# TopoICE-X Manual. In these examples, we will use the following knots:

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3 You can display more knots by using the slider to change the zoo size or clicking on "zoo up" or "zoo down". There are two display modes: If "cat" zoo mode is selected, catalog type pictures of knots [4] will display. If "rat" zoo mode is selected, the knot is drawn using rational tangle notation. To enter a knot using the rollers (for those familiar with rational tangle notation): Enter the fraction corresponding to the rational knot N(A/B) by using the top two rollers. You can also enter these numbers by double clicking the number, typing in a new number, and hitting return. 3.) To find knots of crossing distance one from N(A/B) using the button marked "Find Now". Example 1: Find all possible rational knots which can be obtained from a given knot, for example 3.1 = N(3/1) =, via one round of topoisomerase action: Enter the knot 3.1 = N(3/1) by using "Load Zoo" or rollers: To find knots which can be obtained from the above knot via one round of topoisomerase action, click on "Find Now": 3

4 This will find all rational knots up to 13 crossings which can be obtained from the rational knot N(A/B) by changing one crossing. They will be displayed in the KnotPlot window by crossing number with the smallest crossing number knots listed first. For example, the following are knots of crossing distance one from the three crossing knot 3.1 = N(3/1): Click on any of the knots to see how the knot 3.1 = N(3/1) can be converted into this knot via a crossing change. For example, if the unknot = 0.1 = N(1/1) is selected, the KnotPlot window will display the following: The top two figures show the trefoil knot. In the top left figure, a circle is drawn around the crossing which will be changed to give the knot in the bottom right. In this case an unknot results. The figures on the right are simplified figures of the ones on the left. 4

5 To see more solutions: "up" or "down" buttons: Note there could be more than one way to transform one knot into another knot via a crossing change. Click on the grey box marked "up" or "down" to see the different ways to convert between two given rational knots. "next" and "prev" buttons: Click on the grey box marked "next" to see the next knot in the list of rational knots of crossing distance one from the rational knot N(3/1). Click on the grey box marked "prev" to see the previous knot in the list of rational knots of crossing distance one from the rational knot N(3/1). The notation and mathematics of rational tangles is used to draw these diagrams resulting in diagrams which could be significantly improved for biological modeling. However, one can use the energy functions within Knotplot to simplify diagrams. For more information on these energies, click on the help button on the Dynamics panel or see Rob Scharein s thesis, available at knotplot.com/thesis, for more information on these energies. Clicking on one of the green "Get" buttons will choose the diagram closest to the get button that you clicked and take you to the TopoICE -Dynamics page where you can run energies. For example, clicking on the upper left "Get" button results in the following: Click on "relax inside" to relax the tangle inside the sphere. Click on "relax outside" to relax the tangle outside the sphere. Click "relax all" to relax the entire knot. To speed up the calculations, 5

6 change dstep (display step). A redraw of the knot occurs every dstep dynamical step. The radius of the sphere can be changed using the radius slider. Uncheck auto set before attempting to change the radius. To return to the TopoICE-X panel, click on either the back button (top of KnotPlot control panel) or the button marked " TopoICE-X" (bottom of KnotPlot control panel). 4.) To determine if it is possible to convert a given knot into another knot via a crossing change. Example 2a: Is it possible to convert N(5/1) = into N(3/1) =? Enter first knot as described above. Enter second knot using either the load zoo button or the rollers for the second knot: Solution #1 of 1 appears in the KnotPlot window: By one solution, we mean that there is exactly one way, topologically speaking, to convert N(5/1) into N(3/1) via a single crossing change. However, any of the 5 crossings in this drawing of the 6

7 knot N(5/1) can be changed to obtain N(3/1). Hence it might look like there are 5 different ways to convert N(5/1) into N(3/1) via a crossing change. However, they are all topologically equivalent in that no matter which crossing is changed, the configuration outside of where the crossing change occurs are all topologically equivalent if the geometry of the bound crossing is the same. In fact, if knot energies were run to minimize the energy of the outside configuration while keeping the crossing fixed, then they would all minimize to identical configurations no matter which crossing is fixed: Example 2B: Is it possible to convert N(5/3) = into the unknot = N(1/1) = Enter the two knots as described in example 1A. Solution #1 of 2 appears in the KnotPlot window. In this case there are two topologically distinct ways to convert N(5/3) into N(1/1). Click on "up" or "down" buttons to see more ways of converting N(5/3) into N(1/1) via a single crossing change. For example, the second solution appears in the KnotPlot window by clicking on "up": Solution 1: Solution 2: We can mathematically prove that these two ways of converting the figure 8 knot = 4.1 = N(5/3) into the unknot = N(1/1) are topologically different. We can also see that these are topologically different solutions by relaxing the configuration outside of where the crossing change occurs. For example, for each of these two solutions, if we click on "get" and "relax outside" as discussed in example 1, we obtain the following: 7

