1 BTI Implant systems Scientific Dossier Prince Felipe Award for Technological Innovation
3 Introduction Introduction This scientific dossier summarises the series of indexed international articles published over the last 15 years on the range of products and technologies developed by BTI Biotechnology Institute. It highlights the vast amount of scientific evidence that backs the biosafety and effectiveness of plasma rich in growth factors (PRGF -Endoret ) in many fields of medicine, with a particular focus on oral and maxillofacial surgery and oral implantology. This autologous technology has revolutionised the field of personalised regenerative medicine, as with the patient's blood we can obtain different therapeutic formulations rich in growth factors, the application of which encourages healing and tissue regeneration, reducing pain and inflammation. Many publications show the predictability and safety of BTI dental implants and of the surgical techniques (sinus elevation, split, biological drilling, etc) developed under a biological philosophy by Dr. Eduardo Anitua. This dossier also reviews the therapeutic potential of PRGF -Endoret in other fields of medicine in which our research team has been a pioneer at a worldwide level: orthopaedic surgery, treatment of chronic ulcers and facial regeneration, among others.
4 What are growth factors and how do they act? Growth factors are a set of substances that carry out an important function in intercellular communication. They carry out a large number of biological functions among which cellular proliferation is important, though they also decisively affect cellular survival, migration, differentiation and even apoptosis. Growth factors carry out their function at very low concentrations in body fluids and tissues, in the region of pico or nanograms. They act by binding to receptors located on the cell membrane that transmit the signal from the exterior to the interior of the cell, through the coupling of different protein kinases that are phosphorylated and which regulate a signalling cascade that ends up with the activation of one or more genes.
5 Growth factors are a set of substances that are fundamental for communication between cells. BTI Biotechnology Institute S.L.
6 How can tissues be regenerated? The process of tissue regeneration includes a complex set of biological events controlled by the action and synergy of a cocktail of growth factors. There are three agents involved in tissue regeneration: the cellular component, a combination of multiple biological mediators that include growth factors and cytokines among others and a matrix or scaffold that gives the new tissue under construction support. After an injury or tissue damage, they are activated and coordinate a large number of intercellular or intracellular paths with the aim of restoring the integrity of the tissue and its hemostasis. Growth factors are also necessary to promote angiogenesis or the formation of blood vessels that will supply oxygen and nutrients to the damaged tissue. Another fundamental aspect to be considered in the regeneration of a tissue is the development of a scaffold that acts as a provisional extracellular matrix and therefore houses the cells as well as locally presenting the biochemical, physical and structural signals that allow the anchorage of the cellular motility machinery. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol. Rev. 2003;83:
7 BTI Biotechnology Institute S.L. The tissue regeneration process is based on the combination of cells, growth factors and a matrix or Scaffold. Orive BIOGRAPHY: G, Anitua * Anitua E, et al. E, Biomaterials Sánchez M, Nurden for promoting AT, Nurden brain P, protection, Orive G, Andía repair I. New and insights regeneration. into and Nat Rev novel Neurosci. applications 2009;9: for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
8 PRGF -Endoret : A new concept in personalised regenerative medicine Over the last 15 years, the detailed study of platelets, of biological mediators contained in them and of the formulations aimed at allowing the administration and therapeutic use of growth factors and autologous biomaterials has allowed significant progress and has greatly increased the versatility and therapeutic possibilities of Plasma Rich in Growth Factors (PRGF -Endoret ) technology. PRGF -Endoret technology is based on the preparation of platelet-rich 100% autologous plasma the application of which to damaged tissue areas speeds up the regeneration of a large number of tissues without any adverse effects. Plasma rich in growth factors (PRGF - Endoret ) is a personalised technology that has revolutionised the field of regenerative medicine. This article, published in one of the most prestigious journals in the field of biotechnology, summarises the most important clinical results obtained with PRGF -Endoret. Its application over the last decade has extended to many fields of medicine, from oral and maxillofacial surgery to dermatology, cosmetics, orthopaedic surgery and sports medicine, and more recently to ophthalmology. Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol. 2006;24:
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10 How is PRGF -Endoret obtained? The set of therapeutic formulations of PRGF -Endoret are obtained by means of a simple protocol based on a tiny volume of the patient's blood. The blood is centrifuged (580g) in 9 ml citrated tubes during 8 minutes allowing the separation of red and white blood cells from the platelet-rich plasma. The two fractions of PRGF -Endoret are separated from the rest of the blood components by means of the plasma transfer device (PTD). Later, and prior to its therapeutic application, the fractions of PRGF -Endoret are activated with calcium chloride, leading to a series of therapeutic formulations. The process to obtain PRGF -Endoret is simple and easily reproducible. DEMONSTRATION VIDEO Scan this code with your mobile phone to watch the video. Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants. 1999;14:
