I d like to thank the organizers for this chance to talk to you about our data and for putting together this exciting workshop.

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "I d like to thank the organizers for this chance to talk to you about our data and for putting together this exciting workshop."

Transcription

1 HIV persistence in GALT across different gut sites: role of Tcell recovery, activation and memory subset composition Steven Yukl 1,2, Elizabeth Sinclair 2,3, Lorrie Epling 2,3, Qingsheng Li 4, Amandeep Shergill 1,2, Kenneth McQuaid 1,2, Lijie Duan 4, C. Bradley Hare 2,3, Harry Lampiris 1,2, Ashley Haase 4, Diane Havlir 2,3, Joseph K. Wong 1,2 and PLUS Study Group 1. San Francisco VA Medical Center, CA, USA 2. University of California, San Francisco (UCSF), CA, USA 3. San Francisco General Hospital, CA, USA 4. University of Minnesota, Minneapolis, MN, USA 5. University of Zurich Hospital I d like to thank the organizers for this chance to talk to you about our data and for putting together this exciting workshop. 1

2 Levels of HIV DNA in CD+ T-cells varies across gut sites but exceeds levels found in blood HIV DNA+ cells in GALT based on avg measurements = 1.2x10 9 infected CD4+T-cells Estimated cellular reservoir size in GALT w/ replication competent HIV= 1.2 x 10 7 cells p calculated for Wilcoxon signed rank Yukl et al JID in press, CROI 2010 Satya s talk was a great introduction to the work that I m about to describe. Still, I d like to take a minute to quickly review two studies that our group, lead by Steve Yukl, recently conducted that forms the motivation and basis forthe current work. This is an extension of an observation made by Tae Wook Chun that HIV DNA is highly enriched (up to 10x) in CD4Tcells in GALT. We found that HIV DNA concentrations were indeed higher in all 4gut sites than in blood ranging from 3 fold higher in duodenum to almost 10 fold higher in rectum. If one extrapolates using average values, one arrives at a calculated 1.2 x 10 9 infected cells harbored in the gut at any given time. Assuming conservatively that 1:100 DNA+ cells is not defective, then the RC reservoir in gut of 1.2 x 10 7 cells exceeds some earlier estimates of the reservoir size by 10fold. 2

3 HIV expression from HIV DNA containing cells is paradoxically low in gut compared to blood Median RNA/DNA ratios lower in gut than in PBMC with the exception of terminal ileum. p calculated for Wilcoxon signed rank Yukl et al JID in press, CROI 2010 Trend for large bowel sites to demonstrate lower HIV RNA/DNA ratios than small bowel. Also notable was that despite what would be presumed to be an activating environment, HIV expression here expressed as ratio of HIV RNA/HIV DNA was lower in ¾gut sites than in blood. The terminal ileum was the exception. 3

4 Changes after intensification with raltegravir are compatible with ongoing replication in ileum Yukl et al, CROI 2010 Other special properties of the terminal ileum site were revealed after 12 weeks of treatment intensification with Raltegravir. In the toppanel we see change in HIV RNA expression across sites. On the left, thechange in CD4+ Tcells as a % of Tcells and on the R the change in CD8 Tcells expressing activation markers CD38 and HLA DR. 4

5 Changes after intensification with raltegravir are compatible with ongoing replication in ileum Yukl et al, CROI 2010 The terminal ileum demonstrated a drop in HIV RNA, a rise in CD4% and the greatest reduction in levels of CD8 Tcell activation following treatment intensification. While preliminary and based on a small number of subjects, these data are compatible with ileum as a site of residual viral replication that could be inhibited by the addition of Raltegravir. All gut sites have more infected cells than blood, Infected cells in terminal ileum express more RNA/infected cell, following treatment intensification, a reduction in HIV RNA, a rise in CD4 % and the greatest reduction in Tcell activation is noted in the terminal ileum. 5

6 What factors account for the variation in modes of HIV persistence across sites? 1. Density of CD4 Tcells (cellular recovery) : target cell availibilty or cellular depletion by viral replicaiton or Production. 2. Activation status : Activated peripheral CD4+ Tcells carry more HIV DNA (Chun et al JCI 2005). 3. Memory CD4 Tcell subset composition : Memory T-cell subsets differ in their contribution to HIV persistence in blood. (Chomont et al Nature Med 2009; Avettand-Fenoel CROI 2009). The work now being presented examines three factors that could contribute to the virologic differences between sites in the earlier studies. The first factor is the Tcell density reflecting Tcell recoveryat each site. Here the relationship with ongoing replication could go in either direction with higher CD4 cell densities resulting in more target cells for infection or reflecting reduced Tcell destruction because of better virologiccontrol. Second, assess levels of T activation in part motivated by earlier observations that activated peripheral Tcells carry more HIV DNA. Last, study memory CD4 T cell subset composition, motivated by observations that in peripheral blood memory subsets differ in their levels of HIV DNA. 6

7 Methods Outcome Sample Assay Details 10 HIV+ patients on ART with undetectable plasma VL 8 patients: ileum, rectum (9-10 bx /site) 2 patients: ileum alone (15 bx) 8 age and sex-matched HIV- comparators Biopsies from ileum and/or rectum (4 bx/site) CD4+ T cell content, T cell activation markers Memory T cell subsets 1. Gut biopsy 2. Gut cells, PBMC Gut cells, PBMC Immunohistochemistry Flow cytometry Flow cytometry CD3, CD4 CD45, CD3, CD4, CD8, CD38, HLA-DR CD45, CD3, CD4, CD8, CD45 Ro/Ra, CCR7, CD27 Cell-associated HIV DNA Gut cells, PBMC Real time PCR Locked Nucleic Acid probe Detection Limit: 1-10 copies The patients studied included 10 HIV + and 8 HIV neg subjects. The work described today focused on the ileum and rectum. Intact gut biopsy samples were studied by immunohistochemistry by Qing Shing li and Ashley Haase but these data will only be mentioned. Isolated gut cells and PBMC were studied by flow cytometry usingconventional Tcell markers and markers of activation. In a subset of three subjects, extended panels with ab recognizing CD45 Ro, CCR7 and CD27 were used to study and sort memory subsets. We used a sensitive in house Taqman PCR for total HIV DNA quantitation. 7

