High-performance Image Analysis for Digital Pathology
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- Ashlyn Harrison
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1 TRAI N I NG AND SU PP O RT Besides on-site installation and training, Visiopharm offers on-line support, Developing image analysis protocols allowing our experts to assist in developing Standard Operating Procedures, analysis protocols, and guide users every step of the way. HARDWARE CO MPATI B I LIT Y V ISI O M O RPH D P IS A SO PH IS TI C AT E D Y E T I NT U ITI V E I NS TRUMENT FO R D E V ELO PI NG A DVAN CED I M AG E A N A LYSIS PROTO CO L S. High-performance Image Analysis for Digital Pathology Visiopharm provides systems fully configured with all hardware components, but retrofitting is also an option. VisiomorphDPTM is fully compatible with all the leading hardware brands. The expert user is provided with a logical set of steps that guides the development of image analysis protocols. When an image analysis protocol has been defined for a given application, high volumes of image data can be processed with minimal user- Microscopes: Leica, Nikon, Olympus, Zeiss interaction and under highly standardized conditions. Confocal microscopes: Leica, Olympus, and Zeiss VisiomorphDP by Visiopharm Whole Slide file formats: 3DHISTECH, Aperio, Hamamatsu, Leica, Olympus 1. Definition of taxonomy: The VisiomorphDP label tool 4. Segmenting: Selection and definition of a decision rule can be used for naming relevant structural content to be combining the defined image features and training data, recognized. based on which images are segmented into their relevant Stages: Ludl, Märzhäuser, and Prior components. Slide loaders: Ludl Camera: Basler, Hamamatsu, Leica, Olympus, 2. Training: Teach-by-example allows the user to train the system by painting examples of the defined structures 5. Post processing: This allows for further refinement of the and BMP segmentation result based on morphological and contex- in the image. 3. Pre-processing: This is a set of operations allowing for tual information. 6. Calculating results: Specification of end-points based on age segmentation, combining spectral, local spatial, and the segmented image, the original image, derived feature local morphological information. images, and combinations of other end-points. Train by example Pre-process Segment image Post process and QImaging PC: enhancement of relevant image structures for use in im- Define Taxonomy Static image file formats: Most common file formats, including TIFF, JPEG All Major brands with Microsoft Windows XP, Vista and 7 Calculate results Running protocols and reviewing results VISI O M O RPH D P IS AU TO M ATI NG, S T R E A MLI N I NG, AND S TANDAR D IzI NG E SS ENTI AL S TEP S TO C RE AT E k N OW L E D G E I N D I G ITA L PATH O LO G Y. With the concept of user-defined image analysis protocols, the learning curve for non-expert operators is greatly reduced. Detailed knowledge of the specific analysis is not required to execute the protocols. M O RE I NFO RMATI O N 1. Load protocol: Simple access to all application specific 3. Review results: Sophisticated tools allow operators and image analysis protocols allows the non-expert user to application specialists to review the results in a way which is perform image analysis procedures without detailed easy, fast and convenient even for large and complex stud- Visiopharm A/S knowledge of the design decisions implemented in the ies. Data are presented in a spreadsheet format. By clicking protocols by the expert user. on any entry, pertinent image data, meta-data, and end- Agern Allé 3 DK-2970 Hørsholm 2. Batch process: By a few clicks the non-expert user can run points are presented to the operator for easy reviewing. visopharm.com the defined image analysis procedure on individual or VisiomorphDP allows for human intervention at any point Phone: batches of images in the database. Generated data are in time, enabling the operator to challenge and change contact@visiopharm.com automatically stored in the database. computed results. An electronic audit trail makes it possible Batch process Review Yellow. Printed in Denmark by Cool Grey to track changes and actions performed by the user. Load protocol Please contact: V ISI O M O R PH D P TM IS A V E R SATI L E A N D D E D IC ATE D TO O L FO R CH A R AC TE R Iz ATI O N A N D q UA NTI F IC ATI O N O F S TRU C TU R E S A N D M A R k E R S I N D I GITA L I M AGE S O F TISSUE SEC TI O NS A N D C Y TO LO G Y SL I D E S. Visiopharm products are not for clinical diagnostic use.
