Cryoinjury of Breast Cancer Cells in Tissue Equivalent Constructs

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Cryoinjury of Breast Cancer Cells in Tissue Equivalent Constructs"

Transcription

1 Cryoinjury of Breast Cancer Cells in Tissue Equivalent Constructs Bumsoo Han Department of Mechanical and Aerospace Engineering University of Texas at Arlington

2 Cryosurgery Minimally invasive surgical destruction of malignant or unwanted tissues by localized freezing Freezing by a fine surgical probe cooled with compressed argon (Joule-Thomson Effect) Intraoperative imaging provides surgeons with the progress of surgery (US, MRI and CT) Breast Cancer (Sanarus Medical) Prostate Cancer (Endocare & Oncura)

3 Challenges in Cryosurgery Incomplete killing zone between iceball edge and effective destruction zone Requirement of surgical margin to ensure complete tumor destruction Limited ability of monitoring effective destruction zone Establish thermal conditions of complete tissue destruction for various diseases. Make incomplete killing zone smaller by enhancing freezing injury within it.

4 Cryosurgical Injury Models Models Method Advantages Disadvantages Cell suspension Suspending cells in proper media Highest controllability No C-C nor C-ECM interaction Cell monolayer Monolayer or clones of cells on a substrate High controllability C-C interaction Cell-substrate interaction No C-ECM interaction No 3D effect Engineered artificial tissue (Present study) Seeding cells within a collagen gel matrix High controllability C-C interaction C-ECM interaction Partial 3D effect No vascular structure In vitro native tissue Harvesting tissue from a host animal C-C interaction C-ECM interaction 3D effect Medium controllability Non-functional vascular structure 2D in vivo tissue Growing tumor at the skin of a host animal (i.e. dorsal skin fold chamber) C-C interaction C-ECM interaction 3D effect Partial vascular structure Medium controllability No large blood vessels 3D in vivo tissue Growing tumor within a host animal C-C interaction C-ECM interaction 3D effect Full vascular structure Low controllability

5 Engineered Tumor Tissue MCF7 human breast cancer cells are seeded in type I collagen matrix and cultured. The cells grow within the collagen matrix. The cells are attached to collagen fibers which are working as extracellular matrix. Cell-cell, cell-ecm interaction, and cellbased healing response

6 Cryosurgery Simulator Thermocouple Cryosurgery Probe Upper Jig Jig Supporter Lower Jig Petri Dish Culture Media Engineered Tumor Tissue

7 Thermal History and Prediction during A Simulated Surgery Temperature ( C) Measurement Numerical model r=6mm r=6mm r=11mm r=11mm r=16mm r=16mm r=21mm r=21mm Time (sec) Numerical analysis based on one dimensional conduction equation solved by an enthalpy method Generally speaking, measured temperature profiles agree well with numerical prediction. Estimated edge of the ice ball 16mm

8 Freezing Injury of MCF7 in TEs Viability (%) Temperature ( C) Day 0 Day 1 Day Edge of Iceball Radial Location (mm) Radial Location (mm) Day 0 (3 hours) Less than 20% survival of MCF7 within 11mm No or minimal effects at the unfrozen locations Day 1 & 3 Tumor cells repopulate at the frozen locations. At 11mm, the tumor cell viability returns to the level of the unfrozen state. Tumor cell repopulation can be caused by cell reproduction and migration. Upper Fig - Post-thaw viability Lower Fig - End temperature

9 Histology - Complete Killing Zone Location: 3mm from the center of the probe Tapered probe site Mainly non-viable cells with hyperchromic nuclei Viability < 5% 10x Hypo-cellular density Rare voids in the matrix 40x

10 Histology - Incomplete Killing Zone Location: 11mm from the center of the probe Mixed viable and damaged cells Viability: 30 ~ 60% Moderate cellular density Numerous voids in the matrix It has a stranded appearance with increased staining suggesting concentrated collagen. 10x 40x

11 Histology - Live Zone Location: 18mm from the center of the probe Intact matrix structure Mainly viable cells Viability > 95% Highest cellular density 10x 40x

12 Tumor Necrosis Factor - α (TNF- α) Proinflammation cytokine Upregulate apoptotic signal pathways of tumor cells (caspases activation) At certain conditions, upregulate proliferation pathways of tumor cells (NF-kB activation) Toxic at high dosage Need to find a way to maximize the effectiveness with minimizing the toxicity

13 Effects of TNF-α treatment only Treatment with TNF-α Concentration : 100ng/ml Duration: 24 hours Cell number TNF-a treatment may stimulate the proliferation but the effect is not statistically significant. Viability No notable viability drop by the concentration and duration of current TNF-α treatment Cells/field Viability (%) TNF-! Treatment Control Day 0 Day 1 Day 3 Time Point TNF-! Treatment Control Day 0 Day 1 Day 3 Time Point

14 Freezing Injury with TNF-α Viability (%) Viability (%) Day 0 Day 1 Day Control Day 0 Day 1 Day 3 Radial Location (mm) Radial Location (mm) Day 0 Less than 10% survival of MCF7 within 11mm TNF-a slightly stimulates cell growth at the unfrozen locations. Day 1 & 3 Tumor cell repopulation is significantly inhibited at all frozen locations. At 11mm, the viability remains around 40% Upper Fig - Viability w TNF-α Lower Fig - Viability w/o TNF-α

