work focused on the use of incineration residues as an aggregate substitute in sub-base. The results show that these residues may be successfully used as an alternative material in unbound road sub-base. According to Thai specifications for road construction. Based on the research in this study demonstrated significantly, that the evaluating of tests to assess the environmental and human risks of fly- and bottom ash before decisions for road construction. 284 Science and Technology in the Disruptive Era

291 E_006_PF: MATERIAL FLOW ANALYSIS FOR WASTE MANAGEMENT IN ONSHORE PETROLEUM EXPLORATION AND PRODUCTION INDUSTRY IN THAILAND Noulkamol Arpornpong, 1,2, * Gitsada Panumonwatee, 1 Ampira Charoensaeng, 2,3 Sutha Khaodhiar, 2,4 Chodchanok Attaphong 2,5 1 Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Thailand 2 Research Program of Industrial Waste Management Policies and Practices, Center of Excellence on Hazardous Substance Management (HSM), Thailand 3 The Petroleum and Petrochemical College, Chulalongkorn University, Thailand 4 Department of Environmental Engineering, Chulalongkorn University, Thailand 5 Faculty of Engineering, King Mongkut's Institute of Technology Ladkrabang, Thailand * noulkamola@nu.ac.th Abstract: Sustainable development is the main driving force for improving waste management in the petroleum production sector in Thailand. In this studied, the types and characteristics of waste, and existing waste management options from seven onshore petroleum concessionaires were identified. The data used in this study were gathered from 2015 annual waste management report by the Department of Mineral Fuels, Thailand. The results indicate that the waste disposals in 2015 were 18 types of non-hazardous waste, 13 types of HM waste, and 8 types of HA waste with generated from the petroleum drilling and production processes. Additionally, Material Flow Analysis (MFA) was applied to study the route and amount of disposal wastes following into their end-of-life (EOL) waste management. The results show that the major streams of the hazardous waste disposal were the produced water from petroleum production process (4,804,646 t/yr), the oil contaminated liquid (217,366 t/yr), the bottom-hole drilling cutting (34,048 t/yr), and the oil sludge (1,411 t/yr). Next, the comprehensive 3R methodology will be used to propose the alternative end-of-life (EOL) waste management options. In order to reduce environmental impacts and maximize waste utilization, the proposed EOL and existing treatments will be evaluated and compared though life cycle analysis. 285 The 44 th Congress on Science and Technology of Thailand (STT 44)

292 E_007_OF: ASSESSMENT OF COLIFORM BACTERIA CONTAMINATION IN DRINKING WATER SOURCES OF HILL TRIBE LIVING AREA IN CHIANG RAI Korakot Chansareewittaya, 1, * Sitang Kongkratoke, 1 Pannipha Dokmaingam, 1 Tawatchai Apidechkul 1,2 1 School of Health Science, Mae Fah Luang University, Chiang Rai, Thailand 2 Center of excellence for the hill tribe health research, Mae Fah Luang University, Chiang Rai, Thailand * korakot.cha@mfu.ac.th Abstract: Coliform bacteria are the commonly-used bacterial indicator of sanitary quality of water since it is not necessary to analyze drinking water for all pathogens. The presence of total coliform bacteria (TCB) and fecal coliform bacteria (FCB) are used to indicate the contamination of pathogenic organisms and organisms of fecal origin may be present in the water. Safe drinking water which free from any pathogenic bacteria is required for public health protection. Therefore, no coliform bacteria are allowed in drinking water. This study aims to measure and to assess the contamination of coliform bacteria in several drinking water sources in the living area of 6 hill tribes population in northern Thailand, Chiang Rai. TCB and FCB were measured by means of the multiple-tube fermentation technique and estimated TCB and FCB contamination as Most Probable Number (MPN) index/100 ml. Physical parameter (ph) was also determined by using ph meter. Forty-four samples of drinking water were taken from several drinking water sources. The results showed that the ph of the water samples ranged from 5 to 7.5, while the odor, color and turbidity of the water samples were natural. TCB and FCB were found in 84.1% of total water samples. TCB and FCB level was in the range of MPN index/100 ml < 3 to 1100, which exceeded the national drinking-water quality standard (Department of Health, Ministry of Public Health, Thailand, 2000) % and 8.11% of water samples were estimated to be very high ( 180) MPN of TCB and FCB, respectively. Since the water samples collection was taken during the wet (rainfall) season, then it was expected from these results that coliform bacteria contamination tended to associate with rainfall that moved downward coliform bacteria from nearby environment and led them spreading into the water sources. Therefore, year-round monitoring of coliform bacteria distribution was suggested for further study. Detection of contaminated coliform bacteria in examined water sources may contribute to health problems among hill tribe s populations and appropriated prior water treatment is required for consuming purposes and other uses. 286 Science and Technology in the Disruptive Era

293 E_008_OF: LONG CHAIN n-alkane DISTRIBUTIONS IN THE MANGROVE PLANTS Assuma Sainakum 1, Akkaneewut Chabangborn 1,*, Piyada Jittangprasert 2 and Penjai Sompongchaiyakul 3 1 Morphology of Earth Surface and Advanced Geohazards in Southeast Asia Research Unit (MESA), Department of Geology, Faculty of Science, Chulalongkorn University, Bangkok, THAILAND 2 Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, THAILAND 3 Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, THAILAND * akkaneewut@gmail.com Abstract: Although the biomarkers are recently interesting proxy for paleoenvironment studies, the database of n-alkane distributions in plant leaf waxes are very limited. We here investigated the n-alkane distributions of sixteen plant leaf samples from four locations, i.e. Thale Noi (TLN), Kung Krabaen Bay (KKB), Khun Samut Chin Temple (KSC) and Pom Phra Chulachomklao (PPC). The leaf waxes were extracted with a mixture of dichloromethane and methanol (9:1, v/v) using ultrasonic extractor. The different polar hydrocarbon fractions were subsequently separated by silica gel solid phase chromatography and %abundance of n-alkanes were analyzed by gas chromatography-mass spectrometry (GC/MS). The n-alkane distributions were further considered by the maximum carbon number (MCN), the average chain lengths (ACL) and the carbon preference index (CPI). The mangrove plants are characterized by long chain with odd number n-alkane and higher CPI values than those from other samples. C 24 is the most predominant carbon atom in the samples from back mangrove and freshwater environments. The CPI values obtained by back mangrove plants are generally about 1, while these values are mostly less than 1 in the plants from the freshwater environment. These can be explained by the plant modifications under the tolerant environments. 287 The 44 th Congress on Science and Technology of Thailand (STT 44)

294 E_009_OF: APPLICATION OF DIFFERENTIAL SAR INTERFEROMETRY FOR GROUND DISPLACEMENT MONITORING IN WESTERN MOUNTAIN RANGE BESIDE THE PHETCHABUN BASIN, CENTRAL THAILAND Nuttavit Kumvijairat 1 Pitsanupong Kanjanapayont 1,* 1 Basin Analysis and Structural Evolution Special Task Force for Activating Research (BASE STAR), Department of Geology, Faculty of Science, Chulalongkorn University, Bangkok THAILAND pitsanupong.k@hotmail.com Abstract: Ground displacement detection is the best way to know how surface deformation of interesting area. It can represent scale of displacement with millimetric precision. Therefore, Differential Synthetic Aperture Radar interferometry (DinSAR) was applied to measured ground displacement. DinSAR technique has been widely used for geological hazard investigating on subsidence, landslide, earthquake and volcanic activity. This study used two-pass DinSAR for measured ground displacement in western mountain range beside the Phetchabun Basin in central Thailand. The result of deformations was represented in meter, that show ground displacement to satellite line of sight. Maximum surface deformation and quantity of deformation area is 10 centimeter and 0 1 centimeter. The ground displacement has been formed latitude 101º3 0 E to east side. This area consisted of Late Triassic sedimentary rocks (shale, mudstone, siltstone, graywacke and argillaceous limestone) and high step slope, that make high displacement form mass movement in monsoon season. Although Phetchabun range have high displacement but Khao Kho district has low displacement. The displacement around urban area in Khao Kho district mainly caused by land cover change. 288 Science and Technology in the Disruptive Era

295 E_010_OF: OXIDATION STATES OF TRACE ELEMENTS IN SYNTHETIC CORUNDUM Ontima Yamchuti 1*, Waruntorn Kanitpanyacharoen 1, Chakraphan Sutthirat 1, Wantana Klysuban 2, Penphitcha Amonpattarakit 2 1 Department of Geology, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok Thailand Synchrotron Light Research Institute (Public Organization), 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand * y.ontima29@gmail.com Abstract: Corundum occurs in various colors due to impurities or trace elements in its structure. Sapphire and ruby are essentially the same mineral, corundum, but valued differently due to their red and blue varieties. Color is one of the critical factors used to determine the value of natural and synthetic corundum. Despite the abundance of research on impurities in natural corundum, little is known about trace elements in synthetic corundum. This project thus aims to quantify trace elements and identify their oxidation states in synthetic corundum. A total of 15 corundum samples in red, blue, and yellow, synthesized by melt growth process, were first investigated by X-ray diffraction (XRD) analysis to determine the composition. Electron probe micro-analyzer (EPMA) was used to identify the types of trace elements. Results confirm that all samples contain crystalline Al 2O 3 and various types of trace element, particularly Cr, Fe, and Ti. To further determine the oxidation states of trace elements, synchrotron X-ray absorption near edge structure spectrometry (XANES) was used to observe absorbing energy of each element. XANES results of red synthetic corundum show Cr 3+ as a major trace element (62%), which has pre-edge absorption energy at 6001 ev. In addition, Fe 2+ and Fe 3+ are dominant oxidation states of yellow synthetic corundum while Ti 3+ and Ti 4+ are dominant oxidation states of blue synthetic corundum. The average absorption energy of Fe and Ti is 4980 ev and 7113 ev, respectively. The presence of Fe 2+, Fe 3+, Cr 3+, Ti 3+, and Ti 4+ in synthetic corundums is consistent with the observation of natural corundums in previous studies. Other trace elements such as Mg 2+, which have been observed in yellow natural corundums, are not detected in this study. Our results suggest that red and blue synthetic corundums have similar types and oxidation states of trace elements as those of natural corundums. However, our yellow synthetic corundums have fewer varieties of trace elements and oxidation states than those are found in the natural origins. 289 The 44 th Congress on Science and Technology of Thailand (STT 44)

296 E_011_PF: BIODIESEL PRODUCTION FROM WASTE COOKING OIL USING CALCIUM OXIDE DERIVED FROM WASTE STARFISH AS HETEROGENEOUS CATALYST Apisit Prokaew 1, Supakorn Boonyuen *1, Siwaporn Meejoo Smith 2 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand 2 Department of Chemistry, Faculty of Science and Technology, Mahidol University, Nakorn Pathom, Thailand * chemistrytu@gmail.com Abstract: The waste starfish was sinthered and used as the hetero generous catalyst CaO. The produced CaO was utilized as a catalyst for biodiesel production from waste cooking oil. The waste starfish is a potentially source of calcium carbonate which potentially changes to calcium oxide in calcination temperatures 800 C. The synthesized catalysts were characterized by using Thermogravimetric analysis (TGA), X-ray diffraction (XRD), Scanning electron microscopy (SEM) analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Hammett indicator method. The TGA result shows the starfish completely to convert from CaCO 3 to CaO when used calcination temperature at 800 C and the XRD pattern of catalyst found the calcium peak that can confirm phase change of calcium oxide catalyst from waste starfish. Transesterification reaction was done in the presence of waste cooking oil, methanol and starfish catalyst at a temperature of 80 C. The result show, the waste starfish catalyst gives the biodiesel conversion higher than 90% when used methanol to oil ratio 5:1, reaction time 3 h catalyst concentration 9%wt. and catalyst can be reused more than 5 cycles with the high biodiesel conversion( >60%) 290 Science and Technology in the Disruptive Era

297 E_012_OF: INDOOR AIR QUALITY ASSESSMENT IN GOVERNMENT HOSPITAL IN NORTHERN THAILAND Wanwisa Kansa, Theetawat Sriwichaikaew, Patcharamporn Khamchana, Sitang Kongkratoke* Department of Occupational Health and Safety, School of Health Science, Mae Fah Luang University, 333 Moo1, Thasud, Muang, Chiang Rai, 57100, Thailand * Abstract: Indoor air quality in hospitals is very importance to hospitals staffs and patients. Poor ventilation may cause of airborne disease such as tuberculosis and legionellosis. This research aims to study about the ventilation efficacy in natural ventilation system which determine the efficacy by indoor air quality indicators which consist of CO 2 accumulated level, temperature, relative humidity, flow rate and exchange rate in 4 rooms in hospital building there are Outer Patient Department (OPD), Non-Communicable Disease (NCD), Female Inner Patient Department(IPD F) and Male Inner Patient Department(IPD M). The monitoring result was used to analyses and compared with ASHRAE standard and lead to conclusion From the compared the data with standard found the ventilation system efficacy in 4 rooms of government hospital shown that air change rate, number of people in measurement date and relative humidity non-conform with the standard in every room and CO 2 concentrations in some location in NCD over 1,000 ppm. Those of data show that the ventilation system in this hospital is inefficiency because the ventilation system cannot ventilated to provide the air enough for each area and other causes of ventilation system inefficacy was the number of room s user over the recommend. The result of this study was used to provide the suggestions to add more electric fan to improve air flow in hospital and considerate to administrative control to manage number of people in each room. 291 The 44 th Congress on Science and Technology of Thailand (STT 44)

298 SESSION F: PHYSICS / APPLIED PHYSICS 292 Science and Technology in the Disruptive Era

299 F_001_OA: EXPERIMENTAL-BASED PROBABILITY INTERPRETATION OF PHOTON-RELATED EVENTS IN DIRAC THREE-POLARIZER EXPERIMENTS Pawaphat Jaturaphagorn, 1 Unchittha Prasatsap, 1 Suwit Kiravittaya 1,* 1 Advanced Optical Technology (AOT) Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, Naresuan University, Phitsanulok 65000, Thailand. * suwitki@gmail.com Abstract: Light intensity signal obtained from the Dirac three-polarizer experiment that shows the weirdness of photon properties is experimentally measured. The setup consists of a green diode laser, three polarizers (P1, P2, P3), and a photodetector. They are aligned in-line as shown in Fig. 1(a). For the calibration experiment, the measurement is done with two polarizers (P1 and P2). In the three-polarizer experiment, the first and the last polarizers (P1 and P2) are set constant at perpendicular angle. So no light can transmit if the middle polarizer (P3) is not presented or if it is presented with the angle parallel to either the first or the last polarizer. When the polarization angle of the middle polarizer is varied, the obtained light signal follows the theoretical relation based on the classical optics and the transmitted light signal is maximized at the polarization angle of 45 relative to the first and the last polarizers. Realistic optical properties of the components such as transmission and reflection coefficients can be extracted by measuring light intensity at various position. Quantum interpretation of this experiment can also describe the experimental results. The counter intuitive picture of single photon transmission can be explained by the probability relation based on quantum mechanics. This work reveals the quantitative non-ideal results of the simple Gedanken experiment with photons. Figure 1. (a) A photo of the experimental setup. It consists a 532-nm laser diode, three linear polarizers (P1, P3, and P2), a silicon photodetector and an optical power meter. (b) The corresponding schematic of the setup shown in (a). (c) and (d) the experimental results without and with the polarizer P3 when the polarization angles 2 and 3 are rotated, respectively. 293 The 44 th Congress on Science and Technology of Thailand (STT 44)

