OBTAINING MONOCLONAL ANTIBODIES TO EPITOPES OF IVERMECTIN

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "OBTAINING MONOCLONAL ANTIBODIES TO EPITOPES OF IVERMECTIN"

Transcription

1 С.Сейфуллин атындағы Қазақ агротехникалық университетінің Ғылым жаршысы / Вестник науки Казахского агротехнического университета им. С. Сейфуллина (79). C.8-14 OBTAINING MONOCLONAL ANTIBODIES TO EPITOPES OF IVERMECTIN Bulashev A.K., Akibekov O.S. Abstract Ivermectin belongs to the low molecular weight compounds that are not capable of independently eliciting an immune response due to the small size of its molecule. In order to give the hapten s molecule ability to cause antibody formation it must be conjugated with a macromolecular carrier. Balb/c mice were immunized with cоllodial gold and Ivermectin (CGI) conjugate through two schemes with a duration of immunization 16 days and 80 days. Both methods turned to be applicable for inducing antibody formation against Ivermectin. Two stable strains of Hybridomas producing monoclonal antibodies to epitopes of Ivermectin are obtained which can be used in the development of immunoassays for the rapid determination of the anthelmintic in animal products. Keywords: ivermectin, colloidal gold, immunization, hybridomas, monoclonal antibodies Introduction Ivermectin is the active substance of preparations (Ivomek, Baymek, Aversekt, Novomek, Ivertin, Iversect, Ivermek, Narvak, Rustomektin and others) which are widely used for the destruction of nematodes, scabies mites, gadfly larvae and other parasites in farm animals. The preparations of Ivermectin have a low toxicity for an organism and decent environmental properties. However, uncontrolled using them in animal husbandry represents a threat to human health because this group of preparations may be excreted with milk, accumulate in the organs and tissues of animals. In the case of non-compliance with the holding time of livestock after treatment with Ivermectin can enter the human body with products of animal origin and cause various pathologies [1]. At the present time to control of residual amounts of Ivermectin in foods High Performance Liquid Chromatography (HPLC) with fluorescence detector and its various modifications are mainly used [2]. However, drawbacks of the method, such as high cost of equipment, routine sample preparation, duration of analysis limit its widespread use. Recently, as a substitute for the HPLC the options of one of the highly sensitive immunological tests ELISA based on the antibodies specific to Ivermectin are used. It has some significant advantages over HPLC such as specificity, speed and simplicity of setting the test, and relatively low cost of the assay procedure. However, ELISA can be used only in

2 laboratories equipped with special devices. In this regard, the development of simple, but specific tests designed for rapid detection of Ivermectin s residues in animal products is one of the urgent tasks of Veterinary science. Nowadays Immunochromatographic assay remains the single immunological method which allow to determine for a few minutes the presence or absence of specific substances in biological samples (whole blood, urine, serum or plasma, saliva, feces, etc.) However, the sensitivity and specificity of this test is largely determined by the nature of the antibodies. In this regard, monoclonal antibodies (mabs) that can be used as "reliable tool" in the development of rapid tests are the great theoretical and practical interest. However, the production of mabs against Ivermectin is not easy job. By its chemical composition Ivermectin refers to the class of the macrocyclic lactones and, as a hapten, is not capable of stimulating the immune system to synthesize antibodies. In order to impart immunogenicity to the hapten s molecule, i.e. ability to evoke antibody production it must be conjugated with a macromolecular carriers. For this purpose different substances were tested among which colloidal gold turned to be the most suitable [3,4,5]. It does not cause allergies, non-toxic and has immunomodulatory properties. In addition, the gold itself does not act as an antigen which can not be said about other carriers, keeps the composition of antigen molecules attached to it, and hence capable to present them to cells responsible for immunity in an unmodified form [6,7 ]. The purpose of researches was to get mabs determinants of Ivermectin, conjugated with colloidal gold. against antigenic Materials and methods Balb/c mice, X63-Ag myeloma cells line and the following reagents were used in the research: chemically pure preparation of Ivermectin (Sigma); IgG-Free, protease-free bovine serum albumin (Jackson Immuno Research); colloidal gold (~ 0.01% HAuCl4, ~ 1A520 unit / ml, a monodisperse particle, size 20nm, Sigma); hydrochloride 1-ethyl 3-(3-dimethylaminopropyl) carbodiimide - EDC (Sigma-Aidrich); 2 - mercaptoethanol (Ferak); complete and incomplete Freund's adjuvant (Sigma); RPMI (Sigma); hypoxanthine-guanine phosphoribosyl transferase - GGFRT; hepes (Sigma); solution of PEG 4000 with 10% dimethyl sulfoxide DMSO (Fluca); sodium pyruvate (Sigma); L -glutamine (Sigma); coomassie G -250 ( Диэм); ethyl alcohol 96 º; fetal calf serum (Hy Clon); pristane - 2,6,10,14-tetrametilpentadekan (Fluca); nitrocellulose membrane (Millipore), 1% agar (Difco) and set of reagents for ELISA. Conjugation of Ivermectin and polyclonal rabbit antibodies with colloidal gold was performed as described by Dykman L. A. et.al (2008) [8]. Immunization of Balb/c mice with colloidal gold and Ivermectin (C GI) conjugate was performed according to the following two schemes. By the first scheme (the short scheme) mice were injected intraperitoneally with 0.1 ml of CGI conjugate (concentration 1mg/ml) in a

3 volume of 0.1 ml complete Freund's adjuvant in the first day of immunization. On the 7th day of immunization immunogen was injected at the indicated dose in a solution of incomplete Freund's adjuvant and then immunization was continued for 11, 12, 13th days using only CGI conjugate in a buffered saline. The second immunization scheme (the long scheme) envisages fivefold immunization. The interval between the first, second and third immunization was two weeks. Fourth immunization was performed in three, and fifth - in four weeks after the previous administration of immunogen. CGI conjugate was injected intraperitoneally at a concentration of 0.2 mg/ml in a mixture with Freund's complete adjuvant for the first immunization and at the subsequent immunization it was administrated with Freund's incomplete adjuvant. Booster dose in a volume of 0.2 mg/ml per head without Freund's adjuvant was injected to mice in both groups 3 days before the isolation of immune lymphocytes. Indirect ELISA was used to determine antibodies titers in serum of immunized Balb/c mice against Ivermectin. Hybridization of X63-Ag myeloma cells and immune mice s splenocytes was conducted by G.Kohler and C.Milstein [9]. «Dot» variant of sandwich ELISA was used for determining that produce antibodies against Ivermectin. For this purpose, samples of the hybridoma culture fluids were applied to a nitrocellulose membrane. After blocking free sites of nitrocellulose paper with bovine serum albumin it was transferred to a solution of Ivermectin. The presence of antibody+antigen complex was deteсted by using polyclonal rabbit antibodies, conjugated with colloidal gold. Cloning hybridoma cells was performed by limiting dilution [10]. Hybrid cells are cultured in the peritoneal cavity of syngeneic mice to produce preparative amounts of mabs. Affinity of mabs was established by the method of J.Beaty et al. [11]. Determination of isotypes of mabs performed by ELISA using the set of antisera to the different classes of mouse immunoglobulin. Results The serological test results of mice immunized with CGI conjugate are shown in table 1. As seen from table 1, the conjugate comprising Ivermectin showed its immunogenicity to Balb/c mice. Both schemes showed their sufficient efficiency in stimulating humoral response of immune system to Ivermectin. The groups of mice did not differ significantly among themselves by serum antibodies titers. Table 1 Studying blood sera of Balb/c mice by ELISA