15 Summary and Conclusion A cryoinjury model by use of tissue engineering technology was developed and its capability was demonstrated. Cryoinjury characteristics of human breast cancer cells were investigated. TNF-α was very effective to inhibit repopulation of MCF7 cells after cryosurgery. Future Research Application of current model to other cell lines Structural change due to freezing

16 Acknowledgements Matthew D. Egberg, Pung Haung David Swanlund James E. Coad, MD John C. Bischof, PhD Funding US DOD BCRP DAMD to BH BMEI Thermal Therapy Interest Group to JB

III. Cell destruction by freezing and cryoprobe development

III. Cell destruction by freezing and cryoprobe development III. Cell destruction by freezing and cryoprobe development 1 WHAT IS CRYOSURGERY? Cryoprobe Surface iceball formation Ice-ball Cryoprobe Cryosurgery of liver (Angular insertion) Cryosurgery of liver (Vertical

More information

Cryosurgery in Cancer Treatment: Questions and Answers. Key Points

Cryosurgery in Cancer Treatment: Questions and Answers. Key Points CANCER FACTS N a t i o n a l C a n c e r I n s t i t u t e N a t i o n a l I n s t i t u t e s o f H e a l t h D e p a r t m e n t o f H e a l t h a n d H u m a n S e r v i c e s Cryosurgery in Cancer

More information

Corporate Medical Policy

Corporate Medical Policy Corporate Medical Policy File Name: Origination: Last CAP Review: Next CAP Review: Last Review: microwave_tumor_ablation 12/2011 11/2015 11/2016 11/2015 Description of Procedure or Service Microwave ablation

More information

A PATIENT S GUIDE TO ABLATION THERAPY

A PATIENT S GUIDE TO ABLATION THERAPY A PATIENT S GUIDE TO ABLATION THERAPY THE DIVISION OF VASCULAR/INTERVENTIONAL RADIOLOGY THE ROBERT WOOD JOHNSON UNIVERSITY HOSPITAL Treatment options for patients with cancer continue to expand, providing

More information

Targeting Specific Cell Signaling Pathways for the Treatment of Malignant Peritoneal Mesothelioma

Targeting Specific Cell Signaling Pathways for the Treatment of Malignant Peritoneal Mesothelioma The Use of Kinase Inhibitors: Translational Lab Results Targeting Specific Cell Signaling Pathways for the Treatment of Malignant Peritoneal Mesothelioma Sheelu Varghese, Ph.D. H. Richard Alexander, M.D.

More information

Cancer: DNA Synthesis, Mitosis, and Meiosis

Cancer: DNA Synthesis, Mitosis, and Meiosis Chapter 5 Cancer: DNA Synthesis, Mitosis, and Meiosis Copyright 2007 Pearson Copyright Prentice Hall, 2007 Inc. Pearson Prentice Hall, Inc. 1 What Is Cancer? Benign tumors do not invade surrounding tissue

More information

Minimally Invasive Kidney Cryotherapy

Minimally Invasive Kidney Cryotherapy Minimally Invasive Kidney Cryotherapy [ mini mal ly in va sive ] minimally: [adv] to a minimal degree; the least or smallest possible invasive: [adj ] relating to a technique in which the body is entered

More information

Inflammatory Cytokine-induced Expression of Vasohibin-1 by Rheumatoid Synovial Fibroblasts

Inflammatory Cytokine-induced Expression of Vasohibin-1 by Rheumatoid Synovial Fibroblasts 9 6 6 958 Inflammatory Cytokine-induced Expression of Vasohibin- by Rheumatoid Synovial Fibroblasts a b c a a a a d e a* a a b c d e 5 6 6 9 5 56 67 7 ʼ ʼ ʼ ʼ ʼ ʼ ʼ ʼ 5 6 6 ʼ 5 75 989 6 69 8 95 56 56896

More information

Radiofrequency Ablation. A Patient s Guide

Radiofrequency Ablation. A Patient s Guide Radiofrequency Ablation A Patient s Guide TITLE Introduction to This Guide Each year, 530,000 people worldwide are diagnosed with liver disease.* Sometimes, the disease occurs first in the liver and is

More information

LCL-GRAFT TM - Innovative Large and Small Caliber Vascular Grafts for Coronary Bypass and Peripheral Vascular Surgery.

LCL-GRAFT TM - Innovative Large and Small Caliber Vascular Grafts for Coronary Bypass and Peripheral Vascular Surgery. LCL-GRAFT TM - Innovative Large and Small Caliber Vascular Grafts for Coronary Bypass and Peripheral Vascular Surgery. A. Perets 1, M. Li 1, P. Uttayarat 1, P. Pimton 1, A. Wu 2, R. J. Levy 3, R. J. Composto

More information

Coding for Prostate Cancer

Coding for Prostate Cancer Coding for Prostate Cancer Tracy L. Burney, M.D. Health First Physicians Urology September 19, 2012 Prostate Cancer Basics What is the Prostate and What Does it do? Walnut sized organ found only in men.

