Moli-bank: the Biobank of the Moli-sani project

Transcription

1 Moli-bank: the Biobank of the Moli-sani project M. B. Donati, A. De Curtis, L. Iacoviello Laboratori di Ricerca Fondazione di Ricerca e Cura Giovanni Paolo II, Università Cattolica, Campobasso

2 Science meets the people An observational cross-sectional and prospective epidemiologic study on genetics and lifestyle for a better health

3 Why cardiovascular disease and tumors? The common soil hypothesis

4 The Moli- sani study identikit 25, 000 people living in Molise Aged 35 years or more Recruitment phase: follow- up every 5 years Study duration 8 years

5 March 2005 April Recruited 24, 600 participants

6 Moli-sani recruitment strategy The cohort of the Moli-sani Project recruited from about 200,000 persons, aged >=35 years, resident in the Molise region. The Molise region covers an area of 4,438 Km2, including 136 towns. Participants recruited from city-hall registries by a multistage sampling. Firstly townships sampled in major areas of Molise region, starting from the main cities (Campobasso, Termoli) by cluster sampling, then, within each township, participants selected by simple random sampling, using electronically generated numbers.

7 A typical Moli-sani day The arrival Volunteers arrive fasting in the rooms of the Catholic University of Campobasso specifically arranged for the Moli-sani Project Here volunteers are welcomed by a secretary team that, after making them sign the informed consensus, provides them with a barcode

8 A typical Moli-sani day The biological sample drawing Blood, in order to analyze several parameters, but also to extract DNA as to study genetic characteristics of the participants Urine, to evaluate the levels of some substances

9 A typical Moli-sani day The clinical visit Electrocardiogram Spirometry Blood pressure Weight and height (BMI) Body fat distribution

10 A typical Moli-sani day Questionnaires Anamnestic questionnaire provides the basic situation of each volunteer from a clinical and lifestyle point of view Food frequency questionnaire provides information about one of the main environmental factors involved in cardiovascular and tumor risk

11 Follow-up 1. SDO and ReNCAM started on June 2010 (6 months delay), every 6 months update, CVD, cancer and deaths 2. Phone follow-up, July 2010,deaths, CVD, cancer, diabetes, hypertension and hypercholesterolemia

12 Who Moli-sani are Classi di Età 7% 17% 24% 25% 27% years years years years > 75 years

13 Stefania 90 years & Renato 99 years

14 A typical Moli-sani day In the Laboratory Biological samples test Cholesterol, HDL, TG Glucose, C Reactive Protein D-dimers Blood cell count Buffy coats for DNA extraction Biological samples aliquoted and stored in liquid nitrogen

15 The Moli-Bank straws Serum Plasma citrate Plasma EDTA Buffy coats (DNA) Urine

16 MOLI-SANI BIOBANK 4,083 goblets filled in 28 paillettes for each patient 8 red for plasma EDTA 6 blu for plasma citrate 8 yellow for serum 6 green for pellet

17 MOLI-SANI BIOBANK Urinary spot: 3hr-collection in the morning 4 vials, 5 ml for each subject

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19 Stock system Each tank (1) is divided in four sectors (2) identified by colours. 1 2 Each sector contains 35 tubes (canisters), able to store, one on the other, 5 goblets. Goblets are plastic cylinders with 12 prisms of different colours, where straws are positioned (3,4). 3 4

20 Stock system Each prism contains 14 straws, filled with biological materials by using a authomatic filling machine.

21 Moli-sani sample preparation

22 Bar-cod printing

23 Sample management in tanks To archive, retraive and manage a so high number of samples, we have developped a ad hoc, home made software

24 Sample management at-80 C

25 Only authorised, trained personnel can enter the biobank through FINGER PRINT IDENTIFICATION

26 Safety and control

27 Moli-bank has been inaugurated December 2006 by Luigi Luca Cavalli Sforza Moli-bank was realised in collaboration with BIO-REP e SAPIO

28 MOLI-SANI Investigators Chairman: Licia Iacoviello Steering Committee: Maria Benedetta Donati and Giovanni de Gaetano (Chairmen), Simona Giampaoli (Istituto Superiore di Sanità, Rome, Italy) Ethics and data monitoring committee: Jos Vermylen (University of Leuven, Belgium), Chairman, Ignacio De Paula Carrasco (UCSC, Rome, Italy), Enrico Garaci (Roma, Italy) Event adjudicating committee: Deodato Assanelli (Brescia, Italy), Francesco Alessandrini (Campobasso, Italy), Vincenzo Centritto (Campobasso, Italy), Paola Muti (Roma, Italy), Holger Schünemann (Hamilton, Canada), Pasquale Spagnuolo (Termoli, Italy), Dante Staniscia (Termoli, Italy), Sergio Storti (Campobasso, Italy) Bio-Banking: Amalia De Curtis, (Coordinator) Sara Magnacca, Maria Spinelli Scientific and organizing secretariat: Francesco Zito (Coordinator, Campobasso and Termoli, Italy), Americo Bonanni (Campobasso, Italy), Chiara Cerletti (Campobasso, Italy), Amalia De Curtis (Campobasso, Italy), Augusto Di Castelnuovo (Campobasso, Italy), Licia Iacoviello (Campobasso, Italy), Antonio Mascioli (Campobasso, Italy), Marco Olivieri (Campobasso, Italy).

29 Irene Di Stefano Francesca Fanelli Alessandra Molinaro Emanuela Plescia Iolanda Santimone Anna Sciaretta Isabella Sorella Agnieska Pampuch Agostino Pannichella Antonio R Vizzarri Giovanna Galluppo Dolores D'Angelo Rosanna Ramacciato Questionari Questionari Questionari Questionari Questionari Questionari Questionari Prelievi Prelievi Prelievi Call Center Call Center Call Center Americo Bonanni Francesca De Lucia Maria Laura Bonaccio Amalia De Curtis Cristian Silvestri Federico Marracino Sara Magnacca Genetica Molecolare Daniela Cugino Mirella Graziano Maria Carmela Latella Monica de Gaetano Gianni Quacquaruccio Iolanda Santimone Augusto Di Castelnuovo Simona Costanzo Romina Di Giuseppe Francesco Gianfagna Marco G. Olivieri Maurizio Giacci Dario Petraroia Antonella Padulo Branislav Vohnout Responsabile Comunicazione Responsabile Biobanca Responsabile Statistica Responsabile Informatica Coordinatore Studio Moli-Family