OxySense Gen III Operations Manual

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1 OxySense Gen III Operations Manual OxySense GEN III 300 Series OxySense GEN III 5000 Series COMPLIES WITH ASTM OxySense, Inc North Central Expressway, Suite 440 Dallas, Texas Phone Fax Gen III Version 4.0 For technical support with your OxySense System: Call : extension Web Site : Document Revised: February 12, 2010 Information in this document is subject to change without notice. Any reference to a company or data used as examples herein is fictitious unless otherwise noted. No part of this document may be reproduced or transmitted in any form or by any means for any purpose without express written permission. All other company and product names are the trademarks or registered trademarks of their respective companies. All rights reserved

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3 Page: 2 Table of Contents 1 Introduction to OxySense Gen III 4 2 Unpacking and Verification 6 1 Gen III 5000 Series Models 7 2 Gen III 300 Series Models 9 3 OxySense Gen III Operations 11 1 Background Correction 11 2 Main Screen 13 3 Configuration Screen & Activate Permeation 15 4 View Screen (Settings) 21 5 Logging / Timer Screen Adding a New Test Opening an Existing Test Browse Active Tests Browse All Tests View Log Start Timer Start Logging 38 6 Graph Screen 40 7 Calibration Screen 41 8 Diagnostics Screen 42 9 Error Log 46 4 Making Measurements Preparing the Package or Container to be Measured Preparing the System for Use Taking a Reading 49 5 Permeation Measurements Film Permeation Adding a New Chamber and New Test Name Adding a New Test for an Existing Chamber Opening an Existing Test Browse Active Tests Browse All Tests View Log File and Log Graph Setup (Setting) Timer Clicking the Timer button will start automatic capture for the selected test. The automatic capture will be performed after the specified interval of time End Test 65

4 Page: Print Report Capture Package Permeation Open Test Adding a New Test Browse Active Browse All View Log File and Log Graph Setup (Setting) Timer End Test Summary Report Print Report Capture 93 6 Contact Us 94 7 Appendix A Principles of Operation 95 A.1 Introduction 95 A.2 Oxygen Sensitive Coating (OxyDot ) 95 A.3 Dynamic Quenching Process 96 8 Appendix B User Calibration Appendix C Specifications Appendix D Warranty Information 103

5 Page: 4 1 Introduction to OxySense Gen III The OxySense Gen III Non-Invasive Oxygen Analyzer System is an optical measurement method for determining the Oxygen concentration in various media such as packages, headspace and liquid. The OxySense Gen III is available in two primary model series. The 300 series models utilize the user s PC to communicate with the Gen III instrument. The 5000 series models include an onboard PC to provide standalone measurements. The Gen III system can perform oxygen permeation testing of both packages and films, oxygen concentration studies in air or dissolved in liquid, shelf life studies and leak detection. The Oxygen concentration is non-invasively measured in packages and the measurement is reliable and repeatable. The OxySense GEN III systems comply with ASTM Fig. 1 The OxySense Gen III Non-Invasive Oxygen Analyzer Systems. The OxySense Gen III is ideally suited for use in R & D and quality assurance programs. It brings an easy, accurate, and low-cost method of conducting oxygen testing for modified atmosphere packaging (MAP), vacuum packaging, Oxygen scavenging, closure seal, PET bottle and product shelf-life studies.

6 Page: 5 The OxySense Gen III Non-Invasive Oxygen Analyzer System offers you the following benefits and features: Benefits: Non-destructive measuring technique Multiple measurements on the same package Fast measurement (5 seconds) Accuracy at ambient, 5% of the reading Reproducibility better than 1% (0.2% O 2) Sensor can withstand up to 150 C for 24 hours Can be used in oil, water and air Independent of ph (2-12) and salt concentration No interference from other gases Features: Gen III 5000 series Onboard PC Integrated Touch Screen Display Keyboard Mouse Built-in Hard drive (21 gigabytes) 6 USB Ports Automatic Temperature Measurement Automatic Barometric Measurement Data Logging Timer Function Integrated Report Writer Gen III 300 series Automatic Temperature Measurement Automatic Barometric Measurement Data Logging Timer Function Integrated Report Writer

7 Page: 6 2 Unpacking and Verification Upon receiving the OxySense Gen III system please ensure that you have received the following components: OxySense Gen III Instrument (5000 or 300 series) OxySense Gen III Operations Manual CD EasAlign reader-pen assembly with infrared detector Power Cable Flexible Keyboard (Gen III 5000 series models) Mouse (Gen III 5000 series models only) USB Mass Storage (Gen III 5000 series models only) Depending on your order, certain option items may also be included, such as: o OxyDot Application Kit o OxyDots If the unit you purchased is a 5000 Series, please proceed to Section 1 If the unit you purchased is a 300 Series, please proceed to Section 2

8 Page: 7 1 Gen III 5000 Series Models Please follow these instructions to ensure the 5000 series instrument functions properly once you have received it. Fig 1 OxySense Gen III 5000 Unpack the instrument and turn it around so that you are facing the back of the unit. Fig 2 The back of the OxySense 5000 Locate the power cord connection on the lower left of the back panel and insert the power cord. Turn the instrument around so that it now faces forward. Locate the fibre bundle and thermocouple connections on the faceplate (Fig. 3). Fig The fibre bundle and thermocouple connections Insert the metal fibre optic connector into the housing on the front panel. Rotate the nut by hand until snug. DO NOT USE PLIERS OR WRENCH TO TIGHTEN AS OVERTIGHTENING WILL DAMAGE THE CABLE. Connect the black temperature connector into its appropriate connection. Please note that there is an index notch to assist with proper alignment. A properly aligned connector will slide on easily. After it is in all the way, turn the locking collar by hand to the right to secure the connection 4. Connect the Keyboard to lower USB port located on the left side of the front panel Fig 4 (this is the Only USB port for the keyboard).

9 Page: 8 Fig 4. The keyboard connection The Mouse may be connected using any other available USB port (front panel, upper or any of the four on the back panel). If you are using a barcode scanner and/or print, printer, you may connect them to any other available USB ports. 5. Plug the instrument into a wall power outlet ( VAC 50/60 Hz). We strongly recommend you use a surge suppressor as the interface between the unit and the wall power. This will help you protect your units and prevent unexpected power related events. Push the button labeled Power located on the right face of the instrument to switch on the unit. A green light on the button should be lit when turned on. (When Power is successfully turned on, a blue light should appear at the end of the pen briefly and then disappear.) 6. Once the standard Windows desktop appears, double click the OxySense Gen III icon to launch the program. 7. Your instrument is equipped with the new EasAlign Pen with an integrated capture switch Fig 5. This makes taking a measurement easy and efficient. All you need to do to take a measurement is position the pen directly over the OxyDot and click the button. Fig 5. EasAlign pen with integrated capture switch 8. Now you are ready to startup your system and start testing. PLEASE PROCEED TO SECTION

10 Page: 9 2 Gen III 300 Series Models Please follow these instructions to ensure the 300 series instrument Fig 6 functions properly once you have received it. Fig 6 Gen III 300 series Install the Gen III Software as described in the Gen III Installation Guide. After the installation is complete, unpack the unit and turn it around so the back of the unit is facing you Fig 7. Fig 7 Back of the Gen III 300 series Locate the power cord connection on the upper right of the back panel and attach the power cord Fig 8. We strongly recommend that you plug the power cord into a surge suppressor and connect the surge suppressor to an available wall power outlet ( VAC 50/60 Hz). Locate the USB connection on the back panel and insert the square end of the USB cable. Attach the other end to the USB 0 port on your computer. Fig 8 Power cord attached to the 300 series 4. Turn the instrument around so that it now faces forward. Locate the fibre bundle (metal) and thermocouple (black) connections on the faceplate Fig 9. Fig 9 Gen III 300 series with fibre bundle

11 Page: Insert the metal fibre bundle connector into the housing on the front panel. Rotate the nut by hand until snug Fig 10. DO NOT USE PLIERS OR WRENCH TO TIGHTEN AS OVERTIGHTENING WILL DAMAGE THE CABLE. 6. Connect the black temperature connector into its appropriate connection. Please note that there is an index notch to assist with proper alignment. A properly aligned connector will slide on easily. After it is in all the way, turn the locking collar by hand to the right to secure the connection Fig 10 Gen III 300 series with fibre bundle and temperature sensor connected 7. Attach the USB cable to the back of the unit and the computer Fig 11 Fig 11 Gen III 300 series with USB connected 8. After completing the above step, power on the device. You will now be asked to install the software driver for Gen III. Refer to the Gen III Installation Guide section 4.0 Driver Installation to install the Gen III driver. 9. After the driver installation is complete, start the Gen III by clicking the OxySense Gen III v1 Icon in the Desktop or go to Start -> Program -> OxySense Gen III and click OxySense Gen III v Your instrument is equipped with the new EasAlign Pen with an integrated capture switch Fig 1 This makes taking a measurement easy and efficient. All you need to do to take a measurement is position the pen directly over the OxyDot and click the button. Fig 12 EasAlign Pen with integrated capture switch You are now ready to proceed to Chapter 3 Operation of the OxySense Gen III.

12 Page: 11 3 OxySense Gen III Operations Please note that the system date will have Day, Month and Year and the Time will have hour, minute and second. If the System Date format does not have all required Day, Month or Year components defined and System Time format does not have all required hour, minute or second components defined, the user can set the date and time format in the following manner: Date: The Date format can be changed by navigating to Window s Control Panel >> Regional and Language Option >> Regional Options >> Customize >> Date. Time: The Time format can be changed by navigating to Window s Control Panel >> Regional and Language Option >> Regional Options >> Customize >> Time. While executing the OxySense Gen III software, if the OxySense Gen III device is not connected, the software will display the following message with three options: 1) Retry 2) Continue 3) Abort. 1) Retry: Clicking this button will perform a rescan for the OxySense Gen III device. 2) Continue: Clicking this button will load the software without the OxySense Gen III device. 3) Abort: Clicking this button will end the execution of the software. 1 Background Correction For the best accuracy, a background correction is needed, especially when the signal level is low (20% of maximum signal level). The Background Correction is a reading taken first without the OxyDot followed by a reading with an OxyDot. The GEN III software automatically calculates the background correction. The background correction is only performed when the Gen III software is started. When the application starts, the following screen will be displayed within the Main screen:

13 Page: 12 Capture Fig. 1: Background Correction Screen Click the Capture button while pointing the Pen away from the OxyDot. Clicking the Capture button will close the Background Correction window and the following screen will be displayed: Capture Fig. 2: Background Correction Screen Click the Capture button while pointing the Pen at the OxyDot. Clicking the Capture button will close the Capture Oxygen window and the Main screen (Fig. 3) will be displayed with an Oxygen reading.

14 Page: 13 2 Main Screen (If the Permeation feature is enabled, the Gen III-P logo will be displayed on the Top. If the Permeation feature is not enabled, only the Gen III logo will be displayed on the Top (shown below).) Oxygen Measurement Temperature Calibration da/db 4. Logging 5. Timer 6. Pressure 7. View 8. Config 9. Capture 10. Status Message Fig Main Screen-1: Main Screen 1 On-Screen Keyboard 1 About Measured Oxygen Displays the Oxygen concentration measured in the units chosen in the View screen (%, mbar or ppb). Temperature The temperature reading is taken automatically via the infrared sensor built into the reader pen. However, if you wish to enter the temperature manually, it is possible to do so in the View screen with an accuracy of one-tenth of a degree. Calibration da/db The OxyDot calibration da/db numbers being used for the measurement. 4. Logging Button and Indicator The Logging button activates the Logging function and changes the Logging indicator from red to green. This feature enables the logging of real-time Oxygen measurement data for a specified Test. Click the Logging/Timer tab of the Main menu screen. Enter or select the Test Name with Sample Number in the Test Name field through Barcode, Keyboard or the Browse Active/All window. If you do not specify a Test Name

15 Page: 14 with Sample Number in the Test Name field of the Logging/Timer menu, the logging function cannot be enabled. After specifying the Test Name with Sample Number, click the Logging button. The Logging function will begin and the Logging indicator will be displayed in green. The specified Test Name with Sample Number will be displayed below the Menu 5. Timer Button and Indicator The Timer button activates the Timer function for automated measurements and changes the Timer indicator from red to green. Click the Logging/Timer tab of the Main menu screen. Locate the field on the left of the screen labeled Measurement Interval and enter the desired measurement intervals in seconds or minutes. The minimum time for a single reading is 1 second. Therefore, the minimum timer is 1 second. The initial default value is 5 seconds. After completing Step 1 above, click the Timer button to begin the measurements with the specified interval and the indicator will change from red to green. The Timer function can be cancelled by clicking the Timer button and the indicator will be changed to red. Note: Clicking the Timer button from any menu screen without setting the desired interval will default to the interval currently specified in the Measurement Interval field of the Logging/Timer menu. 6. Pressure The barometric pressure is measured automatically via a sensor built into the processor board. However, if you wish to enter the pressure manually, it is possible to do so in the View screen. 7. View Clicking the View button will open a new window to change the settings for Oxygen Reading Unit of Measure, Temperature, Calibration and Pressure. 8. Config Clicking the Config button will open a window to Lock/Unlock the Menus, Lock/Unlock the View window, Change Language, Serial Number, specify the Alert High/Low Oxygen and Activate the Permeation features. 9. Capture Clicking the capture button performs an oxygen measurement. If the Test Name with Sample Number has been selected from the Logging/Timer menu, clicking the Capture button will capture a new Oxygen reading, update the Log, update the Graph and save the Data Point in the database. If no Test has been specified, the new Oxygen reading will be displayed, the Graph will be updated but the data point will not be saved. 10. Status Message Warning or Error message related to the Oxygen measurement will be displayed in this field. 1 On-Screen Keyboard Clicking the On-Screen Keyboard button will open the On-Screen Keyboard.