More information

JUMISC JUMISC. For further information visit our website:

JUMISC JUMISC. For further information visit our website: STEM CELL THERAPY COMPANY PROFILE & SCIENTIFIC ACTIVITIES For further information visit our website: WWW.CCMIJESUSUSON.COM 20 14 JUMISC JUMISC Carretera N-521, km. 41,8 10071 CÁCERES (SPAIN) Tel. (+34)

More information

1400 Telegraph Bloomfield Hills, MI 48302 248-334-6877-Phone number/248-334-6877-fax Number CANCER TREATMENT

1400 Telegraph Bloomfield Hills, MI 48302 248-334-6877-Phone number/248-334-6877-fax Number CANCER TREATMENT 1400 Telegraph Bloomfield Hills, MI 48302 248-334-6877-Phone number/248-334-6877-fax Number CANCER TREATMENT Learning that your pet has a diagnosis of cancer can be overwhelming. We realize that your pet

More information

Advanced MRI methods in diagnostics of spinal cord pathology

Advanced MRI methods in diagnostics of spinal cord pathology Advanced MRI methods in diagnostics of spinal cord pathology Stanisław Kwieciński Department of Magnetic Resonance MR IMAGING LAB MRI /MRS IN BIOMEDICAL RESEARCH ON HUMANS AND ANIMAL MODELS IN VIVO Equipment:

More information

Animal Cell Culture. Third Edition. A Practical Approach OXJORD VNIVVRSITY 1'RVSS

Animal Cell Culture. Third Edition. A Practical Approach OXJORD VNIVVRSITY 1'RVSS Animal Cell Culture Third Edition A Practical Approach Edited by John R. W. Masters 3rd Floor Research Laboratories, University College London OXJORD VNIVVRSITY 1'RVSS Contents List of protocols page xiii

More information

P4 Distribution of Cetuximab in Models of Human Lung Cancer

P4 Distribution of Cetuximab in Models of Human Lung Cancer Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology, University of Tübingen, Robert Bosch Foundation Stuttgart, Germany FORSYS Partner Project Predictive Cancer Therapy Project Meeting 14.10.09

More information

OBJECTIVES By the end of this segment, the community participant will be able to:

OBJECTIVES By the end of this segment, the community participant will be able to: Cancer 101: Cancer Diagnosis and Staging Linda U. Krebs, RN, PhD, AOCN, FAAN OCEAN Native Navigators and the Cancer Continuum (NNACC) (NCMHD R24MD002811) Cancer 101: Diagnosis & Staging (Watanabe-Galloway

More information

Growth of Animal Cells in Culture

Growth of Animal Cells in Culture Growth of Animal Cells in Culture The ability to study cells depends largely on how readily they can be grown and manipulated in the laboratory. Although the process is technically far more difficult than

More information

PowerLight LED Light Therapy. The FUTURE of corrective skin

PowerLight LED Light Therapy. The FUTURE of corrective skin PowerLight LED Light Therapy The FUTURE of corrective skin care TODAY LED facial treatments Effective when used with correct protocols Non thermal stimulation of collagen Increases circulation and lymphatic

More information

LifeNet Health Presented by James Clagett, PhD., Chief Science Officer LifeNet Health - The Best Keep Secret in Regenerative Medicine

LifeNet Health Presented by James Clagett, PhD., Chief Science Officer LifeNet Health - The Best Keep Secret in Regenerative Medicine Presented by James Clagett, PhD., Chief Science Officer - The Best Keep Secret in Regenerative Medicine Corporate Headquarters Virginia Beach, VA Who is? helps save lives and restore health for thousands

More information

BioArchive Freezing Curve for Cord Blood

BioArchive Freezing Curve for Cord Blood BioArchive Freezing Curve for Cord Blood Determination of the Specific Freezing in the BioArchive System to Provide the Highest Viability of Cord Blood MNC as Measured by -C Assays Introduction Freezing

More information

Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay. Introduction

Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay. Introduction Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay Application Note 2 Introduction Cancer is a leading cause of death worldwide, accounting for around 7.6 million

More information

What is Cancer? Cancer is a genetic disease: Cancer typically involves a change in gene expression/function:

What is Cancer? Cancer is a genetic disease: Cancer typically involves a change in gene expression/function: Cancer is a genetic disease: Inherited cancer Sporadic cancer What is Cancer? Cancer typically involves a change in gene expression/function: Qualitative change Quantitative change Any cancer causing genetic

More information

Osteoblast Differentiation and Mineralization

Osteoblast Differentiation and Mineralization Osteoblast Differentiation and Mineralization Application Note Background Osteoblasts are specialized fibroblasts that secrete and mineralize the bone matrix. They develop from mesenchymal precursors.

More information

Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay. Introduction

Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay. Introduction Routine Assessment of Cancer Cell Cytotoxicity in a Novel Three Dimensional Culture Assay Application Note Introduction Cancer is a leading cause of death worldwide, accounting for around 7.6 million deaths

More information

The 3D Petri Dish. A New Cutting-Edge Culture Format

The 3D Petri Dish. A New Cutting-Edge Culture Format The 3D Petri Dish A New Cutting-Edge Culture Format The 3D Petri Dish grows cells in three dimensions and forms multi-cellular microtissues that more closely mimic tissues in vivo The 3D Petri Dish is

More information

NCT Preliminary Report: Phase I Dose Escalating Study to Evaluate the Safety,

NCT Preliminary Report: Phase I Dose Escalating Study to Evaluate the Safety, NCT02020291 Preliminary Report: Phase I Dose Escalating Study to Evaluate the Safety, Tolerability, Pharmacokinetic and Pharmacodynamic Profiles of Foxy-5 in Patients with Metastatic Breast, Colon or Prostate

More information

Radius 24-Well Cell Migration Assay (Laminin Coated)