4 Immunization schemes Short scheme Long scheme Numbers of mice Duration of immuni-za tion Numbers of isolated splenocytes, mln.cells Antibody titers х10 6 1: days 3х10 6 1: х10 6 1: days 7 х10 6 1: х10 6 1: х10 6 1:6 400 * Note - The samples of blood serum from each mouse were investigated in five replicates. The table shows the prevailing titers. From the spleen of each mouse splenocytes were obtained in an amount of 3,0 mln.cells up to 10,0 mln.cells. The data of table 1 indicate that for hybridization 41.0 million spleen cells were collected. The first hybridization carried out using immune lymphocytes of mouse 5 taken immediately before the cell fusion, and for the second fusion frozen lymphocytes of mouse 1 were selected. At the first of hybridization the number of splenocytes and myeloma cells were and , i.e. cell ratio was equal to 1:5. After the fusion the cells were seeded in 384 wells of culture plates. Formation of hybrid cells was observed in 63 wells i.e. percentage of hybridomas output was low and amounted to 16.4%. Furthermore, none of the of hybrid cells showed activity against Ivermectin, ie they did not synthesize antibodies specific to this anthelmintic. Considering low yield of hybridomas in the preceding fusion, for the second hybridization myeloma cells and lymphocytes were taken in a ration of 1:8,5. Splenocytes of the mouse 1 cryopreserved in liquid nitrogen were taken for the second hybridization. The viability of defrosted spleen lymphocytes was rather high (90%). Increased percentage of lymphocytes in the mixture of cell-partners positively affected on the hybrid output. Thus, the growth of hybrid cells was observed in 155 out of 384 seeded wells, or 40.4% of cases. The results of studying hybrid for antibody activity by ELISA showed the presence of mabs in the culture fluid of 4 hybridoma strains designated as 1H1, 1H9, 2A3 and 4B1. These positive were transferred to 24-well plates for further culturing. After accumulating a sufficient cell mass all hybridomas were cloned by limiting dilution to obtain genetically homogeneous lines. Antibody activity of sub was determined by ELISA in days after cloning. In this case the culture fluid samples were taken only from those wells where there were

5 microscopically distinguishable single cell colonies. Cloning results shown in table 2. Num-b ers of Table 2 - Activity of sub at the first cloning of hybridomas Designation of Numbers of formed sub total active sub 1 1Н (36,6%) 2 1Н (39,1%) 3 2А (27,7%) 4 4B (53,8%) Table 2 shows that at the first cloning the activity of sub, ie the ability to produce antibodies was rather low. Thus, in the supernatant of sub from hybridoma 4B1 specific antibodies were identified in 53.8% of cases, and the share of active sub from other three hybridomas (1H1, 1H9 and 2A3) was much lower: 36.6%, 39.1% and 27.7%, respectively. Taking into account the genetic heterogeneity of hybridoma after getting a sufficient amount of cells recloning of sub was immediately carried out (Table 3). Num-b ers of Table 3 - Activity of hybridomas sub after the second cloning Designation of Numbers of formed sub total Proportion of active 1 1Н (0%) 2 1Н (54,5%) 3 2А (0%) 4 4B1 9 5 (55,6%) Table 3 shows that the cells of two hybridomas (1H1 and 2A3) had lost the ability to synthesize mabs during cloning procedure. Hybrid sub 1H9 and 4B1 maintained their antibody activity. Thus, if the share of active sub of the first hybridoma was higher (54.5%) than that of in the first cloning (39,1%), then this indicator of the second one remained almost unchanged (53.8% and 55.6%). The resulting sub were cultured for one week, and then were subjected to the secondary recloning. The growth of isolated sub of hybridomas 1H9 and 4B1 was noted in 19 and 22 wells respectively and in all cases antibodies to Ivermectin had been registered. Thus, as a result of repeated recloning two of hybridomas were obtained producing mabs specific for antigenic determinants of Ivermectin.

6 For the accumulation of mabs in vitro hybridomas cells were transferred from the wells of 96-well to 24-well plates. With the accumulation of cells and the transition to the stage of logarithmic growth hybridomas were transferred and cultured in polystyrene mattresses. Antibody titers remained stable and were within 1:8-1:16. Protein concentration determined by Bradford s method was in the range of ug/ml. The cells of mabs producers were injected into the peritoneal cavity of four syngeneic mice (two heads per hybridoma), pretreated with pristane at a dose of 0.5 ml per head in the amount of 0.5 million cells per 1 ml. After two or three weeks, with the tumor formation ascitic fluid from the peritoneal cavity was aspirated. Table 4 shows some properties of the mabs which are synthesized by two of hybridomas. Table 4 - Characteristics of hybridoma 1Н9 и 4B1 Designation of Affinity Isotypes of mabs Produc-tivity of mabs in vitro, mg / ml Produc-tivity of mabs in vivo, mg / ml * mabs titers in the culture mediu m * mabs titers in ascitic fluid 1Н9 2,0х10-9 М IgG1 0,06 4,0 1:16 1: B1 2,6х10-9 М IgG1 0,03 4,0 1:8 1:800 * Note: Samples of the culture and ascitic fluids were tested by ELISA in five replicates. The table shows predominant titles. The results of studing individual properties of mabs showed that antibodies synthesized by the 1H9 and 4B1, belong to IgG1 and had a binding constant (affinity) with Ivermectin which is equal to 2.0x10-9 М and 2.6x10-9 М, respectively. As seen from the table 4, the hybridoma in a cell culture and ascitic fluid characterized by satisfactory productivity. Cultivating hybridomas in the peritoneal cavity of mice allowed to receive ascites fluid with the antibodies titers against Ivermectin within 1:800-1:1600. The samples of both mabs were subjected to SDS-PAGE electrophoresis on the apparatus for vertical electrophoresis to determine the molecular mass of immunoglobulins. The results showed that the heavy and light chains of mabs constitute the bands corresponding to 66 and 25 kd. Discussion and conclusion Ivermectin belongs to the low molecular weight compounds that are not capable of independently eliciting an immune response due to the small size of the molecule. In order to give the hapten s molecule ability to cause antibody