8 Solution 1: Solution 2: Note that the configuration outside the sphere for solution 1 looks different than the configuration outside the sphere for solution 2. This is not a mathematical proof that they are different, but a proof does exist whenever KnotPlot finds more than one solution. 5.) Finding Pathways. Example 3: Is it possible to convert the knot 3.1 = N(3/1) = into the knot 4.1 = N(5/3) = If it is not possible, find pathways between these knots. Enter the two knots as described in examples 1 and 2a. Click on "Find solutions". If it is possible to change the first knot into the second knot via a crossing change, a solution showing the crossing change will appear in the KnotPlot window as described above. If it is not possible to change the first knot into the second knot via a crossing change, "It is not possible to convert N(3/1) into N(5/3) via a single crossing change." will appear in both the KnotPlot and the KnotPlot Command Windows: Click on the green find path between N(3/1) into N(5/3) to obtain all shortest pathways between these knots involving 8

9 rational knots with 13 or fewer crossings. Note there could be shorter pathways if nonrational knots were allowed. If the unknot, N(1/1) was selected as the first knot and if the five crossing knot N(5.1) was chosen to be the second knot, then the following will appear in the KnotPlot window: Click on load images (bottom right of KnotPlot window) to load knot images. Click on show in rat mode (bottom center) to display images using rational tangle notation or click on show in cat mode to display catalog-like images [4] when the crossing number of the knot is less than 11. Click on reverse (bottom left) to reverse the pathway or click on random knot pair to see pathways between a randomly chosen pair of knots. Click on an edge to see the conversion via a crossing change from the upper knot to the lower knot connected by this edge. 9

10 The last six requested reaction pathways are stored and can be easily accessed by clicking on the desired reaction pathway button on the KnotPlot control panel: 6.) Reference. [1] Adams, Colin C. The knot book. An elementary introduction to the mathematical theory of knots. American Mathematical Society, Providence, RI, xiv+307 pp. ISBN: [2] Kawauchi, Akio A survey of knot theory. Birkhäuser Verlag, Basel, xxii+420 pp. ISBN: [3] Livingston, Charles Knot theory. Carus Mathematical Monographs, 24. Mathematical Association of America, Washington, DC, xviii+240 pp. ISBN: [4] Rolfsen, Dale Knots and links. Mathematics Lecture Series, 7. Publish or Perish, Inc., Houston, TX, xiv+439 pp. ISBN: [5] [6] [7] [8] Burde, Gerhard; Zieschang, Heiner Knots. Second edition. de Gruyter Studies in Mathematics, 5. Walter de Gruyter & Co., Berlin, xii+559 pp. ISBN: [9] Darcy, Isabel K. Biological distances on DNA knots and links: applications to XER recombination. Knots in Hellas '98, Vol. 2 (Delphi). J. Knot Theory Ramifications 10 (2001), no. 2, [10] Goldman, Jay R.; Kauffman, Louis H. Rational tangles. Adv. in Appl. Math. 18 (1997), no. 3,

11 7.) TopoICE-X control panel You can also load a knot by entering its fraction and hitting return. Click to find all rational knots of distance 1 from the first knot Click find solutions to determine if it is possible to convert the first knot into the second knot via one crossing change. Load first knot Find shortest paths to unknot involving rational knots (note there could be shorter paths involving nonrational knots). Up to six pathways are stored and can be viewed by clicking one of these boxes. Enter second knot using load zoo or fraction If a solution is found, you can view different solutions using these buttons. These buttons allow one to toggle through solutions for knots of distance 1 from first knot. Determines number of knots displayed per row To display more knots One can display knots using rational tangle notation or as in Rolfsen s catalog. 11

12 8.) KnotPlot summary. TopoICE-X is part of KnotPlot which has a variety of tools for manipulating and visualizing knots. A brief summary of the control panels available in KnotPlot is given below. For more information about these control panels, click on that control panel s help button Control Panels: Main: See p. 13 Display: to change display settings. Dyna: can change settings for various forces that affect the dynamical behaviour of knots when the go button is clicked. The go button changes the conformation of a knot based upon the selected dynamics. Frequently used to simplify a knot diagram. TopoICE: Includes TopoICE-X for modeling topoisomerase action, TopoICE-R for modeling recombinase action, TopoICE-Dyna for running dynamics on tangle solutions found by TopoICE. The tangle calculator is not part of TopoICE, but can be accessed from this panel. The tangle calculator can be used to draw a variety of tangles including rational tangles. Sketch: For sketching a knot. Export: To export figures in a variety of formats including eps, bitmap, and 3D surface models. Edit: To edit a knot. For example, one can delete/add/move vertices and edges. 4D: 4-dimensional knots. Comp: For computing physical properties, topological invariants, etc of knots. Surf: To change coloring of knots. Cons: To construct a torus knot, Lissajous knot, chains, braids. Cat: To load a knot from catalogues of knot DemoA: A number of demos are available. ACN and rat tang may be of particular interest. DemoB: More demos. 12

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