2 Image analysis software for knowledge creation Application examples Fully integrated data management V ISI O M O RPH D P PROV I D E S A S E T O F H I G HLY SPEC I ALIz ED TO O L S FAC I LITATI NG k E Y A SPEC T S VISI O M O RPHD P E N AB LE S q UANTITATIVE AN ALYSIS O F WH O LE SLI D E I MAGE S R E SE A RCH I N VO LV I N G I M AGE S A N D I M AGE A N A LYSIS O F TE N GE N E R ATE S DATA WITH O F WO R ki N G WITH q UA NTITATI V E I M AG E AN ALYSIS AND DATA MAN AGEMENT. FRO M BOTH B R I GHT FI E LD AND MU LTISPEC TRAL FLU O RE SCE NCE. CO M PL E x R E L ATI O NSH I P S. The image analysis tools are optimized for the specific The roles of expert and operator are supported by two dif- challenges in whole slide images, and simplify the unam- ferent user interface modes. In Design Mode, the expert biguous expression of histological end-points in terms of user can manipulate all the details of a given analysis proto- spectral, spatial, morphological, and contextual image col to match the exact requirements of a given application. properties. In Process Mode, a simple user interface gives access to all application specific image analysis protocols in the data The knowledge and decisions of experts are implemented library. This allows any operator to perform image analysis electronically as image analysis protocols. These electronic procedures without detailed knowledge of the design deci- protocols can easily be loaded and executed by any autho- sions implemented in the protocols by the domain expert. Images and measurement data are automatically added to the integrated database in an organized folder-structure as seen in the left part of the image. This allows the user to collect and search information about individual or several images. Information and endpoints related to the specific entry (image) is shown on the right hand side. This includes a thumbnail image, image file root path, and any endpoints; in this case, the number of red and green signals and the amplification (number of red signals divided by number of green signals). rized operator, either on individual regions of interest (ROIs), entire whole slide images, or batches of images. Because data management is fully integrated in the work- The VisiomorphDP system database provides efficient flow, it is easy to maintain data integrity also with large tools for reviewing and editing data, while maintaining Example: Co-localization analysis data sets and complex relationships. Captured images an electronic audit trail of all actions. Additional function- In co-localization two (or more) different fluorescent markers present a spatial proximity indicating a biological interac- and computed end-points are stored effortlessly in the ality can be included to achieve regulatory compliance in tion or relationship between pairs (or groups) of bio-molecules. To accurately describe this relationship or interaction, database, which electronically links all image data, meta- regards to 21 CFR Part 11. Exporting computed end-points the amount of co-localized signals must be quantified. The available pre-processing steps in VisiomorphDPTM allows for data, and computed end-points. The database is custom- for further analysis is made simple, as a single click allows user-defined settings of thresholds on the amount of red and green that should be present at the same time to constitute izable and new fields in the database can be created to for exporting data from an entire study to an Excel spread- colocalized signals (middle image), as well as detecting the individual red/green only signals. accommodate additional study information. Advanced sheet. The database is based on MS SQL Server Express, search capabilities make it possible to mine data for criti- making it easy to access data by other applications, such cal information about disease progression as well as test as Laboratory Information Management Systems. article-related toxicity or efficacy. Include spectral and morphological information in your image analysis protocol to retrieve meaningful histomorphometrical measurements. By the use of contextual post processing steps, VisiomorphDP allows you to modify the segmentation results to obtain more specific identification of even complex anatomical structures. Define relevant output and receive a full protocol for automated measurement of e.g. the area of transected blood vessels. Extending the VisiomorphDP system VisiomorphDPTM enables the user to work with whole slide file formats from most scanner vendors, and the analysis I NTEGR ATE WITH A N I M AGE SE RV E R FO R A CO M PL E TE LY I NTEGR ATE D D I GITA L PATH O LO G Y protocols can be executed at the exact magnification WO R k F LOW O R U P GR A D E TO A S TATE- O F-TH E A RT WH O L E SL I D E S TE R EO LO G Y S YS TE M. required for quantification, as defined by the user. This can save significant amounts of time, when quantification is VisiomorphDP