Faculty of Economics. Pedagogical Faculty. Faculty of Science. Institute of Physical Biology

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1 University of South Bohemia Faculty of Agriculture České Budějovice, Czech Republic Faculty of Agriculture Faculty of Economics Faculty of Health and Social Studies Pedagogical Faculty Faculty of Philosophy Faculty of Science Faculty of Theology Research Institute of Fish Farming and Hydrobiology ese c s u e o s g d yd ob o ogy Institute of Physical Biology

2 Faculty of Agriculture Plant production Livestock breeding and genetics Agroecology Quality of feedstocks (for food industry)

3 Selected topics of interest Biogenic amines and polyamines Sulfur-containing secondary metabolites Patatin, the major potato tuber protein Flavonoids Processing of phytomass, green refinery e

4 Biogenic amines and polyamines N CH 2 CH 2 NH 2 CH 2 CH 2 NH 2 CH 2CH 2NH 2 Histamine Tryptamine Tyramine N H N H OH H 2 N NH NH 2 H N N 2 2 H H 2 N N H N H NH 2 Putrescine Spermidine Biogenic amines are determined for two reasons: Spermine Toxicological importance: Aromatic amines (e.g. histamine, tyramine) are allergy-like substances, causative factors of migraine. Polyamines support the proliferation of tissues. Polyamine rich diet is unsuitable for cancer patients. On the other hand, polyamine rich diet is required for patients after surgery, injuries. Food quality markers: In non-fermented foods, biogenic amines can serve as food quality markers, because their content is related to decomposition of proteins. Results: The team from Faculty of Agriculture successfully solved Czech Science Foundation (GAČR) Č projects and participated in two European COST projects aimed at Biogenic amines and Polyamines. Critical levels of amines were found for many feedstocks (mainly fresh water fish flesh). Polyamines content was determined in beef, pork, chicken and pluck after slaughter and after various storage conditions and cooking treatments.

5 Sulfur-containing secondary metabolites important flavor precursors of alliaceous plants (garlic, onion etc.) precursors of many health-benefitial compounds We focus on: development of analytical methods (CE, HPLC) studying their biogenetic pathways their changes during growth cycle and subsequent storage their role in formation of sensory-active compounds

6 Patatin, the major potato tuber protein Complex of patatin glycoproteins (39-43 kda) major glycoprotein complex of potato tubers protein with high nutritive value and interesting biochemical and functional properties We focus on: development of methods for patatin ti detection, ti purification and analysis (LC, FPLC, SDS-PAGE, chip electrophoresis etc.) patatin cultivar/genotype ypevariability content, isoforms, o s, biochemical c properties effect of agro-ecological factors on patatin characteristics patatin industrial isolation from waste potato juice

7 Flavonoids secondary metabolites of plants compounds with strong anti- oxidation and free radical scavenging activity protection against atherosclerotic and cancer We are interested in: CZE/MECC and HPLC methods for analysis of quercetin glycosides and other flavonoids determination of total quercetin and rutin content in fruits and vegetables, tea, beer and wine, buckwheat, amaranth and medicinal plants

8 Processing of phytomass according to the principle of green biorefinery Standard processing of agricultural raw materials is mostly intended to get one final product, e.g. sugar beet sugar, wheat flour and starch, energetic crops energy from phytomass. Modern approach - green biorefineries, on the contrary, works towards a complex and full use of a plant raw material including all production wastes. It is concerned with waste-free technologies. The working group from Faculty of Agriculture presented technological projects in form of patents and utility patterns for 22 final products from oats (Avena sativa), 6 products from clover (Trifolium pratense) and 8 products from sea-buckthorn (Hippophae rhamnoides) and 4 products from purple coneflower (Echinacea purpurea) with processing of all wastes by anaerobic digestion to biogas, fuel pellets and cheap organic-mineral i fertilizers. At present we work on a complex use of sunflower (Helianthus annuus), melilot (Melilotus albus) and barley (Hordeum vulgare).

9 Thank you for your attention

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