8 CD4+ T cell numbers remain low in the ileum but may be normal in the rectum By Immunohistochemistry, CD4 numbers in ileum are lower for HIV+ on ART in lamina propria but are nl in lymphoid aggregates Measured as a % of all cells (upper panel) or only of Tcells (Lower panel), CD4+ Tcell percentages in treated HIV + patients were lower than in sero-neg controls for PBMC and ileum but not for rectum. Data from HIV+ subjects are in gold and HIV controls in white for PBMC, ileum and rectum. The defect in Tcell recovery in ileum seems, based on observations by IHC by Qing Shing Li and Ashley Haase, to be primarily the lamina propria lymphocytes. This incomplete normalization of CD4 cell % in ileum is consistent with the higher RNA/DNA ratios in ileum and the evidence for ongoing replicationduring treatment intensification we noted earlier. 8

9 T cell activation remains high in blood and both gut sites Levels of activation marker + cells higher in gut sites than in PBMC No differences seen in levels of activation markers between gut sites. In contrast to regional differences in CD4 cell recovery, markers for Tcell activation were higher from treated HIV+ subjects at all sites (shown again in gold bars) when compared with seronegative controls (white bars). Overall levels of activation markers were generally higher in gut sites than in PBMC for both sero positive and sero negative subjects as one might expect. However there were no significant differences in levels of activation measured by these two markers do not seem to explain the different behavior of residual virus at these two sites. 9

10 CD4+ T Cell Memory Subtypes Vary by HIV Status and Site (n=3) CD45 RO CCR7 CD27 Naive - + Terminally differentiated - - Central Memory Effector Memory Transitional Memory Other Memory Next we compared the Tcell memory subset composition from the blood and the two sites. (Here I think the panels are reversed and the lower panel reflects data from HIV sero negative subjects). So as expected, in the HIV+ subjects we see a slight contractionof naïve and effector memory cell populations and maybe an expansion of transitional memory subsets. 10

11 CD4+ T Cell Memory Subtypes Vary by HIV Status and Site Greater proportion of TM and EM cells could contribute to the greater concentration of HIV DNA+ cells in rectal mucosa. Qualitatively the subset distribution in rectum was not very different between HIV + and HIV subjects but the between site differences between blood and rectum with enrichment of TM and EM populations (at the expense of naïve and CM populations ) could explain part of the enrichment for HIV DNA+ cells for some patients. However we estimate that this should account for a 2 and possibly three fold enrichment at most not the 9 fold differences seen. 11

12 CD4+ T Cell Memory Subtypes Vary by HIV Status and Site The subset distribution is similar between rectum and ileum fromhiv negative subjects but a large population of CD45Ro+ CCR7+ CD27 other memory cells is seen in ileal tissue of HIV+ subjects but not of controls and not in rectal tissues. Whether these represent a dynamic population in transition or are a stable population is not known. Such other memory phenotypes are seen after ex vivo Tcell activation. It will be interesting to see to what degree these cells are infected or are a target for viral replication. As with the rectum, these differences in subtype distribution themselves probably do not explain all of the inter-site differences in HIV DNA content. 12

13 HIV DNA (per 10 6 cells) by Cell Type in Sorted Cells from Blood and Ileum HIV DNA in: Blood Terminal Ileum Transitional Memory (TM) CD4+ T Cells Effector Memory (EM) CD4+ T Cells 175 3, Remaining CD4+ T Cells 344 ND (<482) Leukocytes other than CD4+ T cells ND (<4) ND (<17) ND=not detected To determine whether HIV DNA content was the same in a single memory subset we sorted TM and EM and all other CD4 Tcells (as well as non-cd4 expressing leukocytes) and measured HIV DNA. Values are mean from two patients of HIVDNA copies/million cells In terminal ileum, cells with the TM phenotype contained higher levels of HIV DNA than in blood but no differences were seen for EM cells. For HIV DNA not detected samples, we include a upper bound for potential copy numbers based on available sample for testing. With the caveat that this is only based on two patients, these data suggest that cells of the same memory subset phenotype can contain vastly different HIV DNA levels. It remains to be seen whether this is the case at other GALT sites and to be determined whether such infected cells are being preferentially infected denovo in GALT or expanded as a consequence of preferential proliferation of infected cells. 13

14 Summary/Conclusions CD4 numbers remain abnormally low in the ileum but approach normal in the rectum. Tcell activation remains high in blood and at both gut sites. Abnormal T cell activation and incomplete CD4 reconstitution in the ileum are compatible with a site that favors ongoing virus production or replication. CD4 numbers remain low in ileum but appear normal in rectum. Compared with seronegative controls, Tcell activation is high atall sites and not different between sites. Abnormal Tcell activation and incomplete CD4 Tcell reconsitutionin the ileum are compatible with a site favorable to either ongoing replication or chronic viral production. 14

15 Summary/Conclusions Memory subset distributions differ between blood and gut and among gut sites. Ileum may have an abnormal distribution of memory Tcell subtypes ( TM and EF, CD45Ro+/CCR7+/CD27-). Within a single memory subset, the frequency of infected cells may differ between sites. Differences in Tcell memory subset composition as well as different rates of infection account for the higher HIV DNA content in CD4 Tcells in gut. Memory subsets in gut differ across sites and compared to blood. Ileum appears to have an abnormal distribution of memory Tcell subtypes. Within a single memory subset, HIV infection rates can differ between sites. Both differences in Tcell memory composition and the rates of infection for each subset account for the higher HIV DNA content seen in GALT. Future work to define the roles that memory subsets play in propagating infection or amplifying virus by homeostatic proliferation and cell division should also target the GALT. 15

16 Acknowledgement UCSF SF VAMC Steven Yukl Peilin Li Katsuya Fujimoto Alex Choi SF VAMC GI & ID Amandeep Shergill Ken McQuaid Harry Lampiris Paul Volberding Participants UCSF SF Gen Hosp Diane Havlir Brad Hare Michelle Downing Marc Gould Hiroyu Hatano Steven Deeks U of Minnesota Qing Li Ashley Haase Funding support Dept of VA NIH UCSF CFAR University of Zürich Marek Fischer Sara Gianella Huldrych Günthard UCSF CFAR Imm Core Elizabeth Sinclair Lorrie Eppling Terrence Ho