Radius 24-Well Cell Migration Assay (Laminin Coated) Product Manual Radius 24-Well Cell Migration Assay (Laminin Coated) Catalog Number CBA-125-LN 24 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Cell migration is a highly

More information

3D Cell Culture mimsys G

3D Cell Culture mimsys G 3D Cell Culture mimsys G xeno-free & nutrient permeable hydrogel for 3D cell culture mimsys G is a xeno-free and non-immunogenic, easy to handle hydrogel for cell encapsulation in 3D experiments in vitro

More information

LESSON 3.5 WORKBOOK. How do cancer cells evolve? Workbook Lesson 3.5

LESSON 3.5 WORKBOOK. How do cancer cells evolve? Workbook Lesson 3.5 LESSON 3.5 WORKBOOK How do cancer cells evolve? In this unit we have learned how normal cells can be transformed so that they stop behaving as part of a tissue community and become unresponsive to regulation.

More information

Contact Maryann Verrillo Diane Shnitzler (703) 691-1805. Interventional Radiology Treatments for Kidney Cancer

Contact Maryann Verrillo Diane Shnitzler (703) 691-1805. Interventional Radiology Treatments for Kidney Cancer Interventional Radiology Treatments for Kidney Cancer FACT SHEET Contact Maryann Verrillo Diane Shnitzler (703) 691-1805 Kidney cancer is the eighth most common cancer in men and the tenth in women. 1

More information

A Patient s Guide. A new and precise tool for destroying tumors in the brain.

A Patient s Guide. A new and precise tool for destroying tumors in the brain. A Patient s Guide A new and precise tool for destroying tumors in the brain www.mybraintumoroptions.com Your doctor has recommended you for a procedure with the NeuroBlate System because you have an abnormal

More information

TITLE: Treatment of Prostate Cancer with a DBP-MAF-Vitamin D Complex to Target Angiogenesis and Tumorigenesis

TITLE: Treatment of Prostate Cancer with a DBP-MAF-Vitamin D Complex to Target Angiogenesis and Tumorigenesis AD AWARD NUMBER: W81XWH-04-1-0010 TITLE: Treatment of Prostate Cancer with a DBP-MAF-Vitamin D Complex to Target Angiogenesis and Tumorigenesis PRINCIPAL INVESTIGATOR: Michael W. Fannon, Ph.D. CONTRACTING

More information

H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland School of Medicine Baltimore, Md

H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland School of Medicine Baltimore, Md Major Advances in Cancer Prevention, Diagnosis and Treatment~ Why Mesothelioma Leads the Way H. Richard Alexander, Jr., M.D. Department of Surgery and The Greenebaum Cancer Center University of Maryland

More information

Bottlenecks in Clinical Source Material Acquisition. Aby J. Mathew, PhD May 5, 2009 ISCT Annual Meeting San Diego, CA amathew@biolifesolutions.

Bottlenecks in Clinical Source Material Acquisition. Aby J. Mathew, PhD May 5, 2009 ISCT Annual Meeting San Diego, CA amathew@biolifesolutions. Bottlenecks in Clinical Source Material Acquisition Aby J. Mathew, PhD May 5, 2009 ISCT Annual Meeting San Diego, CA amathew@biolifesolutions.com Biopreservation What s the issue? Biopreservation considerations

More information

Biotechnology. Srivatsan Kidambi, Ph.D.

Biotechnology. Srivatsan Kidambi, Ph.D. Stem Stem Cell Cell Engineering-What, Biology and it Application Why, How?? to Biotechnology Srivatsan Kidambi, Ph.D. Assistant Professor Department of Chemical & Biomolecular Engineering University of

More information

Basic Science in Medicine

Basic Science in Medicine Medical Journal of th e Islamic Republic of Iran Volume 18 Number 3 Fall 1383 November 2004 Basic Science in Medicine ] EXPANSION OF HUMAN CORD BLOOD PRIMITIVE PROGENITORS IN SERUM-FREE MEDIA USING HUMAN

More information

Fighting the Battles: Conducting a Clinical Assay

Fighting the Battles: Conducting a Clinical Assay Fighting the Battles: Conducting a Clinical Assay 6 Vocabulary: In Vitro: studies in biology that are conducted using components of an organism that have been isolated from their usual biological surroundings

More information

FAK INHIBITOR DEFACTINIB (VS-6063) TARGETS MESOTHELIOMA CANCER STEM CELLS. Jonathan Pachter, Ph.D.

FAK INHIBITOR DEFACTINIB (VS-6063) TARGETS MESOTHELIOMA CANCER STEM CELLS. Jonathan Pachter, Ph.D. FAK INHIBITOR DEFACTINIB (VS-6063) TARGETS MESOTHELIOMA CANCER STEM CELLS Rationale for maintenance therapy after conventional therapy Jonathan Pachter, Ph.D. Vice President of Research, Verastem, Inc.

More information

Figure 1 Experimental workflow for performing a wound healing assay using the ibidi Culture-Insert.