7 formation it must be conjugated with a macromolecular carrier. Immunization is the most "creative" stage in the process of obtaining antibodies, because its effectiveness determines ultimate success of the research. Balb/c mice were immunized with CGI conjugate through two schemes with a duration of immunization 16 days and 80 days. Both methods turned to be applicable for inducing antibody formation against Ivermectin "presented" to the immune system of Balb/c mice by colloidal gold. In serum samples of Balb/c mice antibodies against Ivermectin were detected in ELISA until dilutions of 1:6400-1: The lymphocytes isolated from spleens of immune mice were used for fusion with myeloma X63-Ag to produce hybrid - producers of mabs. Four hybrid synthesizing mabs to Ivermectin were obtained. Hybridomas were subjected to cloning and recloning in order to obtain lines which free from cells that lost their ability to synthesize of mabs. At the first cloning the activity of sub, i.e. the ability of cells to produce of antibodies was rather low. The share of sub that kept an antibody activity after the first cloning ranged from 27.7% up to 53.8%. These results indicate the presence of "descendants" among hybridoma cells that have lost their ability to produce antibodies as a result of mutation. Two hybridomas (1H1 and 2A3) which had a high percentage of passive sub following the results of first cloning lost their activity against Ivermect during the recloning. Sub of hybridomas 1H9 and 4B1 retained their ability to synthesize mabs after recloning. Moreover, the share of active sub of the first hybridoma was significantly higher (54.5%) than that of in primary cloning (39.1%). Hybridomas selected for further studies were cultured in vivo and in vitro for accumulation of preparative amounts of mabs specific to Ivermectin s epitopes followed by immunochemical study of their properties such as specificity, antibody activity, class and subclass of immunoglobulins, molecular weight of light and heavy chains of immunoglobulins and the binding constant of antibodies with the antigen (affinity). Monoclonal antibodies which are synthesized by cells of 1H9 and 4B1 belong to the class IgG1 and have affinity for Ivermectin equal to 2.0x10-9 М and 2.6x10-9 М with the titers of ascitic fluid 1:800 and 1:1 600, respectively. The concentration of mabs in the culture fluid was in the range of 0,03-0,06 mg/ml, but in ascitic fluid it reached 4.0 mg/ml. Thus, two stable strains of hybridomas were obtained producing mabs that can be used in the development of immunoassays for the rapid determination of Ivermectin in animal products.

8 References 1 Chicioine A.L.Ivermectin use and resulting milk residues on 4 Canadian dairy herds/d.durden, G.MacNaughton, and P.Dowlin//Can.Vet.J V.48/-P Danaher M. Review of methodology for the determination of macrocyclic lactone residues in biological matrices./ Howells L.C., Crooks S.R., Cerkvenik-Fläjs V., O'Keeffe M.// J. Chromatogr., Analyt. Technol. Biomed. Life Sсi P Дыкман Л.А. Золотые наночастицы: синтез, свойства, биомедицинское применение/л.а.дыкман, В.А.Богатырев, С.Ю.Щеглов, Н.Г.Хлебцов.-М.: С. 4 Dykman L. A. Immunogenic Properties of Colloidal Gold /M.V. Sumaroka, S. A. Staroverov, I. S. Zaitseva, V. A. Bogatyrev//Biology Bulletin of the Russian Academy of Science.-January V.31.-P Dykman L. A. Gold nanoparticles in biomedical applications: recent advances and perspectives/ Khlebtsov N.//Chem.Soc.Rev V.41.-P Pristensky D. V. Analysis of effectiveness of intracellular penetration of ivermectin immobilized onto corpuscular carriers.s. A. Staroverov, D. N. Ermilov, S. Yu. Shchyogolev, L. A. Dykman//Biochemistry (Moscow) Supplement Series B: Biomed.Chemistry.- September V.1.-P Pristensky D. V. Effect of gold nanoparticles on the respiratory activity of peritoneal macrophages Gold Bulletin/S. A. Staroverov, D. N. Ermilov, S. Yu. Shchyogolev, L. A. Dykman.- June V.42.- P Дыкман Л.А. Золотые наночастицы: синтез, свойства, биомедицинское применение/ Л.А.Дыкман, В.А.Богатырев, С.Ю.Щёголов, Н.Г.Хлебцов; Институт биохимии и физиологии растений и микроорганизмов РАН. М.:Наука, С Kohler G and Milstein C. Continuous culture of fused cell secreting antibody of predetermined specificity //Nature V P McKearn T.J. Цит.по Р.Г.Кеннету,Т.Дж.Маккерну и К.Б.Бехтолую Моноклональные антитела. Гибридомы: новый уровень биологического анализа. Перевод с англ. М.:Медицина, С. 11 Beatty J., Beatty P. and Vlahos W. Measurement of monoclonal antibody affinity by non-competitive immunoassay.- J.Immunol. Meth V P

9 Резюме Ивермектин относится к низкомолекулярным веществам, которые самостоятельно не способны вызывать иммунный ответ организма. Для придания молекуле гаптена способность стимулировать антителообразование его необходимо конъюгировать с высокомолекулярными носителями. Мыши линии Balb/c были иммунизированы конъюгатом, приготовленным из ивермектина и коллоидного золота, по двум схемам с продолжительностью иммунизации 16 и 80 дней, соответственно. Оба метода оказались пригодными для индуцирования образование антител против ивермектина. С помощью метода гибридомной техники были созданы два стабильных штамма гибридных культивируемых клеток, продуцирующие моноклональные антитела к эпитопам ивермектина. Полученные антитела могут быть использованы в разработке иммунологических тестов для экспресс-определения остаточного количества антгельминтика в животноводческих продуктах. Түйін Ивермектин өздігінен организмнің иммундық жауабын тудыра алмайтын молекулярлық салмағы жеңіл заттар қатарына жатады. Гаптен молекуласына иммуногендік қасиетті беру мақсатында оны жоғарымолекулярлық тасымалдағыштармен конъюгациялау керек. Balb/c линиясының тышқандары ивермектин мен коллоидты алтынның қоспасынан әзірленген конъюгатты 16 және 80 күн аралығында егу арқылы иммунизацияланды. Сыналған тәсілдердің екеуі де организмнің ивермектинге қарсы антидене түзуін қоздыра алды. Гибридома техникасының көмегімен ивермектиннің эпитоптарына телімді моноклоналдық антиденелерді тұрақты түрде түзейтін гибридті жасушалардың екі штамы алынды. Мұндай антиденелер мал өнімдерінен даярланған тағамдарда антгельминтиктің қалдық мөлшерін тез арада анықтай алатын иммунологиялық тәсілдерді әзірлеуде қолданыс табуы мүмкін.