16 Page: 15 1 About Clicking the? button in the Title Bar will display the following screen. This screen displays the currently installed OxySense Gen III software version. 3 Configuration Screen & Activate Permeation To open the configuration window, click the Config button in the Main screen. If the Configuration is locked, the button will be displayed as follows: If the Configuration is locked and you click the above Config button, a new window will be opened to enter the Password. a. Password b. Change Password c. Cancel d. OK Fig 4. Password-1: Configuration Password Screen. a. Password: Enter the Password in the Password field. The factory set password is oxy. b. Change Password: To change the Password, click the Change Password label to display the following screen:

17 Page: 16 a. Old Password b. New Password c. Confirm Password d. OK e. Cancel Fig 5. Password-2: Configuration Change Password Screen. a. Old Password: Enter the old Password in the Old Password field. b. New Password: Enter the new Password in the New Password field. c. Confirm Password: Enter the identical new Password in the Confirm Password field. d. Update: Clicking the Update button will update the system with the new Password. If the Old Password matches and the New Password and Confirm Password are identical, a message box will display Password Updated Successfully!. If the Old Password does not match the database entry, an error message will be displayed. If the New Password and Confirm Password are not identical, an error message will be displayed. e. Cancel: Clicking Cancel button closes the Configuration Password window. c. Cancel: Clicking Cancel button closes the Configuration Password window. d. OK: After entering the correct Password, clicking the OK button will close the Configuration Password window and display the Configuration window. You can change the Lock/Unlock Status of various elements of the system, Activate the Permeation features, specify the Language and set the Oxygen Low/High values.

18 Page: 17 Configuration View Activate Permeation 4. Tab Screens 5. Serial Number 6. Language 7. Enable Oxygen Alert 8. Oxygen Alert Low and High 9. OK 10. Diagnostic 1 Cancel Fig.6 Configuration -1: Configuration Screen Configuration You can change the Lock/Unlock status of the Configuration screen. If you select the Locked radio button and click the OK button, the Configuration window will be locked and require a password to open in the future. If you select the Unlock button and click the OK button, the Configuration window will be unlocked. The Config button image will be changed in the Main screen to the following to indicate a Password is not required to open the Configuration window. View You can change the Lock/Unlock status of the View screen. If the View is unlocked, the settings within the View window can be changed. If the View is locked, the settings will be visible in the View window but cannot be changed. Activate Permeation (For the 5000 series, the permeation software will be activated by the factory. For the 300 series, the permeation software is an option. If you have purchased the permeation software option you will need to proceed as described below). When you click the Activate Permeation button, the following screen will be displayed with the Registration Key for activating the Permeation functions.

19 Page: 18 a. Registration Key b. License Key field c. OK d. Cancel Fig 7. Configuration -3: Permeation Registration a. Registration Key: The Registration Key must be provided to OxySense by phone or to receive the License Key for activating the Permeation. OxySense will generate the License Key for the Registration Key provided by you and return the License Key to you through phone or . b. License Key Field: After receiving the License Key from OxySense. The License Key must be entered in the License Key field of the Permeation Registration window as displayed below. Fig8 Configuration -4: Permeation Registration with License Key c. OK: After entering the License Key in the button for verification. If the License Key closed and Permeation Tab can be viewed The Gen III Logo will be replaced with Permeation feature is enabled. d. Cancel: Clicking the Cancel button will close the Registration Key window and the Activate Permeation registration will be canceled and the Logo will not be replaced. License Key field, click the OK is accepted, the window will be in the screen for Lock/Unlock. the Gen III-P Logo when the

20 Page: Tab Screen The Tab screen allows you to Lock/Unlock the various tabs within the Main menu (Logging/Timer, Graph, Film/Package Permeation (if Permeation is Active), Calibration and Diagnostics). If the function is locked, the tab will not be visible on the Main menu screen and therefore the function is effectively disabled. a. Logging/ Timer Fig 9. Configuration -3: Configuration Tab Screen 5. b. Graph c. Permeation d. Calibration e. Diagnostics a. Logging/Timer: If you select the Locked radio button, the Logging/Timer tab will not be displayed in the Main window. If the Unlocked radio button is selected, the Logging/Timer tab will be displayed and the function enabled. b. Graph: If you select the Locked radio button, the Graph tab will not be displayed in the Main window. If the Unlocked radio button is selected, the Graph tab will be displayed and the function enabled. c. Permeation: If the Permeation is Active, this section will be visible else this section will not be displayed. If you select the Locked radio button, the Film/Package Permeation tab will not be displayed in the Main window. If the Unlocked radio button is selected, Film/Package Permeation tab will be displayed and the function enabled. d. Calibration: If you select the Locked radio button, the Calibration tab will not be displayed in the Main window. If the Unlocked radio button is selected, the Calibration tab will be displayed and the function enabled. e. Diagnostics: If you select the Locked radio button, the Diagnostics tab will not be displayed in the Main window. If the Unlocked radio button is selected, the Diagnostics tab will be displayed and the function enabled. Serial Number Display the Gen III Serial Number entered during the installation of the software (300 series only). The Serial Number can be replaced if a replacement unit is provided in the future. If you enter an incorrect Serial Number in the Serial Number field, an error message will be displayed: Invalid Serial Number. 6. Language You can select the Language from the combination drop down box to display the screen labels and messages in the desired language. The default language is English. Currently, English is the only language available. 7. Enable Oxygen Alert You can specify if the Alarm should be enabled when the Oxygen Measurement is beyond the range specified in the High and Low Oxygen Alert fields. If the Enable Oxygen Alert check box is checked, it will enable the Alarm. If the Oxygen Measurement is outside the range specified in the Oxygen High and Low Alert fields, the measurement will be displayed in Red color with an alarm.

21 Page: Oxygen Alert Low and High High You can specify the Oxygen High Alert level to display an Audio Visual Alarm and display the Oxygen reading in red if the Oxygen measurement exceeds the High Alert Level. Low You can specify the Oxygen Low Alert level to display an Audio Visual Alarm and display the Oxygen reading in red if the Oxygen measurement is less than the Low Alert level. 9. OK If you change any of the elements within the Configuration screen, click the OK button to save the changes in the registry and reflect the changes in the Main window. 10. Diagnostic At the time of executing the application, if an error occurs, while communicating with the Gen III device, the error messages can be logged by turning on the Diagnostics by checking the Turn ON Diagnostics check box. 1 a. If the Turn ON Diagnostics check box is unchecked, it will not log the messages. b. If the Turn ON Diagnostics check box is checked, it will log the messages if an error occurs while communicating with the Gen III device in the log file COMPORT_Log.txt and DG_Log.txt, which will be stored at the following folder path: GENIII/COMPORT. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the above log files will be created at: C:\GENIII\COMPORT. Cancel If you do not want to save the changes within the Configuration screen, click the Cancel button.

22 Page: 21 4 View Screen (Settings) Oxygen View By Temperature Celsius/Fahrenheit 4. Default Temperature 5. Pressure 6. Default Pressure 7. OxyDot Calibration 8. OK 9. Cancel Fig 10. View-1: View Screen Oxygen View By This area allows you to choose which unit of measure to display the Oxygen measurement. The measured Oxygen concentration can be displayed in: a. Percent Percentage of Oxygen by volume including headspace measurements. If the Percent View By is selected, you may select the number of decimal places to display. 1 Decimal: If the 1 Decimal radio button is selected, a single digit will be displayed after the decimal point. 2 Decimal: If the 2 Decimal radio button is selected, two digits will be displayed after the decimal point. b. PPB To view the Dissolved Oxygen (DO) in parts per billion when the OxyDot is in equilibrium with the liquid. c. mbar To view the Oxygen partial pressure in millibars including the headspace. Temperature This area allows you to Hide or Display the Temperature in the Main screen. If the Display radio button is selected, the Temperature will be displayed in the Main screen. If the Hide radio button is selected, the Temperature will not be displayed in the Main screen.

23 Page: 22 Celsius/Fahrenheit This area allows you to select the Temperature display in Celsius or Fahrenheit in the Main screen. 4. If the Celsius radio button is selected, the Temperature will be displayed in Celsius in the Main screen. If the Fahrenheit radio button is selected, the Temperature will be displayed in Fahrenheit in the Main screen. Default Temperature This area allows you to enter a Default Temperature and select if the Default or Probe Temperature will be displayed in the Main screen and be utilized in the Oxygen calculation. 5. Enter the Default Temperature value in the Default Temperature field. If the Always Use Default Temperature check box is selected, the Oxygen calculation will utilize the Default Temperature value and the Default Temperature will be displayed in the Main screen. If the Always Use Probe Temperature check box is selected, the Oxygen calculation will utilize the real time Temperature value from the Gen III device and the Probe Temperature will be displayed in the Main screen. Pressure This area allows you to Hide or Display the Pressure in the Main screen. 6. If the Display radio button is selected, the Pressure will be displayed in the Main screen. If the Hide radio button is selected, the Pressure will not be displayed in the Main screen. Default Pressure This area allows you to enter the Default Pressure and select if the Default or Instrument Pressure will be utilized for displaying in the Main screen and be utilized in the Oxygen calculation. 7. Enter the Default Pressure value in the Default Pressure field. If the Always Use Default Pressure check box is selected, the Oxygen calculation will utilize the Default Pressure value and the Default Pressure value will be displayed in the Main screen. If the Always Use Instrument Pressure check box is selected, the Oxygen calculation will utilize the real time Pressure value from the Gen III device and that Pressure will be displayed in the Main screen. OxyDot Calibration This area allows you to Hide or Display the OxyDot Calibration in the Main screen. If the Display radio button is selected, the OxyDot Calibration will be displayed in the Main screen. If the Hide radio button is selected, the OxyDot Calibration will not be displayed in the Main screen.

24 Page: OK If you change any of elements within the View screen, click the OK button to save the changes in the registry and reflect the changes in the Main window. 9. Cancel If you do not want to save the changes within the View screen, click the Cancel button.

25 Page: 24 5 Logging / Timer Screen New Test Test Name Browse Active 4. Browse All 5. View Log 6. Start Timer 7. Start Logging a. Interval b. Study Fig.11 Logging-1: Logging Screen 5.1 Adding a New Test To add a new Test with Samples, click the New Test button to display the following Add New Test (Logging) window. a. Test Name b. Test Description c. Number of Samples d. Unique Sample Description Fig.12 Logging-2: Logging Add New Test Screen a. e. Create All Bar Codes f. Add g. Cancel Test Name: Enter the New Test Name in the Test Name field. The Test Name field cannot be blank.

26 Page: 25 b. Test Description: Enter the Test Description in the Test Description field. The Test Description field is optional. c. Number of Samples: Enter the Number for Samples field to specify how many Samples will be utilized for the Test. The Number of Samples field cannot be blank. d. Unique Sample Description: Select Yes / No from the Unique Sample Descriptions combination drop down box. If No is selected, the Sample Description will be blank for all of the Samples. e. Create All Bar Codes: After entering the required information (Test Name, Description, Number for Sample and Unique Sample Description), click the Create All Bar Codes button to create all of the Barcode labels for the Samples. Clicking the Create All Bar Codes button will open the following Barcode window if the Number of Samples = 1 or the Unique Sample Descriptions = No. If the Number of Samples is > 1 and the Unique Sample Descriptions = Yes, the following message will be displayed: Are you sure you want to print all of the Bar Codes prior to entering the Unique Samples Descriptions?. If the Yes button is clicked, the Barcode window will be displayed. If the No button is clicked in the message box, the Unique Sample Description screen to enter the Unique Sample Descriptions for all of the Samples will be displayed. Width Height Top Margin 4. Left Margin 5. Orientation 6. Print Preview 7. Print 8. Close Fig 1 Logging-3: Logging Barcode Screen If you have not changed the Barcode labels from the labels delivered, the defaults are pre-filled and you may go directly to Print or Print Preview. Width: Enter the width in the Width field in inches for printing specific Barcode Label width. Height: Enter the height in the Height field in inches for printing specific Barcode Label height. Top Margin: Enter the top margin in the Top Margin field in inches for printing the Barcode Label.