300 F_002_PF: HOLOGRAPHIC CONDUCTIVITY OF MAXWELL FIELD COUPLED TO A SCALAR FIELD IN SCWARZSCHILD AdS SPACETIME Suphot Musiri* Department of Physics, Faculty of Sciences, Srinakharinwirot University, Bangkok 10110, Thailand * suphot@g.swu.ac.th Abstract: We analytically calculate the conductivity of Maxwell field, coupled to a scalar field in a planar Schwarzschild anti-de Sitter spacetime near the Breithenlohner-Freeman bound. We find the conductivity diverting at certain frequencies of the vector potential, implying holographic superconductivity. Our analytical approximation is in good agreement with the numerical result for small frequencies. 294 Science and Technology in the Disruptive Era

301 F_003_PA: ANALYSIS ISOCLINIC FRINGE PATTERN OF PMMA BY USING PLANE REFLECTION POLARISCOPE Yongyut Manjit 1* Apichart Limpichaipanit 2 and Athipong Ngamjarurojana 2 1 Faculty of Science at Si Racha, Kasetsart University Si Racha Campus, Chon Buri 20230, Thailand 2 Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand * yut_manjit@hotmail.com Abstract: In this research, we studied the isoclinic fringe pattern of thickness and a diagonal length of square shape 0.6 x2.5 cm. The sample was pressed by two-point loading of hydraulic press system N at the tip of a square shape. The isoclinic fringe pattern appeared on the sample. So, we can record the data of angle principal stress at first quadrant of the sample. According to the observation of isoclinic fringe pattern by the plane reflection polariscope, we can use the data of angle principal stress to analyzed the transmission of force into the sample by mapping trajectory. It was found that the angle of principal stress was zero at the diagonal line of the sample that depended on the direction of external force. The angle principal stress increased from diagonal line at center to the edge of sample because the shear stress produced in every area but did not produce at diagonal line of sample. The maximum concentration of trajectory produced around contact area zone of sample and decreased in vertical direction from contact area to the diagonal line of sample. Keywords: Plane Reflection Polariscope ; Stress trajectory; PMMA; Square 295 The 44 th Congress on Science and Technology of Thailand (STT 44)

302 F_004_PF: INFLUENCE OF STOICHIOMETRIC COMPOSITION ON STRUCTURAL, OPTICAL AND ELECTRICAL PROPERTIES OF In 2S 3- DOPED CdS xse 1-x THIN FILMS PREPARED BY VACUUM THERMAL EVAPORATION METHOD Montree Hankoy, 1,* Ngamnit Wongcharoen, 1 Chaisingh Poo-Rakkiat 2, Thitinai Gaewdang 1 1 Department of Physics, Faculty of Science, King Mongkut s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 2 Department of Physics, Faculty of Science, Chulalongkorn University, Phayathai Rd., Pathumwan, 10330, Thailand * montreehankoy@gmail.com Abstract: Thin films of In 2S 3- doped cadmium sulfoselenide (CdS xse 1-x) 0.99(In 2S 3) 0.01 (0 x 1) were fabricated by vacuum thermal evaporation method on glass substrates at a vacuum of 5x10-5 mbar. The as-deposited (CdS xse 1-x) 0.99(In 2S 3) 0.01 thin films were characterized their structural, morphological, optical and electrical properties. X-ray diffraction (XRD) results suggested that all prepared films exhibited single phase with hexagonal wurtzite crystal structure and the grain size in the range of nm calculated using the Scherrer s equation from (002) diffraction plane. The surface morphology and elemental compositions of the films were studied by AFM, FESEM and EDS, respectively. The optical transmission spectra were investigated by means of UV-Vis spectroscopy in the range of nm. The optical band gap value of the films increased from 2.00 to 2.48 ev as composition of S increased from 0 to 1.0 and giving the bowing parameter (b) = 0.47 ev. The electrical properties were carried out by resistivity and Hall effect measurements with van der Pauw configuration. The high deposition rate for vacuum thermal evaporation is an important advantage of this method which has potential use for high efficiency and low-cost solar cell manufacturing. 296 Science and Technology in the Disruptive Era

303 F_005_PF: QED CORRECTION OF LOW ENERGY PHOTON-PHOTON SCATTERING CROSS SECTION Suppanat Supanyo, 1 Udom Robkob 2 Department of Physics, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi District, Bangkok 10400, THAILAND 1 suppanatsupanyo@gmail.com 2 udom.rob@mahidol.ac.th Abstract: The QED photon-photon scattering cross section is calculated in the limit that photon energy is very small comparing to electron mass by using helicity amplitude method and the OPP s reduction. Our result is expressed in the power series of ω 2n+4 /m 2n+6, according to series expansion of master integral. The first term is comparable to the result from Euler-Heisenberg effective field theory, and the second and third order terms are comparable to the result from Dicus, Kao, and Repko which are also derived from effective field theory. The difference appears in the third order, in which the result from Dicus, Kao, and Repko is 1.94 times larger than our result. 297 The 44 th Congress on Science and Technology of Thailand (STT 44)

304 F_006_OF: EFFECT OF ANNEALING TEMPERATURE ON THERMOELECTRIC PROPERTIES OF GERMANIUM ANTIMONY TELLURIDE THIN FILMS PREPARED BY PULSED DC MAGNETRON SPUTTERING Intira Nualkham 1, Rachsak Sakdanuphab 2, Mati Horprathum 3, Aparporn Sakulkalavek 1 * 1 Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Road Ladkrabang, Bangkok 10520, Thailand 2 College of Advanced Manufacturing Innovation, King Mongkut s Institute of Technology Ladkrabang, Chalongkrung Road Ladkrabang, Bangkok 10520, Thailand 3 National Electronics and Computer Technology Center, National Science and Technology Development Agency, Pathum Thani 12120, Thailand * aparporn.sa@kmitl.ac.th Abstract: In this work, GeSbTe (GST) thin films were prepared by pulsed DC magnetron sputtering technique from a Ge 2Sb 2Te 5 target. The effects of annealing temperature on microstructural, electrical and thermoelectrical properties of GST thin films were investigated. The annealing process was employed in vacuum furnace under argon gas flow at different annealing temperatures. The as-deposited films show the non-stoichiometric composition of the target, resulting from the different sputtering yields of the Ge, Sb, and Te. As-deposited GST thin films exhibited amorphous phase and then changed to polycrystalline phase after annealing at the temperature of 200 C. The annealing process drive a phase transition to metastable cubic phase that played important role in enhancing the Seebeck coefficient. The maximum electrical conductivity and power factor of 10 5 S/cm and W/mK 2, respectively were obtained at annealing temperature of 250 C. 298 Science and Technology in the Disruptive Era

305 F_007_OF: DENSITY FUNCTIONAL THEORY STUDY OF STRAIN-INDUCED BAND GAP TUNABILITY OF TWO-DIMENSIONAL LAYERED MoS 2, MoO 2, WS 2 AND WO 2 Rutchapon Hunkao 1, Aniwat Kesorn 1, Ryo Maezono 2, Asawin Sinsarp 1, Worasak Sukkabot 3, Kritsanu Tivakornsasithorn 1 and Sujin Suwanna 1, * 1 Optical and Quantum Physics Laboratory, Department of Physics, Faculty of Science, Mahidol University, Bangkok 10400, Thailand 2 School of Information Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa , Japan 3 Department of Physics, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand * sujin.suw@mahidol.ac.th Abstract: Employing the density functional theory (DFT), we calculate the band gaps of two-dimensional layered materials MoS 2, WS 2, MoO 2, and WO 2 under uniaxial and biaxial strains. The band gap tunability of these materials is investigated. It is found that the band gap modulation is more sensitive to the biaxial strain, consistent with experimental results on MoS 2. Furthermore, we also find that a wide range of band gap can be achieved by applying strains ranging from -10% to 10%, for which the compressive strain and tensile strain show asymmetry. For small strain, MoS 2 and WS 2 exhibit smaller band gap energy as the tensile strain increases, but larger band gap energy as the compressive strain increases. For large strain, the MoS 2 and WS 2 band gap energies are reduced as both the tensile and compressive strains increase. A different behavior is observed for MoO 2 and WO 2 as their band gap energies are always reduced by the tensile strain, and enhanced by the compressive strain. Under the biaxial strain, MoO 2 and WO 2 can exhibit direct band gaps, indirect band gaps and become metallic for large enough tensile strains, 5% for MoO 2 and 7% for WO The 44 th Congress on Science and Technology of Thailand (STT 44)

306 F_008_OA: RADIOLOGICAL HAZARD ASESSMENT AND EXCESS LIFETIME CANCER RISK EVALUATION IN SURFACE SOIL SAMPLES COLLECTED FROM KLAENG DISTRICT IN RAYONG PROVINCE, THAILAND Prasong Kessaratikoon, 1, *, Nitsupa Luksum, 2 Ruthairat Boonkrongcheep, 1 Sitthipong Polthum 3 1 Nuclear and Material Physics Research Unit (NuMPRU), Department of Basic Science and Mathematics, Faculty of Science, Thaksin University, Songkhla Campus, Muang, Songkhla, 90000,Thailand 2 Undergraduate Student, B.Ed. (Science - Physics), Faculty of Education, Thaksin University, Songkhla Campus, Muang, Songkhla, 90000, Thailand 3 Nuclear Scientist, Thailand Institute of Nuclear Technology (Public Organization) 9/9 Moo 7 Saimoon Sub-District, Ongkharak District, Nakhon Nayok, 26120, Thailand * prasong_mi@hotmail.com Abstract: Specific activities of natural ( 40 K, 226 Ra and 232 Th) and anthropogenic ( 137 Cs) radionuclides in 43 surface soil samples collected from Klaeng district in Rayong province in eastern region of Thailand, have been studied and measured. A high-purity germanium (HPGe) detector and gamma spectrometry analysis system were employed to carry out all experimental results. The median values of the specific activities of 40 K, 226 Ra, 232 Th and 137 Cs were studied and calculated from the asymmetrical distribution in all surface soil samples and equal to ± 8.24, ± 1.03, ± 0.51 and < 0.87 Bq/kg, respectively. Four radiological hazard indices which are gamma-absorbed dose rate (D), radium equivalent activity (Ra eq), external hazard index (H ex) and annual external effective dose rate (AED out), were evaluated for the investigated area by using the median values of specific activities of 40 K, 226 Ra and 232 Th. Furthermore, the excess lifetime cancer risk evaluation (ELCR(outdoor)) could be calculated by using the AED out value. Moreover, the results were also compared with the Office of Atoms for Peace (OAP) annual report data, Thailand and global radioactivity measurement and calculations. From this study, the corresponding annual external effective dose rate (AED out) and the excess lifetime cancer risk evaluation (ELCR(outdoor)) were equal to 0.04 ± 0.01mSv/y and (0.14 ± 0.01) 10-3, respectively. These two values were lower than the worldwide average. The meaning is that the surface soil in the investigated area are unlikely harmful to human health. 300 Science and Technology in the Disruptive Era

307 SESSION G: MATHEMATICS / STATISTICS / COMPUTER SCIENCE 301 The 44 th Congress on Science and Technology of Thailand (STT 44)

308 G_001_PA: CLASSIFICATION OF SEASONS IN EACH REGION BY FILTER - FUZZY LOGIC METHOD Anan Sirithanyarat, 1 Somprat Srithagon, 2 12 Weather Forecaster, Weather Forecast Division, Weather Forecast Bureau, Thai Meteorology Department, Thailand. 1 mangomangrow@hotmail.co.th 2 srithagon.somprat@gmail.com Abstract: The main task of the meteorological department is forecasting with accuracy in order to support people as daily life, agricultural planning, fishery and disaster management. Disaster management, especially, types of disaster often relate to season period. So, if clearly classify seasons lead to effective disaster management. Today, 3 seasons in Thailand have been classified by the Department of Meteorology and wind direction is main criteria for being season indicator. Two problems of season classified in Thailand are i) the wind direction cannot reflect season weather in some local area, for example, Northeast monsoon (Winter monsoon) brings cold and dry weather from Tibetan Plateau to Upper Thailand. But, when it prevails pass Gulf of Thai which blew moisture from sea to cover southern of Thailand especially in the east coast cause to rain and ii) the use of standard seasons throughout the country may not relate actual weather conditions in some local areas. So, Filter - Fuzzy Logic Method were created by the concept local season periods must relate actual weather in local area. This research had 2 main purposes: i) to create seasonal classification of 6 chosen weather stations in 6 provinces (represented by six regions) namely Chiang Mai, Khon Kaen, Nakhon Sawan, Bangkok, Phuket, and Songkhla with standardized mathematical methods; and ii) to classify seasons in such provinces according to their actual circumstances. Input data of Filter - Fuzzy Logic Method uses 4 observed elements which are the daily maximum and minimum temperature, the relative humidity and rainfall quantity in 6 provinces since Filter - Fuzzy Logic Method has 2 tools, Filter (95-100%) and Fuzzy Logic. Filter was a tool for choosing the right day for each season. In other words, lowest temperature and humidity day must be in Cold Season period. Highest temperature day must be in Hot Season period. Highest maximum rainfall day must be in Rainy Season period. Sometimes, output of Filter (95-100%) for lowest temperature, highest temperature and highest maximum rainfall were occurred in the same day. Fuzzy Logic was a tool for selecting the day for each local actual season period. The results could remarkably indicate the beginning as well as the ending periods of Cold Season for 6 chosen provinces. But, the beginning as well as the ending periods of Hot Season and Rainy Season could be fairly indicated for certain provinces: Chiang Mai, Khon Kaen, Nakhon Sawan, and Bangkok. In case of Phuket and Songkhla, their weather patterns could be classified just into 2 seasons which corresponded to their exact situations. In the future, this method can be developed to be used as a basis for the announcement of the beginning day of the season in each province. Figure 1. Comparative of Seasonal Characteristic Periods for 6 Chosen Provinces Remark No.1 : Hot Season Period, No.2 : Rainy Season Period and No.3 : Cold Season Period 302 Science and Technology in the Disruptive Era

309 G_002_OF: DEVELOPMENT OF APPLICATION FOR TRAFFIC SIGN DETECTION IN PANORAMIC IMAGES USING DEEP LEARNING TECHNIQUE Sathit Prasomphan*, Thanthip Tathong, Primpisa Charoenprateepkit Department of Computer and Information Science Faculty of Applied Science, King Mongkut s University of Technology North Bangkok, 1518 Pracharat 1 Rd. Wongsawang, Bangsue, Bangkok, THAILAND * sathit.p@sci.kmutnb.ac.th Abstract: A new approach and application for detecting the signs inside a large panoramic image was considered in this research. A method for panoramic traffic sign images detection for regulatory signs and guide signs especially blue and green sign was proposed. For a detecting process, a convolution neural network technique was used. The technique used in conjunction with the convolution neural network technique by using Tensorflow training to improve the accuracy of traffic sign detection are required. Some image processing technique, for example, adding brightness to an image, was added to enhance the accuracy of process to image detection. The experimental results showed that detection of traffic signs from panoramic images (360º) by using trained convolution neural network model and image processing technique improve a traffic sign detection. The accuracy from panoramic images (360º) detection using the proposed method is better than the traditional model. 303 The 44 th Congress on Science and Technology of Thailand (STT 44)

