Academic Year CENTRE D ENSEIGNEMENT DE L INSTITUT PASTEUR. Pavillon Louis Martin - Bâtiment N 9. 28, rue du Docteur Roux

Transcription

1 Academic year

2 Academic Year MARCH 3-28, 2014 CENTRE D ENSEIGNEMENT DE L INSTITUT PASTEUR Pavillon Louis Martin - Bâtiment N 9 28, rue du Docteur Roux PARIS Cedex 15 (France) Lectures: Room 2 on the ground floor Practical work: on the ground floor

3 GENERAL PRESENTATION This four-week course is intended for microbiologists (MD, PhD, VetD) practicing mycology in hospital laboratories to catch up with the most recent advances in diagnosis and identification procedures and principles of therapy for fungal infections. Training is provided through bench works, lectures and panel discussions and will cover: Technical aspects : choice of media, slide cultures, storage of isolates, molecular diagnosis and identification (PCR, qpcr, sequencing, MALDI-TOF), typing techniques (microsatellite, MLST), antifungal susceptibility testing (CLSI/EUCAST microbroth dilution methods, commercially available tests), antigen/antibody detection, histopathology. Practical aspects : spectrum of action and pharmacokinetics/dynamics of antifungal drugs, role of the laboratory in the diagnosis and monitoring of patients (direct examination, histopathology, antigen detection, antifungal susceptibility testing results, qpcr ), limitation and use of public and specific databases for molecular identification, discussion of clinical cases. Fungal aspects : sexual and asexual reproduction, phylogeny, species identification, resistance mechanisms. Medical aspects : Pathogenic concepts, epidemiology, diagnosis criteria for infections due to common and emerging yeasts or filamentous fungi (including invasive and mucocutaneous infections, endemic mycoses), principles of therapeutic management, practical guidelines.

4 Medical Mycology Course WEEK 1 - Monday March :00-11:00 Student reception 11:00-13:00 Lecture + bench work: Basic techniques Françoise DROMER Dea GARCIA HERMOSO 14:00-18:00 Bench work: Basic techniques Tuesday March :00-13:00 Lecture + bench work: Sexual reproduction Joëlle DUPONT (Museum National d'histoire Naturelle - Paris) 14:00-18:00 Lecture + bench work: Asexual multiplication Alberto STCHIGEL (Medical School Rovira i Virgili University Reus - Spain) Wednesday March :00-13:00 Bench work: Asexual multiplication (2) Alberto STCHIGEL (Medical School Rovira i Virgili University - Reus - Spain) Dea GARCIA HERMOSO 14:00-18:00 Lecture: Serological tools Stéphane BRETAGNE Olivier LORTHOLARY Thursday March :00-10:00 Lecture: Genome organization of fungi Christophe D ENFERT 10:30-13:00 Lecture: Molecular Biology practical approaches Stéphane BRETAGNE (Institut Pasteur Paris, Hôpital Saint-Louis, Université Paris 7) 14:00-16:00 Lecture: Yeast Identification Procedures Jacques BILLE (Centre Hospitalier Universitaire Vaudois Lausanne - Suisse) 16:00-18:00 Lecture: Mass spectrometry Alexandre ALANIO (Institut Pasteur Paris, Hôpital Saint-Louis, Université Paris 7) Friday March :00-11:00 Lecture: Histology of normal tissue Fabrice CHRETIEN Grégory JOUVION 11:30-13:00 Lecture: Antifungal Drugs/mode of action/pharmacokinetics-dynamics Vincent JULLIEN (Hôpital Européen Georges Pompidou - Paris) 14:00-16:00 Lecture: Yeast Biology Teun BOEKHOUT (CBS KNAW Fungal Biodiversity Centre CT Utrecht - The Netherlands) 16:00-18:00 Bench work: Yeast (1) Françoise DROMER Marie DESNOS-OLLIVIER

5 Medical Mycology course WEEK 2 - Monday March :00-13:00 Bench work: Molecular Biology (yeast and molds) Dea GARCIA HERMOSO 14:00-14:30 Gel deposit Murielle ALMOUSSA 14:30-16:00 Bench work: Yeast (2) Françoise DROMER Marie DESNOS-OLLIVIER 16:00-17:30 Lecture: Polyphasic determination of yeast and molds Dea GARCIA HERMOSO Tuesday March :00-10:45 Lecture + practical discussion: Genotyping methods for fungi Stéphane BRETAGNE (Institut Pasteur Paris, Hôpital Saint-Louis, Université Paris 7) 11:00-13:00 Lecture: Invasive and chronic aspergillosis Johann MAERTENS (University Hospital Gasthuisberg Leuven - Belgium) 14:00-16:30 Lecture: Barcoding and sequence databases Vincent ROBERT (CBS-KNAW Fungal Biodiversity Center - The Netherlands) 16:45-18:15 Bench work 1: Antifungals Françoise DROMER Marie DESNOS-OLLIVIER Wednesday March :00-10:30 Project distribution 10:45-12:45 Lecture: Cryptococcosis Olivier LORTHOLARY (Institut Pasteur - Paris, Hôpital Necker-Enfants maladies, Université Paris 5) 14:00-18:00 Lecture + bench work: Histopathology Fabrice CHRETIEN Grégory JOUVION Thursday March :00-11:00 Lecture: Antifungal susceptibility testing Maiken ARENDRUP (Unit of Mycology Statens Serum Institut Copenhagen Denmark) 11:15-12:45 Lecture: Antifungal drug resistance - mechanism Dominique SANGLARD (Université de Lausanne - Suisse) 13:30-16:00 Lecture: Invasive Candida spp infections Thierry CALANDRA (Centre Hospitalier Universitaire Vaudois (CHUV) Lausanne - Suisse) 16:30-18:00 Time for project Dea GARCIA HERMOSO Friday March :00-11:00 Time for project Dea GARCIA HERMOSO 11:15-13:00 Bench work 2: Antifungal discussion Françoise DROMER Marie DESNOS-OLLIVIER 14:00-16:00 Lecture: Immunity to fungi Anne PUEL (Institut Fédératif de Recherche Necker-Enfants Malades Inserm/Université U Paris) 16:30-18:00 Lecture: Molecular Biology practical approaches / Stéphane BRETAGNE new techniques NGS