17 Background: HIV infected cells persist despite HAART Anatomic reservoirs Peripheral blood CNS Genital tract tissues Central lymphoid tissues Mucosal lymphoid tissues Phenotypically discrete cellular reservoirs Central memory CD+ Tcells Monocyte/macrophages Microglial cells/astroglial cells Other memory Tcell subsets

PATHOGENESIS OF AIDS

PATHOGENESIS OF AIDS Neal Nathanson, MD Emeritus Professor Department of Microbiology University of Pennsylvania School of Medicine 1 Definitions Structure of HIV HIV entry and cell tropism HIV-cell interactions Course of

More information

Measuring the HIV Reservoir BINGO Review Activity

Measuring the HIV Reservoir BINGO Review Activity Measuring the HIV Reservoir BINGO Review Activity Objectives Describe the differences in current technologies available for measuring the HIV reservoir Discuss the risk and benefit of each technology Methods

More information

Longitudinal Analysis of Lymphocyte Ratios and HIV-1 Intracellular DNA Levels in Children

Longitudinal Analysis of Lymphocyte Ratios and HIV-1 Intracellular DNA Levels in Children BRIEF REPORT Longitudinal Analysis of Lymphocyte Ratios and HIV-1 Intracellular DNA Levels in Children Akihiko Saitoh, 1 Christine A. Powell, 4 Terence Fenton, 4 Steven D. Douglas, 5 Stuart E. Starr, 5

More information

Réservoirs VIH : du contrôle à l'éradication. Asier Sáez-Cirión, PhD Unité de Régulation des Infections Rétrovirales

Réservoirs VIH : du contrôle à l'éradication. Asier Sáez-Cirión, PhD Unité de Régulation des Infections Rétrovirales Réservoirs VIH : du contrôle à l'éradication Asier Sáez-Cirión, PhD Unité de Régulation des Infections Rétrovirales M. Coiras et al Nat Rev Micro 2009 HIV-1 life cycle and latency HIV-1 life cycle and

More information

HIV Diagnosis. HIV Diagnosis and Pathogenesis. Laboratory Diagnosis of Established HIV Infection: Antibody Detection

HIV Diagnosis. HIV Diagnosis and Pathogenesis. Laboratory Diagnosis of Established HIV Infection: Antibody Detection HIV Diagnosis HIV Diagnosis and Pathogenesis Scott M. Hammer, M.D. Consider in anyone presenting with symptoms and signs compatible with an HIV-related syndrome or in an asymptomatic person with a risk

More information

New Features of PML in the HAART and natalizumab era

New Features of PML in the HAART and natalizumab era New Features of PML in the HAART and natalizumab era Igor J. Koralnik, M.D. HIV/Neurology Center Beth Israel Deaconess Medical Center Harvard Medical School Classical AIDS-associated PML in the pre-haart

More information

The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time

The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time Lina Josefsson a,b,1, Susanne von Stockenstrom a,b, Nuno R. Faria c, Elizabeth

More information

Reconsideration Code 86386. Reconsideration Code Description Nuclear Matrix Protein 22 (NMP22), qualitative

Reconsideration Code 86386. Reconsideration Code Description Nuclear Matrix Protein 22 (NMP22), qualitative Calendar Year 2013 Centers for Medicare and Medicaid Services (CMS) New and Reconsidered Clinical Laboratory Fee Schedule (CLFS) Test Codes And Final Payment Determinations Reconsideration Code 86386 Reconsideration

More information

Acute HIV infection: Implication for HIV Control and Cure

Acute HIV infection: Implication for HIV Control and Cure Acute HIV infection: Implication for HIV Control and Cure Jintanat Ananworanich, MD, PhD Deputy Director in Scientific Affairs, HIV-NAT Chief, SEARCH The Thai Red Cross AIDS Research Centre Bangkok, Thailand

More information

Bromodeoxyuridine (BrdU): Current Status

Bromodeoxyuridine (BrdU): Current Status Studies of Lymphocyte Kinetics in HIV Infection To understand the effects on lymphocyte turnover of: HIV infection HAART therapy Interleukin-2 therapy Methods-in vivo labeling BrdU Deuterated glucose Bromodeoxyuridine

More information

2011 Update on the ECIL-3 guidelines for EBV management in patients with leukemia and other hematological disorders

2011 Update on the ECIL-3 guidelines for EBV management in patients with leukemia and other hematological disorders UPDATE ECIL-4 2011 2011 Update on the ECIL-3 guidelines for EBV management in patients with leukemia and other hematological disorders Jan Styczynski, Hermann Einsele, Rafael de la Camara, Catherine Cordonnier,

More information

Multiple Myeloma and Colorectal Cancer

Multiple Myeloma and Colorectal Cancer Multiple Myeloma and Colorectal Cancer From Systems Immunology to Single Cells Leo Hansmann Mark M. Davis Lab Department of Microbiology&Immunology Stanford University Multiple Myeloma Monoclonal disease

More information

HIV Drug resistanceimplications

HIV Drug resistanceimplications HIV Drug resistanceimplications for therapy Deenan Pillay Africa Centre for Health and Population Studies, UKZN University College London Potential implications of HAART without virological monitoring:

More information

This presentation on low grade B-cell lymphomas is meant to provide a general overview of the following:

This presentation on low grade B-cell lymphomas is meant to provide a general overview of the following: This presentation on low grade B-cell lymphomas is meant to provide a general overview of the following: 1. Grading lymphomas: why do we grade lymphomas, and why is it important. 2. Categories: what are

More information

Respondent s Exhibit QQ

Respondent s Exhibit QQ Respondent s Exhibit QQ IN THE UNITED STATES COURT OF FEDERAL CLAIMS OFFICE OF SPECIAL MASTERS THERESA CEDILLO and MICHAEL CEDILLO, as Parents and Natural Guardians of MICHELLE CEDILLO, Petitioners, No.