310 SESSION H: FOOD SCIENCE & TECHNOLOGY 304 Science and Technology in the Disruptive Era

311 H_001_PA: PRODUCTION OF LOW FAT ICE CREAM FROM BLACK GLUTINOUS RICE MILK Narin Charoenphun 1, * and Kanokporn Pakeechai 2 1 Faculty of Agricultural Technology, Burapha University Sakaeo Campus, Sakaeo Faculty of Business Administration and Information Technology, Rajamangala University of Technology Suvanabhumi, Ayutthaya * narinch@buu.ac.th Abstract: Ice cream is a frozen dairy product. It is popular in the widespread consumption. However, some consumers lacking lactase enzyme in the intestine or there are allergic to milk protein. As a result, they are not able to consume dairy products. This research aims to develop healthy ice cream products that are suitable for cow milk allergy consumers, who are allergic to cow milk. The optimum formula for producing ice cream from milk rice consisting of rice berry, black glutinous rice and rice berry mixed with black glutinous rice (50:50) as compared with milk ice cream (control) were studied. Physical properties and sensory evaluation by 9-point hedonic scales of ice cream were investigated. The result showed that the control formula had the highest percentage of overrun as followed by rice berry formula. Interestingly, black glutinous rice formula had higher overall liking score (7.73±0.88) than riceberry and mixed formulas. Therefore, black glutinous rice formula was selected for studying in the replacement of dairy whipping cream by using cavendish banana, sunchoke, soy milk and glucose syrup. The result indicated that cavendish banana replacement in dairy whipping cream formula had the highest overall liking score (7.50±0.90). Apparently, the overrun, viscosity and melting rate were 26.40±0.15 (%), ±3.33 (cp) and 0.08±0.01 (g/min), respectively. Fat content of cavendish banana formula was 1.06% lower than that of dairy whipping cream formula (5.87 %). Therefore, rice milk ice cream reducing fat content are good as healthy food products and suitable for milk allergy consumers. 305 The 44 th Congress on Science and Technology of Thailand (STT 44)

312 H_002_PF: MODIFICATION OF EGG ALBUMEN FILM BY THE MAILLARD REACTION Pinyapat Jitphongsaikul, Thanachan Mahawanich* The Novel Technology for Food Packaging & Control of Shelf Life Research Group, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, 10330, Thailand * Abstract: Modification of protein film properties can be achieved using various techniques, including cross-linking via the Maillard reaction. The objective of this study was to investigate the effect of type and concentration of reducing carbohydrates on properties of Maillardmodified egg albumen film. Reducing carbohydrates, namely glucose, maltose, 10DE maltodextrin, and 18DE maltodextrin, were used in this study. Egg albumen-to-reducing carbohydrate ratio was varied at 1:2, 1:4, and 1:8. Tensile strength (TS) of the Maillard-modified film was found to increase, while elongation at break decreased, with increasing reducing carbohydrate concentration. Glucose was proved to be the most efficient in terms of improving TS of the egg albumen film. In terms of optical properties, all film samples exhibited a similar hue angle of approximately 90 but increasing chroma value was demonstrated at high concentrations of reducing carbohydrates while changes in transparency showed an inconsistent trend in the Maillard-modified films. A decrease in water vapor permeability and water solubility was signified in the Maillard cross-linked films. 306 Science and Technology in the Disruptive Era

313 H_003_PA: THE PASTEURIZATION OF MILK AND LACTOSE-FREE MILK APPLYING OHMIC HEATING IN COMPARISON WITH CONVENTIONAL METHOD Napat Suebsiri, Pasawut Kokilakanistha, Titti Laojaruwat, Titaporn Tumpanuvat and Weerachet Jittanit* Department of Food Science and Technology, Faculty of Argo-Industry, Kasetsart University, 50 Ngam Wong Wan Road, Lat Yao, Chatuchak, Bangkok 10900, Thailand * Abstract: So far, there has been an increasing number of people with lactose intolerance reported in Thailand. In this study, the lactose-free milk was prepared from the cow milk using lactase enzyme. The electrical conductivities of both normal and lactose-free milks obtained from various conditions were measured applying ohmic heating apparatus. Furthermore, the chemical and physical properties of milk samples pasteurized at 63 C for 30 min by conventional method and ohmic method were compared. The main objective was to determine the potential of applying ohmic method in the pasteurization of both normal and lactose-free milks. It appeared that the electrical conductivities of milk samples were in the range of S m -1 indicating that they could be efficiently heated by ohmic method. Moreover, the electrical conductivities of normal and lactose-free milks were insignificant different. There was no significant difference in aspects of protein content, total acidity and specific gravity between the milk samples pasteurized by conventional and ohmic methods. In summary, the ohmic method showed its potential for the milk pasteurization. 307 The 44 th Congress on Science and Technology of Thailand (STT 44)

314 H_004_PF: SENSORY AND THE PHYSICOCHEMICAL PROPERTIES OF DEVELOPED KLUAI HIN SMOOTHIE AS AN ALTERNATIVE PRODUCT FOR THE LOCAL FARMER AND THE HEALTH CARE CONSUMER Fateehah Bueto 1, Nadia Doloh 2 and Patthamawadee Tongkaew 3,* 1,2.3 Department of Food Science and Nutrition, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand * patthamawadee.c@psu.ac.th Abstract: Due to no variation of Kluai Hin products in the market, the objective of this study was to develop a new beverage product, Kluai Hin smoothie. Four smoothie recipes with banana as the main ingredient were prepared and the most acceptable recipe was selected. In addition, the ratio of Kluai Hin pulp in water or the whole milk base at difference levels; 20:80, 30:70, 40:60, 50:50 and 60:40 (g/ml) followed by pasteurization at 75 C for 1 minute were studied. Furthermore, the sensory analysis and the physicochemical properties were investigated. The result showed that Kluai Hin with the whole milk base provided the smoothest consistency. Additionally, at the ratio of 50:50 g/ml (Kluai Hin pulp: whole milk) revealed the highest acceptable for consumers. For physicochemical analysis, the TSS of Kluai Hin smoothie was the highest content (21.00 ± 0.00 Brix) compared to other products. Additionally, the ph, the viscosity and the TTA of Kluai Hin smoothie were 3.79 ± 0.04, ± cp and 1.57 ±0.13 g citric /100 ml, respectively. The color of L*, a* and b* were 69.5±0.06, 5.76±0.13 and 40.24±0.22, representing the contribution of yellow from natural ingredients. In conclusion, the developing Kluai Hin smoothie meets the standards of Brix of FDA and the notification of Ministry of Public Health. This beverage might be an alternative product for a local producer and health care consumer. 308 Science and Technology in the Disruptive Era

315 H_005_OF: PHYSICOCHEMICAL, PHENOLIC COMPOUNDS AND IN VITRO DIGESTIBILITY OF THAI PURPLE RICE VARIETY INFLUENCED BY GERMINATION CONDITIONS Iyiola Oluwakemi Owolabi, Bandhita Saibandith, Santad Wichienchot, Chutha Takahashi Yupanqui * Interdisciplinary Graduate School of Nutraceutical and Functional Food (IGS-NFF), Prince of Songkla University, Hat-Yai, Songkhla, Thailand * chutha.s@psu.ac.th Abstract: The proximate composition, some bioactive compounds, antioxidant activities and in vitro enzymatic hydrolysis of starch, at different germination conditions of germinated Thai purple rice (Khau Neaw Dam) were evaluated in this study. Comparing different germination times (0 h, 12 h, 24 h and 48 h); germination at 12 h provides the purple rice with nutritionally superior quality on the basis of its lower reducing sugar and bioactive compounds (phenolics, anthocyanin), increased antioxidant activity and reduced glucose contents released during digestion. Overall, germination seems to be a natural and sustainable innovative technique in improving the nutritional quality, phenolic and producing grains with lower glucose contents. 309 The 44 th Congress on Science and Technology of Thailand (STT 44)

316 H_006_PA: MICROBIOLOGICAL SURVEY OF COLIFORMS AND Escherichia coli IN LEAFY GREENS FROM PUBLIC MARKET Tumnoon Charaslertrangsi 1, *, Niva Sthapit 2, Yin May Tun 2, Kessaya Vorapiboonvit 2, Woranon Leevongcharoen 3 1 Biological Sciences Program, Science Division, Mahidol University International College, 999 Phutthamonthon, Salaya Phutthamonthon, Nakhon Pathom, 73170, Thailand 2 Undergraduate Program in Biological Sciences, Mahidol University International College, 999 Phutthamonthon, Salaya Phutthamonthon, Nakhon Pathom, 73170, Thailand 3 Undergraduate Program in Food Science and Technology, Mahidol University International College, 999 Phutthamonthon, Salaya Phutthamonthon, Nakhon Pathom, 73170, Thailand * tumnoon.cha@mahidol.ac.th Abstract: Microorganisms are naturally present on foods, and can be inactivated during the cooking process. Fruits and vegetables however are often consumed raw, and thus have been associated with many foodborne outbreak incidents. Therefore, in this study, five leafy green produce, namely spearmint (Mentha spicata), lettuce (Lactuca sativa), coriander (Coriandrum sativum), Chinese cabbage (Brassica rapa subsp. pekinensis), cucumber (Cucumis sativus), from local market in Bangkok metropolitan area (N=50) were examined. Using most probable number method, spearmint, lettuce, coriander, Chinese cabbage, and cucumber were contaminated with a mean coliform count of , , , , MPN/g, respectively, after being rinsed with tap water to mimic household circumstances. Using selective agars and biochemical testing, 28 coliform isolates were obtained, six of which were Escherichia coli. Using universal primer to amplify V3-V6 region of 16S rrna gene and comparing the amplicons to NCBI BLAST database, the isolates include Klebsiella spp., Citrobacter freundii, and Enterobacter spp. The E. col isolates were further tested for virulence genes: Stx1, Stx2, +93 uida, -eaea, and ehxa. Results showed three isolates tested positive for Stx1, while all six isolates were negative for Stx2. Two isolates tested positive for intimin ( eaea) gene, while an isolate was positive for enterohaemolysin gene, ehxa. In conclusion, the coliforms and the enteropathogenic strains of E. col in leafy greens may inflict health hazard to the public upon exposure. 310 Science and Technology in the Disruptive Era

317 H_007_PA: A REAL-TIME LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR RAPID DETECTION OF Salmonella spp. in FOOD SAMPLES Narong Arunrut, 1, * Wansika Kiatpathomchai 1 and Chiraporn Ananchaipattana 2 1 Bioengineering and Sensing Technology Laboratory, BIOTEC, National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Rd., Khlong Neung, Khlong Luang, Pathum Thani, Thailand 2 Department of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, 39 Muh1, Thanyaburi, Pathum Thani, Thailand * Narong.aru@biotec.or.th Abstract: Salmonella spp. is the most commonly identified bacterial cause of foodborne disease and its presence in chicken meat products poses a risk to public health. Conventional methods for the detection of Salmonella spp. based on culturing are time consuming and laborious. Thus, a rapid, sensitive and quantitative assay using Real-time Loop mediated isothermal amplification (real-time LAMP) was developed for Salmonella detection. Gene was the target sequence amplified by Real-time LAMP assay. The LAMP assay did not show crossreactivity with several common bacterial pathogens and the detection limit was 1.2 CFU/mL for genomic DNA in pure culture. Spiked samples simultaneously detected 7 CFU/mL of Salmonella pathogen in artificially inoculated samples after enrichment for 6 h. Compared to standard culture-based methods the sensitivity, accuracy and specificity test of real-time LAMP assay for 120 raw chicken meat samples were 94.02, and 86.79% respectively. Results demonstrated that this method is an accurate, sensitive and specific and can be used for rapid detection and differentiation of foodborne diseases. Figure 1. A schematic diagram showing the step to detect Salmonella spp. in food samples by real-time LAMP assay. 311 The 44 th Congress on Science and Technology of Thailand (STT 44)

318 H_008_PF: THE EFFECT OF ULTRASOUND ON MASS TRANSFER AND TEXTURE OF OSMOTIC DEHYDRATED COCONUT Saowanee Lertworasirikul,* Nitchakarn Sumsiripong, Manassawee Kuhawijit Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Thailand * Abstract: An important problem of osmotic dehydrated coconut product dried with hot-air is the hard texture of product. This research was to study the effect of ultrasound (US) pre-treatment on water loss and solid gain during osmotic dehydration (OD), and moisture transfer and hardness of dried coconut during air-drying. The coconut samples were immersed in sucrose solution (65% w/w) and subjected to different 40 khz ultrasound treatments (0, 10, 20 and 30 min) before blanching for 1 min, cooling to room temperature, and OD in a shaking water bath at 50 rpm, 28±1ºC for 6 h. The results revealed that the US treatment affected on WL but not SG of coconut. Higher WL was observed when using US; however, 20 and 30 min of US did not affect WL differently. The samples after US and OD treatments were then dried in a hot-air dryer at 65ºC for 10 hrs. It was found that 10 min US, 1 h OD and 3 h drying was the optimal processing condition for the best texture of coconut product and shortest processing time. Sensory evaluation showed that appearance, color, texture, sweetness, and overall liking from 9- pointed hedonic scale of the sample were above 7 (like moderately). 312 Science and Technology in the Disruptive Era

319 H_009_PF: EFFECT OF STORAGE CONDITIONS ON SENSORY AROMA CHARACTERISTICS OF KHAO DAWK MALI-105 BROWN RICE Ruchirus Muthikul, 1 Supeeraya Arsa, 2 Chockchai Theerakulkait 3, * 1 Food Science and Technology Department, Faculty of Science, Phranakhon Rajabhat University, Bangkok 10200, Thailand 2 Faculty of Agro-Industry, King Mongkut s Institute of Technology Ladkrabang, Bangkok 10520, Thailand 3 Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand * fagicct@ku.ac.th Abstract: Khao Dawk Mali-105 (KDML) is the most important aromatic rice cultivar in Thailand. Storage condition is an important factor that might influence on an aroma of fragrant rice. Therefore, the aim of this study was to investigate the effect of storage conditions; including light exposure, storage temperatures and times, on sensory aroma characteristics of KDML fragrant brown rice (KBR). KBR was kept in linear low density polyethylene (LLDPE) bag at room temperature with light exposure and in the dark at room temperature (25±2 C) and at refrigerator (4±2 C) for 8 months. Aroma descriptive analysis of KBR after storage for 0, 2, 4, 6 and 8 months were determined. The light exposure did not significantly influence on pandanlike and pounded unripe rice (khao-mao)-like aromas of uncooked KBR. In contrast, light exposure significantly increased the rancid and musty odors of cooked KBR after storage for 6 and 8 months. The pandan-like and khao-mao-like aromas of uncooked KBR and cooked cornlike aroma of cooked KBR could be preserved by storing at dark and low temperature in refrigerator. The intensity of pandan-like and khao-mao-like aromas of KBR that stored at room temperature with light exposure decreased while rancid and musty odors increased as longer storage time. These results indicate that light exposure, storage temperature and time influenced on the sensory aroma characteristics of KBR. These findings indicated that storage conditions are the crucial factors that should be concerned in order to preserve aroma quality of brown rice during storage. 313 The 44 th Congress on Science and Technology of Thailand (STT 44)