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12 Versatility of PRGF -Endoret This article, published in of the most important scientific journals in the field of biomaterials, focuses on the enormous versatility that PRGF -Endoret technology offers, as by using the patient's blood we can obtain up to 4 biocompatible formulations: a. PRGF -Endoret supernatant: used to cultivate primary cells and stem cells in the laboratory, it is also the base of a new collyrium for treating a large number of pathologies of the occular surface. b. Liquid PRGF -Endoret : Ideal for infiltrations in skin, musculoskeletal system tissues, TMJ, etc. It is the perfect tool to bioactivate dental implants and prostheses of all types with the aim of accelerating their osseointegration. c. PRGF -Endoret clot or scaffold : Ideal to fill in defects and promote tissue regeneration: post-extraction, treatment of ulcers, tissue engineering, etc. d. Fibrin membrane: due to its hemostatic properties it is the best biomaterial to seal defects and stimulate epithelisation. Anitua E, Sánchez M, Orive G, Andía I. The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials. 2007;28:
13 a b BTI Biotechnology Institute S.L. c d The versatility of PRGF -Endoret technology allows you to obtain up to 4 autologous formulations. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
14 Why does PRGF -Endoret work? The biological activity of the different formulations obtained with PRGF -Endoret technology is based on two fundamental pillars. On the one hand, the content in plasma and in particular platelet growth factors whose action regulates the main processes involved in tissue regeneration. the most important in the field of pharmacology, talks about the therapeutic potential of PRGF -Endoret technology and in particular describes how the perfect synergy between growth factors and fibrin is a key aspect when explaining the clinical results obtained with this autologous technology. On the other, the fibrin matrix, which is used as a provisional structure to house the cells and control the release kinetics of the growth factors present in PRGF -Endoret. Anitua E, Sánchez M, Orive G, Andia I. Delivering growth factors for therapeutics. Trends Pharmacol Sci. 2008;29:37-41 This article published in the prestigious journal Trends in Pharmacological Science, BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
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16 What makes PRGF -Endoret different to other platelet-rich plasmas? PRGF -Endoret is the first 100% autologous platelet-rich plasma to be described in literature worldwide. It is, likewise, a pioneering technology in translational regenerative medicine. Over 15 years of research, added to its exclusive properties, make PRGF -Endoret a unique technique. PRGF -Endoret is prepared with small volumes of the patient's blood and does not require the use of thrombin or chemical agents for its activation. Unlike other products, it does not include white blood cells (leukocytes) in its composition, which gives it more effective anti-inflammatory properties. It is the most versatile technology, as its multiple formulations offer a large number of therapeutic applications. In short, as shown by the series of letters to the editor published during recent years, we can define PRGF -Endoret as a platelet-rich autologous plasma whose effectiveness and safety have been widely proven. However, it is important to remember that not all platelet-rich plasmas are PRGF -Endoret. DEMONSTRATION VIDEO Scan this code with your mobile phone to watch the video.
17 Anitua E, Sánchez M, Orive G, Andía I. Shedding light in the controversial terminology for platelet rich products. J Biomed Mater Res A. 2009;90: Sánchez M, Anitua E, Andia I. Poor standardization in platelet-rich therapies hampers advancement. Arthroscopy. 2010;26: Anitua E, Sánchez M, Orive G. The importance of understanding what is platelet-rich growth factor (PRGF) and what is not. J Shoulder Elbow Surg. 2011;20: Anitua E, Sánchez M, Prado R, Orive G. Plasma rich in growth factors: the pioneering autologous technology for tissue regeneration. J Biomed Mater Res A. 2011;97:536. BTI Biotechnology Institute S.L. Anitua E, Sánchez M, Prado R, Orive G. The P makes the difference in plasma rich in growth factors (PRGF) technology. Platelets. 2011;22: BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
18 The pillars of PRGF -Endoret : growth factors PRGF -Endoret contains a cocktail of autologous growth factors that proceed from both the plasma and the platelets. In fact, the platelets have a complex storage system in the form of intracellular granules that allow them to transport a large number of biologically active molecules. According to some authors, this list of proteins and peptides can come close to 500 molecules. Alpha (α) granules are the most abundant as there are around 40 to 80 alpha granules per platelet, but they are also the ones with the greatest retention capacity. In addition, they contain a series of antibacterial proteins that are generically called thrombocidines and which are lethal for a large variety of bacterial species. However, it is important to remember that the plasma contains important growth factors and that the combination of the plasma and platelet factors is a key element in the biological action of PRGF -Endoret. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost. 2004;91:4-15.