SCANTIBODIES Laboratory, Inc. Contract Monoclonal Antibody Production

SCANTIBODIES Laboratory, Inc. Contract Monoclonal Antibody Production A Technical Publication of SCANTIBODIES Laboratory, Inc. Volume 1 Number 4 9336 Abraham Way Santee, CA 92071 USA (619) 258-9300 fax (619) 258-9366 www.scantibodies.com SCANTIBODIES Laboratory, Inc. Contract

More information

Antibody Services. Best Guarantees in the Industry! Monoclonal Antibody Services. Polyclonal Antibody Services. Express Antibody TM Services

Antibody Services. Best Guarantees in the Industry! Monoclonal Antibody Services. Polyclonal Antibody Services. Express Antibody TM Services Antibody Services Best Guarantees in the Industry! Monoclonal Antibody Services Polyclonal Antibody Services Express Antibody TM Services Guaranteed Antibody Services Antibody Purification Services Your

More information

Custom Antibodies Services. GeneCust Europe. GeneCust Europe

Custom Antibodies Services. GeneCust Europe. GeneCust Europe GeneCust Europe Laboratoire de Biotechnologie du Luxembourg S.A. 6 rue Dominique Lang L-3505 Dudelange Luxembourg Tél. : +352 27620411 Fax : +352 27620412 Email : info@genecust.com Web : www.genecust.com

More information

Custom Antibody Services

Custom Antibody Services Custom Antibody Services Custom service offerings DNA sequence Plasmid Peptide Structure Protein Peptide Small molecule Cells Spleen Lymphocytes Antigen Preparation Immunization Fusion & Subcloning Expansion

More information

KMS-Specialist & Customized Biosimilar Service

KMS-Specialist & Customized Biosimilar Service KMS-Specialist & Customized Biosimilar Service 1. Polyclonal Antibody Development Service KMS offering a variety of Polyclonal Antibody Services to fit your research and production needs. we develop polyclonal

More information

Chapter 18: Applications of Immunology

Chapter 18: Applications of Immunology Chapter 18: Applications of Immunology 1. Vaccinations 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology 1. Vaccinations What is Vaccination? A method of inducing artificial immunity by exposing

More information

Frequently Asked Questions (FAQs): Antibodies General

Frequently Asked Questions (FAQs): Antibodies General Frequently Asked Questions (FAQs): Antibodies General Should I choose mab or pab? The production of antibodies involves the immunisation of one or more animals with an antigen in order to elicit an immune

More information

CUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation

CUSTOM ANTIBODIES. Fully customised services: rat and murine monoclonals, rat and rabbit polyclonals, antibody characterisation, antigen preparation CUSTOM ANTIBODIES Highly competitive pricing without compromising quality. Rat monoclonal antibodies for the study of gene expression and proteomics in mice and in mouse models of human diseases available.

More information

3 months 1.5 months 1.5 months. 1 month

3 months 1.5 months 1.5 months. 1 month Rabbit monoclonal antibody (Mab) is secreted by the plasma B-cell of the rabbit. Traditional generation of rabbit Mab relies on a rabbit myeloma for B- cell fusion (

More information

MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project

MAB Solut. MABSolys Génopole Campus 1 5 rue Henri Desbruères 91030 Evry Cedex. www.mabsolut.com. is involved at each stage of your project Mabsolus-2015-UK:Mise en page 1 03/07/15 14:13 Page1 Services provider Department of MABSolys from conception to validation MAB Solut is involved at each stage of your project Creation of antibodies Production

More information

1. Vaccinations. What is Vaccination? Chapter 18: Applications of Immunology. 1. Vaccinations. 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology

1. Vaccinations. What is Vaccination? Chapter 18: Applications of Immunology. 1. Vaccinations. 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology Chapter 18: Applications of Immunology 1. Vaccinations 2. Monoclonal vs Polyclonal Ab 3. Diagnostic Immunology 1. Vaccinations What is Vaccination? A method of inducing artificial immunity by exposing

More information

STANDARD OPERATING PROCEDURE

STANDARD OPERATING PROCEDURE STANDARD OPERATING PROCEDURE Title: Antibody Production at Strategic Diagnostics Inc. SOP#: M-119 Version #: 1 Date Approved: August 6, 2009 Author: Strategic Diagnostic Inc. Date Modified: 1. PURPOSE

More information

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known

More information

Aviva Systems Biology

Aviva Systems Biology Aviva Custom Antibody Service and Price Mouse Monoclonal Antibody Service Package Number Description Package Contents Time Price Customer provides antigen protein $6,174 Monoclonal package1 (From protein

More information

Chapter 2 Antibodies. Contents. Introduction

Chapter 2 Antibodies. Contents. Introduction Chapter 2 Antibodies Keywords Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytochemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining

More information

Geniron. Custom Antibody Services. Serum services Antibody Monoclonal. Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody

Geniron. Custom Antibody Services. Serum services Antibody Monoclonal. Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody Geniron Custom Antibody Services Serum services Antibody Monoclonal Purification Antibody Mono Y Genetic Immunization Genbody Polyclonal Antibody Geniron Poly Y WE PROVIDE OUR SERVICES TO With Expertise

More information

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS Table of Contents 1. Purpose 2. Choice of Species and Strain 3. Immunizing Antigen 4. Procedures for Polyclonal Antibody Production 5.

More information

1.Gene Synthesis. 2.Peptide & Phospho-P. Assembly PCR. Design & Synthesis. Advantages. Specifications. Advantages

1.Gene Synthesis. 2.Peptide & Phospho-P. Assembly PCR. Design & Synthesis. Advantages. Specifications. Advantages 1.Gene Synthesis Assembly PCR Looking for a cdna for your research but could not fish out the gene through traditional cloning methods or a supplier? Abnova provides a gene synthesis service via assembly

More information

Interim Progress Report R&D Project 348. Development of a Field Test Kit for Detection of Blue-Green Algal Toxins

Interim Progress Report R&D Project 348. Development of a Field Test Kit for Detection of Blue-Green Algal Toxins Interim Progress Report R&D Project 348 Development of a Field Test Kit for Detection of Blue-Green Algal Toxins Biocode Limited November 1992 R&D 348/04/A ENVIRONMENT AGENCY 135357 CONTENTS SUMMARY KEYWORDS

More information

CHAPTER 8 IMMUNOLOGICAL IMPLICATIONS OF PEPTIDE CARBOHYDRATE MIMICRY

CHAPTER 8 IMMUNOLOGICAL IMPLICATIONS OF PEPTIDE CARBOHYDRATE MIMICRY CHAPTER 8 IMMUNOLOGICAL IMPLICATIONS OF PEPTIDE CARBOHYDRATE MIMICRY Immunological Implications of Peptide-Carbohydrate Mimicry 8.1 Introduction The two chemically dissimilar molecules, a peptide (12mer)