320 H_010_PA: ANTIOXIDANT ACTIVITIES OF CRUDE EXTRACTS OF EDIBLE MUSHROOM COLLECTED FROM CHIANG RAI PROVINCE Suchawadee Insawang 1 Patcharee Pripdeevech 1,2,* 1 School of Science, Mae Fah Luang University, Chiang Rai, Thailand 2 Center of Chemical Innovation for Sustainability (CIS), Mae Fah Luang University, Chiang Rai, Thailand * patcharee.pri@mfu.ac.th Abstract: Mushrooms have a long tradition of use in Northern Thailand. Edible mushrooms are food full of proteins, vitamins, minerals and essential amino acids. Mushrooms have been reported as natural antioxidant extract. Objective of this work was to evaluate the antioxidant properties of three edible mushroom species Thaeogyroporus porentosus, Russula sanguineus and R. delica collecting from Chiang Rai province on May For determination of potential antioxidant activity, antioxidant activity by using 2,2-diphenyl-1-pricylhydrazyl radical scavenging assay has been evaluated. Fresh mushroom samples were dried in hot air oven at 60 C for two days and further macerated in different extraction solvents; dichloromethane, hexane and ethyl acetate for five days. The highest extraction yield was achieved in hexane extract of R. sanguineus (0.316 %w/w). Antioxidant activity was determined to exhibit IC 50 value for all crude extracts. Moderate antioxidant activity was detected among all crude extracts with IC 50 ranging from mg/ml (Fig. 1). Crude extract of R. sanguineus from hexane maceration was evaluated to contain the highest antioxidant activity (4.27 mg/ml) which was similar to those obtained by trolox, reference compound, representing IC 50 of 0.34 mg/ml. These results indicated that hexane extract of R. sanguineus has a potential to be natural antioxidant source. Figure. 1 IC 50 value of crude extracts of T. porentosus, R. sanguineus and R. delica obtained from different extraction solvents. 314 Science and Technology in the Disruptive Era

321 H_011_PA: PREVENTION OF BROWNING FORMATION IN JASMINE FLOWER (Jasminum sambac) DURING POSTHARVEST STORAGE Chomphunut Wiriyawatthana, Supawadee Somkane, Nilobon Komonsing, Pramote Khuwijitjaru, Busarakorn Mahayothee* Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, Thailand * Abstract: Jasmine flower is known as a fragrance flower. Jasmine flower buds is commercially available in domestic market for religious ceremonies, medicinal purposes and tea production. Its major problem during postharvest storage and transportation is a short shelf-life due to discoloration and early opening of the flower buds. Thus, the aim of this study was to investigate the effect of calcium chloride combined with ascorbic acid or citric acid or sodium citrate on retarding browning formation in jasmine flower buds during storage. Two lots of fresh jasmine flower bud with the ph value of 5.32 without any visual defects were harvested in the afternoon hour from a farm in Ratchaburi province, Thailand. They were immediately immersed in cold water, drained, packed in polyethylene bags and kept in a box containing ice during transportation to the laboratory. After that, they were secondary washed with cold water (10 ± 2 C) to clean and to retain flower firmness. The jasmine flower buds were drained before dividing into six groups for six different solutions treatments including water (control), 0.5% calcium chloride, 0.5% calcium chloride with 0.5% citric acid, 0.5% calcium chloride with 0.5% ascorbic acid, 0.5% calcium chloride with 0.5% sodium citrate and 0.5% calcium chloride with 0.5% citric acid and 0.5% ascorbic acid. The ph values of solutions were 6.73, 7.68, 2.39, 2.91, 7.22 and 2.34, respectively. They were dipped in the solutions for 10 min at ambient temperature (25 ± 2 C). After that, the jasmine flower buds were drained prior to packing in a sealed polyethylene bag. Each bag contained 50 g of samples. Two bags were prepared for each treatment per day of testing. The experiments were performed in duplicate. Samples were kept in a box containing ice (3 ± 1 C) for 10 days. Browning index, color (L*, a*, b* and E) and ph values were measured at 0, 2, 4, 6, 8 and 10 days of storage. It was found that the browning formation due to oxidative damages of the tissues occurred faster than the opening of flower buds. No significant difference of ph values of jasmine flower buds found between each experiment (p > 0.05). Jasmine flower buds dipped in 0.5% calcium chloride with 0.5% citric acid and 0.5% ascorbic acid showed a better quality in terms of color and retarding of browning formation during storage compared to other treatments. It can be kept for 10 days with the L*, a*, b* and E values of ± 0.13, ± 0.27, ± 0.18 and 6.80 ± 0.18, respectively. 315 The 44 th Congress on Science and Technology of Thailand (STT 44)

322 H_012_PA: EFFECT OF SEVERITY FACTOR ON SUBCRITICAL WATER EXTRACTION OF OLIGOSACCHARIDES FROM PASSION FRUIT PEEL Thaweesak Tana, Pramote Khuwijitjaru * Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand * khuwijitjaru_p@su.ac.th Abstract: The weight of passion fruit peel can be as high as 50% of the whole fruit. The peel is rich in polysaccharides which can be hydrolyzed into higher-value oligosaccharides. In this research, passion fruit peel was treated in subcritical water under non-isothermal conditions in a batch-type reactor. The dried peel and water in the ratio 1:16 (w/v) was heated to the maximum temperatures in the range of C. The effects of the treatment temperature and time were combined and expressed as severity factor (ln R 0) which were in the range of The highest total carbohydrate and reducing sugar contents (40.74 and g/100 g dry peel, respectively) were obtained at ln R 0 of 9.3 (180 C for 30 min). At higher ln R 0 value, higher quantity of 5-hydroxymethyl-2-furaldehyde was detected. The hydrolysates were also analyzed by high performance size exclusion chromatography (HPSEC) and the results showed that at ln R 0 of 9.3, the highest peak area in the molecular weight range of Da was obtained. The molecules in this molecular weight range can be regarded as oligosaccharides. Therefore, this research showed that subcritical water is potential method for converting polysaccharides in passion fruit peel into oligosaccharides. Figure 1. Temperature profiles during treatment (a) and HPSEC chromatograms of hydrolysates and carbohydrate standards obtained at different severity factors (b). 316 Science and Technology in the Disruptive Era

323 H_013_PA: UNRIPE GREEN BANANA FLOUR (Cavendish spp.): SOME PROPERTIES AND ITS APPLICATION Pakathip Thakaeng, 1 Thitirat Boonloom, 1 Orapan Romruen, 2 Saroat Rawdkuen 1,2 * 1 Food Technology Program, School of Agro-Industry, Mae Fah Luang University, Muang, Chiang Rai, Thailand 2 Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Muang, Chiang Rai, Thailand * saroat@mfu.ac.th Abstract: The purpose of this study was to determine physico-chemical and functional properties of unripe banana flour (UBF) from bananas (stage 1, all green of Cavendish spp.) that are off-specifications, comparing to commercial wheat flour (WF). The flours were mixed in different ratios (0-80%) for making bread and then some qualities attributes of the bread were determined. The results showed that UBF showed higher in carbohydrate content (85.81%) and total dietary fiber (7.11%) than WF, while protein (4.98%) and fat contents (0.34%) had lower than WF (P<0.05). UBF contained higher tannin, amylose content, and antioxidant activity. The microstructure of UBF was characterized by light microscopy and showed that starch granules in UBF were larger than those found in WF. XRD pattern of starch in UBF can classify as an A-type with 26.31% of crystallinity. Solubility, swelling power, and water absorption capacity of WF were greater than that UBF (P<0.05). The pasting properties obtained by RVA showed that UBF had higher in pasting temperature, peak viscosity, breakdown, and final viscosity than WF, while setback viscosity showed lower than WF (P<0.05). Increasing the level of UBF caused an increase in hardness of bread, and decrease in loaf volume. According to the results, value-added product can be developed with health-promoting properties by using UBF (up to 20%). The utilization of UBF as functional food ingredient in any food recipe could be benefit to the consumer, especially for requiring high dietary fiber and can be used as gluten-free products. 317 The 44 th Congress on Science and Technology of Thailand (STT 44)

324 H_014_OF: A PROTEASE-RESISTANT α-galactosidase FROM Paenibacillus polymyxa STRAIN PB5 (WITH GOOD HYDROLYTIC ACTIVITY TOWARDS RAFFINOSE FAMILY OLIGOSACCHARIDES) Sreyneang Nhim 1, Suratsawadee Tiangpook 2, Khanok Ratanakhanokchai 2, Chakrit Tachaapaikoon 1,2, Patthra Pason 1,2 and Rattiya Waeonukul 1,2* 1 Pilot Plant Department and Training Instituted, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand 2 School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand * rattiya.wae@kmutt.ac.th Abstract: The α-galactosidase gene, GH36A from Paenibacillus polymyxa strain PB5 was cloned and expressed in Escherichia coli BL21. The gene has an open reading frame of 2184 bp encoding 728 amino acid residues. The recombinant GH36A was purified by nickel affinity column chromatography 70% yield of the total activity from the crude extract of E. coli BL21. The purified GH36A showed a molecular mass of 80 kda in SDS-PAGE and a specific activity of 220 U/g proteins toward p-nitrophenyl α-d-galactopyranoside (pnpgal). The relative specific activity of GH36A towards various substrates is in order of pnpgal > stachyose > raffinose. The GH36A completely hydrolyzed raffinose and stachyose present in soybeans at 40 C within 60 min. Furthermore, the GH36A displayed remarkable resistance to protease. Especially, it exhibited strong resistance to pepsin with residual activity of 85% after incubation for 2 hour. These properties of GH36A make it useful in the food and feed industries for improving the nutritive value of soybean and other legumes by eliminating non-digestible flatulence-causing oligosaccharides RFOs. 318 Science and Technology in the Disruptive Era

325 H_015_PF: OPTIMIZATION OF COCONUT WATER POWDER BY USING SPRAY DRYING AND FOAM MAT DRYING METHOD: EFFECT OF DRYING CONDITIONS ON PHYSICO-CHEMICAL AND SENSORY PROPERTIES. Parichart Yamalee 1 * and Pisit Dhamvithee 2 12 Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Thailand. * parichart.yamalee@gmail.com Abstract: The effects of drying conditions on water activity (a w), moisture content, %yield, water solubility index (WSI), solubility, color, protein content, foam stability, foam density of spray dried and foam mat dried coconut water powder also sensory properties were investigated. For spray drying method, it was conducted in term of three factors: outlet air temperature (70, 80 and 90 C), inlet air temperature (140, 160 and 180 C) and concentration of maltodextrin (MAL) (20, 30 and 40 %w/w). Foam mat drying method, it was conducted in three factors: dry temperature (65 and 75 C), concentration of hydroxypropyl methylcellulose (HPMC) (0 and 1 %w/w) and 20%w/w of fresh egg white liquid (EWL): egg white powder (EWP) (100:0, 50:50 and 0:100 w/w). For spray drying, the results shown that the outlet air temperature maximum influenced on a w and moisture content while concentration of maltodextrin influenced on %yield. The optimum of spraying conditions were inlet air temperature 180 C, outlet air temperature C and concentration of maltodextrin %w/w respectively. For foam mat drying, the results shown that increasing in concentration of HPMC could lead foam stability increased. At 0%HPMC and ratio EWL: EWP 100: 0 (w/w) gave the highest %WSI. However, %WSI and solubility of coconut water powdered from spray drying was better than foam mat drying. As a results of sensory sorting task of coconut water powdered reconstitution in flavor aspect, several samples from spray drying and foam mat drying were grouped together with fresh coconut water due to those could stored aroma and/or flavor of coconut water powder similar with the fresh coconut water. 319 The 44 th Congress on Science and Technology of Thailand (STT 44)

326 H_016_PA: PROPERTIES OF BANANA (Cavendish spp.) STARCH FILM INCORPORATED WITH BANANA PEEL EXTRACT AND ITS APPLICATION Chanitda Taweechat, 1 Tipapon Wongsooka, 1 Wantida Homthawornchoo, 2 Orapan Romruen, 2 Pimonpan Kaewprachu, 2 Saroat Rawdkuen 1,2 * 1 Food Technology Program, School of Agro-Industry, Mae Fah Luang University, Muang, Chiang Rai, Thailand 2 Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Muang, Chiang Rai, Thailand * saroat@mfu.ac.th Abstract: The objective of this study was to develop an active banana starch film incorporated with banana peel extract. The film was compared its properties with commercial wrap film (Polyvinyl chloride; PVC). Moreover, the quality of wrapped minced pork during refrigerated storage was monitored. The banana starch film with different concentrations of banana peel extract [0, 1, 3, and 5 (%, w/v)] showed low mechanical properties [TS: MPa and EAB: (%)] and water vapor permeability [ ( g mm/sm2 Pa)]. The developed film also showed low film solubility [26 41 (%)], but excellent barrier properties to UV light. It had the thickness range of mm, and color attributes are L* = , a* = , b* = The developed starch-based film incorporated with banana peel extract 5 (%, w/v) showed the highest radical scavenging activity (97.9 μmol Trolox equivalent/g dried film), inhibitory activity of E. coli (O157: H7). The developed film showed some properties comparable to the commercial wrap film. Changes in qualities of minced pork were determined throughout the storage at 4 ± 1 C for 7 days. It was found that the sample wrapped with the developed film had low TBARS compared to that wrapped with the PVC. Successful inhibition of lipid oxidation in the minced pork was possible with the developed film. The banana starch-based film incorporated with banana peel extract could maintain the quality of minced pork and thus prolong its shelf-life. 320 Science and Technology in the Disruptive Era

327 H_017_PF: CHARACTERIZATION OF PROTEIN ISOLATE FROM SACHA INCHI PRESSED-CAKE BY USING THREE-PHASE PARTITIONING TECHNIQUE Atchara Maddolah, 1 Atchariya Leeyiamsakul, 1 Orapan Romruen, 1 Saroat Rawdkuen 1,2, * 1 Food Technology program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand 2 Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand * saroat@mfu.ac.th Abstract: Sacha inchi pressed-cake is the remaining after oil processing which contain high in protein content. The purpose of this study was to recover the proteins from Sacha inchi pressed-cake by using three-phase partitioning technique (TPP). The obtained proteins were subjected to hydrolyze and characterize in terms of protein digestibility, antioxidant activity, and protein patterns. Initial Sacha inchi pressed-cake contained the largest amount of protein (43.20%) and followed by carbohydrates (28.03%), fat (20.00%), ash (5.21%), and moisture (3.56%). The highest proteins recovery (98.66%) was obtained from the system consist of the ratio of crude protein extract to t-butanol of 1.0:2.0 in the present of 50% ammonium sulfate with 58.86% yield. The increasing crude protein extract to t-butanol ratio resulted in increased the protein obtained (P<0.05). In addition, when the concentration of ammonium sulfate increased, the protein recovery was also increased (P<0.05). SDS-PAGE profiles showed that the main Sacha inchi protein had MWs of 30 kda. The obtained protein from TPP was then subjected to hydrolyze by using crude papain (PA) and bromelain (BR). The resulting peptide was determined for total phenolic content (TPC), antioxidant activity (DPPH) and protein patterns (SDS-PAGE). The TPC content ranged from 0.27 to 1.03 µg GAE/mL and 0.43 to 0.96 µg GAE/mL with BR and PA, respectively, The DPPH ranged from 3.47 to 50.76% and 3.56 to 35.02% with BR and PA, respectively. Therefore, TPP could be used to recover the Sacha inchi protein with a high yield with antioxidant activity. 321 The 44 th Congress on Science and Technology of Thailand (STT 44)