19 BTI Biotechnology Institute S.L. El PRGF -Endoret contains hundreds of molecules including plasma and platelet growth factors. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
20 Platelets and PRGF -Endoret In a couple of revision articles, our research team along with the Nurden Doctors from the Reference Centre for Platelet Disorders in France, have characterised the protein content of platelets in order to learn about the set of molecules present in PRGF -Endoret formulations. factor, EGF: epidermal growth factor or angiopoietin-1 among others. They release in parallel antibacterial molecules and specific growth factors that act on the mobilisation of progenitor cells from the bone marrow or from peripheral niches. Platelets release substances that promote tissue regeneration and which modulate both angiogenesis and inflammation. Important among other factors we have PDGF: plateletderived growth factor, TGF-ß: transforming growth factor ß, bfgf: basic fibroblast growth factor, VEGF: vascular endothelial growth Nurden AT, Nurden P, Sanchez M, Andia I, Anitua E. Platelets and wound healing. Front Biosci. 2008;13:
21 BTI Biotechnology Institute S.L. Platelets release growth factors responsible for coordinating the regenerative tissue response. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
22 The pillars of PRGF -Endoret : fibrin as a biomaterial Calcium acts as a cofactor in the activation process of PRGF -Endoret, which allows the conversion of the fibrinogen of the plasma into fibrin, generating a gel or clot with important biological functions. On the one hand, fibrin is an excellent matrix to maintain and house the cells, it acts as a provisional scaffold while the definitive tissue is regenerated and acts as a continuous growth factor release system. It is therefore a biocompatible and autologous sponge full of growth factors and cytokines that will permit a progressive release of them during several weeks. The fibrin obtained with PRGF -Endoret technology is probably the best biomaterial for encouraging tissue regeneration. DEMONSTRATION VIDEO Scan this code with your mobile phone to watch the video. Anitua E, Sanchez M, Nurden AT, Zalduendo M, de la Fuente M, Orive G, Azofra J, Andia I. Autologous fibrin matrices: a potential source of biological mediators that modulate tendon cell activities. J Biomed Mater Res A. 2006;77:
23 BTI Biotechnology Institute S.L. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
24 Biological and regenerative potential of PRGF -Endoret In over a decade of preclinical research, during which tens of cellular phenotypes were studied, we have managed to discover and understand the multiple biological functions that the set of therapeutic formulations of PRGF -Endoret carry out. The biological mediators of PRGF -Endoret stimulate and encourage such important processes for tissue regeneration as cellular proliferation and migration, chemotaxis (or the call from a distance for cells to go to the location of the injury), inflammation and the auto/paracrine synthesis of new molecules with biological activity. DEMONSTRATION VIDEO Scan this code with your mobile phone to watch the video. Anitua E, Sanchez M, Merayo-Lloves J, De la Fuente M, Muruzabal F, Orive G. Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts TGF-beta1- Induced myodifferentiation. Invest Ophthalmol Vis Sci. 2011;52: Anitua E, Prado R, Orive G. Bilateral sinus elevation evaluating plasma rich in growth factors technology: a report of five cases. Clin Implant Dent Relat Res. 2012;14:51-60 Bendinelli P, Matteucci E, Dogliotti G, Corsi MM, Banfi G, Maroni P, Desiderio MA. Molecular basis of anti-inflammatory action of platelet-rich plasma on human chondrocytes: mechanisms of NF-κB inhibition via HGF. J Cell Physiol. 2012;225:
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26 PRGF -Endoret in the field of stem cells The growing interest in the range of biological options that PRGF -Endoret offers has even reached the field of stem cells. Stem or progenitor cells are characterised on the one hand by their unlimited capacity for proliferation, and on the other by the possibility of undergoing asymmetrical division (that is, self-renovation) maintaining their stemness while at the same time they can differentiate to diverse types of cells. There are different types of stem cells depending on their origin and their anatomical location. There is evidence that the content of biologically active agents in PRGF -Endoret affects the mobilisation, adhesion, proliferation, survival, activation and differentiation of mesenchymal stem cells and other subtypes of precursor cells. In addition, the cocktail of growth factors of PRGF -Endoret is an ideal resource for the cultivation and expansion of stem cells in the laboratory.