27 Page: Left Margin: Enter left margin in the Left Margin field in inches for printing the Barcode Label. 5. Orientation: Select the Horizontal or Vertical radio button to control the printing Orientation of the Barcode Label. 6. Print Preview: Click the Print Preview button to view how the Barcode Label will be printed. f. 7. Print: Print the Barcode Labels. 8. Close: Close the Barcode window. Add Button Processing: After entering the Test Name, Volume, Test Description, Number of Samples and Unique Sample Descriptions (Yes/No); click the Add button to add the New Test Name with the Number of Samples to the database. Clicking the Add button will check whether the Test Name entered is already in the database. If the Test Name is found, an error message will be displayed: Test Name already exists. A duplicate Test Name is not allowed. If the Test Name field is blank, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name. If the Test Name entered is correct, the application will check the Number of Samples. If the Number of Sample field is 0, an error message will be displayed: Number of Samples should be greater than Please type proper Sample Number. If the Test Name and Sample Number is correct, the application will display the following confirm existing OxyDot Calibration message: The Current OxyDot Calibration is da=13 and db=0.0. Is the existing OxyDot Calibration correct? with Yes and No buttons. a. Yes : If you click the Yes button and if Yes is selected from the Unique Sample Descriptions combination drop down box, the Add New Test will be changed to Fig. Logging-5 to enter the Sample Description. If No is selected from the Unique Sample Descriptions combination drop down box, the Add New Test window will be closed and the New Test Name with the Sample Number will be opened in the Logging/Timer tab with the OxyDot calibration of da=13 and db=0.0. b. No : if you click the No button, the Add New Test window will be changed to the following screen to allow you to add unique OxyDot Calibration Numbers for each of the Samples:

28 Page: 27 OxyDot Calibration Confirm OxyDot Calibration Sample Number 4. OK 5. Cancel Fig.14 Logging-4: Logging OxyDot Calibration Screen OxyDot Calibration: Enter the OxyDot Calibration da and db Calibration fields. If the Calibration da field is blank, the application will display the following error message: Enter the valid value for the Calibration da. If the Calibration db field is blank, the application will display the following error message: Enter the valid value for the Calibration db. Confirm OxyDot Calibration: Enter the same OxyDot Calibration da and db fields in the Confirm OxyDot Calibration fields. If the Calibration da and confirm Calibration da does not match, the following error message will be displayed: The Calibration da and Confirm Calibration da does not match. If the Calibration db and confirm Calibration db does not match, the following error message will be displayed: The Calibration db and Confirm Calibration db do not match. Sample Number: This allows you to utilize the same OxyDot Calibration (da/db) for a range of Samples. The range must be within the total number of samples or an error will be displayed. 4. OK: Clicking the OK button will add the Calibration (da/db) for the selected Samples in the database. When the Calibration (da/db) for all of the Samples have been entered, the OxyDot Calibration window will be changed to the following screen to allow you to add unique Sample Descriptions if the Unique Sample Descriptions = Yes. If the Unique Sample Descriptions = No the Add New Test Window will be closed and the Test Name with Sample 1 will be Open in the Logging screen.

29 Page: 28 a. Sample Description b. Create Test Sample Barcode c. Next d. Close Fig 15. Logging-5: Logging Add Sample Description Screen a. Sample Description: The Sample Description box will allow you to sequentially enter the Unique Sample Description for each Sample Number. Enter the description in the Sample Description field and click the Next button to go to the Next Sample Number to enter its Sample Description. When the Unique Sample Descriptions have been entered for all of the Samples, the message box: Do you want to print the Bar Codes for all of the Samples? will be displayed. If the Yes button is clicked, the Barcode screen (Fig. Logging-3) will be displayed. The Print button will print all of the Barcodes for all of the Samples. Clicking the No button will close the Add Sample window and return to the Logging/Timer screen with the Test Name and Sample 1 displayed. b. Create Test Sample Barcode: To Create the Barcode for the specific Sample, click the Create Test Sample Barcode button to print the Barcode for each Sample, one at a time, while entering the Unique Sample Description. c. Next: Clicking the Next button will display the next Sample to enter the Unique Sample Description. d. Close: Clicking the Close button will close the Add New Test window and Open the Test Name with Sample 1 in the Logging/Timer screen. 5. g. Cancel: Clicking the Cancel button will display the (Fig. Logging-2) screen. Cancel: Clicking the Cancel button will close the Add New Test window.

30 Page: Opening an Existing Test Enter the Test Name with Sample Number in the Logging Test Name field (by scanning the Barcode or entering the name through the Keyboard). If the Test Name with Sample Number is entered through the Keyboard, press Enter button after entering the Test Name with Sample Number. If the Test Name with Sample Number is correct, the following Logging/Timer screen will be displayed. If the entered Test Name with Sample Number is not correct, the following error message will be displayed: Could not find the Test Name and Sample Number. Please type correct Test Name with Sample Number.. a. Display Test Name b. Oxygen Reading c. Sample Description d. Temperature Fig.16 Logging-6: Logging Screen with Test Name & Sample a. The Test Name with Sample Number will be displayed at the top of the screen. b. Displays Oxygen concentration measured in the units chosen in the View window. c. Displays the Sample Description for the Test Name with Sample Number. d. Displays the Temperature as per the selection in the View Window. 5.3 Browse Active Tests Clicking the Browse Active button displays the following window containing a list of the Open (Active) Tests (Test Name with Sample Number and Open Date & Time) for Logging. Fig 17. Logging-7: Logging Browse Active Tests Open Test Update Description Update Calibration 4. End Test 5. Print Barcode 6. Export File 7. Close

31 Page: 30 Open Test: To Open a Test, select the Test from the Open Tests list and click the Open Test button. Clicking the Open Test button closes the Browse Active window and displays the selected Test Name with Sample number in the Logging/Timer screen. Clicking the Open Test button without previously selecting a Test will display the following error: Please select the Test from the Open Test List to open the test.. Update Description: Clicking the Update Description button displays the selected Test Name with Sample Number in a new window with Test Description and Sample Description in the following screen to allow you to update the Description and Sample Description field in the database: a. Test Name b. Test Description c. Sample Description d. Update Description e. Cancel Fig.18 Logging-8: Logging Update Description a. Selected Test Name with Sample Number will be displayed. b. Update the Test Description in the Test Description field. c. Update the Sample Description in the Sample Description field. d. Click the Update Description button to update the Test Description and close the window. e. Click the Cancel button to close the window without updating the Descriptions. Update Calibration: Clicking the Update Calibration button displays the selected Test in a new window with Test Name, OxyDot Calibration and the Sample Number in the following screen to allow you to update the Calibration. If the Calibration da/db is changed, the previous data points for the Selected Test and the selected Samples will be recalculated as per the New Calibration da/db.

32 Page: 31 a. Test Name b. OxyDot Calibration c. Sample Number d. Update Calibration e. Cancel Fig.19 Logging-9: Logging Update Calibration (da/db) a. The Test Name with Sample Number will be displayed. b. Enter the New OxyDot Calibration da and db in the Calibration fields. If the Calibration da/db field is empty, an error message will be displayed. c. Specify the selected Sample range to update the OxyDot Calibration (da/db). The range must be within the number of Samples or an error message will be displayed. d. Clicking the Update Calibration button it will ask you for confirmation: Are you sure you want to update Calibration da and db. If Yes, the previous Data Points will be recalculated for the selected Samples. If you click the Yes button, the previous data points will be recalculated with the updated da and db for the Samples Numbers entered in From field to To fields. If you click the No button, the Calibration da and db will not be updated. e. Clicking the Cancel button will close the Update Calibration window without making any changes. 4. End Test: Please note that once you have ended a Test, you can no longer add additional readings to that Test. To End a Test, select the Test from the Active Test List and click the End Test button. Clicking the End Test button will display a message box: Are you sure you want to End the Test? You will not be able to Capture additional data points for an ended Test.. Clicking the OK button within the message box will display another message box with: End All Samples within the Test? For the Test. Clicking the Yes button from the second message box will End all samples within the selected Test Name and remove the selected Test and all the Samples from the Open Tests list. Clicking the No button will only End the selected Test Name with Sample Number and remove only that specific Test and Sample from the Open Tests list.

33 Page: 32 Clicking the End Test button without previously selecting a Test will display the following error message: Please select the Test from the Open Tests List to end the test. 5. Print Barcode: Clicking the Print Barcode button will determine if the selected Test Name has multiple samples. If the test has more than one sample, a message box will be displayed: Do you want to reprint the Barcode for the Test:? with All Samples, Selected Sample and No buttons. If you click the All Samples button, the Barcode window will be opened to allow you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a new window and display a print preview window with the Test Name and the last Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and all of the Sample Numbers. Close: Clicking the Close button will close the Barcode window. If you click the Selected Samples button, the Barcode window will be opened to allow you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a new window and display a print preview window with the Test Name and selected Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and selected Sample Number. Close: Clicking close button will close the Barcode window. If you click the No button, the Print Barcode message box will be closed. If the test has one sample, a message box will be displayed: Do you want to reprint the Barcode for the Test :? with Yes and No buttons. If you click the Yes button, the Barcode window will be opened to allow you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a print preview window with the Test Name and Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and Sample Number.

34 Page: 33 Close: Clicking the Close button will close the Barcode window. If you click the No button, the Print Barcode message box will be closed. 6. Export File: Clicking the Export File button will display a message box: Are you sure you want to export the CSV file for the Test:? with Yes and No buttons. If you click the Yes button, the application will check if the selected Test has more than one sample. If the Test has more than one sample, a second message box will be displayed: Do you want to export all Samples for the Test:?. If you click the Yes button from the second message box, all Samples for the Test will be exported in CSV format with an individual file for each Sample. If you click the No button from the second message box, only the selected Test with Sample Number will be exported in CSV format. The file name will be Test Name plus Sample Number plus Date & Time and will be saved at the following folder path: GENIII\LoggingReport\Logging folder. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in the C: drive, the path will be: C:\GENIII\LoggingReport. A message box will be displayed with the file name export path. Clicking the Export File button without previously selecting a Test with Sample Number will display the following error message: Please select the Test from the Open Tests List to export. 7. Close: Closes the Browse Active window.

35 Page: Browse All Tests Clicking the Browse All button displays the following window containing a list of Open (Active) Tests (Test Name and Open Date & Time) followed by all Closed Tests (Test Name, Open Date & Time and Close Date & Time). Open Test Update Description Update Calibration 4. End Test 5. Delete Test 6. Export File 7. Close Fig.20 Logging-10: Logging Browse All Tests Open Test: To Open a Test, select the Test from the Open Tests list or Ended Tests list and click the Open Test button. Clicking the Open Test button closes the Browse All window and displays the selected Test Name with Sample Number in the Logging/Timer screen. Clicking the Open Test button without previously selecting a Test with Sample Number will display the following error message: Please select the Test from the Open Tests List or the Ended Tests List to open the test. Update Description: Clicking the Update Description button displays the selected Test Name with Sample Number in a new window as per Fig. Logging-8 with the Test Description and Sample Description to allow you to update the Description. You are allowed to update the Test Description and Sample Description if the selected Test and Sample Number is ended. Update Calibration: Clicking the Update Calibration button displays the selected Test in a new window as per figure Fig. Logging-9 with Test Name, OxyDot Calibration and the Sample Number to allow you to update the Calibration. However, if the Calibration da/db is changed, the previous data points for the Selected Test and the selected Samples will be recalculated as per the New Calibration da/db. The Calibration for the Ended Test cannot be updated. 4. End Test: Please note that once you have Ended a Test, you can no longer add additional readings to that Test.

36 Page: 35 To End a Test, select the Test from the Active Test List and click the End Test button. Clicking the End Test button will display the following message: Are you sure you want to End the Test? You will not be able to Capture additional data points for an ended test. Clicking the OK button within the message box will display the following message: End All Samples within the Test? For the Test:. Clicking the Yes button from the second message box will End all Samples within the selected Test Name and remove the selected Test and all the Samples from the Open Tests list. Clicking the No button will only End the selected Test Name with Sample Number and remove only that specific Test and Sample from the Open Tests list. Clicking the End Test button without previously selecting a Test from the Active Test List will display the following error message: Please select the Test from the Open Tests List to end the test.. 5. Delete Test: To Delete a Test, select the Test from the Ended Tests list and click the Delete Test button. Clicking the Delete Test button will display the following message: Are you sure you want to Delete the Test?. Clicking the OK button within the message box will display the following message: Delete All Samples within the Test?. Clicking the Yes button from the second message box will delete all Samples within the selected Test Name and remove from the Ended Tests list. Clicking the No button will delete only the selected Test Name and Sample number and remove it from the Ended Tests list. Clicking the Delete Test button without previously selecting a Test from the Ended Test List will display the following error message: Please select the Test from the Ended Test List to delete the test.. 6. Export File: Clicking the Export File button will display a message box: Are you sure you want to export the CSV file for the Test :? with Yes and No buttons. If you click the Yes button, the application will check if the selected Test has more than one Sample. If the Test has more than one Sample, a second message box will be displayed: Do you want to export all Samples for the Test?. If you click the Yes button from the second message box, all Samples for the Test will be exported in CSV format with an individual file for each Sample. If you click the No button from the second message box, the selected Test with Sample Number will be exported in CSV format. The file name will be Test Name plus Sample Number plus Date & Time and will be saved at the following folder path: GENIII\LoggingReport\Logging folder. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the path will be: C:\GENIII\LoggingReport. A message box will be displayed with the file name export path.