328 H_018_OF: A COMPARATIVE STUDY OF SENSORY CHARACTERISTICS ON PINEAPPLE GROWN IN CHIANG RAI WITH DIFFERENT CULTIVARS AND CULTIVATION METHODS Chirat Sirimuangmoon 1 * 1 Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai, 57100, Thailand * chirat.sir@mfu.ac.th Abstract: Effects of cultivar and cultivation method on sensory characteristics of pineapple grown in Chiang Rai, Thailand were examined. Two cultivars of pineapple (Phu Lae and Nang Lae) were selected and were grown by farmers with different cultivation methods including cultivation with chemical fertilizers, cultivation with 100% organic fertilizers, and cultivation with natural condition. Commercial pineapples in both cultivars were also obtained from local markets. Cultivar played a critical role in the difference of sensory characteristics. Nang Lae pineapple expressed some sensory characteristics such as juicy texture, wet appearance, sour taste, and sour aroma whereas Phu Lae pineapple exhibited crunchy and dense texture, and deeper yellow color of flesh. Between these two cultivars, aromas, tastes, flavors and textures were all main significant sensory characteristics. Phu Lae pineapple had a higher intensity of pineapple aroma identity, sugary aroma, sweet aroma/flavor, honey aroma/flavor, ripe fruit aroma/flavor, sweet taste/aftertaste, crunchiness, etc. than those characteristics in Nang Lae pineapple. Applying chemical fertilizers also caused the major difference on the sensory characteristics. In Nang Lae cultivar, pineapples grown with chemical fertilizers trended to have sweeter taste, juicier texture, and deeper yellow color of flesh than pineapples grown with organic and natural conditions. Aromas, tastes, and flavors among Nang Lae pineapple samples were not significant difference. Phu Lae pineapple grown with chemical fertilizers had a higher intensity especially on the aromas, tastes, and flavors than those in Phu Lae pineapple grown with organic and natural conditions. Cultivar and cultivation method affected highly to sensory characteristics of pineapples. The results obtained from this study were useful to understand how the sensory characteristics differ when comparing with different cultivars and cultivation methods. The data would be used to relate with consumer acceptance data, to investigate the driver of liking, to see relationship between sensory and physio-chemical properties. 322 Science and Technology in the Disruptive Era

329 H_019_PF: DEVELOPMENT OF HIGH PROTEIN SNACK PRODUCT FROM RICEBERRY RICE, SOY AND EGG Pukchunya Siriwan* and Suteera Vatthanakul Department of Food Science and Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand * pukchunya@gmail.com Abstract: The object of this research was to study on effect of the ingredients by using Plackett and Burman design (N=12) for developed snack product. Six ingredients (Riceberry rice paste, soy milk, fresh egg, dried soy bean meal, egg white powder, and gum from basil seeds) were prepared for this study. The physical, chemical and sensory properties of snack product were determined. The result clearly showed that the protein content of snack product increased when added more fresh egg and egg white powder. The addition of dried soybean meal resulted in increased the hardness of the snack product. From sensory evaluation with 5 trained panelists in the term of hardness and crispness found that crispness of the 12 snack products, can divided into 2 groups (crumbly crispness and brittle crispness). The snack products which had much amount of rice were divided into crumbly crispiness group while the other which had much amount of egg white powder and dried soy bean meal were divided into the brittle crispness group. The best formula (highs protein) is riceberry rice paste %, soy milk %, fresh egg %, dried soy bean meal %, egg white powder 3.57 % and gum from basil seeds 3.57 %, respectively. Keywords: snack product, screening, Plackett and Burman design, crispness 323 The 44 th Congress on Science and Technology of Thailand (STT 44)

330 H_020_FP: EVALUATION OF SOLID-LIQUID EXTRACTION KINETICS OF ANTIOXIDANT, PHENOLIC AND SWEETENING COMPOUNDS FROM Stevia Rebaudiana LEAF Natthakan Rungraeng* Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai, Thailand * Abstract: Sweeteners from stevia leaf have been increasingly used as alternatives to table sugar. Stevioside and rebuadioside A are major glycosides found in stevia leaf. They are few hundred times sweeter than sucrose. Besides that, stevia leaf also contains health promoting phenolic compounds especially phenols and flavonoids. Extraction is a crucial technique for separating active compounds from plant matrix. This research aimed at determining release kinetic parameters for modeling the extraction of antioxidant, phenolic compounds, and sweeteners namely steviodioside and rebaudioside A from stevia leaf. The effects of leaf to water weight ratio, temperature and extraction cycle on the amounts of antioxidant activity (AA) using DPPH scavenging assay, total phenolic content (TPC) and steviodioside and rebaudioside A were evaluated. In the first step, the leaf was extracted with water using different solid to liquid ratios (1:10 w/v to 1:20 w/v) at 80ºC for 60 mins. The 1:10 leaf to water ratio yielded the highest AA (45.3% inhibition of DPPH) and TPC (83.6 mggae/g). Therefore, this ratio was chosen for subsequent experiments. Thereafter, the effects of different extraction temperatures (60ºC to 100ºC) and times (10 to 60 mins) on the extraction kinetics of AA, TPC, stevioside, and rebaudioside A were evaluated. The parameters in Peleg and Page models were calculated. Statistical tests (r 2 and RMSD) verified that the predicted values from both models were concordance with the experimental measures. The developed kinetic models could be potentially applied to design and optimize extraction process of antioxidant and sweetener from stevia leaf. 324 Science and Technology in the Disruptive Era

331 H_021_PF: SENSORY CHARACTERISTICS AND CONSUMER PREFERENCE MAPPING OF PORRIDGE Aratchaporn Deeprasert* and Suteera Vatthanakul Department of Food Science and Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand * Abstract: Porridge is a well-known food for breakfast and often eaten as a healthy food. To develop a healthy porridge that are rich in protein, minerals and vitamins. The brown rice and egg white are chosen as the main ingredient to develop a new porridge that high in nutrition. Firstly, study on the preference of 9 porridge [4 commercial porridge (A, B, C and D) and 5 laboratory-prepared porridge made with brown rice : egg white ratios (R:E = 100:0, 70:30, 50:50, 30:70 and 0:100)]. Balanced incompletely block design (BIB) was used for serve 9 porridge with 90 consumers. Analysis of variance showed that the laboratory-prepared porridge made with brown rice : egg white ratio 100:0 and 30:70 were liked most (p 0.05) and this was linked to the attribute of porridge (salty, flavor and viscosity). From preference mapping, the majority of consumers ranked taste as the most important characteristic influencing their purchase intent, followed by color of porridge and viscosity. Keywords : egg white, brown rice, porridge, preference mapping, partial least square (PLS) 325 The 44 th Congress on Science and Technology of Thailand (STT 44)

332 SESSION SP4: CRYSTALLOGRAPHY 326 Science and Technology in the Disruptive Era

333 SP4_001_PA: TWO NOVEL LANTHANIDE COORDINATION POLYMERS BASED ON CHLOROBENZHYDRAZIDE DERIVATIVES Nucharee Chongboriboon, Kittipong Chainok, * Materials and Textiles Technology, Faculty of Science and Technology, Thammasat University, Thailand * kc@tu.ac.th Abstract: Two new lanthanide coordination polymers, [Tb(2-Clbzz)(2-Clben) 2](NO 3) (1) and [Er(4-Clben) 3] (2), were synthesized by the reactions of lanthanide nitrate and 2-chlorobenzhydrazide (2-Clbzz) or 4-chlorobenzhydrazide (4-Clbzz) under hydrothermal conditions, and structural characterized by X-ray crystallography. Polymer 1 crystallizes in the orthorhombic space group Pccn and features a 1D chain structure in which the 2-chlorobenzoate (2-Clben) acts as bridging ligand to coordinate with Ln(III) ions, while the 2-Clbzz adopts only a bidentate chelating ligand through the carbonyl oxygen atom and one hydrazide nitrogen atom. Polymer 2 crystallizes in the triclinic space group P-1 and displays a 1D ribbon like chain structure constructed by the 4-Clben ligands are linked the adjacent Ln(III) ions in the bidentate-chelating and tridentate bridging-chelating modes. In both polymers, the chains are connected via intermolecular C H Cl, Cl Cl and π π interactions, forming a three-dimensional supramolecular structure. 327 The 44 th Congress on Science and Technology of Thailand (STT 44)

334 SP4_002_PA: SERIES OF ZINC(II) INTERPENETRATED COORDINATION POLYMERS BASED ON TRICARBOXYLATE AND DIFFERENT N-CONTAINING LIGANDS Kenika Khotchasanthong, 1 Winya Dungkaew, 2 Filip Kielar, 3 Karthik Chandrasekaran, 1 Kittipong Chainok 1, * 1 Materials and Textiles Technology, Faculty of Science and Technology, Thammasat University, Thailand 2 Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand 3 Department of Chemistry, Faculty of Science, Naresuan University, Thailand * kc@tu.ac.th Abstract: Four new luminescent zinc(ii) based coordination polymers containing mixed ligands, namely, [Zn 3(btb) 2(pz)] solvent (1), [Zn 2(btb) 2(Im) 2] solvent (2), [Zn(btb)(ppy)][(CH 3) 2NH 2] solvent (3) and [Zn 2(btb) 2(apm)][(CH 3) 2NH 2] 2 solvent (4), where apm = aminopyrimidine, btb = 1,3,5-Benzenetribenzoate, im = imidazole, pz = pyrazine, ppy = 4-phenylpyridine, were successfully synthesized under hydrothermal conditions and their structures were determined using single crystal X-ray diffraction. Compound 1 crystallizes in the orthorhombic with space group Pnma and exhibits two-fold interpenetrated three-dimensional network. Compounds 2 and 3 crystallize in the same monoclinic space group P2 1/n displaying a two-dimensional layer structure. Compound 4 crystallizes in the triclinic space group P-1 and exhibits a two-dimensional layer structure. Furthermore, all compounds show high thermal stability up to 400 C and exhibit fluorescent emissions in the solid state at room temperature. 328 Science and Technology in the Disruptive Era

335 SP4_003_PA: A NEW LUMINASCENT POROUS LANTHANIDE COORDINATION POLYMER FOR SELECTIVE SENSING OF SMALL ORGANIC MOLECULE AND METAL IONS Nutcha Ponjan 1, Winya Dungkeaw 2, Filip Kielar 3, Karthik Chandrasekaran 1, Kittipong Chainok 1 * 1 Materials Innovation and Technology, Faculty of Science and Technology, Thammasat University, Thailand 2 Department of Chemistry, Faculty of Science, Maharasakham University, Thailand 3 Department of Chemistry, Faculty of Science, Naresuan University, Thailand * kc@tu.ac.th Abstract: A new porous lanthanide coordination polymer containing oxalate (ox) linker, namely, [NaTb(OH)(ox) 1.5] 2H 2O, (1), was synthesized under solvothermal condition. Compound 1 crystallizes in the tetragonal system with I4/m space group and present a three-dimensional open framework consisting [Ln 4(μ 3-OH) 4] cluster units and Na(I) ions linked by bridging ox ligands. The emission spectrum in the solid state at room temperature of 1 exhibits characteristic bands of Tb 3+ with a strong green luminescence upon excitation under visible light. Furthermore, sensing properties of 1 for small organic molecules and metal ions were investigated and discussed in detail. 329 The 44 th Congress on Science and Technology of Thailand (STT 44)

336 SP4_004_PA: SINGLE CRYSTAL TO SINGLE CRYSTAL TRANSFORMATIONS IN COPPER- BIPYRIDINE-TEREPHALATE FRAMEWORKS Kodchakorn Samakun, 1 Siripak Jittirattanakun, 2 Winya Dungkeaw, 3 Karthik Chandrasekaran, 1 and Kittipong Chainoka 1 * 1 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Thailand 2 Division of Chemistry, Faculty of Science and Technology. Thammasat University, Thailand 3 Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand * kc@tu.ac.th Abstract: A novel three-dimensional copper(ii) coordination polymer containing 2,5- dibromoterephthalate (Br 2tp) and 4,4'-bipyridine (4,4'-bipy), [Cu(Br 2tp)(4,4'-bipy)](H 2Br 2tp) (1), was hydrothermally synthesized. This inclusion compound forms by the template molecule of H 2Br 2tp. When removing the template molecule by immersion in solvent produced a two-dimensional (4,4) square grid net of [Cu(Br 2tp)(4,4'-bipy)(H 2O)] (1'), showing single crystal to single crystal transformation. The solid-state structural transformation have been demonstrated and monitored by single-crystal X-ray diffraction, powder X-ray diffraction, and TG analysis studies. 330 Science and Technology in the Disruptive Era

337 SP4_005_OA: GAS ADSORPTION IN LANTHANIDE METAL-ORGANIC FRAMEWORKS BASED ON TETRABROMOTEREPHTHALIC ACID Suwadee Jiajaroen, 1 Winya Dungkaew, 2 Kanokwan Kongpatpanich, 3 Karthik Chandrasekaran, 4 Kittipong Chainok 4 * 1 Division of Chemistry, Faculty of Science and Technology, Thammasat University, Thailand 2 Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand 3 The school of Molecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology, Thailand 4 Materials and Textiles Technology, Faculty of Science and Technology, Thammasat University, Thailand * kc@tu.ac.th Abstract: Four novel 3D lanthanide metal-organic frameworks based on tetrabromoterephthalic acid (H 2Br 4tp) ligand of formula [Ln(Br 4tp) 0.5] H 2O (Ln = Er, Tm, Yb, Lu) have been synthesized by solvothermal technique and characterized by single-crystal X-ray diffraction. The structural analysis revealed that all title compounds crystallize in the trigonal space group P c1. These isostructural compounds exhibit a three-dimensional structure in which the octahedral Ln 3+ centers are bridged through the carboxylate groups of Br 4tp ligands. In the crystal structures, the frameworks are stabilized by hydrogen bonding and halogen-halogen interactions. The thermal stability and gas sorption properties of all compounds were investigated and discussed in detail. 331 The 44 th Congress on Science and Technology of Thailand (STT 44)

338 SP4_006_PA: LANTHANIDE COORDINATION POLYMERS WITH BENZOATE DERIVATIVES LIGAND: THERMAL AND OPTICAL PROPERTIES Chatphorn Theppitak, 1 Filip Kielar, 2 Kittipong Chainok 3, * 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani 12121, Thailand 2 Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand 3 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12121, Thailand * kc@tu.ac.th Abstract: Luminescent lanthanide coordination polymers composed of lanthanide ions and organic joint ligands exhibit characteristic photophysical and thermostable properties that are different from typical organic dyes. Various types of luminescent Eu(III) and Tb(III) coordination polymers, namely [Ln(Bz) 3(Bzz)(H 2O)] H 2O (1Ln; Ln = Pr, Nd, Sm, Eu, Gd, Tb, Dy, Er; Bz = benzoate; Bzz = benzhydrazide) and [Ln 3(Bz) 9] (2Ln; Ln = Eu, Tb, Dy, Er), were synthesized and characterized by single crystal X-ray diffraction. Compounds 1Ln crystallizes in a monoclinic space group P2 1/c and displays a 1D chain structure in which the Bz ligand acts as monodentate and bridging ligand to coordinate with Ln(III) ions. While the Bzz ligand adopts only a bidentate chelating ligand through the carbonyl oxygen atom and one hydrazide nitrogen atom. Each 1D chain is connected by the water of crystallization through intermolecular N H O and O H O hydrogen bonds to yield a 3D supramolecular network. Compounds 2Ln crystallizes in a monoclinic space group P2 1/n and present a 1D ribbon like chain structure constructed by the Bz ligands are linked to the adjacent Ln(III) ions in the bidentate-chelating and tridentate bridging-chelating modes. The ribbon chains interact via π π stacking interactions between adjacent Bz ligands, forming a 3D supramolecular framework architecture. 332 Science and Technology in the Disruptive Era

339 SP4_007_OA: SUPRAMOLECULAR AND HIRSHFELD SURFACE ANALYSES IN SCHIFF BASE COBALT(II) COMPLEXES Siripak Jittirattanakun, 1 Nanthawat Wannarit, 1 Winya Dungkaew, 2 Karthik Chandrasekaran, 3 Kittipong Chainok 3, * 1 Division of Chemistry, Faculty of Science and Technology, Thammasat University, Thailand 2 Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand 3 Materials and Textiles Technology, Faculty of Science and Technology, Thammasat University, Thailand * kc@tu.ac.th Abstract: A mononuclear cobalt(ii) complex with bidentate Schiff base ligand [Co(NCS) 2L 2] 2MeOH, (Co MeOH), (where L = N-(2'-pyridylmethylene)-2,3-dimethylaniline) was synthesized and characterized. The complex (Co MeOH) is responsive to external stimuli such as small organic solvents viz. methanol, ethanol, propanol, isopropanol and acetonitrile showing reversible single-crystal-to-single-crystal transformations. In-depth analyses on these soft crystalline materials reveal that the crystal structure stability is primarily due to interactions of weak intermolecular forces and not dependent on the size of guest solvent molecules. Figure 1. Packing Feature of Soft Co II Complexes 333 The 44 th Congress on Science and Technology of Thailand (STT 44)