More information

Elabscience Biotechnology Co.,Ltd

Elabscience Biotechnology Co.,Ltd Biotechnology Co.,Ltd Your one-stop custom antibody service Antibody is a kind of protein that is the most widely used in the fields of biology and medicine. Antibodies, as the preparations for disease

More information

Chapter 3.2» Custom Monoclonal

Chapter 3.2» Custom Monoclonal 198 3 3.2 Custom Monoclonal 199 Mouse monoclonal antibody development Chapter 3.2» Custom Monoclonal 200 In vitro monoclonals expression service 201 Mouse monoclonal antibody additional services 202 Magnetic

More information

Applications of Ab Molecules. Chapter 4 Monoclonal Ab (p.99) Chapter 5 Ab genes and Ab Engineering (p.128)

Applications of Ab Molecules. Chapter 4 Monoclonal Ab (p.99) Chapter 5 Ab genes and Ab Engineering (p.128) Applications of Ab Molecules Chapter 4 Monoclonal Ab (p.99) Chapter 5 Ab genes and Ab Engineering (p.128) Monoclonal Antibodies Clonal Selection of B Lymphocytes Hybridoma Köhler and Milsten (1975) - continuous

More information

CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS

CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS CHAPTER 2 ANTIGEN/ANTIBODY INTERACTIONS See APPENDIX (1) THE PRECIPITIN CURVE; (2) LABELING OF ANTIBODIES The defining characteristic of HUMORAL immune responses (which distinguishes them from CELL-MEDIATED

More information

Microbiology AN INTRODUCTION EIGHTH EDITION

Microbiology AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Microbiology AN INTRODUCTION EIGHTH EDITION Differentiate between innate and acquired immunity. Chapter 17 Specific Defenses of the Host: The Immune Response B.E Pruitt & Jane J. Stein

More information

MONOCLONAL ANTIBODY PRODUCTION

MONOCLONAL ANTIBODY PRODUCTION MONOCLONAL ANTIBODY PRODUCTION Antibodies having single specificity produced from a single clone of B cell are referred as Mono clonal antibodies (MAbs). In 1975, Georges Köhler and Cesar Milstein devised

More information

Basics of Immunology

Basics of Immunology Basics of Immunology 2 Basics of Immunology What is the immune system? Biological mechanism for identifying and destroying pathogens within a larger organism. Pathogens: agents that cause disease Bacteria,

More information

Production and purification of monoclonal and polyclonal antibodies. Human S100A8,

Production and purification of monoclonal and polyclonal antibodies. Human S100A8, Supplementary Data Methods Production and purification of monoclonal and polyclonal antibodies. Human SA8, SA9 and SA mouse monoclonal antibodies (mab) were generated using the ClonaCell H hybridoma cloning

More information

DELFIA assays bring convenience in monoclonal antibody development

DELFIA assays bring convenience in monoclonal antibody development DELFIA DELFIA assays bring convenience in monoclonal development INTRODUCTION The DELFIA system provides a method of discovering specific, high-affinity monoclonal antibodies (Mabs) even in low concentrations

More information

From Biology to Discovery

From Biology to Discovery POLYCLONAL ANTIBODY PRODUCTION SERVICES Abbiotec offers custom polyclonal antibody production services to the scientific community to accelerate a project From Biology to Discovery. When the antibody for

More information

Antibodies. Thomas Boenisch, MS, and Jim Hudson, PhD

Antibodies. Thomas Boenisch, MS, and Jim Hudson, PhD Thomas Boenisch, MS, and Jim Hudson, PhD Immunoglobulins The vertebrate immune system has the amazing ability to create millions of different antibodies with different specificities. It is their ability

More information

Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay

Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay Kasetsart J. (Nat. Sci.) 37 : 137-144 (2003) Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay Montri Punyatong, Puntipa Pongpiachan

More information

Advanced BioDesign Outlines Solutions. Antibody Overview. by Advanced BioDesign. Project Start. Immunogenicity. Selecting Your Antigen

Advanced BioDesign Outlines Solutions. Antibody Overview. by Advanced BioDesign. Project Start. Immunogenicity. Selecting Your Antigen Advanced BioDesign Outlines Solutions by Advanced BioDesign Antibody Overview Launching an immunisation programme is an important experimental step that needs care. With Advanced BioDesign, you may develop

More information

Chapter 2 Primary Antibodies

Chapter 2 Primary Antibodies Chapter 2 Primary Antibodies To a certain extent, IHC can be compared to GPS navigation: to find the place of interest, we must enter its address which is a unique identifier and the electronic navigator

More information

GenScript Antibody Services

GenScript Antibody Services GenScript Antibody Services Scientific experts, innovative technologies, proven performance We specialize in custom antibody production to empower your research Custom Polyclonal Antibody Production Custom

More information

Your partner in immunology

Your partner in immunology Your partner in immunology Expertise Expertise Reactivity Reactivity Quality Quality Advice Advice Who are we? Specialist of antibody engineering Covalab is a French biotechnology company, specialised

More information

Activation and effector functions of HMI

Activation and effector functions of HMI Activation and effector functions of HMI Hathairat Thananchai, DPhil Department of Microbiology Faculty of Medicine Chiang Mai University 25 August 2015 ว ตถ ประสงค หล งจากช วโมงบรรยายน แล วน กศ กษาสามารถ

More information

QED Bioscience Inc. Revision 05/04/11 ADVANCED ANTIBODY TECHNOLOGIES

QED Bioscience Inc. Revision 05/04/11 ADVANCED ANTIBODY TECHNOLOGIES CUSTOM POLYCLONAL ANTIBODY DEVELOPMENT QED is committed to excellence across all facets of our operations, and to responding to the ever-changing needs of our customers. We have been developing monoclonal

More information

CONTENT. Chapter 1 Review of Literature. List of figures. List of tables

CONTENT. Chapter 1 Review of Literature. List of figures. List of tables Abstract Abbreviations List of figures CONTENT I-VI VII-VIII IX-XII List of tables XIII Chapter 1 Review of Literature 1. Vaccination against intracellular pathogens 1-34 1.1 Role of different immune responses

More information

Antibody Production Price List

Antibody Production Price List Antibody Production Price List Presenting Insight Biotechnology s price list for custom polyclonal and monoclonal antibody production services. We are happy to tailor individual packages towards the specific

More information

Custom Antibody Services

Custom Antibody Services prosci-inc.com Custom Antibody Services High Performance Antibodies and More Broad Antibody Catalog Extensive Antibody Services CUSTOM ANTIBODY SERVICES Established in 1998, ProSci Incorporated is a leading