and all other software packages from of TMAs and other array configurations is facilitated while Visiopharm can be used with all major slide scanner complete traceability of the data is maintained. In addi- scanning. E.g. analyzing at 10X instead of 20X is 4 times Example: Gene aberration analysis by FISH /CISH formats and many slide databases. This facilitates cross- tion, the system can be extended with newcast, which faster. Aberrations in the copy numbers of marker genes encoding receptors, such as HER2, or enzymes, such as topoisomerase platform collaboration and gives the freedom to adopt implements all essential stereological probes and other IIa (TOP2A), can be determined by fluorescence or bright field in situ hybridization (FISH/BRISH). When used for studying the scanner platform of choice. These integrations with tools for obtaining statistically unbiased estimates of e.g., To support a more intuitive setup of analysis protocols, cancer tissue, each copy of marker gene is typically labeled by one color (e.g. red fluorescent signals in FISH), whereas each the slide databases are an important step towards a more number, length, area, and volume. Upgrades are available color de-convolution is available to let the user work in copy of an internal reference gene is labeled by another color (e.g. green fluorescent signals). By using VisiomorphDP, the optimal digital pathology workflow. TissuemorphDPTM for newcast (AutoDisector and Proportionator ) to manual counting procedures for determination of signal ratios can be substituted by automated detection and discrimina- can be added to the platform to have a specialized tool further automate and dramatically reduce the workload tion. The use of proprietary filters in the algorithms ensures spatial recognition and color determination of the signals, and for nuclei, membrane and cytoplasmic quantification. in workflows for whole slide stereology. the ratio of colored signals is reported. Further, with the ArrayImager add-in module the analysis possible at a magnification lower than what was used for true stain space. A list of predefined stains is available or user-defined stains can be created based on the images at hand. DAB Color-deconvolution: Example of how the DAB stain is visually presented in VisiomorphDP. Standard stain de-convolutions are included in the software for a number of typical chromagens, and the user can supplement these by creating their own, if required.
3 Image analysis software for knowledge creation Application examples Fully integrated data management V ISI O M O RPH D P PROV I D E S A S E T O F H I G HLY SPEC I ALIz ED TO O L S FAC I LITATI NG k E Y A SPEC T S VISI O M O RPHD P E N AB LE S q UANTITATIVE AN ALYSIS O F WH O LE SLI D E I MAGE S R E SE A RCH I N VO LV I N G I M AGE S A N D I M AGE A N A LYSIS O F TE N GE N E R ATE S DATA WITH O F WO R ki N G WITH q UA NTITATI V E I M AG E AN ALYSIS AND DATA MAN AGEMENT. FRO M BOTH B R I GHT FI E LD AND MU LTISPEC TRAL FLU O RE SCE NCE. CO M PL E x R E L ATI O NSH I P S. The image analysis tools are optimized for the specific The roles of expert and operator are supported by two dif- challenges in whole slide images, and simplify the unam- ferent user interface modes. In Design Mode, the expert biguous expression of histological end-points in terms of user can manipulate all the details of a given analysis proto- spectral, spatial, morphological, and contextual image col to match the exact requirements of a given application. properties. In Process Mode, a simple user interface gives access to all application specific image analysis protocols in the data The knowledge and decisions of experts are implemented library. This allows any operator to perform image analysis electronically as image analysis protocols. These electronic procedures without detailed knowledge of the design deci- protocols can easily be loaded and executed by any autho- sions implemented in the protocols by the domain expert. Images and measurement data are automatically added to the integrated database in an organized folder-structure as seen in the left part of the image. This allows the user to collect and search information about individual or several images. Information and endpoints related to the specific entry (image) is shown on the right hand side. This includes a thumbnail image, image file root path, and any endpoints; in this case, the number of red and green signals and the amplification (number of red signals divided by number of green signals). rized operator, either on individual regions of interest (ROIs), entire whole slide images, or batches of images. Because data management is fully integrated in the work- The VisiomorphDP system database provides efficient flow, it is easy to maintain data integrity also with large tools for reviewing and editing data, while maintaining Example: Co-localization analysis data sets and complex