340 SP4_008_PF: THE NOVEL COPPER(II) COMPLEX CONTAINING BY 2-HYDROXYBENZOIC ACID AND 2-AMINOPYRIMIDINE FOR GAS SENSOR APPLICATION Pornsan Lueangseephet, 1 Supakorn Boonyuen, 1, * Sudarat Thammatudto, 1 Kittipong Chainok, 2 Natthakorn Phadungsak, 2 Sumana Kladsomboon, 3 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathumthani, Thailand 2 Materials Innovation and Technology, Department of Physics, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand 3 Center for Research and Innovation, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand * chemistrytu@gmail.com Abstract: A new Cu(II) complex of the formula, {[Cu 2(2-OHbza) 4(apm)] 2(2-OHbza)} n (2-OHbza = 2-hydroxybenzoic acid, apm = 2-aminopyrimidine) was synthesized and characterized by IR spectroscopy, CHN elemental analysis, UV-Visible spectroscopy powder X-ray diffraction and single crystal X-ray diffraction. The complex crystallizs in the triclinic space group P-1 with unit cell parameters a = (4) Å, b = (4) Å, c = (4) Å, α = (10), β = (9), γ = (10). The Cu(II) ion adopts a distorted square-pyramidal geometry by four O atoms from different four 2-OHbza ligands and one N atom from apm ligand. Two neighboring Cu(II) atoms are bridged by four carboxylate groups of the OHbza ligands to from a paddle wheel [Cu 2(O 2C )] secondary building unit, which are further linked via the apm ligands to generate a one-dimensional chain. In the crystal, O H O hydrogen bond and interaction are presented. Moreover, this complex exhibits a good selectivity for alcohol vapor. 334 Science and Technology in the Disruptive Era

341 SP4_009_OA: X-RAY CRYSTALLOGRAPHY OF - GLUCOSIDASE FROM Weissella confusa BBK-1 Karan Wangpaiboon, Pasunee Laohawuttichai, Piamsook Pongsawasdi and Kuakarun Kursong, * Structural and Computational Biology Research Group, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand, * Kuakarun.k@chula.ac.th Abstract: The - glucosidase (EC ) from Weissella confusa BBK-1 (WcAG) was cloned and overexpressed in E. coli BL21 (DE3). The enzyme revealed strong transglycosylation towards maltotriose. To understand substrate specificity and transglycosylation mechanism via 3D structure, crystallisation of recombinant WcAG was carried out by vapor diffusion method. WcAG crystal was appeared within a week after crystallization. The best crystal diffracted to 1.8 Å at the synchrotron source (NSRRC, Taiwan) and belonged to space group P4122 with unit cell parameters a = , b = , c= , = 90, β = 90, γ = 90. Three-dimensional structure of WcAG was solved by molecular replacement method. WcAG contains three domains including the N-terminal domain ( +β -strand), the ( /β) 8-barrel central domain and the C-terminal domain (β -strand). 335 The 44 th Congress on Science and Technology of Thailand (STT 44)

342 SP4_010_PA: IMPROVEMENT OF PRODUCTION AND CRYSTALLIZATION OF RECOMBINANT CRUSTINPm1 FROM THE BLACK TIGER SHRIMP Penaeus monodon Thatcheewa Apichatayanon, 1,2 Anchalee Tassanakajon, 1 Kuakarun Krusong 1,2, * 1 Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Structural and Computational Biology Research Group, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand * Kuakarun.K@chula.ac.th Abstract: Crustins are the cationic cysteine-rich antimicrobial peptides (AMPs) that play an important role in innate immune responses of crustaceans. Crustins contain glycine-rich and cysteine-rich regions at the N-terminus that essential for antibacterial activities. The single whey acidic protein (WAP) domain at the C-terminus possesses conserved arrangement of eight cysteine residues to form four intramolecular disulfide bonds that tightly pack the structure as four-disulfide core (4-DSC). To understand the antimicrobial mechanism of crustinpm1, this study aims to crystallize the recombinant crustinpm1 from the black tiger shrimp Penaeus monodon. The recombinant crustinpm1 was expressed in Pichia pastoris strain KM71 under the methanol induction, then purified by Ni-column and Gel filtration chromatography. The purified protein was crystallized using vapour-diffusion method. Initial crystallization screening yielded single and thin rod-shaped crystals. The optimization and improvement of crystallization conditions are in progress. The X-ray diffraction and data collection will be further studied. 336 Science and Technology in the Disruptive Era

343 SP4_011_PA: SYNTHESIS AND STRUCTURAL CHARACTERIZATION OF THE COPPER (I) MIXED COMPLEXES WITH 5-METHYL 1,3,4 THIADIAZOLE 2-THIOL AND TRIPHENYLPHOSPHINE LIGANDS Khin Su Su Han, Nararak Leesakul, Saowanit Saithong Department of Chemistry, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand * Abstract: The complexes of copper(i) halides series (Cl, Br, I) with 5-methyl-1,3,4-thiadiazole-3- thiols (mtdzth) and triphenylphosphine (PPh 3) have been synthesized and two series of the complexes are obtained; namely, the binuclear mixed-ligand complexes; [Cu 2(mtdztH) 2(PPh 3) 2Cl 2](1), [Cu 2(mtdztH) 2(PPh 3) 2Br 2](2) and [Cu 2(mtdztH) 2(PPh 3) 2 I 2](3) and the mononuclear mixed ligand complexes [Cu(mtdztH)(PPh 3) 2Cl](4), [Cu(mtdztH)(PPh 3) 2Br](5) and [Cu(mtdztH)(PPh 3) 2I](6). All the complexes have been characterized by single-crystal X-ray diffraction. The Cu(I) atoms all complexes adopt a tetrahedral coordinated geometry. In the binuclear complexes, two adjacent Cu atoms are doubly bridged by the S atoms of the two mtdzth ligands, forming a Cu 2S 2 central core and each of which is coordinated by the P atom from the PPh 3 and a terminally bonded with halide atoms, Fig.1. In the mononuclear complexes, the Cu central atom is coordinated by one S atom of mtdzth, two P atoms of PPh 3 ligands and one halide atom (Cl, Br, I), Fig.2. In addition, the complexes have also been characterized by NMR, IR spectroscopy, and thermogravimetric analysis. Figure 1. The binuclear complexes (X=Cl, Br, I) Figure 2. The mononuclear complexes (X=Cl, Br, I) 337 The 44 th Congress on Science and Technology of Thailand (STT 44)

344 SP4_012_PA: SYNTHESIS, SPECTROSCOPIC CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF TWO NEW MONONUCLEAR TERNARY NICKEL(II) COMPLEXES Phichitra Phiokliang, 1 Supakorn Boonyeun, 1 Kittipong Chainok, 2 Pariya Na Nakorn, 3 Nanthawat Wannarit 1 * 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Khlong Luang, Pathum Thani 12121, Thailand 2 Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Khlong Luang, Pathum Thani 12121, Thailand 3 Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Klong Luang, Pathum Thani 12121, Thailand * nanthawat0110@gmail.com Abstract: Two new mononuclear ternary Ni(II) complexes forming from benzoate (benz) and chelating bipyridine derivatives, 2,2 -dipyridylamine (dpyam) and 1,10-phenanthroline (phen) namely, [Ni(dipyam)(benz) 2] (1) and [Ni(phen)(benz) 2][Ni(phen)(benz) 2(H 2O)] (2) were synthesized. These products have been characterized by elemental analysis, FT-IR, solid state diffuse reflectance spectroscopy and single-crystal X-ray diffraction method. Complex 1 crystallizes in the monoclinic space group P2 1/c and complex 2 crystallizes in the triclinic space group P. The structure of complex 1 presents a distorted octahedral geometry with [NiN 2O 4] chromophore (Fig. 1a). While, complex 2 presents two independent-monomeric units (Fig. 1b) with a distorted octahedral geometry. The antimicrobial activity of both complexes have been investigated by using paper disc diffusion method for Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The results show that complex 1 present a good antimicrobial activity Staphylococcus aureus, while complex 2 exhibits good antimicrobial activity for Escherichia coli. (a) (b) Figure 1. Structure of [Ni(dpyam)(benz) 2] (a) and [Ni(phen)(benz) 2][Ni(phen)(benz) 2(H 2O)] (b) 338 Science and Technology in the Disruptive Era

345 SP4_013_OA: TWO NOVEL CADMIUM(II) COORDINATION POLYMERS : SYNTHESIS, CHARACTERIZATION, CRYSTAL STRUCTURES AND PHOTOCATALYSIS PROPERTIES Ploy Assavajamroon 1, Pornchanok Pannoy 1, Kittipong Chainok 2, Sujittra Youngme 3 and Nanthawat Wannarit 1,* 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand, Department of Materials and Textiles Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand, Department of Chemistry, Faculty of Science and Technology, Khon Kaen University, Khon Kaen, Thailand, * nanthawat0110@gmail.com Abstract: Two novel cadmium(ii) coordination polymers [Cd(bzi) 2(Ag(CN) 2) 2] n (1) and [Cd(bix) 2 (Ag(CN) 2) 2] n (2) (bzi = 1-benzylimidazole and bix =1,4-bis(imidazol-1-ylmethyl)benzene) have been successfully synthesized, spectroscopically and structurally characterized. Compound 1 crystallizes in the monoclinic with C2/c space group, while compound 2 crystallizes in the orthorhombic with Pccn space group. The structure of both compounds presents interesting two-dimensional networks, which are 2-fold interpenetrating 2D networks for compound 1 and 2D undulating layers for compound 2. For 1, each Cd(II) centers are linked together by dicyanoargentate(i) to form 2D sheets extending in the bc plane and the sheets are stabilized by the argentophilic interactions. While, the metal centers in 2 are linked by the bix bridging ligand in trans configuration, generating the 2D sheets in the ab plane. The photocatalytic activity of both compounds for degradation of methylene blue (MB) in aqueous solution under UV irradiation has been examined. Figure 1. Coordination environment of {Cd(bzi) 2[Ag(CN) 2] 2} (1) (left) and {Cd(bix) 2[Ag(CN) 2] 2} (2) (right) 339 The 44 th Congress on Science and Technology of Thailand (STT 44)

346 SP4_014_PA: A LUMINESCENT ANIONIC METAL-ORGANIC FRAMEWORK FOR HIGHLY SELECTIVE Cu 2+ SENSING Natthakorn Phadungsak, 1 Supakorn Boonyuen, 2 Winya Dungkaew, 3 Filip Kielar, 4 Yan Zhou, 5 Karthik Chandrasekaran, 1 and Kittipong Chainok, 1, * 1 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Thailand 2 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Thailand 3 Department of Chemistry, Faculty of Science, Mahasarakham University, Thailand 4 Department of Chemistry, Faculty of Science, Naresuan University, Thailand 5 Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong * kc@tu.ac.th Abstract: A luminescent three-dimensional metal-organic framework, [BaZn 3(TMA) 3(H 2O) 3](ImH). 2H 2O (1), where TMA = trimesate, ImH = imidazolium, was hydrothermally synthesized and characterized. Structural analysis shows that compound 1 is a three-dimensional network with 1D channel representing a binodal 3,5- connected topology with a Schläfli symbol (3.9 2 ).( ). More importantly, 1 displays an intense luminescence in the solid state with a fluorescent emission peak at 410 nm. It also exhibits a high sensitivity and selectivity for Cu 2+ ion sensing in aqueous solution, even with very low concentration limits of about 12.8 μm. The possible sensing mechanism was also investigated by using XPS. Figure 1. View of 3D framework for [BaZn 3(TMA) 3(H 2O) 3], hydrogen atoms, ImH and H 2O molecules are omitted for clarity. 340 Science and Technology in the Disruptive Era

347 SP4_015_PA: A NOVEL THREE-DIMENTIONAL INTERPENETRATING COPPER(II) COORDINATION POLYMER CONSTRUCTING FROM 4,4 -BIPYRIDINE AND 2-HYDROXYBENZOATE LIGANDS Nanthawat Wannarit 1,* Thawanrat Saelim 1, Kittipong Chainok 2, Hsiung Chou 3 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand, Department of Materials and Textile technology, Faculty of Science and Technology, Thammasat University, Pathum Thani, Thailand, Department of Physics, National Sun Yat-Sen University, Kaohsiung Taiwan, The Republic of China, * nanthawat0110@gmail.com Abstract: A novel three-dimensional interpenetrating copper(ii) coordination polymers formulated as {[Cu(4,4 -bpy) 1.5(2-OHbenz)(NO 3)][Cu(4,4 -bpy)(2-ohbenz) 2]} n (I) (4,4 -bpy = 4,4 -bipyridine, 2-OHbenzH = 2-hydroxybenzoic acid) were prepared by direct method reaction of Cu(NO 3) 2 3H 2O, 4,4 -bpy and 2-OHbenzH with the mole ratio of 1:1:1. This compound was characterized by means of infrared spectroscopy, UV-Vis spectroscopy, TGA, powder and single crystal X-ray diffraction. For the X-ray structural analysis, compound I crystallizes in triclinic space group P-1. The molecular structure of this compound consists of two independent coordination networks, one-dimensional ladder chain-like structure [Cu(4,4 -bpy) 1.5(2- OHbenz)(NO 3)] n and the brick wall layer [Cu(4,4 -bpy)(2-ohbenz) 2] n. Interestingly, the interpenetration between these two independent coordination polymers provides a three-dimensional (1D+2D 3D) interprenetrating architecture. The crystal structure of this compound is stabilized by intermolecular hydrogen bonds, and C-H interactions. In addition, the magnetic properties of compound I presents paramagnetic behavior. The details of crystallographic data are shown in the poster. 341 The 44 th Congress on Science and Technology of Thailand (STT 44)

348 SP4_016_PA: NOVEL MOF LUMINESCENT PROBE FOR EFFICIENT SENSING OF COPPER(II) AND IRON(III) IONS Kittipong Chainok* Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12121, Thailand * Abstract: The combination of the intrinsic luminescent features of d 10 metal ions or lanthanide ions and π-electron-rich ligands together with the unique characteristics of MOFs provides a fascinating opportunity for designing novel porous MOF-based sensors. In this work, two highly luminescent porous MOF materials, namely, [BaZn 3(TMA) 3(H 2O) 3](ImH) 2H 2O (1) and [NaTb(μ-OH)(ox) 1.5] 2H 2O (2), where TMA = trimesate, ImH = imidazolium, ox = oxalate, were successfully synthesized. All MOFs feature a three-dimensional framework structure. 1 and 2 exhibit a high sensitivity and selectivity for Cu 2+ and Fe 3+ ions, respectively. 342 Science and Technology in the Disruptive Era