More information

Allogeneic stem cell transplant in HIV-1-infected individuals

Allogeneic stem cell transplant in HIV-1-infected individuals Allogeneic stem cell transplant in HIV-1-infected individuals Javier Martinez-Picado UNIVERSITAT DE VIC Barriers to cure HIV infection Residual Replication Immune activation Inflammation Latent Infection

More information

Aktuell HIV-forskning 2014-05-06

Aktuell HIV-forskning 2014-05-06 Aktuell HIV-forskning 2014-05-06 Alexey Kashpersky Aktuell HIV-forskning Bot Smittsamhet Nya läkemedel HIV och åldrande Etc HPTN 052 Prevention Conclusion Early ART that suppresses viral replication led

More information

REAL-TIME PCR KITS FOR DIAGNOSIS

REAL-TIME PCR KITS FOR DIAGNOSIS REAL-TIME PCR KITS FOR DIAGNOSIS 483 Reproducible, Sensitive and Precise Analysis Real Time PCR (qpcr) has revolutionized biological and medical sciences by providing quantitative detection of specific

More information

Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159. Date Submitted: 12-11-09

Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159. Date Submitted: 12-11-09 Title: Mapping T cell epitopes in PCV2 capsid protein - NPB #08-159 Investigator: Institution: Carol Wyatt Kansas State University Date Submitted: 12-11-09 Industry summary: Effective circovirus vaccines

More information

Diagnostic Testing and Strategies for BVDV

Diagnostic Testing and Strategies for BVDV Diagnostic Testing and Strategies for BVDV Dan Grooms Dept. of Large Animal Clinical Sciences College of Veterinary Medicine Introduction Clinical diseases in cattle resulting from infection with bovine

More information

bitter is de pil Linos Vandekerckhove, MD, PhD

bitter is de pil Linos Vandekerckhove, MD, PhD 4//24 Current HIV care HIV copies/ ml plasma Viral load Welcome to the Digital droplet PCR age! bitter is de pil Linos Vandekerckhove, MD, PhD Latent HIV reservoir Time at Ghent University Hospital 2 HIV

More information

Deep profiling of multitube flow cytometry data Supplemental information

Deep profiling of multitube flow cytometry data Supplemental information Deep profiling of multitube flow cytometry data Supplemental information Kieran O Neill et al December 19, 2014 1 Table S1: Markers in simulated multitube data. The data was split into three tubes, each

More information

Early HIV- specific CD8 + T cell responses in treated and untreated hyper acute HIV infec=on in the FRESH cohort

Early HIV- specific CD8 + T cell responses in treated and untreated hyper acute HIV infec=on in the FRESH cohort Early HIV- specific CD8 + T cell responses in treated and untreated hyper acute HIV infec=on in the FRESH cohort Zaza M&ne Ndhlovu, PhD Instructor in Medicine Ragon Ins&tute of MGH, MIT and Harvard Senior

More information

La sospensione dell ARV è sempre una cattiva idea?

La sospensione dell ARV è sempre una cattiva idea? 15/05/2014 La sospensione dell ARV è sempre una cattiva idea? Adriano Lazzarin IRCCS Ospedale San Raffaele, Università Vita e Salute, Milano 6 Infectivology Today I due principi che guideranno il management

More information

Supplemental Information. McBrayer et al. Supplemental Data

Supplemental Information. McBrayer et al. Supplemental Data 1 Supplemental Information McBrayer et al. Supplemental Data 2 Figure S1. Glucose consumption rates of MM cell lines exceed that of normal PBMC. (A) Normal PBMC isolated from three healthy donors were

More information

NS5B Sequencing and Phenotypic Resistance Assays for HCV Subtypes 1a and 1b

NS5B Sequencing and Phenotypic Resistance Assays for HCV Subtypes 1a and 1b NS5B Sequencing and Phenotypic Resistance Assays for HCV Subtypes 1a and 1b 5th Intl. Workshop on Hepatitis C Resistance & New Compounds Jacqueline Reeves NS5B Resistance Assays for HCV Subtypes 1a and

More information

The immune system. Bone marrow. Thymus. Spleen. Bone marrow. NK cell. B-cell. T-cell. Basophil Neutrophil. Eosinophil. Myeloid progenitor

The immune system. Bone marrow. Thymus. Spleen. Bone marrow. NK cell. B-cell. T-cell. Basophil Neutrophil. Eosinophil. Myeloid progenitor The immune system Basophil Neutrophil Bone marrow Eosinophil Myeloid progenitor Dendritic cell Pluripotent Stem cell Lymphoid progenitor Platelets Bone marrow Thymus NK cell T-cell B-cell Spleen Cancer

More information

QUICK CELL CAPTURE AND CHARACTERIZATION GUIDE FOR CELLSEARCH CUSTOMERS

QUICK CELL CAPTURE AND CHARACTERIZATION GUIDE FOR CELLSEARCH CUSTOMERS QUICK CELL CAPTURE AND CHARACTERIZATION GUIDE FOR CELLSEARCH CUSTOMERS CellSave EDTA Blood sample Rare cell capture Enumeration Single protein marker Cell capture for molecular characterization CELLSEARCH

More information

D Candotti. Institut National de la Transfusion Sanguine Dept. Agents Transmissibles par le Sang Paris, France

D Candotti. Institut National de la Transfusion Sanguine Dept. Agents Transmissibles par le Sang Paris, France Molecular characterization of hepatitis B virus strains infecting blood donors with high HBsAg and undetectable HBV DNA levels: implications for blood safety and screening policy D Candotti Institut National

More information

The Costimulatory Molecule CD27 Maintains Clonally

The Costimulatory Molecule CD27 Maintains Clonally Immunity, Volume 35 Supplemental Information The Costimulatory Molecule CD7 Maintains Clonally Diverse CD8 + T Cell Responses of Low Antigen Affinity to Protect against Viral Variants Klaas P.J.M. van

More information

INTERPRETATION INFORMATION SHEET

INTERPRETATION INFORMATION SHEET Creative Testing Solutions 2424 West Erie Dr. 2205 Highway 121 10100 Martin Luther King Jr. St. No. Tempe, AZ 85282 Bedford, TX 76021 St. Petersburg, FL 33716 INTERPRETATION INFORMATION SHEET Human Immunodeficiency

More information

Rouch, Jean. Cine-Ethnography. Minneapolis, MN, USA: University of Minnesota Press, 2003. p 238

Rouch, Jean. Cine-Ethnography. Minneapolis, MN, USA: University of Minnesota Press, 2003. p 238 Minneapolis, MN, USA: University of Minnesota Press, 2003. p 238 http://site.ebrary.com/lib/uchicago/doc?id=10151154&ppg=238 Minneapolis, MN, USA: University of Minnesota Press, 2003. p 239 http://site.ebrary.com/lib/uchicago/doc?id=10151154&ppg=239