37 Page: 36 Clicking the Export File button without previously selecting a Test with Sample Number will display the following error message: Please select the Test from the Open Tests List or the Ended Tests List to export. 7. Close: Closes the Browse All window. 5.5 View Log Clicking the View Log button displays the following Logging Report window containing the data points for the selected Test and Sample Number. If the Test Name with Sample Number is correct, the following Logging Report window will be displayed. If the entered Test Name with Sample Number is not correct, an error message will be displayed: Could not find the Test Name and Sample Number. Please type correct Test Name with Sample Number. You can sequentially view the Samples with the Next and Previous buttons and export the data pints in CSV or PDF format. Export to CSV PDF Report Next / Previous 4. Close Fig.21 Logging-11: Logging View Log Export to CSV: Clicking the Export to CSV button will display a message box: Are you sure you want to export the CSV file for the Test:? with Yes and No buttons. If you click the Yes button, the application will check if the selected Test has more than one Sample. If the Test has more than one Sample, a second message box will be displayed: Do you want to export all Samples for the Test?. If you click the Yes button from the second message box, all of the Samples and their respective data points for the test will be exported in CSV format with an individual file for each Sample. If you click the No button from the second

38 Page: 37 message box, the selected Test with Sample Number will be exported in a CSV format. The file name will be Test Name plus Sample Number plus Date & Time and it will be saved at the following folder path: GENIII\LoggingReport\Logging folder. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the above log files will be created at: C:\GENIII\COMPORT. A message box will be displayed with the file name export path. PDF Report: Clicking the PDF Report button will crate a PDF report for the selected Sample and it will be saved for the Test with the selected Test Name, Sample Number and Date & Time as the file name at the following folder path: GENIII\LoggingReport\Logging. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the above log files will be created at: C:\GENIII\COMPORT. A message box will be displayed with the file name and path. Next / Previous Next: If the Selected Test has more than one Sample, the Next >> button will be displayed to allow you to sequentially view the Sample details and data points. The Next >> button will not be displayed when the last sample is selected. Previous: If the Selected Test has more than one sample, the << Previous button will be displayed to allow you to view a prior Sample. The << Previous button will not be displayed when the first Sample is selected. View Log window Data a. Test Name with Sample Number b. Test Name c. Selected Test Description d. Sample Number e. Selected Sample Number Description f. Sr. No. (Serial Number) g. Data Points Capture Date & Time h. Oxygen in Percent i. Oxygen in mbar j. Oxygen in PPB k. Ratio

39 Page: 38 l. Temperature m. Pressure n. da o. db 5.6 Start Timer This button activates the Timer function. When the Start Timer button is clicked the button name be changed to Stop Timer and the Timer LED will be displayed in green for automated measurements. To enable automated measurements, the following steps are required: a. Measurement Interval: You must enter the interval in the Measurement Interval field in either Seconds or Minutes. The Minutes/Seconds can be selected from the Measurement Interval combination drop down box. The minimum time for a single reading is 1 second. Therefore, the minimum interval can be set to 1 second. If the desired time between readings is greater than 1 second, enter the number of Minutes/Seconds desired. After entering the interval Measurement Interval field, click the Start Timer button. The automated measurements will process continuously as per the interval specified in the Timer until you click the Stop Timer button. b. Study: Allows you to specify the number of readings to be taken by the Timer function. Enter the specific number of readings to be taken in the Readings in Study field. The readings will be taken in time increments as specified in the Time Between Readings field. After completing Step 1, select the Perform a Study check box to activate the Study. Readings will not begin until the Start Timer button is clicked. Once the readings have begun, they will be taken in increments as specified and until the indicated number of readings in the Readings in Study field has been achieved. 5.7 Start Logging This button activates the Logging function. When the Start Logging button is clicked, the button name will be changed to Stop Logging and the Logging LED will be displayed in green. This feature enables the logging of real-time Oxygen measurement data for the specified Test Name with Sample Number.

40 Page: 39 Specify the Test Name with Sample Number in the Test Name field through Barcode, Keyboard or through the Browse Active/All window. If you do not select a Test Name with Sample Number, the Logging function cannot be enabled. After specifying the Test Name with Sample number, click the Start Logging button and the button name will change to Stop Logging and the Logging LED will be displayed in green. If the Logging Function is ON, the data points will be stored in the Database for the specified Test Name with Sample Number and the Graph in the Graph screen will be updated automatically. If the Logging Function is OFF, the Data Points will not be stored and only the single current reading will be displayed.

41 Page: 40 6 Graph Screen Test Name Graph Oxygen Reading 4. Temperature 5. X-Axis Scale Fig 2 Graph-1: Graph Screen The specified Test Name with Sample Number in the Logging screen will be displayed on the top of the screen. Display the graph of the data points for the specified Test Name and Sample Number. If the Logging LED is green, the new data points will be displayed. If the Logging LED is red, only the current reading will be displayed. Displays the most current Oxygen concentration measured in the units chosen in the View window. 4. Displays the Temperature as per the selection in the View window. 5. X-Axis Scale a. If the button Days in the X-Axis Scale is checked, the Graph will be displayed in Days. b. If the button Hours in the X-Axis Scale is checked, the Graph will be displayed in Hours. c. If the button Minutes in the X-Axis Scale is checked, the Graph will be displayed in Minutes. d. If the button Seconds in the X-Axis Scale is checked, the Graph will be displayed in Seconds.

42 Page: 41 7 Calibration Screen Calibration da/db Self Calibration 3 Calculated da / db Fig. 23: Calibration Screen These fields and displays are utilized to perform the calibration of the OxyDot. Calibration da/db - This area allows you to enter the OxyDot Calibration (da/db) to display in the Main screen and to be utilized in the Oxygen calculation. Enter the OxyDot Calibration da value in the da field and the OxyDot Calibration db value in the db field. Self-Calibration Clicking Get Low and Get High buttons will read an Oxygen measurement with a known Oxygen concentration sample to give the Ratio value. Once both values have been obtained, the new calibration values are obtained by clicking the Calculate New da/db button. A more detailed explanation of the calibration procedure can be found in Appendix B. Calculated da/db - The resulting Calibration value after obtaining the Ratio values in the Self Calibration menu. The Calibration procedure is described in Appendix B.

43 Page: 42 8 Diagnostics Screen Diagnostics Command Activate Decay Curve Fig. 24: Diagnostics Screen Diagnostics Command: Clicking the Diagnostics Command button will open the following window so you can change the internal settings of the Gen III device within the firmware. We strongly recommend that you do not adjust these settings without direct communication with OxySense Support. The defaults have been carefully chosen to provide optimal results. Fig 25 Diagnostic Commands

44 Page: 43 Over Sample You can set the current over sampling (averaging) for each measurement by entering the value in the Over Sample field and clicking the Send button LED Pulse You can set LED pulse width by entering the value in the LED Pulse field and clicking the Send button. TF You can set the first sample moment delay by entering the value in the TF field and clicking the Send button. TS You can set second sample moment delay by entering the value in the TS field and clicking the Send button. Delay You can set the delay between LED pulses by entering the value in the Delay field and clicking the Send button. Rate You can set the time between two consecutive measurements in milliseconds by entering the value in the Rate field and clicking the Send button. A value of zero means that no automatic conversions are done. Set New Defaults If you want to set the above value as the new defaults in the Gen III Device, click the Set New Defaults button. Clicking the Set New Defaults button will display the following message: Do you want to set the value as Default in Gen III Device?. If you click the Yes button from the message box, the following window will be displayed to enter the Password before setting the Default values. If you click the No button from the message box, it will cancel the Set New Default operation. Close If you want to close the Diagnostic Command window click the Close button.

45 Page: 44 Password OK Cancel Fig. 26: Diagnostics Password Screen You must enter the Password in the Password field. OxySense Support to obtain the Password. You must contact The Password field cannot be blank. Clicking the OK button will check the password. If the entered password is incorrect, an error message will be displayed. If the Password entered is correct, the Set New Default Password window will be closed and the values will become the new default values. Clicking the Cancel button will close the Set New Default Password window without updating the default values. Activate Decay Curve: Clicking the Activate Decay Curve button will take 50 readings and display the fluorescence decay curve for the OxyDot: Fig. 27: Diagnostics Fluorescence Decay Curve

46 Page: 45 Diagnostics Screen Data a. Displays the Oxygen concentration measured in the units chosen in the View window. b. Displays the Temperature as defined in the View window. c. Displays Pressure as defined in the View window. d. Displays the Ratio. e. Display the fluorescence decay curve, when the Activate Decay Curve button is clicked.

47 Page: 46 9 Error Log At the time of executing the application, if an error occurs, the error will be stored in the database and a message box will be displayed with the error code: Do you want to Export the Error Log to OxySense? with Yes and No buttons. If you click the Yes button, the error stored in the database with the current error will be stored at the following folder path: GENIII\ErrorReport. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the above log files will be created at: C:\GENIII\ErrorReport. If there is a problem storing the error in the database, an error message will be displayed: Could not store the Error details in the Database. Do you want to Export the Error Log to OxySense?. If there is a problem retrieving the error from the database, an error message will be displayed: Could not retrieve the Error Log from the Database. Do you want to Export the Error Log to OxySense?. The file name will be ErrorLog plus current date in (ddmmyy format) for example ErrorLog_ CSV. Please contact OxySense Technical Service if the error persists. Be prepared to send the error log file to OxySense for further analysis.

48 Page: 47 4 Making Measurements Before making any measurements using the OxySense non-invasive oxygen measurement systems, the following preparation instructions must be read and followed. The OxySense instruments are designed for operation in a consistently illuminated environment. Testing should be conducted in the same light level environment for all readings. Otherwise, small variations in measurements (on the order of 0.2%) may be noted. 4.1 Preparing the Package or Container to be Measured Oxygen measurements within packages/bottles are made by first attaching an oxygensensitive sensor to the interior wall prior to filling and sealing. These sensors, called OxyDots, are shipped with the instrument and should be stored in a dark environment. The OxySense system analyses the interactions between the OxyDot and fibre pen to determine the oxygen concentration inside the package. The fluorescence can be measured through the transparent/semi-transparent package wall, the adhesive, and the substrate of the OxyDot. Some properties to keep in mind are: The OxyDot can be positioned as desired in liquid or headspace areas; Measurements can be repeated multiple times to obtain oxygen concentration in the same package; and The OxyDot must be glued in before filling and sealing the package. Use the following instructions to glue an OxyDot inside the package to be measured. Dispense a small amount of the supplied General Electric RTV 118 (or other suitable adhesive) into a disposable container. Using a glue applicator pick up a very small amount of the adhesive on the end and gently apply to the inside of the bottle where you wish to glue the OxyDot. Ideally this will be an easily accessible area for the reader pen assembly to reach on the outside of the container. The adhesive should be spread relatively thickly onto the inside surface in a small diameter, roughly equal to that of the OxyDot.

49 Page: 48 Pick up the OxyDot using the supplied vacuum pen with the coating side in contact with the pen. Press the black bar on the vacuum pen in and hold. Place the rubber tip onto the OxyDot coating side and release the bar to create a vacuum. Tip: Check both sides of the OxyDot to detect which side is reflective and shiny. This is the side without coating. Use the vacuum pen on the other side. When lifted, the OxyDot should remain on the tip of the pen. When transferring to the package, the pen should be inverted so that gravity keeps the OxyDot on the tip of the pen in case the vacuum is lost. 4. Gently press the OxyDot to the adhesive on the inside of the container. Be sure to move swiftly before the adhesive begins to cure. It is recommended that General Electric RTV 118 be used because of its low viscosity, transparency, short cure time, and high oxygen permeability. The silicone rubber will dry in approximately 90 minutes. Fig. 4.1 The OxyDot is glued to the inside wall of a transparent package with silicone rubber RTV 118 and the aid of the vacuum pen. The coating is now facing the inside of the package and sensor readings can be made from the other side of the wall. The light path is shown as a blue (excitation) and red (fluorescence) arrow. 4.2 Preparing the System for Use Once the OxyDot has been glued into the package, the system must be set up to capture oxygen readings. Follow these directions closely to ensure accuracy of the system. Turn the OxySense system on and start the OxySense Gen III software. Perform the background correction as described in section

50 Page: 49 Sample temperature is a very important element in the accuracy of the system and therefore is included in the measurements when made. The OxySense System automatically records the temperature when a capture is made. Manually entering the temperature is not required unless an application requires one to do so. 4. Click on the calibration tab, and in the fields marked da and db, enter the factory-provided calibration numbers for the OxyDot you are analyzing (return to the Main tab). 5. Click on the view button, select the unit format (Percentage, PPB or mbar) in which you would like to have the oxygen concentration reported and return to the main screen. 4.3 Taking a Reading Place the reader pen directly over (almost touching the package) the OxyDot from the outside of the transparent package wall. Illuminate the centre point of the film and hold steady. For ease of use, use a clamp and ring stand to hold the fibre pen and take the package to it for alignment. Figure 4.2 gives an idea of a typical setup. OXYDOT (inside the sample) Cables to OxySense instrument Sample of Product or Package Reader-Pen Fig. 4.2 Making a measurement with the OxySense Reader Pen. Once the pen is aligned with dot, take an oxygen measurement by pressing the button the pen or selecting the Capture soft key on the screen.