349 SP4_017_PA: SYNTHESIS, CHARACTERIZATIONS AND X-RAY STRUCTURAL STUDIES OF NOVEL ONE-DIMENSIONAL LADDER-LIKE STRUCTURE COBALT(II) COORDINATION POLYMER Phakamat Promwit, 1 Kittipong Chainok, 2 Sujittra Youngme, 3 Nanthawat Wannarit 1,* 1 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Khlong Luang, Pathum Thani, Thailand, Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Khlong Luang, Pathum Thani, Thailand, Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, * nanthawat0110@gmail.com Abstract: A novel one-dimensional ladder-like structure Co(II) coordination polymer, {[Co 2(4,4'-bpy) 2(3-Clbenz) 3(H 2O)] CH 3OH} n (I) (3-ClbenzH = 3-chlorobenzoic acid and 4,4 -bpy = 4,4 -bipyridine) was synthesized and characterized by IR spectroscopy, diffuse reflectance spectroscopy, thermogravimetric analysis (TGA) and single crystal X-ray diffraction. The crystal structure of Co(II) complex crystallizes in the triclinic system, P-1 space group. Compound I consists of two crystallographically independent Co(II) centers, which adopt a distorted octahedral geometry with different coordination environments, [CoN 3O 3] and [CoN 2O 4] for Co(1) and Co(2) chromophores, respectively. Each Co(1) center is coordinated by two terminal 3-Clbenz ligands and connected together by 4,4 -bpy bridging ligands, providing one-dimensional linear chain-like structure along a axis. These two adjacent chains are linked by [Co(2)(4,4'-bpy) 2(3-Clbenz) 2(H 2O) 2] units via two 4,4 -bpy bridging ligands, leading to interesting one-dimensional ladder-like structure. The crystal structure is stabilized by mainly intermolecular stacking and hydrogen bonding interactions. Figure 1. Molecular structure of {[Co 2(4,4'-bpy) 2(3-Clbenz) 3(H 2O)] CH 3OH} n (I), methanol molecules and hydrogen atoms are omitted for clarity. 343 The 44 th Congress on Science and Technology of Thailand (STT 44)

350 SP4_018_PA: TWO NEW SILVER(I) COORDINATION POLYMERS BASED ON FLEXIBLE SCHIFF BASE LIGANDS Aiyada Rungruang, 1 Thapong Teerawatananond, 1 Siripak Jittirattanakun, 2 Kittipong Chainok 3, * 1 Division of Chemistry, Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under the Royal Patronage, Thailand 2 Division of Chemistry, Faculty of Science and Technology, Thammasat University, Thailand 3 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Thailand * kc@tu.ac.th Abstract: Self-assembly reactions of silver(i) nitrate with the newly flexible Schiff based ligands, i.e. 1,4-phenylenedimenthylylidene-bis-4-chloroaniline (PB-ClA) or 1,4-phenylenedimenthylylidene-bis-4-chloroaniline (PB-BrA) in a mixture solution of MeOH and CH 2Cl 2 afforded an isostructural of two new silver(i) coordination polymers with the general formula [Ag 3(PB-XA) 2(NO 3) 3], X = Cl (1), Br (2). The compounds crystallize in the centrosymmetric triclinic space group P-1 with three independent Ag(I) atoms in the asymmetric unit. The Ag(I) ions are linked by the PB-XA ligands and NO 3 molecules to form a one-dimensional chain along the crystallographic b axis with the Ag Ag separations lie in the range Å. These chains are further linked into a three-dimensional network via π π stacking and weak C H X halogen bonds. 344 Science and Technology in the Disruptive Era

351 SP4_019_PA: CRYSTAL STRUCTURE AND HIRSHFELD SURFACE ANALYSIS OF 4,4'-(5,5'- DISULFANEDIYLBIS(TETRAZOLE-5,1-DIYL))DIBENZOIC ACID Napatsorn Sawengsiriphon, 1 Suwadee Jijaroen, 2 Kittipong Chainok 1, * 1 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12121, Thailand 2 Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani 12121, Thailand * kc@tu.ac.th Abstract: A new organic compound containing dicaroxylic acid and disulfide groups, C 16H 10N 8O 4S 2, (systematic name: 4,4'-(5,5'-disulfanediylbis(tetrazole-5,1-diyl))dibenzoic acid) was prepared and structural characterized by single crystal X-ray diffraction. The compound crystallizes in in the orthorhombic space group Fdd2. In the crystal, the molecules are linked by strong N H O and O H O hydrogen bonds along with aromatic π π stacking, completing a three-dimensional supramolecular structure. The intermolecular interactions in the crystal packing were further analysed using Hirshfield surface analysis. 345 The 44 th Congress on Science and Technology of Thailand (STT 44)

352 SP4_020_PA: A SERIES OF TWO-DIMENSIONAL LANTHANIDE COORDINATION POLYMERS BASED ON THE CHLORANILIC ACID Akkawat Udompipat, Kenika Khotchasanthong, Kittipong Chainok* Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Thailand * kc@tu.ac.th Abstract: A series of self-assembled two-dimensional lanthanide coordination polymers (LnCPs) involving the chloranilic acid (H 2ca) ligand [Ln(ca) 1.5(H 2O) 3] 4H 2O (Ln = Sm, Eu, Gd, Tb, Dy, Er, Tm, Yb) has been hydrothermally synthesized and characterized by elemental analysis, IR spectroscopy, X-ray single-crystal diffraction, powder X-ray diffraction, thermogravimetry and photoluminescence spectroscopy. These compounds are isostructural and crystallize in the triclinic P-1 space group with one Ln 3+ ion, three half-occupied ca 2 moieties, three coordinated water molecules, and three and the one of half-occupied uncoordinated water molecules in the asymmetric unit. In the crystal, each ca 2 ligands are bridged two Ln 3+ ions via oxygen atoms, resulting in the formation of a two-dimensional (2D) layer. These 2D layers are packed together to form 3D framework structures through the classical O H O hydrogen bonds involving coordinated water molecules and isolated water molecules. Solid state photoluminescence of compounds Eu and Tb were investigated at room temperature. 346 Science and Technology in the Disruptive Era

353 SP4_021_PA: A NOVEL ZINC(II) COORDINATION POLYMER (ZN(II)-CP) BASED ON MIXED- LIGANDS AS FE(III) SENSOR Jiraporn Buasakun, 1 Kittipong Chainok, 2 Tanwawan Duangthongyou 1,* 1 Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand 2 Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, PathumThani 12120, Thailand * fscitwd@ku.ac.th Abstract: A new Zinc(II) coordination polymer based on mixed-ligand (citrate (citric acid) and 1,2-di(4-pyridyl)ethylene (dpe) as O-donor and N-donor, respectively.) namely, [Zn 2(citrate)(formate)(H 2O)(dpe)].2H 2O was synthesized by hydrothermal method. The crystal structure was analyzed by single crystal x-ray diffraction and it showed the coordination sphere around Zn 2+ is trigonal bipyramidal. The interesting citrate ligand acts as bridging ligand between two Zn 2+ by linked together with two carboxylate groups at terminal of citrate providing 1D chain. The carboxylate group on the carbon at the middle also bridge between two Zn 2+ in neighbour chain resulting double chains. Each double chains is further link together by - stacking between dpe ligand forming 2D layer. The structure was also confirmed by CHN elemental analysis, FTIR, and thermogravimetric analysis. The fluorescence property of compound was studied and show emission band at 369 nm ( ex = 280 nm). The compound was used as cation sensor which exhibit selective to Fe 3+ ion. Figure 1. The 2D layer of compound generated by - stacking of dpe ligand (for clarity, solvent water molecules and all hydrogen atoms are omitted) 347 The 44 th Congress on Science and Technology of Thailand (STT 44)

354 SP4_022_PA: SYNTHESIS AND PROPERTIES OF A NEW ADENINE-BASED Zn (II) COORDINATION POLYMERS FOR SENSITIVE SENSING OF NITROAROMATICS. Phakinee Srilaoong, 1 Kittipong Chainok, 2 and Tanwawan Duangthongyou 1 * 1 Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand 2 Materials Innovation and Technology, Department of Physics, Faculty of Science and Technology, Thammasat University, PathumThani 12120, Thailand * address: fscitwd@ku.ac.th Abstract: The hydrothermal reaction between adenine (ad) and biphenyl-3,3,5,5- tetracarboxylic acid (BPTC) organic lingers with Zn(II) ions has been prepared and the complex (1) namely [Zn 4(ad) 3(BPTC)(H 2O) 3] H 2O was obtained. Single crystal X-ray diffraction reveals that complex (1) possesses three different coordination sphere around Zn (II) ions centers, tetrahedron (ZnN 3O), distorted tetrahedron (ZnN 2O 2) and octahedron (ZnNO 5). The adenine and BPTC ligands act as bridging ligand with coordination modes µ 3, ŋ 1 : ŋ 1 : ŋ 1 : and µ 4, ŋ 1 : ŋ 2 : ŋ 1 : ŋ 2 : respectively. The 3D framework was formed from the various coordination modes of the ligands. The complex (1) was structural characterized by X-ray diffraction powder, FT-IR, UV-visible diffuse reflectance, Thermogravimetric analysis and elemental analysis. Solid-state photoluminescence of complex (1) was studied. Which shows maximum emission band at 345 nm ( ex = 310). In addition, complex (1) acts as sensor for detection of nitroaromatics compound via quenching of its fluorescent intensity. 348 Science and Technology in the Disruptive Era

355 349 The 44 th Congress on Science and Technology of Thailand (STT 44)

356 350 The 44 th Congress on Science and Technology of Thailand (STT 44)

357 351 The 44 th Congress on Science and Technology of Thailand (STT 44)

358 352 The 44 th Congress on Science and Technology of Thailand (STT 44)

359 353 The 44 th Congress on Science and Technology of Thailand (STT 44)

360 354 The 44 th Congress on Science and Technology of Thailand (STT 44)

361 355 The 44 th Congress on Science and Technology of Thailand (STT 44)

362 356 The 44 th Congress on Science and Technology of Thailand (STT 44)

363 357 The 44 th Congress on Science and Technology of Thailand (STT 44)

364 ACKNOWLEDGEMENT FOR THE SPONSORS OF STT44 DIAMOND TPI POLENE PUBLIC COMPANY LIMITED NATIONAL SCIENCE MUSEUM ASIAN PHYTOCEUTICALS PUBLIC COMPANY LIMITED TITANIUM BANGKOK BANK PUBLIC COMPANY LIMITED BARA SCIENTIFIC COMPANY LIMITED GOLD NATIONAL SCIENCE TECHNOLOGY AND INNOVATION POLICY OFFICE POWER OF INNOVATION FOUNDATION NATIONAL INNOVATION AGENCY 358 Science and Technology in the Disruptive Era

365 SILVER THE THAILAND RESEARCH FUND THE MINISTRY OF SCIENCE AND TECHNOLOGY OF THAILAND NICKEL FOUNDATION FOR THE PROMOTION OF SCIENCE AND TECHNOLOGY UNDER THE PATRONAGE OF HIS MAJESTY THE KING THE SIAM CEMENT GROUP PUBLIC COMPANY LIMITED BRONZE THAI UNION GROUP PUBLIC COMPANY LIMITED SYNCHROTRON LIGHT RESEARCH INSTITUTE SERVE SCIENCE COMPANY LIMITED SCILUTION COMPANY LIMITED ISCIENCE TECHNOLOGY COMPANY LIMITED 359 The 44 th Congress on Science and Technology of Thailand (STT 44)

366 EXHIBITION BOOTHS BECTHAI BANGKOK EQUIPMENT & CHEMICAL COMPANY LIMITED UNIVERSAL SCIENCE LIMITED PARTNERSHIP WORATHAN TECHNOLOGY COMPANY LIMITED CESCO LIMITED MERCANTILE HI-TECH COMPANY LIMITED BIO-RAD LABORATORIES LIMITED BIO-ACTIVE COMPANY LIMITED CTi & SCIENCECOMPANY LIMITED 360 Science and Technology in the Disruptive Era

367 361 The 44 th Congress on Science and Technology of Thailand (STT 44)

368 Author Index Abdulhakim Masa Abdulnaser Hajisamoh Aiyada Rungruang Akkaneewut Chabangborn Akkawat Udompipat Akkharat Sangaroon Alesa Tohbaru Amornrat Promboon Ampira Charoensaeng Anan Sirithanyarat Anchalee Junkaewb Anchalee Tassanakajon Anchana Prathep Ancharida Savarajara , 173 Aniwat Kesorn Anjalee Vacharaksa Anumart Buakeaw , 108, 109 Anupong Joompang Anuttara Udomprasert Anwaraporn Suramitr Aparporn Sakulkalavek Aphichart Karnchanatat Apichart Limpichaipanit Apilak Worachartcheewan Apinun limmongkon Apisit Prokaew Apitsara Charoenphon Aratchaporn Deeprasert Areerat Kunanopparat Ariya Dejtaradol Arnon Chukamnerd , 164 Arunee Karaket Asawin Sinsarp Ashman Adair Asma Longkunan Assuma Sainakum Atchara Maddolah Atchariya Leeyiamsakul Athipong Ngamjarurojana Atthaboon Watthammawut Aunsaya Eksatit Bancha Lawang Banchob Sripa , 190 Bandhita Saibandith Benjamaporn Jutapad Boonchoy Soontornworajit , 259 Boonpob Nowichai Boonsita Tongtokit Science and Technology in the Disruptive Era Boonyarit Kukaew Boonyisa Suksomboon Brian O. Patrick Bui Viet Hung Bungon Wannalux Busarakorn Mahayothee Butsarin Thaweekoon Chadapa Sakunwattana Chainarong Ruangthong , 208 Chaisingh Poo-Rakkiat Chaiwat Boonpeng Chaiya Prasittichai Chakraphan Sutthirat Chakrit Tachaapaikoon , 169, 318 Chaksawat Sangawitayakorn Chalermpong Saenjum Chalermporn Ongvarrasopone Chalida Haruehansapong Chan Dara Chanida Wootthichairangsan Chanitda Taweechat Chanitnart Phaothong Chanoknan Hattapanichaporn Chanokporn Muangchinda Chantaka Wongdontri Chantipa Lerswipapat Charernmee Chamchoy , 198, 201, 203, 205, 207 Charunee Meechoui Chatchai Muanprasat Chatphorn Theppitak Cheewarat Printrakoon Cheewita Suwanchawalit...214, 225, 268, 269, 271 Chetsada Pothiratana Chiraporn Ananchaipattana Chirat Sirimuangmoon Chockchai Theerakulkait Chodchanok Attaphong Cholapat Varongchayakul Cholaphan Deeleepojananan Chomchai Suksai Chomphunut Wiriyawatthana Christoph Sontag Christopher Richardson Churapa Teerapatsakul Chutha Takahashi Yupanqui Chutima Sriviboon Chutima Tanapichatsakul Damratsamon Surangkul