More information

The Tale of two Herpes Viruses: CMV and EBV

The Tale of two Herpes Viruses: CMV and EBV Immunocompromised PIDPIC Pediatric Infectious Diseases The Tale of two Herpes Viruses: CMV and EBV Sharon F. Chen, M.D., M.S. Hayley Gans, M.D. Pediatric Infectious Diseases Pediatric Infectious Diseases

More information

Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle

Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle Productive HIV-1 infection of human cervical tissue ex vivo is associated with the secretory phase of the menstrual cycle Saba E. et al. Introduction HIV-1 deposited in mucosa of lower female reproductive

More information

Cytoreductive Therapy for Autologous Cell Therapy in HIV

Cytoreductive Therapy for Autologous Cell Therapy in HIV Cytoreductive Therapy for Autologous Cell Therapy in HIV Ronald Mitsuyasu, MD Professor of Medicine UCLA Center for Clinical AIDS Research and Education (CARE Center) HSC Transfer from CCR5-delta 32 Donor

More information

Viral load testing. medical monitoring: viral load testing: 1

Viral load testing. medical monitoring: viral load testing: 1 medical monitoring: viral load testing: 1 medical monitoring: viral load testing Viral load testing medical monitoring: viral load testing: 2 Slide 1 Viral load The viral load test measures HIV in the

More information

SYMPOSIUM. June 15, 2013. Photonics Center Colloquium Room (906) 8 Saint Mary's St., Boston, MA 02215 Boston University

SYMPOSIUM. June 15, 2013. Photonics Center Colloquium Room (906) 8 Saint Mary's St., Boston, MA 02215 Boston University SYMPOSIUM June 15, 2013 Photonics Center Colloquium Room (906) 8 Saint Mary's St., Boston, MA 02215 Bette Korber Los Alamos National Labs HIV evolution and the neutralizing antibody response We have recently

More information

Uses of Flow Cytometry

Uses of Flow Cytometry Uses of Flow Cytometry 1. Multicolour analysis... 2 2. Cell Cycle and Proliferation... 3 a. Analysis of Cellular DNA Content... 4 b. Cell Proliferation Assays... 5 3. Immunology... 6 4. Apoptosis... 7

More information

HIV Testing. Conventional laboratory tests and some new developments Molecular Diagnosis

HIV Testing. Conventional laboratory tests and some new developments Molecular Diagnosis HIV Testing Conventional laboratory tests and some new developments Molecular Diagnosis NSW State Reference Laboratory for HIV Centre for Applied Medical Research St Vincent s Hospital Sydney This presentation

More information

The Humoral Immune system Structure and Diversity

The Humoral Immune system Structure and Diversity The Humoral Immune system Structure and Diversity Discussion: Introduction Our immune system protects our bodies from the harmful affects of a dizzying array of disease causing pathogens. Although our

More information

About Our Products. Blood Products. Purified Infectious/Inactivated Agents. Native & Recombinant Viral Proteins. DNA Controls and Primers for PCR

About Our Products. Blood Products. Purified Infectious/Inactivated Agents. Native & Recombinant Viral Proteins. DNA Controls and Primers for PCR About Our Products Purified Infectious/Inactivated Agents ABI produces a variety of specialized reagents, allowing researchers to choose the best preparations for their studies. Available reagents include

More information

On the origin of giant multinuclear Reed-Sternberg cells and the role of CD4 T cells in Hodgkin lymphoma

On the origin of giant multinuclear Reed-Sternberg cells and the role of CD4 T cells in Hodgkin lymphoma On the origin of giant multinuclear Reed-Sternberg cells and the role of CD4 T cells in Hodgkin lymphoma Uber die Entstehung von multinuklearen Reed-Sternberg Riesenzellen und die Rolle von CD4 T-Zellen

More information

Identification of T-cell epitopes of SARS-coronavirus for development of peptide-based vaccines and cellular immunity assessment methods

Identification of T-cell epitopes of SARS-coronavirus for development of peptide-based vaccines and cellular immunity assessment methods RESEARCH FUND FOR THE CONTROL OF INFECTIOUS DISEASES PKS Chan 陳 基 湘 S Ma 文 子 光 SM Ngai 倪 世 明 Key Messages 1. Subjects recovered from SARS-CoV infection retain memory of cellular immune response to epitopes

More information

Need for Expansion of BTS. Insufficient Space

Need for Expansion of BTS. Insufficient Space For discussion on 17 November 2014 LC Paper No. CB(2)242/14-15(07) Legislative Council Panel on Health Services Expansion of the Hong Kong Red Cross Blood Transfusion Service Headquarters Purpose This

More information

Chromosomal analysis for prenatal samples, peripheral blood, bone marrow biopsy samples, various types of lymphomas, and solid tumors

Chromosomal analysis for prenatal samples, peripheral blood, bone marrow biopsy samples, various types of lymphomas, and solid tumors Chromosomal analysis for prenatal samples, peripheral blood, bone marrow biopsy samples, various types of lymphomas, and solid tumors FISH (fluorescence in situ hybridization) assays and many probes that

More information

Viral Hepatitis Case Report

Viral Hepatitis Case Report Page 1 of 9 Viral Hepatitis Case Report Perinatal Hepatitis B Virus Infection Michigan Department of Community Health Communicable Disease Division Investigation Information Investigation ID Onset Date

More information

Hepatitis C Monitoring and Complications (and Treatment!) Dr Mark Douglas

Hepatitis C Monitoring and Complications (and Treatment!) Dr Mark Douglas Hepatitis C Monitoring and Complications (and Treatment!) Dr Mark Douglas Hepatitis C Virus Shimizu et al., 1996 Positive single strand RNA virus Flaviviridae family, Hepacivirus genus 9.6 kbp genome ~3000

More information

How do you know if you almost cured someone of HIV?