51 Page: 50 5 Permeation Measurements If the Permeation feature is enabled from the Configuration window, the Gen III-P logo will be displayed on the Top with the Film and Package Permeation Tab, if Unlocked is selected for Permeation within the Configuration Window. If the Permeation feature is not enabled, the Gen III logo will be displayed on the Top and the Permeation features (Film and Package) will not be enabled. 5.1 Film Permeation Adding a Chamber New & New Test. Adding a New Test for existing Chamber Opening an Existing Test Fig. 5.1: Film Permeation Screen 5.1 Browse Active Browse All 4. View Log File & Graph 5. Setup 6. Close Test 7. Print Report 8. Capture 9. Timer a. Chamber Serial b. Test Name Adding a New Chamber and New Test Name To enter the Serial Number of a new Chamber and Test Name into the system, enter the new Chamber Serial Number in the Chamber Serial Number field by scanning the Chamber Barcode or enter through the keyboard and click the Open button. Since the Serial Number of the Chamber does not exist in the database, a message box will be

52 Page: 51 displayed Chamber Serial Number not found. Do you want to add a New Chamber Serial Number: A If you click the Cancel button, the Chamber will not be added to your database. You must click the OK button and complete the process to add the first Test in order for the Chamber to be added. Clicking the OK button from the message box will allow you to add the new Chamber to your database by entering the first Test Name and Test Description into the following Add New Test screen. You must add the first Test to complete the addition of the Chamber to the database. a. Chamber Serial Number b. Test Name c. Description d. Film Thickness e. Add f. Cancel Fig. 5.2: Adding a New Chamber & New Test Name a. Chamber Serial Number: The previously entered new Chamber Serial Number will be displayed. b. Test Name: Enter the Test Name (maximum 17 characters) in the Test Name field. The Test Name must be unique in the system to create a New Test and cannot be blank. c. Description: Enter the Test Description (maximum 110 characters) in the Test Description field. The Test Description field is optional. d. Film Thickness: Enter the value for Film Thickness and select its unit of measure from the option buttons. e. Add: After entering the Test Name, Test Description and Film Thickness, click the Add button to add the New Test for the new Chamber. Upon clicking the Add button, the existing OxyDot Calibration confirmation message box will be displayed: The Current OxyDot Calibration is da=13 and db=0.0. Is the existing OxyDot Calibration correct?. If you click the Yes button from the message box, the Add New Test window will be closed and the New Test Name with the Serial Number of the new Chamber will

53 Page: 52 be opened in the Film Permeation tab with OxyDot calibration da=13 and db=0.0. If you click the No button from the message box, the following screen will be displayed: OxyDot Calibration Confirm OxyDot Calibration OK 4. Cancel Fig. 5.3: Adding OxyDot Calibration OxyDot Calibration: Enter the OxyDot Calibration da and db fields. Confirm OxyDot Calibration: Enter the same OxyDot Calibration da and db fields in the Confirm OxyDot Calibration fields. If the Calibration (da/db) and Confirm Calibration (da/db) do not match, an error message will be displayed. The Calibration Numbers will be entered into the application database for that Test and will utilized for all subsequent Tests for that Chamber until changed. OK: When you enter the Calibrations and click the OK button, the Add New Test window will be closed and the New Test Name with the Chamber Serial Number will be opened in the Film Permeation tab. 4. Cancel: Clicking the Cancel button will close the Add New Test window without adding a New Test and the previous screen will be displayed to specify a different Chamber or utilize the Browse Active or Browse All buttons. f. Cancel: If the Cancel button is clicked, the New Chamber Serial and Test will not be added to the database and will return to the Main screen. 5.2 Adding a New Test for an Existing Chamber To create a New Test for an existing Chamber, the prior Test must be closed. Please make note that once you have Ended a Test, you can no longer add additional readings to that Test. If the Open button displays an Oxygen Permeation Test Name when the Chamber Serial Number is entered, the previous Test has not been Ended. You must End the prior Test before attempting to open a New Test using the same Chamber. Enter the Unique Serial Number of the Chamber by scanning the Chamber Barcode or enter the Serial Number of the Chamber through the keyboard. Click the Open button. If there are no open Tests for the Chamber, a message box will be displayed: Do you want to add New Test for Chamber Serial Number: A ?.

54 Page: 53 If you click the Yes button, the following Add New Test window will open: a. Chamber Serial Number b. Test Name c. Description e. Film Thickness d. Add e. Cancel Fig. 5.4: Adding a New Test for existing Chamber. a. Chamber Serial Number: The entered Chamber Serial Number will be displayed. b. Test Name: Enter the Test Name (maximum 17 characters) in the Test Name field. The Test Name must be unique in the system to create a New Test and cannot be blank. c. Description: Enter the Test Description (maximum 110 characters) in the Test Description field. The Test Description field is optional. d. Film Thickness: Enter the value for Film Thickness and select its unit of measure from the option buttons. e. Add: After entering the Test Name and Test Description, click the Add button to add the New Test for the Chamber. Clicking the Add button will check whether the Test Name entered is already in the database. If the Test Name is found, an error message will be displayed: Test Name already exists. A duplicate Test Name is not allowed.. If the Test Name field is blank, the application will display an error message: Test Name cannot be Blank. Please type proper Test Name.. If the Test Name is correct, the application will display the following confirm existing OxyDot Calibration message with da and db values of the last test for this Chamber Serial Number: The Current OxyDot Calibration is da=13 and db=0.0. Is the existing OxyDot Calibration correct? with Yes and No buttons. If you click the Yes button from the message box, the Add New Test window will be closed and the New Test Name with the Serial Number of the new Chamber will be opened in the Film Permeation tab with OxyDot calibration da=13 and db=-0.0. If you click the No button from the message box, the following screen will be displayed:

55 Page: 54 OxyDot Calibration Confirm OxyDot Calibration OK 4. Cancel Fig. 5.5: Adding OxyDot Calibration OxyDot Calibration: Enter the OxyDot Calibration da and db fields. If the Calibration da field is blank, an error message will be displayed: Enter the valid value for the Calibration da. If the Calibration db field is blank, the application will display: Enter the valid value for the Calibration db error message. Confirm OxyDot Calibration: Enter the same OxyDot Calibration da and db fields in the Confirm OxyDot Calibration fields. If the Calibration da and Confirm Calibration da do not match, an error message will be displayed: The Calibration da and Confirm Calibration da do not match. If the Calibration db and confirm Calibration db do not match, the application will display the following error message: The Calibration db and Confirm Calibration db do not match. The Calibration Numbers will be entered into the application database for that Test, and will be utilized for all subsequent Tests for that Chamber until changed. OK: When you enter the Calibrations and click the OK button, the Add New Test window will be closed and the New Test Name with the Chamber Serial Number will be opened in the Film Permeation tab. 4. Cancel: Clicking the Cancel button will close the Add New Test window without adding a New Test and the previous screen will be displayed to specify a different Chamber or utilize the Browse Active or Browse All buttons. f. Cancel: If the Cancel button is clicked, the New Test will not be added to the database and will return to the Main screen.

56 Page: Opening an Existing Test a. Enter the unique Serial Number of the Chamber (by scanning the Chamber Barcode or enter the Serial Number of the Chamber through the keyboard). b. After entering the unique Serial Number of the Chamber or the Oxygen Permeation Test Name in the appropriate fields, click the Open button to open the Test. If a Test is Open for the Chamber, the Test will be Open and you will be able to utilize the various buttons to View Log File, View Log Graph, Capture a measurement Setup the test settings, Print Report or Close Test. 5.4 Browse Active Tests Click the Browse Active button to display the following window containing a list of Open (Active) Tests (Test Name, Chamber Number, Open Date & Time and Close Date & Time) for Film Permeation. Open Test Update Description Update Calibration 4. End Test 5. Close Fig. 5.6: Film Permeation Browse Active Tests Open Test: To open a Test, select the Test from the Open Tests list and click the Open Test button. Clicking the Open Test button closes the Browse Active window and displays the selected Test Name and Chamber Serial Number in the Film Permeation Tab screen. Clicking the Open Test button without previously selecting a Test will display the following error message: Please select the Test from the Active Test List to open. Update Description: Clicking the Update Description button displays the selected Test Name with Chamber Number in a new window with the Test Description in the following screen to allow you to update the Description field in the data base:

57 Page: 56 a. Test Name & Chamber b. Test Description c. Update Description d. Cancel Fig. 5.7: Film Permeation Update Description a. The selected Test Name and Chamber Number will be displayed. b. Update the Test Description in the Test Description field. c. Clicking the Update Description button updates the Test Description for the Selected Test Name and Chamber Number. After updating the database, the Update Description window will be closed. d. Clicking the Cancel button will close the Update Description window without updating the Test Description. Update Calibration: Clicking the Update Calibration button displays the selected Test Name and Chamber Number in a new window with the existing OxyDot Calibration. If the Calibration da/db is changed, the previous data points for the Selected Test Name and Chamber Number will be recalculated based upon the New Calibration da/db. a. Test Name & Chamber b. Test Description c. Update Description d. Cancel Fig. 5.8: Film Permeation Update OxyDot Calibration a. T he selected Test Name with Sample Number will be displayed. b. Enter the new OxyDot Calibrations in the da and db fields. If the Calibration da/db fields are empty, an error message will be displayed. c. Clicking the Update Calibration button will ask you for confirmation: Are you sure you want to update Calibration da and db. If Yes, the previous Data Points will be recalculated.. If you click the Yes button, the previous data points will be recalculated with the updated da and db for the selected Test Name and Chamber Number. If you click the No button, the Calibration da and db will not be updated.

58 Page: 57 d. 4. Clicking the Cancel button will close the Update Calibration window. End Test: Please make note that once you have Ended a Test, you can no longer add additional readings to that Test. To End a Test, select the Test from the Active Test List and click the End Test button. Clicking the End Test button will display a message box: Are you sure you want to End the Test: For Chamber Serial Number :? You will not be able to capture additional data points for an ended Test.. Clicking the OK button will end the selected Test Name and Chamber Number and remove the Test Name from the Open Tests list. Clicking the End Test button without previously selecting a Test will display an error message: Please select the Test from the Open Tests List to end the test Close: Click the Close button to close the Browse Active window. Browse All Tests Click the Browse All button to display the following window containing a list of Open (Active) Tests (Test Name, Chamber Number, Open Date & Time and Close Date & Time) followed by all Closed Tests for Film Permeation. Open Test Update Description Update Calibration 4. End Test 5. Delete Test 6. Close Fig. 5.9: Film Permeation Browse All Tests Open Test: To Open a Test, select the Test from the Open Tests list or Ended Tests list and click the Open Test button. Clicking the Open Test button closes the Browse All window and displays the selected Test Name and Chamber Serial Number in the Film Permeation Tab screen.

59 Page: 58 Clicking the Open Test button without previously selecting a Test will display an error message: Please select the Test and Chamber Number from the Open Tests List or the Ended Tests List to open the test.. Update Description: Clicking the Update Description button displays the selected Test Name with Chamber Number in a new window as per Fig. 5-7 with the Test Description. You are allowed to update the Test Description if the selected Test is Ended. Update Calibration: Clicking the Update Calibration button displays the selected Test Name with Chamber Number in a new window as per figure Fig. 5-8 with the existing OxyDot Calibration. If the Calibration da/db is changed, the previous data points for the Selected Test will be recalculated based upon the new Calibration da/db. The Calibration for an Ended Test cannot be updated. 4. End Test: To End a Test, select the Test from the Open Test List and click the End Test button. Clicking the End Test button will display a message box Are you sure you want to End the Test: For Chamber Serial Number:? You will not be able to capture additional data points for an Ended Test.. Clicking the OK button will End the selected Test and remove that specific Test from the Open Tests list and display the Test and Chamber Serial Number in the Ended Tests list. Clicking the End Test button without previously selecting a Test from the Active Test List will display an error message: Please select the Test from the Open Tests List to end the test.. 5. Delete Test: To delete a Test, select the Test from the Ended Test List and click the Delete Test button. Clicking the Delete Test button will display a message box with: Are you sure you want to Delete the Test: For Chamber Serial Number:?. Clicking the OK button will Delete the selected Test and remove that specific Test from the Closed Tests list. Clicking the Delete Test button without previously selecting a Test from the Ended Test List will display an error message: Please select the Test from the Ended Test List to delete the test Close: Click the Close button to close the Browse All window. View Log File and Log Graph To open the Film Permeation Log or Graph screens, identify a specific Chamber Serial Number based upon the options noted above. If the View Log File or View Log Graph button is clicked without entering anything in the Chamber Serial Number field, the application will display an error message: Chamber Serial Number cannot be blank.

60 Page: 59 If the View Log File or the View Log Graph button is clicked after entering a Chamber Serial that is not in the database, the application will display an error message: Could not find the entered Chamber Serial Number. Clicking the View Log File button will display the following Film Permeation Log screen with the list of Data Points, OTR, Starting and Final Data Points: Clicking the View Log Graph button will display the Film Permeation Graph screen (Fig. 5.11) with the Graph of Data Points, OTR, Starting and Final Data Points: Fig. 5.10: Film Permeation View Log. OTR Test Name Chamber 4. Starting Data Point 5. Final Data Point 6. Calibration 7. Log 8. Delete Data Point 9. Select Log 10. View Options 1 Timer Options OTR: Displays the OTR Reading for the Starting and Final Data Points. If the OTR reading is not in the range specified, a message box will be displayed: OTR should be greater than and less than , with the OTR reading in red. Test Name: Displays the selected Test Name. Chamber Number: Displays the selected Chamber Number. 4. Starting Data Point: Displays the selected starting data point (Date, Time and Oxygen (mbar) for the OTR calculation. If the Oxygen (mbar) reading is not in the range specified, a message box will be displayed: Oxygen Reading (mbar) should be greater than 0 and less than 300, with the Oxygen (mbar) reading in red. Clicking the Reset button will blank the starting data points (Date, Time and Oxygen (mbar) and the OTR value will be Select the new starting data point by clicking an entry in the Log or the Graph. 5. Final Data Point: Displays the selected final data point (Date, Time and Oxygen (mbar) for the OTR calculation. If the Oxygen (mbar) reading is not in the range specified, a message box will be displayed: Oxygen Reading (mbar) should be greater than 0 and less than 300, with Oxygen (mbar) reading in red.