Figure 1 Experimental workflow for performing a wound healing assay using the ibidi Culture-Insert. Wound Healing Assay with the ibidi Culture Insert in a µ-dish 35 mm 1. General information Cell migration plays a central role in many complex physiological and pathological processes. The wound healing

More information

Department of BioScience Technology Chung Yuan Christian University 2015/08/13

Department of BioScience Technology Chung Yuan Christian University 2015/08/13 Department of BioScience Technology Chung Yuan Christian University 2015/08/13 Cancer Cells Cancer, the 1st leading cause of death, is an example of a disease that arises from abnormalities in cell function

More information

Brigham and Women s Hospital, Boston, MA, USA; 2 Verastem, Inc., Boston, MA, USA

Brigham and Women s Hospital, Boston, MA, USA; 2 Verastem, Inc., Boston, MA, USA Determination of Biomarker Response in a Phase II Window of Opportunity Study of Defactinib (VS 6063), a Focal Adhesion Kinase (FAK) Inhibitor, in Patients with Resectable Malignant Pleural Mesothelioma

More information

Types of Cancers [-oma growth ]!

Types of Cancers [-oma growth ]! Cancer: disease of transcription factors and replication 1 Uncontrolled cell growth and division -> immortalized cells -> tumor growth -> metastasis (cells float away from tumor and spread throughout the

More information

Interventional Tumor Therapy Minimally Invasive, Maximally Effective

Interventional Tumor Therapy Minimally Invasive, Maximally Effective Cover Story Interventional Oncology Interventional Tumor Therapy Minimally Invasive, Maximally Effective There is a continuous expansion of indications for interventional therapies in oncology. Thanks

More information

Hyperthermia in Cancer Treatment: Questions and Answers. Key Points

Hyperthermia in Cancer Treatment: Questions and Answers. Key Points CANCER FACTS N a t i o n a l C a n c e r I n s t i t u t e N a t i o n a l I n s t i t u t e s o f H e a l t h D e p a r t m e n t o f H e a l t h a n d H u m a n S e r v i c e s Hyperthermia in Cancer

More information

Bone Graft Alternatives

Bone Graft Alternatives Bone Graft Alternatives North American Spine Society Public Education Series What Are Bone Grafts? Bone grafts are bone that is transplanted from one area of the skeleton to another to aid in healing,

More information

Novel Surgery for Inoperable Brain Tumors. A Patient Guide to NeuroBlate

Novel Surgery for Inoperable Brain Tumors. A Patient Guide to NeuroBlate Novel Surgery for Inoperable Brain Tumors A Patient Guide to NeuroBlate rose ella burkhardt brain tumor and neuro-oncology center Pioneering a Promising Surgical Treatment A brain tumor diagnosis is overwhelming

More information

MALIGNANT PLEURAL FLUIDS AID MESOTHELIOMA CELL GROWTH AND PROPAGATION

MALIGNANT PLEURAL FLUIDS AID MESOTHELIOMA CELL GROWTH AND PROPAGATION MALIGNANT PLEURAL FLUIDS AID MESOTHELIOMA CELL GROWTH AND PROPAGATION HUI MIN CHEAH PhD Candidate School of Medicine and Pharmacology University of Western Australia Lung Institute of Western Australia

More information

Cell Division Mitosis and the Cell Cycle

Cell Division Mitosis and the Cell Cycle Cell Division Mitosis and the Cell Cycle A Chromosome and Sister Chromatids Key Points About Chromosome Structure A chromosome consists of DNA that is wrapped around proteins (histones) and condensed Each

More information

Neoplasms. Review of Normal Cells. Bio 375 Pathophysiology

Neoplasms. Review of Normal Cells. Bio 375 Pathophysiology Neoplasms Bio 375 Pathophysiology Review of Normal Cells During its life span, each cell follows the basic cell cycle of growth and reproduction called mitosis The timing of each event varies with the

More information

PATIENT INFORMATION ABOUT ADJUVANT THERAPY AFTER THE WHIPPLE OPERATION FOR ADENOCARCINOMA ( CANCER ) OF THE PANCREAS AND RELATED SITES.

PATIENT INFORMATION ABOUT ADJUVANT THERAPY AFTER THE WHIPPLE OPERATION FOR ADENOCARCINOMA ( CANCER ) OF THE PANCREAS AND RELATED SITES. PATIENT INFORMATION ABOUT ADJUVANT THERAPY AFTER THE WHIPPLE OPERATION FOR ADENOCARCINOMA ( CANCER ) OF THE PANCREAS AND RELATED SITES. Radiation Oncology Sidney Kimmel Cancer Center at Johns Hopkins Last

More information

Update on Clinical Trials and Foundation Funded Grants

Update on Clinical Trials and Foundation Funded Grants Update on Clinical Trials and Foundation Funded Grants Mary Hesdorffer, MS, APRN-BC Medical Liaison Meso Foundation www.curemeso.org Delivering the Diagnosis Delivering the Diagnosis Day 1 Taking control

More information

Cryopreservation of Umbilical Cord Tissue for Stem Cell Harvesting Executive Summary Figure 1. Cross section of normal umbilical cord.

Cryopreservation of Umbilical Cord Tissue for Stem Cell Harvesting Executive Summary Figure 1. Cross section of normal umbilical cord. Cryopreservation of Umbilical Cord Tissue for Stem Cell Harvesting By Steven Buchwald, Nicole Chiu, Melvin Chu, Hesed Kim, Calvin Yeang BEE 453 5/2/03 Executive Summary Stem cell transplantation has become

More information

San Diego Stem Cell Treatment Center Frequently Asked Questions

San Diego Stem Cell Treatment Center Frequently Asked Questions San Diego Stem Cell Treatment Center Frequently Asked Questions What is a Stem Cell? A stem cell is basically any cell that can replicate and differentiate. This means the cell can not only multiply, but

More information

Demystifying Stem Cells. Brent Bost M.D., CPA, MBA, FACOG

Demystifying Stem Cells. Brent Bost M.D., CPA, MBA, FACOG Demystifying Stem Cells Brent Bost M.D., CPA, MBA, FACOG Disclaimers Author currently serves on Board of Directors of CordTrack Most applications discussed today are not currently FDA approved and are,

More information

Brain Cancer. This reference summary will help you understand how brain tumors are diagnosed and what options are available to treat them.

