Servicio de Salud de Castilla-La Mancha
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1 Servicio de Salud de Castilla-La Mancha Telepathology network of Castilla-La Mancha MARCIAL GARCÍA ROJO Chief. Pathology Department Hospital General de Ciudad Real Servicio de Anatomía Patológica miércoles, 23 de junio de 2010
2 Servicio de Salud de Castilla-La Mancha 23/06/ Introduction New technologies in Pathology (information systems, digital photography, digital slides) allow a more efficient work in Pathology Departments. There is a growing automation in the specialty. Digital Image of good quality allow diagnoses similar to the use of conventional microscopes In 2004, the Public Health Service of Castilla-La Mancha (SESCAM) created an Pathology Information Technology Innovation Centre.
3 Current status of slide scanning Advantages and disadvantages vs. traditional microscopy In clinical practice (63% of digital pathology users) In education (72%) Servicio de Salud de Castilla-La Mancha 23/06/2010 3
4 Servicio de Salud de Castilla-La Mancha 23/06/ Drawbacks of glass slides Conventional glass slides are fragile They are not permanent: immunofluorescence, crystal studies in joint effusions In cytology, distributing copies of the same specimen is not possible
5 Servicio de Salud de Castilla-La Mancha 23/06/ Advantages of digital slides Tissue area: generally 25 x 20 mm Low magnifications with extraordinary quality Dynamic map of the slide Optimization of network bandwidth: high resolutions areas are sent of request area only. Record and reproduce slide navigation (traceability of diagnosis) Images are permanently stored without any loss of quality Annotations are also saved permanently.
6 A digital ( virtual ) slide is a real slide, and physically it is A folder with a set of files. Generally JPEG files, and a coordinates file used to reconstruct the complete slide. Advantages: Flexibility (one folder per Z plane, or per magnification) Disadvantages: Difficult to copy and distribute No real standard. Used by Zoomify of similar (MS Silverlight) DICOM A single file (SVS, JPEG2000, Philips ) multirresolution, containing also all information associated with the image Advantages: Easy to distribute. JPEG2000 is a standard Disadvantages: Performance issues in JPEG2000 Servicio de Salud de Castilla-La Mancha 23/06/2010 6
7 Servicio de Salud de Castilla-La Mancha 23/06/ DICOM Sup145 multi-frame tiling Use multi-frame image objects (not object per tile) Thumbnail Image Single frame image Intermediate Image Tiles Multi-frame image (single object) Baseline Image Tiles Multi-frame image (single object) may include multiple Z-planes, color planes In 1 or more DICOM Series
8 DICOM. Supplement 122. Single file: JPEG2000/JPIP Servicio de Salud de Castilla-La Mancha 23/06/2010 8
9 When no compression is applied, large storage amount is needed Servicio de Salud de Castilla-La Mancha 23/06/ Magnification Numerical Aperture Optical Resolution (microns) Minimum store needed to save all those pixels (24-bits colour) 4x 0,2 1,7 2,08 GB 10x 0,45 0,75 11 GB 20x 0,5 0,67 13 GB 40x 0,75 0,45 30 GB Many image analysis algorythms can be applied using compressed images
10 Servicio de Salud de Castilla-La Mancha 23/06/ Clinical practice European network: Action IC0604: Telepathology Network (EURO-TELEPATH) Telepathology in Castilla-La Mancha. Serendipia project in SESCAM Main objective: Creating a professional communication tool connecting all Pathology Departments using a web platform and all needed devices or systems. Following existing standards
11 Servicio de Salud de Castilla-La Mancha 23/06/ Serendipia project
12 Servicio de Salud de Castilla-La Mancha 23/06/ Integration with Pathology Information system in a web environment View gross pictures and digital slides
13 Servicio de Salud de Castilla-La Mancha 23/06/ Integration of all kind of images
14 Image Analysis Servicio de Salud de Castilla-La Mancha 23/06/
15 Measure, quantify and analyze Tools to measure: Melanomas: Breslow thickness Sentinel lymph nodes: Localize and distinguish between isolated cells and micrometastasis Image analysis (immunohistochemistry) algorithms: Her2, oestrogen and progesterone receptors (62% users of digital pathology apply quantitative immunohistochemistry for HER2 scoring) Detection of areas of interest (cytology) Servicio de Salud de Castilla-La Mancha 23/06/
16 Servicio de Salud de Castilla-La Mancha 23/06/ Viewer
17 10B0XXXX-3 HE_02.vsi Olympus Servicio de Salud dotslide de Castilla-La Mancha 17
18 Aperio Scanscope Servicio de Salud de Castilla-La Mancha 18 HER2\Caso1.svs
19 Second opinion and teleconsultation Servicio de Salud de Castilla-La Mancha 23/06/
20 Education Servicio de Salud de Castilla-La Mancha 23/06/