relationships. Captured images an electronic audit trail of all actions. Additional function- In co-localization two (or more) different fluorescent markers present a spatial proximity indicating a biological interac- and computed end-points are stored effortlessly in the ality can be included to achieve regulatory compliance in tion or relationship between pairs (or groups) of bio-molecules. To accurately describe this relationship or interaction, database, which electronically links all image data, meta- regards to 21 CFR Part 11. Exporting computed end-points the amount of co-localized signals must be quantified. The available pre-processing steps in VisiomorphDPTM allows for data, and computed end-points. The database is custom- for further analysis is made simple, as a single click allows user-defined settings of thresholds on the amount of red and green that should be present at the same time to constitute izable and new fields in the database can be created to for exporting data from an entire study to an Excel spread- colocalized signals (middle image), as well as detecting the individual red/green only signals. accommodate additional study information. Advanced sheet. The database is based on MS SQL Server Express, search capabilities make it possible to mine data for criti- making it easy to access data by other applications, such cal information about disease progression as well as test as Laboratory Information Management Systems. article-related toxicity or efficacy. Include spectral and morphological information in your image analysis protocol to retrieve meaningful histomorphometrical measurements. By the use of contextual post processing steps, VisiomorphDP allows you to modify the segmentation results to obtain more specific identification of even complex anatomical structures. Define relevant output and receive a full protocol for automated measurement of e.g. the area of transected blood vessels. Extending the VisiomorphDP system VisiomorphDPTM enables the user to work with whole slide file formats from most scanner vendors, and the analysis I NTEGR ATE WITH A N I M AGE SE RV E R FO R A CO M PL E TE LY I NTEGR ATE D D I GITA L PATH O LO G Y protocols can be executed at the exact magnification WO R k F LOW O R U P GR A D E TO A S TATE- O F-TH E A RT WH O L E SL I D E S TE R EO LO G Y S YS TE M. required for quantification, as defined by the user. This can save significant amounts of time, when quantification is VisiomorphDP and all other software packages from of TMAs and other array configurations is facilitated while Visiopharm can be used with all major slide scanner complete traceability of the data is maintained. In addi- scanning. E.g. analyzing at 10X instead of 20X is 4 times Example: Gene aberration analysis by FISH /CISH formats and many slide databases. This facilitates cross- tion, the system can be extended with newcast, which faster. Aberrations in the copy numbers of marker genes encoding receptors, such as HER2, or enzymes, such as topoisomerase platform collaboration and gives the freedom to adopt implements all essential stereological probes and other IIa (TOP2A), can be determined by fluorescence or bright field in situ hybridization (FISH/BRISH). When used for studying the scanner platform of choice. These integrations with tools for obtaining statistically unbiased estimates of e.g., To support a more intuitive setup of analysis protocols, cancer tissue, each copy of marker gene is typically labeled by one color (e.g. red fluorescent signals in FISH), whereas each the slide databases are an important step towards a more number, length, area, and volume. Upgrades are available color de-convolution is available to let the user work in copy of an internal reference gene is labeled by another color (e.g. green fluorescent signals). By using VisiomorphDP, the optimal digital pathology workflow. TissuemorphDPTM for newcast (AutoDisector and Proportionator ) to manual counting procedures for determination of signal ratios can be substituted by automated detection and discrimina- can be added to the platform to have a specialized tool further automate and dramatically reduce the workload tion. The use of proprietary filters in the algorithms ensures spatial recognition and color determination of the signals, and for nuclei, membrane and cytoplasmic quantification. in workflows for whole slide stereology. the ratio of colored signals is reported. Further, with the ArrayImager add-in module the analysis possible at a magnification lower than what was used for true stain space. A list of predefined stains is available or user-defined stains can be created based on the images at hand. DAB Color-deconvolution: Example of how the DAB stain is visually presented in VisiomorphDP. Standard stain de-convolutions are included in the software for a number of typical chromagens, and the user can supplement these by creating their own, if required.
