ENP SPRING SCHOOL 2013

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1 with support ENP SPRING SCHOOL 2013 Optical Imaging and Electrophysiological Recording in Neuroscience Tuesday, June 18th to Friday, June 28 th at Université Paris Descartes PROJECTED TIMETABLE Tuesday, June 18 th 9:30 am Registration in Room Gley on the 3rd floor 10:00 am Welcome and introductory information in Théâtre Lavoisier Patricia Gaspar ENP Director David Ogden ENP Spring School Coordinator 11:00 am Electronics of patch clamp recording Boris Barbour 2:00 pm Overview of methods in optical microscopy Brad Amos 3:30 pm Light sources Martin Thomas 5:00 pm Lasers for one and two photon excitation Wednesday, June 19 th Gail McConnell 9:30 am Detectors and CCD cameras Klaus Suhling 10:30 am Lecture demonstration - Resolution in the optical microscope Brad Amos 2:00 pm Microscopy at the Imaging platform at the Ecole Normale Supérieure ENS 1

2 Thursday, June 20 th 9:30 am One and two photon excitation, non-linear optical techniques Laurent Moreaux 11:00 am Scanning laser confocal and multi- beam microscopy Stefanie Reichelt 2:00 pm Confocal microscopy Friday, June 21 st at the ENS Imaging Platform. Lab visit **Experiments Start in Salle Richet at 1 :30 every day** 10:00 am Transgenic approaches to labeling neurons Jean Livet 11:30 am Holographic techniques in microscopy Valentina Emiliani 6:00 pm Small groups Demonstrations Viral injection Jonathan Bradley Lab Saturday, June 22 nd 10:00 am Fluorescent indicators of ion concentration Brandon Stell 11:30 am The HEKA patch clamps: software control of a patch clamp Monday June 24 th Hubert Affolter 10:00 am Extracellular recording Clement Lena 11:30 am Blind and Two photon-targeted patching (TPTP) in vivo Troy Margrie 2:00 pm Small groups Demonstrations In vivo patch clamp Lyle Graham lab 6:00 pm Methods for fast and flexible laser scanning microscopy Stéphane Dieudonné 2

3 Tuesday June 25 th 10:00 am Dynamic clamp of membrane conductance Lyle Graham 11:30 am Viral vectors in the study of nicotine action in vivo Uwe Maskos 2:00 pm Small groups Demonstration In vivo patch clamp Lyle Graham lab 6:00 pm Optical measurement of membrane potential Wednesday June 26 th Marco Canepari 10:00 am Optogenetic tools Thomas Knopfel 2:00 Small group Demonstrations Patch clamp and voltage clamp amplifiers and filters Reiner Polder 6:00 pm Image Analysis Christophe Pouzat Thursday June 27 th 10:00 am Imaging single molecules in live cells Marianne Renner 11:30 am Single channel kinetics underlying synaptic currents Marco Beato 6:00 pm Photolysis Friday June 28 th David Ogden DINNER PARTY at CROUS Mabillon 10:00 am Design of a two photon microscope Martin Oheim 11:30 am Endoscopic imaging Cathie Ventalon 3

4 Room Gley Morning coffee breaks at 9:30 am, lunch will be served from 1pm to 2pm, and the afternoon coffee breaks shall be at 3:30pm. Please make sure to keep the room tidy! CONTACT David OGDEN, Spring School Coordinator Université Paris Descartes Lab : VENUES Université Paris Descartes, 45 Rue des Saints Pères Paris 3 d floor Théâtre Lavoisier A, Salle Richet, Salle Gley. École Normale Supérieure, 46 rue d Ulm Paris Imaging Platform, Biology Building. Meeting point in the hall. CROUS Mabillon 3 rue Mabillon Paris 3 d floor 4

5 MAPS & TRANSPORTATION FROM CHARLES DE GAULLE AIRPORT TO THE HOTEL You will have to buy your return transport tickets to go from the airport/train station to the hotel and back. Please bring some cash (euro) for buying these transport tickets. American and most other non-european credit cards (without smart chips and without 4 digit pin numbers) will not work. RER-B underground train (Travel duration: approx. 1:10 hour) Ticket Single: Approx Billet Ile-de-France : Aéroport CDG Paris RER B (Blue) Line From Aéroport Charles de Gaulle 1 or 2 Direction Robinson or St-Rémy-lès-Chevreuse Up to Saint Michel Notre Dame Connection RER C (Yellow) Line From Saint Michel Notre Dame Direction Massy Palaiseau Versailles Chantiers or Dourdan - Saint Martin d Etampes Up to Ivry sur Seine Walk to Paris Ivry Hotel 4 rue Jean-Jacques Rousseau Ivry sur Seine Approx. 12 minutes see Map 1 FROM ORLY AIRPORT TO THE HOTEL By From Station Orly Sud (Orly) Direction Villejuif/ Louis Aragon Up to Pont de Rungis (Paray-Vieille-Poste) OR By Bus BIÈVRE BUS MOBILITÉS Navette Paris par le Train Direction Pont de Rungis - Aéroport d'orly From Station Orly Sud (Orly) Up to Pont de Rungis Aéroport d'orly RER (Thiais) 5