Brain Cancer. This reference summary will help you understand how brain tumors are diagnosed and what options are available to treat them. Brain Cancer Introduction Brain tumors are not rare. Thousands of people are diagnosed every year with tumors of the brain and the rest of the nervous system. The diagnosis and treatment of brain tumors

More information

A Genetic Analysis of Rheumatoid Arthritis

A Genetic Analysis of Rheumatoid Arthritis A Genetic Analysis of Rheumatoid Arthritis Introduction to Rheumatoid Arthritis: Classification and Diagnosis Rheumatoid arthritis is a chronic inflammatory disorder that affects mainly synovial joints.

More information

Chapter 3. Treatment Options

Chapter 3. Treatment Options Chapter 3. Treatment Options With contributions from Nancy L. Brown, Ph.D., Palo Alto Medical Foundation Research Institute; Patrick Swift, M.D., Alta Bates Comprehensive Cancer Program; D. Jeffrey Demanes,

More information

Understanding. Pancreatic Cancer

Understanding. Pancreatic Cancer Understanding Pancreatic Cancer Understanding Pancreatic Cancer The Pancreas The pancreas is an organ that is about 6 inches long. It s located deep in your belly between your stomach and backbone. Your

More information

CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format)

CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format) Product Manual CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format) Catalog Number CBA-050 48 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction

More information

East Lancashire Surgical Robot

East Lancashire Surgical Robot East Lancashire Surgical Robot For ALL patients in Lancashire Prostate cancer claims the life of one man every hour and by 2030 will be the most common cancer; it is already the most common male cancer.

More information

Moving Beyond RECIST

Moving Beyond RECIST Moving Beyond RECIST Ihab R. Kamel, M.D., Ph.D. ikamel@jhmi.edu Associate Professor Clinical Director, MRI Department of Radiology The Johns Hopkins University School of Medicine Outline Standard measures

More information

Metastatic Cancer: Questions and Answers. Key Points

Metastatic Cancer: Questions and Answers. Key Points CANCER FACTS N a t i o n a l C a n c e r I n s t i t u t e N a t i o n a l I n s t i t u t e s o f H e a l t h D e p a r t m e n t o f H e a l t h a n d H u m a n S e r v i c e s Metastatic Cancer: Questions

More information

What is Glioblastoma? How is GBM classified according to the WHO Grading System? What risk factors pertain to GBM?

What is Glioblastoma? How is GBM classified according to the WHO Grading System? What risk factors pertain to GBM? GBM (English) What is Glioblastoma? Glioblastoma or glioblastoma multiforme is one of the most common brain tumors accounting for approximately 12 to 15 percent of all brain tumors. The name of the tumor

More information

Radiation Therapy for Prostate Cancer

Radiation Therapy for Prostate Cancer Radiation Therapy for Prostate Cancer Introduction Cancer of the prostate is the most common form of cancer that affects men. About 240,000 American men are diagnosed with prostate cancer every year. Your

More information

HER2 Testing in Breast Cancer

HER2 Testing in Breast Cancer HER2 Testing in Breast Cancer GAIL H. VANCE, M.D. AGT MEETING JUNE 13, 2014 LOUISVILLE, KENTUCKY No Conflict of Interest to Report Human Epidermal Growth Factor Receptor 2-HER2 Human epidermal growth factor

More information

Breast Cancer. CSC Cancer Experience Registry Member, breast cancer

Breast Cancer. CSC Cancer Experience Registry Member, breast cancer ESSENTIALS Breast Cancer Take things one step at a time. Try not to be overwhelmed by the tidal wave of technical information coming your way. Finally you know your body best; you have to be your own advocate.

More information

Immunotherapy of Uveal Melanoma

Immunotherapy of Uveal Melanoma Eye Am Not Alone (EANA) Patient Retreat Saturday Session - March 3, 2012 Immunotherapy of Uveal Melanoma Do not distribute or copy without the express permission of the Ocular Melanoma Foundation (OMF).

More information

CLINICAL POLICY Department: Medical Management Document Name: Opdivo Reference Number: CP.PHAR.121 Effective Date: 07/15

CLINICAL POLICY Department: Medical Management Document Name: Opdivo Reference Number: CP.PHAR.121 Effective Date: 07/15 Page: 1 of 6 IMPORTANT REMINDER This Clinical Policy has been developed by appropriately experienced and licensed health care professionals based on a thorough review and consideration of generally accepted

More information

Gallbladder Diseases and Problems

Gallbladder Diseases and Problems Gallbladder Diseases and Problems Introduction Your gallbladder is a pear-shaped organ under your liver. It stores bile, a fluid made by your liver to digest fat. There are many diseases and problems that

More information

Kim Mammen Abhinav Jaiswal Chris3an Medical College & Hospital Ludhiana India

Kim Mammen Abhinav Jaiswal Chris3an Medical College & Hospital Ludhiana India Kim Mammen Abhinav Jaiswal Chris3an Medical College & Hospital Ludhiana India } In the case of prostate cancer, a new, more conserva3ve form of monitoring called Ac3ve Surveillance is emerging which might