4 T h e V i s i o p h a r m I n t e g r ato r S y s t e m Image analysis software for knowledge creation Application examples Fully integrated data management Visi o m o rph D P provides a se t o f highly specialized too l s faci litati ng k e y a spec ts Visi o m o rphd P en ab les q uantitative an alysis o f wh o le slide i m ages Rese a rch i n vo lvi n g i m ages a n d i m age a n a lysis o f te n ge n e r ates data with o f wo rk i ng w ith q ua ntitative i mage an alysis and data man agement. fro m both b r ight field and mu ltispec tral flu o rescence. co m pl e x r e l ati o nshi p s. The image analysis tools are optimized for the specific The roles of expert and operator are supported by two dif- challenges in whole slide images, and simplify the unam- ferent user interface modes. In Design Mode, the expert biguous expression of histological end-points in terms of user can manipulate all the details of a given analysis proto- spectral, spatial, morphological, and contextual image col to match the exact requirements of a given application. properties. In Process Mode, a simple user interface gives access to all application specific image analysis protocols in the data The knowledge and decisions of experts are implemented library. This allows any operator to perform image analysis electronically as image analysis protocols. These electronic procedures without detailed knowledge of the design deci- protocols can easily be loaded and executed by any autho- sions implemented in the protocols by the domain expert. Images and measurement data are automatically added to the integrated database in an organized folder-structure as seen in the left part of the image. This allows the user to collect and search information about individual or several images. Information and endpoints related to the specific entry (image) is shown on the right hand side. This includes a thumbnail image, image file root path, and any endpoints; in this case, the number of red and green signals and the amplification (number of red signals divided by number of green signals). rized operator, either on individual regions of interest (ROIs), entire whole slide images, or batches of images. Because data management is fully integrated in the work- The VisiomorphDP system database provides efficient flow, it is easy to maintain data integrity also with large tools for reviewing and editing data, while maintaining Example: Co-localization analysis data sets and complex relationships. Captured images an electronic audit trail of all actions. Additional function- In co-localization two (or more) different fluorescent markers present a spatial proximity indicating a biological interac- and computed end-points are stored effortlessly in the ality can be included to achieve regulatory compliance in tion or relationship between pairs (or groups) of bio-molecules. To accurately describe this relationship or interaction, database, which electronically links all image data, meta- regards to 21 CFR Part 11. Exporting computed end-points the amount of co-localized signals must be quantified. The available pre-processing steps in VisiomorphDPTM allows for data, and computed end-points. The database is custom- for further analysis is made simple, as a single click allows user-defined settings of thresholds on the amount of red and green that should be present at the same time to constitute izable and new fields in the database can be created to for exporting data from an entire study to an Excel spread- colocalized signals (middle image), as well as detecting the individual red/green only signals. accommodate additional study information. Advanced sheet. The database is based on MS SQL Server Express, search capabilities make it possible to mine data for criti- making it easy to access data by other applications, such cal information about disease progression as well as test as Laboratory Information Management Systems. article-related toxicity or efficacy. Include spectral and morphological information in your image analysis protocol to retrieve meaningful histomorphometrical measurements. By the use of contextual post processing steps, VisiomorphDP allows you to modify the segmentation results to obtain more specific identification of even complex anatomical structures. Define relevant output and receive a full protocol for automated measurement of e.g. the area of transected blood vessels. Extending the VisiomorphDP system VisiomorphDPTM enables the user to work with whole slide file formats from most scanner vendors, and the analysis I nteg r ate with a n i m age serve r fo r a co m pl e te ly i nteg r ated digita l path o lo g y protocols can be executed at the exact magnification wo r kf low o r u p g r a de to a s tate- o f-the a rt wh o l e s l ide s te r eo lo g y s ys te m. required for quantification, as defined by the user. This can save significant amounts of time, when quantification is VisiomorphDP and all other software packages from of TMAs and other array configurations is facilitated while Visiopharm can be used with all major slide scanner complete traceability of the data is maintained. In addi- scanning. E.g. analyzing at 10X instead of 20X is 4 times Example: Gene aberration analysis by FISH /CISH formats and many slide databases. This facilitates cross- tion, the system can be extended with newcast, which faster. Aberrations in the copy numbers of marker genes encoding receptors, such as HER2, or enzymes, such as topoisomerase platform collaboration and gives the freedom to adopt implements all essential stereological probes and other IIa (TOP2A), can be determined by fluorescence or bright field in situ hybridization (FISH/BRISH). When used for studying the scanner platform of choice. These integrations with tools for obtaining statistically unbiased estimates of e.g., To support a more intuitive setup of analysis protocols, cancer tissue, each copy of marker