Artificial Insemination

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Jemima Washington
8 years ago
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1 Dr. Anil Deshpande LDO, Frozen Semen Laboratory, Kirkee, Pune- 3

2 Deposition of Semen in Genital Tract of female in heat by mechanical means Advantages of AI: Increases usefulness of Superior Sires Low cost Semen Transportation even beyond continents Avoids STDs

3 Limitations of AI Training & Special Equipments Inadequate skills, inputs, hygiene low fertility Semen Transportation requires intense care Extension education Breeding Policy

4 Signs of Heat Restless Urinates frequently Vulval lips Swollen and VMM bright red & moist Crystal clear ropy discharge Drop in Milk Production Bellowing optional Initial stage Mounting Standing Heat

5 Stages of Heat

6 Time Cow coming in heat 6a.m-noon 22% Noon-6p.m. 10% 6 p.m.-mid night 25% Mid night-6 a.m. 43%

7 Early heat in Buffalo, upper 1/3rd mucosa is pink

8 functions of cervix a) It is receptive to sperm penetration at or near ovulation b) Act as sperm reservoir c) Protects spermatozoa from hostile environment d) It act as sperm filter. e) It participates in capacitation f) It provide spermatozoa energy

spermatozoa from hostile environment d) It act as sperm filter.

9 Mid heat in Buffalo, uniformly injected capillaries - poor Hb levels ends up in Anovulatory heat

10 FEMALE REPRODUCTIVE ANATOMY

12 Cervix

14 CL GF

17 central depression crown of CL is protruded

18 Left CL & Right follicle - midcycle heat / early pregnancy

19 large follicle & CL in the same ovary gestational heat, seen in mid cycle heat AI is contra indicated.

20 Cow in Proper Time for Insemination

21 Thawing

22 Thawing Lifting of Canisters Keep thermometer in water, add hot water, temp. of water at 37OC. Dipping fingers testing temp - is wrong 8-10 cms distance between the top end of semen straw & mouth of LN2 container Use bent forceps - shake the straw add to water at 37OC.

23 Lead the animal into travis - pass lead rope over poll region, talk to the animal, be massaging on head or body. Cow - flap of flank can be held Give rest (15mts) before & after AI or adopt door step AI - higher CR. Avoid excitement during AI Highly excited animal - primary peritonitis after AI next day off feed is noticed Pouring cold water on the body - helps sperm propulsion

24 AI Deposition cranial to cervix

25 Tips for Fertility Training of AI Workers Time of AI- Thumb Rule Thawing method Wiping of Straw after thawing Small Jerk- Cut LN Level Temp of Gun Semen Deposition junction of cervix and body of uterus Avoid lubrication with Soap

26 Handling Containers LN Containers - doubled walled metal (Stainless Steel/Aluminum) vacuum vessels - very efficient insulation system Index ring to hold canisters in position (1), Neck tube (2), Molecularsieve absorbents (3), Inner vessels (4), Canister (5), Outer vessels (6) and Neck plug (7).

27 Handling Containers Avoid direct exposure to sun rays or hot air blast. Store the LN2 container in a cooler place. Always keep LN2 container in a dry place on some jute carpet or wooden plank to avoid a direct contact with the hard surfaces. Contact with wet/damp surface, cement floor as well as the chemicals may cause corrosion and ultimately lead to vacuum loss. Avoid all sorts of shocks like dropping/rough handling. Always lift the container with both the hands and set it down gently. Extensive shock may create a pendulum type vibration in the inner vessel and may lead to breakage at the neck tube junction.

28 Handling Containers

29 Handling Containers Index ring to hold To avoid splashing of LN2, do not tilt/roll the container. This will also prevent dents/scratches which lead to vacuum loss. The container can also be shifted by platform base trolley

30 Handling Containers Do not stack containers one above other. This may result in inadequate release of gaseous nitrogen causing pressure in the vessel. Increased pressure in the container may lead to explosion

31 Handling Containers Always keep the container closed with neck plug to minimize the LN2 loss. While opening, slide out the neck plug vertically. This will prevent possible damage of the neck plug.

32 Handling Containers Always keep the container vertically in a well ventilated room. Poorly ventilated room may lead to suffocation due to the decrease in atmosphere oxygen

33 Handling Containers To prevent the container from tipping, sliding and scraping during transportation, it is preferable to use wooden crate/hard board box with proper packing material. The container can also be secured with straps or with proper rubber band ring during transportation.

34 Handling Containers

35 Handling Containers Dip Stick method

ARTIFICIAL INSEMINATION

ARTIFICIAL INSEMINATION Artificial insemination is the deposition of male reproductive cells (spermatozoa) in the female reproductive tract by mechanical means rather than by natural mating. The success