More information

ELITE Custom Antibody Services

ELITE Custom Antibody Services ELITE Custom Antibody Services ELITE Custom Antibody Services Experience, confidence, and understanding As a manufacturer and service provider, we have the experience, confidence, and understanding to

More information

The Use of Antibodies in Immunoassays

The Use of Antibodies in Immunoassays TECHNICAL NOTE The Use of Antibodies in Immunoassays Introduction Structure of an IgG Antibody Immunological reagents are the backbone of every immunoassay system. Immunoassays can be utilized to quantitatively

More information

Custom Polyclonal Anti-Peptide Antibody, Brochure

Custom Polyclonal Anti-Peptide Antibody, Brochure Custom Polyclonal Anti-Peptide Antibody, Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a

More information

Grundlagen und Anwendung der Genom- und Proteomforschung

Grundlagen und Anwendung der Genom- und Proteomforschung Grundlagen und Anwendung der Genom- und Proteomforschung Profilmodul (Vorlesung/Seminar WS 2012/13) Philipps-Universität Marburg Fachbereich Biologie Professor Dr. Egon Amann & Dr. Frank Vitzthum Gastbeiträge

More information

VETERINARY SERVICES MEMORANDUM NO. 800.90

VETERINARY SERVICES MEMORANDUM NO. 800.90 August 5, 1998 VETERINARY SERVICES MEMORANDUM NO. 800.90 Subject: To: Guidelines for Veterinary Biological Relative Potency Assays and Reference Preparations Based on ELISA Antigen Quantification Veterinary

More information

Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159. Date Submitted: 12-11-09

Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159. Date Submitted: 12-11-09 Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159 Investigator: Institution: Carol Wyatt Kansas State University Date Submitted: 12-11-09 Industry summary: Effective circovirus vaccines

More information

Mouse IFN-gamma ELISpot Kit

Mouse IFN-gamma ELISpot Kit Page 1 of 8 Mouse IFN-gamma ELISpot Kit Without Plates With Plates With Sterile Plates Quantity Catalog Nos. 862.031.001 862.031.001P 862.031.001S 1 x 96 tests 862.031.005 862.031.005P 862.031.005S 5 x

More information

Chapter 6: Antigen-Antibody Interactions

Chapter 6: Antigen-Antibody Interactions Chapter 6: Antigen-Antibody Interactions I. Strength of Ag-Ab interactions A. Antibody Affinity - strength of total noncovalent interactions between single Ag-binding site on an Ab and a single epitope

More information

perfectprotein Antibodies ntelechon

perfectprotein Antibodies ntelechon Antibodies ntelechon Milstein and Köhler: The hybridoma technology December 1974, Cambridge: Georges J.F. Köhler, a postdoctorial fellow of César Milstein, had given eternal life to normal antibody producing

More information

Monoclonal Antibody Supernatant and Ascites Fluid Production

Monoclonal Antibody Supernatant and Ascites Fluid Production Monoclonal Antibody Supernatant and Ascites Fluid Production UNIT 2.6 A major advantage of using monoclonal antibodies over polyclonal antisera is the potential availability of large quantities of the

More information

Immunisation techniques. Antibody (polyclonal and monoclonal) production, purification, labelling. Hybridoma technology, fermentation

Immunisation techniques. Antibody (polyclonal and monoclonal) production, purification, labelling. Hybridoma technology, fermentation Immunisation techniques. Antibody (polyclonal and monoclonal) production, purification, labelling. Hybridoma technology, fermentation Department of Immunology and Biotechnology University of Pécs Structure

More information

Florida State University Monoclonal Antibody Production in the Mouse

Florida State University Monoclonal Antibody Production in the Mouse Florida State University Monoclonal Antibody Production in the Mouse Monoclonal antibodies are important tools both for basic research as well as for medical treatment. Monoclonal antibodies are created

More information

Production and characterization of monoclonal antibodies for aflatoxin B1

Production and characterization of monoclonal antibodies for aflatoxin B1 Letters in Applied Microbiology 1999, 29, 284 288 Production and characterization of monoclonal antibodies for aflatoxin B1 K. T. Devi 1, M.A. Mayo 2, K.L.N. Reddy 2, P. Delfosse 1, G. Reddy 3, S.V. Reddy

More information

THE His Tag Antibody, mab, Mouse

THE His Tag Antibody, mab, Mouse THE His Tag Antibody, mab, Mouse Cat. No. A00186 Technical Manual No. TM0243 Update date 01052011 I Description.... 1 II Key Features. 2 III Storage 2 IV Applications.... 2 V Examples - ELISA..... 2 VI

More information

GenScript Antibody Services

GenScript Antibody Services GenScript Antibody Services Scientific experts, innovative technologies, proven performance We specialize in custom antibody production to empower your research Custom Polyclonal Antibody Production Custom

More information

Chapter 3. Immunity and how vaccines work

Chapter 3. Immunity and how vaccines work Chapter 3 Immunity and how vaccines work 3.1 Objectives: To understand and describe the immune system and how vaccines produce immunity To understand the differences between Passive and Active immunity

More information

CHAPTER 6 ANTIBODY GENETICS: ISOTYPES, ALLOTYPES, IDIOTYPES

CHAPTER 6 ANTIBODY GENETICS: ISOTYPES, ALLOTYPES, IDIOTYPES CHAPTER 6 ANTIBODY GENETICS: ISOTYPES, ALLOTYPES, IDIOTYPES See APPENDIX: (3) OUCHTERLONY; (4) AFFINITY CHROMATOGRAPHY Human immunoglobulins are made up of LIGHT and HEAVY chains encoded by a total of

More information

Generating Monoclonal Antibodies

Generating Monoclonal Antibodies CHAPTER 7 Generating Monoclonal Antibodies Edward A. Greenfield Dana-Farber Cancer Institute, Boston, Massachusetts 02115 INTRODUCTION Characteristics of Monoclonal Antibodies, 203 Production of Monoclonal

More information

Guidelines for Monoclonal Antibody Production

Guidelines for Monoclonal Antibody Production Guidelines for Monoclonal Antibody Production 2008 Guidelines for Monclonal Antibody Production 2008 Commonwealth of Australia 2008 Electronic documents This work is copyright. You may download, display,

More information

27E10: A unique monoclonal antibody against MRP8/14 (S100A8/A9, Calprotectin)

27E10: A unique monoclonal antibody against MRP8/14 (S100A8/A9, Calprotectin) BMA BIOMEDICALS Rheinstrasse 28-32 CH-4302 Augst (Switzerland) Phone:+41 61 811 6222 Fax: +41 61 811 6006 info@bma.ch www.bma.ch 27E10: A unique monoclonal antibody against MRP8/14 (S100A8/A9, Calprotectin)