gene is typically labeled by one color (e.g. red fluorescent signals in FISH), whereas each the slide databases are an important step towards a more number, length, area, and volume. Upgrades are available color de-convolution is available to let the user work in copy of an internal reference gene is labeled by another color (e.g. green fluorescent signals). By using VisiomorphDP, the optimal digital pathology workflow. TissuemorphDPTM for newcast (AutoDisector and Proportionator ) to manual counting procedures for determination of signal ratios can be substituted by automated detection and discrimina- can be added to the platform to have a specialized tool further automate and dramatically reduce the workload tion. The use of proprietary filters in the algorithms ensures spatial recognition and color determination of the signals, and for nuclei, membrane and cytoplasmic quantification. in workflows for whole slide stereology. the ratio of colored signals is reported. Further, with the ArrayImager add-in module the analysis possible at a magnification lower than what was used for true stain space. A list of predefined stains is available or user-defined stains can be created based on the images at hand Visiomorph v03.indd 2 DAB Color-deconvolution: Example of how the DAB stain is visually presented in VisiomorphDP. Standard stain de-convolutions are included in the software for a number of typical chromagens, and the user can supplement these by creating their own, if required. 1/6/12 10:53 AM
5 TRAI N I NG AND SU PP O RT Besides on-site installation and training, Visiopharm offers on-line support, Developing image analysis protocols allowing our experts to assist in developing Standard Operating Procedures, analysis protocols, and guide users every step of the way. HARDWARE CO MPATI B I LIT Y V ISI O M O RPH D P IS A SO PH IS TI C AT E D Y E T I NT U ITI V E I NS TRUMENT FO R D E V ELO PI NG A DVAN CED I M AG E A N A LYSIS PROTO CO L S. High-performance Image Analysis for Digital Pathology Visiopharm provides systems fully configured with all hardware components, but retrofitting is also an option. VisiomorphDPTM is fully compatible with all the leading hardware brands. The expert user is provided with a logical set of steps that guides the development of image analysis protocols. When an image analysis protocol has been defined for a given application, high volumes of image data can be processed with minimal user- Microscopes: Leica, Nikon, Olympus, Zeiss interaction and under highly standardized conditions. Confocal microscopes: Leica, Olympus, and Zeiss VisiomorphDP by Visiopharm Whole Slide file formats: 3DHISTECH, Aperio, Hamamatsu, Leica, Olympus 1. Definition of taxonomy: The VisiomorphDP label tool 4. Segmenting: Selection and definition of a decision rule can be used for naming relevant structural content to be combining the defined image features and training data, recognized. based on which images are segmented into their relevant Stages: Ludl, Märzhäuser, and Prior components. Slide loaders: Ludl Camera: Basler, Hamamatsu, Leica, Olympus, 2. Training: Teach-by-example allows the user to train the system by painting examples of the defined structures 5. Post processing: This allows for further refinement of the and BMP segmentation result based on morphological and contex- in the image. 3. Pre-processing: This is a set of operations allowing for tual information. 6. Calculating results: Specification of end-points based on age segmentation, combining spectral, local spatial, and the segmented image, the original image, derived feature local morphological information. images, and combinations of other end-points. Train by example Pre-process Segment image Post process and QImaging PC: enhancement of relevant image structures for use in im- Define Taxonomy Static image file formats: Most common file formats, including TIFF, JPEG All Major brands with Microsoft Windows XP, Vista and 7 Calculate results Running protocols and reviewing results VISI O M O RPH D P IS AU TO M ATI NG, S T R E A MLI N I NG, AND S TANDAR D IzI NG E SS ENTI AL S TEP S TO C RE AT E k N OW L E D G E I N D I G ITA L PATH O LO G Y. With the concept of user-defined image analysis protocols, the learning curve for non-expert operators is greatly reduced. Detailed knowledge of the specific analysis is not required to execute the protocols. M O RE I NFO RMATI O N 1. Load protocol: Simple access to all application specific 3. Review results: Sophisticated tools allow operators and image analysis protocols allows the non-expert user to application specialists to review the results in a way which is perform image analysis procedures without detailed easy, fast and convenient even for large and complex stud- Visiopharm A/S knowledge of the design decisions implemented in the ies. Data are presented in a spreadsheet format. By clicking protocols by the expert user. on any entry, pertinent image data, meta-data, and end- Agern Allé 3 DK-2970 Hørsholm 2. Batch process: By a few clicks the non-expert user can run points are presented to the operator for easy reviewing. visopharm.com the defined image analysis procedure on individual or VisiomorphDP allows for human intervention at any point Phone: batches of images in the database. Generated data are in time, enabling the operator to challenge and change contact@visiopharm.com automatically stored in the database. computed results. An electronic audit trail makes it possible Batch process Review Yellow. Printed in Denmark by Cool Grey to track changes and actions performed by the user. Load protocol Please contact: V ISI O M O R PH D P TM IS A V E R SATI L E A N D D E D IC ATE D TO O L FO R CH A R AC TE R Iz ATI O N A N D q UA NTI F IC ATI O N O F S TRU C TU R E S A N D M A R k E R S I N D I GITA L I M AGE S O F TISSUE SEC TI O NS A N D C Y TO LO G Y SL I D E S. Visiopharm products are not for clinical diagnostic use.