27 BTI Biotechnology Institute S.L. de Boer HC, Verseyden C, Ulfman LH, Zwaginga JJ, Bot I, Biessen EA, Rabelink TJ, van Zonneveld AJ. Fibrin and activated platelets cooperatively guide stem cells to a vascular injury and promote differentiation towards an endothelial cell phenotype. Arterioscler Thromb Vasc Biol. 2006;26: Kajikawa Y, Morihara T, Sakamoto H, Matsuda KI, Oshima Y, Yoshida A, Nagae M, Arai Y, Kawata M, Kubo T. Plateletrich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. J Cell Physiol. 2008;215: Zhang J, Wang JH. Platelet-rich plasma releasate promotes differentiation of tendon stem cells into active tenocytes. Am J Sports Med. 2010;38: Cenni E, Avnet S, Fotia C, Salerno M, Baldini N. Platelet-rich plasma impairs osteoclast generation from human precursors of peripheral blood. J Orthop Res. 2010;28: Chen L, Dong SW, Liu JP, Tao X, Tang KL, Xu JZ. Synergy of tendon stem cells and platelet-rich plasma in tendon healing. J Orthop Res BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
28 Mechanism of action of PRGF -Endoret The use of growth factors and autologous fibrin for regenerative purposes represents a new approach to personalised medicine that a large number of patients could benefit from. In this paper, published in one of the most important journals of drug delivery, they discuss the mechanisms of action through which PRGF -Endoret produces its multiple therapeutic effects. Likewise, also important is the angiogenic action of the growth factors which is crucial to start regeneration. Last of all, though no less important, its anti-inflammatory and antibacterial properties are a key element. The stimulation of cell proliferation and migration along with the call to circulating cells to come to the location of the injury are basic aspects of the action of PRGF -Endoret. Anitua E, Orive G. Endogenous regenerative technology using plasma- and platelet-derived growth factors. J Control Release
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30 Bacteriostatic potential of PRGF -Endoret Our research team has proven that PRGF -Endoret presents bacteriostatic activity with a large number of bacterial and fungal strains. This is because the platelets contain a series of antibacterial proteins called thrombocidines. These proteins are part of a wider family known as defensins, and they are of a cationic nature, which will allow them to bind to and alter bacterial membranes. In addition to thrombocidines, platelets transport and release other antimicrobial peptides among which we should mention platelet factor 4, RANTES, tissue activating peptide 3, the basic protein of platelets, thymosin β-4, and fibrinopeptides A and B. In a recent paper we could see that the bacteriostatic potential of platelet-rich growth factors is due both to the antimicrobial peptides and to the fibrin, and not to the presence of leukocytes in their composition. In fact, the bacteriostatic effect of PRGF -Endoret is identical to that of a platelet and leukocyte-rich plasma. Another important conclusion of this study was to confirm how the inclusion of leukocytes notably alters the structure and uniformity of the fibrin matrix. Anitua E, Alonso R, Girbau C, Aguirre JJ, Muruzabal F, Orive G. Antibacterial effect of plasma rich in growth factors (PRGF) against Staphylococcus aureus and epidermidis strains. Clin Exp Dermatol
31 BTI Biotechnology Institute S.L. PRGF -Endoret presents bacteriostatic activity with a variety of bacterial strains. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
32 Therapeutic applications of PRGF -Endoret This revision article is a reference in international bibliography as it is the journal with the greatest scientific impact. It is about the therapeutic potential of plateletrich plasma, and in this specific case, PRGF - Endoret. This dossier summarises the preclinical and clinical scientific articles that endorse the biosafety and efficacy of PRGF -Endoret in many fields of medicine. The use of growth factors and autologous fibrin for regenerative purposes represents a new approach to personalised medicine that a large number of patients could benefit from. Anitua E, Sánchez M, Orive G. Potential of endogenous regenerative technology for in situ regenerative medicine. Adv Drug Deliv Rev. 2010;62:
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34 Field of Oral Surgery Introduction The versatility of PRGF -Endoret has allowed its use in the treatment of a wide range of clinical problems within the field of oral and maxillofacial surgery. An example of this is the following series of applications developed by our research team over the last two decades and summarised in the scientific dossier. From the treatment of the alveolus post-extraction to the use of this technology in gingival recessions or in the humectation of dental implants to encourage their osseointegration, the range of therapeutic possibilities is enormous. The possibility that PRGF -Endoret offers of working with a liquid formulation, with a three-dimensional matrix or a with retracted fibrin membrane drastically increases the number of therapeutic options and applications. We must not forget that thanks to its agglutinant properties, PRGF -Endoret technology is the perfect fellow traveller to prepare grafts, whether they are autologous bone, allogenic or any other biomaterial for bone regeneration.