How do you know if you almost cured someone of HIV? How do you know if you almost cured someone of HIV? PR 691: Identification of Biomarkers to Predict Time to Plasma HIV RNA Rebound and Post-Treatment Viral Control during an Intensely Monitored Antiretroviral

More information

Pitfalls of PCR diagnosis of viral infections

Pitfalls of PCR diagnosis of viral infections Pitfalls of PCR diagnosis of viral infections David Whiley Queensland Paediatric Infectious Diseases Laboratory, QCMRI & SASVRC, Royal Children's Hospital, Brisbane. PCR & viral infections PCR technology

More information

Human Peripheral Blood Mononuclear Cell (PBMC) Manual

Human Peripheral Blood Mononuclear Cell (PBMC) Manual Human Peripheral Blood Mononuclear Cell (PBMC) Manual INSTRUCTION MANUAL ZBM0063.04 SHIPPING CONDITIONS Human Peripheral Blood Mononuclear Cells, cryopreserved Cryopreserved human peripheral blood mononuclear

More information

Specialty Lab and Immunological Testing Services

Specialty Lab and Immunological Testing Services Specialty Lab and Immunological Testing Services OVER 30 YEARS OF SPECIALTY LAB EXPERIENCE Precision for Medicine recognizes the critical role that sample testing and handling play in achieving high-quality,

More information

Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives

More information

Overview of HIV Testing

Overview of HIV Testing Overview of HIV Testing Mark Gilbert, MD Director, Applied Epidemiology Unit Ontario HIV Treatment Network October 26 2015 Harm Reduction Conference Objectives To answer the following questions: 1. What

More information

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10

VLLM0421c Medical Microbiology I, practical sessions. Protocol to topic J10 Topic J10+11: Molecular-biological methods + Clinical virology I (hepatitis A, B & C, HIV) To study: PCR, ELISA, your own notes from serology reactions Task J10/1: DNA isolation of the etiological agent

More information

HPV DNA Testing: Clinical vs. Analytical Sensitivity

HPV DNA Testing: Clinical vs. Analytical Sensitivity HPV DNA Testing: Clinical vs. Analytical Sensitivity The digene HPV Test Sample & Assay Technologies How to validate an HPV test for optimal clinical performance Clinical sensitivity more accurately represents

More information

T Cell Maturation,Activation and Differentiation

T Cell Maturation,Activation and Differentiation T Cell Maturation,Activation and Differentiation Positive Selection- In thymus, permits survival of only those T cells whose TCRs recognize self- MHC molecules (self-mhc restriction) Negative Selection-

More information

SWOG ONCOLOGY RESEARCH PROFESSIONAL (ORP) MANUAL VOLUME I RESPONSE ASSESSMENT LEUKEMIA CHAPTER 11A REVISED: OCTOBER 2015

SWOG ONCOLOGY RESEARCH PROFESSIONAL (ORP) MANUAL VOLUME I RESPONSE ASSESSMENT LEUKEMIA CHAPTER 11A REVISED: OCTOBER 2015 LEUKEMIA Response in Acute Myeloid Leukemia (AML) Response criteria in Acute Myeloid Leukemia for SWOG protocols is based on the review article Diagnosis and management of acute myeloid leukemia in adults:

More information

IKDT Laboratory. IKDT as Service Lab (CRO) for Molecular Diagnostics

IKDT Laboratory. IKDT as Service Lab (CRO) for Molecular Diagnostics Page 1 IKDT Laboratory IKDT as Service Lab (CRO) for Molecular Diagnostics IKDT lab offer is complete diagnostic service to all external customers. We could perform as well single procedures or complex

More information

Oncos Therapeutics: ONCOS THERAPEUTICS Personalized Cancer Immunotherapy. March 2015. Antti Vuolanto, COO and co-founder

Oncos Therapeutics: ONCOS THERAPEUTICS Personalized Cancer Immunotherapy. March 2015. Antti Vuolanto, COO and co-founder Oncos Therapeutics: Personalized Cancer Immunotherapy ONCOS THERAPEUTICS Personalized Cancer Immunotherapy March 2015 Antti Vuolanto, COO and co-founder 1 History of Oncos Therapeutics 2002 2007 2009 Research

More information

Dr Paul Turner. Research Scientist Dunedin

Dr Paul Turner. Research Scientist Dunedin Dr Paul Turner Research Scientist Dunedin Spinal Cord Society NZ Inc. Who: Aim: Started by Noela & Keith Vallis & people with spinal cord injury (SCI), their families and care-givers in 1988. To support

More information

Master Answers for the Autoimmune Disease Small group

Master Answers for the Autoimmune Disease Small group Master Answers for the Autoimmune Disease Small group Master Answer for Cases 1A and 1B There are multiple possible etiologies of autoimmune endocrinopathies, but regardless of the precise cause, there

More information

LYMPHOMA. BACHIR ALOBEID, M.D. HEMATOPATHOLOGY DIVISION PATHOLOGY DEPARTMENT Columbia University/ College of Physicians & Surgeons

LYMPHOMA. BACHIR ALOBEID, M.D. HEMATOPATHOLOGY DIVISION PATHOLOGY DEPARTMENT Columbia University/ College of Physicians & Surgeons LYMPHOMA BACHIR ALOBEID, M.D. HEMATOPATHOLOGY DIVISION PATHOLOGY DEPARTMENT Columbia University/ College of Physicians & Surgeons Normal development of lymphocytes Lymphocyte proliferation and differentiation:

More information

Laboratory diagnosis of viral infections. Specimen collection and transport

Laboratory diagnosis of viral infections. Specimen collection and transport Laboratory diagnosis of viral infections Specimen collection and transport Steps in Pathogenesis of Viral Diseases 1. Entry through skin through mucosa (respiratory, gastrointestinal or genital) through

More information

RealLine HCV PCR Qualitative - Uni-Format

RealLine HCV PCR Qualitative - Uni-Format Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013

More information

Coding and Billing for HIV Services in Healthcare Facilities

Coding and Billing for HIV Services in Healthcare Facilities P a g e 1 Coding and Billing for HIV Services in Healthcare Facilities The Hawai i State Department of Health STD/AIDS Prevention Branch is pleased to provide you information on billing and reimbursement

More information

Lymphocyte Recirculation

Lymphocyte Recirculation Chapter 5 Lymphocyte Migration and Inflammation Lymphocyte Recirculation Naïve lymphocytes enter lymph nodes from the blood circulation Lymphocytes return to blood via the thoracic duct Antigens from infected

More information

Human Genome Complexity, Viruses & Genetic Variability

Human Genome Complexity, Viruses & Genetic Variability Human Genome Complexity, Viruses & Genetic Variability (Learning Objectives) Learn the types of DNA sequences present in the Human Genome other than genes coding for functional proteins. Review what you

More information

The Adaptive Immune Response. B-cells

The Adaptive Immune Response. B-cells The Adaptive Immune Response B-cells FUNCTIONS OF THE IMMUNE SYSTEM: Recognize, destroy and clear a diversity of pathogens. Initiate tissue and wound healing processes. Recognize and clear damaged self

More information

Identification of Tick-Borne Encephalitis Virus CD4+ and CD8+ T-Cell Epitopes in Vaccinated or Naturally Infected Humans

Identification of Tick-Borne Encephalitis Virus CD4+ and CD8+ T-Cell Epitopes in Vaccinated or Naturally Infected Humans Identification of Tick-Borne Encephalitis Virus CD4+ and CD8+ T-Cell Epitopes in Vaccinated or Naturally Infected Humans Schwaiger Julia Clinical Institute of Virology 29.06.2009 1 Thank you! Franz X.