More information

Current Status and Perspectives of Radiation Therapy for Breast Cancer

Current Status and Perspectives of Radiation Therapy for Breast Cancer Breast Cancer Current Status and Perspectives of Radiation Therapy for Breast Cancer JMAJ 45(10): 434 439, 2002 Masahiro HIRAOKA, Masaki KOKUBO, Chikako YAMAMOTO and Michihide MITSUMORI Department of Therapeutic

More information

Effets biologiques des facteurs de croissance sur la régénération musculaire

Effets biologiques des facteurs de croissance sur la régénération musculaire Effets biologiques des facteurs de croissance sur la régénération musculaire Xavier Bigard (MD, PhD) Professeur Agrégé du Val-de-Grâce Institut de Recherche Biomédicale des Armées (IRBA) La Tronche (Grenoble)

More information

MesoPDT. Photodynamic Therapy for malignant pleural mesothelioma ONCO-THAI. Image Assisted Laser Therapy for Oncology

MesoPDT. Photodynamic Therapy for malignant pleural mesothelioma ONCO-THAI. Image Assisted Laser Therapy for Oncology MesoPDT Photodynamic Therapy for malignant pleural mesothelioma ONCO-THAI Image Assisted Laser Therapy for Oncology Unité Inserm ONCO-THAI «Image Assisted Laser Therapy for Oncology» Inserm ONCO-THAI "Image

More information

co-sponsored by the Health & Physical Education Department, the Health Services Office, and the Student Development Center

co-sponsored by the Health & Physical Education Department, the Health Services Office, and the Student Development Center Cancer is a group of more than 100 related diseases. Normally, cells grow and divide to produce more cells to keep the body healthy. Sometimes, this process goes wrong. New cells form when the body doesn

More information

Ductal Carcinoma in Situ (DCIS)

Ductal Carcinoma in Situ (DCIS) Diagnosis and Treatment of Patients with Primary and Metastatic Breast Cancer Ductal Carcinoma in Situ (DCIS) Ductal Carcinoma in Situ DCIS Version 2002: Gerber Version 2003 2009: Audretsch / Brunnert

More information

Introduction Breast cancer is cancer that starts in the cells of the breast. Breast cancer happens mainly in women. But men can get it too.

Introduction Breast cancer is cancer that starts in the cells of the breast. Breast cancer happens mainly in women. But men can get it too. Male Breast Cancer Introduction Breast cancer is cancer that starts in the cells of the breast. Breast cancer happens mainly in women. But men can get it too. Many people do not know that men can get breast

More information

Breast Implants: Local Complications and Adverse Outcomes

Breast Implants: Local Complications and Adverse Outcomes Breast Implants: Local Complications and Adverse Outcomes This booklet highlights the most common problems associated with silicone gel-filled and saline-filled breast implants: those that occur in the

More information

Empower 3D cell culture with simplicity and versatility. VitroGel TM 3D. a ready-to-use hydrogel system for 3D cell culture and beyond

Empower 3D cell culture with simplicity and versatility. VitroGel TM 3D. a ready-to-use hydrogel system for 3D cell culture and beyond Empower 3D cell culture with simplicity and versatility VitroGel TM 3D a ready-to-use hydrogel system for 3D cell culture and beyond VitroGel TM 3D is an animal origin-free polysaccharide hydrogel system

More information

A918: Prostate: adenocarcinoma

A918: Prostate: adenocarcinoma A918: Prostate: adenocarcinoma General facts of prostate cancer The prostate is about the size of a walnut. It is just below the bladder and in front of the rectum. The tube that carries urine (the urethra)

More information

Purification and Expansion of Hematopoietic Stem Cells Based on Proteins Expressed by a Novel Stromal Cell Population

Purification and Expansion of Hematopoietic Stem Cells Based on Proteins Expressed by a Novel Stromal Cell Population Purification and Expansion of Hematopoietic Stem Cells Based on Proteins Expressed by a Novel Stromal Cell Population Our bodies are constantly killing old, nonfunctional, and unneeded cells and making

More information

Accelerated Partial Breast Irradiation (APBI) for Breast Cancer [Pre-authorization Required]

Accelerated Partial Breast Irradiation (APBI) for Breast Cancer [Pre-authorization Required] Accelerated Partial Breast Irradiation (APBI) for Breast Cancer [Pre-authorization Required] Medical Policy: MP-SU-01-11 Original Effective Date: February 24, 2011 Reviewed: February 24, 2012 Revised:

More information

The lungs What is lung cancer? How common is it? Risks & symptoms Diagnosis & treatment options

The lungs What is lung cancer? How common is it? Risks & symptoms Diagnosis & treatment options Why We re Here The lungs What is lung cancer? How common is it? Risks & symptoms Diagnosis & treatment options What Are Lungs? What Do They Do? 1 Located in the chest Allow you to breathe Provide oxygen

More information

A912: Kidney, Renal cell carcinoma

A912: Kidney, Renal cell carcinoma A912: Kidney, Renal cell carcinoma General facts of kidney cancer Renal cell carcinoma, a form of kidney cancer that involves cancerous changes in the cells of the renal tubule, is the most common type

More information

A Patient s Guide to the Calypso System for Breast Cancer Treatment

A Patient s Guide to the Calypso System for Breast Cancer Treatment A Patient s Guide to the Calypso System for Breast Cancer Treatment Contents Introduction How Radiation Works To Treat Cancer Radiation Treatment for Breast Cancer Special Concerns for Treating the Left

More information

Unit 1 Higher Human Biology Summary Notes

Unit 1 Higher Human Biology Summary Notes Unit 1 Higher Human Biology Summary Notes a. Cells tissues organs body systems Division of labour occurs in multicellular organisms (rather than each cell carrying out every function) Most cells become

More information

There are four areas where you can expect changes to occur as your hormone therapy progresses.