More information

Antibody Services from GenScript

Antibody Services from GenScript Services from GenScript www.genscript.com GenScript USA Inc. 860 Centennial Ave., Piscataway, NJ 08854 USA Toll-Free: 1-877-436-7274 Fax: 1-732-210-0262 1-732-885-5878 Academic Services Pharmaceutical

More information

IV. Principles of Serological Testing in Immunohematology

IV. Principles of Serological Testing in Immunohematology IV. Principles of Serological Testing in Immunohematology A. Blood Group Antigens and Antibodies 1. Blood group antigens are structures embedded in or protruding from RBCs, WBCs and Platelets. 2. Exposure

More information

Catalogue 2009/2010. Immunological and biochemical services

Catalogue 2009/2010. Immunological and biochemical services Catalogue 2009/2010 Immunological and biochemical services 1 Customised Services Page Antisera and Polyclonal Antibodies 4 Guarantee offer - Rabbit antibodies with guaranteed quality 5 Inclusive offers

More information

In Vitro And In Vivo Production Of Antibodies

In Vitro And In Vivo Production Of Antibodies In Vitro And In Vivo Production Of Antibodies OCTOBER 27, 2015 BY ADMIN Overview An antibody is a protein normally produced by the B cells of the immune system. Their original purpose is to identify and

More information

Bio-manufacturing of antigens, antibodies and bioresearch materials

Bio-manufacturing of antigens, antibodies and bioresearch materials Bio-manufacturing of antigens, antibodies and bioresearch materials Foetal Bovine Serum (FBS) Human AB Serum Chick Embryo Extract (CEE) Custom polyclonal antibody production Custom monoclonal antibody

More information

Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and

Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere

More information

SCREENING, STABILIZATION AND EXPANSION OF SECRETORY HYBRIDOMAS IN CULTURE AS A STEADY SOURCE OF MONOCLONAL ANTIBODIES (Mabs).

SCREENING, STABILIZATION AND EXPANSION OF SECRETORY HYBRIDOMAS IN CULTURE AS A STEADY SOURCE OF MONOCLONAL ANTIBODIES (Mabs). SCREENING, STABILIZATION AND EXPANSION OF SECRETORY HYBRIDOMAS IN CULTURE AS A STEADY SOURCE OF MONOCLONAL ANTIBODIES (Mabs). Maddaly Ravi a Sukanya Shyama Sundar a, Kaavya Krishna Kumar a, Deepa Parvathi.V

More information

Production and Characterization of Monoclonal Antibodies to Rat Angiotensinogen

Production and Characterization of Monoclonal Antibodies to Rat Angiotensinogen Production and Characterization of Monoclonal Antibodies to Rat Angiotensinogen ERIC CLAUSER, WOLFGANG BAIER-KUSTERMANN, JACOB BOUHNIK, PIERRE CORVOL, JOEL MENARD, AND MARCO CELIO SUMMARY Three stable

More information

Methodological considerations: Potency tests for recombinant adjuvanted RTS,S vaccine

Methodological considerations: Potency tests for recombinant adjuvanted RTS,S vaccine Methodological considerations: Potency tests for recombinant adjuvanted RTS,S vaccine Background Adjuvanted recombinant RTS,S-based vaccines have been in clinical evaluation since the late 1980s and various

More information

Production of Monoclonal Antibody Supernatant and Ascites Fluid

Production of Monoclonal Antibody Supernatant and Ascites Fluid Production of Monoclonal Antibody Supernatant and Ascites Fluid Wayne M. Yokoyama 1 UNIT 11.10 1 Howard Hughes Medical Institute and Washington University School of Medicine, St. Louis, Missouri ABSTRACT

More information

SUMMARY AND CONCLUSIONS

SUMMARY AND CONCLUSIONS SUMMARY AND CONCLUSIONS Summary and Conclusions This study has attempted to document the following effects of targeting protein antigens to the macrophage scavenger receptors by maleylation. 1. Modification

More information

ELISA BIO 110 Lab 1. Immunity and Disease

ELISA BIO 110 Lab 1. Immunity and Disease ELISA BIO 110 Lab 1 Immunity and Disease Introduction The principal role of the mammalian immune response is to contain infectious disease agents. This response is mediated by several cellular and molecular

More information

Polyclonal and Monoclonal Antibodies

Polyclonal and Monoclonal Antibodies Polyclonal and Monoclonal Antibodies 23 2 Polyclonal and Monoclonal Antibodies Mary A. Ritter 1. Introduction The breadth of repertoire yet beautiful specificity of the antibody response is the key to

More information

TABLE OF CONTENT. Page ACKNOWLEDGEMENTS. iii ENGLISH ABSTRACT THAI ABSTRACT. vii LIST OF TABLES LIST OF FIGURES. xvi ABBREVIATIONS.

TABLE OF CONTENT. Page ACKNOWLEDGEMENTS. iii ENGLISH ABSTRACT THAI ABSTRACT. vii LIST OF TABLES LIST OF FIGURES. xvi ABBREVIATIONS. x TABLE OF CONTENT ACKNOWLEDGEMENTS ENGLISH ABSTRACT THAI ABSTRACT LIST OF TABLES LIST OF FIGURES ABBREVIATIONS iii iv vii xv xvi xviii CHAPTER I: INTRODUCTION 1.1 Statement of problems 1 1.2 Literature

More information

Monoclonal Antibodies

Monoclonal Antibodies Heddy Zola, Child Health Research Institute, Adelaide, Australia Monoclonal antibodies are antibodies with a unique specificity, generally made by cloning cells containing a particular antibody gene to

More information

DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development application Note TRF Technology for Biotherapeutics PerkinElmer, Inc. Hopkinton, MA USA DELFIA Assays: Flexible and Sensitive Tools for Antibody Development Excitation Fluorescence = 340 nm 0 400 800 1000

More information

The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small

The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small molecules that can elicit an immune response when linked

More information

Lecture Antibodies: structure, interaction, and production

Lecture Antibodies: structure, interaction, and production Principles and Practice of Protein Purification Lecture 6 5. Antibodies: structure, interaction, and production San-Yuan Huang Lab. Animal Proteomics Dept. Animal Science, NCHU Outlines of the lecture

More information

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi-

www.gbo.com/bioscience Tissue Culture 1 Cell/ Microplates 2 HTS- 3 Immunology/ HLA 4 Microbiology/ Bacteriology Purpose Beakers 5 Tubes/Multi- 11 Cryo 5 Tubes/Multi 2 HTS 3 Immunology / Immunology Technical Information 3 I 2 96 Well ELISA 3 I 4 96 Well ELISA Strip Plates 3 I 6 8 Well Strip Plates 3 I 7 12 Well Strip Plates 3 I 8 16 Well Strip

More information

Aviva Systems Biology

Aviva Systems Biology Aviva Custom Antibody Services and Prices Rabbit Polyclonal Antibody Service Package Number Description Package Contents Time Price Polyclonal package 1 (From protein to antiserum) Polyclonal package 2

More information

Antibody Function & Structure

Antibody Function & Structure Antibody Function & Structure Specifically bind to antigens in both the recognition phase (cellular receptors) and during the effector phase (synthesis and secretion) of humoral immunity Serology: the

More information

Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a

Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a Ryan S. Stowers, 1 Jacqueline A. Callihan, 2 James D. Bryers 2 1 Department of Bioengineering, Clemson University, Clemson,

More information

A customizable ADCC assay service for antibodies & fusion proteins.