35 BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
36 Surface bioactivation Preclinical Research In the late 90s Dr. Anitua developed a pioneering technique to humectate implants and bioactivate their surface with fibrin and autologous growth factors. In a simple procedure, the implants are soaked in freshly activated liquid PRGF- Endoret and in this way a fibrin membrane full of growth factors is generated that will encourage a faster osseointegration of the implants. The nano-rough surface of BTI implants is specially designed to boost the biological effects of PRGF -Endoret. DEMONSTRATION VIDEO Scan this code with your mobile phone to watch the video. Anitua EA. Enhancement of osseointegration by generating a dynamic implant surface. J Oral Implantol. 2006;32:72-76.
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38 Surface bioactivation Preclinical Research In this study carried out with research animals the aim was to determine whether the humectation of the implant surfaces with PRGF -Endoret encouraged their osseointegration. To this end a total of 23 implants were inserted in the tibia/radius of 3 goats; 13 of them were previously humectated with PRGF -Endoret while the other 10 were not (control). 8 weeks later, a histomorphometric analysis was carried out on the bone biopsies of the sacrificed animals and we observed that the value of the bone-implant contact (BIC) was 51.28% in the animals humectated with PRGF -Endoret, as opposed to 21.89% in the implants that were not humectated, generating a significantly greater area in the former (p<0.01). These results prove how the humectation of implants with PRGF -Endoret encourages their faster osseointegration. Anitua E, Orive G, Pla R, Roman P, Serrano V, Andía I. The effects of PRGF on bone regeneration and on titanium implant osseointegration in goats: a histologic and histomorphometric study. J Biomed Mater Res A. 2009;91:
39 BTI Biotechnology Institute S.L. Humectation of BTI implants with PRGF -Endoret encourages faster osseointegration. BIOGRAPHY: * Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andía I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006 May;24(5):
40 Surface bioactivation Preclinical Research This new study carried out on research animals evaluated the effect of humectation of dental implants with PRGF -Endoret to encourage and accelerate their osseointegration. To this end 2 dogs were used in which 12 implants were inserted, 6 on each side of the mandible, humectating the implants of one side with PRGF -Endoret, and placing the implants of the opposite side without humectation (control). After 12 weeks, the implants were extracted along with the adjacent bone for a histologicalhistomorphometric analysis. The results showed that the implants humectated with PRGF -Endoret presented higher levels of BIC (bone-impant contact). We also observed higher values of trabecular bone thickness and bone maturity in the areas treated with PRGF -Endoret. Birang R, Tavakoli M, Shahabouei M, Torabi A, Dargahi A, Soolari A. Investigation of peri-implant bone healing using autologous plasma rich in growth factors in the canine mandible after 12 weeks: a pilot study. Open Dent J. 2011;5:
41 BTI Biotechnology Institute S.L. The aim of this study was to assess both the morphology and the composition of the interface formed by the implants activated with PRGF -Endoret. Both features are of capital importance for later regenerative events as both the morphology and the composition of the interface allow the modulation, among other aspects, of the balance between inflammation and regeneration around the implant. In this article we could see through electron, atomic and confocal microscopy that the bioactivation of implants with PRGF - Endoret generates a three-dimensional scaffold with a multitude of platelets, proteins and growth factors. In addition, the composition of the interface of the surfaces bioactivated with PRGF -Endoret is specific, containing platelet and protein elements different to those of other surfaces assessed. The specificity of both the morphology and the composition of the interfaces formed with implants bioactivated with PRGF - Endoret is very possibly the reason behind its beneficial clinical results. Tejero R, Rossbach P, Keller B, Anitua E, Reviakine I. Implant surfaces activated with plasma rich in growth factors: time of flight secondary ion mass spectrometry and principal component analysis study