61 Page: 60 Clicking the Reset button will blank the final data point (Date, Time and Oxygen (mbar) and the OTR value will be Select the new final data point by clicking an entry in the Log or the Graph. 6. Calibration: Displays the Calibration da/db for the selected Test and Chamber Number. 7. Log: Displays the Data Points for the selected Test and Chamber Number. If the View By is selected in PPB or Percent, the user can select the Data Point in PPB or Percent. However, selecting the Data Point in PPB or Percent will actually utilize the associated mbar value for the same Data Point and enter within the empty Starting or Final Data Point. 8. Delete Data Point: To delete a Data Point from the Log List, select the Data Point from the Log List and click the Delete Data Point button. Clicking the Delete Data Point button displays the following message: Are you sure you want to Delete the Selected Data Point. Clicking the OK button deletes the selected data point and removes from the Log List and Log Graph. If a data point has not been selected prior to the click of the Delete Data Point button, an error message box will be displayed: Select the Data Point from the Log List. 9. Select Log: To select another Test, click the Select Log button. Clicking the Select Log button displays the Film Permeation screen Fig View Options: View Options are categorized as Log and Graph. Each of these categories has 3 options: Oxygen, OTR and Perm. Rate (Permeation Rate). Log: a. Oxygen: This option will display a log list of Oxygen values captured with their date and time (as shown in Fig. 5.10). b. OTR: This option will display a log list of OTR values measured during the Oxygen capture with their date and time. c. Perm. Rate: This option will display a log list of Permeation Rate values with their date and time. Graph: a. Oxygen: This option will display a graph of Oxygen values versus Time. (as shown in Fig. 5.11). b. OTR: This option will display a graph of OTR values measured during the Oxygen capture versus Time. c. Perm. Rate: This option will display a graph of Permeation Rate values versus Time.

62 Page: 61 View Log Options X-Axis Scale Fig. 5.11: Film Permeation View Graph. View Log Options: Selecting any of these options will display the respective Log List. X-Axis Scale a. If the Radio button Days in the X-Axis Scale is checked, the Graph will be displayed in Days. b. If the Radio button Hours in the X-Axis Scale is checked, the Graph will be displayed in Hours. c. If the Radio button Minutes in the X-Axis Scale is checked, the Graph will be displayed in Minutes. d. If the Radio button Seconds in the X-Axis Scale is checked, the Graph will be displayed in Seconds. 1 Timer Options: Timer options will have Timer Interval and Timer Interval Unit to be specified. The Timer Interval Unit can be specified either in Seconds or in Minutes. Clicking the Timer button will automatically capture Oxygen at the specified interval of time. 5.7 Setup (Setting) To open the Setup window, identify a specific Chamber Serial Number based upon the options noted above. If the Setup button is clicked without entering anything in the Chamber Serial Number field, the application will display an error message: Chamber Serial Number cannot be blank.

63 Page: 62 If the Setup button is clicked and the Chamber Serial entered is not in the database, the application will display an error message: Could not find the entered Chamber Serial Number.. After identifying a specific Chamber Serial Number, click the Setup button to open the following Setup window: Password Change Password Cancel 4. OK Fig. 5.12: Film Permeation Setup Password Screen. Password: Enter the Password in the Password field. The factory set password is oxysense. Change Password: To change the Password, click the Change Password link to display the following screen: a. Old Password b. New Password c. Confirm Password d. OK e. Cancel Fig. 5.13: Film Permeation Change Password Screen. a. Old Password: Enter the old Password. b. New Password: Enter the new Password. c. Confirm Password: Enter the same new Password in the Confirm Password field. d. OK: Clicking the OK button will check the Old Password matches the database entry and the New Password and Confirm Password are identical. If

64 Page: 63 the Old Password matches and the New Password and Confirm Password are identical, a message box will display: Password updated. If the Old Password does not match the database entry, an error message will be displayed. If the New Password and Confirm Password are not identical, an error message will be displayed. e. Cancel: Clicking the Cancel button will display the Setup Password screen (Fig. 5.12). Cancel: Clicking the Cancel button closes the Setup Window and displays the Film Permeation Tab screen. 4. OK: After entering the correct Password and clicking the OK button, the following Permeation setting screen will be displayed. The Setup screen contains Pressure, Volume, Area, View By and View OTR By for the Chamber Serial Number and Test Name opened in the Film Permeation screen. Default Pressure Instrument Pressure Pressure (mbar) 4. Film Thickness 5. View By 6. View OTR By 7. OK 8. Cancel Fig. 5.14: Film Permeation Setup Screen. Default Pressure: If Always Use Default Pressure check box is selected, the calculation of Oxygen will use the Default Pressure value. Instrument Pressure: If Always Use Instrument Pressure check box is selected, the calculation of Oxygen will use the real time Pressure value from the Gen III device and will not use the Default Pressure value.

65 Page: 64 Default Pressure (mbar): Enter the Default Pressure value in the Default Pressure (mbar) field. If the Test is Ended, changing the Default Pressure value will display an error message: Default Pressure value cannot be changed. Test is ended.. If the Test is active and you enter a new default Pressure value in the Default Pressure (mbar) field and the value is not within the expected range, an error message will be displayed specifying the minimum and maximum range for the Default Pressure. 4. Film Thickness: Enter the film thickness value and select its unit of measurement from the options buttons. 5. View By: To change the View By, select Percent, ppb or mbar. 6. View OTR By: To change the View OTR By, select ml/m2/day or ml/100in2/day. 7. OK: If the Test is active and you select the Always Use Default Pressure check box and enter a new Default Pressure and click the OK button, the following message will be displayed: Are you sure you wish to change the Default Pressure Value? If Yes, the Oxygen reading and OTR will be recalculated for all the Samples in the Test. If you click the Cancel button, the value will not be updated. If Thickness value is changed and you click the OK button, the Setup window will be closed and the Permeation Rate values will be recalculated. If the View By is changed and you click the OK button, the Setup window will be closed and the Graph and the Log List Data Points will be displayed in the new selection in the Film Permeation Log or Graph screen. If the View OTR By is changed and you click the OK button, the Setup window will be closed and the OTR value will be calculated and displayed per the new selection in the Film Permeation Log or Graph screen Cancel: Clicking the Cancel button will close the Setup window. Timer Clicking the Timer button will start automatic capture for the selected test. automatic capture will be performed after the specified interval of time. The If the interval value is not specified and the Timer button is clicked, it will give a message to enter a valid value for Timer Interval. If any invalid data point is captured during automatic capture, the error message will have two buttons Yes and No and it will ask to stop the timer or not. Clicking the Yes

66 Page: 65 button will stop the timer and clicking the No button will continue the automatic capture. When any tab is selected other than Film Permeation, the Timer will be stopped automatically. 5.9 End Test Please make note that once you have Ended a Test, you can no longer add additional readings to that Test. To End a Test, identify a specific Chamber Serial Number based upon the options noted above and click the End Test button. If the End button is clicked without entering anything in the Chamber Serial Number field, the application will display an error message: Chamber Serial Number cannot be blank. If the End button is clicked and the Chamber Serial entered is not in the database, the application will display an error message: Could not find the entered Chamber Serial Number. If the selected Chamber Serial Number and Test Name is active, clicking the End Test will display a message box: Are you sure you want to End the Test : For Chamber Serial Number :? You will not be able to capture additional data points for an ended Test.. If the Yes button is clicked within the message box, the Chamber Serial Number and the Test will be Ended. If the selected Test Name is already Ended, clicking the End Test button will display an error message: Test is already ended Print Report To Print the Permeation Report, identify a specific Chamber Serial Number based upon the options noted above. If the Close button is clicked without entering the Chamber Serial Number field, the application will display an error message Chamber Serial Number cannot be blank. If the Close button is clicked after entering a Chamber Serial that is not in the database, the application will display an error message: Could not find the entered Chamber Serial Number.. If the selected Test is Active, clicking the Print Report button displays the following message box:

67 Page: 66 Print Interim Report Cancel Fig. 5.15: Film Permeation Print Report Message. Print Interim Report: Clicking the Print Interim Report button opens the following Print Report window. The Print Report window for an Interim Report does not contain the Close Date because the Test is active. If the Test is closed the above message will not be displayed and the Print Report window will be opened and it will contain the Close Date. Print Preview Print PDF Report 4. Close Fig. 5.16: Film Permeation Print Report. Print Preview: To view how the report will be printed, click the Print Preview button. Clicking the Print Preview button will open the Print Preview window to display how the report will be printed.

68 Page: 67 Print: To Print the Report, click the Print button. Clicking the Print button will send the report directly to the printer. PDF Report: To create a PDF document of the Report, click the PDF Report button. Clicking the PDF Report button will send the report to the PDF writer software for generating the PDF report. The PDF Report button will generate the PDF report with the selected Test Name as the File Name with Date and Time and save in a C:\GENIII\PermeationReport\Film folder and display a message box with the file saved path. 4. Close: Clicking the Close button will close the Print Report window. Print Report window Data a. Displays the selected Chamber Number. b. Test Open Date. c. Test Close Date. If the Test is Active the Close Date will be blank. d. Displays the Selected Test Name. e. Displays the selected Test Name Description. f. Displays the OTR Value in both of its units and also the Permeation Rate values in both of its units. g. Displays the Starting and Final Data Points and the Duration used for the OTR Calculation in Seconds, Minutes, Hours or Days as per the selection of the X-Axis Scale in the Film Permeation screen. h. Displays the Permeation Setting (Atmospheric Pressure, Volume, Area and Film Thickness) for the selected Test. i. Displays the Data Points for the Selected Test in the Log. j. Displays the Data Points for the Selected Test in a Graph in Seconds, Minutes, Hours or Days as per the selection of the X-Axis Scale in the Film Permeation screen After the list and graph of Oxygen data points, the report displays the list and graph of OTR as well as Permeation Rate. Cancel: Clicking the Cancel button will not open the Print Report window and cancel the printing Capture To Capture an Oxygen reading, identify a specific Chamber Serial Number based upon the options noted above.

69 Page: 68 If the Capture button is clicked without entering anything in the Chamber Serial Number field, the application will display an error message: Chamber Serial Number cannot be blank. If the Capture button is clicked after entering a Chamber Serial that is not in the database, the application will display an error message: Could not find the entered Chamber Serial Number. If the selected Chamber Serial Number and Test is active, clicking the Capture button will capture a new Oxygen reading, update the Log, update the Graph and save the Data Point in the database. If the selected Chamber Serial Number and Test is closed, clicking the Capture button will display an error message: Could not take Oxygen Measurement for Test Name. Test is Ended..

70 Page: Package Permeation Open Test New Test Browse Active 4. Browse All 5. View Log File & Log Graph 6. Setup 7. Close Test 8. Print Report 9. Capture 10. a. Timer Test Name Fig. 5.17: Package Permeation Screen 5.1 Open Test Enter the Test Name with Sample Number in the Permeation Test Name field by reading a Barcode or entering through the Keyboard. If the entered Test Name with Sample Number in the Test Name field is correct, the Open button will be enabled else it will be disabled. If the entered Test Name with Sample Number in the Test Name field is incorrect, the Open button will be disabled and the error message: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number. will be displayed. 5.2 Adding a New Test To add a new Test with Samples, click the New Test button. Clicking the New Test button will display the following Add New Test window:

71 Page: 70 Fig.5.18: Package Permeation Add New Test Screen. Test Name Description Number of Samples 4. Unique Sample Description 5. Volume 6. Create All Bar codes 7. Add 8. Cancel Test Name: Enter the Test Name (maximum 17 characters) in the Test Name field. The Test Name must be unique in the system to create a New Test and cannot be blank. Test Description: Enter the Test Description (maximum 110 characters) in the Test Description field. The Test Description field is optional. Number of Samples: Enter the Number of Samples in the Number for Samples field. The Number of Samples field cannot be blank and cannot be greater than Unique Sample Description: If the Test will have Unique Sample Descriptions, select Yes from the Unique Sample Descriptions combination drop down box. If No is selected, the Sample Description will be blank for all of the Samples. 5. Volume: Enter the Volume in the Volume field. The Volume field cannot be blank and should be in the range of 0 to Create All Bar Codes: After entering the required information (Test Name, Description, Number for Sample and Unique Sample Description), to create all of the Barcodes for the entered Test Name and number of Samples, click the Create All Bar Codes button. Clicking the Create All Bar Codes button will open the following Barcode window if the Number of Samples = 1 or the Unique Sample Descriptions = No. If the Number of Samples is > 1 and the Unique Sample Descriptions = Yes, the following message will be displayed: Are you sure you want to print all of the Bar Codes prior to entering the Unique Sample Descriptions?. If the Yes button is clicked, the Barcode window will be displayed. If the No button is clicked, the Unique Sample Description screen to enter the Unique Sample Descriptions for all of the Samples will be displayed.