A customizable ADCC assay service for antibodies & fusion proteins. Antibody Dependent Cell- Mediated Cytotoxicity (ADCC) Assay A customizable ADCC assay service for antibodies & fusion proteins. Our ADCC assay service accurately detects cell lysis based on LDH-release.

More information

Guide to Purification of Polyclonal Antibodies

Guide to Purification of Polyclonal Antibodies Guide to Purification of Polyclonal Antibodies When choosing a polyclonal antibody, either as a primary or secondary antibody in an immunoassay, researchers are often inundated with an array of antibody

More information

Antibody Services from GenScript, Brochure

Antibody Services from GenScript, Brochure Services from GenScript, Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a F NL +31 (0)26

More information

Covalent Conjugation to Cytodiagnostics Carboxylated Gold Nanoparticles Tech Note #105

Covalent Conjugation to Cytodiagnostics Carboxylated Gold Nanoparticles Tech Note #105 Covalent Conjugation to Cytodiagnostics Carboxylated Gold Nanoparticles Tech Note #105 Background Gold nanoparticle conjugates have been widely used in biological research and biosensing applications.

More information

SwellGel Immobilized Protein A, 96-Well Filter Plate

SwellGel Immobilized Protein A, 96-Well Filter Plate INSTRUCTIONS SwellGel Immobilized Protein A, 96-Well Filter Plate 89828 3747 N. Meridian Road P.O. Box 117 Rockford, IL 61105 1331w Number Description 89828 SwellGel Protein A, 96-Well Filter Plate, contains

More information

Dot Blot Analysis. Teacher s Guidebook. (Cat. # BE 502) think proteins! think G-Biosciences www.gbiosciences.com

Dot Blot Analysis. Teacher s Guidebook. (Cat. # BE 502) think proteins! think G-Biosciences www.gbiosciences.com PR110 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Dot Blot Analysis Teacher s Guidebook (Cat. # BE 502) think proteins! think G-Biosciences

More information

Short Communication. Production of Monoclonal and Polyclonal Antibodies against Human Alphafetoprotein, a Hepatocellular Tumor Marker ABSTRACT

Short Communication. Production of Monoclonal and Polyclonal Antibodies against Human Alphafetoprotein, a Hepatocellular Tumor Marker ABSTRACT HYBRIDOMA AND HYBRIDOMICS Volume 21, Number 4, 2002 Mary Ann Liebert, Inc. Short Communication Production of Monoclonal and Polyclonal Antibodies against Human Alphafetoprotein, a Hepatocellular Tumor

More information

P R O D U C T S CATALOG 2011-2012

P R O D U C T S CATALOG 2011-2012 P R O D U C T S CATALOG 2011-2012 INSTITUTE FOR THE APPLICATION OF NUCLEAR ENERGY - INEP Banatska 31b 11080 Zemun Belgrade Serbia Tel: (+381 11) 2619 252, 2618 696, 2199 949 Fax: (+381 11) 2618 724 www.inep.co.rs

More information

CUSTOM ANTIBODY SERVICES & PRODUCTS

CUSTOM ANTIBODY SERVICES & PRODUCTS CUSTOM ANTIBODY SERVICES & PRODUCTS POLYCLONAL SERVICES MONOCLONAL SERVICES ANTIBODY SUPPORT SERVICES ANTIGEN SUBMISSION ANTIBODY PROJECT INITIATION CUSTOM ANTIBODY SERVICES & PRODUCTS CUSTOM ANTIBODY

More information

Mouse Typer Sub-Isotyping Kit Instruction Manual

Mouse Typer Sub-Isotyping Kit Instruction Manual Mouse Typer Sub-Isotyping Kit Instruction Manual Protocol for Mouse Typer Sub-Isotyping Kit (Catalog Number 172-2051) and Mouse Typer Sub Isotyping Panel (Catalog Number 172-2055) For Technical Service

More information

POLYCLONAL ANTIBODY DEVELOPMENT

POLYCLONAL ANTIBODY DEVELOPMENT POLYCLONAL ANTIBODY DEVELOPMENT Abcore is committed to excellence, and to responding to the changing needs of our customers. We have developed polyclonal antibodies for biotechnology and pharmaceutical

More information

The production of monoclonal antibodies using the hybridoma technology

The production of monoclonal antibodies using the hybridoma technology The production of monoclonal antibodies using the hybridoma technology Sbonelo Hadebe 211554629 October 16, 2015 Contents 1 Introduction 1 2 Theory 2 2.1 Monoclonal antibodies...................... 2 2.2

More information

JIANGSU CARTMAY INDUSTRIAL CO.,LTD www.labfurniture.asia mail: info@labfurniture.asia

JIANGSU CARTMAY INDUSTRIAL CO.,LTD www.labfurniture.asia mail: info@labfurniture.asia The basic layout, the main functions and instrumentation concept of micro Inspection Division laboratory, 1, Virology Laboratory 1. Functions: for the city to monitor the prevalence of HIV disease, dealing

More information

Immunization, immune sera and the production of antibodies

Immunization, immune sera and the production of antibodies Immunization, immune sera and the production of antibodies WOLF D. KUHLMANN, M.D. Division of Radiooncology, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany The overall characteristic of antibody

More information

Davids Biotechnologie GmbH Custom Antibodies: Introduction How to order Custom Antibodies

Davids Biotechnologie GmbH Custom Antibodies: Introduction How to order Custom Antibodies DAVIDS BIOTECHNOLOGIE GMBH CATALOG 2016 Custom Antibodies 2 Davids Biotechnologie GmbH Custom Antibodies: Introduction How to order Custom Antibodies Antigen Production 4 Suitable Antigens Peptides As

More information

Some Immunological Test. Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras

Some Immunological Test. Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras Some Immunological Test Presented by Alaa Faeiz Ashwaaq Dyaa Aseel Abd AL-Razaq Supervised by D.Feras Alaa Faeiz Antigen -Antibody Reactions. Antigen antibody reactions are performed to determine the presence

More information

Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan

Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan 489 18 Production of Monoclonal Catalytic Antibodies: Principles and Practice Diane Kubitz, Ehud Keinan 18.1 Introduction Antibody catalysis is an experimental science. Although success in this field is

More information