6 Connection walk 5 minutes RER C (Yellow) Line Direction Pontoise (NORA train) From Pont de Rungis Up to Ivry sur Seine FROM PARIS IVRY HOTEL TO UNIVERSITE DESCARTES A shuttle will pick you up one hour before the first class (8 :30 am or 09 :00am) at the Paris Ivry Hotel main entrance. Every morning from Tuesday 18 th to Tuesday 25 th, and to be confirmed during the second week. Using public transportation : RER C (Yellow) Line From Ivry sur Seine Direction Pontoise or Invalides or Versailles or Saint Quentin en Yvelines Up to Saint Michel Notre Dame Connection Metro Line 4 (Pink) From Saint Michel Notre Dame Direction Mairie de Montrouge To Saint-Germain des Prés See Maps 1 and 2 6

7 Map 1 Walking from Ivry sur Seine station to Ivry Hotel, 4 rue Jean-Jacques Rousseau Ivry sur Seine PARIS IVRY HOTEL 4 rue Jean-Jacques Rousseau Ivry sur Seine Tel : +33 (0) paris.ivry@sejours-affaires.com 7

8 Map 2 Walking from Saint-Germain-des-Prés metro station to Université Paris Descartes 45 rue des Saints Pères, 3 rd floor Théâtre Lavoisier A, Salle Richet, Salle Gley. 8

9 FROM UNIVERSITÉ PARIS DESCARTES TO ENS Going from Université Descartes to ENS : 25 minutes walk or by bus 84 : Walk 5 minutes from Descartes to rue de Sèvres ( Michel Debré bus stop), get off at Place du Panthéon, and then walk up rue d Ulm 5 minutes to ENS. Map 3 Walking from Université Descartes to Ecole Normale Supérieure 45 rue d Ulm. 25 minutes walk. Head east on Boulevard Saint-Germain to Passage de la Petite Boucherie Turn right to stay on Boulevard Saint-Germain; Continue on Carrefour de l'odéon; Turn left to stay on the Carrefour de l'odéon; Carrefour de l'odeon turns slightly right and becomes Rue Monsieur le Prince; Continue Place Edmond Rostand; At the roundabout, take the 3rd exit onto Boulevard Saint-Michel; Take a slight left onto Rue Gay-Lussac; Turn left on Rue Louis Thuillier; Turn right onto Rue d'ulm. Or by bus 84 : Walk 5 minutes from Descartes to rue de Sèvres ( Michel Debré bus stop), get off at Place du Panthéon, and then walk up rue d Ulm 5 minutes to ENS. 9

10 FROM UNIVERSITÉ PARIS DESCARTES TO DINNER PARTY AT THE CROUS MABILLON Going from Université Descartes to the CROUS Mabillon : 10 minutes walk. Map 4 Turn left on Boulevard Saint-Germain; Take the 4 th street on the right rue du Four, and and then turn on the second street on your left, rue de Mabillon. The restaurant is on the next corner, 3 rue Mabillon, and has wooden walls. Climb up to 3d floor! 10

11 A TIME OUT IN PARIS Exhibition Chagall entre guerre et paix at the Musée du Luxembourg Métro Saint-Germain-des-Prés Until July 28 th Fête de la Musique Free shows everywhere in Paris streets and concert halls Friday June 21 st Paris Jazz Festival at the Parc Floral Métro Château de Vincennes (Line 1) From June 8 th to July 28 th And more on 11

12 SATISFACTION SURVEY You are Speaker Student Program Quality Duration of sessions Overall Quality of presentations Balance between lectures and experiments Excellent Good Average Poor Organization Hotel Quality Catering Quality Communication Excellent Good Average Poor Remarks 12

13 AUTHORIZATION FOR QUOTING AND FOR FREE USE OF ONE S IMAGE(S) I The Undersigned... Address... please tick Authorize the shooting and the publication of the images on which I appear, indefinitely, and on different media (written, electronic, audiovisual). Accept that the ENP interviews me about the Spring School and quotes me in the article(s) to be published in its newsletter and website. I accept and understand that they are to be used for the promotion of the Optical Imaging and Electrophysiological Recording in Neuroscience Spring School organized by the Ecole des Neurosciences de Paris Ile de France and the DIM Cerveau & Pensée. I recognize that their potential use can not affect my privacy rights and are not likely to cause me any harm in any ways. DATE : Signature : Please complete and send back by mail to communication@paris-neuroscience.com or hand it over to Emma Cahill from the ENP before Thursday June 27 th. 13

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