42 The biological potential of PRGF -Endoret Preclinical Research In this laboratory test we analysed whether the new fraction F2 of PRGF -Endoret produces the same biological effect as the old fraction F3. To this end the potential was evaluated of the different formulations of PRGF -Endoret in the stimulation of different biological processes in human gingival fibroblasts, including the proliferation, migration and adhesión of fibroblasts, as well as the autocrine release of some angiogenic factors and components of the cellular matrix. The results observed showed that PRGF - Endoret significantly increased the cellular proliferation, migration and adhesion of gingival fibroblasts. These results showed how PRGF -Endoret is capable of promoting the regeneration of gingival connective tissue and highlighted the biological potential of the new fraction F2 of PRGF -Endoret. Anitua E, Troya M, Orive G. Plasma Rich in Growth Factors Promotes Gingival Tissue Regeneration by Stimulating Fibroblast Proliferation and Migration and by Blocking TGF-β1-Induced Myodifferentiation. J Periodontol
43 BTI Biotechnology Institute S.L. In another study carried out on human osteoblasts, again we saw the biological potential of the new fraction F2 of PRGF - Endoret and it was compared to the previous one, F3. To do so we evaluated: proliferation, migration, chemotaxis, autocrine secretion of growth factors and the production of components of the extracellular matrix. The results showed that the new F2 managed to increase the proliferation, migration and chemotaxis of the osteoblasts. In addition, the autocrine expression of two relevant pro-angiogenic factors: VEGF and HGF was significantly improved, and that of three osteoblastic activity markers: procollagen I (PC), osteocalcin (OC) and alkaline phosphatase (ALP). These results showed how the new fraction F2 of PRGF -Endoret stimulates some of the biological processes of the main cells responsible for bone regeneration and its effects are biologically comparable to the previous one, F3. Anitua E, Tejero R, Zalduendo MM, Orive G. Plasma Rich in Growth Factors Promotes Bone Tissue Regeneration by Stimulating Proliferation, Migration and Differentiation of Primary Human Osteoblasts
Endogenous Regenerative Technology oral surgery www.bti-implant.us Regenerative Medicine LEADER IN REGENERATIVE MEDICINE BTI Biotechnology Institute is a Spanish biomedicine company focused on the development
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IMPLANTOLOGY Dental implant is an artificial tooth root replacement and is used in prosthodontics dentistry. There are several types. The most widely accepted and successful is the osseointegrated implant.
Journal of Data Science 6(2008), 591-599 Life Table Analysis for Evaluating Curative-effect of One-stage Non-submerged Dental Implant in Taiwan Miin-Jye Wen 1, Chuen-Chyi Tseng 2 and Cheng K. Lee 3 1 National
Original Article Comparison of Peri-Implant Bone Loss and Survival of Maxillary Intrasinus and Extrasinus Implants After 2 Years AR. Rokn 1,2, AAR. Rasouli Ghahroudi 3,4, S. Hemati 5, A. Soolari 6 1 Associate
1/2 point CDT documented scientific credit. See Page 41. Implants in your Laboratory: Abutment Design By Leon Hermanides, CDT A patient s anatomical limitations have the greatest predictive value for successful
Dental Implant Options in Atrophic Jaws Orthopedic Application Jay B. Reznick, D.M.D., M.D. Diplomate, American Board of Oral and Maxillofacial Surgery Tarzana, CA Endopore Dental Implant System Screw-Type
Replacement of the upper left central incisor with a Straumann Bone Level Implant and a Straumann Customized Ceramic Abutment by Dr. Ronald Jung and Master Dental Technician Xavier Zahno Initial situation
J Clin Periodontol 2008; 35 (Suppl. 8): 168 172 doi: 10.1111/j.1600-051X.2008.01268.x Advances in bone augmentation to enable dental implant placement: Consensus Report of the Sixth European Workshop on
Modern Tooth Replacement Strategies & Digital Workflow Case Studies by Dr Maurice Salama, DMD AS PUBLISHED BY Dentistry Today, June 2014 Complete Implant Restoration System FACTS: Implant Dentistry Has