6 Medical Mycology course WEEK 3 - Monday March :00-13:00 Lecture + bench work: Keys of Aspergillus identification Paul VERWEIJ Ton RIJS and Hein VAN DER LEE (Radboud University Nijmegen Medical Center Nijmegen - The Netherlands) 14:00-16:30 Bench work: Aspergillus 16:30-18:30 Time for project Tuesday March :00-10:30 Lecture + bench work: Mucorales1 Dea GARCIA HERMOSO 10:30-13:00 Lecture: Cutaneous and mucosal infections (Candida/Malassezia) Rod HAY (International Foundation of Dermatology - London United Kingdom) 14:00-15:30 Lecture: Environmental fungal risk: from the hospital setting to home Jean-Pierre GANGNEUX exposure (CHU Pontchaillou Service de Parasitologie Rennes France) 15:45-18:30 Lecture + bench work: Mucorales2 Dea GARCIA HERMOSO Wednesday March :00-13:00 Lecture + bench work 1: Emerging molds Dea GARCIA HERMOSO 14:00-15:30 Bench work 2: Emerging molds Dea GARCIA HERMOSO 15:30-18:30 Lecture: Emerging molds infections Patricia MUNOZ (Hospital General Universitario Gregorio Marañon Madrid - Spain) Thursday March :00-13:00 Bench work 3+ sequence analysis: Emerging molds Dea GARCIA HERMOSO 14:00-16:00 Lecture: Dimorphic fungi and diagnosis of endemic mycosis Manuel CUENCA ESTRELLA (Centro Nacional de Microbiologia, Instituto de Salud Carlos III Madrid Spain) 16:30-18:30 Time for project Friday March :00-10:30 Time for project 11:00-13:00 Lecture: Mucormycosis and Entomophthoromycosis Fanny LANTERNIER (Hôpital Necker-Enfants malades Université René Descartes Paris-5 Paris) 14:00-16:30 Lecture: Endemic mycoses Olivier LORTHOLARY 16:45-18:30 Time for project

7 Medical Mycology course WEEK 4 - Monday March :00-10:30 Time for project 11:00-13:00 Lecture: Dermatophytes Malcolm RICHARDSON (Regional Mycology Laboratory - Wythenshawe Hospital Manchester - United Kingdom) 14:00-18:00 Bench work: Dermatophytes Tuesday March :00-11:00 Lecture: Animal Mycoses Jacques GUILLOT 11:00-13:00 Time for project Wednesday March (Ecole Nationale Veterinaire d'alfort Maisons Alfort) 9:00-13:00 Revision 14:00-16:00 Written exam Thursday March :00-18:00 Oral exam Friday March :00-12:00 free time 13:00-17:00 Debriefing 17:00-18:30 Brindis

8 EXAM MEDICAL MYCOLOGY COURSE The final mark will include 3 components: 1 Written exam Wednesday March 26th (duration 2 hrs): mark on 1-20 scale, coefficient 1 Accepted language : English, French No document allowed in the classroom 2 Oral exam: (1-20 scale, coefficient 1) Thursday March 27th On March 12 th, a draw will be organized. Each student will get an unknown yeast or mould isolate. Each isolate will be associated with a clinical history. In the following 3 weeks, the student will work to achieve identification to the species level, and will present it on the day of the exam. Each student will prepare a short Powerpoint file to present the entire procedure and the key features allowing identification, including morphology (pictures welcome), differential diagnosis and key elements of the clinical history. On March 26th, there will be another draw to decide the order of the presentations. The powerpoint presentation should be handled to the Head of Studies on March 26th before 2:00 pm. The oral presentation (10 min max) in English (with slides in English) will be performed in front of all students and a committee composed of the Head of Studies and the course directors. Questions will be asked by the committee or the fellow students. 3 Participation to bench work, student s investment, quality of work, participation to discussions will be evaluated throughout the duration of the course by the Head of Studies and the course directors (1-20 scale; coefficient 0.5).