72 Page: 71 Width Height Top Margin 4. Left Margin 5. Orientation 6. Print Preview 7. Print 8. Close Fig.5.19: Package Permeation Barcode Screen If you have not changed the Barcode labels from the labels delivered, the defaults are pre-filled and you may go directly to Print or Print Preview. Width: Enter the width in the Width field in inches for printing specific Barcode Label width. Height: Enter the height in the Height field in inches for printing specific Barcode Label height. Top Margin: Enter the top margin in the Top Margin field in inches for printing the Barcode Label. 4. Left Margin: Enter the left margin in the Left Margin field in inches for printing the Barcode Label. 5. Orientation: Select either the Horizontal or Vertical radio button from the Orientation. 6. Print Preview: To see how the Barcode will print, click the Print Preview button Print: To Print the Barcode, click the Print button. 8. Close: To close the Barcode window, click the Close button. Add Button Processing: After entering the Test Name, Volume, Test Description, Number of Samples and Unique Sample Descriptions (Yes/No) click the Add button to add the New Test Name with Number of Samples to the database. Clicking the Add button will check whether the Test Name entered is already in the database. If the Test Name is found, the application will display an error message: Test Name already exists. A duplicate Test Name is not allowed. If the Test Name field is blank, the application will display an error message: Test Name cannot be Blank. Please type proper Test Name.

73 Page: 72 If the Test Name entered is correct, the application will check the Number of Samples. If the Number of Sample field is 0, the application will display an error message: Number of Samples should be greater than Please type proper Sample Number.. If the Test Name and Sample Number are correct, the application will check the Volume. If the Volume field value is not in the specified range, an error message will be displayed: Incorrect Volume, enter Volume between 0 and Please type proper Volume. If the Test Name, Sample Number and Volume are correct, the application will display the following confirm existing OxyDot Calibration message: The Current OxyDot Calibration is da=13 and db=0.0. Is the existing OxyDot Calibration correct? with Yes and No buttons. a. Yes : If you click the Yes button and if Yes is selected from the Unique Sample Descriptions combination drop down box, the Add New Test will be changed to Fig to enter the Sample Description. If No is selected from the Unique Sample Descriptions combination drop down box, the Add New Test window will be closed and the New Test Name with the Sample Number will be opened in the Package Permeation tab with OxyDot calibration da=13 and db=0.0. b. No : If you click the No button, the Add New Test window will be changed to the following screen to allow you to add unique OxyDot Calibrations for each of the Samples: OxyDot Calibration Confirm OxyDot Calibration Sample Number 4. OK 5. Cancel Fig.5.20: Package Permeation OxyDot Calibration Screen OxyDot Calibration: Enter the OxyDot Calibration da and db fields in the OxyDot Calibration fields. If the Calibration da field is blank, the following error message will be displayed: Enter the valid value for the Calibration da.

74 Page: 73 If the Calibration db field is blank, the following error message will be displayed: Enter the valid value for the Calibration db. Confirm OxyDot Calibration: Enter the same OxyDot Calibration da and db fields in the Confirm OxyDot Calibration fields. If the Calibration da and confirm Calibration da do not match, the following error message will be displayed: The Calibration da and Confirm Calibration da do not match. If the Calibration db and Confirm Calibration db do not match, the following error message will be displayed: The Calibration db and Confirm Calibration db do not match. Sample Number: Allows you to specify a range of Samples to apply the OxyDot Calibration (da/db) entered. If the Sample Number is not in the range, an error message will be displayed. 4. OK: Clicking the OK button will add the Calibration (da/db) for the selected Samples in the database. When the Calibration (da/db) for all of the Samples have been entered, the OxyDot Calibration window will be changed to the following screen to allow you to add unique Sample Descriptions if the Unique Sample Descriptions field = Yes. If the Unique Sample Descriptions = No, the Add New Test Window will be closed and the Test Name with Sample 1 will be open in the Package Permeation screen. a. Sample Description b. Create Test Sample Barcode c. Next d. Close Fig.5.21: Package Permeation Add Sample Description Screen a. Sample Description: The Sample Description box will allow you to sequentially enter the unique Sample Description for each Sample Number. Enter the description in the Sample Description field and click the Next button to go to the Next Sample Number.

75 Page: 74 When the unique Sample Descriptions have been entered for all of the Samples, the message box: Do you want to print the Bar Codes for all of the Samples? will be displayed. If the Yes button is clicked, the Barcode screen (Fig. 5.19) will be displayed. The Print button will print all of the Barcodes for all of the Samples. Clicking the No button will close the Add Sample window and return to the Package Permeation Tab screen with the Test Name and Sample 1 displayed. b. Create Test Sample Barcode: To Create the Barcode for the specific Sample, click the Create Test Sample Barcode button to print the Barcode for each Sample one at a time while entering the unique Sample Description. c. Next: Clicking the Next button will display the Next Sample Number to enter the unique Sample Description. d. Close: clicking the Close button will close the Add New Test window and the Test Name with Sample 1 will be open in the Logging screen Cancel: Clicking the Cancel button will display the (Fig. 5.18) screen. Cancel: Clicking the Cancel button will close the Add New Test window. Browse Active Clicking the Browse Active button displays the following window. The window contains a list of the Open (Active) Tests (Test Name and Open Date & Time) for Package Permeation. Open Test Update Volume Update Description 4. Update Calibration Fig.5.22: Package Permeation Browse Active Test 5. End Test 6. Print Barcode 7. Close Open Test: To Open a Test, select the Test from the Open Tests list and click the Open Test button. Clicking the Open Test button closes the Browse Active

76 Page: 75 window and displays the selected Test Name with Sample number in the Package Permeation Tab screen. Clicking the Open Test button without previously selecting a Test will display a message box with: Please select the Test from the Open Test List to open the test.. Update Volume: Clicking the Update Volume button displays the selected Test in a new window with Test Name and Volume in the following screen to allow you to update the Volume for all Samples within the Test. If you clicks the Update button, the application will display a message: Are you sure you want to update the Volume for all Samples within the Test?. If you click Yes, the Volume field in the database for all Samples of the Test will be updated and the OTR for all Samples will be recalculated. a. Test Name b. Volume c. Update Volume d. Cancel Fig.5.23: Package Permeation Update Volume a. Selected Test Name with Sample Number will be displayed. b. Update the Volume in the Volume field. The Volume field cannot be blank and should be in the range of 0 to c. Clicking the Update Volume button the Volume for the selected Test Name and all the Sample Numbers will be updated. After updating, the Update Volume window will be closed. d. Clicking the Cancel will close the Update Volume window. Update Description: Clicking the Update Description button displays the selected Test Name with Sample Number in the following screen to allow you to update the Description and Sample Description field in the data base:

77 Page: 76 a. Test Name b. Test Description c. Sample Description d. Update Description Fig.5.24: Package Permeation Update Description e. Cancel a. Selected Test Name with Sample Number will be displayed. b. Update the Test Description in the Test Description field. c. Update the Sample Description in the Sample Description field. d. Clicking the Update Description button the Test Description and Sample Description for the Selected Test Name and Sample Number will be updated. After updating, the Update Description window will be closed. e. 4. Clicking the Cancel button the Update Description window will be closed. Update Calibration: Clicking the Update Calibration button displays the selected Test in the following screen to allow you to update the Calibration. If the Calibration da/db is changed, the previous data points for the Selected Test and the selected Samples will be recalculated as per the New Calibration da/db. a. Test Name b. OxyDot Calibration c. Sample Number d. Update Calibration Fig.5.25: Package Permeation Update Calibration (da/db) e. Cancel a. Selected Test Name with Sample Number will be displayed. b. Enter the New OxyDot Calibration da in Calibration da field and New OxyDot Calibration db in Calibration db field. If the Calibration da/db field is empty, an error message will be displayed.

78 Page: 77 c. For storing the entered New OxyDot Calibration (da/db) for selected Samples enter the Starting Sample Number in From field and Ending Sample Number in End field. If the Sample Number is not in the range, an error message will be displayed. d. Clicking the Update Calibration button will ask you for confirmation: Are you sure you want to update the Calibration da and db? If Yes, the previous Data Points will be recalculated for the selected Samples.. If you click the Yes button from the message box, the previous data points will be recalculated with the updated da and db for the Samples Numbers entered in From field to To field. If you click the No button, the Calibration da and db will not be updated. e. 5. Clicking the Cancel button will close the Update Calibration window. End Test: Please make note that once you have Ended a Test, you can no longer add additional readings to that Test. To End a Test, select the Test from the Active Test List and click the End Test button. Clicking the End Test button will display a message box: Are you sure you want to End the Test? You will not be able to capture additional data points for an ended Test.. Clicking the OK button will display another message: End All Samples within the Test? For the Test. Clicking the Yes button from the second message box will End all Samples within the selected Test Name and remove the selected Test and all the Samples from the Open Tests list. Clicking the No button will only End the selected Test Name and Sample Number and remove only that specific Test and Sample from the Open Tests list. Clicking the End Test button without previously selecting a Test will display an error message: Please select the Test from the Open Tests List to end the test. 6. Print Barcode: Clicking the Print Barcode button will determine if the selected Test Name has multiple samples. If the test has more than one sample, a message box will be displayed: Do you want to reprint the Barcode for the Test :? with All Samples, Selected Sample and No buttons. If you click the All Samples button, the Barcode window will be opened to allow you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a new window and display a print preview window with the Test Name and the last Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and all of the Sample Numbers.

79 Page: 78 Close: Clicking the Close button will close the Barcode window. If you click the Selected Samples button, the Barcode window will be opened to allow you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a new window and display a print preview window with the Test Name and selected Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and selected Sample Number. Close: Clicking close button will close the Barcode window. If you click the No button, the Print Barcode message box will be closed. If the test has one sample, a message box will be displayed: Do you want to reprint the Barcode for the Test :? with Yes and No buttons. If you click the Yes button, the Barcode window will be opened to allow the you to set the margin, height, width and orientation. The Barcode window will contain three buttons: Print Preview, Print and Close. Print Preview: Clicking the Print Preview button will open a print preview window with the Test Name and Sample Number. Print: Clicking the Print button will print the Barcode for the selected Test and Sample Number. Close: Clicking the Close button will close the Barcode window. If you click the No button, the Print Barcode message box will be closed Close: To close the Browse Active window, click the Close button. Browse All Clicking the Browse All button displays the following window containing a list of Open (Active) Tests (Test Name and Open Date & Time) followed by all Closed Tests (Test Name, Open Date & Time and Close Date & Time) for Package Permeation.

80 Page: 79 Open Test Update Volume Update Description 4. Update Calibration 5. End Test 6. Delete Test 7. Close Fig.5.26: Package Permeation Browse All Tests Open Test: To Open a Test, select the Test from the Open Tests list or Closed Tests list and click the Open Test button. Clicking the Open Test button closes the Browse All window and displays the selected Test Name with Sample Number in the Package Permeation screen. Clicking the Open Test button without previously selecting a Test will display an error message: Please select the Test and Sample Number from the Open Tests List or the Ended Tests List to open the test.. Update Volume: Clicking the Update Volume button displays the selected Test in a new window as per Fig with Test Name and Volume in the following screen to allow you to update the Volume for all Samples within the Test. If you click the Update button, the application will display a message: Are you sure you want to update the Volume for all Samples within the Test?. If you click the Yes button, the Volume field in the database for all Samples of the Test will be updated and the OTR for all Samples will be recalculated. The Volume for the Ended Test List cannot be updated. Update Description: Clicking the Update Description button displays the selected Test Name with Sample Number in a new window as per Fig with the Test Description and Sample Description in the following screen to allow you to update the Description. You are allowed to update the Test Description and Sample Description if the selected Test is Ended. 4. Update Calibration: Clicking the Update Calibration button displays the selected Test in a new window as per Fig with Test Name, OxyDot Calibration and the Sample Number in the following screen to allow you to update the Calibration.

81 Page: 80 However, if the Calibration da/db is changed, the previous data points for the Selected Test and the selected Samples will be recalculated utilizing the new Calibration da/db. The Calibration for the Ended Test List cannot be updated. 5. End Test: Please make note that once you have Ended a Test, you can no longer add additional readings to that Test. To End a Test, select the Test from the Active Test List and click the End Test button. Clicking the End Test button will display a message box: Are you sure you want to End the Test? You will not be able to capture additional data points for an Ended Test.. Clicking the OK button will display another message box: End All Samples within the Test? For the Test. Clicking the Yes button from the second message box will End all Samples within the selected Test Name and remove the selected Test and all the samples from the Open Tests list. Clicking the No button will only End the selected Test Name and Sample Number and remove only that specific Test and Sample from the Open Tests list. Clicking the End Test button without previously selecting a Test from the Active Test List will display an error message: Please select the Test from the Open Tests List to End the test.. 6. Delete Test: To Delete a Test, select the Test from the Closed Tests list and click the Delete Test button. Clicking the Delete Test button will display a message box: Are you sure you want to Delete the Test? Clicking the OK button will display another message box: You want to delete All Samples within the Test? For the Test. Clicking the Yes button from the second message box will delete all Samples within the selected Test Name and remove from the Closed Tests list. Clicking the No button will delete only the selected Test Name and Sample number and remove it from the Ended Tests list. Clicking the Delete Test button without previously selecting a Test from the Closed Test List will display an error message: Please select the Test from the Ended Tests List to Delete the test Close: Click the Close button to close the Browse All window. View Log File and Log Graph To open the Film Permeation Log or Graph screens, identify a specific Test based upon the options noted above. If the View Log or Log Graph button is clicked without entering anything in the Permeation Test Name field, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name..