6 TRAI N I NG AND SU PP O RT Besides on-site installation and training, Visiopharm offers on-line support, Developing image analysis protocols allowing our experts to assist in developing Standard Operating Procedures, analysis protocols, and guide users every step of the way. HARDWARE CO MPATI B I LIT Y V ISI O M O RPH D P IS A SO PH IS TI C AT E D Y E T I NT U ITI V E I NS TRUMENT FO R D E V ELO PI NG A DVAN CED I M AG E A N A LYSIS PROTO CO L S. High-performance Image Analysis for Digital Pathology Visiopharm provides systems fully configured with all hardware components, but retrofitting is also an option. VisiomorphDPTM is fully compatible with all the leading hardware brands. The expert user is provided with a logical set of steps that guides the development of image analysis protocols. When an image analysis protocol has been defined for a given application, high volumes of image data can be processed with minimal user- Microscopes: Leica, Nikon, Olympus, Zeiss interaction and under highly standardized conditions. Confocal microscopes: Leica, Olympus, and Zeiss VisiomorphDP by Visiopharm Whole Slide file formats: 3DHISTECH, Aperio, Hamamatsu, Leica, Olympus 1. Definition of taxonomy: The VisiomorphDP label tool 4. Segmenting: Selection and definition of a decision rule can be used for naming relevant structural content to be combining the defined image features and training data, recognized. based on which images are segmented into their relevant Stages: Ludl, Märzhäuser, and Prior components. Slide loaders: Ludl Camera: Basler, Hamamatsu, Leica, Olympus, 2. Training: Teach-by-example allows the user to train the system by painting examples of the defined structures 5. Post processing: This allows for further refinement of the and BMP segmentation result based on morphological and contex- in the image. 3. Pre-processing: This is a set of operations allowing for tual information. 6. Calculating results: Specification of end-points based on age segmentation, combining spectral, local spatial, and the segmented image, the original image, derived feature local morphological information. images, and combinations of other end-points. Train by example Pre-process Segment image Post process and QImaging PC: enhancement of relevant image structures for use in im- Define Taxonomy Static image file formats: Most common file formats, including TIFF, JPEG All Major brands with Microsoft Windows XP, Vista and 7 Calculate results Running protocols and reviewing results VISI O M O RPH D P IS AU TO M ATI NG, S T R E A MLI N I NG, AND S TANDAR D IzI NG E SS ENTI AL S TEP S TO C RE AT E k N OW L E D G E I N D I G ITA L PATH O LO G Y. With the concept of user-defined image analysis protocols, the learning curve for non-expert operators is greatly reduced. Detailed knowledge of the specific analysis is not required to execute the protocols. M O RE I NFO RMATI O N 1. Load protocol: Simple access to all application specific 3. Review results: Sophisticated tools allow operators and image analysis protocols allows the non-expert user to application specialists to review the results in a way which is perform image analysis procedures without detailed easy, fast and convenient even for large and complex stud- Visiopharm A/S knowledge of the design decisions implemented in the ies. Data are presented in a spreadsheet format. By clicking protocols by the expert user. on any entry, pertinent image data, meta-data, and end- Agern Allé 3 DK-2970 Hørsholm 2. Batch process: By a few clicks the non-expert user can run points are presented to the operator for easy reviewing. visopharm.com the defined image analysis procedure on individual or VisiomorphDP allows for human intervention at any point Phone: batches of images in the database. Generated data are in time, enabling the operator to challenge and change contact@visiopharm.com automatically stored in the database. computed results. An electronic audit trail makes it possible Batch process Review Yellow. Printed in Denmark by Cool Grey to track changes and actions performed by the user. Load protocol Please contact: V ISI O M O R PH D P TM IS A V E R SATI L E A N D D E D IC ATE D TO O L FO R CH A R AC TE R Iz ATI O N A N D q UA NTI F IC ATI O N O F S TRU C TU R E S A N D M A R k E R S I N D I GITA L I M AGE S O F TISSUE SEC TI O NS A N D C Y TO LO G Y SL I D E S. Visiopharm products are not for clinical diagnostic use.
MI Software. Innovation with Integrity. High Performance Image Analysis and Publication Tools. Preclinical Imaging
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