21 Servicio de Salud de Castilla-La Mancha 23/06/ Library of interesting virtual slides o Biblioteca pedagógica
22 Main problems with existing scanners Slow 40x scanning process (up to 90 min / cytology specimen) Out of focus areas Stitching artefacts Multiple Z planes may be necessary File formats are not standards Integration with other systems Costs Servicio de Salud de Castilla-La Mancha 23/06/
23 Main criteria in scanner purchasing Image quality: Avoid stitching artefacts and outof-focus areas Scanning speed at 40x Image managing Price Integration capabilities (LIS, PACS): Standards Maintenance and support Upgrading policy: software updates and access to new versions of the scanner Servicio de Salud de Castilla-La Mancha 23/06/
24 Servicio de Salud de Castilla-La Mancha 23/06/ Automated image analysis IHQ quantification CISH/SISH analysis Fluorescence (FISH, Skin, Kidney) TMA FDA Clearance Her2/neu, Ostrogen and Progestorone Receptors Open solutions: Image analysis software should be able to read multiple digital slide file formats Image analysis results should be integrated with LIS reports Annotation should be interchangeable betweeb diferent vendors (no standard!) Integration with cytology solutions: Hologic ThinPrep Imaging System, Civagen FocalPoint
25 Scanning solutions Spectrum of different scanner options (autoloader, oil, fluorescence): 3DHistech, Aperio, BioImagene Good quality: Hamamatsu, Leica Easy to use: Philips, Claro Flexibility: Olympus dotslide Fast: Philips Cheap: None! Servicio de Salud de Castilla-La Mancha 23/06/
26 Future of slide scanning field Digital pathology adoption trends Slide scanning technology Information (data) management: integration, computer-aided diagnosis Image management: compression, processing and analysis, storage Servicio de Salud de Castilla-La Mancha 23/06/
27 Servicio de Salud de Castilla-La Mancha 23/06/ Digital pathology adoption The biggest barrier to more clinical use is the cost of scanning digital slides, which don t eliminate the need to first prepare glass slides.
28 Servicio de Salud de Castilla-La Mancha 23/06/ Barriers to digital pathology adoption Too expensive... 52% Traditional pathology/microscope works fine... 36% Integration concerns with LIS... 23% Too slow... 15% Reimbursement issues... 13% Limited validated clinical tests... 8% Large data/image storage concerns... 5% No time/patience to learn... 5% Concerns about image resolution... 3% *Survey respondents were able to select multiple answers Source: LE s Digital Pathology Trends Survey, June 2010; n=255
29 Scanning technology If speed and quality needs are reached, then glass slide step can be skipped. Increase automation in Lab workflow Now, many manual steps: Embedding Cutting Staining Permanent Mounting Scanning Reading Store Glass slide In the future, all automated steps: Embedding Cutting Staining Temporal Mounting Scanning Reading Store tissue slice only when needed. No glass slide production Servicio de Salud de Castilla-La Mancha 23/06/
30 Slide scanning technology Polarized light Amiloidosis Synovial effusions Fluorescence scanning FISH Skin and kidney diseases Multiespectral imaging: A series of 3-20 images are taken from blue to the red (e.g nm) Multiplexing: unmixing of three or more chromogens Servicio de Salud de Castilla-La Mancha 23/06/
31 Servicio de Salud de Castilla-La Mancha 23/06/ Future work: Integration with... Pathology information system: Specimen managing, laboratory workflow, and reporting E-Health record: Unique patient database, electronic order of pathology studies, pathology report and images linked with health record, also available from Primary Care
32 Servicio de Salud de Castilla-La Mancha 23/06/ Future work: Integration with... Laboratory devices (stainers, inclusion) PACS: Image Servers Telepathology Portal (telemedicine) Hospital Tumour Registry Biobanks (tumour and tissue banks) Quality Assurance program Image libraries (e.g. education) Clinical guidelines Datawarehouse Data mining
33 Servicio de Salud de Castilla-La Mancha 23/06/ Standards: IHE Integrating Healthcare Enterprise
34 Arquitectura Lógica Servicio de Salud de Castilla-La Mancha 23/06/
35 Servicio de Salud de Castilla-La Mancha 23/06/ Benefits of digital pathology Automating critical processes to optimize and minimize possible sources of error. Diagnosis and therapeutic and prognostic evaluation faster and with more quality, especially relevant in intraoperative studies. Facilitates intra- and interdepartment consultation Better monitoring of the diagnostic process. Introducing the use of processing and image analysis tools to aid in diagnostic pathology.
36 Servicio de Salud de Castilla-La Mancha 23/06/
37 Servicio de Salud de Castilla-La Mancha 23/06/ Muchas gracias.
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