More than a fixed rehabilitation. A reason to smile. In combination with: Patient expectations drive dental treatments for fixed edentulous immediate restorations. Patients today have increasingly high
CDT 2015 Code Change Summary New codes effective 1/1/2015 Code Nomenclature Delta Dental Policy D0171 Re-Evaluation Post Operative Office Visit Not a Covered Benefit D0351 3D Photographic Image Not a Covered
Chapter 6 Aesthetical improvement Use of one-piece type implants 1. Improving esthetics with one-piece implant Director of Kinebuchi Dental Clinic Takao Kinebuchi Aesthetics of two-piece two-stage type
The SATURN implant by Cortex Dental Industries By Dr. Zvi Laster DMD W e P r o v e I t E v e r y D a y A case report using a newly designed implant specifically designed for immediate post-extraction loading
Stem Stem Cell Cell Engineering-What, Biology and it Application Why, How?? to Biotechnology Srivatsan Kidambi, Ph.D. Assistant Professor Department of Chemical & Biomolecular Engineering University of
BioHorizons Education Programme 2015 SPMP14328GB Rev A November 2014 Contents The Role of Implants in Restorative Dentistry An Introduction to Contemporary Implant Prosthodontics Sinus Elevation Socket
VARIA The immediate post-extractional insertion of screw implants in dental practice Ioan Sîrbu, Emanuel Bratu, Mihai Sandulescu, Gabriela Tanase, Ion Popovici Bucuresti, Romania Summary This article presents
Residency Competency and Proficiency Statements 1. REQUEST AND RESPOND TO REQUESTS FOR CONSULTATIONS Identify needs and make referrals to appropriate health care providers for the treatment of physiologic,
I overview Bisphosphonate therapy and osteonecrosis of the jaw Authors_Johannes D. Bähr, Prof. Dr Dr Peter Stoll & Dr Georg Bach, Germany _Introduction Fig. 1_Structural formula of pyrophosphate and basic
Dent. J. 2015, 3, 15-23; doi:10.3390/dj3010015 Article OPEN ACCESS dentistry journal ISSN 2304-6767 www.mdpi.com/journal/dentistry Six-Year Survival and Early Failure Rate of 2918 Implants with Hydrophobic
Effets biologiques des facteurs de croissance sur la régénération musculaire Xavier Bigard (MD, PhD) Professeur Agrégé du Val-de-Grâce Institut de Recherche Biomédicale des Armées (IRBA) La Tronche (Grenoble)
1257 Lancaster Ave Berwyn, PA 19312 Tel: 610-722-5542 CHIUN-LIN (STEVEN), LIU D.D.S., D.M.D. School of Dental Medicine CURRICULUM VITAE Summer, 2012 Education: 1987-1993 D.D.S. Kaohsiung Medical University
Saudi Fellowship In Dental Implant (SF-DI) Prepared and Updated by Dr. Arwa AL-Sayed Consultant Periodontics and Dental Implants M E M B E R S Dr. Arwa AL-Sayed Dr. Abdulhadi Abanmy Dr. Ali AL-Ghamdi Dr.
All-on-4 treatment concept with NobelSpeedy Groovy Product overview Immediate Function for high patient satisfaction Immediately loaded fixed provisional prosthesis on the day of surgery. Immediate improvement
All new applicants must meet the following requirements as approved by the UNMH Board of Trustees effective: 09/26/2014 INSTRUCTIONS Applicant: Check off the "Requested" box for each privilege requested.
Bartolomeo Operti, MD and Dr Tiziano Tealdo Accelerated recovery of post-operative dental implant patients by means of pulsed shortwave (SWT) therapy: Bartolomeo Operti, MD Chief of Anesthesia Department
Inflammation and Healing BIO 375 Pathophysiology Review of Normal Defenses Review of Normal Capillary Exchange 1 Inflammation Inflammation is a biochemical and cellular process that occurs in vascularized
BISPHOSPHONATE RELATED OSTEONECROSIS OF THE JAW (BRONJ) BISPHOSPHONATES AND WHAT HAPPENS TO BONE VINCENT E. DIFABIO, DDS, MS MEMBER OF THE COMMITTEE ON HEALTHCARE AND ADVOCACY FROM THE AMERICAN ASSOCIATION
PowerLight LED Light Therapy The FUTURE of corrective skin care TODAY LED facial treatments Effective when used with correct protocols Non thermal stimulation of collagen Increases circulation and lymphatic
The Very Best Reason for Dental Implants Gentle jawbone reconstruction with SonicWeld Rx There s a time where nothing can really hurt us It is the time where everything grows and thrives and even our teeth
Dental Implants - the tooth replacement solution Are missing teeth causing you to miss out on life? Missing teeth and loose dentures make too many people sit on the sidelines and let life pass them by.