82 Page: 81 If the View Log or Log Graph buttons are clicked after entering a Test Name with Sample Number that is not in the database, an error message will be displayed: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number.. Clicking the View Log File button will display the following Film Permeation Log screen with the list of Data Points, OTR, Starting and Final Data Points: Clicking the View Log Graph button will display the Film Permeation Graph screen (Fig. 5.28) with the Graph of Data Points, OTR, Starting and Final Data Points: OTR Test Name Starting Data Point 4. Final Data Point 5. Calibration 6. Log 7. Delete Data Point 8. Select Log 9. View Options 10. Timer Options Fig.5.27: Package Permeation View Log. OTR: Displays the OTR Reading for Starting and Final Data Points. If the OTR reading is not within the range specified, a message box will be displayed: OTR should be greater than and less than , with the OTR reading in red. Test Name: Displays the selected Test Name and Sample Number. You can enter a new Test Name with Sample Number in the Test Name field by reading a Barcode or entering through the Keyboard. If the Test Name with Sample Number is incorrect, and error message will be displayed: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number.. Starting Data Point: Displays the selected starting data point (Date, Time and Oxygen (mbar) for the OTR calculation. If the Oxygen (mbar) reading is not within the range specified, a message box will be displayed: Oxygen Reading (mbar) should be greater than 0 and less than 300, with the Oxygen (mbar) reading in red.

83 Page: 82 Clicking the Reset button will blank the starting data point (Date, Time and Oxygen (mbar) and the OTR value will be To select a new starting data point, click an entry in the Log or a data point on the Graph. 4. Final Data Point: Displays the selected final data point (Date, Time and Oxygen (mbar) for the OTR calculation. If the Oxygen (mbar) reading is not within the range specified, a message box will be displayed: Oxygen Reading (mbar) should be greater than 0 and less than 300, with the Oxygen (mbar) reading in red. Clicking the Reset button will blank the final data point (Date, Time and Oxygen (mbar) and the OTR value will be To select a new final data point, click an entry in the Log or a data point on the Graph. 5. Calibration: Displays the Calibration da/db for the selected Test and Chamber Number. 6. Log: Displays the Data Points for the selected Test and Chamber Number. If the View By is selected in PPB or Percent, the user can select the Data Point in PPB or Percent. However, selecting the Data Point in PPB or Percent will actually utilize the associated mbar value for the same Data Point and enter within the empty Starting or Final Data Point. 7. Delete Data Point: To delete a Data Point from the Log List, select the Data Point from the Log List and click the Delete Data Point button. Clicking the Delete Data Point button displays: Are you sure you want to Delete the Selected Data Point message box. Clicking the OK button deletes the selected data point and removes from the Log List and Log Graph. If a data point is not selected prior to clicking the Delete Data Point button, an error message will be displayed: Select the Data Point from the Log List. 8. Select Log: To select another Test, click the Select Log button. Clicking the Select Log button displays the Package Permeation screen Fig View Options: View Options are categorized as Log and Graph. Each of these categories has 2 options: Oxygen and OTR. Log: a. Oxygen: This option will display a log list of Oxygen values captured with their date and time (as shown in Fig. 5.27). b. OTR: This option will display a log list of OTR values measured during the Oxygen capture with their date and time. Graph: a. Oxygen: This option will display a graph of Oxygen values versus Time. (as shown in Fig. 5.28). b. OTR: This option will display a graph of OTR values measured during the Oxygen capture versus Time.

84 Page: 83 View Log Options X-Axis Scale Fig.5.28: Package Permeation View Graph. View Log Options: Selecting any of these options will display the respective Log List. X-Axis Scale a. If the Radio button Days in the X-Axis Scale is checked, the Graph will be displayed in Days. b. If the Radio button Hours in the X-Axis Scale is checked, the Graph will be displayed in Hours. c. If the Radio button Minutes in the X-Axis Scale is checked, the Graph will be displayed in Minutes. d. If the Radio button Seconds in the X-Axis Scale is checked, the Graph will be displayed in Seconds. 10. Timer Options: Timer options will have Timer Interval and Timer Interval Unit to be specified. The Timer Interval Unit can be specified either in Seconds or in Minutes. Clicking the Timer button will automatically capture Oxygen at the specified interval of time. 5.6 Setup (Setting) To open the Setup window, identify a specific Test based upon the options noted above. If the Setup button is clicked without entering anything in the Permeation Test Name field, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name.. If the Setup button is clicked after entering a Test Name with Sample Number that is not in the database, an error message will be displayed: Could not find the Permeation

85 Page: 84 Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number.. After identifying a specific Test Name and Sample Number, click the Setup button to open the following Setup window: Password Change Password Cancel 4. OK Fig.5.29: Package Permeation Setup Password Screen. Password: Enter the Password. The factory set password is oxysense. Change Password: To change the Password, click the Change Password link to display the following screen: a. Old Password b. New Password c. Confirm Password d. OK e. Cancel Fig.5.30: Package Permeation Change Password Screen. a. Old Password: Enter the old Password. b. New Password: Enter the new Password. c. Confirm Password: Enter the same new Password in the Confirm Password field. d. OK: Clicking the OK button will check the Old Password matches the database entry and the New Password and Confirm Password are identical. If the Old Password matches and the New Password and Confirm Password are identical a message box will display: Password updated.

86 Page: 85 If the Old Password does not match the database entry, an error message will be displayed. If the New Password and Confirm Password are not identical, an error message will be displayed. e. Cancel: Clicking the Cancel button displays the Setup Password screen (Fig.5.29). Cancel: Clicking the Cancel button closes the Setup Window and displays the Package Permeation screen. 4. After entering the correct Password and clicking the OK button, the following Permeation setting screen will be displayed. If the entered Password is incorrect an error message will be displayed. The Setup screen contains Pressure and View By for the Test Name opened in the Package Permeation screen. Default Pressure Instrument Pressure Pressure (mbar) 4. View By 5. OK 6. Cancel Fig.5.31: Package Permeation Setup Screen. Default Pressure: If the Always Use Default Pressure check box is selected, the calculation of Oxygen will use the Default Pressure value. Instrument Pressure: If the Always Use Instrument Pressure check box is selected, the calculation of Oxygen will use the real time Pressure value from the Gen III device and will not use the Default Pressure value. Default Pressure (mbar): Enter the Default Pressure value in the Default Pressure (mbar) field. If the Test is closed, changing Default Pressure value will display an error message: Default Pressure value cannot be changed. Test is closed. If the Test is active and you enter a new default Pressure value in the Default Pressure (mbar) field and the value is not within the expected range, an error message will be displayed specifying the minimum and maximum range for Default Pressure.

87 Page: View By: To change the View By ; select Percent, ppb or mbar. 5. OK: If the Test is active and you select the Always Use Default Pressure check box and enter a default Pressure in the appropriate field and click the OK button, the following message will be displayed: Are you sure you wish to change the Default Pressure value? If OK, the Oxygen reading and OTR will be recalculated for all the Samples in the Test. If you click the Cancel button, the values will not be updated. If you click the OK button, the value will be updated in the database for the open Test Name and Sample Number displayed in the Package Permeation screen and the Setup window will be closed. The existing Oxygen readings of the existing Data Points must be recalculated. If the View By is changed and you click the OK button, the Setup window will be closed and the Graph and the Log List Data Points will be displayed in the new selection in the Package Permeation Log or Graph screen. 6. Cancel: Clicking Cancel button will close the Setup window. 5.7 Timer Clicking the Timer button will start the automatic capture for the selected Test. Automatic capture will be performed after the specified interval of time. If the interval value is not specified and the Timer button is clicked, a message to enter a valid value for Timer Interval will be displayed. If any invalid data point is captured during automatic capture, the error message will have two buttons Yes and No and it will ask to stop the timer or not. Clicking the Yes button will stop the timer and clicking the No button will continue the automatic capture. When any tab is selected other than Package Permeation, the Timer will be stopped automatically. 5.8 End Test Please note that once you have Ended a Test, you can no longer add additional readings to that Test. To End a Test, identify a specific Test Name with Sample Number based upon the options noted above and click the End Test button. If the End button is clicked without entering anything in the Permeation Test Name field, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name.. If the End button is clicked after entering a Test Name with Sample Number that is not in the database, an error message will be displayed: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number..

88 Page: 87 If the selected Test Name with Sample Number is active, clicking the End Test button will display a message box: Are you sure you want to End the Test? You will not be able to Capture additional data points for an Ended Test. Clicking the OK button will display another message box: End All Samples within the Test? For the Test. Clicking the Yes button from the second message box will End all Samples within the selected Test. If the selected Test Name with Sample Number is already ended, clicking the End Test button will display: The Test is already ended message. 5.9 Summary Report To Print the Summary Report, identify a specific Test Name with Sample Number based upon the options noted above. If the Print Report button is clicked without entering anything in the Permeation Test Name field, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name.. If the Print Report button is clicked after entering a Test Name with Sample Number that is not in the database, an error message will be displayed: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number.. Clicking the Print Report button displays a message box if the Number of Samples for the Test > 1 with: Do you wish to print a Summary Report for all of the Samples? with Yes and No buttons. If you click the Yes button, the following window will be displayed with a Summary Report including both Open and Closed Tests and Samples.

89 Page: 88 Print Preview Print PDF Report 4. Next / Previous 5. Close Fig.5.32: Package Permeation Summary Report Print Preview: To view how the report will be printed, click the Print Preview button. Clicking the Print Preview button will open the Print Preview window to display how the report will be printed. Print: To Print the Summary Report, click the Print button. Clicking the Print button will send the summary report directly to the printer. PDF Report: To create a PDF document of the Report, click the PDF Report button. Clicking the PDF Report button will send the report to the PDF writer software for generating the PDF report. The PDF Report button will generate the PDF report with the selected Test Name as the File Name with Date and Time and save at the following folder path: GENIII\PermeationReport\Summary folder and display message box with the file saved path. These folders will be created under the local drive where the OxySense Gen III software is installed. For example, if the OxySense Gen III software is installed in C: drive, the above log files will be created at: C:\GENIII\ PermeationReport\Summary. 4. Next / Previous: If the Selected Test has more than one sample the Next >> button will be displayed. Clicking the Next >> button the next Sample will be displayed

90 Page: 89 with the Sample details and Data Points. The Next >> button will not be displayed when the last Sample is selected. If the Selected Test has more than one sample and you click on the Next >> button the << Previous button will be displayed. Clicking the <<Previous button will display the previous Sample with the Sample details and Data Points. The << Previous button will not be displayed when the first Sample is selected. 5. Close: Clicking the Close button will close the Print Report window. Summary Report window Data The summary report contains 3 Samples details per page. a. Test Name, Description, created Test and Number of Samples in the test will be displayed for the selected Test. b. Displays the Sample Number, sample Description and the created Date and Time for the selected Sample. c. Displays the OTR Value, Starting, Final Data Points and the Duration used for the OTR Calculation in Seconds, Minutes, Hours or Days as per the selection of the X-Axis Scale in the Package Permeation screen for the selected Test Name and Sample Numbers Print Report To Print the Report, identify a specific Test Name with Sample Number based upon the options noted above. If the Print Report button is clicked without entering anything in the Permeation Test Name field, an error message will be displayed: Test Name cannot be Blank. Please type proper Test Name.. If the Print Report button is clicked after entering a Test Name with Sample Number that is not in the database, an error message will be displayed: Could not find the Permeation Test Name and Sample Number. Please type the correct Permeation Test Name and Sample Number.. Clicking the Print Report button displays a message box if the Number of Samples for the Test > 1 with: Do you wish to print a Summary Report for all of the Samples? with Yes and No buttons. If you click the No button, the application will proceed to the next message box selection for Printing Sample Report with Log and Graph.

91 Page: 90 Print Interim Report Cancel Fig.5.33: Package Permeation Print Report Message. Cancel: Clicking the Cancel button will cancel the Print procedure and the Package Permeation screen will be displayed. Print Interim Report: Clicking the Print Interim Report button opens another message box as follows: Close All Samples Print Interim Report Cancel Fig.5.34: Package Permeation Print Report Message. Cancel: Clicking the Cancel button will cancel the Print procedure and the Package Permeation screen will be displayed. Print Interim Report: Clicking the Print Interim Report button from the above message box opens the Print Report window similar to (Fig. 5.36). The Print Report window does not contain the Close Date because the Test with Sample Number is active. The window will also not contain Next and Previous button because the single selected active Test and Sample Number will be displayed. Close All Samples: Clicking the Close All Samples button will close all of the Samples within the Test and open a new message box as follows: Print All Samples Print current sample only Fig.5.35: Package Permeation Print Report Message. Print Current Sample: Clicking the Print Current Sample button opens the Print Report window similar to (5.36). The Print Report window does not contain Next and Previous buttons because a single selected Test and Sample Number will be displayed.

92 Page: 91 Clicking the Print All Samples button from the above message box opens the following Print Report window. If the selected Test is closed, clicking the Print Report button displays the (Fig. 5.35) message box. Print Preview Print PFD Report 4. Next/Previous 5. Close Fig.5.36: Package Permeation Print Report. Print Preview: To view how the report will be printed, click the Print Preview button. Clicking the Print Preview button will open the Print Preview window to display how the report will be printed. Print: If a Single Report or Interim Report is selected and you wish to Print the Report, click the Print button. Clicking the Print button will send the report directly to the printer for printing. If Print All Samples is selected and you wish to print the Report, click the Print button. Clicking the Print button displays a message with: Do you want to Print All Samples?. If the Yes button is clicked, all of the Samples reports will be sent to the printer for printing. If the No button is clicked, only the selected sample report will be sent to the printer for printing. PDF Report: If the Single Report or Interim Report is selected and you wish to create a PDF document of the Report, click the PDF Report button. Clicking the PDF Report button will send the report to the PDF writer software for

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