More information

HIV and Hepatitis B CoInfection

HIV and Hepatitis B CoInfection HIV and Hepatitis B CoInfection Douglas G. Fish, MD June 3, 2014 44 yo male with AIDS who had fallen out of care and returned in October 2013 Last seen in November 2012 CD4 at that time 340 cells/cmm HIV

More information

Viral Safety of Plasma-Derived Products

Viral Safety of Plasma-Derived Products Viral Safety of Plasma-Derived Products SLIDE 1 This presentation will cover viral validation studies for plasma-derived products. FDA requires that the manufacturing process for biopharmaceutical products

More information

Lab 1: Who s Your Daddy? (AKA DNA Purification and PCR)

Lab 1: Who s Your Daddy? (AKA DNA Purification and PCR) Lab 1: Who s Your Daddy? (AKA DNA Purification and PCR) Goals of the lab: 1. To understand how DNA s chemical properties can be exploited for purification 2. To learn practical applications of DNA purification

More information

Clinical significance of routine immunological markers in patients infected with influenza type A H1N1 virus

Clinical significance of routine immunological markers in patients infected with influenza type A H1N1 virus 198 2009,,,, ( ), 201508 : 2009 ( ) ( H1N1) 32 H1N1 T,,, H1N1 CD3 + CD3 + CD4 + CD3 + CD8 + T ( cells/ l), C3 C4( g/l) C ( mg/l),,, H1N1 : H1N1; T ; Clinical significance of routine immunological markers

More information

1 2 3 4 5 6 Figure 4.1: Gel picture showing Generation of HIV-1subtype C codon optimized env expressing recombinant plasmid pvax-1:

1 2 3 4 5 6 Figure 4.1: Gel picture showing Generation of HIV-1subtype C codon optimized env expressing recombinant plasmid pvax-1: Full-fledged work is in progress towards construction and cloning of codon optimized envelope with subsequent aims towards immunization of mice to study immune responses. 1 2 4 5 6 Figure 4.1: Gel picture

More information

WHICH SAMPLES SHOULD BE SUBMITTED WHEN LYMPHOID NEOPLASIA IS SUSPECTED?

WHICH SAMPLES SHOULD BE SUBMITTED WHEN LYMPHOID NEOPLASIA IS SUSPECTED? WHICH SAMPLES SHOULD BE SUBMITTED WHEN LYMPHOID NEOPLASIA IS SUSPECTED? Which test should be submitted? The answer to this depends on the clinical signs, and the diagnostic question you are asking. If

More information

Mid-Clinical Stage Antiviral Drug Development Company

Mid-Clinical Stage Antiviral Drug Development Company BIOTRON LIMITED (ASX:BIT) Mid-Clinical Stage Antiviral Drug Development Company Investor Update 20 August 2015 Dr Michelle Miller Managing Director +61 412 313329 mmiller@biotron.com.au www.biotron.com.au

More information

Chapter 6. Antigen-Antibody Properties 10/3/2012. Antigen-Antibody Interactions: Principles and Applications. Precipitin reactions

Chapter 6. Antigen-Antibody Properties 10/3/2012. Antigen-Antibody Interactions: Principles and Applications. Precipitin reactions Chapter 6 Antigen-Antibody Interactions: Principles and Applications Antigen-Antibody Properties You must remember antibody affinity (single) VS avidity (multiple) High affinity: bound tightly and longer!

More information

Immune Changes in Tumor- Draining Lymph Nodes as Novel Biomarkers. Peter P. Lee, M.D. Dept. of Medicine (Hem) Stanford University

Immune Changes in Tumor- Draining Lymph Nodes as Novel Biomarkers. Peter P. Lee, M.D. Dept. of Medicine (Hem) Stanford University Immune Changes in Tumor- Draining Lymph Nodes as Novel Biomarkers Peter P. Lee, M.D. Dept. of Medicine (Hem) Stanford University TDLNs in Breast Cancer Tumor invasion of TDLNs is a key determinant of clinical

More information

Biopharmaceutical Process Evaluated for Viral Clearance

Biopharmaceutical Process Evaluated for Viral Clearance Authored by S. Steve Zhou, Ph.D. Microbac Laboratories, Inc., Microbiotest Division The purpose of Viral Clearance evaluation is to assess the capability of a manufacturing production process to inactivate

More information

Flow cytometric approach to the diagnosis and classification of mature B-cell lymphoid neoplasm 가천의대길병원진단검사의학과 안정열

Flow cytometric approach to the diagnosis and classification of mature B-cell lymphoid neoplasm 가천의대길병원진단검사의학과 안정열 Flow cytometric approach to the diagnosis and classification of mature B-cell lymphoid neoplasm 가천의대길병원진단검사의학과 안정열 Mature B-cell neoplasm 1. Mature B-cell : SIg(+) & lack of Ags of immaturity (TdT, CD34)

More information

HPV DNA Testing. The digene HPV Test. Clinical vs. Analytical Sensitivity. Sample & Assay Technologies

HPV DNA Testing. The digene HPV Test. Clinical vs. Analytical Sensitivity. Sample & Assay Technologies HPV DNA Testing The digene HPV Test Clinical vs. Analytical Sensitivity Sample & Assay Technologies How to validate an HPV test for optimal clinical performance Clinical sensitivity more accurately represents

More information

Assays to evaluate cell-mediated immunity. Guus Rimmelzwaan Department of Virology Erasmus Medical Center Rotterdam The Netherlands

