The comparison of scanner properties - the results of the 1 st European Scanner Contest
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1 The comparison of scanner properties - the results of the 1 st European Scanner Contest T. Schrader 1,2,K. Schlüns 2, N. Zerbe 2, M.G. Rojo 3, A. Laurinavicius 4, P. Hufnagl 2 1 University of Applied Sciences Brandenburg 2 Institut für Pathologie, Charité Universitätsmedizin Berlin 3 Servicio de Anatomia Patologica, Hospital General de Ciudad Real 4 National Centre of Pathology, Vilnius, Lithuania June 26, 2010
2 Outline 1 Background 2 Material & Methods 3 Results 4 Discussion 5 Summary Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
3 Background Technology & Consequences Scanning process Mosaic-scan Line-scan Autofocus Interpolation Dynamic focus Quality parameter Resolution Speed of scan Image sharpness Colour Contrast Time to present a slide with a viewer Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
4 Material & Methods Participating manufacturers Figure: Overview of manufacturers Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
5 Material & Methods Scanner Nanozoomer 2.0 HT (Hamamatsu) SCN SL801 (Leica) Pannoramic Scan 150 (3DHistech) Pannoramic Desk (3DHistech) Metafer 4 (MetaSystems) Metafer 4 + Loader (MetaSystems) D-Sight 05 (Menarini).slide + VC50 (Olympus) Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
6 Material & Methods Subjects of contest Mass-Scan 35 slides (15 Op, 20 Biopsies), en block, no interaction after start of scanning process allowed Quality-Scan 10 slides, interaction allowed after start of scanning process Cytology-Scan 12 cytology slides, interaction allowed Immun-Scan 11 IHC slides (Ki67, Her2neu u.a.) Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
7 Material & Methods Evaluated parameter Technical parameter preparation & scanning time correct scanned slides scanned area, missing areas file size area/time relation file size / scanned area Quality parameter Rank of manual evaluation Automatic evaluation of image sharpness Tennenbaum Laplace Wienert Unsharp areas Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
8 Material & Methods The beginning of contest - build up the systems Figure: MetaSystems in action Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
9 Material & Methods Briefing of referees Figure: Students of HTW & K.Saeger (VMScope) Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
10 Material & Methods Creation of screenshots for evaluation of quality Figure: Students of University of Applied Sciences Brandenburg Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
11 Results Optical resolution of scanners Hersteller 20x 40x 3DHistech 0,35 0,22 Hamamatsu 0,46 0,23 Leica 0,25 MetaSystems 0,32 0,26 Olympus 0,32 0,16 Menarini 0,5 0,25 Figure: Implications of different resulutions Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
12 Results Image quality example 1 Figure: HE-Slide Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
13 Results example 1 Results Figure: Comparison of image quality Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
14 Results Image quality example 2 Figure: IHC-Slide Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
15 Results example 2 Results Figure: IHC-Slide Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
16 Results Image quality example 3 Figure: Semi thin slide Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
17 Results example 3 Results Figure: Semi thin slide Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
18 Scanning time Results Figure: Mass scan Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
19 Discussion Limits of the first scanner contest Evalution of image quality Manual Automatic Comparability of resultion, used objective and monitor Comparability of viewer Current tested: light microscopy for routine, no flourescence No image analysis Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
20 Discussion Vilnius Conference 10 th European Congress on Telepathology and 4 th International Congress on Virtual Microscopy July, 1 st, 2010, 17:30 o clock Round table & Award ceremony of Scanner Contest Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
21 Summary Take home message First Scanner contest with comparable images and measurements Every participant will get a personal feedback with detailed information Website will be published soon. Next contest - next year Fluorescence Microscopy Additional quality parameter Image analysis Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
22 Summary Sponsors European Society of Pathology Deutsche Gesellschaft für Pathologie Bundesverband Deutscher Pathologen Barco (Monitors) COST Action IC 0604 WG 3, WG4 Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
23 Summary Resourcing of slides I. Anagnostopoulus (Berlin) H. Guski (Potdam) G. Haroske (Dresden) F. Heppner (Berlin) I. Petersen (Jena) A. Laurinavicius (Vilnius) H. Lobeck (Potsdam) C. Röcken (Kiel) J. Slodkowska (Warsaw) P. Ziemke (Potsdam) Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
24 Summary Organisation VMScope - K. Saeger, S. Wienert Charité - N. Zerbe, U. Brunner Hochschule Technik und Wirtschaft Fachhochschule Brandenburg Schrader et al. (Charité & FHB) Scanner Contest June 26, / 24
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