369 Decha Dechtrirat Dheerawan Boonyawan Dinh Phuoc Nguyen Duangamol Nuntasri Tungasmita Duanggamon Muengsaen Duangkamon Jiraroj Duangtip Sudhan Dujreutai Pongkao Kashima Duncan R Smith Duy Nguyen Vu Eakkasit Punrat Ekawan Luepromchai Fateehah Bueto Felipe Monteiro Gomes de Mattos Filip Kielar , 329, 332, 340 Foyfon Sensong Gholamreza Khaksar Gil Moretto Gitsada Panumonwatee Gulsiri Senawong Hafeeza Sakor Hnin Htet Kyaw Hsiung Chou Ind Tungsatitchai Ingorn Kimkong Intira Nualkham Irb Kheoruenromme , 127, 128 Irb Kheoruenromne , 123, 124, 126, 129 Isaraporn Polbuppha Iyiola Oluwakemi Owolabi Jakkapan Sirijaraensre Janjira Panchompoo Jaroon Jakmunee , 213, 215 Jaroon Junsomboon , 213, 215 Jaturavit Nimnuan Jean-Fabien Capsal Jeff Kuhn Jennarong Lungthaisong Jessada Wong-On Jiaranai Peantum Jirabhorn Piluk Jiraporn Buasakun Jitnapa Sirirak Jitwadee Wiangngean Jonathan Webb Jongkolnee Yaowapaksophon Jongrak Attarat Jureerut Daduang Juthamart Putthayakool , 205, 207 Juthaporn Paothong Kaew Udomsirichakorn Kamaruddin Waesateh Kanchalee Jongrangwitaya Kanchana Rotcharoen Kanchana Watla-iad , 220 Kangsadan Boonprab Kanlaya Prapainop Kannika Boonkue Kannika pratoompuk Kanoknan Khongsukwiwat Kanok-on Rayanil Kanokporn Pakeechai Kanokporn Triwitayakorn Kanokwan Kongpatpanich Kanphirom Lertbumroongchai Kansri Boonpargob Kansri Boonpragob Kanyarat Kwansirikul Karan Wangpaiboon Karn Serivalsatit Karthik Chandrasekaran , 329, 330, 331, 333, 340 Kawinnart Buaruang Kawinnat Buaruang Kenika Khotchasanthong , 346 Kessaya Vorapiboonvit Khamphe Phongthong Khamphe Phoungthong Khanok Ratanakhanokchai , 169, 318 Khin Su Su Han Kittibandid Soisuwan Kittikhun Wangkanont Kittinan Komolpis , 108, 109 Kittipong Chainok 327, 328, 329, 331, 332, 334, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348 Kittipong Chainoka Kittisak Chawawisit Kittiya Islam , 192 Kittiya Tantisuwanichkul Kodchakorn Samakun Korakot Chansareewittaya Kornlawat Tantivit Kritsadi Thetpraphi Kritsanu Tivakornsasithorn Krittika Dummunee Kuaanan Techato Kuakarun Krusong Kuakarun Kursong Kumari Dahama The 44 th Congress on Science and Technology of Thailand (STT 44)

370 Kunlanun Rungnarai Kunlaya Somboonwiwat , 151, 182, 184 Kwanjira Phanplado Laemthong Chuenchom , 278 Lalida Chanchaem Laongdow Jungrak , 201, 208 Lerluck Chitradon Likit Preeyanon Lionel Petit Makamas Sutthacheep. 197, 198, 199, 200, 201, 203, 205, 207, 208 Manassawee Kuhawijit Marisa De Bels Martin Hemberg Mary Sriwisade Mathinee Yoocharoen Mati Horprathum May Soe Thu May Soe Thwe May Thet Paing Phoo , 164 Minh Quyen Le Mongkol Phaengphech Mongkol Sukwattanasinitt Monsicha Somrit Monthon Lertcanawanichakul Montira Leelakriangsak Montree Hankoy Montri Aiempanakit , 269 Muhammad Iqbal Perdana Muttakeen Che-leah Nabil Hayeemasae Nadia Doloh Nalena Praphairaksit , 106 Nanthawat Wannarit , 338, 339, 341, 343 Napaporn Roamcharern Napat Suebsiri Napatsorn Sawengsiriphon Naphatsanan Somngam Nararak Leesakul Narin Charoenphun Narisorn Kitiyanant Narong Arunrut Natsarun Saichana Natta Laohakunjit Nattawat Chatphuak Nattaya Srisawad Natthakan Rungraeng Natthakorn Phadungsak , 340 Natthakorn Uppatam Natthawut Yodsuwan Science and Technology in the Disruptive Era Natthaya Thepphanao Natthiya Saehlim Nattiya Hirankarn Nawan Pornananrat Nawee Kungwana Ngamnit Wongcharoen Nhung Ngo Thi Tuyet Nianrawan Meecharoen Nichaphat Kanoksinwuttipong Nicholas Keone Lee Nilobon Komonsing Nipada Ruankaew Disyatat Nipitpon Kongjan Nisachon Jangpromma.. 112, 113, 134, 138, 140, 155, 156 Nisanart Charoenlap , 168 Nitchakarn Sumsiripong Nithiwat Suntichaikamolkul , 149 Nitsupa Luksum Niva Sthapit Nonnanat Yooyuen Nootcharin Wasukan Nopchonsorn Manpiankit Noppadon Nuntawong Nopporn Jongkamonwiwat Noulkamol Arpornpong , 285 Nucharee Chongboriboon Numpon Insin Nunticha Somrit Nustsaba Kronburee Nut Phueakphud Nutcha Ponjan Nuttavit Kumvijairat Nuttha Thongchul Nutthachai Lertamornthum Nuttharuja Hirunburana Obnithi Noppha Onpawee Sangsai Onruthai Pinyakong Ontima Yamchuti Orapan Romruen , 320, 321 Orathep Mue-suea Orawon Chailapakul Orrapa Cheycharoen Pacharee Kaewchana Paiboon Vattanaviboon , 168 Paitoon Rashatasakhon Pajaree Thongsanit Pajareeya Songserm Pakathip Thakaeng

371 Pakorn Lekvongphiboon Pakpoom Boonchuen Panadda Tansupo Panida Watchanung Panisa Aimvijarn Pannipha Dokmaingam Panyawut Rattanarom Paphada Watcharapo Paphitchaya Khomweerawong Parichart Yamalee Parichat Niyomthai...198, 201, 203 Parichat Thepthong Parichut Thummarati Pariya Na Nakorn Pasawut Kokilakanistha Pasin Rattanasin Pasunee Laohawuttichai Patamaporn Umnahanant Patchara Mongkolsuk Patcharamporn Khamchana Patcharee Pripdeevech , 118, 314 Patcharin Saeng-in Patchariya Chawalitjinda Patimaporn Wongprompitak Patompon Wongtrakoongate Pattama Ekpo Pattanawit Swanglap Pattapong Kessomboon , 190 Pattara Thiraphibundet , 255 Pattariya Singpant Patthamawadee Tongkaew Patthra Pason , 169, 318 Pattiya Pibulchinda Pavisuth Kanjantra Pawaphat Jaturaphagorn Pechpikul Khumon Peerapong Wongpituk Penchit Chitnumsub Penjai Sompongchaiyakul , 287 Penphitcha Amonpattarakit Phaijit Sritananuwat Phakamat Promwit Phakinee Srilaoong Phanee Pidetcha Phatcharee Roekngandee Phattarunda Jaree , 184 Phichanan Duchda Phichitra Phiokliang Phimpisa Phraphuchamnong Piamsook Pongsawasdi , 335 Piboon Pantu Pichaiwat Chanalardpaisarn Pichit Olanwongsakul Pierre-Jean Cottinet Pimchai Chaiyen , 250 Pimchanok Phatho Pimonpan Kaewprachu Pimsiree Suwanna Pinate Ruenchan Pinyapat Jitphongsaikul Pipat Khongpracha Pirawish Limilawan Pirom Chenprakhon Pisit Dhamvithee Pisit Tangkijvanich Pisit Ubonsri Pitak Chuawong Pitakchai Fuangkeaw Pitchayathida Mee-udorn Pitsanupong Kanjanapayont Piyada Jittangprasert Piyanoot Uklam Piyanuch Wonganan , 235 Piyaphong Chenrai , 283 Piyarat Govitrapong Plaipol Dedvisitsakul , 220 Ploy Assavajamroon Ponlachart Chotikarn Poonyawee Keattanong Pornchanok Pannoy Pornpat samang Pornphimol Meesakul Pornsan Lueangseephet Prachak Inkaew Prachwanee Pibumrung Prakaymars Panumars Pramote Khuwijitjaru , 316 Pranisa Emyoo Prapaporn Nakarat Prapin Wilairat Prasan Swatsitang Prasong Kessaratikoon Prasong Khaenam Prawit Oangkhana Preeda Nathawat Preeyanit Buapeth Preeyaporn Phonalwut Prichukorn Khongsatra Primpisa Charoenprateepkit Pukchunya Siriwan The 44 th Congress on Science and Technology of Thailand (STT 44)

372 Punlop Tonapo Rachsak Sakdanuphab Ratanawadee Niemsiri Ratchadaporn Kueasook Ratikorn Vongkhae Ratree Wongpanya , 139 Rattanaruji Pomwised , 164, 167 Rattanavinan Hanchaina Rattanawadee Niamsiri Rattikan Chantiwas Rattiya Padungpol Rattiya Waeonukul , 169, 318 Raymond J. Andersen Rita Hairani Rojana Pornprasertsuk Ruchirus Muthikul Ruedee Jaratrotkamjorn Ruengwit Sawangkeaw , 115 Rungnapha Saeeng Rungsima Daroonpunt , 172 Rutchapon Hunkao Ruthairat Boonkrongcheep Ruttayaporn Ngasaman Ryo Maezono Sachiko Isobe Sajee Noitang , 115 Sakol Panyim Santad Wichienchot Santi Phosri Saovanee Boonrod Saovaros Svasti Saowanee Lertworasirikul Saowanit Saithong Saowapa Planonth , 215 Sarintip Sooksai , 115 Saroat Rawdkuen , 320, 321 Sarote Boonseng Sarun Keithmaleesatti Sarunpron Khreungsai Sarunyou Chusri , 167 Sasithon Khunthong Sasithorn Boonnoon Sathit Prasomphan Sira Sansuk Sirichai Khoukitpaisal Siriluck Rongprakhon Siriluck Anunnatsiri Sirinee Thiangthae Sirinit Tharntada Sirinthip Sosiangdi Science and Technology in the Disruptive Era Siripak Jittirattanakun , 333, 344 Siripan Kerdsut Sirirat Rengpipat , 109 Siriwat Shiraarnon Sitanan Thitiprasert Sitang Kongkratoke , 291 Sitthipong Polthum Sittipong Amnuaypanich Sittiporn Pengsakun , 200, 205, 208 Siwaporn Meejoo Smith Siwat Ruangroengkulrith Skorn Mongkolsuk , 168 Somboon Tanasupawat , 172, 173 Somchai Annusontpornpern Somchai Anusontpornperm 123, 124, 125, 126, 127, 128, 129 Somchart Maenpuen Sompong Klaynhongsruang Sompong Klaynongsruang.. 113, 134, 138, 140, 155, 156 Somprat Srithagon Somwan Chumphongphan Sonam Wangchuk Songchan Puthong , 108, 109 Songwut Suramitr Soontorn Suvokhiaw Sornkanok Vimolmangkang Souksady Insixiengmay Sreyneang Nhim Srisawad Stephen G. Pyne , 231 Suchada Chuanuwatanakul Sucharat Sanongkiet , 277 Sucharat Suksai Suchawadee Insawang Sudarat Thammatudto Sudatip Ruttanawijit Sujin Suwanna Sujittra Srisung Sujittra Youngme , 343 Sumana Kladsomboon Sunita Chamyuang Supaart Sirikantaramas , 145, 149 Supachai Vuttipongchaikij Supajit Sraphet Supakorn Boonyeun Supakorn Boonyuen , 334, 340 Supalax Srijaranai Supalurk Yiamsombut Supanut Pairohakul

373 Supaphorn Thumsorn Suparerk Borwornpinyo Supatra Porasupatana Supawadee Namuangruk Supawadee Somkane Supeeraya Arsa Suphakorn Katib Suphattha Kaeomani Suphicha Thanachit 122, 123, 124, 125, 126, 127, 128, 129 Suphot Musiri Supichaya Laothong Supitcha Wanvimonsuk Supitchaya Laothong Supot Hannongbua , 250 Suppakarn Chansareewittaya Suppalak Brameld Suppanat Supanyo Supphakarn Phoaduang Surachai Maijaroen Surachai Thachepan Suraphol Chunhabundit Surapon Piboonpocanun Surat Laphookhieo , 231, 240, 241 Suratsawadee Tiangpook Surinya Traipop Sutas Suttiprapa , 190 Suteera Vatthanakul Suteera Vatthanakul Sutha Khaodhiar Suthipapun Tumhom Sutinee Girdthep Sutinee Sinutok Sutthida Boomsamran Sutthida Boonsamran Suvimol Soithongsuk Suwadee Jiajaroen Suwadee Jijaroen Suwit Kiravittaya Tanan Chub-uppakarn Tanatorn Khotavivattana Tanwawan Duangthongyou Tanwawan Duangthongyou Tanyaporn Tiaotrakoon Taro Kawai Tassanee Phermthai Tavan Janvilisri Tawatchai Apidechkul Teeradon Kardudom Teerapong Prickchoopon Teeravisit Laohapensaeng Thamasak Yeemin. 197, 198, 199, 200, 201, 203, 205, 206, 207, 208 Thamrongjet Puttamuk Thanachan Mahawanich Thanaphon Jenwitheesuk Thanaporn Wichai Thanaset Senawong Thanatchaporn Tabtimsri Thanawan Srilert Thaned Kangsamaksin Thaneeya Hawiset Thanthip Tathong Thanyada Rungrotmongkol , 250 Thanyanuch Kriangkripipat Thapong Teerawatananond Thatcheewa Apichatayanon Thavorn Supaprom Thawalrat Ratanadachanakin Thawanrat Saelim Thaweesak Tana Theetawat Sriwichaikaew Thewarach Laha Thitinai Gaewdang Thitiphong Khamkhenshorngphanuch Thitipone Suwunwong Tichakorn Singto Tidapa Rattanaumpa Tinnakorn Saelee Tipapon Wongsooka Tirayut Vilaivan Titapa Tanawansombat Titaporn Tumpanuvat Titti Laojaruwat Todsapol Kornsri Torsak Luanphaisarnnont Truc Phan Thi Hong Tuangporn Suthiphongchai , 152, 192 Tulyapruek Tawonsawatruk Tumnoon Charaslertrangsi Tunyalux Huangsuwannakorn Udom Robkob Udomsak Palee Umaporn Pimpitak Umaporn Pimpitak , 108 Unchittha Prasatsap Usa Jeenjenkit Vanida lumpol Varaporn Tanrattanakul Varodom Charoensawan The 44 th Congress on Science and Technology of Thailand (STT 44)

374 Vasana Tolieng Vatcharin Suwannit Veerakit Vanitshavit Virayu Suthiphasilp Vongsathorn Ngampuak , 169 Wachirawit Chinantuya Wanchai De-Eknamkul Wanida Wonsawat Wanlaya Klinthong , 205 Wanna Sirisangtragul Wannarat Phonphoem Wannisa Arthan Wansika Kiatpathomchai Wantana Klysuban Wanwisa Kansa Warinthorn Chavasiri , 235 Waris Nuamnim Waruntorn Kanitpanyacharoen Warut Thammawichai Wasana Phantewee Watchara Samsuvan , 199, 200, 205, 208 Watcharee Saisongkorh Weena Siangproh Weerachet Jittanit Weerapat Surasaeng Weradaj Sukaead Weradej Sukaead Wetchasart Polyiam Wichin Suebpala , 207, 208 Wilaiwan Supap Wilawan Mahabusarakam Willard E. Collier Winya Dungkaew...328, 331, 333, 340 Winya Dungkeaw , 330 Wiparat Hotarat Wiphawan Aunkhongthong 198, 201, 203, 205, 207 Wisanu Maneerat , 241 Wisarut Payoungkiattikun Wiwit Tantibhedhyangkul Wongsakorn Phongsopitanun Wongwiwat Tassaneeyakul Wonnop Vissessanguan Woranon Leevongcharoen Worasak Sukkabot Worawan Bhanthumnavin Worawut Sevegoon Worrathon Tilokkarn Wuttichai Jaidee Yan Zhou Yanagi Tomohiro Yin May Tun Yingyod Lapwong Yongyut Manjit Yosapong Temsiripong Yotsakorn Tantiapibalkun Yupakanit Puangwerakul Science and Technology in the Disruptive Era

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