April Masterclasses in UAE, Kuwait & Saudi Arabia NEW INNOVATIVE METHOD OF LIPO-PLASMA LIFT FACIAL A procedure using both platelet growth factors, adipose micronized tissue and stem cell to induce an effective
Improving Esthetics with Sequential Treatment Planning and Implant-Retained Dentures by Timothy F. Kosinski, DDS, MAGD While oral function is the primary concern for most patients, the importance of esthetics
What is a dental implant? Today, the preferred method of tooth replacement is a dental implant. They replace missing tooth roots and form a stable foundation for replacement teeth that look, feel and function
Module I: The Basic Principles of Stem Cells 1. Basics of Stem Cells a. Understanding the development of embryonic stem cells i. Embryonic stem cells ii. Embryonic germ cells iii. Differentiated stem cell
Essentials of Anatomy & Physiology, 4th Edition Martini / Bartholomew The Cardiovascular System: Blood PowerPoint Lecture Outlines prepared by Alan Magid, Duke University Slides 1 to 54 The Functions of
Advitech Advitech is a life sciences & technology company Its mission is to discover and commercialize proprietary and evidence-based natural health products Focus on milk, whey and bovine colostrum R&D,
Zimmer Trabecular Metal Dental Implant TRABECULAR METAL MATERIAL: Designed to Enhance Secondary Stability Through Bone Ingrowth. Artistic Rendering osseoincorporation TRABECULAR METAL MATERIAL Designed
IMPLANTS IN FOCUS PLANNING FOR IMPLANT RESTORATIONS Replacing a missing maxillary central incisor with a dental implant can be the most demanding restoration in dentistry, so it s important to consider
PREPARATION OF MOUTH FOR REMOVABLE PARTIAL DENTURES Dr. Mazen kanout Mouth preparation includes procedures in four categories: 1. Oral Surgical Preparation. 2. Conditioning of Abused and Irritated Tissue.
1 Presenter: Dr. Sana Ehsen Supervisors: Dr. Farhan Raza Khan 2 A dental implant (also known as an endosseous implant or fixture) is a surgical component that interfaces with the bone of the jaw to support
Taking the Mystique out of Implant Dentistry Dr. Michael Weinberg B.Sc., DDS, FICOI What is Restorative Implant Dentistry? Restorative implant dentistry involves taking a few simple mechanical principles
Foundation Revolutionary Bone Augmentation Material Thinking ahead. Focused on life. Regional Partner Stimulates New Bone Growth Foundation is a collagen-based, bone filling augmentation material for use
PATIENT INFORM CONSENT for IMPLANT RESTORATION Rev 04.2012 Implant placement and restoration involves two major stages: surgical placement of the implant(s) followed by the restoration of the implant after
PRP: Platelet Rich Plasma Wes Sutter, DVM, ACVS October 2007 I. Abstract/introduction Slow or poor quality healing of bones, tendon & ligaments and other soft tissues are common clinical problems in horses.
Rehabilitation of a complex case with zirconium dental Authors_Dr Andrea Enrico Borgonovo, Dr Marcello Dolci, Dr Rachele Censi, Dr Oscar Arnaboldi, Dr Virna Vavassori & Prof Carlo Maiorana, Italy _Introduction
Osseoincorporation: Adding Dimension to Implant Dentistry Zimmer Trabecular Metal Dental Implant Osseoincorporation Adding Dimension The Zimmer Trabecular Metal Dental Implant combines the popular features
THE RESORBABLE MATRIX Relax. Remaix. The membrane of choice for reliable bone and tissue regeneration Remaix properties Remaix is a resorbable barrier membrane for use in guided bone regeneration (GBR)
INFUSE Bone Graft Oral Maxillofacial Bone Grafting Procedures Dental Products Advisory Panel Committee Ed Chin, DPh Group Director, Regulatory Affairs Medtronic Spinal and Biologics 1 INFUSE Bone Graft
Don t Let Life Pass You By Because Of Missing Teeth Ask For Dental Implant Solutions From BIOMET 3i Scan With Your Smartphone! In order to scan QR codes, your mobile device must have a QR code reader installed.
CASE REPORT Implant Bone Rings. One-Stage Three-Dimensional Bone Transplant Technique: A Case Report Mark R. Stevens, DDS 1 * Hany A. Emam, MS 2 Mahmoud E. L. Alaily, MS 3 Mohamed Sharawy, PhD 4 A variety
Course Number: 108.2 Flapless Implant Surgery for Replacement of Posterior Teeth Authored by J. Steven Cloyd, DDS Upon successful completion of this CE activity 1 CE credit hour may be awarded A Peer-Reviewed
Unless otherwise noted, the content of this course material is licensed under a Creative Commons Attribution - Non-Commercial - Share Alike 3.0 License. Copyright 2008, Dr. Jeff Shotwell. The following
Teeth and Dental Implants: When to save, and when to extract. One of the most difficult decisions a restorative dentist has to make is when to refer a patient for extraction and placement of dental implants.