There are four areas where you can expect changes to occur as your hormone therapy progresses. You are considering taking testosterone, so you should learn about some of the risks, expectations, long term considerations, and medications associated with medical transition. If is very important to

More information

Simplifying Oncology Care

Simplifying Oncology Care Simplifying Oncology Care March 2009 Research Philips Research focuses on ultrasound-mediated drug delivery Research Philips Research focuses on ultrasound-mediated drug delivery Philips Research and its

More information

dedicated to curing BREAST CANCER

dedicated to curing BREAST CANCER dedicated to curing BREAST CANCER When you are diagnosed with breast cancer, you need a team of specialists who will share their knowledge of breast disease and the latest treatments available. At Cancer

More information

to Know About Your Partner s

to Know About Your Partner s What You Need to Know About Your Partner s Prostate Cancer. A Guide for Wives, Partners, and the Men They Love. Providing Support for Your Partner Prostate cancer can be a physical and emotional challenge

More information

Chapter 22: The Lymphatic System and Immunity

Chapter 22: The Lymphatic System and Immunity Chapter 22: The Lymphatic System and Immunity Introduction Immune system the body s defenses against pathogens that produce disease 2 types of immunity Nonspecific immune mechanisms (Innate immunity) Provide

More information

Mesothelioma. 1995-2013, The Patient Education Institute, Inc. www.x-plain.com ocft0101 Last reviewed: 03/21/2013 1

Mesothelioma. 1995-2013, The Patient Education Institute, Inc. www.x-plain.com ocft0101 Last reviewed: 03/21/2013 1 Mesothelioma Introduction Mesothelioma is a type of cancer. It starts in the tissue that lines your lungs, stomach, heart, and other organs. This tissue is called mesothelium. Most people who get this

More information

Innovations in Kidney Cancer

Innovations in Kidney Cancer Innovations in Kidney Cancer Guest Expert: Harriet, MD Associate Professor of Medical Oncology Edward, MD Assistant Professor of Surgical Urology www.wnpr.org www.yalecancercenter.org Welcome to Yale Cancer

More information

Stem Cell Research: Adult or Somatic Stem Cells

Stem Cell Research: Adult or Somatic Stem Cells Chiang 1 Stem Cell Research: Adult or Somatic Stem Cells Abstract Kelly Chiang Cluster 7 Dr. LeFebvre 07/26/10 Over the past few decades, stem cells have been a controversial topic in the scientific field.

More information

Cold as an adjunctive therapy for headache

Cold as an adjunctive therapy for headache Cold as an adjunctive therapy for headache Seymour Diamond, MD Frederick G. Freltag, DO Preview Patients with acute headache are often so vexed by pain that they seek out numerous physicians and headache

More information

Cancer SBL101. James Gomes School of Biological Sciences Indian Institute of Technology Delhi

Cancer SBL101. James Gomes School of Biological Sciences Indian Institute of Technology Delhi Cancer SBL101 James Gomes School of Biological Sciences Indian Institute of Technology Delhi All Figures in this Lecture are taken from 1. Molecular biology of the cell / Bruce Alberts et al., 5th ed.

More information

CONTRACTING ORGANIZATION: University of Alabama at Birmingham Birmingham, AL 35294

CONTRACTING ORGANIZATION: University of Alabama at Birmingham Birmingham, AL 35294 AD Award Number: W81XWH-08-1-0030 TITLE: Regulation of Prostate Cancer Bone Metastasis by DKK1 PRINCIPAL INVESTIGATOR: Gregory A. Clines, M.D., Ph.D. CONTRACTING ORGANIZATION: University of Alabama at

More information

Monoclonal Antibodies in Cancer. Ralph Schwall, PhD Associate Director, Translational Oncology Genentech, Inc.

Monoclonal Antibodies in Cancer. Ralph Schwall, PhD Associate Director, Translational Oncology Genentech, Inc. Monoclonal Antibodies in Cancer Ralph Schwall, PhD Associate Director, Translational Oncology Genentech, Inc. Disclaimer I had nothing to do with Herceptin Using lessons learned in new antibody projects

More information

Policy Wording. Together, all the way.

Policy Wording. Together, all the way. Cancer cover Policy Wording Together, all the way. Cancer Cover Insurance Policy Wording 1. Introducing your Policy 2. What is Cancer Cover Insurance? 3. About your Policy 4. Some terms defined 5. What

More information

Information Model Requirements of Post-Coordinated SNOMED CT Expressions for Structured Pathology Reports

Information Model Requirements of Post-Coordinated SNOMED CT Expressions for Structured Pathology Reports Information Model Requirements of Post-Coordinated SNOMED CT Expressions for Structured Pathology Reports W. Scott Campbell, Ph.D., MBA James R. Campbell, MD Acknowledgements Steven H. Hinrichs, MD Chairman

More information