Assays to evaluate cell-mediated immunity. Guus Rimmelzwaan Department of Virology Erasmus Medical Center Rotterdam The Netherlands Assays to evaluate cell-mediated immunity Guus Rimmelzwaan Department of Virology Erasmus Medical Center Rotterdam The Netherlands CBER/NIAID/WHO, Bethesda MD, December 11 2007 A working model of an antiviral

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

HBV screening and management in HIV-infected children and adolescents

HBV screening and management in HIV-infected children and adolescents HBV screening and management in HIV-infected children and adolescents Linda Aurpibul M.D. Research Institute for Health Sciences, Chiang Mai University 8% HIV and Hepatitis B Co-infection Among Perinatally

More information

Prospects for Vaccines against Hepatitis C Viruses. T. Jake Liang. M.D. Liver Diseases Branch NIDDK, NIH, HHS

Prospects for Vaccines against Hepatitis C Viruses. T. Jake Liang. M.D. Liver Diseases Branch NIDDK, NIH, HHS Prospects for Vaccines against Hepatitis C Viruses T. Jake Liang. M.D. Liver Diseases Branch NIDDK, NIH, HHS HCV Vaccine Prevention strategies Protective immunity Barriers and solutions Vaccine candidates

More information

DETERMINATION OF HER2 STATUS IN BREAST CANCER PATIENTS BY TWO METHODS: REAL-TIME QUANTITATIVE PCR (QPCR) AND IMMUNOENZIMATIC ASSAY (EIA)

DETERMINATION OF HER2 STATUS IN BREAST CANCER PATIENTS BY TWO METHODS: REAL-TIME QUANTITATIVE PCR (QPCR) AND IMMUNOENZIMATIC ASSAY (EIA) DETERMINATION OF HER2 STATUS IN BREAST CANCER PATIENTS BY TWO METHODS: REAL-TIME QUANTITATIVE PCR (QPCR) AND IMMUNOENZIMATIC ASSAY (EIA) Maria Savino, Paola Parrella, Massimiliano Copetti, Raffaela Barbano,

More information

Human CD4+T Cell Care Manual

Human CD4+T Cell Care Manual Human CD4+T Cell Care Manual INSTRUCTION MANUAL ZBM0067.02 SHIPPING CONDITIONS Human CD4+T Cells, cryopreserved Cryopreserved human CD4+T cells are shipped on dry ice and should be stored in liquid nitrogen

More information

Albumin. Prothrombin time. Total protein

Albumin. Prothrombin time. Total protein Hepatitis C Fact Sheet February 2016 www.hepatitis.va.gov Laboratory Tests and Hepatitis If you have hepatitis C, your doctor will use laboratory tests to about learn more about your individual hepatitis

More information

Pathology No: SHS-CASE No. Date of Procedure: Client Name Address

Pathology No: SHS-CASE No. Date of Procedure: Client Name Address TEL #: (650) 725-5604 FAX #: (650) 725-7409 Med. Rec. No.: Date of Procedure: Sex: A ge: Date Received: Date of Birth: Account No.: Physician(s): Client Name Address SPECIMEN SUBMITTED: LEFT PIC BONE MARROW,

More information

Molecular Diagnosis of Hepatitis B and Hepatitis D infections

Molecular Diagnosis of Hepatitis B and Hepatitis D infections Molecular Diagnosis of Hepatitis B and Hepatitis D infections Acute infection Detection of HBsAg in serum is a fundamental diagnostic marker of HBV infection HBsAg shows a strong correlation with HBV replication

More information

Pathogens and the immune system

Pathogens and the immune system Review of lecture 7 Pathogens and the immune system Veronica Leautaud, Ph.D. vl2@ rice.edu BRC 511 / 530-lab Lecture 8 BIOE 301-Bioengineering and World Health Science Science is the human activity of

More information

Article 53(c) EPC - therapy

Article 53(c) EPC - therapy Article 53(c) EPC - therapy Compound X is not new, but no medical use (a) (b) (c) (d) (e) (f) (g) (h) (i) (j) (k) compound X for use as a medicament compound X for use in treating disease Y composition

More information

EDF Extended Depth of Field

EDF Extended Depth of Field EDF Extended Depth of Field An upgrade for the ImageStream system Think outside the dot. Break the classic depth of field barrier Improve precision Enhance discrimination Simplify analysis Increase resolution

More information

Perspective Incomplete Viral Suppression Under Potent Antiretroviral Therapy: Determinants of Treatment Outcome

Perspective Incomplete Viral Suppression Under Potent Antiretroviral Therapy: Determinants of Treatment Outcome Perspective Incomplete Viral Suppression Under Potent Antiretroviral Therapy: Determinants of Treatment Outcome At the International AIDS Society USA course in Los Angeles in March 22, Steven G. Deeks,

More information

Need & the state of the art

Need & the state of the art Need & the state of the art Long-term treatment What problems in the antiretroviral therapy today? Compliance Adherence Toxicity Failure Resistance Andreoni November 2013 Antiretroviral agents in clinical

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

Potency Assays for an Autologous Active Immunotherapy (Sipuleucel-T) Pocheng Liu, Ph.D. Senior Scientist of Product Development Dendreon Corporation

Potency Assays for an Autologous Active Immunotherapy (Sipuleucel-T) Pocheng Liu, Ph.D. Senior Scientist of Product Development Dendreon Corporation Potency Assays for an Autologous Active Immunotherapy (Sipuleucel-T) Pocheng Liu, Ph.D. Senior Scientist of Product Development Dendreon Corporation Sipuleucel-T Manufacturing Process Day 1 Leukapheresis

More information

AP Biology Learning Objective Cards

AP Biology Learning Objective Cards 1.1 The student is able to convert a data set from a table of numbers that reflect a change in the genetic makeup of a population over time and to apply mathematical methods and conceptual understandings

More information

Understanding the role of ex vivo T cell depletion

Understanding the role of ex vivo T cell depletion Prevention of graftversus-host disease (GVHD) Understanding the role of ex vivo T cell depletion Information for patients undergoing allogeneic stem cell transplantation in AML and their families 2 This

More information

HIV-1 mother-to-child transmission and prevention: success and controversies

HIV-1 mother-to-child transmission and prevention: success and controversies 6 th Workshop on HIV Transmission Rome July 2011 HIV-1 mother-to-child transmission and prevention: success and controversies Gabriella Scarlatti San Raffaele Scientific Institute, Milan, Italy Transmission

More information